DE2418978A1 - Steroid-delta-isomerase - Google Patents
Steroid-delta-isomeraseInfo
- Publication number
- DE2418978A1 DE2418978A1 DE2418978A DE2418978A DE2418978A1 DE 2418978 A1 DE2418978 A1 DE 2418978A1 DE 2418978 A DE2418978 A DE 2418978A DE 2418978 A DE2418978 A DE 2418978A DE 2418978 A1 DE2418978 A1 DE 2418978A1
- Authority
- DE
- Germany
- Prior art keywords
- enzyme
- steroid
- isomerase
- buffer solution
- compared
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 102100039081 Steroid Delta-isomerase Human genes 0.000 title description 2
- 108010085346 steroid delta-isomerase Proteins 0.000 title description 2
- 102000004190 Enzymes Human genes 0.000 claims description 31
- 108090000790 Enzymes Proteins 0.000 claims description 31
- 239000008363 phosphate buffer Substances 0.000 claims description 16
- 239000004094 surface-active agent Substances 0.000 claims description 15
- 108090000769 Isomerases Proteins 0.000 claims description 13
- 102000004195 Isomerases Human genes 0.000 claims description 13
- 239000002244 precipitate Substances 0.000 claims description 12
- 150000003431 steroids Chemical class 0.000 claims description 12
- 239000007853 buffer solution Substances 0.000 claims description 11
- 244000005700 microbiome Species 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 10
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 9
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 9
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 9
- 150000001450 anions Chemical class 0.000 claims description 8
- 230000000694 effects Effects 0.000 claims description 6
- 210000002421 cell wall Anatomy 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 239000006228 supernatant Substances 0.000 claims description 5
- KTUQUZJOVNIKNZ-UHFFFAOYSA-N butan-1-ol;hydrate Chemical compound O.CCCCO KTUQUZJOVNIKNZ-UHFFFAOYSA-N 0.000 claims description 4
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 239000002736 nonionic surfactant Substances 0.000 claims description 4
- 239000013543 active substance Substances 0.000 claims description 3
- 230000006378 damage Effects 0.000 claims description 3
- OSVMTWJCGUFAOD-KZQROQTASA-N formestane Chemical compound O=C1CC[C@]2(C)[C@H]3CC[C@](C)(C(CC4)=O)[C@@H]4[C@@H]3CCC2=C1O OSVMTWJCGUFAOD-KZQROQTASA-N 0.000 claims description 3
- 238000010521 absorption reaction Methods 0.000 claims description 2
- 238000010626 work up procedure Methods 0.000 claims description 2
- 238000005259 measurement Methods 0.000 claims 1
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 6
- 230000029087 digestion Effects 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 238000010828 elution Methods 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- RQNOFUZGXHSHOT-UHFFFAOYSA-N 1-(diethylamino)ethanol Chemical group CCN(CC)C(C)O RQNOFUZGXHSHOT-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 241000187654 Nocardia Species 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 229920002307 Dextran Polymers 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 2
- 230000008033 biological extinction Effects 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 239000003599 detergent Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000008057 potassium phosphate buffer Substances 0.000 description 2
- 230000008707 rearrangement Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241001251094 Formica Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- HSRJKNPTNIJEKV-UHFFFAOYSA-N Guaifenesin Chemical compound COC1=CC=CC=C1OCC(O)CO HSRJKNPTNIJEKV-UHFFFAOYSA-N 0.000 description 1
- BAVYZALUXZFZLV-UHFFFAOYSA-N Methylamine Chemical compound NC BAVYZALUXZFZLV-UHFFFAOYSA-N 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 125000002877 alkyl aryl group Chemical group 0.000 description 1
- 238000005349 anion exchange Methods 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 239000000337 buffer salt Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- GGCLNOIGPMGLDB-GYKMGIIDSA-N cholest-5-en-3-one Chemical compound C1C=C2CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 GGCLNOIGPMGLDB-GYKMGIIDSA-N 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 150000002334 glycols Chemical class 0.000 description 1
- 125000004029 hydroxymethyl group Chemical group [H]OC([H])([H])* 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002808 molecular sieve Substances 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- -1 polyethylene Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229920001451 polypropylene glycol Polymers 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- MNRHZPCIEGLWGK-LEKSSAKUSA-N pregn-5-ene-3,20-dione Chemical compound C1C=C2CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 MNRHZPCIEGLWGK-LEKSSAKUSA-N 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/814—Enzyme separation or purification
