DE2517860C3 - Verfahren zum Bestimmen des Pyrogengehalts in Proben - Google Patents
Verfahren zum Bestimmen des Pyrogengehalts in ProbenInfo
- Publication number
- DE2517860C3 DE2517860C3 DE19752517860 DE2517860A DE2517860C3 DE 2517860 C3 DE2517860 C3 DE 2517860C3 DE 19752517860 DE19752517860 DE 19752517860 DE 2517860 A DE2517860 A DE 2517860A DE 2517860 C3 DE2517860 C3 DE 2517860C3
- Authority
- DE
- Germany
- Prior art keywords
- protein
- amount
- solution
- pyrogen
- precipitated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000002510 pyrogen Substances 0.000 title claims description 30
- 238000000034 method Methods 0.000 title claims description 22
- 239000000243 solution Substances 0.000 claims description 70
- 108090000623 proteins and genes Proteins 0.000 claims description 61
- 102000004169 proteins and genes Human genes 0.000 claims description 60
- 238000010790 dilution Methods 0.000 claims description 28
- 239000012895 dilution Substances 0.000 claims description 28
- 241000239218 Limulus Species 0.000 claims description 27
- 239000006166 lysate Substances 0.000 claims description 24
- 239000003153 chemical reaction reagent Substances 0.000 claims description 13
- 238000006243 chemical reaction Methods 0.000 claims description 11
- 238000010521 absorption reaction Methods 0.000 claims description 10
- 238000002798 spectrophotometry method Methods 0.000 claims description 7
- 239000006185 dispersion Substances 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 239000012460 protein solution Substances 0.000 claims description 6
- 230000031700 light absorption Effects 0.000 claims description 5
- 230000035945 sensitivity Effects 0.000 claims description 4
- 230000006920 protein precipitation Effects 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 238000001556 precipitation Methods 0.000 claims description 2
- 230000000052 comparative effect Effects 0.000 claims 1
- 238000011084 recovery Methods 0.000 claims 1
- 239000002158 endotoxin Substances 0.000 description 45
- 238000012360 testing method Methods 0.000 description 32
- 210000004369 blood Anatomy 0.000 description 15
- 239000008280 blood Substances 0.000 description 15
- 239000000523 sample Substances 0.000 description 11
- 210000004027 cell Anatomy 0.000 description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 238000002835 absorbance Methods 0.000 description 5
- 239000011148 porous material Substances 0.000 description 5
- 241001529572 Chaceon affinis Species 0.000 description 4
- 238000001914 filtration Methods 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 230000003287 optical effect Effects 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 210000000601 blood cell Anatomy 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 230000005484 gravity Effects 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 241000239220 Limulus polyphemus Species 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 208000007536 Thrombosis Diseases 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 239000010200 folin Substances 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000007911 parenteral administration Methods 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000012085 test solution Substances 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 1
- 231100000699 Bacterial toxin Toxicity 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- MJOQJPYNENPSSS-XQHKEYJVSA-N [(3r,4s,5r,6s)-4,5,6-triacetyloxyoxan-3-yl] acetate Chemical compound CC(=O)O[C@@H]1CO[C@@H](OC(C)=O)[C@H](OC(C)=O)[C@H]1OC(C)=O MJOQJPYNENPSSS-XQHKEYJVSA-N 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004378 air conditioning Methods 0.000 description 1
- 238000000149 argon plasma sintering Methods 0.000 description 1
