DE102011055247A1 - Multianalyt-Reportersystem - Google Patents

Multianalyt-Reportersystem Download PDF

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Publication number
DE102011055247A1
DE102011055247A1 DE102011055247A DE102011055247A DE102011055247A1 DE 102011055247 A1 DE102011055247 A1 DE 102011055247A1 DE 102011055247 A DE102011055247 A DE 102011055247A DE 102011055247 A DE102011055247 A DE 102011055247A DE 102011055247 A1 DE102011055247 A1 DE 102011055247A1
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region
mediator
molecule
detection
probe
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DE102011055247A
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German (de)
English (en)
Inventor
Günter Roth
Simon Wadle
Bernd Faltin
Felix VON STETTEN
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Albert Ludwigs Universitaet Freiburg
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Albert Ludwigs Universitaet Freiburg
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Priority to DE102011055247A priority Critical patent/DE102011055247A1/de
Priority to EP12798176.9A priority patent/EP2776585B8/de
Priority to PCT/EP2012/072402 priority patent/WO2013079307A1/de
Priority to US14/357,574 priority patent/US11293053B2/en
Priority to ES12798176T priority patent/ES2698903T3/es
Priority to JP2014540506A priority patent/JP6265905B2/ja
Priority to CA2854427A priority patent/CA2854427A1/en
Priority to CN201280055550.2A priority patent/CN103946397B/zh
Priority to IN4645DEN2014 priority patent/IN2014DN04645A/en
Publication of DE102011055247A1 publication Critical patent/DE102011055247A1/de
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
DE102011055247A 2011-11-10 2011-11-10 Multianalyt-Reportersystem Withdrawn DE102011055247A1 (de)

Priority Applications (9)

Application Number Priority Date Filing Date Title
DE102011055247A DE102011055247A1 (de) 2011-11-10 2011-11-10 Multianalyt-Reportersystem
EP12798176.9A EP2776585B8 (de) 2011-11-10 2012-11-12 Bifunktionale oligonukleotidsonde zur universellen echtzeit multianalyt detektion
PCT/EP2012/072402 WO2013079307A1 (de) 2011-11-10 2012-11-12 Bifunktionale oligonukleotidsonde zur universellen echtzeit multianalytdetektion
US14/357,574 US11293053B2 (en) 2011-11-10 2012-11-12 Bifunctional oligonucleotide probe for universal real time multianalyte detection
ES12798176T ES2698903T3 (es) 2011-11-10 2012-11-12 Oligonucleótido bifuncional para la detección de multianalitos universal en tiempo real
JP2014540506A JP6265905B2 (ja) 2011-11-10 2012-11-12 普遍的リアルタイム多分析物検出用の二官能性オリゴヌクレオチドプローブ
CA2854427A CA2854427A1 (en) 2011-11-10 2012-11-12 Bifunctional oligonucleotide probe for universal real-time multianalyte detection
CN201280055550.2A CN103946397B (zh) 2011-11-10 2012-11-12 用于通用实时多重分析检测的双功能寡核苷酸探针
IN4645DEN2014 IN2014DN04645A (https=) 2011-11-10 2012-11-12

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102011055247A DE102011055247A1 (de) 2011-11-10 2011-11-10 Multianalyt-Reportersystem

Publications (1)

Publication Number Publication Date
DE102011055247A1 true DE102011055247A1 (de) 2013-05-16

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Family Applications (1)

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DE102011055247A Withdrawn DE102011055247A1 (de) 2011-11-10 2011-11-10 Multianalyt-Reportersystem

Country Status (9)

Country Link
US (1) US11293053B2 (https=)
EP (1) EP2776585B8 (https=)
JP (1) JP6265905B2 (https=)
CN (1) CN103946397B (https=)
CA (1) CA2854427A1 (https=)
DE (1) DE102011055247A1 (https=)
ES (1) ES2698903T3 (https=)
IN (1) IN2014DN04645A (https=)
WO (1) WO2013079307A1 (https=)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102012100824A1 (de) 2012-02-01 2013-09-05 Albert-Ludwigs-Universität Freiburg Gemultiplexte Digital PCR
EP3034619A1 (de) * 2014-12-16 2016-06-22 microBIOMix GmbH Modifizierte Oligonukleotide und Verfahren zum Einschleusen von Primern oder Kontrollmolekülen oder Sonden in geschlossene PCR-Systeme
CN108368557A (zh) * 2015-12-01 2018-08-03 Sd生物传感器株式会社 包含用于核酸等温扩增的报告染料、猝灭剂的基于等温的双功能性寡聚核苷酸及利用其的核酸扩增和测定方法

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013138937A1 (en) * 2012-03-20 2013-09-26 UNIVERSITé LAVAL A nucleic acid detection method comprising target specific indexing probes (tsip) comprising a releasable segment to be detected via fluorescence when bound to a capture probe
US20140272959A1 (en) * 2013-03-14 2014-09-18 President And Fellows Of Harvard College Methods of Hybridizing Probes to Genomic DNA
EP3011056B1 (en) * 2013-06-19 2019-03-06 Luminex Corporation Real-time multiplexed hydrolysis probe assay
CN113528623A (zh) * 2015-02-18 2021-10-22 卓异生物公司 用于单分子检测的测定及其应用
CN107024583B (zh) * 2016-01-29 2019-03-08 中国科学院苏州纳米技术与纳米仿生研究所 硫黄素t适配体、其筛选方法及应用
CA3049833A1 (en) * 2016-12-23 2018-06-28 Albert-Ludwigs-Universitat Freiburg Two-piece mediator probe
CN108823287B (zh) 2017-04-28 2019-04-12 厦门大学 一种检测靶核酸序列的方法
GB201713251D0 (en) * 2017-08-18 2017-10-04 Lgc Genomics Ltd Methods and kits for detection of nucleic acid molecules
CN111100935B (zh) * 2018-10-26 2023-03-31 厦门大学 一种细菌耐药基因检测的方法
KR20230098259A (ko) * 2020-10-29 2023-07-03 비오까르띠 엔브이 멀티플렉스 핵산 검출을 위한 제네릭 카트리지 및 방법
US20230348957A1 (en) * 2021-12-07 2023-11-02 Luminex Corporation Methods and compositions for nucleic acid analysis
CN116356075A (zh) * 2021-12-27 2023-06-30 迈克生物股份有限公司 一种用于检测的引物探针、引物探针组及其应用
US20250340928A1 (en) 2022-04-22 2025-11-06 Albert-Ludwigs-Universität Freiburg Nucleic acid detection in a pcr by means of a target-sequence-unspecific modular reporter complex
US20250270624A1 (en) 2022-04-22 2025-08-28 Hahn-Schickard-Gesellschaft Für Angewandte Forschung E. V. Pcr multiplexing through target sequence-independent reporter molecules with distinguishable signal strengths
EP4265734A1 (de) * 2022-04-22 2023-10-25 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Nukleinsäurenachweis in einer pcr mittels eines zielsequenz-unspezifischem modularer reporterkomplexes
EP4265735A1 (de) 2022-04-22 2023-10-25 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Pcr-multiplexing durch zielsequenz-unabhängige-reportermoleküle mit unterscheid-baren signalstäken
EP4372100A1 (en) 2022-11-15 2024-05-22 Stilla Technologies Data analysis method for multiplex digital pcr using color-combination
CN121941778A (zh) * 2023-07-31 2026-04-28 北京卡尤迪生物科技股份有限公司 用于核酸检测的组合物和方法
CN117144067A (zh) * 2023-10-31 2023-12-01 中国医学科学院北京协和医院 用于多重化核酸检测的组合物和方法

Citations (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4751177A (en) * 1985-06-13 1988-06-14 Amgen Methods and kits for performing nucleic acid hybridization assays
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US5653939A (en) 1991-11-19 1997-08-05 Massachusetts Institute Of Technology Optical and electrical methods and apparatus for molecule detection
WO1998003678A1 (en) * 1996-07-24 1998-01-29 Biotec Laboratories Limited DETECTION OF PSYCHROTROPHIC $i(BACILLUS)
US6099803A (en) 1994-07-07 2000-08-08 Nanogen, Inc. Advanced active electronic devices for molecular biological analysis and diagnostics
WO2001006012A1 (en) * 1999-07-14 2001-01-25 Packard Bioscience Company Derivative nucleic acids and uses thereof
US6238869B1 (en) 1997-12-19 2001-05-29 High Throughput Genomics, Inc. High throughput assay system
US6300070B1 (en) 1999-06-04 2001-10-09 Mosaic Technologies, Inc. Solid phase methods for amplifying multiple nucleic acids
US20010034025A1 (en) 1999-01-15 2001-10-25 Ljl Biosystems, Inc. Methods and apparatus for detecting polynucleotide hybridization
US20020110826A1 (en) * 2000-07-14 2002-08-15 Nanibhushan Dattagupta Nucleic acid hairpin probes and uses thereof
US20040014078A1 (en) * 2002-02-06 2004-01-22 James Xia Compositions and methods for rolling circle amplification
US20040101835A1 (en) * 2000-10-24 2004-05-27 Willis Thomas D. Direct multiplex characterization of genomic dna
DE10316159A1 (de) 2003-04-09 2004-10-28 Klapproth, Holger, Dr. 5`-3`-Exonuklease Assay zur Bestimmung der Konzentration von Nukleinsäuren
US20070122804A1 (en) * 2002-10-29 2007-05-31 Guoliang Fu Combined exponential and linear amplification

