CY1122030T1 - Μεθοδος εκτιμησης της ποσοτητας μεθυλιωμενου τοπου σε ενα δειγμα - Google Patents
Μεθοδος εκτιμησης της ποσοτητας μεθυλιωμενου τοπου σε ενα δειγμαInfo
- Publication number
- CY1122030T1 CY1122030T1 CY20181101045T CY181101045T CY1122030T1 CY 1122030 T1 CY1122030 T1 CY 1122030T1 CY 20181101045 T CY20181101045 T CY 20181101045T CY 181101045 T CY181101045 T CY 181101045T CY 1122030 T1 CY1122030 T1 CY 1122030T1
- Authority
- CY
- Cyprus
- Prior art keywords
- amount
- estimating
- sequence
- methylated
- sample
- Prior art date
Links
- 239000012634 fragment Substances 0.000 abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
- C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/154—Methylation markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Hospice & Palliative Care (AREA)
- Oncology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Παρέχεται μια μέθοδος εκτίμησης της ποσότητας ενός μεθυλιωμένου τόπου. Σε ορισμένες υλοποιήσεις η μέθοδος περιλαμβάνει: πέψη ενός δείγματος νουκλεϊκού οξέος που περιέχει τόσο μη μεθυλιωμένα όσο και μεθυλιωμένα αντίγραφα γονιδιωματικού τόπου με ένα μέλος οικογένειας MspJI για να παραχθεί ένας πληθυσμός θραυσμάτων που κυμαίνονται από 20-40 νουκλεοτίδια σε μήκος, ομοιοπολική σύνδεση αλληλουχίας προσαρμογέα Α και αλληλουχίας προσαρμογέα Β στα αντίστοιχα άκρα ενός θραύσματος στόχου της αλληλουχίας Χ και ποσοτικοποίηση της ποσότητας προϊόντων ομοιοπολικής σύνδεσης με τον τύπο ΑΧΒ. Παρέχεται επίσης ένα κιτ για την εκτέλεση της μεθόδου.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201462037057P | 2014-08-13 | 2014-08-13 | |
PCT/IB2015/055799 WO2016024182A1 (en) | 2014-08-13 | 2015-07-31 | Method of estimating the amount of a methylated locus in a sample |
Publications (1)
Publication Number | Publication Date |
---|---|
CY1122030T1 true CY1122030T1 (el) | 2020-10-14 |
Family
ID=53783792
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CY20181101045T CY1122030T1 (el) | 2014-08-13 | 2018-10-11 | Μεθοδος εκτιμησης της ποσοτητας μεθυλιωμενου τοπου σε ενα δειγμα |
Country Status (8)
Country | Link |
---|---|
US (2) | US10174383B2 (el) |
EP (1) | EP3180441B1 (el) |
CN (1) | CN107109468B (el) |
AU (1) | AU2015302946B2 (el) |
CA (1) | CA2956883C (el) |
CY (1) | CY1122030T1 (el) |
ES (1) | ES2690583T3 (el) |
WO (1) | WO2016024182A1 (el) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2520765A (en) * | 2013-12-02 | 2015-06-03 | Vanadis Diagnostics Ab | Multiplex detection of nucleic acids |
EP3350342B1 (en) * | 2015-09-18 | 2020-02-19 | Vanadis Diagnostics | Probe set for analyzing a dna sample and method for using the same |
IL265451B (en) * | 2019-03-18 | 2020-01-30 | Frumkin Dan | Methods and systems for the detection of methylation changes in DNA samples |
CN110484621B (zh) * | 2019-08-12 | 2020-04-28 | 江苏吉睿生物技术研究院有限公司 | 一种肝癌早期预警的方法 |
CN116064797B (zh) * | 2022-08-29 | 2023-10-20 | 广州达健生物科技有限公司 | 一种子宫内膜癌基因甲基化水平检测试剂及其应用 |
Family Cites Families (31)
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US5981179A (en) | 1991-11-14 | 1999-11-09 | Digene Diagnostics, Inc. | Continuous amplification reaction |
US5604097A (en) | 1994-10-13 | 1997-02-18 | Spectragen, Inc. | Methods for sorting polynucleotides using oligonucleotide tags |
US6458530B1 (en) | 1996-04-04 | 2002-10-01 | Affymetrix Inc. | Selecting tag nucleic acids |
