DE10061655A1 - In situ-Hybridisierungs-Anordnung zum spezifischen Nachweis von Mikroorganismen - Google Patents
In situ-Hybridisierungs-Anordnung zum spezifischen Nachweis von MikroorganismenInfo
- Publication number
- DE10061655A1 DE10061655A1 DE10061655A DE10061655A DE10061655A1 DE 10061655 A1 DE10061655 A1 DE 10061655A1 DE 10061655 A DE10061655 A DE 10061655A DE 10061655 A DE10061655 A DE 10061655A DE 10061655 A1 DE10061655 A1 DE 10061655A1
- Authority
- DE
- Germany
- Prior art keywords
- slide
- sample
- nucleic acid
- hybridization
- arrangement according
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 244000005700 microbiome Species 0.000 title claims abstract description 67
- 238000007901 in situ hybridization Methods 0.000 title claims abstract description 48
- 238000011895 specific detection Methods 0.000 title claims description 16
- 238000000034 method Methods 0.000 claims abstract description 91
- 238000009396 hybridization Methods 0.000 claims abstract description 89
- 239000000523 sample Substances 0.000 claims description 90
- 108020004711 Nucleic Acid Probes Proteins 0.000 claims description 60
- 239000002853 nucleic acid probe Substances 0.000 claims description 60
- 241000894006 Bacteria Species 0.000 claims description 38
- 238000005406 washing Methods 0.000 claims description 30
- 239000003550 marker Substances 0.000 claims description 17
- 239000010802 sludge Substances 0.000 claims description 14
- 150000007523 nucleic acids Chemical class 0.000 claims description 13
- 238000011534 incubation Methods 0.000 claims description 11
- 239000000203 mixture Substances 0.000 claims description 11
- 108020004707 nucleic acids Proteins 0.000 claims description 11
- 102000039446 nucleic acids Human genes 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 230000000295 complement effect Effects 0.000 claims description 9
- 238000001035 drying Methods 0.000 claims description 9
- 239000013642 negative control Substances 0.000 claims description 8
- 239000004033 plastic Substances 0.000 claims description 8
- 229920003023 plastic Polymers 0.000 claims description 8
- 239000013641 positive control Substances 0.000 claims description 8
- 239000002351 wastewater Substances 0.000 claims description 8
- 230000008569 process Effects 0.000 claims description 7
- 235000013305 food Nutrition 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 230000002759 chromosomal effect Effects 0.000 claims description 5
- 108020004999 messenger RNA Proteins 0.000 claims description 5
- -1 polyethylene Polymers 0.000 claims description 5
- 230000002285 radioactive effect Effects 0.000 claims description 5
- 230000007613 environmental effect Effects 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 235000013336 milk Nutrition 0.000 claims description 4
- 239000008267 milk Substances 0.000 claims description 4
- 210000004080 milk Anatomy 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 4
- 230000000087 stabilizing effect Effects 0.000 claims description 4
- 241000233866 Fungi Species 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- 235000015173 baked goods and baking mixes Nutrition 0.000 claims description 3
- 239000002537 cosmetic Substances 0.000 claims description 3
- 235000020188 drinking water Nutrition 0.000 claims description 3
- 239000003651 drinking water Substances 0.000 claims description 3
- 230000028327 secretion Effects 0.000 claims description 3
- 238000004065 wastewater treatment Methods 0.000 claims description 3
- 239000004698 Polyethylene Substances 0.000 claims description 2
