DE19731292A1 - Nucleinsäuremolekül, Kit und Verwendung - Google Patents
Nucleinsäuremolekül, Kit und VerwendungInfo
- Publication number
- DE19731292A1 DE19731292A1 DE19731292A DE19731292A DE19731292A1 DE 19731292 A1 DE19731292 A1 DE 19731292A1 DE 19731292 A DE19731292 A DE 19731292A DE 19731292 A DE19731292 A DE 19731292A DE 19731292 A1 DE19731292 A1 DE 19731292A1
- Authority
- DE
- Germany
- Prior art keywords
- nucleic acid
- acid molecule
- bacteria
- sequence
- staphylococcus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 71
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 70
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 70
- 241000894006 Bacteria Species 0.000 claims abstract description 34
- 241000191940 Staphylococcus Species 0.000 claims abstract description 27
- 238000001514 detection method Methods 0.000 claims abstract description 25
- 241000191967 Staphylococcus aureus Species 0.000 claims abstract description 23
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 37
- 230000003321 amplification Effects 0.000 claims description 36
- 108020004414 DNA Proteins 0.000 claims description 26
- 239000002773 nucleotide Substances 0.000 claims description 25
- 125000003729 nucleotide group Chemical group 0.000 claims description 25
- 239000000523 sample Substances 0.000 claims description 24
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 21
- 238000006243 chemical reaction Methods 0.000 claims description 11
- 238000009396 hybridization Methods 0.000 claims description 11
- 108090000790 Enzymes Proteins 0.000 claims description 10
- 102000004190 Enzymes Human genes 0.000 claims description 10
- 108091029795 Intergenic region Proteins 0.000 claims description 9
- 230000014670 detection of bacterium Effects 0.000 claims description 7
- 239000002253 acid Substances 0.000 claims description 5
- 230000000295 complement effect Effects 0.000 claims description 5
- 239000000427 antigen Substances 0.000 claims description 3
- 108091007433 antigens Proteins 0.000 claims description 3
- 102000036639 antigens Human genes 0.000 claims description 3
- 238000007899 nucleic acid hybridization Methods 0.000 claims description 3
- 230000002285 radioactive effect Effects 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- 238000006911 enzymatic reaction Methods 0.000 claims description 2
- 238000003752 polymerase chain reaction Methods 0.000 claims description 2
- 239000007790 solid phase Substances 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 24
- 238000003149 assay kit Methods 0.000 abstract 1
- 108091034117 Oligonucleotide Proteins 0.000 description 22
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 20
- 244000005700 microbiome Species 0.000 description 16
- 239000000047 product Substances 0.000 description 16
- 238000012360 testing method Methods 0.000 description 9
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 8
- 241000894007 species Species 0.000 description 6
- 238000010561 standard procedure Methods 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 2
- 238000001712 DNA sequencing Methods 0.000 description 2
- RJQXTJLFIWVMTO-TYNCELHUSA-N Methicillin Chemical compound COC1=CC=CC(OC)=C1C(=O)N[C@@H]1C(=O)N2[C@@H](C(O)=O)C(C)(C)S[C@@H]21 RJQXTJLFIWVMTO-TYNCELHUSA-N 0.000 description 2
- 108010059724 Micrococcal Nuclease Proteins 0.000 description 2