- 239000000688 bacterial toxin Substances 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- 239000012496 blank sample Substances 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 239000012470 diluted sample Substances 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 229920006008 lipopolysaccharide Polymers 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- PSGAAPLEWMOORI-PEINSRQWSA-N medroxyprogesterone acetate Chemical compound C([C@@]12C)CC(=O)C=C1[C@@H](C)C[C@@H]1[C@@H]2CC[C@]2(C)[C@@](OC(C)=O)(C(C)=O)CC[C@H]21 PSGAAPLEWMOORI-PEINSRQWSA-N 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000011045 prefiltration Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000001698 pyrogenic effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- GNBVPFITFYNRCN-UHFFFAOYSA-M sodium thioglycolate Chemical compound [Na+].[O-]C(=O)CS GNBVPFITFYNRCN-UHFFFAOYSA-M 0.000 description 1
- 229940046307 sodium thioglycolate Drugs 0.000 description 1
- 239000010414 supernatant solution Substances 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/579—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving limulus lysate
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US46599074A | 1974-05-01 | 1974-05-01 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| DE2517860A1 DE2517860A1 (de) | 1975-11-13 |
| DE2517860B2 DE2517860B2 (de) | 1979-04-19 |
| DE2517860C3 true DE2517860C3 (de) | 1980-01-03 |
Family
ID=23849996
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19752517860 Expired DE2517860C3 (de) | 1974-05-01 | 1975-04-22 | Verfahren zum Bestimmen des Pyrogengehalts in Proben |
Country Status (6)
| Country | Link |
|---|---|
| JP (1) | JPS50147989A (oth) |
| AU (1) | AU497873B2 (oth) |
| CA (1) | CA1040081A (oth) |
| DE (1) | DE2517860C3 (oth) |
| FR (1) | FR2280083A1 (oth) |
| GB (1) | GB1499846A (oth) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3944391A (en) * | 1974-12-27 | 1976-03-16 | Preventive Systems, Inc. | In vitro process for detecting endotoxin in a biological fluid |
| US4221866A (en) | 1978-10-06 | 1980-09-09 | Baxter Travenol Laboratories, Inc. | Method for determining endotoxin concentration |
| US4221865A (en) | 1978-10-06 | 1980-09-09 | Baxter Travenol Laboratories, Inc. | Method for determining endotoxin concentration |
| US4376819A (en) | 1979-09-14 | 1983-03-15 | Baxter Travenol Laboratories, Inc. | Biological extracts and method for making same |
| ES2057128T3 (es) * | 1988-07-05 | 1994-10-16 | Wako Pure Chem Ind Ltd | Procedimiento para medir endotoxinas. |
-
1975
- 1975-04-11 CA CA224,426A patent/CA1040081A/en not_active Expired
- 1975-04-22 GB GB1650975A patent/GB1499846A/en not_active Expired
- 1975-04-22 DE DE19752517860 patent/DE2517860C3/de not_active Expired
- 1975-04-28 JP JP5124975A patent/JPS50147989A/ja active Pending
- 1975-04-28 FR FR7513235A patent/FR2280083A1/fr active Granted
- 1975-04-30 AU AU80691/75A patent/AU497873B2/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| FR2280083B1 (oth) | 1982-02-05 |
| FR2280083A1 (fr) | 1976-02-20 |
| DE2517860B2 (de) | 1979-04-19 |
| AU497873B2 (en) | 1979-01-18 |
| CA1040081A (en) | 1978-10-10 |
| DE2517860A1 (de) | 1975-11-13 |
| JPS50147989A (oth) | 1975-11-27 |
| GB1499846A (en) | 1978-02-01 |
| AU8069175A (en) | 1976-11-04 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C3 | Grant after two publication steps (3rd publication) | ||
| 8328 | Change in the person/name/address of the agent |
Free format text: KERN, R., DIPL.-ING., PAT.-ANW., 8000 MUENCHEN |
|
| 8328 | Change in the person/name/address of the agent |
Free format text: POPP, E., DIPL.-ING.DIPL.-WIRTSCH.-ING.DR.RER.POL. SAJDA, W., DIPL.-PHYS. VON BUELOW, T., DIPL.-ING.DIPL.-WIRTSCH.-ING.DR.RER.POL., 8000 MUENCHEN BOLTE, E., DIPL.-ING., 2800 BREMEN HRABAL, U., DIPL.-CHEM. DR.RER.NAT., PAT.-ANW., 8000 MUENCHEN |
|
| 8328 | Change in the person/name/address of the agent |
Free format text: POPP, E., DIPL.-ING.DIPL.-WIRTSCH.-ING.DR.RER.POL. SAJDA, W., DIPL.-PHYS. HRABAL, U., DIPL.-CHEM. DR.RER.NAT., 8000 MUENCHEN BOLTE, E., DIPL.-ING., PAT.-ANW., 2800 BREMEN |
|
| 8339 | Ceased/non-payment of the annual fee |