BR112016028442A2 - processos para detectar a metilação de citosina de ácido nucleico alvo de interesse e/ou diagnosticar ou monitorar estado de saúde em paciente e kit - Google Patents

processos para detectar a metilação de citosina de ácido nucleico alvo de interesse e/ou diagnosticar ou monitorar estado de saúde em paciente e kit

Info

Publication number
BR112016028442A2
BR112016028442A2 BR112016028442A BR112016028442A BR112016028442A2 BR 112016028442 A2 BR112016028442 A2 BR 112016028442A2 BR 112016028442 A BR112016028442 A BR 112016028442A BR 112016028442 A BR112016028442 A BR 112016028442A BR 112016028442 A2 BR112016028442 A2 BR 112016028442A2
Authority
BR
Brazil
Prior art keywords
nucleic acid
dna
diagnosing
kit
interest
Prior art date
Application number
BR112016028442A
Other languages
English (en)
Inventor
Baker Rohan
Pedersen Susanne
Original Assignee
Clinical Genomics Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2014902155A external-priority patent/AU2014902155A0/en
Application filed by Clinical Genomics Pty Ltd filed Critical Clinical Genomics Pty Ltd
Publication of BR112016028442A2 publication Critical patent/BR112016028442A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/10—Characterised by chemical treatment
    • C12Q2523/125—Bisulfite(s)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00—Nucleic acid detection characterised by assay method
    • C12Q2561/101—Taqman
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

a presente invenção refere-se de forma geral à avaliação da metilação de ácidos nucleicos, em particular metilação de dna e rna. mais particularmente, a presente invenção refere-se a um processo de avaliação de forma qualitativa ou quantitativa, com maior sensibilidade, da metilação de citosina de dna ou rna parcialmente metilado. o processo da presente invenção é útil em uma faixa de aplicações que incluem, mas não estão limitadas ao diagnóstico de estados de saúde ou ao monitoramento de fenótipos do desenvolvimento que são caracterizados por alterações na metilação do dna ou do rna.
BR112016028442A 2014-06-05 2015-06-01 processos para detectar a metilação de citosina de ácido nucleico alvo de interesse e/ou diagnosticar ou monitorar estado de saúde em paciente e kit BR112016028442A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AU2014902155A AU2014902155A0 (en) 2014-06-05 Method for Methylation Analysis
PCT/AU2015/050297 WO2015184498A2 (en) 2014-06-05 2015-06-01 Method for methylation analysis

Publications (1)

Publication Number Publication Date
BR112016028442A2 true BR112016028442A2 (pt) 2017-10-24

Family

ID=54767512

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112016028442A BR112016028442A2 (pt) 2014-06-05 2015-06-01 processos para detectar a metilação de citosina de ácido nucleico alvo de interesse e/ou diagnosticar ou monitorar estado de saúde em paciente e kit

Country Status (15)

Country Link
US (1) US10590468B2 (pt)
EP (1) EP3152332B1 (pt)
JP (1) JP6810612B2 (pt)
KR (1) KR20170012552A (pt)
CN (1) CN106687600B (pt)
AU (1) AU2015271641B2 (pt)
BR (1) BR112016028442A2 (pt)
CA (1) CA2950953A1 (pt)
DK (1) DK3152332T3 (pt)
IL (1) IL249331A0 (pt)
MX (1) MX2016016062A (pt)
PH (1) PH12016502411A1 (pt)
SG (1) SG11201610113YA (pt)
WO (1) WO2015184498A2 (pt)
ZA (1) ZA201608367B (pt)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2018256387A1 (en) 2017-04-19 2019-10-31 Singlera Genomics, Inc. Compositions and methods for library construction and sequence analysis
KR101959538B1 (ko) * 2017-05-29 2019-03-20 대한민국 CpG 아일랜드 예측 및 프라이머 제작을 위한 파이프라인 방법
AU2018333844A1 (en) * 2017-09-15 2020-04-09 Clinical Genomics Pty Ltd Method for methylation analysis
JP7125782B2 (ja) * 2018-02-22 2022-08-25 国立大学法人大阪大学 Ｒｎａ修飾を利用した分析・診断法
WO2019223520A1 (zh) * 2018-05-22 2019-11-28 广州市康立明生物科技有限责任公司 肿瘤标志物、甲基化检测试剂、试剂盒及其应用
CN110511999A (zh) * 2018-05-22 2019-11-29 广州市康立明生物科技有限责任公司 肿瘤标志物、甲基化试剂、试剂盒及其应用
CN110512001A (zh) * 2018-05-22 2019-11-29 广州市康立明生物科技有限责任公司 肿瘤标志物、甲基化试剂、试剂盒及其应用
CN110527708A (zh) * 2019-07-11 2019-12-03 武汉伯远生物科技有限公司 一种区分dna中5-甲基化胞嘧啶和5-羟甲基化胞嘧啶的方法
CN110331142B (zh) * 2019-07-11 2021-12-21 广州康立明生物科技股份有限公司 一种多基因联合检测试剂

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6331393B1 (en) * 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis
WO2005042713A2 (en) * 2003-10-28 2005-05-12 The Johns Hopkins University Quantitative multiplex methylation-specific pcr
WO2008135512A2 (en) * 2007-05-02 2008-11-13 Jerzy Paszkowski Dna amplification method
BR112014028122B1 (pt) * 2012-05-11 2022-08-23 Commonwealth Scientific And Industrial Research Organisation Método para triar o princípio ou a predisposição ao princípio de neoplasma no intestino grosso ou para monitorar neoplasma no intestino grosso em indivíduo
CN104520442B (zh) * 2012-05-22 2018-05-01 约翰霍普金斯大学 定量多重甲基化特异性PCR方法-cMethDNA、试剂及其用途
US20150141256A1 (en) * 2013-07-29 2015-05-21 Roche Nimblegen, Inc. Compositions and methods for bisulfite converted sequence capture

Also Published As

Publication number Publication date
PH12016502411A1 (en) 2017-02-27
US10590468B2 (en) 2020-03-17
AU2015271641A1 (en) 2016-12-22
US20170145485A1 (en) 2017-05-25
KR20170012552A (ko) 2017-02-02
EP3152332B1 (en) 2021-03-03
SG11201610113YA (en) 2017-01-27
CA2950953A1 (en) 2015-12-10
JP6810612B2 (ja) 2021-01-06
AU2015271641B2 (en) 2021-04-08
IL249331A0 (en) 2017-02-28
MX2016016062A (es) 2017-03-16
WO2015184498A2 (en) 2015-12-10
ZA201608367B (en) 2019-05-29
WO2015184498A3 (en) 2016-03-03
CN106687600B (zh) 2021-08-17
EP3152332A4 (en) 2018-01-10
JP2017517262A (ja) 2017-06-29
CN106687600A (zh) 2017-05-17
EP3152332A2 (en) 2017-04-12
DK3152332T3 (da) 2021-03-29

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Legal Events

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B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements