CY1124954T1 - Πλατφορμες διαλογης χημικα κωδικοποιημενων και χωρικα τοποθετημενων βιβλιοθηκων - Google Patents

Πλατφορμες διαλογης χημικα κωδικοποιημενων και χωρικα τοποθετημενων βιβλιοθηκων

Info

Publication number
CY1124954T1
CY1124954T1 CY20221100054T CY221100054T CY1124954T1 CY 1124954 T1 CY1124954 T1 CY 1124954T1 CY 20221100054 T CY20221100054 T CY 20221100054T CY 221100054 T CY221100054 T CY 221100054T CY 1124954 T1 CY1124954 T1 CY 1124954T1
Authority
CY
Cyprus
Prior art keywords
libraries
spotted
screening platforms
chemically encoded
screening
Prior art date
Application number
CY20221100054T
Other languages
Greek (el)
English (en)
Inventor
Jacob Berlin
Gregory Copeland
Kathleen ELISON
Hurik MURADYAN
Original Assignee
City of Hope
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=56789474&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CY1124954(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by City of Hope filed Critical City of Hope
Publication of CY1124954T1 publication Critical patent/CY1124954T1/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
    • C40B50/16—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support involving encoding steps
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B70/00—Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54346—Nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Nanotechnology (AREA)
  • Plant Pathology (AREA)
  • Structural Engineering (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Signal Processing For Digital Recording And Reproducing (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Peptides Or Proteins (AREA)
CY20221100054T 2015-02-24 2022-01-21 Πλατφορμες διαλογης χημικα κωδικοποιημενων και χωρικα τοποθετημενων βιβλιοθηκων CY1124954T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562120262P 2015-02-24 2015-02-24
PCT/US2016/019426 WO2016138184A1 (en) 2015-02-24 2016-02-24 Chemically encoded spatially addressed library screening platforms

Publications (1)

Publication Number Publication Date
CY1124954T1 true CY1124954T1 (el) 2023-01-05

Family

ID=56789474

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20221100054T CY1124954T1 (el) 2015-02-24 2022-01-21 Πλατφορμες διαλογης χημικα κωδικοποιημενων και χωρικα τοποθετημενων βιβλιοθηκων

Country Status (19)

Country Link
US (6) US10760181B2 (enExample)
EP (4) EP3262059B1 (enExample)
JP (2) JP6872501B2 (enExample)
AU (2) AU2016222755B2 (enExample)
CA (1) CA2976946C (enExample)
CY (1) CY1124954T1 (enExample)
DK (3) DK3262059T3 (enExample)
ES (3) ES2954467T3 (enExample)
FI (2) FI3650458T3 (enExample)
HR (3) HRP20231536T1 (enExample)
HU (3) HUE063406T2 (enExample)
LT (3) LT3650458T (enExample)
NZ (1) NZ734644A (enExample)
PL (3) PL3262059T3 (enExample)
PT (3) PT3650459T (enExample)
RS (3) RS62847B1 (enExample)
SI (3) SI3650458T1 (enExample)
SM (3) SMT202300430T1 (enExample)
WO (1) WO2016138184A1 (enExample)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2622112B2 (ja) 1986-09-26 1997-06-18 キヤノン株式会社 画像処理システム
WO2009069203A1 (ja) * 2007-11-28 2009-06-04 Glory Ltd. チケット処理装置、チケット処理システム及びチケット処理方法
US10760181B2 (en) * 2015-02-24 2020-09-01 City Of Hope Chemically encoded spatially addressed library screening platforms
EP3284851B1 (en) * 2015-04-14 2020-12-23 Hitgen Inc. Method for solid-phase synthesis of dna-encoded chemical library
CN110249050B (zh) * 2016-11-08 2023-08-18 南纳治疗有限公司 无标签编码化合物文库
US11084037B2 (en) 2017-09-25 2021-08-10 Plexium, Inc. Oligonucleotide encoded chemical libraries
US20210355483A1 (en) 2017-10-31 2021-11-18 Encodia, Inc. Methods and kits using nucleic acid encoding and/or label
AU2020361681A1 (en) 2019-10-10 2022-05-05 1859, Inc. Methods and systems for microfluidic screening
US12577702B2 (en) 2020-01-28 2026-03-17 Plexium, Inc. Oligonucleotide encoded chemical libraries, related systems, devices, and methods for detecting, analyzing, quantifying, and testing biologics/genetics
WO2022170034A1 (en) * 2021-02-05 2022-08-11 Affinergy, Llc Rapid assay for apol1 g0 protein
US20250368840A1 (en) * 2021-08-27 2025-12-04 National University Corporation Kobe University Core-shell template molecule/particle for technique for high-yield formation of pores on substrate

