BR112016006312A2 - método e kit para determinar a presença ou ausência de staphylococcus aureus resistente à meticilina em uma amostra biológica - Google Patents

método e kit para determinar a presença ou ausência de staphylococcus aureus resistente à meticilina em uma amostra biológica

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Publication number
BR112016006312A2
BR112016006312A2 BR112016006312A BR112016006312A BR112016006312A2 BR 112016006312 A2 BR112016006312 A2 BR 112016006312A2 BR 112016006312 A BR112016006312 A BR 112016006312A BR 112016006312 A BR112016006312 A BR 112016006312A BR 112016006312 A2 BR112016006312 A2 BR 112016006312A2
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BR
Brazil
Prior art keywords
determining
staphylococcus aureus
resistant staphylococcus
methicillin
kit
Prior art date
Application number
BR112016006312A
Other languages
English (en)
Other versions
BR112016006312B1 (pt
Inventor
Vincent Heather
Mai Huong
Nair Lakshmi
Exner Maurice
Aye Michael
Tabb Michelle
Original Assignee
Quest Diagnostics Invest Inc
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Filing date
Publication date
Application filed by Quest Diagnostics Invest Inc filed Critical Quest Diagnostics Invest Inc
Publication of BR112016006312A2 publication Critical patent/BR112016006312A2/pt
Publication of BR112016006312B1 publication Critical patent/BR112016006312B1/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/101—Interaction between at least two labels
    • C12Q2565/1015—Interaction between at least two labels labels being on the same oligonucleotide
    • C—CHEMISTRY; METALLURGY
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/112—Disease subtyping, staging or classification
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6432—Quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

são descritos métodos para identificar staphylococcus aureus resistente à meticilina (mrsa) em uma amostra em que os métodos envolvem detectar uma sequência de ácido nucleico específica para s. aureus, meca e mecc na amostra. kits para determinar a presença de mrsa em uma amostra também são providos.
BR112016006312-0A 2013-09-23 2014-09-22 Método e kit para determinar a presença ou ausência de staphylococcus aureus resistente à meticilina em uma amostra biológica BR112016006312B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361881234P 2013-09-23 2013-09-23
US61/881234 2013-09-23
PCT/US2014/056777 WO2015042526A1 (en) 2013-09-23 2014-09-22 Detection of methicillin-resistant staphylococcus aureus in biological samples

Publications (2)

Publication Number Publication Date
BR112016006312A2 true BR112016006312A2 (pt) 2017-09-26
BR112016006312B1 BR112016006312B1 (pt) 2023-04-18

Family

ID=52689504

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112016006312-0A BR112016006312B1 (pt) 2013-09-23 2014-09-22 Método e kit para determinar a presença ou ausência de staphylococcus aureus resistente à meticilina em uma amostra biológica

Country Status (7)

Country Link
US (4) US10407739B2 (pt)
EP (1) EP3049537B1 (pt)
JP (2) JP6659533B2 (pt)
CN (1) CN105793436A (pt)
BR (1) BR112016006312B1 (pt)
CA (1) CA2925168C (pt)
WO (1) WO2015042526A1 (pt)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10407739B2 (en) 2013-09-23 2019-09-10 Quest Diagnostics Investments Incorporated Detection of methicillin-resistant Staphylococcus aureus in biological samples
CN107312833B (zh) * 2017-06-01 2020-05-15 厦门安普利生物工程有限公司 用于检测人类brca1基因突变的lsp引物及试剂盒
CN108034735B (zh) * 2017-11-27 2021-02-23 宁波市医疗中心李惠利医院 一种关节假体感染诊断试剂盒
JP6954138B2 (ja) * 2018-01-15 2021-10-27 株式会社デンソー 蓄熱装置
CN110734988A (zh) * 2018-07-20 2020-01-31 上海仁度生物科技有限公司 一种耐甲氧西林金黄色葡萄球菌（mrsa）核酸恒温扩增方法
CN109187508B (zh) * 2018-07-27 2020-12-25 湖南人文科技学院 基于电化学发光特性传感的转肽酶检测方法
CN109355403B (zh) * 2018-08-10 2021-03-30 华南理工大学 一种psr检测耐甲氧西林金葡菌的引物、试剂盒与方法
EP4081666A2 (en) * 2019-12-27 2022-11-02 F. Hoffmann-La Roche AG Compositions and methods for detecting methicillin-resistant staphylococcus aureus
CN112195225A (zh) * 2020-10-14 2021-01-08 南通大学附属医院 基于耐药基因MecA检测耐甲氧西林葡萄球菌的方法
JPWO2022092013A1 (pt) * 2020-10-30 2022-05-05
WO2023135998A1 (ja) * 2022-01-14 2023-07-20 株式会社日立製作所 構造多型変異検出法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6117986A (en) 1998-06-10 2000-09-12 Intergen Company, L.P. Pyrimidines linked to a quencher
US7888075B2 (en) 2007-07-31 2011-02-15 Quest Diagnostics Investments Incorporated Detection of methicillin-resistant and methicillin-sensitive Staphylococcus aureus in biological samples
EP2116614A1 (en) 2008-05-06 2009-11-11 Qiagen GmbH Simultaneous detection of multiple nucleic acid sequences in a reaction
WO2010121298A1 (en) 2009-04-20 2010-10-28 Human Genetic Signatures Pty Ltd Detection of staphylococcus aureus
CA2793660C (en) * 2010-03-19 2020-12-22 The Translational Genomics Research Institute Methods, kits and compositions for detection of mrsa
WO2012024642A1 (en) * 2010-08-20 2012-02-23 Life Technologies Corporation Quantitative real-time pcr assay using fret dual-labeled primers
US20120165229A1 (en) 2010-12-07 2012-06-28 Intelligent Medical Devices, Inc. Optimized probes and primers and methods of using same for the detection, screening, isolation and sequencing of mrsa, mssa, staphylococcus markers, and the antibiotic resistance gene mec a
ES2537189T3 (es) 2011-05-24 2015-06-03 Elitech Holding B.V. Detección de estafilococos áureos resistentes a la meticilina
CN104169437B (zh) 2011-12-23 2017-07-11 生物梅里埃公司 甲氧西林抗性金黄色葡萄球菌的mecA变体菌株的检测
EP2617836A1 (en) 2012-01-23 2013-07-24 Steffen Mergemeier Method for detecting a methicillin resistant coagulase positive Staphylococcus aureus strain
US10407739B2 (en) * 2013-09-23 2019-09-10 Quest Diagnostics Investments Incorporated Detection of methicillin-resistant Staphylococcus aureus in biological samples

Also Published As

Publication number Publication date
BR112016006312B1 (pt) 2023-04-18
US10385408B2 (en) 2019-08-20
US11674189B2 (en) 2023-06-13
US20160230216A1 (en) 2016-08-11
CA2925168A1 (en) 2015-03-26
JP6659533B2 (ja) 2020-03-04
US20240026467A1 (en) 2024-01-25
CN105793436A (zh) 2016-07-20
JP2020078324A (ja) 2020-05-28
EP3049537A1 (en) 2016-08-03
WO2015042526A1 (en) 2015-03-26
JP2016531550A (ja) 2016-10-13
US20200140929A1 (en) 2020-05-07
US10407739B2 (en) 2019-09-10
EP3049537B1 (en) 2020-03-04
EP3049537A4 (en) 2017-05-10
US20150087545A1 (en) 2015-03-26
CA2925168C (en) 2021-10-26
JP7086122B2 (ja) 2022-06-17

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