CA2430894A1 - In situ hybridization system for specifically detecting microorganisms - Google Patents

In situ hybridization system for specifically detecting microorganisms Download PDF

Info

Publication number
CA2430894A1
CA2430894A1 CA002430894A CA2430894A CA2430894A1 CA 2430894 A1 CA2430894 A1 CA 2430894A1 CA 002430894 A CA002430894 A CA 002430894A CA 2430894 A CA2430894 A CA 2430894A CA 2430894 A1 CA2430894 A1 CA 2430894A1
Authority
CA
Canada
Prior art keywords
slide
hybridization
nucleic acid
sample
lid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002430894A
Other languages
English (en)
French (fr)
Inventor
Peter Muhlhahn
Jiri Snaidr
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Vermicon AG
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2430894A1 publication Critical patent/CA2430894A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6841In situ hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA002430894A 2000-12-11 2001-12-11 In situ hybridization system for specifically detecting microorganisms Abandoned CA2430894A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10061655.0 2000-12-11
DE10061655A DE10061655A1 (de) 2000-12-11 2000-12-11 In situ-Hybridisierungs-Anordnung zum spezifischen Nachweis von Mikroorganismen
PCT/EP2001/014543 WO2002048398A2 (de) 2000-12-11 2001-12-11 In situ-hybridisierungs-anordnung zum spezifischen nachweis von mikroorganismen

Publications (1)

Publication Number Publication Date
CA2430894A1 true CA2430894A1 (en) 2002-06-20

Family

ID=7666693

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002430894A Abandoned CA2430894A1 (en) 2000-12-11 2001-12-11 In situ hybridization system for specifically detecting microorganisms

Country Status (8)

Country Link
US (1) US20040038270A1 (enExample)
EP (1) EP1341897B1 (enExample)
JP (1) JP2004523223A (enExample)
AT (1) ATE298786T1 (enExample)
AU (1) AU2002231689A1 (enExample)
CA (1) CA2430894A1 (enExample)
DE (2) DE10061655A1 (enExample)
WO (1) WO2002048398A2 (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10323197B4 (de) * 2003-05-22 2008-10-02 Clondiag Chip Technologies Gmbh Vorrichtung zur Halterung und Detektion von Substanzbibliotheken
US8062897B2 (en) 2003-07-21 2011-11-22 Aureon Laboratories, Inc. Diagnostic histopathology using multiplex gene expression FISH
US6995020B2 (en) * 2003-07-21 2006-02-07 Aureon Laboratories, Inc. Methods and compositions for the preparation and use of fixed-treated cell-lines and tissue in fluorescence in situ hybridization
JP4785449B2 (ja) * 2005-07-21 2011-10-05 公益財団法人函館地域産業振興財団 培養併用蛍光インサイチューハイブリダイゼーション法による食品の微生物検査法
US8481302B2 (en) * 2008-11-03 2013-07-09 General Electric Company Total bacteria monitoring system
ES2387903B1 (es) * 2010-07-16 2013-08-13 María Albiñana Blanco Procedimiento y kit para verificar, en una muestra de semen humano o animal, la existencia de espermatozoides fecundantes.
DE102011004449B4 (de) * 2011-02-21 2019-01-24 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Biopsieplättchen, Einbettkassette und Diagnosevorrichtung
CN106442020B (zh) * 2016-11-18 2022-05-24 何耀 一种尿液取样检测装置
CN110672811A (zh) * 2019-10-21 2020-01-10 远大可建科技有限公司 一种芯板内腔检测装置及其方法

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3618265A (en) * 1969-01-08 1971-11-09 Remington Arms Co Inc Finishing machine for metal surfaces
US3616265A (en) * 1969-08-07 1971-10-26 Smith Kline French Lab Device for making a culture of microorganisms
FR2525233B1 (fr) * 1982-04-14 1985-12-06 Lille Inst Pasteur Dispositif de culture microbiologique
US4640895A (en) * 1982-10-15 1987-02-03 Gibco Division, The Mogul Corporation Biphasic media culture apparatus
DE8309876U1 (de) * 1983-04-02 1983-12-22 Biotest-Serum-Institut Gmbh, 6000 Frankfurt Zweiphasige blutkulturflasche
IT1205100B (it) * 1987-06-25 1989-03-10 Sta Te Spa Dispositivo per culture microbiologiche
US4886741A (en) * 1987-12-09 1989-12-12 Microprobe Corporation Use of volume exclusion agents for the enhancement of in situ hybridization
US5192503A (en) * 1990-05-23 1993-03-09 Mcgrath Charles M Probe clip in situ assay apparatus
US5380492A (en) * 1990-12-18 1995-01-10 Seymour; Eugene H. Sampling device and sample adequacy system
EP0497464A1 (en) * 1991-01-31 1992-08-05 Amoco Corporation Rapid microbial diagnostics by in situ hybridization in aqueous suspension
US5128476A (en) * 1991-02-20 1992-07-07 The Midland Certified Reagent Company Biotinylated oligonucleotides and reagents for preparing the same
DE667894T1 (de) * 1991-11-15 1996-09-12 Nunc As Vorrichtung zur kultivierung von zellen.
DE4409705A1 (de) * 1994-03-22 1995-09-28 Boehringer Mannheim Gmbh Vorrichtung zur Bearbeitung von Nukleinsäuren in Präparationen
US6022689A (en) * 1995-04-07 2000-02-08 University Of New Mexico Situ hybridization slide processes
US5750340A (en) * 1995-04-07 1998-05-12 University Of New Mexico In situ hybridization solution and process
US6007994A (en) * 1995-12-22 1999-12-28 Yale University Multiparametric fluorescence in situ hybridization
WO2000065093A2 (en) * 1999-04-22 2000-11-02 Science And Technology Corporation In situ hybridization methods for reducing the occurrence of false positives and for targeting multiple microorganisms

Also Published As

Publication number Publication date
AU2002231689A1 (en) 2002-06-24
WO2002048398A3 (de) 2003-05-15
EP1341897B1 (de) 2005-06-29
ATE298786T1 (de) 2005-07-15
DE10061655A1 (de) 2002-06-20
US20040038270A1 (en) 2004-02-26
DE50106653D1 (de) 2005-08-04
EP1341897A2 (de) 2003-09-10
WO2002048398A2 (de) 2002-06-20
JP2004523223A (ja) 2004-08-05

Similar Documents

Publication Publication Date Title
CN103328652B (zh) 测量用于测定未纯化的样品中细胞生活力的酶活性的方法
WO2000066789A3 (en) Polynucleotide matrix-based method of identifying microorganisms
EP0232085A2 (en) Campylobacter probe
AU773559B2 (en) Method for detecting microorganisms in a sample
CA2430894A1 (en) In situ hybridization system for specifically detecting microorganisms
US9567648B1 (en) Detection of foaming and bulking bacteria in wastewater
CN1995380B (zh) 一种检测和鉴定分枝杆菌菌种的方法及其专用试剂盒
EP1807533B1 (en) APPLICATIONS OF ISOLATED NUCLEIC ACID FRAGMENTS COMPRISING CpG ISLANDS
US20090136930A1 (en) Method for the identification of microorganisms by means of in situ hybridization and flow cytometry
JP2005532818A5 (enExample)
CA2479990A1 (en) Method for the identification of microorganisms by means of in situ hybridization and flow cytometry
US5948618A (en) Primer for gene amplification, method for nucleic acid discrimination with the use of the same, and nucleic acid discrimination kit
JPH0690798A (ja) 黄色ブドウ球菌検出用プローブ及び検出方法
CN120099202B (zh) 用于筛查木糖葡萄球菌的特异性分子标记、引物组及应用
US20050048524A1 (en) Molecular biological identification techniques for microorganisms
JPH1189597A (ja) 細菌の定量検出法
Bagwell et al. A DNA-DNA hybridization method for the detection and quantification of specific bacterial taxa in natural environments
Wang et al. Research Progress of Molecular Biology Technology in Soil Microbial Ecology
JP2004533252A (ja) 糸状細菌の特異的で迅速な検出方法
Buckley et al. Measurement of rRNA Abundance by Hybridization with Oligodeoxynucleotide Probes
JPH1066579A (ja) フェノール分解菌検出用オリゴヌクレオチド及びその使用

Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued