DE2321784C2 - Verfahren zur Herstellung von vernetzten Gelose- oder Agarose-Gelen sowie deren Verwendung - Google Patents
Verfahren zur Herstellung von vernetzten Gelose- oder Agarose-Gelen sowie deren VerwendungInfo
- Publication number
- DE2321784C2 DE2321784C2 DE2321784A DE2321784A DE2321784C2 DE 2321784 C2 DE2321784 C2 DE 2321784C2 DE 2321784 A DE2321784 A DE 2321784A DE 2321784 A DE2321784 A DE 2321784A DE 2321784 C2 DE2321784 C2 DE 2321784C2
- Authority
- DE
- Germany
- Prior art keywords
- gel
- gelose
- agarose
- gels
- agarose gels
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000011543 agarose gel Substances 0.000 title claims description 8
- 238000000034 method Methods 0.000 title claims description 8
- 238000004519 manufacturing process Methods 0.000 title description 3
- 239000000499 gel Substances 0.000 claims description 36
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 6
- 239000003431 cross linking reagent Substances 0.000 claims description 2
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 18
- 229920000936 Agarose Polymers 0.000 description 14
- LZKGFGLOQNSMBS-UHFFFAOYSA-N 4,5,6-trichlorotriazine Chemical compound ClC1=NN=NC(Cl)=C1Cl LZKGFGLOQNSMBS-UHFFFAOYSA-N 0.000 description 10
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 239000000126 substance Substances 0.000 description 7
- 238000001962 electrophoresis Methods 0.000 description 6
- 239000011324 bead Substances 0.000 description 5
- 238000004587 chromatography analysis Methods 0.000 description 5
- 238000010828 elution Methods 0.000 description 5
- 230000001954 sterilising effect Effects 0.000 description 5
- 238000004659 sterilization and disinfection Methods 0.000 description 5
- 239000000203 mixture Substances 0.000 description 4
- 229910000033 sodium borohydride Inorganic materials 0.000 description 4
- 239000012279 sodium borohydride Substances 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 3
- 229920002307 Dextran Polymers 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 235000010419 agar Nutrition 0.000 description 3
- 239000008367 deionised water Substances 0.000 description 3
- 229910021641 deionized water Inorganic materials 0.000 description 3
- 150000004676 glycans Chemical class 0.000 description 3
- 239000008187 granular material Substances 0.000 description 3
- 238000006116 polymerization reaction Methods 0.000 description 3
- 230000004304 visual acuity Effects 0.000 description 3
- RYHBNJHYFVUHQT-UHFFFAOYSA-N 1,4-Dioxane Chemical compound C1COCCO1 RYHBNJHYFVUHQT-UHFFFAOYSA-N 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 150000001805 chlorine compounds Chemical class 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- MGNCLNQXLYJVJD-UHFFFAOYSA-N cyanuric chloride Chemical compound ClC1=NC(Cl)=NC(Cl)=N1 MGNCLNQXLYJVJD-UHFFFAOYSA-N 0.000 description 2
- 230000008030 elimination Effects 0.000 description 2
- 238000003379 elimination reaction Methods 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 230000000951 immunodiffusion Effects 0.000 description 2
- 238000000760 immunoelectrophoresis Methods 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 239000011049 pearl Substances 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- PMUNIMVZCACZBB-UHFFFAOYSA-N 2-hydroxyethylazanium;chloride Chemical compound Cl.NCCO PMUNIMVZCACZBB-UHFFFAOYSA-N 0.000 description 1
- BRLQWZUYTZBJKN-UHFFFAOYSA-N Epichlorohydrin Chemical compound ClCC1CO1 BRLQWZUYTZBJKN-UHFFFAOYSA-N 0.000 description 1
- 239000004593 Epoxy Substances 0.000 description 1
- 241000206672 Gelidium Species 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 238000013375 chromatographic separation Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- GKIPXFAANLTWBM-UHFFFAOYSA-N epibromohydrin Chemical compound BrCC1CO1 GKIPXFAANLTWBM-UHFFFAOYSA-N 0.000 description 1
- 125000003700 epoxy group Chemical group 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 238000002270 exclusion chromatography Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- 230000006855 networking Effects 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 229920000647 polyepoxide Polymers 0.000 description 1
- 150000004804 polysaccharides Polymers 0.000 description 1
- 239000012429 reaction media Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 229940038773 trisodium citrate Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0036—Galactans; Derivatives thereof
- C08B37/0039—Agar; Agarose, i.e. D-galactose, 3,6-anhydro-D-galactose, methylated, sulfated, e.g. from the red algae Gelidium and Gracilaria; Agaropectin; Derivatives thereof, e.g. Sepharose, i.e. crosslinked agarose