Family Cites Families (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4672040A (en) * 1983-05-12 1987-06-09 Advanced Magnetics, Inc. Magnetic particles for use in separations
US5235033A (en) 1985-03-15 1993-08-10 Anti-Gene Development Group Alpha-morpholino ribonucleoside derivatives and polymers thereof
US5034506A (en) 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
US4946778A (en) 1987-09-21 1990-08-07 Genex Corporation Single polypeptide chain binding molecules
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
EP0604552B1 (en) 1991-09-18 1997-02-12 Affymax Technologies N.V. Method of synthesizing diverse collections of oligomers
US5573905A (en) 1992-03-30 1996-11-12 The Scripps Research Institute Encoded combinatorial chemical libraries
US5565324A (en) 1992-10-01 1996-10-15 The Trustees Of Columbia University In The City Of New York Complex combinatorial chemical libraries encoded with tags
WO1994013623A1 (en) 1992-12-11 1994-06-23 Chiron Corporation Synthesis of encoded polymers
CA2163637C (en) * 1993-05-27 2008-05-06 Michal Lebl Topologically segregated, encoded solid phase libraries
NZ268292A (en) * 1993-06-21 1997-09-22 Selectide Corp Solid phase peptide synthesis and supports therefor
US6087186A (en) 1993-07-16 2000-07-11 Irori Methods and apparatus for synthesizing labeled combinatorial chemistry libraries
JPH09508353A (ja) * 1993-11-02 1997-08-26 アフィマックス テクノロジーズ ナムローゼ フェンノートシャップ 分子多様性の合成及びスクリーニング
US6936477B2 (en) 1994-04-13 2005-08-30 The Trustees Of Columbia University In The City Of New York Complex combinatorial chemical libraries encoded with tags
US7615368B1 (en) 1994-06-17 2009-11-10 The Board Of Trustees Of The Leland Stanford Junior University Microarrays of polypeptides
US5807522A (en) 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US5830645A (en) 1994-12-09 1998-11-03 The Regents Of The University Of California Comparative fluorescence hybridization to nucleic acid arrays
US5846839A (en) 1995-12-22 1998-12-08 Glaxo Group Limited Methods for hard-tagging an encoded synthetic library
US6251691B1 (en) 1996-04-25 2001-06-26 Bioarray Solutions, Llc Light-controlled electrokinetic assembly of particles near surfaces
IL127938A (en) * 1996-07-08 2002-09-12 Burstein Lab Inc A device with a main signal fixed for diagnostic applications and a test method
DE69706187T2 (de) * 1996-11-06 2002-04-11 Sequenom, Inc. Zusammensetzungen und verfahren zur immobilisierung von nukleinsäure auf festträgern
US7060431B2 (en) 1998-06-24 2006-06-13 Illumina, Inc. Method of making and decoding of array sensors with microspheres
US6429027B1 (en) * 1998-12-28 2002-08-06 Illumina, Inc. Composite arrays utilizing microspheres
US20020150909A1 (en) * 1999-02-09 2002-10-17 Stuelpnagel John R. Automated information processing in randomly ordered arrays
US6824987B1 (en) 1999-05-11 2004-11-30 President And Fellows Of Harvard College Small molecule printing
US7932213B2 (en) 1999-05-11 2011-04-26 President And Fellows Of Harvard College Small molecule printing
US6544732B1 (en) * 1999-05-20 2003-04-08 Illumina, Inc. Encoding and decoding of array sensors utilizing nanocrystals
US7160735B2 (en) 2000-04-28 2007-01-09 Monogram Biosciences, Inc. Tagged microparticle compositions and methods
JP3899831B2 (ja) 2001-03-02 2007-03-28 株式会社日立製作所 生化学センサ及びこれを用いた生化学検査装置
US20060040286A1 (en) 2001-03-28 2006-02-23 Nanosphere, Inc. Bio-barcode based detection of target analytes
US20030027221A1 (en) 2001-04-06 2003-02-06 Scott Melissa E. High-throughput screening assays by encapsulation
US20030228619A1 (en) 2002-06-10 2003-12-11 Xenoport, Inc. Peptide nucleic acids as tags in encoded libraries
CA2565967A1 (en) 2004-06-10 2005-12-29 Perkinelmer Las, Inc. Multiplexing assays for analyte detection
US20060134697A1 (en) * 2004-10-28 2006-06-22 The Regents Of The University Of California Method of preparing coded compound libraries
AU2006336290A1 (en) * 2005-09-16 2007-07-26 The Regents Of The University Of California A colorimetric bio-barcode amplification assay for analyte detection
EP2396409A2 (en) 2009-02-10 2011-12-21 Idera Pharmaceuticals, Inc. Synthetic rna-based agonists of tlr7
US9172561B2 (en) 2009-07-29 2015-10-27 Qualcomm Incorporated Adaptive transmissions in coordinated multiple point communications
WO2011014879A2 (en) 2009-07-31 2011-02-03 Prognosys Biosciences, Inc. Assay tools and methods of use
WO2011143583A1 (en) * 2010-05-13 2011-11-17 Illumina, Inc. Binding assays for markers
CN104781276A (zh) 2012-08-14 2015-07-15 安吉奥开米公司 肽-树枝状聚合物结合物及其用途
CN104620093B (zh) * 2012-08-23 2018-04-20 干细胞技术公司 用于快速可逆生物分子标记的组合物和方法
US10760181B2 (en) 2015-02-24 2020-09-01 City Of Hope Chemically encoded spatially addressed library screening platforms

