DE2321050A1 - Verfahren und einrichtung zur ermittlung boesartiger zellen - Google Patents
Verfahren und einrichtung zur ermittlung boesartiger zellenInfo
- Publication number
- DE2321050A1 DE2321050A1 DE2321050A DE2321050A DE2321050A1 DE 2321050 A1 DE2321050 A1 DE 2321050A1 DE 2321050 A DE2321050 A DE 2321050A DE 2321050 A DE2321050 A DE 2321050A DE 2321050 A1 DE2321050 A1 DE 2321050A1
- Authority
- DE
- Germany
- Prior art keywords
- gallium
- cells
- solution
- cell
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims description 71
- 229910052733 gallium Inorganic materials 0.000 claims description 118
- GYHNNYVSQQEPJS-UHFFFAOYSA-N Gallium Chemical compound [Ga] GYHNNYVSQQEPJS-UHFFFAOYSA-N 0.000 claims description 114
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 43
- 238000005406 washing Methods 0.000 claims description 32
- 230000003211 malignant effect Effects 0.000 claims description 31
- 230000002285 radioactive effect Effects 0.000 claims description 24
- 230000005855 radiation Effects 0.000 claims description 23
- MCJGNVYPOGVAJF-UHFFFAOYSA-N quinolin-8-ol Chemical compound C1=CN=C2C(O)=CC=CC2=C1 MCJGNVYPOGVAJF-UHFFFAOYSA-N 0.000 claims description 16
- 238000005259 measurement Methods 0.000 claims description 14
- 238000010521 absorption reaction Methods 0.000 claims description 12
- 239000000463 material Substances 0.000 claims description 10
- 238000012360 testing method Methods 0.000 claims description 10
- 238000001556 precipitation Methods 0.000 claims description 8
- 238000000149 argon plasma sintering Methods 0.000 claims description 7
- 239000002244 precipitate Substances 0.000 claims description 7
- LGDFHDKSYGVKDC-UHFFFAOYSA-N 8-hydroxyquinoline-5-sulfonic acid Chemical compound C1=CN=C2C(O)=CC=C(S(O)(=O)=O)C2=C1 LGDFHDKSYGVKDC-UHFFFAOYSA-N 0.000 claims description 6
- -1 gallium citrates Chemical class 0.000 claims description 6
- 150000002259 gallium compounds Chemical class 0.000 claims description 6
- 238000001514 detection method Methods 0.000 claims description 5
- 150000002258 gallium Chemical class 0.000 claims description 5
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 238000010186 staining Methods 0.000 claims description 4
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- 230000003595 spectral effect Effects 0.000 claims 2
- 239000005725 8-Hydroxyquinoline Substances 0.000 claims 1
- HVXPHYSLKISNEG-UHFFFAOYSA-N 8-hydroxyquinoline-6-sulfonic acid Chemical compound C1=CN=C2C(O)=CC(S(O)(=O)=O)=CC2=C1 HVXPHYSLKISNEG-UHFFFAOYSA-N 0.000 claims 1
- 239000000834 fixative Substances 0.000 claims 1
- 229960003540 oxyquinoline Drugs 0.000 claims 1
- 239000012857 radioactive material Substances 0.000 claims 1
- 239000000941 radioactive substance Substances 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 172
- 239000000243 solution Substances 0.000 description 68
- 235000019441 ethanol Nutrition 0.000 description 30
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 15
- 239000000725 suspension Substances 0.000 description 15
- 230000008569 process Effects 0.000 description 13
- GYHNNYVSQQEPJS-OIOBTWANSA-N Gallium-67 Chemical compound [67Ga] GYHNNYVSQQEPJS-OIOBTWANSA-N 0.000 description 12
- 230000001413 cellular effect Effects 0.000 description 12
- 239000003795 chemical substances by application Substances 0.000 description 12
- 150000001875 compounds Chemical class 0.000 description 12
- 229940006110 gallium-67 Drugs 0.000 description 12
- 239000003153 chemical reaction reagent Substances 0.000 description 10
- 238000001914 filtration Methods 0.000 description 10
- 238000005119 centrifugation Methods 0.000 description 9
- 230000000694 effects Effects 0.000 description 8
- 206010028980 Neoplasm Diseases 0.000 description 7
- 238000004458 analytical method Methods 0.000 description 7
- 239000007864 aqueous solution Substances 0.000 description 7
- 239000006285 cell suspension Substances 0.000 description 7
- 229910052742 iron Inorganic materials 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- WHKZBVQIMVUGIH-UHFFFAOYSA-N 3-hydroxyquinoline-2-carboxylic acid Chemical compound C1=CC=C2C=C(O)C(C(=O)O)=NC2=C1 WHKZBVQIMVUGIH-UHFFFAOYSA-N 0.000 description 6
- 230000001476 alcoholic effect Effects 0.000 description 6
- 201000011510 cancer Diseases 0.000 description 6
- 239000000839 emulsion Substances 0.000 description 6
- 150000003839 salts Chemical class 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 5
- 239000000644 isotonic solution Substances 0.000 description 5
- 230000036210 malignancy Effects 0.000 description 5
- OKKJLVBELUTLKV-UHFFFAOYSA-N methanol Natural products OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 5
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 239000003855 balanced salt solution Substances 0.000 description 4
- 230000001086 cytosolic effect Effects 0.000 description 4
- 150000002500 ions Chemical class 0.000 description 4
