DE2532779C3 - Verfahren zum elektroquantitativen Bestimmen von Proteinen - Google Patents
Verfahren zum elektroquantitativen Bestimmen von ProteinenInfo
- Publication number
- DE2532779C3 DE2532779C3 DE2532779A DE2532779A DE2532779C3 DE 2532779 C3 DE2532779 C3 DE 2532779C3 DE 2532779 A DE2532779 A DE 2532779A DE 2532779 A DE2532779 A DE 2532779A DE 2532779 C3 DE2532779 C3 DE 2532779C3
- Authority
- DE
- Germany
- Prior art keywords
- protein
- glutaraldehyde
- reaction product
- solution
- albumin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 102000004169 proteins and genes Human genes 0.000 title claims description 53
- 108090000623 proteins and genes Proteins 0.000 title claims description 53
- 238000000034 method Methods 0.000 title claims description 47
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims description 30
- 102000009027 Albumins Human genes 0.000 claims description 27
- 108010088751 Albumins Proteins 0.000 claims description 27
- 210000002966 serum Anatomy 0.000 claims description 27
- 238000001962 electrophoresis Methods 0.000 claims description 23
- 239000000243 solution Substances 0.000 claims description 23
- 239000007795 chemical reaction product Substances 0.000 claims description 20
- 108060003951 Immunoglobulin Proteins 0.000 claims description 19
- 239000000499 gel Substances 0.000 claims description 19
- 102000018358 immunoglobulin Human genes 0.000 claims description 19
- 239000003795 chemical substances by application Substances 0.000 claims description 10
- 102000007562 Serum Albumin Human genes 0.000 claims description 9
- 108010071390 Serum Albumin Proteins 0.000 claims description 9
- 239000000872 buffer Substances 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 229920000936 Agarose Polymers 0.000 claims description 7
- 230000001588 bifunctional effect Effects 0.000 claims description 7
- 239000012153 distilled water Substances 0.000 claims description 6
- 239000011541 reaction mixture Substances 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 5
- 239000002253 acid Substances 0.000 claims description 4
- 239000011543 agarose gel Substances 0.000 claims description 4
- 239000011159 matrix material Substances 0.000 claims description 4
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 4
- 239000012062 aqueous buffer Substances 0.000 claims description 3
- 230000015572 biosynthetic process Effects 0.000 claims description 3
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 3
- 239000004327 boric acid Substances 0.000 claims description 3
- 229910052708 sodium Inorganic materials 0.000 claims description 3
- 239000011734 sodium Substances 0.000 claims description 3
- 150000001502 aryl halides Chemical class 0.000 claims description 2
- 125000000217 alkyl group Chemical group 0.000 claims 2
- 150000001875 compounds Chemical class 0.000 claims 1
- 239000006258 conductive agent Substances 0.000 claims 1
- 150000002463 imidates Chemical class 0.000 claims 1
- 235000018102 proteins Nutrition 0.000 description 44
- 239000000523 sample Substances 0.000 description 17
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 14
- 238000006243 chemical reaction Methods 0.000 description 11
- 102000006395 Globulins Human genes 0.000 description 10
- 108010044091 Globulins Proteins 0.000 description 10
- 229940072221 immunoglobulins Drugs 0.000 description 9
- 239000000427 antigen Substances 0.000 description 8
- 102000036639 antigens Human genes 0.000 description 8
- 108091007433 antigens Proteins 0.000 description 8
- 239000007853 buffer solution Substances 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 230000029142 excretion Effects 0.000 description 7
- FTOAOBMCPZCFFF-UHFFFAOYSA-N 5,5-diethylbarbituric acid Chemical compound CCC1(CC)C(=O)NC(=O)NC1=O FTOAOBMCPZCFFF-UHFFFAOYSA-N 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 230000008030 elimination Effects 0.000 description 5
