CY1109058T1 - Μια μεθοδος για απομονωση ενος πολυνουκλεοτιδιου ενδιαφεροντος απο το γονιδιωμα ενος μυκοβακτηριδιου χρησιμοποιωντας μια bac-βασισμενη dna βιβλιοθηκη. εφαρμογη στην ανιχνευση μυκοβακτηριδιων - Google Patents

Μια μεθοδος για απομονωση ενος πολυνουκλεοτιδιου ενδιαφεροντος απο το γονιδιωμα ενος μυκοβακτηριδιου χρησιμοποιωντας μια bac-βασισμενη dna βιβλιοθηκη. εφαρμογη στην ανιχνευση μυκοβακτηριδιων

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Publication number
CY1109058T1
CY1109058T1 CY20091100505T CY091100505T CY1109058T1 CY 1109058 T1 CY1109058 T1 CY 1109058T1 CY 20091100505 T CY20091100505 T CY 20091100505T CY 091100505 T CY091100505 T CY 091100505T CY 1109058 T1 CY1109058 T1 CY 1109058T1
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CY
Cyprus
Prior art keywords
bac
mycobacterium
isolating
mycobacteries
genealm
Prior art date
Application number
CY20091100505T
Other languages
English (en)
Inventor
Stewart Cole
Roland Buchrieser-Brosch
Stephen Gordon
Alain Billault
Original Assignee
Institut Pasteur
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut Pasteur filed Critical Institut Pasteur
Publication of CY1109058T1 publication Critical patent/CY1109058T1/el

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/35—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Mycobacteriaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1072—Differential gene expression library synthesis, e.g. subtracted libraries, differential screening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Η παρούσα εφεύρεση εστιάζεται σε μια μέθοδο για απομόνωση ενός πολυνουκλεοτιδίου ενδιαφέροντος το οποίο υπάρχει ή εκφράζεται σε ένα γονιδίωμα ενός πρώτου στελέχους μυκοβακτηριδίου και το οποίο δεν υπάρχει ή είναι αλλαγμένο σε ένα γονιδίωμα ενός δεύτερου στελέχους μυκοβακτηριδίου το οποίο είναι διαφορετικό από το πρώτο στέλεχος μυκοβακτηριδίου χρησιμοποιώντας έναν βακτηριδιακό τεχνητό χρωμοσωμικό (BAC) φορέα. Η εφεύρεση περαιτέρω σχετίζεται με ένα πολυνουκλεοτίδιο απομονωμένο με αυτή τη μέθοδο και ανασυνδυασμένο BAC φορέα ο οποίος χρησιμοποιείται σε αυτή τη μέθοδο. Επιπλέον η παρούσα εφεύρεση περιλαμβάνει μέθοδο και κυτίο για ανίχνευση της παρουσίας μυκοβακτηριδίων σε ένα βιολογικό δείγμα.
CY20091100505T 1998-04-16 2009-05-11 Μια μεθοδος για απομονωση ενος πολυνουκλεοτιδιου ενδιαφεροντος απο το γονιδιωμα ενος μυκοβακτηριδιου χρησιμοποιωντας μια bac-βασισμενη dna βιβλιοθηκη. εφαρμογη στην ανιχνευση μυκοβακτηριδιων CY1109058T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/060,756 US6183957B1 (en) 1998-04-16 1998-04-16 Method for isolating a polynucleotide of interest from the genome of a mycobacterium using a BAC-based DNA library application to the detection of mycobacteria
EP99913534A EP1071797B1 (en) 1998-04-16 1999-04-16 A method for isolating a polynucleotide of interest from the genome of a mycobacterium using a bac-based dna library. application to the detection of mycobacteria

Publications (1)

