CY1109058T1 - Μια μεθοδος για απομονωση ενος πολυνουκλεοτιδιου ενδιαφεροντος απο το γονιδιωμα ενος μυκοβακτηριδιου χρησιμοποιωντας μια bac-βασισμενη dna βιβλιοθηκη. εφαρμογη στην ανιχνευση μυκοβακτηριδιων - Google Patents
Μια μεθοδος για απομονωση ενος πολυνουκλεοτιδιου ενδιαφεροντος απο το γονιδιωμα ενος μυκοβακτηριδιου χρησιμοποιωντας μια bac-βασισμενη dna βιβλιοθηκη. εφαρμογη στην ανιχνευση μυκοβακτηριδιωνInfo
- Publication number
- CY1109058T1 CY1109058T1 CY20091100505T CY091100505T CY1109058T1 CY 1109058 T1 CY1109058 T1 CY 1109058T1 CY 20091100505 T CY20091100505 T CY 20091100505T CY 091100505 T CY091100505 T CY 091100505T CY 1109058 T1 CY1109058 T1 CY 1109058T1
- Authority
- CY
- Cyprus
- Prior art keywords
- bac
- mycobacterium
- isolating
- mycobacteries
- genealm
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/35—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Mycobacteriaceae (F)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1072—Differential gene expression library synthesis, e.g. subtracted libraries, differential screening
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Η παρούσα εφεύρεση εστιάζεται σε μια μέθοδο για απομόνωση ενός πολυνουκλεοτιδίου ενδιαφέροντος το οποίο υπάρχει ή εκφράζεται σε ένα γονιδίωμα ενός πρώτου στελέχους μυκοβακτηριδίου και το οποίο δεν υπάρχει ή είναι αλλαγμένο σε ένα γονιδίωμα ενός δεύτερου στελέχους μυκοβακτηριδίου το οποίο είναι διαφορετικό από το πρώτο στέλεχος μυκοβακτηριδίου χρησιμοποιώντας έναν βακτηριδιακό τεχνητό χρωμοσωμικό (BAC) φορέα. Η εφεύρεση περαιτέρω σχετίζεται με ένα πολυνουκλεοτίδιο απομονωμένο με αυτή τη μέθοδο και ανασυνδυασμένο BAC φορέα ο οποίος χρησιμοποιείται σε αυτή τη μέθοδο. Επιπλέον η παρούσα εφεύρεση περιλαμβάνει μέθοδο και κυτίο για ανίχνευση της παρουσίας μυκοβακτηριδίων σε ένα βιολογικό δείγμα.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/060,756 US6183957B1 (en) | 1998-04-16 | 1998-04-16 | Method for isolating a polynucleotide of interest from the genome of a mycobacterium using a BAC-based DNA library application to the detection of mycobacteria |
EP99913534A EP1071797B1 (en) | 1998-04-16 | 1999-04-16 | A method for isolating a polynucleotide of interest from the genome of a mycobacterium using a bac-based dna library. application to the detection of mycobacteria |
Publications (1)
Publication Number | Publication Date |
---|---|
CY1109058T1 true CY1109058T1 (el) | 2014-07-02 |
Family
ID=22031547
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CY20091100505T CY1109058T1 (el) | 1998-04-16 | 2009-05-11 | Μια μεθοδος για απομονωση ενος πολυνουκλεοτιδιου ενδιαφεροντος απο το γονιδιωμα ενος μυκοβακτηριδιου χρησιμοποιωντας μια bac-βασισμενη dna βιβλιοθηκη. εφαρμογη στην ανιχνευση μυκοβακτηριδιων |
Country Status (10)
Country | Link |
---|---|
US (7) | US6183957B1 (el) |
EP (2) | EP2058325A3 (el) |
AT (1) | ATE423212T1 (el) |
AU (1) | AU3164099A (el) |
CY (1) | CY1109058T1 (el) |
DE (1) | DE69940427D1 (el) |
DK (1) | DK1071797T3 (el) |
ES (1) | ES2322521T3 (el) |
PT (1) | PT1071797E (el) |
WO (1) | WO1999054487A2 (el) |
Families Citing this family (26)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6183957B1 (en) * | 1998-04-16 | 2001-02-06 | Institut Pasteur | Method for isolating a polynucleotide of interest from the genome of a mycobacterium using a BAC-based DNA library application to the detection of mycobacteria |
FR2791067B1 (fr) | 1999-03-16 | 2003-01-31 | Pasteur Institut | Sequence deletees chez m.bovis bcg/m.ou m.tuberculosis, procede de detection des mycobacteries utilisant ces sequences et vaccins |