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Polymers & Plastics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Materials Engineering (AREA)
- General Engineering & Computer Science (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB2009172A GB1435508A (en) | 1972-05-01 | 1972-05-01 | Process for the preparation of crosslinked polysaccharide gels |
| JP20167574 | 1974-12-24 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE2321784A1 DE2321784A1 (de) | 1973-11-15 |
| DE2321784C2 true DE2321784C2 (de) | 1983-09-15 |
Family
ID=26254436
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE2321784A Expired DE2321784C2 (de) | 1972-05-01 | 1973-04-30 | Verfahren zur Herstellung von vernetzten Gelose- oder Agarose-Gelen sowie deren Verwendung |
Country Status (5)
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4851395A (en) * | 1976-07-07 | 1989-07-25 | Kureha Kagaku Kogyo Kabushiki Kausha | Nitrogen-containing polysaccharide |
| US4312727A (en) * | 1979-03-23 | 1982-01-26 | The Cleveland Clinic Foundation | Glyoxal agarose and zonal immobilization of proteins therewith |
| SE7908454L (sv) * | 1979-10-11 | 1981-04-12 | Pharmacia Fine Chemicals Ab | Medium for isoelektrisk fokusering |
| US4319975A (en) * | 1980-10-20 | 1982-03-16 | Fmc Corporation | Derivatized agarose and method of making and using same |
| US4321121A (en) * | 1981-01-19 | 1982-03-23 | Beckman Instruments, Inc. | Electrophoretic technique for separating lactate dehydrogenase isoenzymes and improved electrophoretic gel for use therein |
| US4319976A (en) * | 1981-01-19 | 1982-03-16 | Beckman Instruments, Inc. | Electrophoretic technique for separating serum proteins and improved electrophoretic gel for use therein |
| JPS6372983U (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * | 1986-10-31 | 1988-05-16 | ||
| ES2118219T3 (es) * | 1991-12-20 | 1998-09-16 | Allied Signal Inc | Materiales de baja densidad que tienen alta superficie especifica, y articulos formados a partir de ellos para uso en la recuperacion de metales. |
| US5371208A (en) * | 1992-12-30 | 1994-12-06 | Guest Elchrom Scientific Ltd. | Preparation of cross-linked linear polysaccharide polymers as gels for electrophoresis |
| GB0316742D0 (en) * | 2003-07-17 | 2003-08-20 | Fermentas Uab | Electrophoretic gels and their manufacture |
| GB2464664B (en) * | 2008-10-20 | 2013-06-05 | Bpb Ltd | Gypsum plasterboard comprising s-triazine-crosslinked starch |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2805220A (en) * | 1954-08-16 | 1957-09-03 | Anheuser Busch | High viscosity and insoluble starch products and method for producing same |
| US3021329A (en) * | 1958-11-28 | 1962-02-13 | Miles Lab | Dialdehyde starch-melamine derivatives |
| US3507851A (en) * | 1966-03-24 | 1970-04-21 | Victor Ghetie | Synthesis derivatives of agarose having application in electrophoresis on gel and in chromatography |
| US3455895A (en) * | 1966-10-10 | 1969-07-15 | Cons Paper Inc | Triazine derivatives of spent sulfite liquor |
| US3637685A (en) * | 1967-05-15 | 1972-01-25 | Monacelli Walter J | Triamino-s-triazines |
| DE1916107B2 (de) * | 1968-03-30 | 1974-02-28 | Institutul De Biochimie Al Academiei Rsr, Bukarest | Verfahren zur Herstellung von basische Ionenaustauschern auf Agarosebasis |
| CH564040A5 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * | 1970-06-25 | 1975-07-15 | Exploaterings Ab Tbf |
-
1972
- 1972-05-01 GB GB2009172A patent/GB1435508A/en not_active Expired
-
1973
- 1973-04-25 FR FR7314894A patent/FR2183009B1/fr not_active Expired
- 1973-04-30 US US05/355,481 patent/US3956272A/en not_active Expired - Lifetime
- 1973-04-30 DE DE2321784A patent/DE2321784C2/de not_active Expired
-
1974
- 1974-12-24 JP JP752016A patent/JPS5337417B2/ja not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| US3956272A (en) | 1976-05-11 |
| JPS5337417B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1978-10-09 |
| GB1435508A (en) | 1976-05-12 |
| FR2183009B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1977-02-04 |
| JPS5176444A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1976-07-02 |
| FR2183009A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1973-12-14 |
| DE2321784A1 (de) | 1973-11-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8181 | Inventor (new situation) |
Free format text: TIXIER, RENE, PARIS, FR |
|
| 8125 | Change of the main classification |
Ipc: C08B 37/12 |
|
| 8126 | Change of the secondary classification |
Ipc: B01D 15/08 |
|
| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |