DE102005009832A1 - Objektiv und Mikroskop - Google Patents
Objektiv und Mikroskop Download PDFInfo
- Publication number
- DE102005009832A1 DE102005009832A1 DE200510009832 DE102005009832A DE102005009832A1 DE 102005009832 A1 DE102005009832 A1 DE 102005009832A1 DE 200510009832 DE200510009832 DE 200510009832 DE 102005009832 A DE102005009832 A DE 102005009832A DE 102005009832 A1 DE102005009832 A1 DE 102005009832A1
- Authority
- DE
- Germany
- Prior art keywords
- lens
- light
- layers
- illumination
- detection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000005286 illumination Methods 0.000 claims abstract description 66
- 238000001514 detection method Methods 0.000 claims abstract description 43
- 210000001747 pupil Anatomy 0.000 claims abstract description 19
- 238000000204 total internal reflection microscopy Methods 0.000 claims abstract description 7
- 239000011521 glass Substances 0.000 claims description 6
- 239000011248 coating agent Substances 0.000 claims description 4
- 238000000576 coating method Methods 0.000 claims description 4
- 230000005284 excitation Effects 0.000 description 6
- 230000003287 optical effect Effects 0.000 description 6
- 238000000034 method Methods 0.000 description 3
- 239000013307 optical fiber Substances 0.000 description 3
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 239000006059 cover glass Substances 0.000 description 2
- 230000002093 peripheral effect Effects 0.000 description 2
- 239000012466 permeate Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000004621 scanning probe microscopy Methods 0.000 description 2
- 238000000492 total internal reflection fluorescence microscopy Methods 0.000 description 2
- 230000007704 transition Effects 0.000 description 2
- 230000002411 adverse Effects 0.000 description 1
- 230000001609 comparable effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 230000010287 polarization Effects 0.000 description 1
- 210000004127 vitreous body Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/02—Objectives
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
- G02B21/082—Condensers for incident illumination only
- G02B21/084—Condensers for incident illumination only having annular illumination around the objective
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Microscoopes, Condenser (AREA)
- Lenses (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE200510009832 DE102005009832A1 (de) | 2005-03-01 | 2005-03-01 | Objektiv und Mikroskop |
| DE200650001206 DE502006001206D1 (de) | 2005-03-01 | 2006-01-23 | Objektiv und Mikroskop |
| EP20060001362 EP1698929B1 (de) | 2005-03-01 | 2006-01-23 | Objektiv und Mikroskop |
| JP2006043796A JP5039307B2 (ja) | 2005-03-01 | 2006-02-21 | 対物レンズおよび顕微鏡 |
| US11/363,916 US7486441B2 (en) | 2005-03-01 | 2006-02-28 | Objective and microscope |
| CNA2006100198198A CN1828357A (zh) | 2005-03-01 | 2006-03-01 | 物镜和显微镜 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE200510009832 DE102005009832A1 (de) | 2005-03-01 | 2005-03-01 | Objektiv und Mikroskop |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE102005009832A1 true DE102005009832A1 (de) | 2006-09-07 |
Family
ID=36218595
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE200510009832 Withdrawn DE102005009832A1 (de) | 2005-03-01 | 2005-03-01 | Objektiv und Mikroskop |
| DE200650001206 Expired - Lifetime DE502006001206D1 (de) | 2005-03-01 | 2006-01-23 | Objektiv und Mikroskop |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE200650001206 Expired - Lifetime DE502006001206D1 (de) | 2005-03-01 | 2006-01-23 | Objektiv und Mikroskop |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP1698929B1 (https=) |