- 239000004743 Polypropylene Substances 0.000 claims description 2
- 239000003570 air Substances 0.000 claims description 2
- 235000013365 dairy product Nutrition 0.000 claims description 2
- 230000003301 hydrolyzing effect Effects 0.000 claims description 2
- 229920000573 polyethylene Polymers 0.000 claims description 2
- 229920001155 polypropylene Polymers 0.000 claims description 2
- 239000002689 soil Substances 0.000 claims description 2
- 239000000834 fixative Substances 0.000 claims 1
- 235000013372 meat Nutrition 0.000 claims 1
- 239000000243 solution Substances 0.000 description 89
- 210000004027 cell Anatomy 0.000 description 39
- 230000001580 bacterial effect Effects 0.000 description 29
- 238000001514 detection method Methods 0.000 description 11
- 230000000813 microbial effect Effects 0.000 description 10
- 108090000623 proteins and genes Proteins 0.000 description 10
- 239000002773 nucleotide Substances 0.000 description 9
- 125000003729 nucleotide group Chemical group 0.000 description 9
- AZQWKYJCGOJGHM-UHFFFAOYSA-N 1,4-benzoquinone Chemical compound O=C1C=CC(=O)C=C1 AZQWKYJCGOJGHM-UHFFFAOYSA-N 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 8
- 241000894007 species Species 0.000 description 8
- 108020004414 DNA Proteins 0.000 description 6
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 6
- 108091034117 Oligonucleotide Proteins 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 6
- 210000003705 ribosome Anatomy 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical class CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
- 230000008901 benefit Effects 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 230000001419 dependent effect Effects 0.000 description 5
- 238000011065 in-situ storage Methods 0.000 description 5
- 238000011282 treatment Methods 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 239000007983 Tris buffer Substances 0.000 description 4
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 4
- 238000004364 calculation method Methods 0.000 description 4
- 230000014670 detection of bacterium Effects 0.000 description 4
- 239000003599 detergent Substances 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- 238000011156 evaluation Methods 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- 239000010865 sewage Substances 0.000 description 4
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 4
- 238000012800 visualization Methods 0.000 description 4
- 108020004465 16S ribosomal RNA Proteins 0.000 description 3
- BZTDTCNHAFUJOG-UHFFFAOYSA-N 6-carboxyfluorescein Chemical compound C12=CC=C(O)C=C2OC2=CC(O)=CC=C2C11OC(=O)C2=CC=C(C(=O)O)C=C21 BZTDTCNHAFUJOG-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 230000018044 dehydration Effects 0.000 description 3
- 238000006297 dehydration reaction Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000035515 penetration Effects 0.000 description 3
- NMHMNPHRMNGLLB-UHFFFAOYSA-N phloretic acid Chemical compound OC(=O)CCC1=CC=C(O)C=C1 NMHMNPHRMNGLLB-UHFFFAOYSA-N 0.000 description 3
- 238000011002 quantification Methods 0.000 description 3
- 229940083575 sodium dodecyl sulfate Drugs 0.000 description 3
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- 239000011534 wash buffer Substances 0.000 description 3
- GZCWLCBFPRFLKL-UHFFFAOYSA-N 1-prop-2-ynoxypropan-2-ol Chemical compound CC(O)COCC#C GZCWLCBFPRFLKL-UHFFFAOYSA-N 0.000 description 2
- YCCILVSKPBXVIP-UHFFFAOYSA-N 2-(4-hydroxyphenyl)ethanol Chemical compound OCCC1=CC=C(O)C=C1 YCCILVSKPBXVIP-UHFFFAOYSA-N 0.000 description 2
- JRBJSXQPQWSCCF-UHFFFAOYSA-N 3,3'-Dimethoxybenzidine Chemical compound C1=C(N)C(OC)=CC(C=2C=C(OC)C(N)=CC=2)=C1 JRBJSXQPQWSCCF-UHFFFAOYSA-N 0.000 description 2
- YRNWIFYIFSBPAU-UHFFFAOYSA-N 4-[4-(dimethylamino)phenyl]-n,n-dimethylaniline Chemical compound C1=CC(N(C)C)=CC=C1C1=CC=C(N(C)C)C=C1 YRNWIFYIFSBPAU-UHFFFAOYSA-N 0.000 description 2
- 102000013563 Acid Phosphatase Human genes 0.000 description 2
- 108010051457 Acid Phosphatase Proteins 0.000 description 2
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 2
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 108010015776 Glucose oxidase Proteins 0.000 description 2
- 239000004366 Glucose oxidase Substances 0.000 description 2
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 102000003992 Peroxidases Human genes 0.000 description 2
- 238000007605 air drying Methods 0.000 description 2
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 description 2
- 102000005936 beta-Galactosidase Human genes 0.000 description 2
- 108010005774 beta-Galactosidase Proteins 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 2
- 229940116332 glucose oxidase Drugs 0.000 description 2
- 235000019420 glucose oxidase Nutrition 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 235000013622 meat product Nutrition 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000003068 molecular probe Substances 0.000 description 2
- 239000002751 oligonucleotide probe Substances 0.000 description 2
- 108040007629 peroxidase activity proteins Proteins 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 230000006641 stabilisation Effects 0.000 description 2
- 238000011105 stabilization Methods 0.000 description 2
- JGVWCANSWKRBCS-UHFFFAOYSA-N tetramethylrhodamine thiocyanate Chemical compound [Cl-].C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=C(SC#N)C=C1C(O)=O JGVWCANSWKRBCS-UHFFFAOYSA-N 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 230000014616 translation Effects 0.000 description 2
- BCHIXGBGRHLSBE-UHFFFAOYSA-N (4-methyl-2-oxochromen-7-yl) dihydrogen phosphate Chemical compound C1=C(OP(O)(O)=O)C=CC2=C1OC(=O)C=C2C BCHIXGBGRHLSBE-UHFFFAOYSA-N 0.000 description 1
- GEYOCULIXLDCMW-UHFFFAOYSA-N 1,2-phenylenediamine Chemical compound NC1=CC=CC=C1N GEYOCULIXLDCMW-UHFFFAOYSA-N 0.000 description 1
- KUWPCJHYPSUOFW-YBXAARCKSA-N 2-nitrophenyl beta-D-galactoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CC=CC=C1[N+]([O-])=O KUWPCJHYPSUOFW-YBXAARCKSA-N 0.000 description 1
- KDXNYSZNOWTPLE-UHFFFAOYSA-N 3'-hydroxy-6'-methoxyspiro[2-benzofuran-3,9'-xanthene]-1-one Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(OC)=CC=C21 KDXNYSZNOWTPLE-UHFFFAOYSA-N 0.000 description 1
- AZKSAVLVSZKNRD-UHFFFAOYSA-M 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide Chemical compound [Br-].S1C(C)=C(C)N=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=CC=C1 AZKSAVLVSZKNRD-UHFFFAOYSA-M 0.000 description 1
- YUDPTGPSBJVHCN-DZQJYWQESA-N 4-methylumbelliferyl beta-D-galactoside Chemical compound C1=CC=2C(C)=CC(=O)OC=2C=C1O[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O YUDPTGPSBJVHCN-DZQJYWQESA-N 0.000 description 1
- 241000589291 Acinetobacter Species 0.000 description 1
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- MGIODCZGPVDROX-UHFFFAOYSA-N Cy5-bifunctional dye Chemical compound O=C1CCC(=O)N1OC(=O)CCCCCN1C2=CC=C(S(O)(=O)=O)C=C2C(C)(C)C1=CC=CC=CC(C(C1=CC(=CC=C11)S([O-])(=O)=O)(C)C)=[N+]1CCCCCC(=O)ON1C(=O)CCC1=O MGIODCZGPVDROX-UHFFFAOYSA-N 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 108020003215 DNA Probes Proteins 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 108091061960 Naked DNA Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241000282320 Panthera leo Species 0.000 description 1