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5520449B2 (ja) 2007-03-06 2014-06-11 株式会社ビー・エム・エル 核酸の検出方法
WO2009117327A2 (en) 2008-03-15 2009-09-24 Hologic, Inc. Compositions and methods for analysis of nucleic acid molecules during amplification reactions
MX352460B (es) 2011-01-11 2017-11-24 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.

Patent Citations (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4751177A (en) * 1985-06-13 1988-06-14 Amgen Methods and kits for performing nucleic acid hybridization assays
US5653939A (en) 1991-11-19 1997-08-05 Massachusetts Institute Of Technology Optical and electrical methods and apparatus for molecule detection
US6099803A (en) 1994-07-07 2000-08-08 Nanogen, Inc. Advanced active electronic devices for molecular biological analysis and diagnostics
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
WO1998003678A1 (en) * 1996-07-24 1998-01-29 Biotec Laboratories Limited DETECTION OF PSYCHROTROPHIC $i(BACILLUS)
US6238869B1 (en) 1997-12-19 2001-05-29 High Throughput Genomics, Inc. High throughput assay system
US20010034025A1 (en) 1999-01-15 2001-10-25 Ljl Biosystems, Inc. Methods and apparatus for detecting polynucleotide hybridization
US6300070B1 (en) 1999-06-04 2001-10-09 Mosaic Technologies, Inc. Solid phase methods for amplifying multiple nucleic acids
WO2001006012A1 (en) * 1999-07-14 2001-01-25 Packard Bioscience Company Derivative nucleic acids and uses thereof
US20020110826A1 (en) * 2000-07-14 2002-08-15 Nanibhushan Dattagupta Nucleic acid hairpin probes and uses thereof
US20040101835A1 (en) * 2000-10-24 2004-05-27 Willis Thomas D. Direct multiplex characterization of genomic dna
US20040014078A1 (en) * 2002-02-06 2004-01-22 James Xia Compositions and methods for rolling circle amplification
US20070122804A1 (en) * 2002-10-29 2007-05-31 Guoliang Fu Combined exponential and linear amplification
DE10316159A1 (de) 2003-04-09 2004-10-28 Klapproth, Holger, Dr. 5`-3`-Exonuklease Assay zur Bestimmung der Konzentration von Nukleinsäuren

Non-Patent Citations (16)