Publication Number Publication Date
CY1109058T1 true CY1109058T1 (el) 2014-07-02

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CY20091100505T CY1109058T1 (el) 1998-04-16 2009-05-11 Μια μεθοδος για απομονωση ενος πολυνουκλεοτιδιου ενδιαφεροντος απο το γονιδιωμα ενος μυκοβακτηριδιου χρησιμοποιωντας μια bac-βασισμενη dna βιβλιοθηκη. εφαρμογη στην ανιχνευση μυκοβακτηριδιων

Country Status (10)

Country Link
US (7) US6183957B1 (el)
EP (2) EP1071797B1 (el)
AT (1) ATE423212T1 (el)
AU (1) AU3164099A (el)
CY (1) CY1109058T1 (el)
DE (1) DE69940427D1 (el)
DK (1) DK1071797T3 (el)
ES (1) ES2322521T3 (el)
PT (1) PT1071797E (el)
WO (1) WO1999054487A2 (el)

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FR2791067B1 (fr) 1999-03-16 2003-01-31 Pasteur Institut Sequence deletees chez m.bovis bcg/m.ou m.tuberculosis, procede de detection des mycobacteries utilisant ces sequences et vaccins
US7052844B2 (en) * 1999-12-21 2006-05-30 Ingeneus, Inc. Purification of DS-DNA using heteropolymeric capture probes and a triplex, quadruplex or homologous duplex binding mechanism
EP1278853A2 (en) * 2000-04-11 2003-01-29 Institut Pasteur Listeria monocytogenes genome, polypeptides and uses
US20020164634A1 (en) * 2000-08-26 2002-11-07 Perlegen Sciences, Inc. Methods for reducing complexity of nucleic acid samples
US20020137043A1 (en) * 2000-08-26 2002-09-26 Nila Patil Method for reducing complexity of nucleic acid samples
EP1401866A2 (en) 2001-02-22 2004-03-31 Institut Pasteur Comparative mycobacterial genomics as a tool for identifying targets for the diagnosis, prophylaxis or treatment of mycobacterioses
JP2005504523A (ja) 2001-06-22 2005-02-17 ヘルス プロテクション エージェンシー 低酸素圧下で発現するマイコバクテリア抗原
AU2002314358C1 (en) 2001-07-04 2009-03-26 The Secretary Of State For Health Mycobacterial antigens expressed during latency
WO2003018053A1 (en) * 2001-08-22 2003-03-06 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Vaccine against mycobacterial-induced diseases
US7439346B2 (en) * 2001-10-12 2008-10-21 Perkinelmer Las Inc. Nucleic acids arrays and methods of use therefor
GB0125535D0 (en) * 2001-10-24 2001-12-12 Microbiological Res Authority Mycobacterial genes down-regulated during latency
US20040023237A1 (en) * 2001-11-26 2004-02-05 Perelegen Sciences Inc. Methods for genomic analysis
US20060183132A1 (en) * 2005-02-14 2006-08-17 Perlegen Sciences, Inc. Selection probe amplification
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Also Published As

Publication number Publication date
AU3164099A (en) 1999-11-08
ES2322521T3 (es) 2009-06-22
EP2058325A3 (en) 2009-10-28
US6492506B1 (en) 2002-12-10
US7842781B2 (en) 2010-11-30
US8455187B2 (en) 2013-06-04
DK1071797T3 (da) 2009-06-02
EP1071797A2 (en) 2001-01-31
PT1071797E (pt) 2009-05-20
US6183957B1 (en) 2001-02-06
US20050250104A1 (en) 2005-11-10
US20070092884A1 (en) 2007-04-26
WO1999054487A3 (en) 1999-12-09
US20060063182A1 (en) 2006-03-23
US6991904B2 (en) 2006-01-31
ATE423212T1 (de) 2009-03-15
EP1071797B1 (en) 2009-02-18
DE69940427D1 (de) 2009-04-02
EP2058325A2 (en) 2009-05-13
WO1999054487A2 (en) 1999-10-28
US20030198974A1 (en) 2003-10-23
US7112663B1 (en) 2006-09-26

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