DE2015504A1 - Aqs enzyme prepns stabilised by sugars - Google Patents
Aqs enzyme prepns stabilised by sugarsInfo
- Publication number
- DE2015504A1 DE2015504A1 DE19702015504 DE2015504A DE2015504A1 DE 2015504 A1 DE2015504 A1 DE 2015504A1 DE 19702015504 DE19702015504 DE 19702015504 DE 2015504 A DE2015504 A DE 2015504A DE 2015504 A1 DE2015504 A1 DE 2015504A1
- Authority
- DE
- Germany
- Prior art keywords
- protease
- saccharide
- enzyme
- solution
- activity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000004190 Enzymes Human genes 0.000 title description 41
- 108090000790 Enzymes Proteins 0.000 title description 41
- 235000000346 sugar Nutrition 0.000 title description 2
- 150000008163 sugars Chemical class 0.000 title 1
- 108091005804 Peptidases Proteins 0.000 claims abstract description 33
- 239000004365 Protease Substances 0.000 claims abstract description 33
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 26
- 150000001720 carbohydrates Chemical class 0.000 claims abstract description 20
- 229930006000 Sucrose Natural products 0.000 claims abstract description 13
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 12
- 239000000945 filler Substances 0.000 claims abstract description 11
- 239000008103 glucose Substances 0.000 claims abstract description 10
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 9
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims abstract description 8
- 239000008101 lactose Substances 0.000 claims abstract description 8
- 159000000007 calcium salts Chemical class 0.000 claims abstract description 7
- 239000000243 solution Substances 0.000 claims description 50
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 239000007864 aqueous solution Substances 0.000 claims description 14
- 238000002360 preparation method Methods 0.000 claims description 12
- 239000005720 sucrose Substances 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 11
- 239000012736 aqueous medium Substances 0.000 claims description 4
- 239000003599 detergent Substances 0.000 claims description 3
- 239000000843 powder Substances 0.000 abstract description 6
- 238000005406 washing Methods 0.000 abstract description 4
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 abstract description 2
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 abstract 1
- 235000013681 dietary sucrose Nutrition 0.000 abstract 1
- 229960004793 sucrose Drugs 0.000 abstract 1
- 229940088598 enzyme Drugs 0.000 description 39
- 239000003153 chemical reaction reagent Substances 0.000 description 31
- 230000000694 effects Effects 0.000 description 31
- 239000012153 distilled water Substances 0.000 description 10
- 235000002639 sodium chloride Nutrition 0.000 description 9
- 102000035195 Peptidases Human genes 0.000 description 7
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 7
- 230000007423 decrease Effects 0.000 description 7
- 150000003839 salts Chemical class 0.000 description 7
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 239000005018 casein Substances 0.000 description 6
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 6
- 235000021240 caseins Nutrition 0.000 description 6
- 238000010790 dilution Methods 0.000 description 6
- 239000012895 dilution Substances 0.000 description 6
- 239000000203 mixture Substances 0.000 description 5
- 229910052938 sodium sulfate Inorganic materials 0.000 description 5
- 235000011152 sodium sulphate Nutrition 0.000 description 5
- 239000000758 substrate Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 4
- 229910001424 calcium ion Inorganic materials 0.000 description 4
- 230000000977 initiatory effect Effects 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 150000002500 ions Chemical class 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 235000019832 sodium triphosphate Nutrition 0.000 description 3
- 230000000087 stabilizing effect Effects 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 229910052784 alkaline earth metal Inorganic materials 0.000 description 2
- -1 alkaline earth metal salts Chemical class 0.000 description 2
- 102000004139 alpha-Amylases Human genes 0.000 description 2
- 108090000637 alpha-Amylases Proteins 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 102000005158 Subtilisins Human genes 0.000 description 1
- 108010056079 Subtilisins Proteins 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 229940024171 alpha-amylase Drugs 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- VSGNNIFQASZAOI-UHFFFAOYSA-L calcium acetate Chemical compound [Ca+2].CC([O-])=O.CC([O-])=O VSGNNIFQASZAOI-UHFFFAOYSA-L 0.000 description 1
- 239000001639 calcium acetate Substances 0.000 description 1
- 235000011092 calcium acetate Nutrition 0.000 description 1
- 229960005147 calcium acetate Drugs 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 235000011148 calcium chloride Nutrition 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 229940079919 digestives enzyme preparation Drugs 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000003262 industrial enzyme Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 231100000989 no adverse effect Toxicity 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 239000000244 polyoxyethylene sorbitan monooleate Substances 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000012088 reference solution Substances 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 229910001415 sodium ion Inorganic materials 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000013112 stability test Methods 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 125000000185 sucrose group Chemical group 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38663—Stabilised liquid enzyme compositions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Enzymes And Modification Thereof (AREA)
