US20060063182A1 - Method for isolating a polynucleotide of interest from the genome of a mycobacterium using a BAC-based DNA library: application to the detection of mycobacteria - Google Patents

Method for isolating a polynucleotide of interest from the genome of a mycobacterium using a BAC-based DNA library: application to the detection of mycobacteria Download PDF

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US20060063182A1
US20060063182A1 US11/221,284 US22128405A US2006063182A1 US 20060063182 A1 US20060063182 A1 US 20060063182A1 US 22128405 A US22128405 A US 22128405A US 2006063182 A1 US2006063182 A1 US 2006063182A1
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seq
clone
bac
polynucleotide
dna
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Stewart Cole
Roland Buchrieser-Brosch
Stephen Gordon
Alain Billault
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Institut Pasteur de Lille
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/35—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Mycobacteriaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1072—Differential gene expression library synthesis, e.g. subtracted libraries, differential screening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

Definitions

  • the present invention pertains to a method for isolating a polynucleotide of interest that is present in the genome of a mycobacterium strain and/or is expressed by said mycobacterium strain and that is absent or altered in the genome of a different mycobacterium strain and/or is not expressed in said different mycobacterium strain, said method comprising the use of at least one clone belonging to a genomic DNA library of a given mycobaterium strain, said DNA library being cloned in a bacterial artificial chromosome (BAC).
  • BAC bacterial artificial chromosome
  • the invention concerns also polynucleotides identified by the above method, as well as detection methods for mycobacteria, particularly Mycobacterium tuberculosis , and kits using said polynucleotides as primers or probes. Finally, the invention deals with BAC-based mycobacterium DNA libraries used in the method according to the invention and particularly BAC-based Mycobacterium tuberculosis and Mycobacterium bovis BCG DNA libraries.
  • Cosmid clones (Balasubramanian et al., 1996; Pavelka et al., 1996) have played a crucial role in the M. tuberculosis H37Rv genome sequencing project.
  • problems such as under-representation of certain regions of the chromosome, unstable inserts and the relatively small insert size complicated the production of a comprehensive set of canonical cosmids representing the entire genome.
  • BAC Bacterial Artificial Chromosome
  • the BAC system can also be used for mycobacterial DNA, as 70% of the clones contained inserts in the size of 25 to 104 kb.
  • BAC Bacterial Artificial Chromosome
  • BACs have been widely used for cloning of DNA from various eucaryotic species (Cat et al., 1995; Kim et al., 1996; Misumi et al., 1997; Woo et al., 1994; Zimmer et al., 1997). In contrast, to our knowledge this report describes the first attempt to use the BAC system for cloning bacterial DNA.
  • a central advantage of the BAC cloning system over cosmid vectors used in prior art is that the F-plasmid is present in only one or a maximum of two copies per cell, reducing the potential for recombination between DNA fragments and, more importantly, avoiding the lethal overexpression of cloned bacterial genes.
  • the presence of the BAC as just a single copy means that plasmid DNA has to be extracted from a large volume of culture to obtain sufficient DNA for sequencing and it is described here in the examples a simplified protocol to achieve this.
  • the stability and fidelity of maintenance of the clones in the BAC library represent ideal characteristics for the identification of genomic differences possibly responsible for phenotypic variations in different mycobacterial species.
  • BACs can be allied with conventional hybridization techniques for refined analyses of genomes and transcriptional activity from different mycobacterial species.
  • BAC-based genomic mycobacterial DNA libraries constructed by the inventors have allowed them to design methods and means both useful to identify genomic regions of interest of pathogenic mycobacteria, such as Mycobacterium tuberculosis , that have no counterpart in the corresponding non-pathogenic strains, such as Mycobacterium bovis BCG, and useful to detect the presence of polynucleotides belonging to a specific mycobacterium strain in a biological sample.
  • a biological sample By a biological sample according to the present invention, it is notably intended a biological fluid, such as plasma, blood, urine or saliva, or a tissue, such as a biopsy.
  • a biological fluid such as plasma, blood, urine or saliva
  • a tissue such as a biopsy.
  • a first object of the invention consists of a method for isolating a polynucleotide of interest that is present in the genome of a mycobacterium strain and/or is expressed by said mycobacterium strain and that is absent or altered in the genome of a different mycobacterium strain and/or is not expressed in said different mycobacterium strain, said method comprising the use of at least one clone belonging to a genomic DNA library of a given mycobaterium strain, said DNA library being cloned in a bacterial artificial chromosome (BAC).
  • BAC bacterial artificial chromosome
  • the invention is also directed to a polynucleotide of interest that has been isolated according to the above method and in partoular a polynucleotide containing one or several Open Reading Frames (ORFs), for example ORFs encoding either a polypeptide involved in the pathogenicity of a mycobacterium strain or ORFs encoding Polymorphic Glycine Rich Sequences (PGRS).
  • ORFs Open Reading Frames
  • PGRS Polymorphic Glycine Rich Sequences
  • Such polynucleotides of interest may serve as probes or primers in order to detect the presence of a specific myobacterium strain in a biological sample or to detect the expression of specific genes in a particular mycobacterial strain of interest.
  • the BAC-based genomic mycobacterial DNA libraries generated by the present inventors are also part of the invention, as well as each of the recombinant BAC clones and the DNA insert contained in each of said recombinant BAC clones.
  • the invention also pertains to methods and kits for detecting a specific mycobacterium in a biological sample using either at least one recombinant BAC clone or at least one polynucleotide according to the invention, as well as to methods and kits to detect the expression of one or several specific genes of a given mycobacterial strain present in a biological sample.
  • FIG. 1 PCR-screening for unique BAC clones with specific primers for 2 selected genomic regions of the H37Rv chromosome, using 21 pools representating 2016 BACs (Panel A) and sets of 20 subpools from selected positive pools (Panel B).
  • FIG. 2 Pulsed-field gel electrophoresis gel of DraI-cleaved BAC clones used for estimating the insert sizes of BACs.
  • FIG. 3 Minimal overlapping BAC map of M. tuberculosis H37Rv superimposed on the integrated physical and genetic map established by Philipp et al. (18).
  • Y- and I-numbers show pYUB328 (2) and pYUB412 (16) cosmids which were shotgun sequenced during the H37Rv genome sequencing project.
  • Y-cosmids marked with * were shown in the integrated physical and genetic map (18).
  • Rv numbers show the position of representative BAC clones relative to sequenced Y- and I-clones. Squared Rv numbers show BACs which were shotgun sequenced at the Sanger Centre.
  • FIG. 4 Ethidium bromide stained gel (Panel A) and corresponding Southern blot (Panel B) of EcoRI and PvuII digested Rv58 DNA hybridized with 32 P labeled genomic DNA preparations from M. tuberculosis H37Rv, M. bovis ATCC 19210 and M. bovis BCG Pasteur.
  • FIG. 5 Organisation of the ORFs in the 12.7 kb genomic region present in M. tuberculosis H37Rv but not present in M. bovis ATCC 19210 and M. bovis BCG Pasteur. Arrows show the direction of transcription of the putative genes. Positions of EcoRI and PvuII restriction sites are shown. Vertical dashes represent stop codons.
  • the 11 ORFs correspond to the ORFs MTCY277.28 to MTCY277.38/accession number Z79701-EMBL Nucleotide Sequence Data Library. The junction sequences flanking the polymorphic region are shown.
  • FIG. 6 Variation in the C-terminal part of a PE-PGRS open reading frame in M. tuberculosis strain H37Rv relative to M. bovis BCG strain Pasteur.
  • FIG. 7 Polynucleotide sequence next to the HindIII cloning site in the BAC vector pBeloBAC11 (Kim et al., 1996) used to clone the inserts of the BAC-based myobacterial genomic DNA library according to the invention.
  • the present invention is directed to a method for isolating a polynucleotide of interest that is present in the genome of a mycobacterium strain and/or is expressed by said mycobacterium strain and that is absent or altered in the genome of a different mycobacterium strain and/or is not expressed in said different mycobacterium strain, said method comprising the use of at least one clone belonging to a genomic DNA library of a given mycobaterium strain, said DNA library being cloned in a bacterial artificial chromosome (BAC).
  • BAC bacterial artificial chromosome
  • the inventors have constructed several BAC-based mycobacterial genomic DNA libraries that may be used in order to perform the above described method.
  • an object of the present invention consists in a myobacterial genomic DNA library cloned in such a BAC type vector.
  • BAC-based DNA library of Mycobacterium tuberculosis has been realized. Forty-seven cosmids chosen from the integrated map of the 4.4 Mb circular chromosome (Philipp et al., 1996a) were shotgun-sequenced during the initial phase of the H37Rv genome sequence project. The sequences of these clones were used as landmarks in the construction of a minimally overlapping BAC map. Comparison of the sequence data from the termini of 420 BAC clones allowed us to establish a minimal overlapping BAC map and to fill in the existing gaps between the sequence of cosmids.
  • the Bacterial Artificial Chromosome (BAC) cloning system is capable of stably propagating large, complex DNA inserts in Escherichia coli .
  • a BAC library was constructed in the pBeloBAC11 vector and used for genome mapping, confirmation of sequence assembly, and sequencing.
  • the library contains about 5000 BAC clones, with inserts ranging in size from 25 to 104 kb, representing theoretically a 70 fold coverage of the M. tuberculosis genome (4.4 Mb).
  • a total of 840 sequences from the T7 and SP6 termini of 420 BACs were determined and compared to those of a partial genomic database.
  • BAC clones represent linking clones between sequenced cosmids, allowing fall-coverage of the H37Rv chromosome, and they are now being shotgun-sequenced in the framework of the H37Rv sequencing project. Also, no chimeric, deleted or rearranged BAC clones were detected, which was of major importance for the correct mapping and assembly of the H37Rv sequence.
  • the minimal overlapping set contains 68 unique BAC clones and spans the whole H37Rv chromosome with the exception of a single gap of ⁇ 150 kb.
  • the canonical BAC set was used in a comparative study to reveal chromosomal polymorphisms between M. tuberculosis, M. bovis and M. bovis BCG Pasteur, and a novel 12.7 kb segment present in M. tuberculosis but absent from M. bovis and M. bovis BCG was characterized.
  • This region contains a set of genes whose products show low similarity to proteins involved in polysaccharide biosynthesis.
  • the H37Rv BAC library therefore provides the one skilled in the art with a powerful tool both for the generation and confirmation of sequence data as well as for comparative genomics and a plurality of post-genomic applications.
  • BAC-based Mycobacterium tuberculosis genomic DNA library is part of the present invention and has been deposited in the Collection Nationale de Cultures de Microorganismes (CNCM) on Nov. 19, 1997 under the accession number I-1945.
  • BAC-based DNA library has been constructed with the genomic DNA of Mycobacterium bovis BCG, Pasteur strain, and said DNA library has been deposited in the Collection Nationale de Cultures de Microorganismes (CNCM) on XX XX, 1998 under the accession number I-XXXX.
  • said method makes use of at least one BAC-based DNA library that has beeen constructed from the genomic DNA of Mycobacterium tuberculosis , more specifically of the H37Rv strain and particularly of the DNA library deposited in the accession number I-1945.
  • said method makes use of at least one BAC-based DNA library has beeen constructed from the genomic DNA of Mycobacterium bovis BCG, more specifically of the Pasteur strain and particularly of the DNA library deposited in the accession number I-XXXX.
  • the method according to the invention for isolating a polynucleotide of interest may comprise the following steps:
  • the at least one polynucleotide of step a) may be prepared as follows:
  • the above method of the invention allows the one skilled in the art to perform comparative genomics between different strains or species of mycobacteria cells, for example between pathogenic strains or species and their non pathogenic strains or species counterparts, as it is the illustrative case for the genomic comparison between Mycobacterium tuberculosis and Mycobacterium bovis BCG that is described herein in the examples.
  • Restriction digests of a given clone of a BAC library according to the invention may be blotted to membranes, and then probed with radiolabeled DNA form another strain or another species of mycobacteria, allowing the one skilled in the art to identify, characterize and isolate a polynucleotide of interest that may be involved in important metabolical and/or physiological pathways of the mycobacterium under testing, such as a polynucleotide functionally involved in the pathogenicity of said given mycobacteria for its host organism.
  • Example 6 when restriction digests of a given clone of the BAC library identified by the CNCM accession number I-1945 are blotted to membranes and then probed with radiolabeled total genomic DNA from, for example, Mycobacterium bovis BCG Pasteur, it is observed that restriction fragments that fail to hybridize with the M. bovis BCG Pasteur DNA are absent from its genome, hence identifying polymorphic regions between M. bovis BCG Pasteur and M. tuberculosis H37Rv.
  • a further object of the present invention consists in a polynucleotide of interest that has been isolated according to the method described herein before.
  • Example 6 a polynucleotide of approximately 12.7 kilobases has been isolated that is present in the genome of M. tuberculosis but is absent of the genome of M, bovis BCG.
  • This polynucleotide of interest contains 11 ORFs that may be involved in polysaccharide biosynthesis.
  • two of said ORFs are of particular interest, namely ORF6 (MTCY277.33; Rv1511) that encodes a protein that shares significant homology with bacterial GDP-D-mannose dehydratases, whereas the protein encoded by ORF7 (MTCY277.34; Rv1512) shares significant homology with a nucleotide sugar epimerase.
  • polysaccharide is a major constituent of the mycobacterial cell wall these deleted genes may cause the cell wall of M. bovis BCG to differ from that of M. tuberculosis , a fact that may have important consequences for both the immune response to M. bovis BCG and virulence. Detection of such a polysaccharide is of diagnostic interest and possibly useful in the design of tuberculosis vaccines.
  • the polynucleotide of interest obtained following the method according to the invention may contain at least one ORF, said ORF preferably encoding all or part of a polypeptide involved in an important metabolical and/or physiological pathway of the mycobacteria under testing and more specifically all or part of a polypeptide that is involved in the pathogenicity of the mycobacteria under testing, such as for example Mycobacterium tuberculosis , and more generally mycobacteria belonging to the Mycobacterium tuberculosis complex.
  • the Mycobacterium tuberculosis complex has its usual meaning, i.e. the complex of mycobacteria causing tuberculosis which are Mycobacterium tuberculosis, Mycobacterium bovis, Mycobacterium africanum, Mycobacterium microti and the vaccine strain Mycobacterium bovis BCG.
  • An illustrative polynucleotide of interest according to the present invention comprises all or part of the polynucleotide of approximately 12.7 kilobases that is present in the genome of M. tuberculosis but is absent from the genome of M. bovis BCG disclosed hereinbefore. This polynucleotide is contained in clone Rv58 of the BAC DNA library I-1945.
  • the invention also pertains to a purified polynucleotide comprising the DNA insert contained in a recombinant BAC vector belonging to a BAC-based mycobacterial genomic DNA library, such as for example the I-1945 BAC DNA library.
  • Such a polynucleotide of interest may be used as a probe or a primer useful for specifically detecting a given mycobacterium of interest, such as Mycobacterium tuberculosis or Mycobacterium bovis BCG.
  • the invention then deals with a purified polynucleotide useful as probe or a primer comprising all or part of the nucleotide sequence SEQ ID N o 1: ACCTGCGCTT GCAGAGATCA AATAGGGCGC ATGGGTCAGC 60 ATAGTACAGG TCGTCGCGCA TCTTTGATGC ATCGGAATAA GATGTCAGGC AATTAAAAGA 120 GAAGCCACGG CGACTCGCGG CATTCAGCAT GTCGAGCGTC GCTTCGATGT GAGCACCA 180 TTCCGTGTCC AACGATTTCA GACGAACATT GAATATTCCA CTCGCGACGC TATAGTCCGC 240 CTCCCGATCT ATGCGCCG CGCAGATGAA GTCTGCGTTC GCCCGACCTT CGAAACGTAG 300 TGCGGCCGCG CGCACCATTT CGGGGGAGAC GTCGATGCCG GTGTAATCAG TTTTGAAGCC 360 ACGCGCATCT AGGTAGTCCA GTAGCCCC ATAGCCACA
  • this 12.7 kb deletion should allow a rapid PCR screening of tubercle isolates to identify whether they are bovine or human strains.
  • the primers listed in Table 1 are flanking the deleted region and give a 722 bp amplicon in M. bovis or M. bovis BCG strains, but a fragment of 13,453 bp in M. tuberculosis that is practically impossible to amplify under the same PCR conditions. More importantly, assuming that some of the gene products from this region represent proteins with antigenic properties, it could be possible to develop a test that can reliably distinguish between the immune response induced by vaccination with M. bovis BCG vaccine strains and infection with M. tuberculosis or that the products (e.g. polysaccharides) are specific immunogens.
  • the invention also provides for a purified polynucleotide useful as a probe or as a primer, said polynucleotide being chosen in the following group of polynucleotides:
  • the hybridization step is realized at 65° C. in the presence of 6 ⁇ SSC buffer, 5 ⁇ Denhardt's solution, 0.5% SDS and 100 ⁇ g/ml of salmon sperm DNA.
  • 1 ⁇ SSC corresponds to 0.15 M NaCl and 0.05M sodium citrate
  • 1 ⁇ Denhardt's solution corresponds to 0.02% Ficoll, 0.02% polyvinylpyrrolidone and 0.02% bovine serum albumin.
  • the hybridization step is followed by four washing steps:
  • a first illustrative useful polynucleotide that is included in the polynucleotide of sequence SEQ ID N o 1 is the following polynucleotide of sequence SEQ ID N o 2 that corresponds to the Sp6 endsequence of SEQ ID N o 1: ATACTCAAGC TTGCCGCAAT CGAAACCAAC CTGTTTGTGC 60 CGCAAGAAAT TACGCCGTGG CCCGGCCG ATCAAGAAAC GCCCCGGCGC GCGGCGGTGT 120 CGTCGTATGG CATGACGGGC ACCAATGTGC ACGCCATTGT CGAGCAGGCA CCGGTGCCAG 180 CCCCCGAATC CGGTGCACCA GGCGACACCC CGGCCACACC CGGTATCGAC GGCGCTGC 240 TGTTCGCGCT GTCGGCCAGC TCGCAGGACG CGCTGCGGCA AACCGCCGCGCGCGTGGCCG 289 ATTGGGTCT
  • a second illustrative useful polynucleotide that is included in the polynucleotide of sequence SEQ ID N o 1 is the following polynucleotide of sequence SEQ ID N o 3 that corresponds to the T7 endsequence of SEQ ID N o 1, located on the opposite strand: TTGGCGGGTT GGCCACACAC CCGCCGGTGA CGGCGACGAT 60 GCTGGGCTGG TTGCGGCCCT GCGCCACCGC GGCTTGCATG CTGGTTGGCT GTCTTGGGAC 120 GATCCCGAAA TAGTCCACGC GGATCTGGTG ATTTTGCGGG CTACCCGCGA TTACCCCGCG 180 CGGCTCGACG AGTTTTTGGC CTGGACTACC CGCGTGGCCA ATCTGCTGAA CTCGCGCGCGCG 240 GTGGTGGCCT GGAATGTCCA CGCCGTTCAC CTACGTGACC TTGATGGGAT 278 CCGGGGGT
  • polynucleotide of sequence SEQ ID N o 1 contains 11 ORFs, the respective locations of which, taking into account the orientation of each ORF on the chromosome, on the sequence of the Mycobacterium tuberculosis chromosome, is given hereafter:
  • ORF11 encodes a protein similar to a spore coat polysaccharide biosynthesis.
