DE2350691C2 - Herstellung von Pankreas-Elastase - Google Patents
Herstellung von Pankreas-ElastaseInfo
- Publication number
- DE2350691C2 DE2350691C2 DE2350691A DE2350691A DE2350691C2 DE 2350691 C2 DE2350691 C2 DE 2350691C2 DE 2350691 A DE2350691 A DE 2350691A DE 2350691 A DE2350691 A DE 2350691A DE 2350691 C2 DE2350691 C2 DE 2350691C2
- Authority
- DE
- Germany
- Prior art keywords
- elastase
- added
- buffer solution
- hours
- precipitate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 102000016387 Pancreatic elastase Human genes 0.000 title claims description 58
- 108010067372 Pancreatic elastase Proteins 0.000 title claims description 58
- 238000004519 manufacturing process Methods 0.000 title claims description 7
- 238000000034 method Methods 0.000 claims description 34
- 239000002244 precipitate Substances 0.000 claims description 31
- 238000011534 incubation Methods 0.000 claims description 28
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 24
- 239000000243 solution Substances 0.000 claims description 24
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 23
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 23
- 210000000496 pancreas Anatomy 0.000 claims description 13
- 238000005185 salting out Methods 0.000 claims description 8
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims 1
- 239000013078 crystal Substances 0.000 description 25
- 239000000203 mixture Substances 0.000 description 24
- 239000007853 buffer solution Substances 0.000 description 22
- 238000001914 filtration Methods 0.000 description 22
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 14
- 238000002425 crystallisation Methods 0.000 description 13
- 230000008025 crystallization Effects 0.000 description 13
- 150000003839 salts Chemical class 0.000 description 12
- 239000008351 acetate buffer Substances 0.000 description 11
- 239000000706 filtrate Substances 0.000 description 9
- 239000000463 material Substances 0.000 description 9
- 239000010802 sludge Substances 0.000 description 9
- 238000001816 cooling Methods 0.000 description 8
- 230000000052 comparative effect Effects 0.000 description 7
- 230000000694 effects Effects 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 7
- 238000000502 dialysis Methods 0.000 description 6
- 108010019160 Pancreatin Proteins 0.000 description 5
- 229940055695 pancreatin Drugs 0.000 description 5
- 239000008055 phosphate buffer solution Substances 0.000 description 5
- 102000005686 Serum Globulins Human genes 0.000 description 4
- 108010045362 Serum Globulins Proteins 0.000 description 4
- 102000016942 Elastin Human genes 0.000 description 3
- 108010014258 Elastin Proteins 0.000 description 3
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 230000004913 activation Effects 0.000 description 3
- 239000001166 ammonium sulphate Substances 0.000 description 3
- 229920002549 elastin Polymers 0.000 description 3
- 239000012535 impurity Substances 0.000 description 3
- 235000013372 meat Nutrition 0.000 description 3
- 210000001819 pancreatic juice Anatomy 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000007858 starting material Substances 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 102000035195 Peptidases Human genes 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-M Aminoacetate Chemical compound NCC([O-])=O DHMQDGOQFOQNFH-UHFFFAOYSA-M 0.000 description 1
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000003125 aqueous solvent Substances 0.000 description 1
- 239000007900 aqueous suspension Substances 0.000 description 1
- 229920002301 cellulose acetate Polymers 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 235000015277 pork Nutrition 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 235000002639 sodium chloride Nutrition 0.000 description 1
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000010414 supernatant solution Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6448—Elastases, e.g. pancreatic elastase (3.4.21.36); leukocyte elastase (3.4.31.37)
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP47100656A JPS5021557B2 (en:Method) | 1972-10-09 | 1972-10-09 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE2350691A1 DE2350691A1 (de) | 1974-04-18 |
| DE2350691C2 true DE2350691C2 (de) | 1981-11-12 |
Family
ID=14279840
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE2350691A Expired DE2350691C2 (de) | 1972-10-09 | 1973-10-09 | Herstellung von Pankreas-Elastase |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US3904479A (en:Method) |
| JP (1) | JPS5021557B2 (en:Method) |
| AR (1) | AR200149A1 (en:Method) |
| DE (1) | DE2350691C2 (en:Method) |
| DK (1) | DK136610B (en:Method) |
| ES (1) | ES419496A1 (en:Method) |
| FR (1) | FR2202102B1 (en:Method) |
| GB (1) | GB1443059A (en:Method) |
| NL (1) | NL180334C (en:Method) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5261287A (en) * | 1975-11-08 | 1977-05-20 | Eisai Co Ltd | Preparation of pancreatic elastase |
| CN103667225B (zh) * | 2013-11-23 | 2016-08-17 | 青岛康原药业有限公司 | 一种制备弹性蛋白酶的方法 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1076776A (en) * | 1963-10-10 | 1967-07-19 | Rolland Lab A | Process for the extraction of elastase |
| US3691015A (en) * | 1970-03-18 | 1972-09-12 | Colgate Palmolive Co | Method for purifying enzymes |
-
1972
- 1972-10-09 JP JP47100656A patent/JPS5021557B2/ja not_active Expired
-
1973
- 1973-10-08 DK DK545673AA patent/DK136610B/da not_active IP Right Cessation
- 1973-10-09 NL NLAANVRAGE7313893,A patent/NL180334C/xx not_active IP Right Cessation
- 1973-10-09 GB GB4700273A patent/GB1443059A/en not_active Expired
- 1973-10-09 FR FR7336010A patent/FR2202102B1/fr not_active Expired
- 1973-10-09 US US404719A patent/US3904479A/en not_active Expired - Lifetime
- 1973-10-09 DE DE2350691A patent/DE2350691C2/de not_active Expired
- 1973-10-09 ES ES419496A patent/ES419496A1/es not_active Expired
- 1973-10-09 AR AR250442A patent/AR200149A1/es active
Also Published As
| Publication number | Publication date |
|---|---|
| US3904479A (en) | 1975-09-09 |
| NL7313893A (en:Method) | 1974-04-11 |
| ES419496A1 (es) | 1976-05-01 |
| DE2350691A1 (de) | 1974-04-18 |
| FR2202102B1 (en:Method) | 1977-05-27 |
| GB1443059A (en) | 1976-07-21 |
| FR2202102A1 (en:Method) | 1974-05-03 |
| NL180334C (nl) | 1987-02-02 |
| DK136610B (da) | 1977-10-31 |
| JPS5021557B2 (en:Method) | 1975-07-23 |
| JPS4966882A (en:Method) | 1974-06-28 |
| AR200149A1 (es) | 1974-10-24 |
| DK136610C (en:Method) | 1978-04-10 |
| NL180334B (nl) | 1986-09-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OD | Request for examination | ||
| D2 | Grant after examination |