CZ81794A3 - Process for preparing gamma linoleic acid by the action of delta-6 desaturation - Google Patents

Process for preparing gamma linoleic acid by the action of delta-6 desaturation Download PDF

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Publication number
CZ81794A3
CZ81794A3 CZ94817A CZ81794A CZ81794A3 CZ 81794 A3 CZ81794 A3 CZ 81794A3 CZ 94817 A CZ94817 A CZ 94817A CZ 81794 A CZ81794 A CZ 81794A CZ 81794 A3 CZ81794 A3 CZ 81794A3
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Prior art keywords
nucleic acid
desaturase
isolated nucleic
gla
organism
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CZ94817A
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English (en)
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CZ285471B6 (cs
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L Thomas Terry
S Reddy Avutu
Michael Nuccio
L Freyssinet Georges
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Rhone Poulenc Agrochimie
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Publication of CZ81794A3 publication Critical patent/CZ81794A3/cs
Publication of CZ285471B6 publication Critical patent/CZ285471B6/cs

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/19—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water (1.14.19)
    • C12Y114/19003—Linoleoyl-CoA desaturase (1.14.19.3)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/82—Proteins from microorganisms
    • Y10S530/825—Bacteria

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Fats And Perfumes (AREA)

Claims (19)

1. Izolovaná nukleová kyselina kódující bakteriální Á6-desaturázu.
2. Nukleová kyselina podle nároku 1 obsahující nukleotidy ze SEQ. ID N0:3.
3. Izolovaná nukleová kyselina, která kóduje pro sekvenci aminokyseliny kódovanou nukleovou kyselinou podle nároku 1.
4. Izolovaná nukleová kyselina podle kteréhokoli z nároků
1 až 3, kde zmíněná nukleová kyselina Je obsažena ve vektoru.
5. Izolovaná nukleová kyselina podle nároku 4 operačně napojená na promotor a/nebo terminační signál schopný účinného vyjádření genového produktu zmíněné izolované nukleové kyseliny.
6. Izolovaná nukleová kyselina podle nároku 5, kde zmíněný promotor je promotor Αβ-desaturázy, promotor Anabaena karboxylázy, promotor helianthininu, promotor glycinu, promotor napinu nebo specifický tkáňový promotor helianthininu.
Τ'. Izolovaná nukleová kyselina podle nároku 5, kde terminační signál je Synechocystle terminační signál, terminační signál syntézy nopalinu nebo terminační signál semena.
8. Izolovaná nukleová kyselina podle7 kteréhokoli z nároků 1 až 7, kde izolovaná nukleová kyselina je obsažena ve transgenickém organismu.
9. Izolovaná nukleová kyselina podle nároku 8, kde transgenický organismus je bakterie, houba, rostlinná buňka nebo zvíře.
10. Způsob produkce rostliny se zvýšeným obsahem gamma linolenové kyseliny (GLA), vyznačující se tím, že obsahuje kroky:
(a) transformaci rostlinné buňky izolovanou nukleovou kyselinou podle kteréhokoli z nároků 1 až 7 a (b) regeneraci rostliny se zvýšeným obsahem GLA ze zmíněné rostlinné buňky.
11. Způsob podle nároku 10, vyznačující se tím, že rostlina je slunečnice, sojový bob, kukuřice, tabák, arašid nebo řepka olejka.
12. Způsob indukce tvorby gamma linolenové kyseliny (GLA) v organismu se sníženým obsahem nebo s chybějící GLA, vyznačující se tím, že se zmíněný organismus transformuje izolovanou nukleovou kyselinou podle kteréhokoli z nároků 1 až 7.
-2013. Způsob indukce tvorby gamma linolenové kyseliny (GLA) v organismu se sníženým obsahem nebo s chybějící GLA a linolovou kyselinou (LA), vyznačující se tím, že se zmíněný organismus transformuje izolovanou nukleovou kyselinou kódující bakteriální A6-desaturázu a izolovanou nukleovou kyselinou kódující Al2-desaturázu.
14. Způsob indukce tvorby gamroa linolenové kyseliny (GLA) v organismu se sníženým obsahem nebo s chybějící GLA a linolovou kyselinou (LA)* vyznačující se tím, že se zmíněný organismus transformuje alespoň jedním vektorem vyjádření obsahujícím izolovanou nukleovou kyselinu kódující bakteriální A6-desaturázu a izolovanou nukleovou kyselinu kódující Al2-desaturézu.
15. Způsob podle kteréhokoli z nároků 13 nebo 14, vyznačující se tím, že zmíněná izolovaná nukleová kyselina kódující 66-desaturázu obsahuje nukleotidy 317 až 1507 ze SEQ. ID N0:l.
16. Způsob indukce tvorby oktadekatetraeonové kyseliny v organismu se sníženým obsahem nebo chybějící gamma linolenovou kyselinou, vyznačující se tím, že se zmíněný organismus transformuje izolovanou nukleovou kyselinou podle kteréhokoli z nároků 1 až 7.
17. Způsob podle nároku 16, vyznačující se tím, že organismus je bakterie, houba, rostlina nebo zvíře.
18. Způsob produkce rostlinjr se zvýšenou odolností proti chladu, vyznačující se tím, že obsahuje:
(a) transformaci rostlinné buňky izolovanou nukleovou kyselinou podle kteréhokoli z nároků 1 až 7 a (b) regeneraci zmíněné rostliny se zlepšenou odolností proti chladu ze zmíněné rostlinné buňky.
19. Způsob podle nároku 18, vyznačující se tím, že rostlina je slunečnice, sojový bob, kukuřice, tabák, arašid nebo řepka olejka.
20. Izolovaná bakteriální A6-desaturéza.
21. Izolovaná bakteriální A6-desaturáza podle nároku 20, vyznačujíc í ac- léByr=£e má sekvenci aminokyselin ze SEQ. ID NO:2.
CZ94817A 1991-10-10 1992-10-13 Způsob výroby gama linolenové kyseliny účinkem delta-6 Desaturázy CZ285471B6 (cs)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US77447591A 1991-10-10 1991-10-10
US81791992A 1992-01-08 1992-01-08
PCT/US1992/008746 WO1993006712A1 (en) 1991-10-10 1992-10-13 Production of gamma linolenic acid by a δ6-desaturase

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CZ81794A3 true CZ81794A3 (en) 1995-09-13
CZ285471B6 CZ285471B6 (cs) 1999-08-11

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CZ94817A CZ285471B6 (cs) 1991-10-10 1992-10-13 Způsob výroby gama linolenové kyseliny účinkem delta-6 Desaturázy

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US (3) US5552306A (cs)
EP (1) EP0666918B1 (cs)
JP (1) JP3537434B2 (cs)
KR (1) KR100316068B1 (cs)
CN (2) CN1053469C (cs)
AT (1) ATE284961T1 (cs)
AU (1) AU667848B2 (cs)
BG (1) BG61791B1 (cs)
BR (1) BR9206613A (cs)
CA (1) CA2120629C (cs)
CZ (1) CZ285471B6 (cs)
DE (1) DE69233459T2 (cs)
DK (1) DK0666918T3 (cs)
ES (1) ES2231767T3 (cs)
HU (1) HU217328B (cs)
IL (1) IL103407A (cs)
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RU (1) RU2152996C2 (cs)
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