- 239000004677 Nylon Substances 0.000 description 2
- 238000002105 Southern blotting Methods 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 229960003085 meticillin Drugs 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 210000003705 ribosome Anatomy 0.000 description 2
- 238000011895 specific detection Methods 0.000 description 2
- QRXMUCSWCMTJGU-UHFFFAOYSA-L (5-bromo-4-chloro-1h-indol-3-yl) phosphate Chemical compound C1=C(Br)C(Cl)=C2C(OP([O-])(=O)[O-])=CNC2=C1 QRXMUCSWCMTJGU-UHFFFAOYSA-L 0.000 description 1
- 108020004465 16S ribosomal RNA Proteins 0.000 description 1
- BMYCCWYAFNPAQC-UHFFFAOYSA-N 2-[dodecyl(methyl)azaniumyl]acetate Chemical compound CCCCCCCCCCCCN(C)CC(O)=O BMYCCWYAFNPAQC-UHFFFAOYSA-N 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 108010065152 Coagulase Proteins 0.000 description 1
- 241001478240 Coccus Species 0.000 description 1
- 230000004544 DNA amplification Effects 0.000 description 1
- 206010016952 Food poisoning Diseases 0.000 description 1
- 208000019331 Foodborne disease Diseases 0.000 description 1
- 108060004795 Methyltransferase Proteins 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 235000010678 Paulownia tomentosa Nutrition 0.000 description 1
- 240000002834 Paulownia tomentosa Species 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 108700005075 Regulator Genes Proteins 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 241001147687 Staphylococcus auricularis Species 0.000 description 1
- 241001147736 Staphylococcus capitis Species 0.000 description 1
- 241001147698 Staphylococcus cohnii Species 0.000 description 1
- 241000191963 Staphylococcus epidermidis Species 0.000 description 1
- 241000191984 Staphylococcus haemolyticus Species 0.000 description 1
- 241000192087 Staphylococcus hominis Species 0.000 description 1
- 241001464905 Staphylococcus saccharolyticus Species 0.000 description 1
- 241001147691 Staphylococcus saprophyticus Species 0.000 description 1
- 241000191978 Staphylococcus simulans Species 0.000 description 1
- 241000192086 Staphylococcus warneri Species 0.000 description 1
- 241000191973 Staphylococcus xylosus Species 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 108010059993 Vancomycin Proteins 0.000 description 1
- FJWGYAHXMCUOOM-QHOUIDNNSA-N [(2s,3r,4s,5r,6r)-2-[(2r,3r,4s,5r,6s)-4,5-dinitrooxy-2-(nitrooxymethyl)-6-[(2r,3r,4s,5r,6s)-4,5,6-trinitrooxy-2-(nitrooxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-3,5-dinitrooxy-6-(nitrooxymethyl)oxan-4-yl] nitrate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O)O[C@H]1[C@@H]([C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H](CO[N+]([O-])=O)O1)O[N+]([O-])=O)CO[N+](=O)[O-])[C@@H]1[C@@H](CO[N+]([O-])=O)O[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O FJWGYAHXMCUOOM-QHOUIDNNSA-N 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 230000004520 agglutination Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 210000001217 buttock Anatomy 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000147 enterotoxin Substances 0.000 description 1
- 231100000655 enterotoxin Toxicity 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000007834 ligase chain reaction Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000013048 microbiological method Methods 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 229940079938 nitrocellulose Drugs 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- -1 nucleoside triphosphates Chemical class 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 229920002477 rna polymer Polymers 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 229940037649 staphylococcus haemolyticus Drugs 0.000 description 1
- 229940037648 staphylococcus simulans Drugs 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- MYPYJXKWCTUITO-LYRMYLQWSA-N vancomycin Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=C2C=C3C=C1OC1=CC=C(C=C1Cl)[C@@H](O)[C@H](C(N[C@@H](CC(N)=O)C(=O)N[C@H]3C(=O)N[C@H]1C(=O)N[C@H](C(N[C@@H](C3=CC(O)=CC(O)=C3C=3C(O)=CC=C1C=3)C(O)=O)=O)[C@H](O)C1=CC=C(C(=C1)Cl)O2)=O)NC(=O)[C@@H](CC(C)C)NC)[C@H]1C[C@](C)(N)[C@H](O)[C@H](C)O1 MYPYJXKWCTUITO-LYRMYLQWSA-N 0.000 description 1
- MYPYJXKWCTUITO-UHFFFAOYSA-N vancomycin Natural products O1C(C(=C2)Cl)=CC=C2C(O)C(C(NC(C2=CC(O)=CC(O)=C2C=2C(O)=CC=C3C=2)C(O)=O)=O)NC(=O)C3NC(=O)C2NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(CC(C)C)NC)C(O)C(C=C3Cl)=CC=C3OC3=CC2=CC1=C3OC1OC(CO)C(O)C(O)C1OC1CC(C)(N)C(O)C(C)O1 MYPYJXKWCTUITO-UHFFFAOYSA-N 0.000 description 1
- 229960003165 vancomycin Drugs 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Saccharide Compounds (AREA)
Priority Applications (8)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19731292A DE19731292A1 (de) | 1997-07-21 | 1997-07-21 | Nucleinsäuremolekül, Kit und Verwendung |
| PCT/EP1998/004510 WO1999005159A2 (de) | 1997-07-21 | 1998-07-21 | Staphylococcus-spezifisches nucleinsäuremolekül, kit und verwendung nucleinsäuremolekül, nucleinsauremolekul, kit und verwendung |
| JP2000504153A JP2001510688A (ja) | 1997-07-21 | 1998-07-21 | 核酸分子、キットおよび使用 |
| EP98941375A EP0998483B1 (de) | 1997-07-21 | 1998-07-21 | Nucleinsäuremolekül, kit und verwendung |
| AT98941375T ATE364616T1 (de) | 1997-07-21 | 1998-07-21 | Nucleinsäuremolekül, kit und verwendung |
| DE59814032T DE59814032D1 (de) | 1997-07-21 | 1998-07-21 | Nucleinsäuremolekül, kit und verwendung |
| AU18470/99A AU1847099A (en) | 1997-07-21 | 1998-07-21 | Nucleic acid molecule, test kit and use |
| US09/463,209 US7002005B1 (en) | 1997-07-21 | 1998-07-21 | Analytical detection of Staphylococcus aureus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19731292A DE19731292A1 (de) | 1997-07-21 | 1997-07-21 | Nucleinsäuremolekül, Kit und Verwendung |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE19731292A1 true DE19731292A1 (de) | 1999-01-28 |
Family
ID=7836417
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19731292A Withdrawn DE19731292A1 (de) | 1997-07-21 | 1997-07-21 | Nucleinsäuremolekül, Kit und Verwendung |
| DE59814032T Expired - Lifetime DE59814032D1 (de) | 1997-07-21 | 1998-07-21 | Nucleinsäuremolekül, kit und verwendung |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE59814032T Expired - Lifetime DE59814032D1 (de) | 1997-07-21 | 1998-07-21 | Nucleinsäuremolekül, kit und verwendung |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US7002005B1 (https=) |
| EP (1) | EP0998483B1 (https=) |
| JP (1) | JP2001510688A (https=) |
| AT (1) | ATE364616T1 (https=) |
| AU (1) | AU1847099A (https=) |
| DE (2) | DE19731292A1 (https=) |
| WO (1) | WO1999005159A2 (https=) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19945916A1 (de) * | 1999-09-24 | 2001-04-05 | Biotecon Diagnostics Gmbh | Nukleinsäuremoleküle zum Nachweis von Bakterien und phylogenetischen Einheiten von Bakterien |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2684570A1 (en) | 2007-04-19 | 2008-10-30 | Molecular Detection Inc. | Methods, compositions and kits for detection and analysis of antibiotic-resistant bacteria |