- 229930040373 Paraformaldehyde Natural products 0.000 description 1
- 241000192142 Proteobacteria Species 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 241000190807 Thiothrix Species 0.000 description 1
- DZGWFCGJZKJUFP-UHFFFAOYSA-N Tyramine Natural products NCCC1=CC=C(O)C=C1 DZGWFCGJZKJUFP-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000001174 ascending effect Effects 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 235000014121 butter Nutrition 0.000 description 1
- 235000015155 buttermilk Nutrition 0.000 description 1
- 230000006727 cell loss Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 235000013351 cheese Nutrition 0.000 description 1
- PRQROPMIIGLWRP-BZSNNMDCSA-N chemotactic peptide Chemical compound CSCC[C@H](NC=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 PRQROPMIIGLWRP-BZSNNMDCSA-N 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000001687 destabilization Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 description 1
- SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000001177 diphosphate Substances 0.000 description 1
- VIYFPAMJCJLZKD-UHFFFAOYSA-L disodium;(4-nitrophenyl) phosphate Chemical compound [Na+].[Na+].[O-][N+](=O)C1=CC=C(OP([O-])([O-])=O)C=C1 VIYFPAMJCJLZKD-UHFFFAOYSA-L 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000010981 drying operation Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
- 239000008098 formaldehyde solution Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- CATMPQFFVNKDEY-AAEUAGOBSA-N gamma-Glu-Trp Chemical compound C1=CC=C2C(C[C@H](NC(=O)CC[C@H](N)C(O)=O)C(O)=O)=CNC2=C1 CATMPQFFVNKDEY-AAEUAGOBSA-N 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 210000004779 membrane envelope Anatomy 0.000 description 1
- KBOPZPXVLCULAV-UHFFFAOYSA-N mesalamine Chemical compound NC1=CC=C(O)C(C(O)=O)=C1 KBOPZPXVLCULAV-UHFFFAOYSA-N 0.000 description 1
- 229960004963 mesalazine Drugs 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 238000012543 microbiological analysis Methods 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000006225 natural substrate Substances 0.000 description 1
- 210000001331 nose Anatomy 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
- 238000013081 phylogenetic analysis Methods 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 229920002477 rna polymer Polymers 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 229960003732 tyramine Drugs 0.000 description 1
- 210000003708 urethra Anatomy 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 210000001215 vagina Anatomy 0.000 description 1
- 235000013618 yogurt Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10061655A DE10061655A1 (de) | 2000-12-11 | 2000-12-11 | In situ-Hybridisierungs-Anordnung zum spezifischen Nachweis von Mikroorganismen |
| CA002430894A CA2430894A1 (en) | 2000-12-11 | 2001-12-11 | In situ hybridization system for specifically detecting microorganisms |
| DE50106653T DE50106653D1 (de) | 2000-12-11 | 2001-12-11 | In situ-hybridisierungs-anordnung zum spezifischen nachweis von mikroorganismen |
| AT01991823T ATE298786T1 (de) | 2000-12-11 | 2001-12-11 | In situ-hybridisierungs-anordnung zum spezifischen nachweis von mikroorganismen |
| JP2002550112A JP2004523223A (ja) | 2000-12-11 | 2001-12-11 | 微生物を特異的に検出するためのｉｎｓｉｔｕハイブリダイゼーション装置 |