| JP (1) | JP5039307B2 (https=) |
| CN (1) | CN1828357A (https=) |
| DE (2) | DE102005009832A1 (https=) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102010034122A1 (de) | 2010-08-12 | 2012-04-19 | Carl Zeiss Microlmaging Gmbh | Mikroskop, insbesondere für die TIRF-Mikroskopie |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7855844B2 (en) * | 2007-05-17 | 2010-12-21 | Mitutoyo Corporation | Objective lens and optical measuring device |
| JP5834638B2 (ja) * | 2011-09-02 | 2015-12-24 | 株式会社ニコン | 対物レンズユニット及びこの対物レンズユニットを有する走査型顕微鏡 |
| CN102818796B (zh) * | 2012-07-23 | 2016-01-27 | 苏州生物医学工程技术研究所 | 生物荧光显微检测仪器 |
| CN102818794B (zh) * | 2012-07-23 | 2016-01-27 | 苏州生物医学工程技术研究所 | 生物荧光显微检测仪器 |
| CN102818795B (zh) * | 2012-07-23 | 2015-08-26 | 苏州生物医学工程技术研究所 | 生物荧光显微检测仪器 |
| DE102017110638B3 (de) * | 2017-05-16 | 2018-09-27 | Leica Microsystems Cms Gmbh | Mikroskop und Mikroskopbeleuchtungsverfahren |
| DE102017214189A1 (de) * | 2017-08-15 | 2019-02-21 | Carl Zeiss Microscopy Gmbh | Verfahren zum Betrieb einer Mikroskopieranordnung und Mikroskopieranordnung mit einem ersten Mikroskop und mindestens einem weiteren Mikroskop |
| CN109100352B (zh) * | 2018-08-30 | 2024-07-23 | 天津港东科技股份有限公司 | 显微镜多功能液体测试装置 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4671463B2 (ja) | 2000-03-24 | 2011-04-20 | オリンパス株式会社 | 照明光学系及び照明光学系を備えた顕微鏡 |
| US6597499B2 (en) | 2001-01-25 | 2003-07-22 | Olympus Optical Co., Ltd. | Total internal reflection fluorescence microscope having a conventional white-light source |
| DE10108796A1 (de) | 2001-02-21 | 2002-09-05 | Zeiss Carl Jena Gmbh | Hochaperturiges Objektiv |
| DE10143481A1 (de) | 2001-09-05 | 2003-03-20 | Europ Lab Molekularbiolog | Mikroskop |
| JP2003098439A (ja) * | 2001-09-25 | 2003-04-03 | Olympus Optical Co Ltd | 観察切り替え可能な顕微鏡 |
| DE10217098B4 (de) | 2002-04-17 | 2004-04-15 | Carl Zeiss Jena Gmbh | Auflicht-Beleuchtungsanordnung für ein Mikroskop |
| DE10229935B4 (de) | 2002-07-04 | 2018-02-08 | Carl Zeiss Microscopy Gmbh | Mikroskopschieber zur Einkopplung von Licht in ein Mikroskop |
| DE10309269B4 (de) * | 2003-03-03 | 2005-06-02 | Till Photonics Gmbh | Vorrichtung für Totale Interne Reflexions-Mikroskopie |
| DE102004015587A1 (de) * | 2003-04-04 | 2004-11-11 | Olympus Corporation | Fluoreszenzmikroskop mit totaler interner Reflexion |
| DE10344410A1 (de) * | 2003-09-25 | 2005-04-28 | Leica Microsystems | Rastermikroskop mit evaneszenter Beleuchtung |
-
2005
- 2005-03-01 DE DE200510009832 patent/DE102005009832A1/de not_active Withdrawn
-
2006
- 2006-01-23 EP EP20060001362 patent/EP1698929B1/de not_active Expired - Lifetime
- 2006-01-23 DE DE200650001206 patent/DE502006001206D1/de not_active Expired - Lifetime
- 2006-02-21 JP JP2006043796A patent/JP5039307B2/ja not_active Expired - Fee Related
- 2006-03-01 CN CNA2006100198198A patent/CN1828357A/zh active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102010034122A1 (de) | 2010-08-12 | 2012-04-19 | Carl Zeiss Microlmaging Gmbh | Mikroskop, insbesondere für die TIRF-Mikroskopie |
| DE102010034122B4 (de) * | 2010-08-12 | 2020-03-26 | Carl Zeiss Microscopy Gmbh | Mikroskop und Objektiv, insbesondere für die TIRF-Mikroskopie |
Also Published As
| Publication number | Publication date |
|---|---|
| JP5039307B2 (ja) | 2012-10-03 |
| CN1828357A (zh) | 2006-09-06 |
| EP1698929A1 (de) | 2006-09-06 |
| EP1698929B1 (de) | 2008-07-30 |
| DE502006001206D1 (de) | 2008-09-11 |
| JP2006243723A (ja) | 2006-09-14 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8139 | Disposal/non-payment of the annual fee |