| US8228855B2 (en) | 2008-03-24 | 2012-07-24 | Qualcomm Incorporated | Uplink power headroom definition for E-DCH in CELL—FACH |
| EP2287332B1 (en) * | 2009-08-07 | 2014-12-10 | Biotecon Diagnostics GmbH | Nucleic acids and methods for environmental monitoring |
| US20130158492A1 (en) * | 2011-12-20 | 2013-06-20 | Xuedong Song | Absorbent Article With Moisture Indicator |
| WO2013166460A1 (en) * | 2012-05-04 | 2013-11-07 | The Regents Of The University Of California | Antibiotic susceptibility testing using probes for preribosomal rna |
| MX2016000811A (es) * | 2013-07-23 | 2016-10-13 | Univ California | Amdinocilina para la rápida determinación de la susceptibilidad a antibióticos beta-lactámicos. |
| CN105695563A (zh) * | 2015-04-30 | 2016-06-22 | 汕头国际旅行卫生保健中心 | 一种用于大肠埃希菌和金黄色葡萄球菌23S-5S rRNA基因测序鉴定法的引物序列 |
| CN120099202B (zh) * | 2025-05-09 | 2025-08-05 | 江西省检验检测认证总院 | 用于筛查木糖葡萄球菌的特异性分子标记、引物组及应用 |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1991008305A1 (en) * | 1989-11-27 | 1991-06-13 | U-Gene Research B.V. | Dna fragments and dna probes and primers based thereon |
| WO1992005281A1 (fr) * | 1990-09-14 | 1992-04-02 | Chugai Seiyaku Kabushiki Kaisha | Procede de detection de staphylococcus aureus resistant au mesitylene-cephem |
| WO1993003186A1 (en) * | 1991-07-31 | 1993-02-18 | F.Hoffmann-La Roche Ag | Methods and reagents for detection of bacteria in cerebrospinal fluid |
| EP0669399A2 (en) * | 1994-02-28 | 1995-08-30 | Shimadzu Corporation | Oligonucleotides and method for detecting bacteria |
| WO1996024686A2 (fr) * | 1995-02-08 | 1996-08-15 | Bio Merieux | Detection de bacteries du genre listeria selon des techniques d'hybridation de sondes nucleiques |
| WO1996041878A1 (en) * | 1995-06-13 | 1996-12-27 | The Australian National University | Nucleic acid molecule and its uses in determining pathogenicity of staphylococcus |
| FR2743367A1 (fr) * | 1995-10-10 | 1997-07-11 | Vandenesch Francois | Sequences nucleotidiques hybridant specifiquement avec respectivement une sequence nucleotidique genomique de staphylococcus pasteuri ou de staphylococcus capitis |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5370992A (en) * | 1992-03-18 | 1994-12-06 | Amoco Corporation | Nucleic acid probes and methods for detecting Yersinia enterocolitica |
| CA2031498A1 (en) * | 1989-05-23 | 1990-11-24 | Raymond M. Nietupski | Nucleic acid probes for the detection of staphylococcus aureus |
| US5474796A (en) * | 1991-09-04 | 1995-12-12 | Protogene Laboratories, Inc. | Method and apparatus for conducting an array of chemical reactions on a support surface |
| US5292874A (en) * | 1991-09-04 | 1994-03-08 | Gen-Probe Incorporated | Nucleic acid probes to Staphylococcus aureus |
| IL103935A0 (en) * | 1991-12-04 | 1993-05-13 | Du Pont | Method for the identification of microorganisms by the utilization of directed and arbitrary dna amplification |
| US5994066A (en) * | 1995-09-11 | 1999-11-30 | Infectio Diagnostic, Inc. | Species-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories |
| US6737248B2 (en) * | 1996-01-05 | 2004-05-18 | Human Genome Sciences, Inc. | Staphylococcus aureus polynucleotides and sequences |
-
1997
- 1997-07-21 DE DE19731292A patent/DE19731292A1/de not_active Withdrawn
-
1998
- 1998-07-21 AU AU18470/99A patent/AU1847099A/en not_active Abandoned
- 1998-07-21 WO PCT/EP1998/004510 patent/WO1999005159A2/de not_active Ceased
- 1998-07-21 JP JP2000504153A patent/JP2001510688A/ja active Pending
- 1998-07-21 US US09/463,209 patent/US7002005B1/en not_active Expired - Fee Related
- 1998-07-21 AT AT98941375T patent/ATE364616T1/de not_active IP Right Cessation
- 1998-07-21 EP EP98941375A patent/EP0998483B1/de not_active Expired - Lifetime
- 1998-07-21 DE DE59814032T patent/DE59814032D1/de not_active Expired - Lifetime
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1991008305A1 (en) * | 1989-11-27 | 1991-06-13 | U-Gene Research B.V. | Dna fragments and dna probes and primers based thereon |
| WO1992005281A1 (fr) * | 1990-09-14 | 1992-04-02 | Chugai Seiyaku Kabushiki Kaisha | Procede de detection de staphylococcus aureus resistant au mesitylene-cephem |
| WO1993003186A1 (en) * | 1991-07-31 | 1993-02-18 | F.Hoffmann-La Roche Ag | Methods and reagents for detection of bacteria in cerebrospinal fluid |
| EP0669399A2 (en) * | 1994-02-28 | 1995-08-30 | Shimadzu Corporation | Oligonucleotides and method for detecting bacteria |
| WO1996024686A2 (fr) * | 1995-02-08 | 1996-08-15 | Bio Merieux | Detection de bacteries du genre listeria selon des techniques d'hybridation de sondes nucleiques |
| WO1996041878A1 (en) * | 1995-06-13 | 1996-12-27 | The Australian National University | Nucleic acid molecule and its uses in determining pathogenicity of staphylococcus |
| FR2743367A1 (fr) * | 1995-10-10 | 1997-07-11 | Vandenesch Francois | Sequences nucleotidiques hybridant specifiquement avec respectivement une sequence nucleotidique genomique de staphylococcus pasteuri ou de staphylococcus capitis |
Non-Patent Citations (9)
| Title |
|---|
| Medline 92350943 [Re. Microlbiol. 143 (1992) 37-46] * |
| Medline 92392923 [Biochimie 74 (1992) 585-588] Medline 96156105 [J. Clinical Microbiol. 33 (1995)3091-3095] * |
| Medline 95269967 [FEMS Microbiol. Lett. 128 (1995)119-125] * |
| Medline 96291654 [FEMS Microbiol. Lett. 140 (1996)111-119] * |
| Medline 96407006 [Nucleic Acids Res. 24 (1996) 3381-3391] * |
| Medline 97005372 [J. Appl. Bacteriol. 80 (1996) 244-251] * |
| Medline 97034110 [Clinical-Infect. Diseases 23 (1996) 475-480] * |
| Medline 97102183 [Zentralblatt f. Bakteriol. 285 (1996) 20-28] * |
| Medline AN 97237711 [Microbiology, 143 (1997) 823-834] * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19945916A1 (de) * | 1999-09-24 | 2001-04-05 | Biotecon Diagnostics Gmbh | Nukleinsäuremoleküle zum Nachweis von Bakterien und phylogenetischen Einheiten von Bakterien |
| WO2001023606A3 (de) * | 1999-09-24 | 2002-09-06 | Biotecon Diagnostics Gmbh | Nukleinsäuremoleküle zum nachweis von bakterien und phylogenetischen einheiten von bakterien |
| US7202027B1 (en) | 1999-09-24 | 2007-04-10 | Biotecon Diagnostics Gmbh | Nucleic acid molecules for detecting bacteria and phylogenetic units of bacteria |
| CN100473728C (zh) * | 1999-09-24 | 2009-04-01 | 生物技术检测股份有限公司 | 用于检测细菌群和细菌种系发育中单位的核酸分子 |
Also Published As
| Publication number | Publication date |
|---|---|
| ATE364616T1 (de) | 2007-07-15 |
| AU1847099A (en) | 1999-02-16 |
| WO1999005159A2 (de) | 1999-02-04 |
| EP0998483A2 (de) | 2000-05-10 |
| US7002005B1 (en) | 2006-02-21 |
