GB1498461A - Quantitative determination of cholesterol - Google Patents

Quantitative determination of cholesterol

Info

Publication number
GB1498461A
GB1498461A GB12202/75A GB1220275A GB1498461A GB 1498461 A GB1498461 A GB 1498461A GB 12202/75 A GB12202/75 A GB 12202/75A GB 1220275 A GB1220275 A GB 1220275A GB 1498461 A GB1498461 A GB 1498461A
Authority
GB
United Kingdom
Prior art keywords
cholesterol
lipase
aqueous liquid
march
units
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB12202/75A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eastman Kodak Co
Original Assignee
Eastman Kodak Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eastman Kodak Co filed Critical Eastman Kodak Co
Publication of GB1498461A publication Critical patent/GB1498461A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/832—Bacillus
    • Y10S435/839—Bacillus subtilis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/886—Streptomyces
    • Y10S435/897—Streptomyces griseus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/913—Aspergillus
    • Y10S435/918—Aspergillus oryzae
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/921—Candida

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

1498461 Enzymolysis of cholesteryl esters EASTMAN KODAK CO 24 March 1975 [25 March 1974] 12202/75 Heading C2U [Also in Division G1] Cholesterol esters in an aqueous liquid are hydrolysed to liberate cholesterol by treating the aqueous liquid with both a protease and a lipase having cholesterol esterase activity (defined as r lipase which releases cholesterol from cholesterol linoleate when incubated with this under specified conditions). The aqueous liquid may be blood serum may be treated with 600 to 1500 units (20 to 50 mg.) of the lipase and 50 to 500 units (5 to 50 mg.) of the protease per ml at a temperature of from 25‹ to 55‹ C. and a pH of from 6À5 to 9À5, and the cholesterol released may be determined by gas chromatography. The lipase may be of plant or animal origin but is preferably a microbial lipase.
GB12202/75A 1974-03-25 1975-03-24 Quantitative determination of cholesterol Expired GB1498461A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US454659A US3869349A (en) 1974-03-25 1974-03-25 Method for the enzymatic hydrolysis of cholesterol esters

Publications (1)

Publication Number Publication Date
GB1498461A true GB1498461A (en) 1978-01-18

Family

ID=23805542

Family Applications (1)

Application Number Title Priority Date Filing Date
GB12202/75A Expired GB1498461A (en) 1974-03-25 1975-03-24 Quantitative determination of cholesterol

Country Status (6)

Country Link
US (1) US3869349A (en)
JP (1) JPS5615240B2 (en)
CA (1) CA1046389A (en)
DE (1) DE2512605B2 (en)
FR (1) FR2265759B1 (en)
GB (1) GB1498461A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112877219A (en) * 2021-01-29 2021-06-01 江西科技师范大学 High-concentration cholesterol culture medium and preparation method and application thereof

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS50157588A (en) * 1974-06-17 1975-12-19
US4011045A (en) * 1975-02-14 1977-03-08 Bonderman Dean P Turbidity reduction in triglyceride standards
US4275152A (en) * 1977-02-03 1981-06-23 Eastman Kodak Company Hydrolysis of protein-bound cholesterol esters
US4275151A (en) * 1977-02-03 1981-06-23 Eastman Kodak Company Hydrolysis of protein-bound cholesterol esters
US4259440A (en) * 1979-05-21 1981-03-31 Miles Laboratories, Inc. Hydrolysis and assay of triglycerides
US4680259A (en) * 1984-09-26 1987-07-14 Eastman Kodak Company Analytical element and method for colorimetric determination of total cholesterol
US5219733A (en) * 1985-03-06 1993-06-15 Yoshikawa Oil & Fat Co., Ltd. Process for preparing fatty acid esters
US5116741A (en) * 1988-04-12 1992-05-26 Genex Corporation Biosynthetic uses of thermostable proteases

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2264847C2 (en) * 1972-05-17 1978-11-30 Boehringer Mannheim Gmbh, 6800 Mannheim Method and reagent for the determination of cholesterol

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112877219A (en) * 2021-01-29 2021-06-01 江西科技师范大学 High-concentration cholesterol culture medium and preparation method and application thereof

Also Published As

Publication number Publication date
JPS5615240B2 (en) 1981-04-09
DE2512605A1 (en) 1975-10-02
FR2265759A1 (en) 1975-10-24
JPS50131588A (en) 1975-10-17
US3869349A (en) 1975-03-04
DE2512605B2 (en) 1976-11-18
FR2265759B1 (en) 1977-11-25
CA1046389A (en) 1979-01-16

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PCNP Patent ceased through non-payment of renewal fee