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/814—Enzyme separation or purification
- Y10S435/815—Enzyme separation or purification by sorption
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/872—Nocardia
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Priority Applications (11)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE2418978A DE2418978A1 (de) | 1974-04-19 | 1974-04-19 | Steroid-delta-isomerase |
| AT138575A AT338201B (de) | 1974-04-19 | 1975-02-24 | Verfahren zur gewinnung von neuer steroid-delta-isomerase |
| IT21254/75A IT1034269B (it) | 1974-04-19 | 1975-03-13 | Steroide delta isomerasi |
| FI750749A FI53576C (fi) | 1974-04-19 | 1975-03-14 | Foerfarande foer framstaellning av steroid- -isomeras |
| NL7503467A NL7503467A (nl) | 1974-04-19 | 1975-03-24 | Steroide-delta-isomerase en werkwijze voor de bereiding daarvan. |
| FR7509530A FR2268023B1 (enExample) | 1974-04-19 | 1975-03-26 | |
| US05/566,481 US3948728A (en) | 1974-04-19 | 1975-04-09 | Steroid-delta-isomerase |
| GB1520875A GB1459731A (en) | 1974-04-19 | 1975-04-14 | Steroid delta-isomerase |
| CH492375A CH611315A5 (enExample) | 1974-04-19 | 1975-04-17 | |
| JP50047354A JPS50148583A (enExample) | 1974-04-19 | 1975-04-18 | |
| AT95176A AT355843B (de) | 1974-04-19 | 1976-02-11 | Verfahren zur bestimmung von 3-keto-delta 5 - steroiden |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE2418978A DE2418978A1 (de) | 1974-04-19 | 1974-04-19 | Steroid-delta-isomerase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2418978A1 true DE2418978A1 (de) | 1975-10-30 |
Family
ID=5913370
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE2418978A Withdrawn DE2418978A1 (de) | 1974-04-19 | 1974-04-19 | Steroid-delta-isomerase |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US3948728A (enExample) |
| JP (1) | JPS50148583A (enExample) |
| AT (1) | AT338201B (enExample) |
| CH (1) | CH611315A5 (enExample) |
| DE (1) | DE2418978A1 (enExample) |
| FI (1) | FI53576C (enExample) |
| FR (1) | FR2268023B1 (enExample) |
| GB (1) | GB1459731A (enExample) |
| IT (1) | IT1034269B (enExample) |
| NL (1) | NL7503467A (enExample) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2432713A1 (fr) * | 1978-08-02 | 1980-02-29 | Inst Nat Sante Rech Med | Application de la d5,3-ceto steroide isomerase dans les dosages immunoenzymatiques |
| FR2459479B1 (fr) * | 1979-06-21 | 1983-07-08 | Inst Nat Sante Rech Med | Procede de separation d'une substance proteinique a partir d'une solution la contenant par filtration d'affinite et application dudit procede aux dosages enzymatiques |
| US4250263A (en) * | 1979-11-13 | 1981-02-10 | Uop Inc. | Method of purification of thermally stable enzymes |
| JPS56109586A (en) * | 1980-02-05 | 1981-08-31 | Cpc International Inc | Production of fixed glucoseisomerase |
| WO2022032178A1 (en) | 2020-08-06 | 2022-02-10 | Mate Precision Technologies Inc. | Vise assembly |
| WO2022032148A1 (en) | 2020-08-06 | 2022-02-10 | Mate Precision Technologies Inc. | Tooling base assembly |
-
1974
- 1974-04-19 DE DE2418978A patent/DE2418978A1/de not_active Withdrawn
-
1975
- 1975-02-24 AT AT138575A patent/AT338201B/de not_active IP Right Cessation
- 1975-03-13 IT IT21254/75A patent/IT1034269B/it active
- 1975-03-14 FI FI750749A patent/FI53576C/fi not_active IP Right Cessation
- 1975-03-24 NL NL7503467A patent/NL7503467A/xx not_active Application Discontinuation
- 1975-03-26 FR FR7509530A patent/FR2268023B1/fr not_active Expired
- 1975-04-09 US US05/566,481 patent/US3948728A/en not_active Expired - Lifetime
- 1975-04-14 GB GB1520875A patent/GB1459731A/en not_active Expired
- 1975-04-17 CH CH492375A patent/CH611315A5/xx not_active IP Right Cessation
- 1975-04-18 JP JP50047354A patent/JPS50148583A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| NL7503467A (nl) | 1975-10-21 |
| FI53576B (enExample) | 1978-02-28 |
| FR2268023A1 (enExample) | 1975-11-14 |
| FR2268023B1 (enExample) | 1978-06-30 |
| FI53576C (fi) | 1978-06-12 |
| GB1459731A (en) | 1976-12-31 |
| US3948728A (en) | 1976-04-06 |
| CH611315A5 (enExample) | 1979-05-31 |
| FI750749A7 (enExample) | 1975-10-20 |
| IT1034269B (it) | 1979-09-10 |
| ATA138575A (de) | 1976-12-15 |
| AT338201B (de) | 1977-08-10 |
| JPS50148583A (enExample) | 1975-11-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8141 | Disposal/no request for examination |