Also Published As

Publication number Publication date
SI3650458T1 (sl) 2023-11-30
AU2021200399B2 (en) 2023-04-20
US20220341059A1 (en) 2022-10-27
US12624476B2 (en) 2026-05-12
SI3262059T1 (sl) 2022-04-29
EP4276059A3 (en) 2024-05-01
HRP20220093T1 (hr) 2022-04-15
RS62847B1 (sr) 2022-02-28
EP3650458A1 (en) 2020-05-13
US10767278B2 (en) 2020-09-08
SMT202300275T1 (it) 2023-11-13
PT3262059T (pt) 2022-01-27
ES2954467T3 (es) 2023-11-22
AU2016222755A1 (en) 2017-08-31
US11015266B2 (en) 2021-05-25
US11015265B2 (en) 2021-05-25
RS64567B1 (sr) 2023-10-31
US20200378031A1 (en) 2020-12-03
AU2016222755B2 (en) 2021-02-18
CA2976946C (en) 2023-09-19
US20210324538A1 (en) 2021-10-21
DK3650459T3 (da) 2023-12-11
JP7109618B2 (ja) 2022-07-29
EP3262059A4 (en) 2018-12-05
DK3262059T3 (da) 2022-01-31
RS64899B1 (sr) 2023-12-29
SI3650459T1 (sl) 2024-04-30
PL3650458T3 (pl) 2023-11-06
FI3650458T3 (fi) 2023-08-21
LT3650459T (lt) 2023-12-27
AU2021200399A1 (en) 2021-03-18
DK3650458T5 (da) 2024-08-26
LT3262059T (lt) 2022-02-25
PT3650459T (pt) 2023-12-06
US20180245241A1 (en) 2018-08-30
SMT202300430T1 (it) 2024-01-10
US20190390367A1 (en) 2019-12-26
EP3650459A1 (en) 2020-05-13
LT3650458T (lt) 2023-10-10
PT3650458T (pt) 2023-08-31
DK3650458T3 (da) 2023-09-04
ES2904997T3 (es) 2022-04-06
NZ734644A (en) 2023-02-24
US10760181B2 (en) 2020-09-01
EP3262059B1 (en) 2022-01-05
JP2018505700A (ja) 2018-03-01
JP6872501B2 (ja) 2021-05-19
HUE063406T2 (hu) 2024-01-28
PL3262059T3 (pl) 2022-02-21
EP4276059A2 (en) 2023-11-15
US20200378032A1 (en) 2020-12-03
ES2965764T3 (es) 2024-04-16
WO2016138184A1 (en) 2016-09-01
JP2021106607A (ja) 2021-07-29
JP7109618B6 (ja) 2022-08-22
EP3650458B1 (en) 2023-08-09
DK3650459T5 (da) 2024-08-26
EP3650459B1 (en) 2023-09-06
CA2976946A1 (en) 2016-09-01
NZ772386A (en) 2024-10-25
SMT202200039T1 (it) 2022-03-21
FI3650459T3 (fi) 2023-11-28
HRP20230928T1 (hr) 2023-11-24
HRP20231536T1 (hr) 2024-03-15
HUE064689T2 (hu) 2024-04-28
PL3650459T3 (pl) 2024-02-26
EP3262059A1 (en) 2018-01-03
HUE058813T2 (hu) 2022-09-28

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