- 238000003379 elimination reaction Methods 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 102000004506 Blood Proteins Human genes 0.000 description 4
- 108010017384 Blood Proteins Proteins 0.000 description 4
- 230000000890 antigenic effect Effects 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 108010074605 gamma-Globulins Proteins 0.000 description 4
- 241000283073 Equus caballus Species 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 230000000951 immunodiffusion Effects 0.000 description 3
- 238000000760 immunoelectrophoresis Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 210000002381 plasma Anatomy 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 230000005856 abnormality Effects 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 230000006735 deficit Effects 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000012085 test solution Substances 0.000 description 2
- RTBFRGCFXZNCOE-UHFFFAOYSA-N 1-methylsulfonylpiperidin-4-one Chemical compound CS(=O)(=O)N1CCC(=O)CC1 RTBFRGCFXZNCOE-UHFFFAOYSA-N 0.000 description 1
- FALRKNHUBBKYCC-UHFFFAOYSA-N 2-(chloromethyl)pyridine-3-carbonitrile Chemical compound ClCC1=NC=CC=C1C#N FALRKNHUBBKYCC-UHFFFAOYSA-N 0.000 description 1
- 241001432959 Chernes Species 0.000 description 1
- ZNZYKNKBJPZETN-WELNAUFTSA-N Dialdehyde 11678 Chemical compound N1C2=CC=CC=C2C2=C1[C@H](C[C@H](/C(=C/O)C(=O)OC)[C@@H](C=C)C=O)NCC2 ZNZYKNKBJPZETN-WELNAUFTSA-N 0.000 description 1
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical class [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 235000015842 Hesperis Nutrition 0.000 description 1
- 235000012633 Iberis amara Nutrition 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 240000006240 Linum usitatissimum Species 0.000 description 1
- 201000005505 Measles Diseases 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- JFCQEDHGNNZCLN-UHFFFAOYSA-N anhydrous glutaric acid Natural products OC(=O)CCCC(O)=O JFCQEDHGNNZCLN-UHFFFAOYSA-N 0.000 description 1
- HNYOPLTXPVRDBG-UHFFFAOYSA-N barbituric acid Chemical compound O=C1CC(=O)NC(=O)N1 HNYOPLTXPVRDBG-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000002981 blocking agent Substances 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000003431 cross linking reagent Substances 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- ZLFRJHOBQVVTOJ-UHFFFAOYSA-N dimethyl hexanediimidate Chemical compound COC(=N)CCCCC(=N)OC ZLFRJHOBQVVTOJ-UHFFFAOYSA-N 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000005868 electrolysis reaction Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 231100000283 hepatitis Toxicity 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- FPGCYQVKNKEGRQ-UHFFFAOYSA-N methyl 6,17,18-trimethoxy-1,3,11,12,14,15,16,17,18,19,20,21-dodecahydroyohimban-19-carboxylate Chemical compound COC1=CC=C2C(CCN3CC4CC(C(C(C4CC33)C(=O)OC)OC)OC)=C3NC2=C1 FPGCYQVKNKEGRQ-UHFFFAOYSA-N 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 238000001814 protein method Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 229940014800 succinic anhydride Drugs 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/807—Apparatus included in process claim, e.g. physical support structures
- Y10S436/809—Multifield plates or multicontainer arrays
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/826—Additives, e.g. buffers, diluents, preservatives
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US491069A US3912610A (en) | 1974-07-23 | 1974-07-23 | Method for electroquantitative determination of proteins |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| DE2532779A1 DE2532779A1 (de) | 1976-02-12 |
| DE2532779B2 DE2532779B2 (de) | 1980-09-25 |
| DE2532779C3 true DE2532779C3 (de) | 1981-10-15 |
Family
ID=23950657
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE2532779A Expired DE2532779C3 (de) | 1974-07-23 | 1975-07-22 | Verfahren zum elektroquantitativen Bestimmen von Proteinen |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US3912610A (ref) |
| JP (1) | JPS5529385B2 (ref) |
| CA (1) | CA1049402A (ref) |
| DE (1) | DE2532779C3 (ref) |
| FR (1) | FR2280076A1 (ref) |
| GB (1) | GB1492899A (ref) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4018662A (en) * | 1975-01-03 | 1977-04-19 | Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. | Method and apparatus for simultaneous quantitative analysis of several constituents in a sample |
| US3984533A (en) * | 1975-11-13 | 1976-10-05 | General Electric Company | Electrophoretic method of detecting antigen-antibody reaction |
| US4105521A (en) * | 1977-09-21 | 1978-08-08 | Helena Laboratories Corporation | Clinical procedure for measuring lipoprotein cholesterols |
| US4292154A (en) * | 1979-11-07 | 1981-09-29 | Gelman Sciences, Inc. | Buffer composition and method for the electrophoretic separation of proteins |
| US4321119A (en) * | 1979-11-07 | 1982-03-23 | Gelman Sciences, Inc. | Buffer composition and method for the electrophoretic separation of proteins |
| US4321120A (en) * | 1980-03-19 | 1982-03-23 | Nardi Ronald V | Process for detecting proteins specific to hypertension in mammals |
| US4322274A (en) * | 1980-08-28 | 1982-03-30 | Wilson Gregory B | Method of diagnosing cystic fibrosis patients and asymptomatic carrier of the cystic fibrosis gene |
| US4501816A (en) * | 1982-09-30 | 1985-02-26 | Aberra Molla | Method of determining immunoglobulin levels in mammals |
| US4559120A (en) * | 1983-03-23 | 1985-12-17 | Rush-Presbyterian-St. Luke's Medical Center | Agarose gel electrophoresis technique for the determination of amylase isoenzymes |
| US4668363A (en) * | 1984-03-16 | 1987-05-26 | Beckman Instruments, Inc. | Immunofixation electrophoresis process |
| DE454763T1 (de) * | 1989-01-13 | 1992-03-19 | Fmc Corp., Philadelphia, Pa. | Polysaccharid-resolutionsgele und gelsysteme fuer elektrophorese durch aufstapeln. |
| JP2798418B2 (ja) * | 1989-05-16 | 1998-09-17 | 帝國製薬株式会社 | 免疫電気泳動用ゲル作製器 |
| US5753094A (en) * | 1995-09-20 | 1998-05-19 | Beckman Instruments, Inc. | Borate storage buffer and sample diluent |
| US6660149B1 (en) * | 1997-10-24 | 2003-12-09 | Beckman Coulter, Inc. | Multichannel microscale system for high throughput preparative separation with comprehensive collection and analysis |
| WO2017149122A1 (en) * | 2016-03-04 | 2017-09-08 | Morphosys Ag | Clinical assessment of m-protein response in multiple myeloma |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE316928B (ref) * | 1966-11-25 | 1969-11-03 | Kabi Ab | |
| US3607695A (en) * | 1969-02-14 | 1971-09-21 | Pfizer & Co C | Hemoglobinopathy controls |
| SE357891B (ref) * | 1970-04-08 | 1973-07-16 | Lkb Produkter Ab | |
| DE2137617C3 (de) * | 1971-07-28 | 1979-01-04 | Grubhofer, Nikolaus, Dr., 6900 Heidelberg | Verfahren zur Herstellung wäßriger Ampholytlösungen oder deren Gemischen |
| BE789188A (fr) * | 1971-09-24 | 1973-01-15 | Orion Yhtymae Oy | Procede pour la determination quantitative et qualitative de molecules ayant des proprietes antigenes |
-
1974
- 1974-07-23 US US491069A patent/US3912610A/en not_active Expired - Lifetime
-
1975
- 1975-06-23 CA CA229,927A patent/CA1049402A/en not_active Expired
- 1975-06-24 GB GB26681/75A patent/GB1492899A/en not_active Expired
- 1975-07-22 FR FR7522874A patent/FR2280076A1/fr active Granted
- 1975-07-22 DE DE2532779A patent/DE2532779C3/de not_active Expired
- 1975-07-22 JP JP8893775A patent/JPS5529385B2/ja not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| DE2532779A1 (de) | 1976-02-12 |
| JPS5529385B2 (ref) | 1980-08-02 |
| GB1492899A (en) | 1977-11-23 |
| DE2532779B2 (de) | 1980-09-25 |
| FR2280076A1 (fr) | 1976-02-20 |
| FR2280076B1 (ref) | 1979-05-04 |
| JPS5149090A (ref) | 1976-04-27 |
| US3912610A (en) | 1975-10-14 |
| CA1049402A (en) | 1979-02-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OD | Request for examination | ||
| C3 | Grant after two publication steps (3rd publication) | ||
| 8339 | Ceased/non-payment of the annual fee |