DE2512605B2 - Verfahren zur bestimmung des gesamt- cholesteringehaltes waessriger fluessigkeiten - Google Patents
Verfahren zur bestimmung des gesamt- cholesteringehaltes waessriger fluessigkeitenInfo
- Publication number
- DE2512605B2 DE2512605B2 DE19752512605 DE2512605A DE2512605B2 DE 2512605 B2 DE2512605 B2 DE 2512605B2 DE 19752512605 DE19752512605 DE 19752512605 DE 2512605 A DE2512605 A DE 2512605A DE 2512605 B2 DE2512605 B2 DE 2512605B2
- Authority
- DE
- Germany
- Prior art keywords
- lipase
- cholesterol
- protease
- serum
- activity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 title claims description 99
- 235000012000 cholesterol Nutrition 0.000 title claims description 47
- 238000000034 method Methods 0.000 title claims description 38
- 239000007788 liquid Substances 0.000 title claims description 14
- 102000004882 Lipase Human genes 0.000 claims description 58
- 108090001060 Lipase Proteins 0.000 claims description 58
- 239000004367 Lipase Substances 0.000 claims description 58
- 235000019421 lipase Nutrition 0.000 claims description 58
- 239000004365 Protease Substances 0.000 claims description 52
- 210000002966 serum Anatomy 0.000 claims description 40
- 108091005804 Peptidases Proteins 0.000 claims description 39
- 230000000694 effects Effects 0.000 claims description 28
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 27
- 235000019419 proteases Nutrition 0.000 claims description 27
- 150000001840 cholesterol esters Chemical class 0.000 claims description 21
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 12
- 102000035195 Peptidases Human genes 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 12
- 230000007062 hydrolysis Effects 0.000 claims description 11
- 238000006460 hydrolysis reaction Methods 0.000 claims description 11
- 108010055297 Sterol Esterase Proteins 0.000 claims description 9
- 102000000019 Sterol Esterase Human genes 0.000 claims description 9
- 240000006439 Aspergillus oryzae Species 0.000 claims description 6
- 108090000526 Papain Proteins 0.000 claims description 6
- 229910052757 nitrogen Inorganic materials 0.000 claims description 6
- 235000019834 papain Nutrition 0.000 claims description 6
- 229940055729 papain Drugs 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 5
- 108010004032 Bromelains Proteins 0.000 claims description 5
- 235000019835 bromelain Nutrition 0.000 claims description 5
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 4
- 235000002247 Aspergillus oryzae Nutrition 0.000 claims description 3
- 244000063299 Bacillus subtilis Species 0.000 claims description 3
- 241000222120 Candida <Saccharomycetales> Species 0.000 claims description 3
- 241000209140 Triticum Species 0.000 claims description 3
- 235000021307 Triticum Nutrition 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000009835 boiling Methods 0.000 claims description 2
- 244000005700 microbiome Species 0.000 claims description 2
- NRHMKIHPTBHXPF-TUJRSCDTSA-M sodium cholate Chemical compound [Na+].C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC([O-])=O)C)[C@@]2(C)[C@@H](O)C1 NRHMKIHPTBHXPF-TUJRSCDTSA-M 0.000 claims description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 claims 2
- 239000006185 dispersion Substances 0.000 claims 1
- 230000002906 microbiologic effect Effects 0.000 claims 1
- 239000008055 phosphate buffer solution Substances 0.000 claims 1
- 229960005322 streptomycin Drugs 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 description 18
- 108090000790 Enzymes Proteins 0.000 description 18
- 229940088598 enzyme Drugs 0.000 description 18
- 108090000371 Esterases Proteins 0.000 description 8