* Cited by examiner, † Cited by third party
Title
Andresen, D., von Nickisch-Rosenegk, M. and Bier, F.F., 2009, Helicase dependent OnChipamplification and its use in multiplex pathogen detection, Clin. Chim. Acta, 403, 244-248
Baeumner, A.J. et al, 2004, A universal nucleic acid sequence biosensor with nanomolar detection limits, Anal. Chem., 76, 888-894.
BRUNK C.F. u.a.: Analysis of specific bacteria from environmental samples using a quantitative polymerase chain reaction. Curr. Issues Mol. Biol. (2002) 4 (1) 13-18
BRUNK C.F. u.a.: Analysis of specific bacteria from environmental samples using a quantitative polymerase chain reaction. Curr. Issues Mol. Biol. (2002) 4 (1) 13-18 *
Chen, Y. et al, 2004, Surface amplification of invasive cleavage products, J. Amer. Chem. Soc., 126, 3016-3017.
HUANG Q. u.a.: Multiplex fluorescence melting curve analysis for mutation detection with dual-labeled, self-quenched probes. In: PLoS One. (April 2011) (4): e19206, 1-9 *
Liu, H.P. et al, 2006, TaqMan probe array for quantitative detection of DNA targets, Nucleic Acids Research, 34.
Liu, Q. et al, 2007, Microarray-in-a-tube for detection of multiple viruses. Clin Chem, 53, 188-194
Lockett, M.R. et al, 2007, Molecular beacon-style hybridization assay for quantitative analysis of surface invasive cleavage reactions, Anal. Chem., 79, 6031-6036.
Lu, M.C. et al, 2002, A surface invasive cleavage assay for highly parallel SNP analysis, Hum. Mutat., 19, 416-422.
Nie, B. et al, 2006, Quantitative detection of individual cleaved DNA molecules on surfaces using gold nanoparticles and scanning electron microscope imaging, Anal. Chem., 78, 1528-1534.
Pemov, A. et al, 2005, DNA analysis with multiplex microarray-enhanced PCR. Nucleic Acids Res., 33, e11.
SUN C. u.a.: Electrochemical DNA biosensor based on proximity-dependent DNA ligation assays with DNAzyme amplification of hairpin substrate signal. In: Biosensors and Bioelectronics (2010) 25, 2483-2489 *
SUN C. u.a.: Electrochemical DNA biosensor based on proximity-dependent DNA ligation assays with DNAzyme amplification of hairpin substrate signal. In: Biosensors and Bioelectronics (2010) 25, 2483–2489
TSOURKAS A. u.a.: Shedding night on health and disease using molecular beacons. Brief. Funct. Genomic. Proteomic. (2003) 1 (4) 372-384 *
VAN DOORN R. u.a.: Robust Detection and Identification of Multiple Oomycetes and Fungi in Environmental Samples by Using a Novel Cleavable Padlock Probe-Based Ligation Detection Assay. In: Appl. Environ. Microbiol. (2009) 75 (12) 4185-93 *

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102012100824A1 (de) 2012-02-01 2013-09-05 Albert-Ludwigs-Universität Freiburg Gemultiplexte Digital PCR
EP3034619A1 (de) * 2014-12-16 2016-06-22 microBIOMix GmbH Modifizierte Oligonukleotide und Verfahren zum Einschleusen von Primern oder Kontrollmolekülen oder Sonden in geschlossene PCR-Systeme
WO2016096185A1 (de) * 2014-12-16 2016-06-23 Microbiomix Gmbh Modifizierte oligonukleotide und verfahren zum einschleusen von primern oder kontrollmolekülen oder sonden in geschlossene pcr-systeme
CN108368557A (zh) * 2015-12-01 2018-08-03 Sd生物传感器株式会社 包含用于核酸等温扩增的报告染料、猝灭剂的基于等温的双功能性寡聚核苷酸及利用其的核酸扩增和测定方法
EP3385396A4 (en) * 2015-12-01 2019-04-10 SD Biosensor Inc. REAGENT FOR ISOTHERMIC NUCLEIC ACID AMPAYMENT, ISOTHERM-BASED DOUBLE-FUNCTIONAL OLIGONUCLEOTIDE WITH QUENCHER AND METHOD FOR NUCLEIC ACID AMPLIFICATION AND MEASUREMENT AND USE THEREOF
CN108368557B (zh) * 2015-12-01 2022-02-15 Sd生物传感器株式会社 基于等温的双功能性寡聚核苷酸及利用其的核酸扩增和测定方法
US11795514B2 (en) 2015-12-01 2023-10-24 Sd Biosensor, Inc. Isothermal based-dual functional oligonucleotide including reporter dye, and quencher for isothermal nucleic acid amplification and measurement methods using
EP4328327A3 (en) * 2015-12-01 2024-06-05 SD Biosensor Inc. Reporter dye for isothermal nucleic acid amplification, isothermal-based dual functional oligonucleotide containing quencher, and method for nucleic acid amplification and measurement using same

Also Published As

Publication number Publication date
US20140315747A1 (en) 2014-10-23
IN2014DN04645A (https=) 2015-05-08
ES2698903T3 (es) 2019-02-06
JP2014533107A (ja) 2014-12-11
EP2776585B1 (de) 2018-08-22
CN103946397B (zh) 2021-03-16
WO2013079307A1 (de) 2013-06-06
CA2854427A1 (en) 2013-06-06
EP2776585B8 (de) 2019-04-10
JP6265905B2 (ja) 2018-01-24
US11293053B2 (en) 2022-04-05
CN103946397A (zh) 2014-07-23
EP2776585A1 (de) 2014-09-17

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