| EP0998483B1 (de) | 2007-06-13 |
| WO1999005159A3 (de) | 1999-08-19 |
| JP2001510688A (ja) | 2001-08-07 |
| DE59814032D1 (de) | 2007-07-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE69225333T2 (de) | Verfahren für den Nachweis von Mikroorganismen unter verwendung von direkter undwillkürlicher DNA Amplifikation. | |
| DE69534516T2 (de) | Gleichzeitiger nachweis, identifizierung und differenzierung von eubakterien unter verwendung eines hybridisierungs-assays | |
| DE69527154T2 (de) | Spezifische und universelle amplifizierungs-primer zur raschen bestimmung und identifizierung ueblicher bakterieller pathogene und antibiotikaresistenzgene in klinischen proben zur routinediagnose in mikrobiologie-laboratorien | |
| Gütler | Typing of Clostridium difficile strains by PCR-amplification of variable length 16S-23S rDNA spacer regions | |
| Grtler et al. | Typing of Staphylococcus aureus strains by PCR-amplification of variable-length 16S-23S rDNA spacer regions: characterization of spacer sequences | |
| Walker et al. | Multiplex strand displacement amplification (SDA) and detection of DNA sequences from Mycobacterium tuberculosis and other mycobacteria | |
| DE69937447T2 (de) | Verfahren zur erkennung von nukleinsäuren | |
| DE19515891C2 (de) | Gattungs- und speziesspezifische Identifizierung von Legionellen | |
| DE19945916A1 (de) | Nukleinsäuremoleküle zum Nachweis von Bakterien und phylogenetischen Einheiten von Bakterien | |
| DE69528263T2 (de) | Verwendung eines spezifischen Markers zur Detektion von Salmonellen mit PCR | |
| DE19731292A1 (de) | Nucleinsäuremolekül, Kit und Verwendung | |
| King et al. | Capillary electrophoresis single-strand conformation polymorphism analysis for monitoring soil bacteria | |
| EP1522595A2 (de) | Oligonukleotide, Verfahren und System zur Detektion von antibiotikaresistenzvermittelnden Genen in Mikroorganismen mittels der Echtzeit-PCR | |
| EP2373813B1 (de) | Verfahren zum nachweis methicillin-resistenter staphylococcus aureus (mrsa)-stämme | |
| EP1232286B1 (de) | Test auf mikroorganismen zur diagnose von sepsis | |
| EP1012156B1 (de) | Nucleinsäure-sequenzen und verfahren zum nachweis von bakterien der gattung pseudomonas aeruginosa | |
| DE19801661A1 (de) | Schnell-Detektionsverfahren für Organismen durch Längenbestimmung ausgewählter Nukleinsäurebereiche | |
| DE10030376A1 (de) | Neues Verfahren zum Auffinden neuer Wirkstoffe | |
| EP0977894B1 (de) | Verfahren und kit zur identifizierung antibiotika-resistenter bakterienstämme | |
| EP2129798A2 (de) | Organismusspezifisches hybridisierbares nucleinsäuremolekül | |
| EP1561823A1 (de) | Verfahren zum Nachweis von Einzelnukleotid-polymorphismen (SNP) in Genen des Arzneimittelmetabolismus und Testkit zur Durchführung des Verfahrens | |
| WO1994017203A1 (en) | Amplified dna fingerprinting method for detecting genomic variation | |
| EP0991781B1 (de) | Nukleinsäuremolekülsatz für salmonella-nachweis, nukleinsäuren, kit und verwendung | |
| AT503401B1 (de) | Microarray zur erkennung von pathogenen | |
| DE10051174A1 (de) | Verfahren zum Nachweis von Staphylokokken und dazu geeignete Mittel |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OP8 | Request for examination as to paragraph 44 patent law | ||
| 8127 | New person/name/address of the applicant |
Owner name: BIOTECON DIAGNOSTICS GMBH, 10589 BERLIN, DE |
|
| 8130 | Withdrawal |