- IMNFDUFMRHMDMM-UHFFFAOYSA-N N-Heptane Chemical compound CCCCCCC IMNFDUFMRHMDMM-UHFFFAOYSA-N 0.000 description 6
- 229960002376 chymotrypsin Drugs 0.000 description 6
- 150000002148 esters Chemical class 0.000 description 6
- 239000000758 substrate Substances 0.000 description 6
- 108010089254 Cholesterol oxidase Proteins 0.000 description 5
- NAACPBBQTFFYQB-UHFFFAOYSA-N Linolsaeure-cholesterylester Natural products C12CCC3(C)C(C(C)CCCC(C)C)CCC3C2CC=C2C1(C)CCC(OC(=O)CCCCCCCC=CCC=CCCCCC)C2 NAACPBBQTFFYQB-UHFFFAOYSA-N 0.000 description 5
- 239000012928 buffer substance Substances 0.000 description 5
- NAACPBBQTFFYQB-XNTGVSEISA-N cholesteryl octadeca-9,12-dienoate Chemical compound C([C@@H]12)C[C@]3(C)[C@@H]([C@H](C)CCCC(C)C)CC[C@H]3[C@@H]1CC=C1[C@]2(C)CC[C@H](OC(=O)CCCCCCCC=CCC=CCCCCC)C1 NAACPBBQTFFYQB-XNTGVSEISA-N 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 238000001030 gas--liquid chromatography Methods 0.000 description 5
- ZYURHZPYMFLWSH-UHFFFAOYSA-N octacosane Chemical compound CCCCCCCCCCCCCCCCCCCCCCCCCCCC ZYURHZPYMFLWSH-UHFFFAOYSA-N 0.000 description 5
- 239000000872 buffer Substances 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 4
- 102000004895 Lipoproteins Human genes 0.000 description 3
- 108090001030 Lipoproteins Proteins 0.000 description 3
- 108010059712 Pronase Proteins 0.000 description 3
- 241000187392 Streptomyces griseus Species 0.000 description 3
- 239000012736 aqueous medium Substances 0.000 description 3
- 239000007853 buffer solution Substances 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 230000002255 enzymatic effect Effects 0.000 description 3
- 238000007254 oxidation reaction Methods 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 108090000787 Subtilisin Proteins 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 239000012298 atmosphere Substances 0.000 description 2
- IJOOHPMOJXWVHK-UHFFFAOYSA-N chlorotrimethylsilane Chemical compound C[Si](C)(C)Cl IJOOHPMOJXWVHK-UHFFFAOYSA-N 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 239000000839 emulsion Substances 0.000 description 2
- 238000006911 enzymatic reaction Methods 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 108091016642 steapsin Proteins 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- NEAQRZUHTPSBBM-UHFFFAOYSA-N 2-hydroxy-3,3-dimethyl-7-nitro-4h-isoquinolin-1-one Chemical class C1=C([N+]([O-])=O)C=C2C(=O)N(O)C(C)(C)CC2=C1 NEAQRZUHTPSBBM-UHFFFAOYSA-N 0.000 description 1
- 108010015598 Chromobacterium viscosum lipase Proteins 0.000 description 1
- 108090000317 Chymotrypsin Proteins 0.000 description 1
- AVXURJPOCDRRFD-UHFFFAOYSA-N Hydroxylamine Chemical compound ON AVXURJPOCDRRFD-UHFFFAOYSA-N 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 235000000434 Melocanna baccifera Nutrition 0.000 description 1
- 241001497770 Melocanna baccifera Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- BAVYZALUXZFZLV-UHFFFAOYSA-N Methylamine Chemical compound NC BAVYZALUXZFZLV-UHFFFAOYSA-N 0.000 description 1
- 108010067372 Pancreatic elastase Proteins 0.000 description 1
- 102000016387 Pancreatic elastase Human genes 0.000 description 1
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 1
- 241000303962 Rhizopus delemar Species 0.000 description 1
- 108090001109 Thermolysin Proteins 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- GGCLNOIGPMGLDB-GYKMGIIDSA-N cholest-5-en-3-one Chemical compound C1C=C2CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 GGCLNOIGPMGLDB-GYKMGIIDSA-N 0.000 description 1
- NYOXRYYXRWJDKP-UHFFFAOYSA-N cholestenone Natural products C1CC2=CC(=O)CCC2(C)C2C1C1CCC(C(C)CCCC(C)C)C1(C)CC2 NYOXRYYXRWJDKP-UHFFFAOYSA-N 0.000 description 1
