DE2426519C2 - Strahlung aussendende Markierungssubstanz, Verfahren zu ihrer Herstellung sowie sie enthaltendes markiertes Reagens - Google Patents
Strahlung aussendende Markierungssubstanz, Verfahren zu ihrer Herstellung sowie sie enthaltendes markiertes ReagensInfo
- Publication number
- DE2426519C2 DE2426519C2 DE19742426519 DE2426519A DE2426519C2 DE 2426519 C2 DE2426519 C2 DE 2426519C2 DE 19742426519 DE19742426519 DE 19742426519 DE 2426519 A DE2426519 A DE 2426519A DE 2426519 C2 DE2426519 C2 DE 2426519C2
- Authority
- DE
- Germany
- Prior art keywords
- radiation
- reactive
- labeled reagent
- reactant
- antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000000126 substance Substances 0.000 title claims description 31
- 239000003153 chemical reaction reagent Substances 0.000 title claims description 15
- 238000000034 method Methods 0.000 title description 10
- 230000008569 process Effects 0.000 title description 5
- 238000002372 labelling Methods 0.000 title description 4
- 238000002360 preparation method Methods 0.000 title description 3
- 239000000376 reactant Substances 0.000 claims description 21
- 238000009739 binding Methods 0.000 claims description 8
- 230000009870 specific binding Effects 0.000 claims description 4
- 229920000642 polymer Polymers 0.000 description 22
- 230000005855 radiation Effects 0.000 description 22
- 238000006243 chemical reaction Methods 0.000 description 18
- 239000000975 dye Substances 0.000 description 15
- 241000700605 Viruses Species 0.000 description 8
- 238000001514 detection method Methods 0.000 description 7
- 108090000623 proteins and genes Proteins 0.000 description 7
- 102000004169 proteins and genes Human genes 0.000 description 7
- 230000035945 sensitivity Effects 0.000 description 7
- BAVYZALUXZFZLV-UHFFFAOYSA-N Methylamine Chemical compound NC BAVYZALUXZFZLV-UHFFFAOYSA-N 0.000 description 6
- 239000000427 antigen Substances 0.000 description 6
- 102000036639 antigens Human genes 0.000 description 6
- 108091007433 antigens Proteins 0.000 description 6
- 230000027455 binding Effects 0.000 description 6
- 230000000903 blocking effect Effects 0.000 description 6
- 239000002245 particle Substances 0.000 description 6
- 239000007795 chemical reaction product Substances 0.000 description 5
- HUMNYLRZRPPJDN-UHFFFAOYSA-N benzaldehyde Chemical compound O=CC1=CC=CC=C1 HUMNYLRZRPPJDN-UHFFFAOYSA-N 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 230000000984 immunochemical effect Effects 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 230000009257 reactivity Effects 0.000 description 4
- 229920002873 Polyethylenimine Polymers 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 230000007062 hydrolysis Effects 0.000 description 3
- 238000006460 hydrolysis reaction Methods 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 108060003951 Immunoglobulin Proteins 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 102000018358 immunoglobulin Human genes 0.000 description 2
- 239000000543 intermediate Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000178 monomer Substances 0.000 description 2
- QNGNSVIICDLXHT-UHFFFAOYSA-N para-ethylbenzaldehyde Natural products CCC1=CC=C(C=O)C=C1 QNGNSVIICDLXHT-UHFFFAOYSA-N 0.000 description 2
- 230000003252 repetitive effect Effects 0.000 description 2
- 101710098119 Chaperonin GroEL 2 Proteins 0.000 description 1
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 description 1
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 1
- 239000004952 Polyamide Substances 0.000 description 1
- ZMZDMBWJUHKJPS-UHFFFAOYSA-M Thiocyanate anion Chemical compound [S-]C#N ZMZDMBWJUHKJPS-UHFFFAOYSA-M 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000000010 aprotic solvent Substances 0.000 description 1
- 239000012736 aqueous medium Substances 0.000 description 1
- 150000001735 carboxylic acids Chemical class 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 239000000539 dimer Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229920001109 fluorescent polymer Polymers 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- ZMZDMBWJUHKJPS-UHFFFAOYSA-N hydrogen thiocyanate Natural products SC#N ZMZDMBWJUHKJPS-UHFFFAOYSA-N 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229910052744 lithium Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 125000000896 monocarboxylic acid group Chemical group 0.000 description 1
- 238000005375 photometry Methods 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- ZWZVWGITAAIFPS-UHFFFAOYSA-N thiophosgene Chemical compound ClC(Cl)=S ZWZVWGITAAIFPS-UHFFFAOYSA-N 0.000 description 1
- 239000013638 trimer Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US38389273A | 1973-07-30 | 1973-07-30 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE2426519A1 DE2426519A1 (de) | 1975-02-20 |
| DE2426519C2 true DE2426519C2 (de) | 1985-04-18 |
Family
ID=23515170
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19742426519 Expired DE2426519C2 (de) | 1973-07-30 | 1974-05-31 | Strahlung aussendende Markierungssubstanz, Verfahren zu ihrer Herstellung sowie sie enthaltendes markiertes Reagens |
Country Status (7)
| Country | Link |
|---|---|
| JP (1) | JPS5045692A (enExample) |
| CA (1) | CA1041422A (enExample) |
| CH (1) | CH607022A5 (enExample) |
| DE (1) | DE2426519C2 (enExample) |
| FR (1) | FR2245948A1 (enExample) |
| GB (1) | GB1470074A (enExample) |
| NL (1) | NL7410241A (enExample) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4434150A (en) * | 1981-10-19 | 1984-02-28 | Ortho Diagnostic Systems, Inc. | Immunological reagents employing polymeric backbone possessing reactive functional groups |
| US4687732A (en) * | 1983-06-10 | 1987-08-18 | Yale University | Visualization polymers and their application to diagnostic medicine |
| US4500788A (en) * | 1983-08-19 | 1985-02-19 | Baxter Travenol Laboratories, Inc. | Device for providing antibacterial radiation |
| JP4848988B2 (ja) * | 2007-03-26 | 2011-12-28 | セイコーエプソン株式会社 | 検出キット |
| JP5071575B2 (ja) * | 2011-07-29 | 2012-11-14 | セイコーエプソン株式会社 | 検出キット |
-
1974
- 1974-05-22 GB GB2279174A patent/GB1470074A/en not_active Expired
- 1974-05-23 CA CA200,645A patent/CA1041422A/en not_active Expired
- 1974-05-31 DE DE19742426519 patent/DE2426519C2/de not_active Expired
- 1974-07-17 FR FR7424889A patent/FR2245948A1/fr not_active Withdrawn
- 1974-07-23 CH CH1016974A patent/CH607022A5/xx not_active IP Right Cessation
- 1974-07-24 JP JP8504674A patent/JPS5045692A/ja active Pending
- 1974-07-30 NL NL7410241A patent/NL7410241A/xx not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5045692A (enExample) | 1975-04-23 |
| AU6954374A (en) | 1975-12-04 |
| GB1470074A (en) | 1977-04-14 |
| CH607022A5 (enExample) | 1978-11-30 |
| FR2245948A1 (enExample) | 1975-04-25 |
| NL7410241A (nl) | 1975-02-03 |
| DE2426519A1 (de) | 1975-02-20 |
| CA1041422A (en) | 1978-10-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8110 | Request for examination paragraph 44 | ||
| AG | Has addition no. |
Ref country code: DE Ref document number: 2557419 Format of ref document f/p: P |
|
| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee | ||
| AG | Has addition no. |
Ref country code: DE Ref document number: 2557419 Format of ref document f/p: P |