PT1071797E - Método de isolamento de um polinucleótido de interesse a partir do genoma de uma micobactéria, utilizando uma biblioteca de adn à base de bac. aplicação à detecção de micobactérias - Google Patents

Método de isolamento de um polinucleótido de interesse a partir do genoma de uma micobactéria, utilizando uma biblioteca de adn à base de bac. aplicação à detecção de micobactérias Download PDF

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Publication number
PT1071797E
PT1071797E PT99913534T PT99913534T PT1071797E PT 1071797 E PT1071797 E PT 1071797E PT 99913534 T PT99913534 T PT 99913534T PT 99913534 T PT99913534 T PT 99913534T PT 1071797 E PT1071797 E PT 1071797E
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Prior art keywords
genome
polynucleotide
bac
mycobacteria
mycobacterium
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Application number
PT99913534T
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English (en)
Inventor
Stewart Cole
Roland Buchrieser-Brosch
Stephen Gordon
Alain Billault
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Pasteur Institut
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Publication date
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Publication of PT1071797E publication Critical patent/PT1071797E/pt

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/35Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Mycobacteriaceae (F)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1072Differential gene expression library synthesis, e.g. subtracted libraries, differential screening
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809Methods for determination or identification of nucleic acids involving differential detection

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
PT99913534T 1998-04-16 1999-04-16 Método de isolamento de um polinucleótido de interesse a partir do genoma de uma micobactéria, utilizando uma biblioteca de adn à base de bac. aplicação à detecção de micobactérias PT1071797E (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/060,756 US6183957B1 (en) 1998-04-16 1998-04-16 Method for isolating a polynucleotide of interest from the genome of a mycobacterium using a BAC-based DNA library application to the detection of mycobacteria

Publications (1)

Publication Number Publication Date
PT1071797E true PT1071797E (pt) 2009-05-20

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Family Applications (1)

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PT99913534T PT1071797E (pt) 1998-04-16 1999-04-16 Método de isolamento de um polinucleótido de interesse a partir do genoma de uma micobactéria, utilizando uma biblioteca de adn à base de bac. aplicação à detecção de micobactérias

Country Status (10)

Country Link
US (7) US6183957B1 (pt)
EP (2) EP2058325A3 (pt)
AT (1) ATE423212T1 (pt)
AU (1) AU3164099A (pt)
CY (1) CY1109058T1 (pt)
DE (1) DE69940427D1 (pt)
DK (1) DK1071797T3 (pt)
ES (1) ES2322521T3 (pt)
PT (1) PT1071797E (pt)
WO (1) WO1999054487A2 (pt)