- 150000001841 cholesterols Chemical class 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010931 ester hydrolysis Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- IPCSVZSSVZVIGE-UHFFFAOYSA-M hexadecanoate Chemical compound CCCCCCCCCCCCCCCC([O-])=O IPCSVZSSVZVIGE-UHFFFAOYSA-M 0.000 description 1
- 150000003840 hydrochlorides Chemical class 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- IZXGZAJMDLJLMF-UHFFFAOYSA-N methylaminomethanol Chemical compound CNCO IZXGZAJMDLJLMF-UHFFFAOYSA-N 0.000 description 1
- 239000012299 nitrogen atmosphere Substances 0.000 description 1
- 229940049964 oleate Drugs 0.000 description 1
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 1
- 239000004006 olive oil Substances 0.000 description 1
- 235000008390 olive oil Nutrition 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 230000036284 oxygen consumption Effects 0.000 description 1
- UOJMTSCORVQOHS-UHFFFAOYSA-N pachypodol Natural products COc1cc(ccc1O)C2=C(C)C(=O)c3c(O)cc(C)cc3O2 UOJMTSCORVQOHS-UHFFFAOYSA-N 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000007127 saponification reaction Methods 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 229920005573 silicon-containing polymer Polymers 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 150000003626 triacylglycerols Chemical class 0.000 description 1
- 239000005051 trimethylchlorosilane Substances 0.000 description 1
- 125000000026 trimethylsilyl group Chemical group [H]C([H])([H])[Si]([*])(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/832—Bacillus
- Y10S435/839—Bacillus subtilis
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/886—Streptomyces
- Y10S435/897—Streptomyces griseus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/911—Microorganisms using fungi
- Y10S435/913—Aspergillus
- Y10S435/918—Aspergillus oryzae
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/911—Microorganisms using fungi
- Y10S435/921—Candida
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US454659A US3869349A (en) | 1974-03-25 | 1974-03-25 | Method for the enzymatic hydrolysis of cholesterol esters |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE2512605A1 DE2512605A1 (de) | 1975-10-02 |
| DE2512605B2 true DE2512605B2 (de) | 1976-11-18 |
Family
ID=23805542
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19752512605 Withdrawn DE2512605B2 (de) | 1974-03-25 | 1975-03-21 | Verfahren zur bestimmung des gesamt- cholesteringehaltes waessriger fluessigkeiten |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US3869349A (enExample) |
| JP (1) | JPS5615240B2 (enExample) |
| CA (1) | CA1046389A (enExample) |
| DE (1) | DE2512605B2 (enExample) |
| FR (1) | FR2265759B1 (enExample) |
| GB (1) | GB1498461A (enExample) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS50157588A (enExample) * | 1974-06-17 | 1975-12-19 | ||
| US4011045A (en) * | 1975-02-14 | 1977-03-08 | Bonderman Dean P | Turbidity reduction in triglyceride standards |
| US4275152A (en) * | 1977-02-03 | 1981-06-23 | Eastman Kodak Company | Hydrolysis of protein-bound cholesterol esters |
| US4275151A (en) * | 1977-02-03 | 1981-06-23 | Eastman Kodak Company | Hydrolysis of protein-bound cholesterol esters |
| US4259440A (en) * | 1979-05-21 | 1981-03-31 | Miles Laboratories, Inc. | Hydrolysis and assay of triglycerides |
| US4680259A (en) * | 1984-09-26 | 1987-07-14 | Eastman Kodak Company | Analytical element and method for colorimetric determination of total cholesterol |
| US5219733A (en) * | 1985-03-06 | 1993-06-15 | Yoshikawa Oil & Fat Co., Ltd. | Process for preparing fatty acid esters |
| US5116741A (en) * | 1988-04-12 | 1992-05-26 | Genex Corporation | Biosynthetic uses of thermostable proteases |