- Detergent Compositions (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1828869 | 1969-04-09 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2015504A1 true DE2015504A1 (en) | 1970-10-29 |
Family
ID=10109887
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19702015504 Pending DE2015504A1 (en) | 1969-04-09 | 1970-04-01 | Aqs enzyme prepns stabilised by sugars |
Country Status (2)
| Country | Link |
|---|---|
| DE (1) | DE2015504A1 (en:Method) |
| NL (1) | NL7003678A (en:Method) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3225250A1 (de) * | 1981-07-06 | 1983-01-20 | Toyo Boseki K.K., Osaka | Stabile enzymzubereitung |
| DE3141498A1 (de) * | 1981-10-20 | 1983-04-28 | Bayer Ag, 5090 Leverkusen | Arzneimittel enthaltend kallikrein und verfahren zu seiner herstellung |
| WO2001096519A1 (en) * | 2000-06-15 | 2001-12-20 | Unilever N.V. | Liquid detergent composition |
| US9353335B2 (en) | 2013-11-11 | 2016-05-31 | Ecolab Usa Inc. | High alkaline warewash detergent with enhanced scale control and soil dispersion |
| US10179892B2 (en) | 2013-11-11 | 2019-01-15 | Ecolab Usa Inc. | Multiuse, enzymatic detergent and methods of stabilizing a use solution |
-
1970
- 1970-03-16 NL NL7003678A patent/NL7003678A/xx unknown
- 1970-04-01 DE DE19702015504 patent/DE2015504A1/de active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3225250A1 (de) * | 1981-07-06 | 1983-01-20 | Toyo Boseki K.K., Osaka | Stabile enzymzubereitung |
| DE3141498A1 (de) * | 1981-10-20 | 1983-04-28 | Bayer Ag, 5090 Leverkusen | Arzneimittel enthaltend kallikrein und verfahren zu seiner herstellung |
| WO2001096519A1 (en) * | 2000-06-15 | 2001-12-20 | Unilever N.V. | Liquid detergent composition |
| US9353335B2 (en) | 2013-11-11 | 2016-05-31 | Ecolab Usa Inc. | High alkaline warewash detergent with enhanced scale control and soil dispersion |
| US9683203B2 (en) | 2013-11-11 | 2017-06-20 | Ecolab Usa Inc. | High alkaline warewash detergent with enhanced scale control and soil dispersion |
| US10179892B2 (en) | 2013-11-11 | 2019-01-15 | Ecolab Usa Inc. | Multiuse, enzymatic detergent and methods of stabilizing a use solution |
| US10316272B2 (en) | 2013-11-11 | 2019-06-11 | Ecolab Usa Inc. | High alkaline warewash detergent with enhanced scale control and soil dispersion |
| US10597613B2 (en) | 2013-11-11 | 2020-03-24 | Ecolab Usa Inc. | Multiuse, enzymatic detergent and methods of stabilizing a use solution |
| US10745651B2 (en) | 2013-11-11 | 2020-08-18 | Ecolab Usa Inc. | High alkaline warewash detergent with enhanced scale control and soil dispersion |
| US11339354B2 (en) | 2013-11-11 | 2022-05-24 | Ecolab Usa Inc. | High alkaline warewash detergent with enhanced scale control and soil dispersion |
| US11920109B2 (en) | 2013-11-11 | 2024-03-05 | Ecolab Usa Inc. | High alkaline warewash detergent with enhanced scale control and soil dispersion |
| US12371640B2 (en) | 2013-11-11 | 2025-07-29 | Ecolab Usa Inc. | High alkaline warewash detergent with enhanced scale control and soil dispersion |
Also Published As
| Publication number | Publication date |
|---|---|
| NL7003678A (en:Method) | 1970-10-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE69409107T2 (de) | Reagenz zum gebrauch in biolumineszenz | |
| DE69003253T2 (de) | Enzymhaltige zubereitung und eine solche zubereitung enthaltendes detergens. | |
| DE68905117T2 (de) | Verfahren zur stabilisierung von blutgewinnungsfaktoren. | |
| DE60027406T2 (de) | Rückgewinnung eines proteins bei hohem ph-wert | |
| DE2315501A1 (de) | Verfahren zur bestimmung von cholesterin | |
| EP0201805B1 (de) | Stabilisiertes Sarcosinoxidase-Präparat | |
| DE2224131A1 (de) | Verfahren zur gewinnung von cholesterinoxydase | |
| EP0625581A1 (de) | Verfahren zum optischen Nachweis von Mikroorganismen, ihrer Identifizierung und der Empfindlichkeitstestung gegen Antibiotika unter Verwendung eines Redoxindikatorsystems | |
| DE60119965T2 (de) | Verfahren und medium zum nachweisen/identifizieren von mikroorganismen mit esterase- und/oder osidase- und/oder peptidase- und/oder sulfatase- und/oder phosphataseaktivität | |
| EP0058959A1 (de) | Verfahren zur Herstellung von trübgasarmen Eich- oder Kontrollseren | |
| DE2015504A1 (en) | Aqs enzyme prepns stabilised by sugars | |
| EP0065286B1 (de) | Verfahren zum Reinigen bzw. Anreichern von biologisch aktiven Proteinen und hierzu geeignetes Mittel | |
| DE1102973B (de) | Verfahren zur Herstellung von hoch gereinigten Kallikrein-Praeparaten | |
| DE69831508T2 (de) | Verfahren zur analyse intrazellulärer substanzen | |
| DE3636825A1 (de) | Verfahren zur herstellung von loesungsmittelstabiler (alpha)-amylase | |
| CH645670A5 (de) | Diagnostisches mittel. | |
| DE3854127T2 (de) | Stabiles alpha-amylase-reagenz. | |
| DE69219855T2 (de) | Verfahren zur Bestimmung der alpha-Amylase-Aktivität | |
| EP0173919B1 (de) | Verfahren zur mikrobiologischen Herstellung von Aldose-1-epimerase | |
| DE2239210C3 (de) | Verfahren zur Herstellung von a- Galactosidase, die eine starke a- Galactosidase-Aktivität und eine äußerst geringe Invertase-Aktivität aufweist, und deren Verwendung zur Zerlegung von Raffinose | |
| DE69030798T2 (de) | Stabilisierung des Glucose-Oxidase-Enzyms in flüssigem Reagenz | |
| AT336182B (de) | Verfahren zur herstellung eines prothrombinkomplexes | |
| DE3941054A1 (de) | Stabiles fluessiges enzymkonzentrat und verfahren zu seiner herstellung | |
| DE2626626B1 (de) | Verfahren zur Gewinnung von loeslicher Invertase aus Hefe | |
| DE2329921C2 (de) | Gewinnung hellfarbiger Proteasen |