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US6183957B1 (en) * 1998-04-16 2001-02-06 Institut Pasteur Method for isolating a polynucleotide of interest from the genome of a mycobacterium using a BAC-based DNA library application to the detection of mycobacteria
FR2791067B1 (fr) 1999-03-16 2003-01-31 Pasteur Institut Sequence deletees chez m.bovis bcg/m.ou m.tuberculosis, procede de detection des mycobacteries utilisant ces sequences et vaccins
US7052844B2 (en) * 1999-12-21 2006-05-30 Ingeneus, Inc. Purification of DS-DNA using heteropolymeric capture probes and a triplex, quadruplex or homologous duplex binding mechanism
EP1278853A2 (en) * 2000-04-11 2003-01-29 Institut Pasteur Listeria monocytogenes genome, polypeptides and uses
US20020137043A1 (en) * 2000-08-26 2002-09-26 Nila Patil Method for reducing complexity of nucleic acid samples
US20020164634A1 (en) * 2000-08-26 2002-11-07 Perlegen Sciences, Inc. Methods for reducing complexity of nucleic acid samples
AU2002302919A1 (en) * 2001-02-22 2002-10-03 Institut Pasteur Comparative mycobacterial geneomics as a tool for identifying targets for the diagnosis, prophylaxis or treatment of mycobacterioses
JP2005504523A (ja) * 2001-06-22 2005-02-17 ヘルス プロテクション エージェンシー 低酸素圧下で発現するマイコバクテリア抗原
US7393540B2 (en) 2001-07-04 2008-07-01 Health Protection Agency Mycobacterial antigens expressed during latency
WO2003018053A1 (en) * 2001-08-22 2003-03-06 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Vaccine against mycobacterial-induced diseases
US7439346B2 (en) * 2001-10-12 2008-10-21 Perkinelmer Las Inc. Nucleic acids arrays and methods of use therefor
GB0125535D0 (en) * 2001-10-24 2001-12-12 Microbiological Res Authority Mycobacterial genes down-regulated during latency
US20040023237A1 (en) * 2001-11-26 2004-02-05 Perelegen Sciences Inc. Methods for genomic analysis
US20090124514A1 (en) * 2003-02-26 2009-05-14 Perlegen Sciences, Inc. Selection probe amplification
US20060183132A1 (en) * 2005-02-14 2006-08-17 Perlegen Sciences, Inc. Selection probe amplification
US20050100911A1 (en) * 2003-08-06 2005-05-12 Perlegen Sciences, Inc. Methods for enriching populations of nucleic acid samples
ITRM20030411A1 (it) * 2003-08-29 2005-02-28 Consiglio Nazionale Ricerche Uso di sequenze geniche specifiche di mycobacterium tubercolosis ecorrispondenti proteine per la diagnosi e la prevenzione di infezione tubercolare.
US20070212689A1 (en) * 2003-10-30 2007-09-13 Bianchi Diana W Prenatal Diagnosis Using Cell-Free Fetal DNA in Amniotic Fluid
US20060130157A1 (en) 2004-10-22 2006-06-15 Kevin Wells Ungulates with genetically modified immune systems
CN101365714A (zh) * 2005-07-01 2009-02-11 福赛特儿童口腔医院 结核抗原的检测分析及疫苗
US20070172871A1 (en) * 2006-01-20 2007-07-26 Dishaw Larry J Methods for profiling transcriptosomes
RU2341288C1 (ru) * 2007-05-10 2008-12-20 Общество С Ограниченной Ответственностью "Биотэк" СРЕДСТВО, ОБЛАДАЮЩЕЕ СВОЙСТВОМ ФОРМИРОВАТЬ КЛЕТОЧНЫЙ ИММУНИТЕТ ПРОТИВ MYCOBACTERIUM TUBERCULOSIS H37 Rv, СПОСОБ ПОЛУЧЕНИЯ ЕГО (ВАРИАНТЫ), РЕКОМБИНАНТНЫЙ ШТАММ И СРЕДСТВО ДЛЯ ДИАГНОСТИКИ ТУБЕРКУЛЕЗА
ES2548377T3 (es) 2008-10-27 2015-10-16 Revivicor, Inc. Ungulados inmunodeprimidos
AU2010330936B2 (en) * 2009-12-17 2014-05-22 Keygene N.V. Restriction enzyme based whole genome sequencing
US8865404B2 (en) * 2010-11-05 2014-10-21 President And Fellows Of Harvard College Methods for sequencing nucleic acid molecules
US10597734B2 (en) * 2013-12-03 2020-03-24 European Molecular Biology Laboratory Genetic markers for diagnosis of tuberculosis caused by Mycobacterium tuberculosis

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FR2422956A1 (fr) 1978-04-13 1979-11-09 Pasteur Institut Procede de detection et de caracterisation d'un acide nucleique ou d'une sequence de celui-ci, et reactif enzymatique pour la mise en oeuvre de ce procede
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US6183957B1 (en) * 1998-04-16 2001-02-06 Institut Pasteur Method for isolating a polynucleotide of interest from the genome of a mycobacterium using a BAC-based DNA library application to the detection of mycobacteria

Also Published As

Publication number Publication date
DE69940427D1 (de) 2009-04-02
EP2058325A2 (en) 2009-05-13
AU3164099A (en) 1999-11-08
WO1999054487A3 (en) 1999-12-09
CY1109058T1 (el) 2014-07-02
EP1071797A2 (en) 2001-01-31
US20030198974A1 (en) 2003-10-23
US6183957B1 (en) 2001-02-06
US6492506B1 (en) 2002-12-10
US7112663B1 (en) 2006-09-26
EP2058325A3 (en) 2009-10-28
US6991904B2 (en) 2006-01-31
US7842781B2 (en) 2010-11-30
EP1071797B1 (en) 2009-02-18
US20060063182A1 (en) 2006-03-23
US20070092884A1 (en) 2007-04-26
WO1999054487A2 (en) 1999-10-28
ATE423212T1 (de) 2009-03-15
US8455187B2 (en) 2013-06-04
DK1071797T3 (da) 2009-06-02
ES2322521T3 (es) 2009-06-22
US20050250104A1 (en) 2005-11-10

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