US7052844B2 (en) * | 1999-12-21 | 2006-05-30 | Ingeneus, Inc. | Purification of DS-DNA using heteropolymeric capture probes and a triplex, quadruplex or homologous duplex binding mechanism |
WO2001077335A2 (fr) * | 2000-04-11 | 2001-10-18 | Institut Pasteur | Genome de listeria monocytogenes, polypeptides et utilisations |
US20020164634A1 (en) * | 2000-08-26 | 2002-11-07 | Perlegen Sciences, Inc. | Methods for reducing complexity of nucleic acid samples |
US20020137043A1 (en) * | 2000-08-26 | 2002-09-26 | Nila Patil | Method for reducing complexity of nucleic acid samples |
US7538206B2 (en) | 2001-02-22 | 2009-05-26 | Institut Pasteur | Comparative mycobacterial geneomics as a tool for identifying targets for the diagnosis, prophylaxis or treatment of mycobacterioses |
US7393539B2 (en) | 2001-06-22 | 2008-07-01 | Health Protection Agency | Mycobacterial antigens expressed under low oxygen tension |
CA2453173C (en) * | 2001-07-04 | 2013-12-10 | Health Protection Agency | Mycobacterial antigens expressed during latency |
WO2003018053A1 (en) * | 2001-08-22 | 2003-03-06 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Vaccine against mycobacterial-induced diseases |
US7439346B2 (en) * | 2001-10-12 | 2008-10-21 | Perkinelmer Las Inc. | Nucleic acids arrays and methods of use therefor |
GB0125535D0 (en) * | 2001-10-24 | 2001-12-12 | Microbiological Res Authority | Mycobacterial genes down-regulated during latency |
US20040023237A1 (en) * | 2001-11-26 | 2004-02-05 | Perelegen Sciences Inc. | Methods for genomic analysis |
US20060183132A1 (en) * | 2005-02-14 | 2006-08-17 | Perlegen Sciences, Inc. | Selection probe amplification |
US20090124514A1 (en) * | 2003-02-26 | 2009-05-14 | Perlegen Sciences, Inc. | Selection probe amplification |
US20050100911A1 (en) * | 2003-08-06 | 2005-05-12 | Perlegen Sciences, Inc. | Methods for enriching populations of nucleic acid samples |
ITRM20030411A1 (it) * | 2003-08-29 | 2005-02-28 | Consiglio Nazionale Ricerche | Uso di sequenze geniche specifiche di mycobacterium tubercolosis ecorrispondenti proteine per la diagnosi e la prevenzione di infezione tubercolare. |
CA2544178A1 (en) * | 2003-10-30 | 2005-05-19 | Tufts-New England Medical Center | Prenatal diagnosis using cell-free fetal dna in amniotic fluid |
ES2679282T3 (es) | 2004-10-22 | 2018-08-23 | Revivicor Inc. | Porcinos transgénicos que carecen de cadena ligera de inmunoglobulina endógena |
ATE530569T1 (de) * | 2005-07-01 | 2011-11-15 | Forsyth Dental In Ry For Children Fa | Assays zum nachweis von tuberkulose- antigenen, und impfstoffe |
CA2638904A1 (en) * | 2006-01-20 | 2007-07-26 | University Of South Florida | Methods for profiling transcriptosomes |
RU2341288C1 (ru) * | 2007-05-10 | 2008-12-20 | Общество С Ограниченной Ответственностью "Биотэк" | СРЕДСТВО, ОБЛАДАЮЩЕЕ СВОЙСТВОМ ФОРМИРОВАТЬ КЛЕТОЧНЫЙ ИММУНИТЕТ ПРОТИВ MYCOBACTERIUM TUBERCULOSIS H37 Rv, СПОСОБ ПОЛУЧЕНИЯ ЕГО (ВАРИАНТЫ), РЕКОМБИНАНТНЫЙ ШТАММ И СРЕДСТВО ДЛЯ ДИАГНОСТИКИ ТУБЕРКУЛЕЗА |
ES2548377T3 (es) | 2008-10-27 | 2015-10-16 | Revivicor, Inc. | Ungulados inmunodeprimidos |
CN102656279A (zh) * | 2009-12-17 | 2012-09-05 | 凯津公司 | 基于全基因组测序的限制性酶 |
US8865404B2 (en) * | 2010-11-05 | 2014-10-21 | President And Fellows Of Harvard College | Methods for sequencing nucleic acid molecules |