| CN112877219A (zh) * | 2021-01-29 | 2021-06-01 | 江西科技师范大学 | 一种高浓度胆固醇培养基及其制备方法和应用 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2224132C2 (de) * | 1972-05-17 | 1980-04-03 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren und Reagenz zur Bestimmung von Cholesterin |
-
1974
- 1974-03-25 US US454659A patent/US3869349A/en not_active Expired - Lifetime
-
1975
- 1975-02-19 CA CA220,426A patent/CA1046389A/en not_active Expired
- 1975-03-21 DE DE19752512605 patent/DE2512605B2/de not_active Withdrawn
- 1975-03-24 GB GB12202/75A patent/GB1498461A/en not_active Expired
- 1975-03-25 FR FR7509199A patent/FR2265759B1/fr not_active Expired
- 1975-03-25 JP JP3504475A patent/JPS5615240B2/ja not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| GB1498461A (en) | 1978-01-18 |
| CA1046389A (en) | 1979-01-16 |
| JPS50131588A (enExample) | 1975-10-17 |
| FR2265759B1 (enExample) | 1977-11-25 |
| US3869349A (en) | 1975-03-04 |
| DE2512605A1 (de) | 1975-10-02 |
| JPS5615240B2 (enExample) | 1981-04-09 |
| FR2265759A1 (enExample) | 1975-10-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP0265933B1 (de) | Verfahren und Reagenz zur spezifischen Bestimmung des Cholesterins der HDL-Fraktion | |
| DE69610939T2 (de) | Verfahren zur Interesterifikation von Phospholipiden | |
| DE2409696A1 (de) | Mittel und verfahren zur bestimmung von cholesterin | |
| DE69219530T2 (de) | Verfahren zur Herstellung von R(-)-Mandelsäure und deren Derivat | |
| Abousalham et al. | Egg yolk lipoproteins as substrates for lipases | |
| DE2512605A1 (de) | Verfahren zur bestimmung des gesamt-cholesteringehaltes waessriger fluessigkeiten | |
| DE2653537A1 (de) | Verfahren und mittel zur bestimmung von hydroperoxiden | |
| DE3881091T2 (de) | Verfahren zur herstellung von phosphatidsaeurederivaten. | |
| CH620472A5 (enExample) | ||
| DE68924738T2 (de) | Hitzestabile Lipoproteinlipase, Verfahren zu deren Herstellung sowie ein Reagenz zur Bestimmung von Triglyceriden, welches diese Lipase enthält. | |
| DE2737288C2 (de) | Verfahren zur Bestimmung des freien und/oder in Form von Triglyceriden vorliegenden gebundenen Glycerins in wäßrigen Flüssigkeiten sowie Bestimmungsreagenz für die Durchführung des Verfahrens und Bestimmungsreagenz für die Bestimmung von Adenosintriphosphat | |
| EP0863995B1 (de) | Verbessertes verfahren zur bestimmung von lipase | |
| EP0084684B1 (de) | Verfahren zur Stabilisierung wässriger Lösungen von Cholesterinesterase aus Pseudomonaden | |
| EP0144798B1 (de) | Verfahren und Reagenz zur Bestimmung von Glycerin mittels eines kompetitiven Inhibitors für Glycerinkinase | |
| EP0048347A1 (de) | Verfahren und Reagens zur Bestimmung von Glycerin | |
| CH657919A5 (de) | Reagens zur beseitigung einer truebung in einer biologischen fluessigkeit. | |
| DE19651886A1 (de) | Mittel und Verfahren zur Bestimmung von hydrolytischen Enzymen | |
| DE2506712C3 (de) | Verfahren zur Bestimmung von Cholesterin | |
| DE69008733T2 (de) | Interesterifikation von phospholipiden. | |
| DE69329679T2 (de) | Regioselektives verfahren zur racematspaltung von fettsäuremonoestersacchariden | |
| DE3301655A1 (de) | Verfahren zur quantitativen bestimmung von ungesaettigten fettsaeuren | |
| DE1815845C3 (de) | Verfahren zur biochemischen Abtrennung von 1-Menthol | |
| EP0476669B1 (de) | Verfahren zur Bestimmung von Phospholipase A in Körperflüssigkeiten | |
| DE19733309A1 (de) | Verbessertes Verfahren zur Bestimmung von Lipase | |
| DE4136844A1 (de) | Arthrobacter-nicotiana-stamm, aktivitaet des stamms, verfahren zur isolierung des stamms, sensor mit der aktiviaet und verwendung des sensors |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8230 | Patent withdrawn |