| AU2002231689A AU2002231689A1 (en) | 2000-12-11 | 2001-12-11 | In situ hybridization system for specifically detecting microorganisms |
| EP01991823A EP1341897B1 (de) | 2000-12-11 | 2001-12-11 | In situ-hybridisierungs-anordnung zum spezifischen nachweis von mikroorganismen |
| PCT/EP2001/014543 WO2002048398A2 (de) | 2000-12-11 | 2001-12-11 | In situ-hybridisierungs-anordnung zum spezifischen nachweis von mikroorganismen |
| US10/458,775 US20040038270A1 (en) | 2000-12-11 | 2003-06-10 | In situ hybridization arrangement for the specific detection of microorganisms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10061655A DE10061655A1 (de) | 2000-12-11 | 2000-12-11 | In situ-Hybridisierungs-Anordnung zum spezifischen Nachweis von Mikroorganismen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE10061655A1 true DE10061655A1 (de) | 2002-06-20 |
Family
ID=7666693
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE10061655A Ceased DE10061655A1 (de) | 2000-12-11 | 2000-12-11 | In situ-Hybridisierungs-Anordnung zum spezifischen Nachweis von Mikroorganismen |
| DE50106653T Expired - Lifetime DE50106653D1 (de) | 2000-12-11 | 2001-12-11 | In situ-hybridisierungs-anordnung zum spezifischen nachweis von mikroorganismen |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE50106653T Expired - Lifetime DE50106653D1 (de) | 2000-12-11 | 2001-12-11 | In situ-hybridisierungs-anordnung zum spezifischen nachweis von mikroorganismen |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20040038270A1 (enExample) |
| EP (1) | EP1341897B1 (enExample) |
| JP (1) | JP2004523223A (enExample) |
| AT (1) | ATE298786T1 (enExample) |
| AU (1) | AU2002231689A1 (enExample) |
| CA (1) | CA2430894A1 (enExample) |
| DE (2) | DE10061655A1 (enExample) |
| WO (1) | WO2002048398A2 (enExample) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10323197A1 (de) * | 2003-05-22 | 2004-12-23 | Clondiag Chip Technologies Gmbh | Vorrichtung zur Halterung und Detektion von Substanzbibliotheken |
| DE102011004449A1 (de) * | 2011-02-21 | 2012-08-23 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Biopsieplättchen, Einbettkassette und Diagnosevorrichtung |
| CN106442020A (zh) * | 2016-11-18 | 2017-02-22 | 何耀 | 一种尿液取样检测装置 |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8062897B2 (en) | 2003-07-21 | 2011-11-22 | Aureon Laboratories, Inc. | Diagnostic histopathology using multiplex gene expression FISH |
| US6995020B2 (en) * | 2003-07-21 | 2006-02-07 | Aureon Laboratories, Inc. | Methods and compositions for the preparation and use of fixed-treated cell-lines and tissue in fluorescence in situ hybridization |
| JP4785449B2 (ja) * | 2005-07-21 | 2011-10-05 | 公益財団法人函館地域産業振興財団 | 培養併用蛍光インサイチューハイブリダイゼーション法による食品の微生物検査法 |
| US8481302B2 (en) * | 2008-11-03 | 2013-07-09 | General Electric Company | Total bacteria monitoring system |
| ES2387903B1 (es) * | 2010-07-16 | 2013-08-13 | María Albiñana Blanco | Procedimiento y kit para verificar, en una muestra de semen humano o animal, la existencia de espermatozoides fecundantes. |
| CN110672811A (zh) * | 2019-10-21 | 2020-01-10 | 远大可建科技有限公司 | 一种芯板内腔检测装置及其方法 |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3616265A (en) * | 1969-08-07 | 1971-10-26 | Smith Kline French Lab | Device for making a culture of microorganisms |
| FR2525233A1 (fr) * | 1982-04-14 | 1983-10-21 | Lille Inst Pasteur | Dispositif de culture microbiologique |
| DE8309876U1 (de) * | 1983-04-02 | 1983-12-22 | Biotest-Serum-Institut Gmbh, 6000 Frankfurt | Zweiphasige blutkulturflasche |
| EP0111664A1 (en) * | 1982-10-15 | 1984-06-27 | Life Technologies Inc. | Biphasic media culture apparatus |
| US4865988A (en) * | 1987-06-25 | 1989-09-12 | Sta Te Spa | Microbiological culture device |
| WO1993010211A1 (en) * | 1991-11-15 | 1993-05-27 | Aktieselskabet Nunc | A cell cultivating device |
| US5380492A (en) * | 1990-12-18 | 1995-01-10 | Seymour; Eugene H. | Sampling device and sample adequacy system |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3618265A (en) * | 1969-01-08 | 1971-11-09 | Remington Arms Co Inc | Finishing machine for metal surfaces |
| US4886741A (en) * | 1987-12-09 | 1989-12-12 | Microprobe Corporation | Use of volume exclusion agents for the enhancement of in situ hybridization |
| US5192503A (en) * | 1990-05-23 | 1993-03-09 | Mcgrath Charles M | Probe clip in situ assay apparatus |
| EP0497464A1 (en) * | 1991-01-31 | 1992-08-05 | Amoco Corporation | Rapid microbial diagnostics by in situ hybridization in aqueous suspension |
| US5128476A (en) * | 1991-02-20 | 1992-07-07 | The Midland Certified Reagent Company | Biotinylated oligonucleotides and reagents for preparing the same |
| DE4409705A1 (de) * | 1994-03-22 | 1995-09-28 | Boehringer Mannheim Gmbh | Vorrichtung zur Bearbeitung von Nukleinsäuren in Präparationen |
| US6022689A (en) * | 1995-04-07 | 2000-02-08 | University Of New Mexico | Situ hybridization slide processes |
| US5750340A (en) * | 1995-04-07 | 1998-05-12 | University Of New Mexico | In situ hybridization solution and process |
| US6007994A (en) * | 1995-12-22 | 1999-12-28 | Yale University | Multiparametric fluorescence in situ hybridization |
| WO2000065093A2 (en) * | 1999-04-22 | 2000-11-02 | Science And Technology Corporation | In situ hybridization methods for reducing the occurrence of false positives and for targeting multiple microorganisms |
-
2000
- 2000-12-11 DE DE10061655A patent/DE10061655A1/de not_active Ceased
-
2001
- 2001-12-11 AT AT01991823T patent/ATE298786T1/de active
- 2001-12-11 EP EP01991823A patent/EP1341897B1/de not_active Expired - Lifetime
- 2001-12-11 AU AU2002231689A patent/AU2002231689A1/en not_active Abandoned
- 2001-12-11 JP JP2002550112A patent/JP2004523223A/ja active Pending
- 2001-12-11 WO PCT/EP2001/014543 patent/WO2002048398A2/de not_active Ceased
- 2001-12-11 CA CA002430894A patent/CA2430894A1/en not_active Abandoned
- 2001-12-11 DE DE50106653T patent/DE50106653D1/de not_active Expired - Lifetime
-
2003
- 2003-06-10 US US10/458,775 patent/US20040038270A1/en not_active Abandoned
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3616265A (en) * | 1969-08-07 | 1971-10-26 | Smith Kline French Lab | Device for making a culture of microorganisms |
| FR2525233A1 (fr) * | 1982-04-14 | 1983-10-21 | Lille Inst Pasteur | Dispositif de culture microbiologique |
| EP0111664A1 (en) * | 1982-10-15 | 1984-06-27 | Life Technologies Inc. | Biphasic media culture apparatus |
| DE8309876U1 (de) * | 1983-04-02 | 1983-12-22 | Biotest-Serum-Institut Gmbh, 6000 Frankfurt | Zweiphasige blutkulturflasche |
| US4865988A (en) * | 1987-06-25 | 1989-09-12 | Sta Te Spa | Microbiological culture device |
| US5380492A (en) * | 1990-12-18 | 1995-01-10 | Seymour; Eugene H. | Sampling device and sample adequacy system |
| WO1993010211A1 (en) * | 1991-11-15 | 1993-05-27 | Aktieselskabet Nunc | A cell cultivating device |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10323197A1 (de) * | 2003-05-22 | 2004-12-23 | Clondiag Chip Technologies Gmbh | Vorrichtung zur Halterung und Detektion von Substanzbibliotheken |