WO2015083056A2 (en) * | 2013-12-03 | 2015-06-11 | Kusuma School Of Biological Sciences | Genetic markers for diagnosis of tuberculosis caused by mycobacterium tuberculosis |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2422956A1 (fr) | 1978-04-13 | 1979-11-09 | Pasteur Institut | Procede de detection et de caracterisation d'un acide nucleique ou d'une sequence de celui-ci, et reactif enzymatique pour la mise en oeuvre de ce procede |
FR2518755B1 (fr) | 1981-12-23 | 1986-04-11 | Pasteur Institut | Sonde contenant un acide nucleique modifie et reconnaissable par des anticorps specifiques et utilisation de cette sonde pour detecter et caracteriser une sequence d'adn homologue |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4868105A (en) | 1985-12-11 | 1989-09-19 | Chiron Corporation | Solution phase nucleic acid sandwich assay |
US5202231A (en) | 1987-04-01 | 1993-04-13 | Drmanac Radoje T | Method of sequencing of genomes by hybridization of oligonucleotide probes |
US5776693A (en) * | 1990-06-08 | 1998-07-07 | Institut Pasteur | Specific detection of the mycobacterium tuberculosis |
JP3434818B2 (ja) | 1991-07-31 | 2003-08-11 | ヴァイシス・インコーポレーテッド | 赤痢菌同定用核酸プローブ |
US5474796A (en) * | 1991-09-04 | 1995-12-12 | Protogene Laboratories, Inc. | Method and apparatus for conducting an array of chemical reactions on a support surface |
ATE230440T1 (de) | 1992-03-04 | 2003-01-15 | Genencor Int | Herstellung von -i(aspergillus niger katalase-r) |
JP3645903B2 (ja) | 1992-03-04 | 2005-05-11 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | 比較ゲノムハイブリダイゼーション(cgh) |
US6641814B1 (en) * | 1997-04-02 | 2003-11-04 | Statens Serum Institut | Nucleic acids fragments and polypeptide fragments derived from M. tuberculosis |
NL192999C (nl) | 1994-11-17 | 1998-07-03 | Lederverwerkende Ind De Buffel | Koppeling. |
MX9801687A (es) * | 1995-09-01 | 1998-11-29 | Corixa Corp | Compuestos y metodos para el diagnostico de tuberculosis. |
GB9526178D0 (en) | 1995-12-21 | 1996-02-21 | Taylor John Hermon | Novel polynuceotides and polypeptides in pathogenic mycobacteria and their use as diagnostics,vaccines and targets for chemotherapy |
CA2252542A1 (en) * | 1996-04-29 | 1997-11-06 | Gesellschaft Fur Biotechnologische Forschung Mbh (Gbf) | Dna, rna and a protein useful for detection of a mycobacterial infection |
US6183957B1 (en) * | 1998-04-16 | 2001-02-06 | Institut Pasteur | Method for isolating a polynucleotide of interest from the genome of a mycobacterium using a BAC-based DNA library application to the detection of mycobacteria |
-
1998
- 1998-04-16 US US09/060,756 patent/US6183957B1/en not_active Expired - Lifetime
-
1999
- 1999-04-16 ES ES99913534T patent/ES2322521T3/es not_active Expired - Lifetime
- 1999-04-16 PT PT99913534T patent/PT1071797E/pt unknown
- 1999-04-16 EP EP09152980A patent/EP2058325A3/en not_active Withdrawn
- 1999-04-16 US US09/673,476 patent/US7112663B1/en not_active Expired - Fee Related
- 1999-04-16 WO PCT/IB1999/000740 patent/WO1999054487A2/en active Application Filing
- 1999-04-16 AT AT99913534T patent/ATE423212T1/de active
- 1999-04-16 EP EP99913534A patent/EP1071797B1/en not_active Expired - Lifetime
- 1999-04-16 DK DK99913534T patent/DK1071797T3/da active
- 1999-04-16 DE DE69940427T patent/DE69940427D1/de not_active Expired - Lifetime
- 1999-04-16 AU AU31640/99A patent/AU3164099A/en not_active Abandoned
-
2000
- 2000-09-26 US US09/670,314 patent/US6492506B1/en not_active Expired - Lifetime
-
2002
- 2002-09-30 US US10/259,678 patent/US6991904B2/en not_active Expired - Lifetime
-
2004
- 2004-03-18 US US10/802,796 patent/US7842781B2/en not_active Expired - Fee Related
-
2005
- 2005-09-08 US US11/221,284 patent/US20060063182A1/en not_active Abandoned
-
2006
- 2006-01-30 US US11/341,914 patent/US8455187B2/en not_active Expired - Fee Related
-
2009
- 2009-05-11 CY CY20091100505T patent/CY1109058T1/el unknown
Also Published As
Publication number | Publication date |
---|---|
US7842781B2 (en) | 2010-11-30 |
US8455187B2 (en) | 2013-06-04 |
AU3164099A (en) | 1999-11-08 |
ES2322521T3 (es) | 2009-06-22 |
EP1071797A2 (en) | 2001-01-31 |
EP1071797B1 (en) | 2009-02-18 |
US20030198974A1 (en) | 2003-10-23 |
US20060063182A1 (en) | 2006-03-23 |
WO1999054487A2 (en) | 1999-10-28 |
DK1071797T3 (da) | 2009-06-02 |
US20050250104A1 (en) | 2005-11-10 |
DE69940427D1 (de) | 2009-04-02 |
US7112663B1 (en) | 2006-09-26 |
ATE423212T1 (de) | 2009-03-15 |
WO1999054487A3 (en) | 1999-12-09 |
EP2058325A3 (en) | 2009-10-28 |
US20070092884A1 (en) | 2007-04-26 |
PT1071797E (pt) | 2009-05-20 |
EP2058325A2 (en) | 2009-05-13 |
US6492506B1 (en) | 2002-12-10 |
US6183957B1 (en) | 2001-02-06 |
US6991904B2 (en) | 2006-01-31 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CY1109058T1 (el) | Μια μεθοδος για απομονωση ενος πολυνουκλεοτιδιου ενδιαφεροντος απο το γονιδιωμα ενος μυκοβακτηριδιου χρησιμοποιωντας μια bac-βασισμενη dna βιβλιοθηκη. εφαρμογη στην ανιχνευση μυκοβακτηριδιων | |
EP1961826A3 (en) | A method for direct nucleic acid sequencing | |
ATE368125T1 (de) | Verfahren zur erkennung von einzel-nukleotid- polymorphismen | |
ATE147794T1 (de) | Chemisches verfahren zur analyse von dns- sequenzen | |
EP0188450A4 (en) | METHOD FOR DETECTING PATHOGENS OR GENETIC UNITS UNKNOWN IN VITRO AND DETERMINING. | |
ATE373726T1 (de) | Methode zur detektion eines dna zielmoleküls | |
WO2000077260A8 (en) | Genomic profiling: a rapid method for testing a complex biological sample for the presence of many types of organisms | |
AUPQ909000A0 (en) | A method of detecting microorganisms | |
ATE222606T1 (de) | Multiplex-pcr zum nachweis von infektionen verursacht durch mycobacterium avium komplex | |
CA2203545A1 (en) | Amplification and detection of mycobacterium avium complex species | |
AU2003289927A1 (en) | Multiplex assay detection of pathogenic organisms | |
AU4663997A (en) | Detection and identification of non-polio enteroviruses | |
WO1995033854B1 (en) | Use of a specific marker for detection of salmonella with pcr | |
EP2116617A3 (en) | Probes, probe sets, methods and kits pertaining to the detection, identification and/or enumeration of bacteria | |
CA2324298A1 (en) | Method for typing of hla alleles | |
WO2001014592A3 (en) | Identifying organisms by detecting intronic nucleic acid or encoded proteins | |
EP1266970A3 (en) | Detecting nucleic acid deletion sequences | |
ATE90971T1 (de) | Methode zur analyse einer spezifischen sequenz von dns oder rns, reagenzien und kits dafuer. | |
EP0739987A3 (en) | Oligonucleotides for detecting Salmonella species and detection process using the same | |
DE59107264D1 (de) | Verfahren zur Detektion und quantitativen Bestimmung von Nitrosomonas-Stämmen in Abwässern oder Boden | |
SE9500341D0 (sv) | Method for detecting DNA sequence variations | |
WO1999000517A3 (en) | Biosensor materials and methods | |
NO982039L (no) | FremgangsmÕte og sett for kvantifisering og pÕvisning av mikroorganismer | |
DE69610933D1 (de) | Verfahren und reagenzien zur typisierung von hla-genen der klasse i | |
ATE513061T1 (de) | Diagnostisches verfahren und kit zur bestimmung des kell-blutgruppengenotyps |