- 230000007935 neutral effect Effects 0.000 description 4
- 210000004940 nucleus Anatomy 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- YEEGWNXDUZONAA-UHFFFAOYSA-K 5-hydroxy-2,8,9-trioxa-1-gallabicyclo[3.3.2]decane-3,7,10-trione Chemical compound [Ga+3].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YEEGWNXDUZONAA-UHFFFAOYSA-K 0.000 description 3
- UHBIKXOBLZWFKM-UHFFFAOYSA-N 8-hydroxy-2-quinolinecarboxylic acid Chemical class C1=CC=C(O)C2=NC(C(=O)O)=CC=C21 UHBIKXOBLZWFKM-UHFFFAOYSA-N 0.000 description 3
- 108010077544 Chromatin Proteins 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 3
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 3
- 230000002159 abnormal effect Effects 0.000 description 3
- 239000011575 calcium Substances 0.000 description 3
- 229910052791 calcium Inorganic materials 0.000 description 3
- 210000003483 chromatin Anatomy 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 238000007654 immersion Methods 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 239000011777 magnesium Substances 0.000 description 3
- 229910052749 magnesium Inorganic materials 0.000 description 3
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 3
- 230000001575 pathological effect Effects 0.000 description 3
- 239000012266 salt solution Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000011573 trace mineral Substances 0.000 description 3
- 235000013619 trace mineral Nutrition 0.000 description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- 201000009030 Carcinoma Diseases 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 2
- NINIDFKCEFEMDL-AKLPVKDBSA-N Sulfur-35 Chemical compound [35S] NINIDFKCEFEMDL-AKLPVKDBSA-N 0.000 description 2
- 229910052782 aluminium Inorganic materials 0.000 description 2
- 230000008033 biological extinction Effects 0.000 description 2
- 125000004432 carbon atom Chemical group C* 0.000 description 2
- 210000003679 cervix uteri Anatomy 0.000 description 2
- 238000012937 correction Methods 0.000 description 2
- 239000007822 coupling agent Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 210000002919 epithelial cell Anatomy 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- UPWPDUACHOATKO-UHFFFAOYSA-K gallium trichloride Chemical compound Cl[Ga](Cl)Cl UPWPDUACHOATKO-UHFFFAOYSA-K 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000009595 pap smear Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 2
- 239000001509 sodium citrate Substances 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 239000008096 xylene Substances 0.000 description 2
- 206010049522 Anisochromia Diseases 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000007900 aqueous suspension Substances 0.000 description 1
- 238000000376 autoradiography Methods 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000001680 brushing effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 230000004700 cellular uptake Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000013522 chelant Substances 0.000 description 1
- 239000012568 clinical material Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000002380 cytological effect Effects 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 238000005206 flow analysis Methods 0.000 description 1
- 239000003269 fluorescent indicator Substances 0.000 description 1
- CKHJYUSOUQDYEN-UHFFFAOYSA-N gallium(3+) Chemical compound [Ga+3] CKHJYUSOUQDYEN-UHFFFAOYSA-N 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000000214 mouth Anatomy 0.000 description 1
- 230000001613 neoplastic effect Effects 0.000 description 1
- 230000001473 noxious effect Effects 0.000 description 1
- 210000000633 nuclear envelope Anatomy 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 238000011120 smear test Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 210000004291 uterus Anatomy 0.000 description 1
- 210000001215 vagina Anatomy 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 229940089401 xylon Drugs 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/60—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances involving radioactive labelled substances
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Physiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US00247740A US3857033A (en) | 1972-04-26 | 1972-04-26 | Detecting malignant cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2321050A1 true DE2321050A1 (de) | 1973-11-08 |
Family
ID=22936175
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE2321050A Pending DE2321050A1 (de) | 1972-04-26 | 1973-04-26 | Verfahren und einrichtung zur ermittlung boesartiger zellen |
Country Status (4)
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL43224A (en) * | 1973-09-14 | 1979-07-25 | Yeda Res & Dev | Fluorescence polarization measurements |
| US4070576A (en) * | 1976-02-02 | 1978-01-24 | American Science & Engineering, Inc. | Detecting malignant cells |
| US4112301A (en) * | 1976-06-03 | 1978-09-05 | American Science And Engineering, Inc. | Moving particles suspended in a carrier fluid through a flow channel having an input end under gas pressure |
| US4044253A (en) * | 1976-06-04 | 1977-08-23 | The United States Of America As Represented By The Secretary Of The Air Force | Non-destructive inspection of composite and adhesively bonded structures |
| US6026174A (en) * | 1992-10-14 | 2000-02-15 | Accumed International, Inc. | System and method for automatically detecting malignant cells and cells having malignancy-associated changes |
| US6051393A (en) * | 1994-03-29 | 2000-04-18 | Jones; Sonja K. | Method of detecting malignant and pre-malignant conditions of the cervix, and test kits therefor |
| US20140242600A1 (en) * | 2011-06-08 | 2014-08-28 | The Board Of Trustees Of The Leland Stanford Junior University | Imaging the heterogeneous uptake of radiolabeled molecules in single living cells |
| US10175219B2 (en) | 2014-07-28 | 2019-01-08 | The Board Of Trustees Of The Leland Stanford Junior University | Method to sort cells on the basis of radionuclide uptake |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3497690A (en) * | 1967-09-21 | 1970-02-24 | Bausch & Lomb | Method and apparatus for classifying biological cells by measuring the size and fluorescent response thereof |
| US3673410A (en) * | 1969-06-25 | 1972-06-27 | John H Waite | Method of examination of cell samples using a radioactively tagged dye |
| US3678148A (en) * | 1969-06-26 | 1972-07-18 | Information Utilization Corp | Radioactive slide specimen analysis of and method of preparation |
| US3586859A (en) * | 1970-02-16 | 1971-06-22 | Irwin J Katz | Fluorescent cell viability counter |
-
1972
- 1972-04-26 US US00247740A patent/US3857033A/en not_active Expired - Lifetime
-
1973
- 1973-04-26 DE DE2321050A patent/DE2321050A1/de active Pending
- 1973-04-26 FR FR7315202A patent/FR2182154B1/fr not_active Expired
- 1973-04-26 GB GB1991173A patent/GB1432834A/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| FR2182154A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1973-12-07 |
| GB1432834A (en) | 1976-04-22 |
| US3857033A (en) | 1974-12-24 |
| FR2182154B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1977-08-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE69838560T2 (de) | Essigsäure als signalverbesserendes kontrastmittel in der fluoreszenzspektroscopie | |
| DE69223931T2 (de) | Reagenszusammensetzungen und ihre Verwendung bei der Identifizierung und Charakterisierung von Retikulozyten in Vollblut | |
| DE3751859T2 (de) | Durchflusszytometrie-Verfahren zur Klassifikation von Leukozyten und Reagenzien dafür | |
| DE2134910C2 (de) | Verfahren zur Blutanalyse | |
| DE3026185C2 (de) | Zusammensetzung, geeignet zur Untersuchung biologischer Gewebe oder Flüssigkeiten und Verfahren zu deren Anwendung | |
| DE3109252C2 (de) | Verfahren und Zusammensetzung zur Bestimmung einzelner Leukozyten durch metachromatische Farbstoffdifferentialabsorption | |
| DE69521006T2 (de) | Reagenz und Verfahren zur Analyse fester Bestandteile im Harn | |
| DE2451409C2 (de) | Verfahren zur Bestimmung spezieller weißer Blutkörperchen | |
| DE69213315T2 (de) | Reagenzien und Verfahren zur Zellanalyse im Harn | |
| DE69031682T2 (de) | Thionin Färbungstechnik | |
| DE4239016C2 (de) | Verfahren zum Bestimmen der Konzentration von freien Ionen innerhalb einer Zelle unter Verwendung eines Fluoreszenzindikatorfarbstoffs | |
| DE69120026T2 (de) | Verfahren zur Leukozytenklassifizierung unter Verwendung der Duchflusszytometrie | |
| DE2422016A1 (de) | Diagnose von krankheitszustaenden durch fluoreszenzmessungen unter verwendung von abtastlaserstrahlen | |
| DE2953524A1 (de) | Biologisches messverfahren | |
| DE2031447A1 (de) | Verfahren zur Prüfung von Zellproben | |
| DE3208629A1 (de) | Metachromatisches farbstoffabsorptionsverfahren zur differentiellen bestimmung der entwicklungsstufen von neutrophilen und granulozytischen zellen sowie anderen leukozyten | |
| DE1498824A1 (de) | Vorrichtung zur maschinellen Krebsdiagnose | |
| DE3304795C2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | ||
| DE2321050A1 (de) | Verfahren und einrichtung zur ermittlung boesartiger zellen | |
| EP3430565B1 (de) | Verfahren zur untersuchung verteilter objekte unter segmentierung eines übersichtbildes | |
| DE2649902C3 (de) | Autoradiografisches Verfahren zur Bestimmung physiologischer Veränderungen in Geweben | |
| DE3786175T2 (de) | Differentialbindung von potentialsempfindlichen materialien durch lymphocyten. | |
| DE69426965T2 (de) | Test zum Nachweis von Toxizität | |
| DE2742957A1 (de) | Mikroskop | |
| DE2341061C3 (de) | Verfahren zur laufenden Klassierung von krankheitsverdachtigen Zellproben für Diagnostikprüfungen |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OHJ | Non-payment of the annual fee |