| DE10323197B4 (de) * | 2003-05-22 | 2008-10-02 | Clondiag Chip Technologies Gmbh | Vorrichtung zur Halterung und Detektion von Substanzbibliotheken |
| DE102011004449A1 (de) * | 2011-02-21 | 2012-08-23 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Biopsieplättchen, Einbettkassette und Diagnosevorrichtung |
| DE102011004449B4 (de) | 2011-02-21 | 2019-01-24 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Biopsieplättchen, Einbettkassette und Diagnosevorrichtung |
| CN106442020A (zh) * | 2016-11-18 | 2017-02-22 | 何耀 | 一种尿液取样检测装置 |
Also Published As
| Publication number | Publication date |
|---|---|
| AU2002231689A1 (en) | 2002-06-24 |
| WO2002048398A3 (de) | 2003-05-15 |
| EP1341897B1 (de) | 2005-06-29 |
| ATE298786T1 (de) | 2005-07-15 |
| US20040038270A1 (en) | 2004-02-26 |
| DE50106653D1 (de) | 2005-08-04 |
| EP1341897A2 (de) | 2003-09-10 |
| WO2002048398A2 (de) | 2002-06-20 |
| CA2430894A1 (en) | 2002-06-20 |
| JP2004523223A (ja) | 2004-08-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE19732086C2 (de) | Verfahren zur quantitativen Bestimmung von Eubakterien | |
| DE69128425T2 (de) | Methoden und material für die trennung, konzentrierung und analyse von zellen | |
| EP1177319B1 (de) | Verfahren zum nachweisen von mikroorganismen in einer probe | |
| EP3548895A1 (de) | AUFBEREITUNG LEBENDIGER, MIKROBIELLER PROBEN UND MIKROORGANISMEN FÜR ANSCHLIEßENDE MASSENSPEKTROMETRISCHE MESSUNG UND AUSWERTUNG | |
| DE60118797T2 (de) | Verfahren zur detektion und messung der biomasse | |
| EP1341897B1 (de) | In situ-hybridisierungs-anordnung zum spezifischen nachweis von mikroorganismen | |
| DE60318723T2 (de) | Methode zur quantitativen bestimmung von polynukleotiden in einem gemisch | |
| DE19934510B4 (de) | Verfahren zum Nachweis von Mikroorganismen | |
| DE69835974T2 (de) | Nachweis und Quantifizierung von einen oder mehreren Nukleotidsequenzen durch Benutzung von räumlich lokalisierte Target Replikation | |
| EP1490507A1 (de) | Verfahren zum nachweis von mikroorganismen mittels in situ-hybridisierung und durchflusszytometrie | |
| AT512416B1 (de) | Anordnung und verfahren zum nachweis von mikroorganismen in einem kulturgefäss | |
| DE202012005258U1 (de) | Mittel zur schnellen quantitativen und qualitativen Bestimmung von Schmimmelpilzen und Hefen | |
| JP2002500020A (ja) | 嫌気性細菌の毒性化合物による阻止および殺害を迅速に評価する方法 | |
| EP1130115A2 (de) | Oligonukleotidsonden zum art- und/oder gattungsspezifischen Nachweis von das Pflanzenwachstum fördernden Bakterien | |
| EP1335991B1 (de) | Verfahren zum nachweis von protozoen der gattung naegleria | |
| EP1390541B1 (de) | Verfahren zum nachweis von gram-positiven bakterien | |
| DE10022304B4 (de) | Verfahren zur Identifizierung, Quantifizierung und Visualisierung von Mikroorganismen | |
| DE19936875C2 (de) | Verfahren zum Nachweisen von Mikroorganismen in einer Probe | |
| EP1490518B1 (de) | Verfahren zum nachweis und zur differenzierung des mycobacterium tuberculosis-komplex | |
| DE102020103961B4 (de) | Verfahren zum Nachweis von Mikroorganismen, korrespondierende Verwendung und Probenträger | |
| EP1723254A1 (de) | Qualitätssicherungssystem zum nachweis von mikroorganismen | |
| DE10160666A1 (de) | Verfahren zum spezifischen Schnellnachweis von Trinkwasser relevanten Bakterien | |
| DE102008002978A1 (de) | Verfahren zur Differenzierung von verschiedenen Spezies der Gattung Methylobakterium | |
| DE102005046361A1 (de) | Verfahren zur Unterscheidung von Salmonella-Serovaren |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OP8 | Request for examination as to paragraph 44 patent law | ||
| 8131 | Rejection |