US5871917A (en) | 1996-05-31 | 1999-02-16 | North Shore University Hospital Research Corp. | Identification of differentially methylated and mutated nucleic acids |
ATE545710T1 (de) | 1997-04-01 | 2012-03-15 | Illumina Cambridge Ltd | Verfahren zur vervielfältigung von nukleinsäuren |
US5948902A (en) | 1997-11-20 | 1999-09-07 | South Alabama Medical Science Foundation | Antisense oligonucleotides to human serine/threonine protein phosphatase genes |
DE19754482A1 (de) * | 1997-11-27 | 1999-07-01 | Epigenomics Gmbh | Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke |
AR021833A1 (es) | 1998-09-30 | 2002-08-07 | Applied Research Systems | Metodos de amplificacion y secuenciacion de acido nucleico |
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DE10154318A1 (de) | 2001-10-26 | 2003-05-15 | Epigenomics Ag | Verfahren zur Analyse genomischer Methylierungsmuster |
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US20050233340A1 (en) | 2004-04-20 | 2005-10-20 | Barrett Michael T | Methods and compositions for assessing CpG methylation |
WO2006088978A1 (en) | 2005-02-16 | 2006-08-24 | Epigenomics, Inc. | Method for determining the methylation pattern of a polynucleic acid |
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AU2008268508A1 (en) | 2007-06-22 | 2008-12-31 | The Trustees Of Columbia University In The City Of New York | Specific amplification of tumor specific DNA sequences |
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US8476013B2 (en) | 2008-09-16 | 2013-07-02 | Sequenom, Inc. | Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses |
CN102264900A (zh) * | 2008-12-23 | 2011-11-30 | 新英格兰生物实验室公司 | 用于切割修饰的dna的组合物、方法和相关用途 |
WO2010113088A1 (en) | 2009-03-31 | 2010-10-07 | Centre Hospitalier Universitaire Vaudois | Methylation ligation-dependent macroarray (mlm) |
US9260745B2 (en) * | 2010-01-19 | 2016-02-16 | Verinata Health, Inc. | Detecting and classifying copy number variation |
DK2529032T3 (en) * | 2010-01-26 | 2017-05-01 | Nipd Genetics Ltd | METHODS AND COMPOSITIONS FOR NON-INVASIVE PRE-NATIONAL DIAGNOSTICATION OF Fetal ANEUPLOIDITIES |
CN103069006A (zh) * | 2010-07-23 | 2013-04-24 | 艾索特里克斯遗传实验室有限责任公司 | 区别表达的胚胎或母源基因组区的鉴定及其用途 |
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-
2015
- 2015-07-30 US US14/814,412 patent/US10174383B2/en active Active
- 2015-07-31 WO PCT/IB2015/055799 patent/WO2016024182A1/en active Application Filing
- 2015-07-31 EP EP15747564.1A patent/EP3180441B1/en active Active
- 2015-07-31 CA CA2956883A patent/CA2956883C/en active Active
- 2015-07-31 ES ES15747564.1T patent/ES2690583T3/es active Active
- 2015-07-31 AU AU2015302946A patent/AU2015302946B2/en active Active
- 2015-07-31 CN CN201580046389.6A patent/CN107109468B/zh active Active
-
2018
- 2018-10-11 CY CY20181101045T patent/CY1122030T1/el unknown
- 2018-12-04 US US16/209,785 patent/US10876169B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
ES2690583T3 (es) | 2018-11-21 |
EP3180441A1 (en) | 2017-06-21 |
EP3180441B1 (en) | 2018-09-26 |
CA2956883C (en) | 2023-03-07 |
AU2015302946A1 (en) | 2017-02-23 |
US20160047002A1 (en) | 2016-02-18 |
AU2015302946B2 (en) | 2017-12-21 |
CA2956883A1 (en) | 2016-02-18 |
US10174383B2 (en) | 2019-01-08 |
CN107109468B (zh) | 2020-09-08 |
WO2016024182A1 (en) | 2016-02-18 |
US10876169B2 (en) | 2020-12-29 |
US20190106753A1 (en) | 2019-04-11 |
CN107109468A (zh) | 2017-08-29 |
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