  • a polynucleotide of interest obtained by the above-disclosed method according to the invention may also contain at least one ORF that encodes all or part of acidic, glycine-rich proteins, belonging to the PE and PPE families, whose genes are often clustered and based on multiple copies of the polymorphic repetitive sequences.
  • the names PE and PPE derive from the fact that the motifs ProGlu (PE, positions 8, 9) and ProProGlu (PPE, positions 7 to 9) are found near the N-terminus in almost all cases.
  • the PE protein family all have a highly conserved N-terminal domain of ⁇ 110 amino acid residues, that is predicted to have a globular structure, followed by a C-terminal segment which varies in size, sequence and repeat copy number.
  • Phylogenetic analysis separated the PE family into several groups, the larger of which is the highly repetitive PGRS class containing 55 members whereas the other groups share very limited sequence similarity in their C-terminal domains.
  • the predicted molecular weights of the PE proteins vary considerably as a few members only contain the ⁇ 110 amino acid N-terminal domain while the majority have C-terminal extensions ranging in size from 100 up to >1400 residues.
  • a striking feature of the PGRS proteins is their exceptional glycine content (up to 50%) due to the presence of multiple tandem repetitions of GlyGlyAla or GlyGlyAsn motifs or variations thereof.
  • the PPE protein family also has a conserved N-terminal domain that comprises ⁇ 180 amino acid residues followed by C-terminal segments that vary considerably in sequence and length. These proteins fall into at least three groups, one of which constitutes the MPTR class characterised by the presence of multiple, tandem copies of the motif AsnXGlyXGlyAsnXGly. The second subgroup contains a characteristic, well-conserved motif around position 350 (GlyXXSerValProXXTrp), whereas the other group contains proteins that are unrelated except for the presence of the common 180-residue PPE domain. C-terminal extensions may range in size from 00 up to 3500 residues.
  • PGRS sub-family the WHO antigen 22T (Abou-Zeid et al., 1991), a 55 kD protein capable of binding fibronectin, is produced during disease and elicits a variable antibody response suggesting either that individuals mount different immune responses or that this PGRS-protein may not be produced in this form by all strains of M. tuberculosis .
  • at least some PE_PGRS coding sequences encode for proteins that are involved in the recognition of M. tuberculosis by the immune system of the infected host. Therefore, differences in the PGRS sequences could represent the principal source of antigenic variation in the otherwise genetically and antigenically homogeneous bacterium.
  • one ORF contained in BAC vector N o Rv418 of the M. tuberculosis BCG I-1945 DNA library carries both base additions and base deletions when compared with the corresponding ORF in the genome of M. bovis BCG that is contained in the BAC vector N o X0175 of the M. bovis BCG I-XXXX DNA libary.
  • the variations observed in the base sequences correspond to variations in the C-terminal part of the aminoacid sequence of the PGRS ORF translation product.
  • an amino acid stretch of 9 residues in length is present in this M. tuberculosis PGRS (ORf reference Rv0746) and is absent from the ORF counterpart of M. bovis BCG, namely the following amino acid sequence: NH 2 -GGAGGAGGSSAGGGGAGGAGGAGGWLLGD-COOH.
  • FIG. 6 shows also that an amino acid stretch of 45 residues in length is absent from this M. tuberculosis PGRS and is present in the ORF counterpart of M. bovis BCG, namely following amino acid sequence: NH 2 -GAGGIGGIGGNANGGAGGNGGTGGQLWGSGGAGVEGGAALSVGDT- COOH.
  • novel and nonobvious PGRS polynucleotide from M. bovis which is homolog to the M. tuberculosis ORF Rv0746, and which is contained in the BAC clone N o X0175 (See Table 4 for SP6 and T7 endsequences of clone n o X0175) of the I-XXXX M. bovis BCG BAC DNA library is part of the present invention, as it represents a starting material in order to define specific probes or primers useful for detection of antigenic variability in mycobacterial strains, possible inhibition of antigen processing as well as to differentiate M. tuberculosis from M. bovis BCG.
  • a further object of the invention consists in a polynucleotide comprising the following sequence SEQ ID N o 4: CCGACCCAGA CACTGACCGG GCGACCGCTG ATCGGCAACG 60 GCACCCCCGG GGCGGTCCGC AGCGGGGCCA CCGGGGCCCC CGGTGGGTGG CTGCTCGGCG 120 ACGGCGGGGC CGGCGGGTCC GGCGCGGCGG GCTCGGGCGC GCCCGGCGGG GCGGGCGGGG 180 CTGCCGGGCT GTGGGGTACC GGCGGGGCCG GCGGGATCGG CGGAGCCAGC ACCGTACTCG 240 GCGGCACCGG CGGGGGAGGC GGGGTCGGTG GGCTGTGGGG CGCCGGTGGG GCCGGCGGGG 300 CCGGTGGAAC CGGCCTTGTT GGTGGCGACG GCGGGGCCGG TGGGGCCGGC GGGACCGGCG 360 GACTGCTGGC CGGGCTGATC GGTGCCGGCG GAGGTCACGG
  • Polynucleotides of interest have been defined by the inventors as useful detection tools in order to differentiate M. tuberculosis from M. bovis BCG. Such polynucleotides are contained in the 45 aminoacid length coding sequence that is present in M. bovis BCG but absent from M. tuberculosis . This polynucleotide has a sequence beginning (5′end) at the nucleotide at position nt 729 of the sequence SEQ ID N o 4 and ending (3′end) at the nucleotide in position nt 863 of the sequence SEQ ID N o 4.
  • part of the present invention is also a polynucleotide which is chosen among the following group of polynucleotides:
  • a polynucleotide comprising at least 8 consecutive nucleotides the following nucleotide sequence SEQ ID N o 5: GGGCATCGGC GGAATCGGCG GTAACGCTAA CGGGGGCGCC 60 GGTGGGAACG GCGGCACCGG CGGTCAGTTA TGGGGTAGCG GCGGCGCCGG CGTCGAAGGC 120 GGCGCAGCCT TAAGCGTCGG CGACACC 127
  • the invention also provides for a BAC-based Mycobacterium tuberculosis strain H37Rv genomic DNA library that has been deposited in the Collection Nationale de Cultures de Microorganismes on Nov. 19, 1997 under the accession number I-1945.
  • a further object of the invention consists in a recombinant BAC vector which is choosen among the group consisting of the recombinant BAC vectors belonging to the BAC-based DNA library I-1945.
  • a recombinant BAC vector of interest may be choosen among the following set or group of BAC vectors contained in the BAC-based DNA library I-1945:
  • the minimal overlapping set of BAC vectors of the BAC-based DNA library I-1945 contains 68 unique BAC clones and practically spans almost the whole H37Rv chromosome with the exception of a single gap of approximately 150 kb.
  • a recombinant BAC vector of interest is choosen among the following set or group of BAC vectors from the BAC-based DNA library I-1945, the location of which vector DNA inserts on the chromosome of M. tuberculosis is shown in FIG. 3 :
  • polynucleotides disclosed in Table 3 may be used as probes in order to select a given clone of the BAC DNA library I-1945 for further use.
  • the invention also provides for a BAC-based Mycobacterium bovis strain Pasteur genomic DNA library that has been deposited in the Collection Nationale de Cultures de Microorganismes on XXXX XX, 1998 under the accession number I-XXXX.
  • a further object of the invention consists in a recombinant BAC vector which is chosen among the group consisting of the recombinant BAC vectors belonging to the BAC-based DNA library I-XXXX.
  • This DNA library contains approximately 1600 clones.
  • the average insert size is estimated to be ⁇ 80 kb.
  • a recombinant BAC vector of interest may be chosen among the following set or group of BAC vectors contained in the BAC-based DNA library I-XXXX:
  • polynucleotides disclosed in Table 4 may be used as probes in order to select a given clone of the BAC DNA library I-XXXX for further use.
  • polynucleotide inserts that are contained in the above described BAC vectors, that are useful as primers or probes.
  • polynucleotides and nucleic acid fragments may be used as primers for use in amplification reactions, or as nucleic probes.
  • PCR is described in the U.S. Pat. No. 4,683,202.
  • the amplified fragments may be identified by an agarose or a polyacrylamide gel electrophoresis, or by a capillary electrophoresis or alternatively by a chromatography technique (gel filtration, hydrophobic chromatography or ion exchange chromatography).
  • the specificity of the amplification may be ensured by a molecular hybridization using, for example, one of the initial primers as nucleic probes.
  • Amplified nucleotide fragments are used as probes in hybridization reactions in order to detect the presence of one polynucleotide according to the present invention or in order to detect mutations in the genome of the given mycobacterium of interest, specifically a mycobacterium belonging to the Mycobacterium tuberculosis complex and more specifically Mycobacterium tuberculosis and Mycobacterium bovis BCG.
  • amplified nucleic fragments are also part of the present invention the amplified nucleic fragments ( amplicons ) defined herein above.
  • probes and amplicons may be radioactively or non-radioactively labeled, using for example enzymes or fluorescent compounds.
  • nucleic acid amplification may also be used and are generally preferred to the PCR technique.
  • the Strand Displacement Amplification (SDA) technique (Walker et al., 1992) is an isothermal amplification technique based on the ability of a restriction enzyme to cleave one of the strands at his recogntion site (which is under a hemiphosphorothioate form) and on the property of a DNA polymerase to initiate the synthesis of a new strand from the 3′OH end generated by the restriction enzyme and on the property of this DNA polymerase to displace the previously synthesized strand being localized downstream.
  • the SDA method comprises two main steps:
  • the doubling time of the target DNA is of 26 seconds and the amplification rate is of 10 10 after an incubation time of 15 min at 60° C.
  • the SDA amplification technique is more easy to perform than PCR (a single thermostated waterbath device is necessary) and is faster thant the other amplification methods.
  • another object of the present invention consists in using the nucleic acid fragments according to the invention (primers) in a method of DNA or RNA amplification according to the SDA technique.
  • primers for performing SDA, two pairs of primers are used: a pair of external primers (B1, B2) consisting of a sequence specific for the target polynucleotide of interest and a pair of internal primers (S1, S2) consisting of a fusion oligonucleotide carrying a site that is recognized by a restriction endonuclease, for example the enzyme BSOBI.
  • polynucleotides of the invention and their above described fragments, especially the primers according to the invention, are useful as technical means for performing different target nucleic acid amplification methods such as:
  • polynucleotides according to the invention are also useful as technical means for performing methods for amplification or modification of a nucleic acid used as a probe, such as:
  • RNA for example a mRNA
  • a reverse transcriptase enzyme will be used before the amplification reaction in order to obtain a cDNA from the RNA contained in the biological sample.
  • the generated cDNA is subsequently used as the nucleic acid target for the primers or the probes used in an amplification process or a detection process according to the present invention.
  • the non-labeled polynucleotides or oligonucleotides of the invention may be directly used as probes. Nevertheless, the polynucleotides or oligonucleotides are generally labeled with a radioactive element ( 32 P, 35 S, 3 H, 125 I) or by a non-isotopic molecule (for example, biotin, acetylaminofluorene, digoxigenin, 5-bromodesoxyuridin, fluorescein) in order to generate probes that are useful for numerous applications.
  • a radioactive element 32 P, 35 S, 3 H, 125 I
  • a non-isotopic molecule for example, biotin, acetylaminofluorene, digoxigenin, 5-bromodesoxyuridin, fluorescein
  • nucleic acid fragments examples include Urdea et al. or Sanchez-Pescador et al., 1988.
  • the hybridization step may be performed in diffrent ways (Matthews et al., 1988).
  • the more general method consists of immobilizing the nucleic acid that has been extracted from the biological sample onto a substrate (nitrocellulose, nylon, polystyrene) and then to incubate, in defined conditions, the target nucleic acid with the probe. Subsequently to the hybridization step, the excess amount of the specific probe is discarded and the hybrid molecules formed are detected by an appropriate method (radioactivity, fluorescence or enzyme activity measurement).
  • the probes according to the present invention may have structural characteristics such that they allow the signal amplification, such structural characteristics being, for example, branched DNA probes as those described by Urdea et al. in 1991 or in the European patent N o EP-0225,807 (Chiron).
  • the latters may be used as capture probes , and are for this purpose immobilized on a substrate in order to capture the target nucleic acid contained in a biological sample.
  • the captured target nucleic acid is subsequently detected with a second probe which recognizes a sequence of the target nucleic acid which is different from the sequence recognized by the capture probe.
  • the oligonucleotide probes according to the present invention may also be used in a detection device comprising a matrix library of probes immobilized on a substrate, the sequence of each probe of a given length being localized in a shift of one or several bases, one from the other, each probe of the matrix library thus being complementary to a distinct sequence of the target nucleic acid.
  • the substrate of the matrix may be a material able to act as an electron donor, the detection of the matrix poisitons in which an hybridization has occurred being subsequently determined by an electronic device.
  • matrix libraries of probes and methods of specific detection of a targer nucleic acid is described in the European patent application N o EP-0713,016 (Affymax technologies) and also in the U.S. Pat. No. 5,202,231 (Drmanac).
  • BAC-based genomic DNA libraries Since almost the whole length of a mycobacterial chromososme is covered by a BAC-based genomic DNA libraries according to the present invention (i.e. 97% of the M. tuberculosis chromosome is covered by the BAC library I-1945), these DNA libraries will play an important role in a plurality of post-genomic applications, such as in mycobacterial gene expression studies where the canonical set of BACs could be used as a matrix for hybridization studies. Probing such matrices with cDNA probes prepared from total mRNA will uncover genetic loci induced or repressed under different physiological conditions (Chuang et al., 1993; Trieselmann et al., 1992). As such, the H37Rv BAC library represents a fundamental resource for present and future genomics investigations.
  • BAC vectors or the polynucleotide inserts contained therein may be directly used as probes, for example when immobilized on a substrate such as described herein before.
  • the BAC vectors or their polynucleotide inserts may be directly asdorbed on a nitrocellulose membrane, at predetermined locations on which one or several polynucleotides to be tested are then put to hybridize therewith.
  • a collection of BAC vectors that spans the whole genome of the mycobacterium under testing will be immobilized, such as, for example, the set of 68 BAC vectors of the I-1945 DNA library that is described elsewhere in the specification and shown in FIG. 3 .
  • the immobilization and hybridization steps may be performed as described in the present Materials and Methods Section.
  • these vectors may be useful to perform a transcriptional activity analysis of mycobacteria growing in different environmental conditions, for example under conditions in which a stress response is expected, as it is the case at an elevated temperature, for example 40° C.
  • Genescreen membranes may be used to immobilize the restriction endonuclease digests (HindIII digests for the BAC DNA library I-1945) of the BAC vectors by tranfer from a gel (Trieselmann et al., 1992).
  • the BAC vectors may be immobilized for dot blot experiments as follows. First, the DNA concentration of each BAC clone is determined by hybridization of blots of clone DNAs and of a BAC vector concentration standard with a BAC vector specific DNA probe. Hybridization is quantified by the Betascope 603 blot analyzer (Betagen Corp.), which colects beta particles directly from the blot with high efficiency. Then, 0.5 ⁇ g of each clone DNA is incubated in 0.25 M NaOH and 10 mM EDTA at 65° C. for 60 min to denature the DNA and degrade residual RNA contaminants.
  • Betascope 603 blot analyzer Betascope 603 blot analyzer
  • each clone DNA is blotted onto a GeneScreen Plus nylon membrane in the alkaline solution. After neutralization, the blots are baked at 85° C. for 2 h under vacuum. Positive and negative controls are added when necessary. In order to perform this procedure, it may be referred to the article of Chuang et al. (1993).
  • cells grown in a suitable volume of culture medium may, for example, be immediately mixed with an equal volume of crushed ice at ⁇ 70° C. and spun at 4° C. in a 50 ml centrifugation tube.
  • the cell pellet is then suspended in 0.6 ml of ice-cold buffer (10 mM KCl, 5 mM MgCl, 10 mM Tris; pH 7.4) and then immediately added to 0.6 ml of hot lysis buffer (0.4 M NaCl, 40 mM EDTA, 1% beta-mercaptoethanol 1% SDS, 20 mM Tris; pH 7.4) containing 100 ⁇ l of water saturated phenol.
  • This mixture is incubated in a boiling water bath for 40 s.
  • the debris are removed by centrifugation.
  • the supernatant is extracted with phenol-chloroform five times, ethanol precipitated, and dried.
  • the dried RNA pellet is dissolved in water before use.
  • labeled total cDNA may be prepared by the following method.
  • the reaction mixture contains 15 ⁇ g of the previously prepared total RNA, 5 ⁇ g of pd(N 6 ) (random hexamers from Pharmacia Inc.), 0.5 mM dATP, 0.5 mM dGTP and 0.5 mM DTTP, 5 ⁇ M dCTP, 100 ⁇ Ci of [ ⁇ - 32 P]dCTP (3,000 Ci/mmol), 50 mM Tris-HCl (pH 8.3), 6 mM MgCl 2 , 40 mM Kcl, 0.5 U of avian myeloblastosis virus reverse transcriptase (Life Science Inc.) in a total volume of 50 ⁇ l.
  • the reaction is allowed to continue overnight at room temperature.
  • EDTA and NaOH are then added to final concentrations of 50 mM and 0.25 M, respectively, and the mixture is incubated at 65° C. for 30 min to degrade the RNA templates.
  • the cDNA is then ready to use after neutralization by adding Hcl and Tris buffer.
  • the hybridization step may be performed as described by Chuang et al. (1993) and briefly disclosed hereinafter.
  • the DNA dot blot is hybridized to 32 P-labeled total cDNA in a solution containing 0.1% polyvinylpyrrolidone, 0.1% Ficoll, 0.1% sodium Pp i , 0.1% bovine serum albumin, 0.5% SDS, 100 mM NaCl, and 0.1 mM sodium citrate, pH 7.2, at 65° C. for 2 days and then washed with a solution containing 0.1% SDS, 100 mM NaCl, and 10 mM Na-citrate, pH 7.2.
  • the same dot blot is used for hybridization with both control and experimental cDNAs, with an alkaline probe stripping procedure (soaked twice in 0.25M NaOH-0.75 M NaCl at room temperature, 30 min each, neutralized, and completely dried at 65° C. for at least 30 min) between the two hybridizations. Quantification may be done with the Betascope 603 blot analyzer (Betagen Corp.).
  • another object of the invention consists in a method for detecting the presence of mycobateria in a biological sample comprising the steps of:
  • the invention further deals with a method for detecting the presence of mycobacteria in a biological sample comprising the steps of:
  • Another object of the invention consists in a method for detecting the presence of mycobacteria in a biological sample comprising the steps of:
  • said methods comprise an additional step wherein before step a), the nucleic acid molecules of the biological sample have been made available to a hybridization reaction.
  • said methods comprise an additional step, wherein, before the detection step, the nucleic acid molecules that are not hybridized with the immobilized purified polynucleotide are removed.
  • kits for detecting mycobacteria in a biological sample comprising:
  • the invention also pertains to a kit for detecting a mycobacteria in a biological sample comprising:
  • kits for detecting mycobacteria in a biological sample comprising:
  • the invention embraces also a method for detecting the presence of a genomic DNA, a cDNA or a mRNA of mycobacteria in a biological sample, comprising the steps of:
  • the invention also provides a kit for detecting the presence of genomic DNA, cDNA or mRNA of a mycobacterium in a biological sample, comprising:
  • the recombinant BAC vectors according to the invention and the polynucleotide inserts contained therein may be used for performing detection methods based on molecular combing .
  • Said methods consist in methods for aligning macromolecules, especially DNA and are applied to processes for detecting, for measuring intramolecular distance, for separating and/or for assaying a macromolecule, especially DNA in a sample.
  • the latters When performing the molecular combing method with the recombinant BAC vectors according to the inventions or their polynucleotide inserts, the latters may be immobilized ( anchored ) on a suitable substrate and aligned as described in the PCT Application No. PCT/FR 95/00165, the whole teachings of this PCT Application being herien incorporated by reference. Then, polynucleotides to be tested, preferably under the form of radioactively or non radioactively labeled polynucleotides, that may consist of fragments of genomic DNA, cDNA etc. are brought into contact with the previously aligned polynucleotides according to the present invention and then their hybridization position on the aligned DNA molecules is determined using any suitable means including a microscope or a suitable camera device.
  • the present invention is also directed to a method for the detection of the presence of a polynucleotide of mycobacterial origin in a biological sample and/or for physical mapping of a polynucleotide on a genomic DNA, said method comprising:
  • the invention finally provides for a kit for performing the above method, comprising:
  • BAC vector preparation pBeloBAC11 was kindly provided by Dr. Shizuya, Department of Biology, California Institute of Technology (Pasadena, Calif.). The preparation followed the description of Woo et al., 1994 (Woo et al., 1994).
  • Partial digestion with HindIII Partial digestion was carried out on plugs, each containing approximately 10 ⁇ g of high molecular weight DNA, after three one hour equilibration steps in 50 ml of HindIII 1 ⁇ digestion buffer (Boehringer Mannheim, Mannheim, Germany) plus 0.1% Triton X-100. The buffer was then removed and replaced by 1 ml/plug of ice-cold HindIII enzyme buffer containing 20 units of HindIII (Boehringer). After two hours incubation on ice, the plugs were transferred to a 37° C. water bath for 30 minutes. Digestions were stopped by adding 500 ⁇ l of 50 mM EDTA (pH 8.0).
  • the partially digested DNA was subjected to contour-clamped homogenous electric field (CHEF) electrophoresis on a 1% agarose gel using a BioRad DR III apparatus (BioRad, Hercules, Calif.) in 1 ⁇ TAE buffer at 13° C., with a ramp from 3 to 15 seconds at 6 V/cm for 16 hours.
  • Agarose slices from 25 to 75 kb, 75 to 120 kb and 120 to 180 kb were excised from the gel and stored in TE at 4° C.
  • Each clone was inoculated 3 times (2 ⁇ 200 ⁇ l and 1 ⁇ 100 ⁇ l of 2YT/12.5 ⁇ g/ml CM per clone) and incubated overnight.
  • Clones containing inserts were then designated “Rv” clones, repicked from the master plate to a second set of plates for storage of the library at ⁇ 80° C.
  • the frozen pellet was resuspended in 4 ml of Solution A (50 mM glucose, 10 mM EDTA, 25 mM Tris, pH 8.0) and 4 ml of freshly prepared solution B (0.2 M NaOH, 0.2% SDS) was then added. The solution was gently mixed and kept at room temperature for 5 minutes before adding 4 ml of ice-cold solution C (3M Sodium Acetate, pH 4.7). Tubes were kept on ice for 15 min, and centrifuged at 10,000 rpm for 15 min. After isopropanol precipitation, the DNA pellet was dissolved in 600 ⁇ l RNase solution (15 mM Tris HCl pH 8.0, 10 ⁇ g/ml RNase A). After 30 minutes at 37° C.
  • Solution A 50 mM glucose, 10 mM EDTA, 25 mM Tris, pH 8.0
  • solution B 0.2 M NaOH, 0.2% SDS
  • DNA solution was extracted with chloroform:isoamylalcohol (24:1) and precipitated from the aqueous phase using isopropanol.
  • the DNA pellet was then rinsed with 70% ethanol air-dried and dissolved in 30 ⁇ l distilled water.
  • DNA prepared by this method was clean and concentrated enough to give good quality results by automatic sequencing (at least 300 bp of sequence).
  • an additional polyethylene glycol (PEG) precipitation step was necessary, which was performed as follows.
  • the 30 ⁇ l of DNA solution were diluted to 64 ⁇ l, mixed gently and precipitated using 16 ⁇ l 4M NaCl and 80 ⁇ l of 13% PEG 8000. After 30 min on ice the tubes were centrifuged at 4° C., the pellet carefully rinsed with 70% ethanol, air-dried and diluted in 20 ⁇ l of distilled water.
  • Insert sizes were determined by pulsed-field gel electrophoresis (PFGE) after cleavage with DraI (Promega). 100-200 ng of DNA was DraI-cleaved in 20 ⁇ l total reaction volume, following the manufacturer's recommendations, then loaded onto a 1% agarose gel and migrated using a pulse of 4 s for 15 h at 6.25 V/cm at 10° C. on an LKB-Pharmacia CHEF apparatus. Mid-range and low-range PFGE markers (New England Biolabs) were used as size standards. Insert sizes were estimated after ethidium bromide staining of gels.
  • PFGE pulsed-field gel electrophoresis
  • DOP-PCR As an alternate procedure we used partially degenerate oligonucleotides in combination with vector-specific (SP6 or T7) primers to amplify insert ends of BAC clones, following a previously published protocol for P1 clones (Liu et al., 1995).
  • the 96 clones of each plate were pooled by taking 20 ⁇ l of culture from each well and this procedure was repeated for 31 plates. Pooled cultures were centrifuged, the pellets were resuspended in sterile water, boiled for 5 minutes, centrifuged and the supernatants kept for PCRs. As an initial screening step, the 31 pools of a total of 2976 BACs, representing about two thirds of the library were tested for the presence of a specific clone using appropriate PCR primers.
  • PCR was performed using 10 ⁇ l of supernatant, 5 ⁇ l of assay buffer (100 mM b-mercaptoethanol, 600 mM Tris HCl (pH 8.8), 20 mM MgCl 2 , 170 mM (NH 4 ) 2 SO 4 ), 5 ⁇ l of Dimethylsulfoxide (DMSO), 5 ⁇ l of dNTPs (20 mM), 5 ⁇ l of water, 10 ⁇ l primer (2 ⁇ M), 10 ⁇ l inverse primer (2 ⁇ M) and 0.2 units of Taq DNA polymerase (Boehringer). 32 cycles of PCR (95° C. for 30 s, 55° C. for 1 min 30 s, 72° C.
  • assay buffer 100 mM b-mercaptoethanol, 600 mM Tris HCl (pH 8.8), 20 mM MgCl 2 , 170 mM (NH 4 ) 2 SO 4
  • DMSO Dimethylsulfoxide
  • FIG. 1A subpools were made to identify the specific clone.
  • Subpools representative for lane A of a 96 well plate were made by pooling clones 1 to 12 from lane A into a separate tube.
  • Subpools for lanes B to H were made in the same way.
  • subpools of each of the 12 rows were made, so that for one 96 well plate, 20 subpools were obtained. PCR with these 20 subpools identified the specific clone ( FIG. 1B , lower gel portion). If more than one specific clone was present among the 96 clones of one plate ( FIG. 1B , upper gel portion), additional PCR reactions had to be performed with the possible candidates (data not shown).
  • Sequence data from the automated sequencer ABI373A were transferred as binary data to a Digital Alpha 200 station or Sun SparcII station and analysed using TED, a sequence analysis program from the Staden software package (Dear et al., 1991). Proof-read sequences were compared using the BLAST programs (Altschul et al., 1990) to the M. tuberculosis H37Rv sequence databases of the Sanger Centre, containing the collected cosmid sequences (TB.dbs) and whole-genome shotgun reads (TB_shotgun_all.dbs) (http://www.sanger.ac.uk/).
  • Partial HindIII fragments of H37Rv DNA in the size range of 25 to 180 kb were ligated into pBeloBAC11 and electroporated into strain E. coli DH10B. While cloning of fractions I (25 to 75 kb) and 11 (75 to 120 kb)-gave approximately 4 ⁇ 10 4 transformants (white colonies), cloning of fraction m (120 to 180 kb) repeatedly resulted in empty clones. Parallel cloning experiments using partial HindIII digests of human DNA resulted in stable inserts for all three fractions (data not shown), suggesting that the maximum size of large inserts in BAC clones is strongly dependent on the source of the DNA.
  • BAC clones were systematically subjected to insert termini sequencing.
  • Two approaches direct sequencing of BAC DNA and PCR with degenerate oligonucleotide primers (DOP), adapted to the high G+C content of mycobacterial DNA, were used.
  • DOP degenerate oligonucleotide primers
  • a first screening phase 50 BAC clones designated Rv1 to Rv50 were analysed using both methods in parallel. Except for two clones, where the sequences diverged significantly, the sequences obtained by the two methods only differed in length. Sequences obtained directly were on average about 350 bp long and for 95% of the clones both the SP6 and T7 endsequences were obtained at the first attempt. Sequences obtained by DOP-PCR were mostly shorter than 300 bp.
  • BAC clones To detect rare BAC clones, a pooled PCR protocol was used. Primers were designed on the basis of the existing cosmid sequences and used to screen 31 pools of 96 BAC clones. When positive PCR products of the correct size were obtained, smaller subpools (of 8 or 12 clones each) of the corresponding pool were subsequently used to identify the corresponding clone ( FIG. 1 ). With this approach 20 additional BACs (Rv401-Rv420) were found for the regions where no BACs were found with the initial systematic sequencing approach. The endsequences of these BACs (Rv401-420) were determined by direct sequencing, which confirmed the predicted location of the clones on the chromosome.
  • tuberculosis L11920 I ponA Rv273T7 Penicillin binding M. leprae S82044 protein pstC Rv103T7 Putative M. tuberculosis Z48057 phosphate transport receptor recA Rv415SP6 Homologous M. tuberculosis X58485 recombination wag9 Rv35SP6 35-kDa antigen M. tuberculosis M69187
  • the minimal overlapping set of BAC clones represents a powerful tool for comparative genomics. For example, with each BAC clone containing on average an insert of 70 kb, it should be possible to cover a 1 Mb section of the chromosome with 15 BAC clones. Restriction digests of overlapping clones can then be blotted to membranes, and probed with radiolabelled total genomic DNA from, for example, M. bovis BCG Pasteur. Restriction fragments that fail to hybridize with the M. bovis BCG Pasteur DNA must be absent from its genome, hence identifying polymorphic regions between M. bovis BCG Pasteur and M. tuberculosis H37Rv. The results of such an analysis with clone Rv58 ( FIG.
  • FIG. 4 presents the results of this analysis, where it is clear that several restriction fragments from clone Rv58 failed to hybridize with genomic DNA from either M. bovis or M. bovis BCG Pasteur.
  • a restriction map of the polymorphic region was established and compared to the H37Rv sequence data.
  • the localization of the polymorphism could therefore be estimated, and appropriate oligonucleotide primers (Table 1) were selected for the amplification and sequencing of the corresponding region in M. bovis .
  • the alignment of M. bovis and M. tuberculosis H37Rv sequences showed that 12,732 bp were absent from the chromosomal region of the M. bovis type strain and M. bovis BCG Pasteur strain.
  • the G+C content of the polymorphic region is 62.3 mol %, which is the same as the average genome G+C content of the M.
  • ORF 6 MTCY277.33
  • the novel M. bovis sequence of the polymorphic region was deposited under accession number AJ003103 in the EMBL Nucleotide Sequence Data Library.
  • RvXXXIS 1081 corresponds to a region located close to a copy of the IS1081 repetitive sequence (Insertion element)
  • the character ⁇ - denotes an uncertain base residue.

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Abstract

A method for isolating a polynucleotide of interest that is present in the genome of and/or expressed by a first mycobacterium strain that is also absent or altered in the genome of and/or not expressed by a second mycobacterium strain. The method includes contacting the genomic DNA of the first strain under hybridizing conditions with the DNA of at least one clone belonging to a bacterial artificial chromosome (BAC) genomic DNA library of the second strain and isolating the polynucleotide of interest that does not hybridize with the DNA of the second strain. This invention further pertains to a Mycobacterium tuberculosis strain H37Rv genomic DNA library, a Mycobacterium bovis BCG strain Pasteur genomic DNA library, and the recombinant BAC vectors belonging to those genomic DNA libraries. This invention also relates to mycobacterial nucleic acids, and methods and kits for using these nucleic acids to detect mycobacteria in a biological sample.

Description

    I. BACKGROUND OF THE INVENTION
  • The present invention pertains to a method for isolating a polynucleotide of interest that is present in the genome of a mycobacterium strain and/or is expressed by said mycobacterium strain and that is absent or altered in the genome of a different mycobacterium strain and/or is not expressed in said different mycobacterium strain, said method comprising the use of at least one clone belonging to a genomic DNA library of a given mycobaterium strain, said DNA library being cloned in a bacterial artificial chromosome (BAC). The invention concerns also polynucleotides identified by the above method, as well as detection methods for mycobacteria, particularly Mycobacterium tuberculosis, and kits using said polynucleotides as primers or probes. Finally, the invention deals with BAC-based mycobacterium DNA libraries used in the method according to the invention and particularly BAC-based Mycobacterium tuberculosis and Mycobacterium bovis BCG DNA libraries.
  • Radical measures are required to prevent the grim predictions of the World Health Organisation for the evolution of the global tuberculosis epidemic in the next century becoming a tragic reality. The powerful combination of genomics and bioinformatics is providing a wealth of information about the etiologic agent, Mycobacterium tuberculosis, that will facilitate the conception and development of new therapies. The start point for genome sequencing was the integrated map of the 4.4 Mb circular chromosome of the widely-used, virulent reference strain, M. tuberculosis H37Rv and appropriate cosmids were subjected to systematic shotgun sequence analysis at the Sanger Centre.
  • Cosmid clones (Balasubramanian et al., 1996; Pavelka et al., 1996) have played a crucial role in the M. tuberculosis H37Rv genome sequencing project. However, problems such as under-representation of certain regions of the chromosome, unstable inserts and the relatively small insert size complicated the production of a comprehensive set of canonical cosmids representing the entire genome.
    • II. SUMMARY OF THE INVENTION
  • In order to avoid the numerous technical constraints encountered in the state of the art, as decribed hereabove, when using genomic mycobacterial DNA libraries constructed in cosmid clones, the inventors have attempted to realize genomic mycobacterial DNA libraries in an alternative type of vectors, namely Bacterial Artificial Chromosome (BAC) vectors.
  • The success of this approach depended on whether the resulting BAC clones could maintain large mycobacterial DNA inserts. There are various reports describing the successful construction of a BAC library for eucaryotic organisms (Cai et al., 1995; Kim et al., 1996; Misumi et al., 1997; Woo et al., 1994; Zimmer et al., 1997) where inserts up to 725 kb (Zimmer et al., 1997) were cloned and stably maintained in the E. coli host strain.
  • Here, it is shown that, surprisingly, the BAC system can also be used for mycobacterial DNA, as 70% of the clones contained inserts in the size of 25 to 104 kb.
  • This is the first time that bacterial, and specifically mycobacterial, DNA is cloned in such BAC vectors.
  • In an attempt to obtain complete coverage of the genome with a minimal overlapping set of clones, a Bacterial Artificial Chromosome (BAC) library of M. tuberculosis was constructed, using the vector pBeloBAC11 (Kim et al., 1996) which combines a simple phenotypic screen for recombinant clones with the stable propagation of large inserts (Shizuya et al., 1992). The BAC cloning system is based on the E. coli F-factor, whose replication is strictly controlled and thus ensures stable maintenance of large constructs (Willets et al., 1987). BACs have been widely used for cloning of DNA from various eucaryotic species (Cat et al., 1995; Kim et al., 1996; Misumi et al., 1997; Woo et al., 1994; Zimmer et al., 1997). In contrast, to our knowledge this report describes the first attempt to use the BAC system for cloning bacterial DNA.
  • A central advantage of the BAC cloning system over cosmid vectors used in prior art is that the F-plasmid is present in only one or a maximum of two copies per cell, reducing the potential for recombination between DNA fragments and, more importantly, avoiding the lethal overexpression of cloned bacterial genes. However, the presence of the BAC as just a single copy means that plasmid DNA has to be extracted from a large volume of culture to obtain sufficient DNA for sequencing and it is described here in the examples a simplified protocol to achieve this.
  • Further, the stability and fidelity of maintenance of the clones in the BAC library represent ideal characteristics for the identification of genomic differences possibly responsible for phenotypic variations in different mycobacterial species.
  • As it will be shown herein, BACs can be allied with conventional hybridization techniques for refined analyses of genomes and transcriptional activity from different mycobacterial species.
  • Having established a reliable procedure to screen for genomic polymorphisms, it is now possible to conduct these comparisons on a more systematic basis than in prior art using representative BACs throughout the chromosome and genomic DNA from a variety of mycobacterial species.
  • As another approach to display genomic polymorphisms, the inventors have also started to use selected H37Rv BACs for “molecular combing” experiments in combination with fluorescent in situ hybridization (Bensimon et al., 1994; Michalet et al., 1997). With such technique the one skilled in the art is enabled to explore the genome of mycobacteria in general and of M. tuberculosis in particular for further polymorphic regions.
  • The availability of BAC-based genomic mycobacterial DNA libraries constructed by the inventors have allowed them to design methods and means both useful to identify genomic regions of interest of pathogenic mycobacteria, such as Mycobacterium tuberculosis, that have no counterpart in the corresponding non-pathogenic strains, such as Mycobacterium bovis BCG, and useful to detect the presence of polynucleotides belonging to a specific mycobacterium strain in a biological sample.
  • By a biological sample according to the present invention, it is notably intended a biological fluid, such as plasma, blood, urine or saliva, or a tissue, such as a biopsy.
  • Thus, a first object of the invention consists of a method for isolating a polynucleotide of interest that is present in the genome of a mycobacterium strain and/or is expressed by said mycobacterium strain and that is absent or altered in the genome of a different mycobacterium strain and/or is not expressed in said different mycobacterium strain, said method comprising the use of at least one clone belonging to a genomic DNA library of a given mycobaterium strain, said DNA library being cloned in a bacterial artificial chromosome (BAC).
  • The invention is also directed to a polynucleotide of interest that has been isolated according to the above method and in partoular a polynucleotide containing one or several Open Reading Frames (ORFs), for example ORFs encoding either a polypeptide involved in the pathogenicity of a mycobacterium strain or ORFs encoding Polymorphic Glycine Rich Sequences (PGRS).
  • Such polynucleotides of interest may serve as probes or primers in order to detect the presence of a specific myobacterium strain in a biological sample or to detect the expression of specific genes in a particular mycobacterial strain of interest.
  • The BAC-based genomic mycobacterial DNA libraries generated by the present inventors are also part of the invention, as well as each of the recombinant BAC clones and the DNA insert contained in each of said recombinant BAC clones.
  • The invention also pertains to methods and kits for detecting a specific mycobacterium in a biological sample using either at least one recombinant BAC clone or at least one polynucleotide according to the invention, as well as to methods and kits to detect the expression of one or several specific genes of a given mycobacterial strain present in a biological sample.
    • III. BRIEF DESCRIPTION OF THE FIGURES
  • In order to better understand the present invention, reference will be made to the appended figures which depicted specific embodiments to which the present invention is in no case limited in scope with.
  • FIG. 1: PCR-screening for unique BAC clones with specific primers for 2 selected genomic regions of the H37Rv chromosome, using 21 pools representating 2016 BACs (Panel A) and sets of 20 subpools from selected positive pools (Panel B).
  • FIG. 2: Pulsed-field gel electrophoresis gel of DraI-cleaved BAC clones used for estimating the insert sizes of BACs.
  • FIG. 3: Minimal overlapping BAC map of M. tuberculosis H37Rv superimposed on the integrated physical and genetic map established by Philipp et al. (18). Y- and I-numbers show pYUB328 (2) and pYUB412 (16) cosmids which were shotgun sequenced during the H37Rv genome sequencing project. Y-cosmids marked with * were shown in the integrated physical and genetic map (18). Rv numbers show the position of representative BAC clones relative to sequenced Y- and I-clones. Squared Rv numbers show BACs which were shotgun sequenced at the Sanger Centre.
  • FIG. 4: Ethidium bromide stained gel (Panel A) and corresponding Southern blot (Panel B) of EcoRI and PvuII digested Rv58 DNA hybridized with 32P labeled genomic DNA preparations from M. tuberculosis H37Rv, M. bovis ATCC 19210 and M. bovis BCG Pasteur.
  • FIG. 5: Organisation of the ORFs in the 12.7 kb genomic region present in M. tuberculosis H37Rv but not present in M. bovis ATCC 19210 and M. bovis BCG Pasteur. Arrows show the direction of transcription of the putative genes. Positions of EcoRI and PvuII restriction sites are shown. Vertical dashes represent stop codons. The 11 ORFs correspond to the ORFs MTCY277.28 to MTCY277.38/accession number Z79701-EMBL Nucleotide Sequence Data Library. The junction sequences flanking the polymorphic region are shown.
  • FIG. 6: Variation in the C-terminal part of a PE-PGRS open reading frame in M. tuberculosis strain H37Rv relative to M. bovis BCG strain Pasteur.
  • The numbers on the right side of the Figure denote the position of the end nucleotides, taking as the reference the M. tuberculosis genome.
  • FIG. 7: Polynucleotide sequence next to the HindIII cloning site in the BAC vector pBeloBAC11 (Kim et al., 1996) used to clone the inserts of the BAC-based myobacterial genomic DNA library according to the invention.
    • NotI: location of the NotI restriction sites.
    • Primer T7-BAC1: nucleotide region recognized by the T7-BAC 1 primer shown in Table 1.
    • T7 promoter: location of the T7 promoter region on the pBeloBac11 vector.
    • Primer T7-Belo2: nucleotide region recognized by the T7-Belo2 pimer shown in Table 1.
    • Hind III: the HindIII cloning site used to clone the genomic inserts in the pBeloBAC11 vector.
    • SP6-Mid primer: nucleotide region recognized by the SP6 Mid primer shown in Table 1.
    • SP6-BAC1 primer: nucleotide region recognized by the SP6 BAC1 primer shown in Table 1.
    • SP6 promoter location of the SP6 promoter region on the pBeloBac11 vector.
    IV. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS
  • As already mentioned hereinbefore, the present invention is directed to a method for isolating a polynucleotide of interest that is present in the genome of a mycobacterium strain and/or is expressed by said mycobacterium strain and that is absent or altered in the genome of a different mycobacterium strain and/or is not expressed in said different mycobacterium strain, said method comprising the use of at least one clone belonging to a genomic DNA library of a given mycobaterium strain, said DNA library being cloned in a bacterial artificial chromosome (BAC).
  • For this purpose, the inventors have constructed several BAC-based mycobacterial genomic DNA libraries that may be used in order to perform the above described method.
  • Because it is the first time that mycobacterial genomic DNA has been successfully cloned in BAC type vectors, and because these DNA libraries are then novel and nonobvious, an object of the present invention consists in a myobacterial genomic DNA library cloned in such a BAC type vector.
  • As an illustrative example, a BAC-based DNA library of Mycobacterium tuberculosis has been realized. Forty-seven cosmids chosen from the integrated map of the 4.4 Mb circular chromosome (Philipp et al., 1996a) were shotgun-sequenced during the initial phase of the H37Rv genome sequence project. The sequences of these clones were used as landmarks in the construction of a minimally overlapping BAC map. Comparison of the sequence data from the termini of 420 BAC clones allowed us to establish a minimal overlapping BAC map and to fill in the existing gaps between the sequence of cosmids. As well as using the BAC library for genomic mapping and sequencing, we also tested the system in comparative genomic experiments in order to uncover differences between two closely related mycobacterial species. As shown in a previous study (Philipp et al., 1996b), M. tuberculosis, M. bovis and M. bovis BCG, specifically BCG Pasteur strain, exhibit a high level of global genomic conservation, but certain polymorphic regions were also detected. Therefore, it was of great interest to find a reliable, easy and rapid way to exactly localize polymorphic regions in mycobacterial genomes using selected BAC clones. This approach was validated by determining the exact size and location of the polymorphisms in the genomic region of DraI fragment Z4 (Philipp et al., 1996b), taking advantage of the availability of an appropriate BAC clone covering the polymorphic region and the H37Rv genome sequence data. This region is located approximately 1.7 Mb from the origin of replication.
  • The Bacterial Artificial Chromosome (BAC) cloning system is capable of stably propagating large, complex DNA inserts in Escherichia coli. As part of the Mycobacterium tuberculosis H37Rv genome sequencing project, a BAC library was constructed in the pBeloBAC11 vector and used for genome mapping, confirmation of sequence assembly, and sequencing. The library contains about 5000 BAC clones, with inserts ranging in size from 25 to 104 kb, representing theoretically a 70 fold coverage of the M. tuberculosis genome (4.4 Mb). A total of 840 sequences from the T7 and SP6 termini of 420 BACs were determined and compared to those of a partial genomic database. These sequences showed excellent correlation between the estimated sizes and positions of the BAC clones and the sizes and positions of previously sequenced cosmids and the resulting contigs. Many BAC clones represent linking clones between sequenced cosmids, allowing fall-coverage of the H37Rv chromosome, and they are now being shotgun-sequenced in the framework of the H37Rv sequencing project. Also, no chimeric, deleted or rearranged BAC clones were detected, which was of major importance for the correct mapping and assembly of the H37Rv sequence. The minimal overlapping set contains 68 unique BAC clones and spans the whole H37Rv chromosome with the exception of a single gap of ˜150 kb. As a post-genomic application, the canonical BAC set was used in a comparative study to reveal chromosomal polymorphisms between M. tuberculosis, M. bovis and M. bovis BCG Pasteur, and a novel 12.7 kb segment present in M. tuberculosis but absent from M. bovis and M. bovis BCG was characterized. This region contains a set of genes whose products show low similarity to proteins involved in polysaccharide biosynthesis. The H37Rv BAC library therefore provides the one skilled in the art with a powerful tool both for the generation and confirmation of sequence data as well as for comparative genomics and a plurality of post-genomic applications.
  • The above described BAC-based Mycobacterium tuberculosis genomic DNA library is part of the present invention and has been deposited in the Collection Nationale de Cultures de Microorganismes (CNCM) on Nov. 19, 1997 under the accession number I-1945.
  • Another BAC-based DNA library has been constructed with the genomic DNA of Mycobacterium bovis BCG, Pasteur strain, and said DNA library has been deposited in the Collection Nationale de Cultures de Microorganismes (CNCM) on XX XX, 1998 under the accession number I-XXXX.
  • Thus, as a specific embodiment of the above described method for isolating a polynucleotide of interest said method makes use of at least one BAC-based DNA library that has beeen constructed from the genomic DNA of Mycobacterium tuberculosis, more specifically of the H37Rv strain and particularly of the DNA library deposited in the accession number I-1945.
  • In another specific embodiment of the above described method for isolating a polynucleotide of interest said method makes use of at least one BAC-based DNA library has beeen constructed from the genomic DNA of Mycobacterium bovis BCG, more specifically of the Pasteur strain and particularly of the DNA library deposited in the accession number I-XXXX.
  • In more details, the method according to the invention for isolating a polynucleotide of interest may comprise the following steps:
      • a) Isolating at least one polynucleotide contained in a clone of a BAC-based DNA library of mycobacterial origin;
      • b) Isolating:
        • at least one genomic or cDNA polynucleotide from a mycobacterium, said mycobacterium belonging to a strain different from the strain used to construct the BAC-based DNA library of step a); or alternatively
        • at least one polynucleotide contained in a clone of a BAC-based DNA library prepared from the genome of a mycobacterium that is different from the mycobacterium used to construct the BAC-based DNA library of step a);
      • c) Hybridizing the at least one polynucleotide of step a) to the at least one polynucleotide of step b);
      • d) Selecting the at least one polynucleotide of step a) that has not formed a hybrid complex with the at least one polynucleotide of step b);
      • e) Characterizing the selected polynucleotide.
  • Following the above procedure, the at least one polynucleotide of step a) may be prepared as follows:
      • 1) Digesting at least one recombinant BAC clone by an appropriate resctriction endonuclease in order to isolate the polynucleotide insert of interest from the vector genetic material;
      • 2) Optionally amplifying the resulting polynucleotide insert;
      • 3) Optionally digesting the polynucleotide insert of step 1) or step 2) with at least one restriction endonuclease.
  • The above method of the invention allows the one skilled in the art to perform comparative genomics between different strains or species of mycobacteria cells, for example between pathogenic strains or species and their non pathogenic strains or species counterparts, as it is the illustrative case for the genomic comparison between Mycobacterium tuberculosis and Mycobacterium bovis BCG that is described herein in the examples.
  • Restriction digests of a given clone of a BAC library according to the invention may be blotted to membranes, and then probed with radiolabeled DNA form another strain or another species of mycobacteria, allowing the one skilled in the art to identify, characterize and isolate a polynucleotide of interest that may be involved in important metabolical and/or physiological pathways of the mycobacterium under testing, such as a polynucleotide functionally involved in the pathogenicity of said given mycobacteria for its host organism.
  • More specifically, the inventors have shown in Example 6 that when restriction digests of a given clone of the BAC library identified by the CNCM accession number I-1945 are blotted to membranes and then probed with radiolabeled total genomic DNA from, for example, Mycobacterium bovis BCG Pasteur, it is observed that restriction fragments that fail to hybridize with the M. bovis BCG Pasteur DNA are absent from its genome, hence identifying polymorphic regions between M. bovis BCG Pasteur and M. tuberculosis H37Rv.
  • Thus, a further object of the present invention consists in a polynucleotide of interest that has been isolated according to the method described herein before.
  • In Example 6, a polynucleotide of approximately 12.7 kilobases has been isolated that is present in the genome of M. tuberculosis but is absent of the genome of M, bovis BCG. This polynucleotide of interest contains 11 ORFs that may be involved in polysaccharide biosynthesis. In particular, two of said ORFs are of particular interest, namely ORF6 (MTCY277.33; Rv1511) that encodes a protein that shares significant homology with bacterial GDP-D-mannose dehydratases, whereas the protein encoded by ORF7 (MTCY277.34; Rv1512) shares significant homology with a nucleotide sugar epimerase. As polysaccharide is a major constituent of the mycobacterial cell wall these deleted genes may cause the cell wall of M. bovis BCG to differ from that of M. tuberculosis, a fact that may have important consequences for both the immune response to M. bovis BCG and virulence. Detection of such a polysaccharide is of diagnostic interest and possibly useful in the design of tuberculosis vaccines.
  • Consequently, the polynucleotide of interest obtained following the method according to the invention may contain at least one ORF, said ORF preferably encoding all or part of a polypeptide involved in an important metabolical and/or physiological pathway of the mycobacteria under testing and more specifically all or part of a polypeptide that is involved in the pathogenicity of the mycobacteria under testing, such as for example Mycobacterium tuberculosis, and more generally mycobacteria belonging to the Mycobacterium tuberculosis complex.
  • The Mycobacterium tuberculosis complex has its usual meaning, i.e. the complex of mycobacteria causing tuberculosis which are Mycobacterium tuberculosis, Mycobacterium bovis, Mycobacterium africanum, Mycobacterium microti and the vaccine strain Mycobacterium bovis BCG.
  • An illustrative polynucleotide of interest according to the present invention comprises all or part of the polynucleotide of approximately 12.7 kilobases that is present in the genome of M. tuberculosis but is absent from the genome of M. bovis BCG disclosed hereinbefore. This polynucleotide is contained in clone Rv58 of the BAC DNA library I-1945.
  • Generally, the invention also pertains to a purified polynucleotide comprising the DNA insert contained in a recombinant BAC vector belonging to a BAC-based mycobacterial genomic DNA library, such as for example the I-1945 BAC DNA library.
  • Advantageously, such a polynucleotide has been identified according to the method of the invention.
  • Such a polynucleotide of interest may be used as a probe or a primer useful for specifically detecting a given mycobacterium of interest, such as Mycobacterium tuberculosis or Mycobacterium bovis BCG.
  • More specifically, the invention then deals with a purified polynucleotide useful as probe or a primer comprising all or part of the nucleotide sequence SEQ ID No1:
    ACCTGCGCTT GCAGAGATCA AATAGGGCGC ATGGGTCAGC 60
    ATAGTACAGG TCGTCGCGCA
    TCTTTGATGC ATCGGAATAA GATGTCAGGC AATTAAAAGA 120
    GAAGCCACGG CGACTCGCGG
    CATTCAGCAT GTCGAGCGTC GCTTCGATGT GAGCGCACCA 180
    TTCCGTGTCC AACGATTTCA
    GACGAACATT GAATATTCCA CTCGCGACGC TATAGTCCGC 240
    CTCCCGATCT ATGCGCGCCG
    CGCAGATGAA GTCTGCGTTC GCCCGACCTT CGAAACGTAG 300
    TGCGGCCGCG CGCACCATTT
    CGGGGGAGAC GTCGATGCCG GTGTAATCAG TTTTGAAGCC 360
    ACGCGCATCT AGGTAGTCCA
    GTAGAGCCCC ATAGCCACAG CCTAGATCGT TGATCGAAAA 420
    TGGGTCCGCC GCATTGACAA
    TGCGCACCAG CTGGTCAAAG CGCAACGCCT GCCCGGCTTC 480
    GCCGTTCCAA TCGACGCCGC
    GCGGGTGCCG TGTGCTTCGA GTTTCGATGC GTAGTAACGG 540
    GCCACGTCAG CGAGCATGGT
    CGTTGCGTCT TCCGCCATGA AGCTGCCTCA CGATTTGTGT 600
    GTGTGGGCGT CGGTGCGTGG
    GTCCGAGACT ATACCTTCAA CAGTTGCATG CCGAGGCTGC 660
    GGCGGGCAAT GACCCAAAAA
    CCCGCCGGCA CGGTTCGCCG AGCAAGGAAG CGTGGAGACG 720
    ATAGATAATT TCACTGGCGA
    CAGTACCTCA AATAGTCCGG AGCCTCGGCT CCGACGTTAA 780
    AGAGCAGATC CAGAATCGAC
    ACGGCGGGCT CGAACCCTCC CCACAATTGC TTATAATCGC 840
    GGTAGCCGTC ATAATCGAAC
    CAAGTTACCC GGATGCTAAG TTCGTCGAAC ACGCGCTCAT 900
    CGACATACGA ACGGGCTGAG
    GGGCCAGAGA CATATTCGGT CGCTGCGGCC TGTTGGCAGA 960
    GGTTGGCCAG TCTCTCGGTC
    TTGCCGTCGG CTAATTCGTA GTCCCACGAA TTTGCCAGTC 1020
    GCGTGCTGAT ACCGAGATAA
    CTGCAAATCG CATTCAATAG ACGCCTGTTG AGTAAGGAAA 1080
    GATTCGTGTG CTGTTCTTCG
    AGGTAAATCG GCGCGAGCCA GTCAGCGATC TCCGCAAAAT 1140
    GAGCGGCCGC GCTGTAGTTG
    AATTCTAGTG CCCGCCAGTG CGCTTTCGCC CAATCGGTGC 1200
    CGTCGATCAG CGTCTCACGT
    ATCTTTTGAT GGAAACGTCC CTTCACCTGG ACGGGAACAG 1260
    TTATCCACTG TAACCCCTGG
    CTCGTTTTGA TCCGATTTCT GTTTCGCCAA TCACGCTTGG 1320
    TATATTGCAT GTCATCATAG
    ATGATGAATT CATCGACGAA TGCAATCAGG TCAAAATATC 1380
    CTCGCCAAGG TATGTAATTT
    GATTGAACAA TCGCGACTTT CTTCAACGCG GTGTCTCCAA 1440
    TTTAGAATAA CAAATACGTC
    GCGCCCGCGA CAGCTCCGCT GGAGCGAGTT CAAGCGATTC 1500
    TGCGACATAT TCAATATGGT
    GCTCGGGAAG GCCAGGATGG GCCGCGACCC GGGGCGTCCG 1560
    GTGCGCGATG AACGTCGCAT
    CGTCTCCTGT GAGATAATTG CATCCGATCA TATAGGGCTG 1620
    GCTGCGGCTA GGTTGCTGGC
    AAAAAGATAT CGCGGCCGAT CCGTTTCTGG TTTTGTCTTG 1680
    ATGATCAAAT CCGCTTCCGT
    TCACGAGATC GATTCCTGGT CTTCCCCCAG CGTCGCGATG 1740
    TCGATAGGTG TCGCGCTTTG
    TTCGTACCCG CACTACGCGG CGGCGAGAAC CTCGCCACCG 1800
    AATCGGGATT GGGGGGAGGA
    TACCACTCGG TCGAGGCCCG TCACCGGCCT TCTAGCGGGT 1860
    TGACCATCAG TGTTTGCAGG
    GCCCTATCCC GGTATGGCGC ACCACGGGAT CGGCAGCGTT 1920
    CCGGTTGCTG GCGTGGTACC
    TCGTTGTGGC GCCGTGGTCC ATGTCGATTG AGTGCGTGGA 1980
    TCAGTGTAAA CCGTTGCGCG
    CCATGTTCTG TAGGCACTGG TTCGGGTTGT GGTTAGGCTG 2040
    CACGGTTGGC AGGTTACCAA
    CCACTGAGCC CCTGGGCGGA TGTGAGCTCG GACTCCGCCT 2100
    ATGGGGTGTA ATTTTGGCAG
    ATTGGGCCGG GTCCCCGTGG TGAGGACTCC TCAACCGGAT 2160
    TGGGTAAGCA TGAGGTGGTG
    CTGGCAGCGG TGTCCTGGTC GCTCTCCCGA GTAGGCCCGT 2220
    TGTGACTGTC ATGTGGGCGA
    GCGGGTTTGC GCGCGTAGGA GACGATGATT ACTACGCACG 2280
    TGACCAACCA CAAGAACGGT
    GCCCATGTCA CCGTGGTGAA AACGAGTGGC GTGGTACCGA 2340
    CTACCCCTTT GGCTCCCAGC
    TGTCCATAGA GCGGCACGTA GAACGGCTGG CCCGGGACCG 2400
    CGACGTTGAC GATGCTCAGC
    GCCACGGCCA AACTCACGCA GACGCCGACC GCGCGGCGGC 2460
    GGTCTCCATG GGCTGCGAGT
    TGGTCGAATA TCCCAGCACC AGGAGGCCCG TTGGGCTCTC 2520
    GGGCTACCAG TGCAGCGATT
    GGCAAGACGA AAACGAGATA GTAGAAGGCG ACGTCCGCGG 2580
    GGGAGAAGGT GGCGGTGGCG
    AGCAACACAA TCCCCACCAT GACAGGCGGG ATACGGCGTC 2640
    CGAGCGCCAG CACGGCGACC
    ACGACTATGA CTAGGACAGC AAACCCGATC TGCGTTCGCG 2700
    GACCAGTGAG GAAACCCTCT
    GGGATCTTGC CCGATTGATA GTTCTTGATG CTATCGGGGA 2760
    TCAGCAGGAG TGCCTTGCCA
    AAGGACACGT TCCGCGGGTC TCGAAGCCCT CCGAACGAAC 2820
    TATTGAACTT GATGATGCCG
    TGGATCGACT GTGCGATCGT CCCCGGGAAG CCTCGTGGCC 2880
    ACAACAGAAA GGCTGCGATA
    TTGGACACCA CCACGCCGGT GATCCCGATA CCAGCCCACC 2940
    GCCATTGTCG AGCCGCCAAC
    AACACCACGC CGAGAACGAC GAACTGCGGC TTTACCAGGA 3000
    CGGCCAAGAT CACCGTGATG
    GTGGCGAGGC CCCACCGCTG TCGGGACAAC GCCACGAAGT 3060
    AAGCCAGCGC GATCGGTACC
    ACGAACCCTG TCGAGTTGCC TCGATCGATG ACCCCCCACG 3120
    CCGGGATGGC CGCGGCGCCC
    AGTGTCACGA AGATGACCAC TCGCTCCAGA CCACGTGCCC 3180
    CCCGGGCCGC CCAGATGGCG
    GGAGATATGA CCGCCATCGT TAGGGCGACC AGGTAACAGA 3240
    TCAGCCCCAA GCGCGGCGCA
    CCCAGCCAAT GGCTGGGTAG TCCGAAAATC GCATACGGTA 3300
    TGCGGGCGGG GGCCCATGCA
    GCAACCGCGG TCGGCTGGTA ATCGGCGGGT AGCGAGATCA 3360
    GGTAGTCCGC GGGATTGGGT
    TGAATCCCGG CGGCGGCGAC CATGGCGTAG TCGCTGAAGC 3420
    AGTGCCGACC GATATTCATG
    CCCCAATCAA GCCAACAGTC CCCAGGGACT ACCAAAAGAG 3480
    TGGAAAAGAC GTCGACCGCG
    TACCACTGAC TGAGGGCGTA CGCCGTCGCC GCCGAAATCA 3540
    CCGACGCCAG CAGGATGGTG
    CCGAGCATGA GGGTGCGCTC GGATTGGGAG CCGATCGCCC 3600
    AGAGCCGCTC CCGGCTCGCG
    GTCACGGCAC CGCGCAACAC CTCCGGGGGT CGCTTCATCT 3660
    GGATTCTCCT CGGTTCTGCG
    CGAAACGGTA GCAGAGCGCC ATGGTTGCCA ACGCGGTCGC 3720
    CGGGCAGTCT AGACCGGATC
    TTCCTCCTGG CAACCGACAA CAGGACGTCG TTGCCGAAAG 3780
    GGCGCTGGGC ACCGACATCT
    AGGATGAACC CACAGCCACG CCCCGACGTT ATGCCATGGC 3840
    GAAGAGCGAC CGGCAGGAGC
    GGGAACCCAG TGAAGCGAGC GCTCATCACC GGAATCACAG 3900
    GACCGGACGG CTCGTATCTC
    GCTAAGCTCC CGCTGAAGGG ATATGTGCCC GCTGGTAGCC 3960
    CGGCCGAGGT CTATTTCTGC
    TGGGCGACAC GGAATTATCG CGAATTGTAT GGCTTGCTCG 4020
    CGGTCAACAG CATCTGGTTC
    AATCACGAAT CACCGCGTCA CGGCGAGACA TTCATGACTC 4080
    GTAATCCTGC ACCATATCGC
    GGTCGGCAAC GAGGCGCTGA TCGATGCGCA GACGCTGATG 4140
    CGCCCGCCCA CCCGGATAGG
    TATCAGTATT GGGGCGTTCC GGCCAGCGTA CGAGGCGTGA 4200
    TCGACCGCGC AATGGGTGTT
    TGCGTTGAGT AATAATCTGA ACCGTGTGAA CGCATGCATG 4260
    GATGCATTCC TTGCCCGTAT
    CCGCTCACAT GTTGATGCGC ACGCGCCAGA ATTGCGTTCA 4320
    CTGTTCGATA CGATGGCGGC
    CGAGGCCCGA TTTGCACGCG ACTGGCTGTC CGAGGACCTC 4380
    GCGCGGTTGC CTGTCGGTGC
    AGCATTGCTG GAAGTGGGCG GGGGGGTACT TCTGCTCAGC 4440
    TGTCAACTGG CGGCGGAGGG
    ATTTGACATC ACCGCCATCG AGCCGACGGG TGAAGGTTTT 4500
    GGCAAGTTCA GACAGCTTGG
    CGACATCGTG CTGGAATTGG CTGCAGCACG ACCCACCATC 4560
    GCGCCATGCA AGGCGGAAGA
    CTTTATTTCC GAGAAGCGGT TCGACTTCGC CTTCTCGCTG 4620
    AATGTGATGG AGCACATCGA
    CCTTCCGGAT GAGGCAGTCA GGCGGGTATC GGAAGTGCTG 4680
    AAACCCGGGG CCAGTTACCA
    CTTCCTGTGC CCGAATTACG TATTCCCGTA CGAACCGCAT 4740
    TTCAATATCC CAACATTCTT
    CACCAAAGAG CTGACATGCC GGGTGATGCG ACATCGCATC 4800
    GAGGGCAATA CGGGCATGGA
    TGACCCGAAG GGAGTCTGGC GTTCGCTCAA CTGGATTACG 4860
    GTTCCCAAGG TGAAACGCTT
    TGCGGCGAAG GATGCGACGC TGACCTTGCG CTTCCACCGT 4920
    GCAATGTTGG TATGGATGCT
    GGAACGCGCG CTGACGGATA AGGAATTCGC TGGTCGCCGG 4980
    GCACAATGGA TGGTCGCTGC
    TATTCGCTCG GCGGTGAAAT TGCGTGTGCA TCATCTGGCA 5040
    GGCTATGTTC CCGCTACGCT
    GCAGCCCATC ATGGATGTGC GGCTAACGAA GAGGTAATGA 5100
    CATGGCGCAA GCGACATCGG
    GCATTCGCGC GGCACTTTCG CAACCTGCTG TGTATGAGGC 5160
    GTATCAGCGG ATTGCGGGCG
    CTAAAAGCGG GCTTGCGTGG ATCACAACCG ACCCCATCCA 5220
    GTCGTTGCCA GGCATGCGTA
    CTCTCGACCT CGGTTGCTGG CCAGCGGTGA TACACAGCTC 5280
    CCCGCCAGTG GACGTGACAT
    GTACGAGAGA CGGCATGAGC GCGGAATGTG CGACCGTGCC 5340
    GTCGAGATGA CCGACGTCGG
    CGCTACGGCA GCCCCCACCG GACCTATCGC GCGGGGCAGC 5400
    GTCGCTCGGG TCGGCGCGGC
    GACCGCGTTG GCCGTTGCCT GCGTCTACAC GGTCATCTAT 5460
    CTGGCGGCCC GCGACCTACC
    CCCGGCTTGT TTTTCGATAT TCGCGGTGTT TTGGGGGGCG 5520
    CTCGGCATTG CCACCGGCGC
    CACCCACGGC CTCCTGCAAG AAACGACCCG CGAGGTCCGC 5580
    TGGGTGCGCT CCACCCAAAT
    AGTTGCGGGC CATCGTACCC ATCCGCTGCG GGTGGCCGGG 5640
    ATGATTGGCA CCGTCGCGGC
    CGTCGTAATT GCGGGTAGCT CACCGCTGTG GAGCCGACAG 5700
    CTATTCGTCG AGGGGCGCTG
    GCTGTCCGTG GGGCTACTCA GCGTTGGGGT GGCCGGGTTC 5760
    TGCGCGCAGG CGACCCTGCT
    GGGCGCGCTG GCCGGCGTCG ACCGGTGGAC ACAGTACGGG 5820
    TCACTGATGG TGACCGACGC
    GGTCATCCGG TTGGCGGTCG CCGCGGCAGC GGTTGTGATC 5880
    GGATGGGGTC TGGCCGGGTA
    CTTGTGGGCC GCCACCGCGG GAGCGGTGGC GTGGCTGCTC 5940
    ATGCTGATGG CCTCGCCCAC
    CGCGCGCAGC GCGGCCAGCC TGCTGACGCC CGGGGGAATC 6000
    GCCACGTTCG TGCGCGGTGC
    CGCTCATTCG ATAACCGCCG CGCGTGCCAG CGCGATTCTG 6060
    GTAATGGGTT TCCCAGTGTT
    GCTCAAAGTG ACCTCCGACC AGTTAGGGGC AAAGGGCGGA 6120
    GCGGTCATCC TGGCTGTGAC
    CTTGACGCGT GCGCCGCTTC TGGTCCCACT GAGCGCGATG 6180
    CAAGGCAACC TGATCGCGCA
    TTTCGTCGAC CGGCGCACCC AACGGCTTCG GGCGCTGATC 6240
    GCACCGGCGC TGGTCGTCGG
    CGGCATCGGT GCGGTCGGGA TGTTGGCCGC AGGGCTTACC 6300
    GGTCCCTGGT TGCTGCGTGT
    TGGATTCGGC CCCGACTACC AAACTGGCGG GGCGTTGCTG 6360
    GCCTGGTTGA CGGCAGCGGC
    GGTAGCTATC GCCATGCTGA CGCTGACCGG CGCCGCCGCG 6420
    GTCGCGGCCG CACTGCACCG
    GGCGTATTTG CTGGGCTGGG TCAGCGCGAC GGTGGCGTCG 6480
    ACGCTGTTGC TGCTGCTGCC
    GATGCCGCTG GAGACGCGCA CCGTGATCGC GCTGTTGTTC 6540
    GGTCCAACGG TGGGAATCGC
    CATCCATGTG GCCGCGTTGG CGCGGCGACC CGACTGATTT 6600
    GTGCCCCAGG TCGACAAATC
    ACGCCGTCTC GTCAGTGAGC ACTCCGTCCT CGGGTCCGAT 6660
    CCTTCCAGGA GACGTTGCAA
    CCTGATTTGG CTCAAATTGG TGCGCACCGA GGGTCGGGCA 6720
    CATCGTAGGG TCGCAACAGT
    CACATGTGTC ACTGCACCGG GCGACACCCG ATGTCCCGGC 6780
    TCTCAGCGAC AGCTGTCTGA
    CCTGTGGTTT TGTTCCCAAG TTGGTCGTGG CTGTGCGGGA 6840
    TTGGAGGTGG CGTGGGGGTC
    GCGTCGTATG GATTCTCCTC CTCGGTTCCG CGCGAAACGG 6900
    CCGCAGGCGC AATGGTCACC
    AACTTGGCCG CGGTGGAGTC TAGCCTCACA TTTTCCTGGT 6960
    CGCCCCCGAC AACCAGGAGG
    TCGCTGCAGA ACGGGCGTTC CCTACCCACA TCTACTATGA 7020
    AGCGACAGCG GCGCCCCGCT
    GTGATGGCTG AGCATGACCG ACAGAGGCGG GAAGACAGTG 7080
    AAGCGAGCGC TCATCACCGG
    AATCACCGGC CAGGACGGCT CGTATCTCGC CGAACTGCTG 7140
    CTGGCCAAGG GGTATGAGGT
    TCACGGGCTC ATCCGGCGCG CTTCGACGTT CAACACCTCG 7200
    CGGATCGATC ACCTCTACGT
    CGACCCGCAC CAACCGGGCG CGCGGCTGTT TCTGCACTAT 7260
    GGTGACCTGA TCGACGGAAC
    CCGGTTGGTG ACCCTGCTGA GCACCATCGA ACCCGACGAG 7320
    GTGTACAACC TGGCGGCGCA
    GTCACACGTG CGGGTGAGCT TCGACGAACC CGTGCACACC 7380
    GGTGACACCA CCGGCATGGG
    ATCCATGCGA CTGCTGGAAG CCGTTCGGCT CTCTCGGGTG 7440
    CACTGCCGCT TCTATCAGGC
    GTCCTCGTCG GAGATGTTCG GCGCCTCGCC GCCACCGCAG 7500
    AACGAGCTGA CGCCGTTCTA
    CCCGCGGTCA CCGTATGGCG CCGCCAAGGT CTATTCCTAC 7560
    TGGGCGACCC GCAATTATCG
    CGAAGCGTAC GGATTGTTCG CCGTTAACGG CATCTTGTTC 7620
    AATCACGAAT CACCGCGGCG
    CGGTGAGACG TTCGTGACCC GAAAGATCAC CAGGGCCGTG 7680
    GCACGCATCA AGGCCGGTAT
    CCAGTCCGAG GTCTATATGG GCAATCTGGA TGCGGTCCGC 7740
    GACTGGGGGT ACGCGCCCGA
    ATACGTCGAA GGCATGTGGC GGATGCTGCA GACCGACGAG 7800
    CCCGACGACT TCGTTTTCGC
    GACCGGGCGC GGTTTCACCG TGCGTGAGTT CGCGCGGGCC 7860
    GCGTTCGAGC ATGCCGGTTT
    GGACTGGCAG CAGTACGTGA AATTCGACCA ACGCTATCTG 7920
    CGGCCCACCG AGGTGGATTC
    GCTGATCGGC GACGCGACCA AGGCTGCCGA ATTGCTGGGC 7980
    TGGAGGGCTT CGGTGCACAC
    TGACGAGTTG GCTCGGATCA TGGTCGACGC GGACATGGCG 8040
    GCGCTGGAGT GCGAAGGCAA
    GCCGTGGATC GACAAGCCGA TGATCGCCGG CCGGACATGA 8100
    ACGCGCACAC CTCGGTCGGC
    CCGCTTGACC GCGCGGCCCG GGTCTACATC GCCGGGCATC 8160
    GCGGCCTGGT CGGGTCCGCG
    CTGCTACGCA CGTTTGCGGG CGCGGGGTTC ACCAACCTGC 8220
    TGGTGCGGTC ACGCGCCGAG
    CTTGATCTGA CGGATCGGGC CGCGACGTTC GACTTCGTTC 8280
    TCGAGTCGAG GCCGCAGGTC
    GTCATCGACG CGGCGGCCCG GGTCGGCGGC ATCCTGGCCA 8340
    ACGACACCTA CCCGGCCGAT
    TTCCTGTCGG AAAACCTCCA GATCCAGGTC AACCTGCTGG 8400
    ATGCCGCCGT GGCGGCGCGG
    GTGCCGCGGC TGCTGTTCCT GGGCTCGTCG TGCATCTACC 8460
    CGAAACTCGC CCCGCAGCCG
    ATCCCGGAGA GCGCGCTGCT CACCGGTCCG TTGGAGCCGA 8520
    CCAACGACGC GTACGCGATC
    GCCAAAATCG CCGGCATCCT TGCGGTCCAG GCGGTGCGCC 8580
    GCCAACATGG CCTGCCGTGG
    ATCTCGGCGA TGCCCACCAA CCTGTACGGG CCAGGCGACA 8640
    ACTTTTCGCC GTCCGGCTCG
    CATCTGCTGC CGGCACTCAT CCGCCGCTAT GACGAGGCCA 8700
    AAGCCAGTGG CGCGCCCAAC
    GTGACCAACT GGGGCACCGG CACGCCCCGA CGGGAGTTGC 8760
    TGCACGTCGA CGACCTGGCG
    AGCGCATGCC TGTATCTGCT GGAACATTTC CACGGGCCGA 8820
    CCCATGTCAA CGTGGGAACC
    GGCATCGACC ACACCATCGG CGAGATCGCC GAGATGGTCG 8880
    CCTCGGCGGT AGGCTATAGC
    GGCGAAACCC GCTGGGATCC AAGCAAACCG GACGGAACAC 8940
    CACGCAAACT GCTGGATGTT
    TCGGTGCTAC GGGAGGCGGG ATGGCGGCCT TCGATCGCGC 9000
    TGCGCGACGG CATCGAGGCG
    ACGGTGGCGT GGTATCGCGA GCACGCGGGA ACGGTTCGGC 9060
    AATGAGGCTG GCCCGTCGCG
    CTCGGAACAT CTTGCGTCGC AACGGCATCG ACGTGTCGCG 9120
    CTACTTTGCC GAACTGGACT
    GGGAACGCAA TTTCTTGCGC CAACTGCAAT CCCATCGGGT 9180
    CAGTGCCGTG CTCGATGTCG
    GGGCCAATTC GGGGCAGTAC GCCAGGGGTC TGCGCGGCGC 9240
    GGGCTTCGCG GGCCGCATCG
    TCTCGTTCGA GCCGCTGCCC GGGCCCTTTG CCGTCTTGCA 9300
    GCGCAGCGCC TCCACGGACC
    CGTTGTGGGA ATGCCGGCGC TGTGCGCTGG GCGATGTCGA 9360
    TGGAACCATC TCGATCAACG
    TCGCCGGCAA CGAGGGCGCC AGCAGTTCCG TCTTGCCGAT 9420
    GTTGAAACGA CATCAGGACG
    CCTTTCCACC AGCCAACTAC GTGGGCGCCC AACGGGTGCC 9480
    GATACATCGA CTCGATTCCG
    TGGCTGCAGA CGTTCTGCGG CCCAACGATA TTGCGTTCTT 9540
    GAAGATCGAC GTTCAAGGAT
    TCGAGAAGCA GGTGATCGCG GGTGGCGATT CAACGGTGCA 9600
    CGACCGATGC GTCGGCATGC
    AGCTCGAGCT GTCTTTCCAG CCGTTGTACG AGGGTGGCAT 9660
    GCTCATCCGC GAGGCGCTCG
    ATCTCGTGGA TTCGTTGGGC TTTACGCTCT CGGGATTGCA 9720
    ACCCGGTTTC ACCGACCCCC
    GCAACGGTCG AATGCTGCAG GCCGATGGCA TCTTCTTCCG 9780
    GGGCAGCGAT TGACGCGCCG
    GCGCGTCAAT CTATTTCGAC ATTCGCGTGA AGACGTTTTC 9840
    CCAGAATCGA CTGTTGTAGG
    CGTAGAACTC CCGGCCGCGT AGGTAGGCAT GTGATATTCG 9900
    CCTTCCCCCG AACGGGTAGC
    GGCGATGAAG GTCGCCCATG CGGCGCAGAT CACCGAAGAC 9960
    CGCGCTTGGT TCCCGGTGCG
    AGCCGACGCC CGTGGTGTCG AACTCGCACA GCACACACCG 10020
    AATCGTGACC GGCTCGCATA
    CCAGCGCGGC CCGCAATATG AATTCCTGGT CGGCGGCGAT 10080
    CCCGAAATCA AGGTCGTAGC
    CACCGATCTT GGCCACCAGC GATGATCCGA AGAACGATGC 10140
    TTGATGCGGA ACAACCTGCT
    TGCCGGCCAG GAATTTGCGC AGGCTGAAAG GTATCGGGCC 10200
    GCGCACCCGA TCGAGCCCGA
    CGAGACGATC CATCCCGAAG CCCCACAATT CGGACACCGG 10260
    TCCCTTGCCG GATAGCGCCT
    CCACGGCCTG GGOTACCACG TCGGGCCCGG AAAAACGATC 10320
    GGCGGAGTGC AAGAACCACA
    ACAGATCACC CGATGCGTGC GCGATGCCCT GGTTCATCGC 10380
    GTCGTACCGC CCGCCGTCGG
    GCTCGGACTG CCAATACGCG AAGCCTGGTT CACACCCGGA 10440
    CAGGTATGCC ACCACGTCGT
    CGCCGCTGCC ACCGTCGATT ACGATGTGCT CGATGCGTCC 10500
    CCGGTAGCGT TGCGCCCGCA
    CACTTTTCAC CGTGCGCTGC AACCCGTCGA GGTCGTTGAA 10560
    CGAGATCGTT ATCACCGAGA
    CGGTCGGAGC AGACGTCACC GAGTTCCCCT AGGTTGCTGG 10620
    CGGCGATTGT GGATCACCGG
    GTCTTGATAC CGATGAAGGT GCCTCGAAGA TTCGCCGCAT 10680
    AGGAACCTCC GAGCAACGAC
    TCGGCGATGC TTGGTTCCAA GTTGTCGTAC TCCTCCATCA 10740
    CCAGGTCGAC GCCGACGTCT
    TTGATGGCCT GAAGTAGGTG CTCGCGTTGA ATCCAGAATG 10800
    ACCGGCGATT GTCCCAGGAC
    GCCCATTTTG CGGTGTCGCG CTGGCCAAAC GAGCGGTCGT 10860
    CGGAAAACTC GGTAAACCAC
    CTACCGGGAA GTCCCTCATG TTCGGTGGGC GCCGAGAGCA 10920
    TGAACTTCAC CGGCGCCGGC
    CGCCGCAGCA ACCGATCGGT CAATTGTCGT GCCGTCGTGG 10980
    GCAACCGGAG CCATTTATCG
    CTCCGGTTGA TGATCGAGAA GTGCGTCTGG AGAATCAGCA 11040
    GCTTGTTCGT TACCGACGAG
    AGGGTTTCCA GGTATTGCTT CGGATTCTCC AGGTGGTAGA 11100
    AGAGGCCGCA GCAGAAGACG
    GTATCGAAGA GCCCGTGGTT GGCGATGTTG AGGGCGTTGT 11160
    CGTGGACGAA CCGGAGATTC
    GGCAGGTTGG TCTTCGATTT GATGTAGTTG CAGGCCGCCA 11220
    TGTTCAGCTC GCGAACCTCG
    ATCCCGAGGA CCTGAAATCC CATGCGCGCG AACCCGACCG 11280
    CGTACCCGCC TTCCAAGCAG
    CCGACATCGG CCAGGCGTAG GTGGCTCTTG TCCCCGGGAA 11340
    AGACGGTTTC CAGAATCCCG
    CGCGCCGAGA TGAACCAGGA CGATTCGTCT AACGTGCGCG 11400
    AGGACTCCGG TATCGTCAAG
    GTTCCGTCGT CGAGGCGAAC GTTGTGGGCG GTGAATTGTA 11460
    CCGCGCCGGC CGAATGTTCC
    TGTGCCATCA CTTGGTTAGC CCCTTCGGCT GGTCCTGGGT 11520
    TTGTCGACAT GGTCAGGCTC
    GACAGCCGCG TCGGAGCCGG GAGGGCCACA CATCCACGAG 11580
    CCCCCTGCGG CTCGGCGTCG
    CGGCGGCGAG CTTGCGCCAC TGGGTCTTGA GCCGCCGCGC 11640
    GGGTGTCGCC CCGCGGTGCT
    GCAGCGCCAG CATGGCGATC CGGGGATGGC GCGCGATGGT 11700
    TTCCTGCAGC GCGGCGCGCC
    CCTCCGGGCC TGGAACGTTG GCGATCTGGC GAAGGATCCA 11760
    GTCGGCCATG ACGGCGATGA
    GCTCCTCGCG CGCGGGGTCT CCCGGGAACA GGTCGAGCAT 11820
    CGCGTCAAAC GTCGCCGCAT
    GCCCCGGACC CTGCGTCAAC CAGAACTTTG GCGGGTCCAC 11880
    CACCTGGTTG TGCCACATGC
    CTTGGGCGTG GCGGCGATAC ACGGCCATGG TGTCGGGCAA 11940
    CATGGCGATG TCGCCATGCA
    CCGCGTGCCG GACGTGCAGA TACCAGTCCA GGGGCATGAC 12000
    GTCGGCAGGA ATGTCGTCGT
    AGCGCTCGAG GCGACGGTAC ACGGCCGAGT TGGTCTGGAT 12060
    GAAGTTCATC AAGATCAACG
    CATCCAGGCT CAAGTTGCCC CGCACCCGAA CCGGGGGGAA 12120
    CTTCGAGTCC TTGGCATGGC
    CGTCCTCCCA TATCACTCGG ACGGGATGGA AGCACACCGT 12180
    CGTCTTGGGG TGCCGGTCGA
    GGAATGCGAC CTGTTTGCTT AGCTTCAGCG GATCGATCCA 12240
    GTAGTCGTCC GCCTCGCACA
    ACGCGACGTA CTCGCCGCGA GCGGCCGACA GGGCGCCGGT 12300
    CAGGTTCCCA TTGAGGCCGA
    GGTTTTCGGT CCTGAAGATC GGCCGGAACA CGTGCGGGTA 12360
    CCGCTCGGCG TACTCACGGA
    TGATCGCCGG GGTGGCATCG GTCGACGCGT CGTCGGCGAC 12420
    GATGATCTCC ACCGGGAAGT
    CGGTTTGCTG GTCGAGAAAG CTGTCGAAGG CCTGACGGGC 12480
    GTAGCCCGCC TGGTTGTGAG
    TGGTCGAGAC GATGCTCACC TTGGGGCAAA GCTGGGGACT 12540
    CACCGTCGGC CCTTTTCCTG
    CGCGGCCGCA AGGGTATTGC GATGGCGAAC GTGAATCGCC 12600
    TGTGCCCGCC GGCCGTCGGC
    CGTCGTGGCC TGGTGGTCGG CGGACGTACG GCACACGCTG 12660
    GCGAAGTATA GCGAGGGTGC
    ACTGACGTTG GGCTCGAACC GCGTGGCGCG CGGTGTGGGC 12720
    CCACCGTCTC GAGTCGGTGC
    TGGTTGGCTC GC 12732
  • The location, on the Mycobacterium tuberculosis chromosome, of the above polynucleotide of sequence SEQ ID N o1 has now been ascribed to begin, at its 5′end at nucleotide at position nt 1696015 and to end, at its 3′end, at nucleotide at position nt 1708746.
  • For diagnostic purposes, this 12.7 kb deletion should allow a rapid PCR screening of tubercle isolates to identify whether they are bovine or human strains. The primers listed in Table 1 are flanking the deleted region and give a 722 bp amplicon in M. bovis or M. bovis BCG strains, but a fragment of 13,453 bp in M. tuberculosis that is practically impossible to amplify under the same PCR conditions. More importantly, assuming that some of the gene products from this region represent proteins with antigenic properties, it could be possible to develop a test that can reliably distinguish between the immune response induced by vaccination with M. bovis BCG vaccine strains and infection with M. tuberculosis or that the products (e.g. polysaccharides) are specific immunogens.
  • The invention also provides for a purified polynucleotide useful as a probe or as a primer, said polynucleotide being chosen in the following group of polynucleotides:
    • a) a polynucleotide comprising at least 8 consecutive nucleotides of the sequence SEQ ID N o1.
    • b) a polynucleotide whose sequence is fully complementary to the sequence of the polynucleotide defined in a);
    • c) a polynucleotide that hybridizes under stringent hybridization conditions with the polynucleotide defined in a) or with the polynucleotide defined in b).
  • For the purpose of defining a polynucleotide or oligonucleotide hybridizing under stringent hybridization conditions, such as above, it is intended a polynucleotide that hybridizes with a reference polynucleotide under the following hybridization conditions:
  • The hybridization step is realized at 65° C. in the presence of 6×SSC buffer, 5× Denhardt's solution, 0.5% SDS and 100 μg/ml of salmon sperm DNA.
  • For technical information, 1×SSC corresponds to 0.15 M NaCl and 0.05M sodium citrate; 1× Denhardt's solution corresponds to 0.02% Ficoll, 0.02% polyvinylpyrrolidone and 0.02% bovine serum albumin.
  • The hybridization step is followed by four washing steps:
      • two washings during 5 min, preferably at 65° C. in a 2×SSC and 0.1% SDS buffer;
      • one washing during 30 min, preferably at 65° C. in a 2×SSC and 0.1% SDS buffer,
      • one washing during 10 min, preferably at 65° C. in a 0.1×SSC and 0.1% SDS buffer
  • A first illustrative useful polynucleotide that is included in the polynucleotide of sequence SEQ ID N o1 is the following polynucleotide of sequence SEQ ID N o2 that corresponds to the Sp6 endsequence of SEQ ID No1:
    ATACTCAAGC TTGCCGCAAT CGAAACCAAC CTGTTTGTGC 60
    CGCAAGAAAT TACGCCGTGG
    CCCGGCGCCG ATCAAGAAAC GCCCCGGCGC GCGGCGGTGT 120
    CGTCGTATGG CATGACGGGC
    ACCAATGTGC ACGCCATTGT CGAGCAGGCA CCGGTGCCAG 180
    CCCCCGAATC CGGTGCACCA
    GGCGACACCC CGGCCACACC CGGTATCGAC GGCGCGCTGC 240
    TGTTCGCGCT GTCGGCCAGC
    TCGCAGGACG CGCTGCGGCA AACCGCCGCG CGGCTGGCCG 289
    ATTGGGTCT
  • A second illustrative useful polynucleotide that is included in the polynucleotide of sequence SEQ ID N o1 is the following polynucleotide of sequence SEQ ID N o3 that corresponds to the T7 endsequence of SEQ ID N o1, located on the opposite strand:
    TTGGCGGGTT GGCCACACAC CCGCCGGTGA CGGCGACGAT 60
    GCTGGGCTGG TTGCGGCCCT
    GCGCCACCGC GGCTTGCATG CTGGTTGGCT GTCTTGGGAC 120
    GATCCCGAAA TAGTCCACGC
    GGATCTGGTG ATTTTGCGGG CTACCCGCGA TTACCCCGCG 180
    CGGCTCGACG AGTTTTTGGC
    CTGGACTACC CGCGTGGCCA ATCTGCTGAA CTCGCGGCCG 240
    GTGGTGGCCT GGAATGTCCA
    CGCCGTTCAC CTACGTGACC TTGATGGGAT 278
    CCGGGGGT
  • The polynucleotide of sequence SEQ ID N o1 contains 11 ORFs, the respective locations of which, taking into account the orientation of each ORF on the chromosome, on the sequence of the Mycobacterium tuberculosis chromosome, is given hereafter:
      • The location of ORF1 is comprised between nucleotide at position nt 1695944 and nucleotide at position nt1696441.
      • The location of ORF2 is comprised between nucleotide at position nt 1696728 and nucleotide at position nt1697420.
      • The location of ORF3 is comprised between nucleotide at position nt 1698096 and nucleotide at position nt1699892. ORF3 probably encodes a protein having the characteristics of a membrane protein.
      • The location of ORF4 is comprised between nucleotide at position nt 1700210 and nucleotide at position nt1701088.
      • The location of ORF5 is comprised between nucleotide at position nt 1701293 and nucleotide at position nt1702588. ORF5 encodes a protein having the characteristics of a membrane protein.
      • The location of ORF6 is comprised between nucleotide at position nt 1703072 and nucleotide at position nt1704091. ORF6 encodes a protein having the characteristics of a GDP-D-mannose dehydratase.
      • The location of ORF7 is comprised between nucleotide at position nt 1704091 and nucleotide at position nt1705056. ORF7 encodes a protein having the characteristics of a nucleotide sugar epimerase involved in colanic acid biosynthesis.
      • The location of ORF8 is comprised between nucleotide at position nt 1705056 and nucleotide at position nt1705784.
      • The location of ORF9 is comprised between nucleotide at position nt 1705808 and nucleotide at position nt1706593. ORF9 encodes a protein having the characteristics of colanic acid biosynthesis glycosyl transferase.
      • The location of ORF10 is comprised between nucleotide at position nt 1706631 and nucleotide at position nt1707524.
  • The location of ORF11 is comprised between nucleotide at position nt 1707530 and nucleotide at position nt1708648. ORF11 encodes a protein similar to a spore coat polysaccharide biosynthesis.
  • A polynucleotide of interest obtained by the above-disclosed method according to the invention may also contain at least one ORF that encodes all or part of acidic, glycine-rich proteins, belonging to the PE and PPE families, whose genes are often clustered and based on multiple copies of the polymorphic repetitive sequences. The names PE and PPE derive from the fact that the motifs ProGlu (PE, positions 8, 9) and ProProGlu (PPE, positions 7 to 9) are found near the N-terminus in almost all cases. The PE protein family all have a highly conserved N-terminal domain of ˜110 amino acid residues, that is predicted to have a globular structure, followed by a C-terminal segment which varies in size, sequence and repeat copy number. Phylogenetic analysis separated the PE family into several groups, the larger of which is the highly repetitive PGRS class containing 55 members whereas the other groups share very limited sequence similarity in their C-terminal domains. The predicted molecular weights of the PE proteins vary considerably as a few members only contain the ˜110 amino acid N-terminal domain while the majority have C-terminal extensions ranging in size from 100 up to >1400 residues. A striking feature of the PGRS proteins is their exceptional glycine content (up to 50%) due to the presence of multiple tandem repetitions of GlyGlyAla or GlyGlyAsn motifs or variations thereof.
  • Like the PE family, the PPE protein family also has a conserved N-terminal domain that comprises ˜180 amino acid residues followed by C-terminal segments that vary considerably in sequence and length. These proteins fall into at least three groups, one of which constitutes the MPTR class characterised by the presence of multiple, tandem copies of the motif AsnXGlyXGlyAsnXGly. The second subgroup contains a characteristic, well-conserved motif around position 350 (GlyXXSerValProXXTrp), whereas the other group contains proteins that are unrelated except for the presence of the common 180-residue PPE domain. C-terminal extensions may range in size from 00 up to 3500 residues.
  • One member of the PGRS sub-family, the WHO antigen 22T (Abou-Zeid et al., 1991), a 55 kD protein capable of binding fibronectin, is produced during disease and elicits a variable antibody response suggesting either that individuals mount different immune responses or that this PGRS-protein may not be produced in this form by all strains of M. tuberculosis. In other words, at least some PE_PGRS coding sequences encode for proteins that are involved in the recognition of M. tuberculosis by the immune system of the infected host. Therefore, differences in the PGRS sequences could represent the principal source of antigenic variation in the otherwise genetically and antigenically homogeneous bacterium.
  • By performing the method of the invention using the M tuberculosis BAC based DNA library I-1945, the inventors have discovered the occurence of sequence differences between a given PGRS encoding ORF (ORF reference on the genomic sequence of M. tuberculosis Rv0746) of M. tuberculosis and its counterpart sequence in the genome of M. bovis BCG.
  • More precisely, the inventors have determined that one ORF contained in BAC vector No Rv418 of the M. tuberculosis BCG I-1945 DNA library carries both base additions and base deletions when compared with the corresponding ORF in the genome of M. bovis BCG that is contained in the BAC vector No X0175 of the M. bovis BCG I-XXXX DNA libary. The variations observed in the base sequences correspond to variations in the C-terminal part of the aminoacid sequence of the PGRS ORF translation product.
  • As shown in FIG. 6, an amino acid stretch of 9 residues in length is present in this M. tuberculosis PGRS (ORf reference Rv0746) and is absent from the ORF counterpart of M. bovis BCG, namely the following amino acid sequence:
    NH2-GGAGGAGGSSAGGGGAGGAGGAGGWLLGD-COOH.
  • Furthermore, FIG. 6 shows also that an amino acid stretch of 45 residues in length is absent from this M. tuberculosis PGRS and is present in the ORF counterpart of M. bovis BCG, namely following amino acid sequence:
    NH2-GAGGIGGIGGNANGGAGGNGGTGGQLWGSGGAGVEGGAALSVGDT-
    COOH.
  • Similar observations were made with PPE ORF Rv0442, which showed a 5 codon deletion relative to a M. bovis amino acid sequence.
  • Given that the polymorphism associated with the PE-PGRS or PEE ORFS resulted in extensive antigenic variability or reduced antigen presentation, this would be of immense significance for vaccine design, for understanding protective immunity in tuberculosis and, possibly, explain the varied responses seen in different BCG vaccination programmes.
  • There are several striking parallels between the PGRS proteins and the Epstein-Barr virus-encoded nuclear antigens (EBNA). Both polypeptide families are glycine-rich, contain Gly-Ala repeats that represent more than one third of the molecule, and display variation in the length of the repeat region between different isolates. The Gly-Ala repeat region of EBNA1 has been shown to function as a cis-acting inhibitor of antigen processing and MHC class I-restricted antigen presentation (Levitskaya et al., 1995). The fact that MHC class I knock-out mice are extremely suscepible to M. tuberculosis underlines the importance of MHC class I antigen presentation in protection against tuberculosis. Therefore, it is possible that the PE/PPE protein family also play some role in inhibiting antigen presentation, allowing the bacillus to hide from the host's immune system.
  • As such the novel and nonobvious PGRS polynucleotide from M. bovis which is homolog to the M. tuberculosis ORF Rv0746, and which is contained in the BAC clone No X0175 (See Table 4 for SP6 and T7 endsequences of clone no X0175) of the I-XXXX M. bovis BCG BAC DNA library is part of the present invention, as it represents a starting material in order to define specific probes or primers useful for detection of antigenic variability in mycobacterial strains, possible inhibition of antigen processing as well as to differentiate M. tuberculosis from M. bovis BCG.
  • Thus, a further object of the invention consists in a polynucleotide comprising the following sequence SEQ ID No4:
    CCGACCCAGA CACTGACCGG GCGACCGCTG ATCGGCAACG 60
    GCACCCCCGG GGCGGTCCGC
    AGCGGGGCCA CCGGGGCCCC CGGTGGGTGG CTGCTCGGCG 120
    ACGGCGGGGC CGGCGGGTCC
    GGCGCGGCGG GCTCGGGCGC GCCCGGCGGG GCGGGCGGGG 180
    CTGCCGGGCT GTGGGGTACC
    GGCGGGGCCG GCGGGATCGG CGGAGCCAGC ACCGTACTCG 240
    GCGGCACCGG CGGGGGAGGC
    GGGGTCGGTG GGCTGTGGGG CGCCGGTGGG GCCGGCGGGG 300
    CCGGTGGAAC CGGCCTTGTT
    GGTGGCGACG GCGGGGCCGG TGGGGCCGGC GGGACCGGCG 360
    GACTGCTGGC CGGGCTGATC
    GGTGCCGGCG GAGGTCACGG CGGGACCGGC GGGCTCAGCA 420
    CTAATGGCGA CGGCGGGGTT
    GGCGGGGCCG GCGGGAATGC CGGAATGCTC GCCGGGCCGG 480
    GCGGCGCCGG CGGAGCCGGC
    GGTGACGGCG AAAACCTGGA CACCGGTGGG GACGGCGGGG 540
    CCGGCGGTAG CGCAGGGCTG
    CTGTTCGGCA GCGGCGGCGC CGGCGGCGCC GGCGGATTTG 600
    GTTTCCTCGG TGGGGACGGC
    GGGGCCGGTG GCAACGCCGG GCTGCTGTTG TCCAGCGGCG 660
    GGGCCGGCGG GTTCGGCGGG
    TTCGGCACCG CCGGTGGGGT CGGTGGGGCC GGCGGCAATG 720
    CCGGCTGGCT GGGCTTCGGC
    GGGGCCGGGG GCATCGGCGG AATCGGCGGT AACGCTAACG 780
    GGGGCGCCGG TGGGAACGGC
    GGCACCGGCG GTCAGTTATG GGGTAGCGGC GGCGCCGGCG 840
    TCGAAGGCGG CGCAGCCTTA
    AGCGTCGGCG ACACCGGCGG GGCCGGTGGC GTCGGCGGCA 900
    GCGCCGGGCT GATCGGCACC
    GGCGGCAACG GCGGCAACGG CGGCACCGGC GCCAACGCCG 960
    GCAGCCCCGG AACCGGCGGC
    GCCGGCGGGT TGCTGCTGGG CCAAAACGGG CTCAACGGGT 1020
    TGCCGTAGCC GGGCGGCACG
    GCATGGCTTC CGGGCGTCAA CCACTCGCCG GTGATGCAGA 1080
    TCGGCTGCGG AGCGGGCCGC
    CAAAATGGGG GCCGCCGCGC CAGGTATCTC GGCGAAGATC 1140
    CCCGGCGCTC GAGCGCTTTG
    TCAGAGGCCC GTCGCGGGTC GTCGTGACGA CGGCTATCCG 1200
    GGCGGTGCGG GTTTCGCGGC
    GCGCCCTGTG CCCGGCACCG CCGCCCGTTT GTCGGCAACG 1260
    CCGCCGCGAC CCGTGAGCCG
    TCCAGCAGCT GGCGCCTGCG 1280
  • Polynucleotides of interest have been defined by the inventors as useful detection tools in order to differentiate M. tuberculosis from M. bovis BCG. Such polynucleotides are contained in the 45 aminoacid length coding sequence that is present in M. bovis BCG but absent from M. tuberculosis. This polynucleotide has a sequence beginning (5′end) at the nucleotide at position nt 729 of the sequence SEQ ID N o4 and ending (3′end) at the nucleotide in position nt 863 of the sequence SEQ ID N o4.
  • Thus, part of the present invention is also a polynucleotide which is chosen among the following group of polynucleotides:
  • a) A polynucleotide comprising at least 8 consecutive nucleotides the following nucleotide sequence SEQ ID No5:
    GGGCATCGGC GGAATCGGCG GTAACGCTAA CGGGGGCGCC 60
    GGTGGGAACG GCGGCACCGG
    CGGTCAGTTA TGGGGTAGCG GCGGCGCCGG CGTCGAAGGC 120
    GGCGCAGCCT TAAGCGTCGG
    CGACACC 127
    • b) A polynucleotide which sequence is fully complementary to the sequence of the polynucleotide defined in a);
    • c) A polynucleotide that hybridizes under stringent hybridization conditions with the polynucleotide defined in a) or with the polynucleotide defined in b).
  • The stringent hybridization conditions for the purpose of defining the above disclosed polynucleotide are defined herein before in the specification.
  • The invention also provides for a BAC-based Mycobacterium tuberculosis strain H37Rv genomic DNA library that has been deposited in the Collection Nationale de Cultures de Microorganismes on Nov. 19, 1997 under the accession number I-1945.
  • A further object of the invention consists in a recombinant BAC vector which is choosen among the group consisting of the recombinant BAC vectors belonging to the BAC-based DNA library I-1945.
  • Generally, a recombinant BAC vector of interest may be choosen among the following set or group of BAC vectors contained in the BAC-based DNA library I-1945:
  • Rv101; Rv102; Rv103; Rv104; Rv105; Rv106; Rv107; Rv108; Rv109; Rv10; Rv110; Rv111; Rv112; Rv113; Rv114; Rv115; Rv116; Rv117; Rv118; Rv119; Rv11; Rv120; Rv121; Rv122; Rv123; Rv124; Rv126; Rv127; Rv128; Rv129; Rv130; Rv132; Rv134; Rv135; Rv136; Rv137; Rv138; Rv139; Rv13; Rv140; Rv141; Rv142; Rv143; Rv144; Rv145; Rv146; Rv147; Rv148; Rv149; Rv14; Rv150; Rv151; Rv152; Rv153; Rv154; Rv155; Rv156; Rv157; Rv159; Rv15; Rv160; Rv161; Rv162; Rv163; Rv164; Rv165; Rv166; Rv167; Rv169; Rv16; Rv170; Rv171; Rv172; Rv173; Rv174; Rv175; Rv176; Rv177; Rv178; Rv179; Rv17; Rv180; Rv181; Rv182; Rv183; Rv184; Rv185; Rv186; Rv187; Rv188; Rv18; Rv190; Rv191; Rv192; Rv193; Rv194; Rv195; Rv196; Rv19; Rv1; Rv201; Rv204; Rv205; Rv207; Rv209; Rv20; Rv214; Rv215; Rv217; Rv218; Rv219; Rv21; Rv220; Rv221; Rv222; Rv223; Rv224; Rv225; Rv226; Rv227; Rv228; Rv229; Rv22; Rv230; Rv231; Rv232; Rv233; Rv234; Rv235; Rv237; Rv240; Rv241; Rv243; Rv244; Rv245; Rv246; Rv247; Rv249; Rv24; Rv251; Rv252; Rv253; Rv254; Rv255; Rv257; Rv258; Rv259; Rv25; Rv260; Rv261; Rv262; Rv263; Rv264; Rv265; Rv266; Rv267; Rv268; Rv269; Rv26; Rv270; Rv271; Rv272; Rv273; Rv274; Rv275; Rv276; Rv277; Rv278; Rv279; Rv27; Rv280; Rv281; Rv282; Rv283; Rv284; Rv285; Rv286; Rv287; Rv288; Rv289; Rv28; Rv290; Rv291; Rv292; Rv293; Rv294; Rv295; Rv296; Rv29; Rv2; Rv301; Rv302; Rv303; Rv304; Rv306; Rv307; Rv308; Rv309; Rv30; Rv310; Rv311; Rv312; Rv313; Rv314; Rv315; Rv316; Rv317; Rv318; Rv319; Rv31; Rv32; Rv322; Rv327; Rv328; Rv329; Rv32; Rv330; Rv331; Rv333; Rv334; Rv335; Rv336; Rv337; Rv338; Rv339; Rv33; Rv340; Rv341; Rv343; Rv344; Rv346; Rv347; Rv348; Rv349; Rv34; Rv350; Rv351; Rv352; Rv353; Rv354; Rv355; Rv356; Rv357; Rv358; Rv359; Rv35; Rv360; Rv361; Rv363; Rv364; Rv365; Rv366; Rv367; Rv368; Rv369; Rv36; Rv370; Rv371; Rv373; Rv374; Rv375; Rv376; Rv377; Rv378; Rv379; Rv37; Rv381; Rv382; Rv383; Rv384; Rv385; Rv386; Rv387; Rv388; Rv389; Rv38; Rv390; Rv391; Rv392; Rv393; Rv396; Rv39; Rv3; Rv40; Rv412; Rv413; Rv414; Rv415; Rv416; Rv417; Rv418; Rv419; Rv41; Rv42; Rv43; Rv44; Rv45; Rv46; Rv47; Rv48; Rv49; Rv4; Rv50; Rv51; Rv52; Rv53; Rv54; Rv55; Rv56; Rv57; Rv58; Rv59; Rv5; Rv60; Rv61; Rv62; Rv63; Rv64; Rv65; Rv66; Rv67; Rv68; Rv69; Rv6; Rv70; Rv71; Rv72; Rv73; Rv74; Rv75; Rv76; Rv77; Rv78; Rv79; Rv7; Rv80; Rv81; Rv82; Rv83; Rv84; Rv85; Rv86; Rv87; Rv88; Rv89; Rv8; Rv90; Rv91; Rv92; Rv94; Rv95; Rv96; Rv9.
  • The end sequences of the polynucleotide inserts of each of the above clones corresponding respectively to the sequences adjacent to the T7 promoter and to the Sp6 promoter on the BAC vector are shown in Table 3.
  • It has been shown by the inventors that the minimal overlapping set of BAC vectors of the BAC-based DNA library I-1945 contains 68 unique BAC clones and practically spans almost the whole H37Rv chromosome with the exception of a single gap of approximately 150 kb.
  • More specifically, a recombinant BAC vector of interest is choosen among the following set or group of BAC vectors from the BAC-based DNA library I-1945, the location of which vector DNA inserts on the chromosome of M. tuberculosis is shown in FIG. 3:
  • Rv234; Rv351; Rv166; Rv35; Rv415; Rv404; Rv209; Rv272; Rv30; Rv228; Rv233; Rb38; Rv280; Rv177; Rv48; Rv374; Rv151; Rv238; Rv156; Rv92; Rv3; Rv403; Rv322; Rv243; Rv330; Rv285; Rv233; Rv219; Rv416; Rv67; Rv222; Rv149; Rv279; Rv87; Rv273; Rv266; Rv25; Rv136; Rv414; Rv13; Rv289; Rv60; Rv104; Rv5; Rv165; Rv215; Rv329; Rv240; Rv19; Rv74; Rv411; Rv167; Rv56; Rv80; Rv164; Rv59; Rv313; Rv265; Rv308; Rv220; Rv258; Rv339; Rv121; Rv419; Rv418; Rv45; Rv217; Rv134; Rv17; Rv103; Rv21; Rv22; Rv2; Rv270; Rv267; Rv174; Rv257; Rv44; Rv71; Rv7; Rv27; Rv191; Rv230; Rv128; Rv407; Rv106; Rv39; Rv255; Rv74; Rv355; Rv268; Rv58; Rv173; Rv264; Rv417; Rv401; Rv144; Rv302; Rv81; Rv163; Rv281; Rv221; Rv420; Rv175; Rv86; Rv412; Rv73; Rv269; Rv214; Rv287; Rv42; Rv143.
  • The polynucleotides disclosed in Table 3 may be used as probes in order to select a given clone of the BAC DNA library I-1945 for further use.
  • The invention also provides for a BAC-based Mycobacterium bovis strain Pasteur genomic DNA library that has been deposited in the Collection Nationale de Cultures de Microorganismes on XXXX XX, 1998 under the accession number I-XXXX.
  • A further object of the invention consists in a recombinant BAC vector which is chosen among the group consisting of the recombinant BAC vectors belonging to the BAC-based DNA library I-XXXX. This DNA library contains approximately 1600 clones. The average insert size is estimated to be ˜80 kb.
  • Generally, a recombinant BAC vector of interest may be chosen among the following set or group of BAC vectors contained in the BAC-based DNA library I-XXXX:
  • X0001; X0002; X0003; X0004; X0006; X0007; X0008; X0009; X0010; X0012; X0013; X0014; X0015; X0016; X0017; X0018; X0019; X0020; X0021; X0175.
  • The end sequences of the polynucleotide inserts of each of the above clones corresponding respectively to the sequences adjacent to the T7 promoter and to the Sp6 promoter on the BAC vector are shown in Table 4.
  • The polynucleotides disclosed in Table 4 may be used as probes in order to select a given clone of the BAC DNA library I-XXXX for further use.
  • Are also part of the invention the polynucleotide inserts that are contained in the above described BAC vectors, that are useful as primers or probes.
  • These polynucleotides and nucleic acid fragments may be used as primers for use in amplification reactions, or as nucleic probes.
  • PCR is described in the U.S. Pat. No. 4,683,202. The amplified fragments may be identified by an agarose or a polyacrylamide gel electrophoresis, or by a capillary electrophoresis or alternatively by a chromatography technique (gel filtration, hydrophobic chromatography or ion exchange chromatography). The specificity of the amplification may be ensured by a molecular hybridization using, for example, one of the initial primers as nucleic probes.
  • Amplified nucleotide fragments are used as probes in hybridization reactions in order to detect the presence of one polynucleotide according to the present invention or in order to detect mutations in the genome of the given mycobacterium of interest, specifically a mycobacterium belonging to the Mycobacterium tuberculosis complex and more specifically Mycobacterium tuberculosis and Mycobacterium bovis BCG.
  • Are also part of the present invention the amplified nucleic fragments (
    Figure US20060063182A1-20060323-P00900
    amplicons
    Figure US20060063182A1-20060323-P00901
    ) defined herein above.
  • These probes and amplicons may be radioactively or non-radioactively labeled, using for example enzymes or fluorescent compounds.
  • Other techniques related to nucleic acid amplification may also be used and are generally preferred to the PCR technique.
  • The Strand Displacement Amplification (SDA) technique (Walker et al., 1992) is an isothermal amplification technique based on the ability of a restriction enzyme to cleave one of the strands at his recogntion site (which is under a hemiphosphorothioate form) and on the property of a DNA polymerase to initiate the synthesis of a new strand from the 3′OH end generated by the restriction enzyme and on the property of this DNA polymerase to displace the previously synthesized strand being localized downstream. The SDA method comprises two main steps:
    • a) The synthesis, in the presence of dCTP-alpha-S, of DNA molecules that are flanked by the restriction sites that may be cleaved by an appropriate enzyme.
    • b) The exponential amplification of these DNA molecules modified as such, by ezyme cleavage, strand displacement and copying of the displaced strands. The steps of cleavage, strand displacement and copy are repeated a sufficient number of times in order to obtain an accurate sensitivity of the assay.
  • The SDA technique was initially realized using the restriction endonuclease HincII but is now generally practised with an endonuclease from Bacillus stearothermophilus (BSOBI) and a fragment of a DNA polymerase which is devoid of any 5′→3′exonuclease activity isolated from Bacilllus cladotenax (exo-Bca) [=exo-minus-Bca]. Both enzymes are able to operate at 60° C. and the system is now optimized in order to allow the use of dUTP and the decontamination by UDG. When unsing this technique, as described by Spargo et al. in 1996, the doubling time of the target DNA is of 26 seconds and the amplification rate is of 1010 after an incubation time of 15 min at 60° C.
  • The SDA amplification technique is more easy to perform than PCR (a single thermostated waterbath device is necessary) and is faster thant the other amplification methods.
  • Thus, another object of the present invention consists in using the nucleic acid fragments according to the invention (primers) in a method of DNA or RNA amplification according to the SDA technique. For performing SDA, two pairs of primers are used: a pair of external primers (B1, B2) consisting of a sequence specific for the target polynucleotide of interest and a pair of internal primers (S1, S2) consisting of a fusion oligonucleotide carrying a site that is recognized by a restriction endonuclease, for exemple the enzyme BSOBI.
  • The operating conditions to perform SDA with such primers are described in Spargo et al. 1996.
  • The polynucleotides of the invention and their above described fragments, especially the primers according to the invention, are useful as technical means for performing different target nucleic acid amplification methods such as:
      • TAS (Transcription-based Amplification System), described by Kwoh et al. in 1989;
      • SR (Self-Sustained Sequence Replication), described by Guatelli et al. in 1990.
      • NASBA (Nucleic acid Sequence Based Amplification), described by Kievitis et al. in 1991.
      • TMA (Transcription Mediated Amplification).
  • The polynucleotides according to the invention are also useful as technical means for performing methods for amplification or modification of a nucleic acid used as a probe, such as:
      • LCR (Ligase Chain Reaction), described by Landegren et al. in 1988 and improved by Barany et al. in 1991 who employ a thermostable ligase.
      • RCR (Repair Chain Reaction) described by Segev et al. in 1992.
      • CPR (Cycling Probe Reaction), described by Duck et al. in 1990.
      • Q-beta replicase reaction, described by Miele et al. in 1983 and improved by Chu et al. in 1986, Lizardi et al. in 1988 and by Burg et al. and Stone et al. in 1996.
  • When the target polynucleotide to be detected is a RNA, for example a mRNA, a reverse transcriptase enzyme will be used before the amplification reaction in order to obtain a cDNA from the RNA contained in the biological sample. The generated cDNA is subsequently used as the nucleic acid target for the primers or the probes used in an amplification process or a detection process according to the present invention.
  • The non-labeled polynucleotides or oligonucleotides of the invention may be directly used as probes. Nevertheless, the polynucleotides or oligonucleotides are generally labeled with a radioactive element (32P, 35S, 3H, 125I) or by a non-isotopic molecule (for example, biotin, acetylaminofluorene, digoxigenin, 5-bromodesoxyuridin, fluorescein) in order to generate probes that are useful for numerous applications.
  • Examples of non-radioactive labeling of nucleic acid fragments are described in the french patent No FR-7810975 or by Urdea et al. or Sanchez-Pescador et al., 1988.
  • In the latter case, other labeling techniques may be also used such as those described in the french patents FR-2,422,956 and 2,518,755. The hybridization step may be performed in diffrent ways (Matthews et al., 1988). The more general method consists of immobilizing the nucleic acid that has been extracted from the biological sample onto a substrate (nitrocellulose, nylon, polystyrene) and then to incubate, in defined conditions, the target nucleic acid with the probe. Subsequently to the hybridization step, the excess amount of the specific probe is discarded and the hybrid molecules formed are detected by an appropriate method (radioactivity, fluorescence or enzyme activity measurement).
  • Advantageously, the probes according to the present invention may have structural characteristics such that they allow the signal amplification, such structural characteristics being, for example, branched DNA probes as those described by Urdea et al. in 1991 or in the European patent No EP-0225,807 (Chiron). In another advantageous embodiment of the probes according to the present invention, the latters may be used as
    Figure US20060063182A1-20060323-P00900
    capture probes
    Figure US20060063182A1-20060323-P00901
    , and are for this purpose immobilized on a substrate in order to capture the target nucleic acid contained in a biological sample. The captured target nucleic acid is subsequently detected with a second probe which recognizes a sequence of the target nucleic acid which is different from the sequence recognized by the capture probe.
  • The oligonucleotide probes according to the present invention may also be used in a detection device comprising a matrix library of probes immobilized on a substrate, the sequence of each probe of a given length being localized in a shift of one or several bases, one from the other, each probe of the matrix library thus being complementary to a distinct sequence of the target nucleic acid. Optionally, the substrate of the matrix may be a material able to act as an electron donor, the detection of the matrix poisitons in which an hybridization has occurred being subsequently determined by an electronic device. Such matrix libraries of probes and methods of specific detection of a targer nucleic acid is described in the European patent application No EP-0713,016 (Affymax technologies) and also in the U.S. Pat. No. 5,202,231 (Drmanac).
  • Since almost the whole length of a mycobacterial chromososme is covered by a BAC-based genomic DNA libraries according to the present invention (i.e. 97% of the M. tuberculosis chromosome is covered by the BAC library I-1945), these DNA libraries will play an important role in a plurality of post-genomic applications, such as in mycobacterial gene expression studies where the canonical set of BACs could be used as a matrix for hybridization studies. Probing such matrices with cDNA probes prepared from total mRNA will uncover genetic loci induced or repressed under different physiological conditions (Chuang et al., 1993; Trieselmann et al., 1992). As such, the H37Rv BAC library represents a fundamental resource for present and future genomics investigations.
  • The BAC vectors or the polynucleotide inserts contained therein may be directly used as probes, for example when immobilized on a substrate such as described herein before.
  • The BAC vectors or their polynucleotide inserts may be directly asdorbed on a nitrocellulose membrane, at predetermined locations on which one or several polynucleotides to be tested are then put to hybridize therewith.
  • Preferably, a collection of BAC vectors that spans the whole genome of the mycobacterium under testing will be immobilized, such as, for example, the set of 68 BAC vectors of the I-1945 DNA library that is described elsewhere in the specification and shown in FIG. 3.
  • The immobilization and hybridization steps may be performed as described in the present Materials and Methods Section.
  • As another illustrative embodiment of the use of the BAC vectors of the invention as polynucleotide probes, these vectors may be useful to perform a transcriptional activity analysis of mycobacteria growing in different environmental conditions, for example under conditions in which a stress response is expected, as it is the case at an elevated temperature, for example 40° C.
  • In this specific embodiment of the invention, Genescreen membranes may be used to immobilize the restriction endonuclease digests (HindIII digests for the BAC DNA library I-1945) of the BAC vectors by tranfer from a gel (Trieselmann et al., 1992).
  • Alternatively, the BAC vectors may be immobilized for dot blot experiments as follows. First, the DNA concentration of each BAC clone is determined by hybridization of blots of clone DNAs and of a BAC vector concentration standard with a BAC vector specific DNA probe. Hybridization is quantified by the Betascope 603 blot analyzer (Betagen Corp.), which colects beta particles directly from the blot with high efficiency. Then, 0.5 μg of each clone DNA is incubated in 0.25 M NaOH and 10 mM EDTA at 65° C. for 60 min to denature the DNA and degrade residual RNA contaminants. By using a manifold filtration system (21 by 21 wells), each clone DNA is blotted onto a GeneScreen Plus nylon membrane in the alkaline solution. After neutralization, the blots are baked at 85° C. for 2 h under vacuum. Positive and negative controls are added when necessary. In order to perform this procedure, it may be referred to the article of Chuang et al. (1993).
  • For RNA extractions, cells grown in a suitable volume of culture medium may, for example, be immediately mixed with an equal volume of crushed ice at −70° C. and spun at 4° C. in a 50 ml centrifugation tube. The cell pellet is then suspended in 0.6 ml of ice-cold buffer (10 mM KCl, 5 mM MgCl, 10 mM Tris; pH 7.4) and then immediately added to 0.6 ml of hot lysis buffer (0.4 M NaCl, 40 mM EDTA, 1% beta-mercaptoethanol 1% SDS, 20 mM Tris; pH 7.4) containing 100 μl of water saturated phenol. This mixture is incubated in a boiling water bath for 40 s. The debris are removed by centrifugation. The supernatant is extracted with phenol-chloroform five times, ethanol precipitated, and dried. The dried RNA pellet is dissolved in water before use.
  • Then labeled total cDNA may be prepared by the following method. The reaction mixture contains 15 μg of the previously prepared total RNA, 5 μg of pd(N6) (random hexamers from Pharmacia Inc.), 0.5 mM dATP, 0.5 mM dGTP and 0.5 mM DTTP, 5 μM dCTP, 100 μCi of [α-32P]dCTP (3,000 Ci/mmol), 50 mM Tris-HCl (pH 8.3), 6 mM MgCl2, 40 mM Kcl, 0.5 U of avian myeloblastosis virus reverse transcriptase (Life Science Inc.) in a total volume of 50 μl. The reaction is allowed to continue overnight at room temperature. EDTA and NaOH are then added to final concentrations of 50 mM and 0.25 M, respectively, and the mixture is incubated at 65° C. for 30 min to degrade the RNA templates. The cDNA is then ready to use after neutralization by adding Hcl and Tris buffer.
  • The hybridization step may be performed as described by Chuang et al. (1993) and briefly disclosed hereinafter. The DNA dot blot is hybridized to 32P-labeled total cDNA in a solution containing 0.1% polyvinylpyrrolidone, 0.1% Ficoll, 0.1% sodium Ppi, 0.1% bovine serum albumin, 0.5% SDS, 100 mM NaCl, and 0.1 mM sodium citrate, pH 7.2, at 65° C. for 2 days and then washed with a solution containing 0.1% SDS, 100 mM NaCl, and 10 mM Na-citrate, pH 7.2. The same dot blot is used for hybridization with both control and experimental cDNAs, with an alkaline probe stripping procedure (soaked twice in 0.25M NaOH-0.75 M NaCl at room temperature, 30 min each, neutralized, and completely dried at 65° C. for at least 30 min) between the two hybridizations. Quantification may be done with the Betascope 603 blot analyzer (Betagen Corp.).
  • As it flows from the above technical teachings, another object of the invention consists in a method for detecting the presence of mycobateria in a biological sample comprising the steps of:
    • a) bringing into contact the recombinant BAC vector or a purified polynucleotide according to the invention with a biological sample.
    • b) detecting the hybrid nucleic acid molecule formed between said purified polynucleotide and the nucleic acid molecules contained within the biological sample.
  • The invention further deals with a method for detecting the presence of mycobacteria in a biological sample comprising the steps of:
    • a) Bringing into contact the recombinant BAC vector or a purified polynucleotide according to the invention that has been immobilized onto a substrate with a biological sample.
    • b) Bringing into contact the hybrid nucleic acid molecule formed between said purified polynucleotide and the nucleic acid contained in the biological sample with a labeled recombinant BAC vector or a polynucleotide according to the invention, provided that said polynucleotide and polynucleotide of step a) have non-overlapping sequences.
  • Another object of the invention consists in a method for detecting the presence of mycobacteria in a biological sample comprising the steps of:
    • a) Bringing into contact the nucleic acid molecules contained in the biological sample with a pair of primers according to the invention.
    • b) Amplifying said nucleic acid molecules;
    • d) detecting the nucleic acid fragments that have been amplified, for example by gel electrophoresis or with a labeled polynucleotide according to the invention.
  • In one specific embodiment of the above detection and/or amplification methods, said methods comprise an additional step wherein before step a), the nucleic acid molecules of the biological sample have been made available to a hybridization reaction.
  • In another specific embodiment of the above detection methods, said methods comprise an additional step, wherein, before the detection step, the nucleic acid molecules that are not hybridized with the immobilized purified polynucleotide are removed.
  • Also part of the invention is a kit for detecting mycobacteria in a biological sample comprising:
    • a) A recombinant BAC vector or a purified polynucleotide according to the invention;
    • b) Reagents necessary to perform a nucleic acid hybridization reaction.
  • The invention also pertains to a kit for detecting a mycobacteria in a biological sample comprising:
    • a) A recombinant BAC vector or a purified polynucleotide according to the invention that is immobilized onto a substrate.
    • b) Reagents necessary to perform a nucleic acid hybridization reaction.
    • c) A purified polynucleotide according to the invention which is radioactively or non-radioactively labeled, provided that said polynucleotide and the polynucleotide of step a) have non-overlapping sequences.
  • Moreover, the invention provides for a kit for detecting mycobacteria in a biological sample comprising:
    • a) A pair of purified primers according to the invention;
    • b) Reagents necessary to perform a nucleic acid amplification reaction;
    • c) Optionally, a purified polynucleotide according to the invention useful as a probe.
  • The invention embraces also a method for detecting the presence of a genomic DNA, a cDNA or a mRNA of mycobacteria in a biological sample, comprising the steps of:
    • a) Bringing into contact the biological sample with a plurality of BAC vectors according to the invention or purified polynucleotides according to the invention, that are immobilized on a substrate;
    • b) Detecting the hybrid complexes formed.
  • The invention also provides a kit for detecting the presence of genomic DNA, cDNA or mRNA of a mycobacterium in a biological sample, comprising:
    • a) A substrate on which a plurality of BAC vectors according to the invention or purified polynucleotides according to the invention have been immobilized;
    • b) Optionally, the reagents necessary to perform the hybridization reaction.
  • Additionally, the recombinant BAC vectors according to the invention and the polynucleotide inserts contained therein may be used for performing detection methods based on
    Figure US20060063182A1-20060323-P00900
    molecular combing
    Figure US20060063182A1-20060323-P00901
    . Said methods consist in methods for aligning macromolecules, especially DNA and are applied to processes for detecting, for measuring intramolecular distance, for separating and/or for assaying a macromolecule, especially DNA in a sample.
  • These
    Figure US20060063182A1-20060323-P00900
    molecular combing
    Figure US20060063182A1-20060323-P00901
    methods are simple methods, where the triple line S/A/B (meniscus) resulting form the contact between a solvent A and the surface S and a medium B is caused to move on the said surface S, the said macromolecules (i.e. DNA) having a part, especially an end, anchored on the surface S, the other part, especially the other end, being in solution in the solvent A. These methods are particularly fully described in the PCT Application No. PCT/FR 95/00165 files on Feb. 11, 1994 (Bensimon et al.).
  • When performing the
    Figure US20060063182A1-20060323-P00900
    molecular combing
    Figure US20060063182A1-20060323-P00901
    method with the recombinant BAC vectors according to the inventions or their polynucleotide inserts, the latters may be immobilized (
    Figure US20060063182A1-20060323-P00900
    anchored
    Figure US20060063182A1-20060323-P00901
    ) on a suitable substrate and aligned as described in the PCT Application No. PCT/FR 95/00165, the whole teachings of this PCT Application being herien incorporated by reference. Then, polynucleotides to be tested, preferably under the form of radioactively or non radioactively labeled polynucleotides, that may consist of fragments of genomic DNA, cDNA etc. are brought into contact with the previously aligned polynucleotides according to the present invention and then their hybridization position on the aligned DNA molecules is determined using any suitable means including a microscope or a suitable camera device.
  • Thus, the present invention is also directed to a method for the detection of the presence of a polynucleotide of mycobacterial origin in a biological sample and/or for physical mapping of a polynucleotide on a genomic DNA, said method comprising:
    • a) Aligning at least one polynucleotide contained in a recombinant BAC vector according to the invention on the surface of a substrate;
    • b) Bringing into contact at least one polynucleotide to be tested with the substrate on which the at least one polynucleotide of step a) has been aligned;
    • c) detecting the presence and/or the location of the tested polynucleotide on the at least one aligned polynucleotide of step a).
  • The invention finally provides for a kit for performing the above method, comprising:
    • a) a substrate whose surface has at least one polynucleotide contained in a recombinant BAC vector according to the invention;
    • b) optionally, reagents necessary for labeling DNA;
    • c) optionnally, reagents necessary for performing a hybridization reaction.
  • In conclusion, it may be underlined that the alliance of such BAC-based approaches such as described in the present specification to the advances in comparative genomics by the availability of an increased number of complete genomes, and the rapid increase of well-characterized gene products in the public databases, will allow the one skilled in the art an exhaustive analysis of the mycobacterial genome.
  • Materials and Methods
  • 1. DNA-preparation. Preparation of M. tuberculosis H37Rv DNA in agarose plugs was conducted as previously described (Canard et al., 1989; Philipp et al., 1996b). Plugs were stored in 0.2 M EDTA at 4° C. and washed 3 times in 0.1% Triton X-100 buffer prior to use.
  • 2. BAC vector preparation. pBeloBAC11 was kindly provided by Dr. Shizuya, Department of Biology, California Institute of Technology (Pasadena, Calif.). The preparation followed the description of Woo et al., 1994 (Woo et al., 1994).
  • 3. Partial digestion with HindIII. Partial digestion was carried out on plugs, each containing approximately 10 μg of high molecular weight DNA, after three one hour equilibration steps in 50 ml of HindIII 1× digestion buffer (Boehringer Mannheim, Mannheim, Germany) plus 0.1% Triton X-100. The buffer was then removed and replaced by 1 ml/plug of ice-cold HindIII enzyme buffer containing 20 units of HindIII (Boehringer). After two hours incubation on ice, the plugs were transferred to a 37° C. water bath for 30 minutes. Digestions were stopped by adding 500 μl of 50 mM EDTA (pH 8.0).
  • 4. Size selection. The partially digested DNA was subjected to contour-clamped homogenous electric field (CHEF) electrophoresis on a 1% agarose gel using a BioRad DR III apparatus (BioRad, Hercules, Calif.) in 1×TAE buffer at 13° C., with a ramp from 3 to 15 seconds at 6 V/cm for 16 hours. Agarose slices from 25 to 75 kb, 75 to 120 kb and 120 to 180 kb were excised from the gel and stored in TE at 4° C.
  • 5. Ligation and transformation. Agarose-slices containing fractions from 25 to 75 kb, 75 to 120 kb and 120 to 180 kb were melted at 65° C. for 10 minutes and digested with Gelase (Epicentre Technologies, Madison, Wis.), using 1 unit per 100 μl gel-slice. 25-100 ng of the size-selected DNA was then ligated to 10 ng of HindIII digested, dephosphorylated pBeloBAC11 in a 1:10 molar ratio using 10 units of T4 DNA ligase (New England Biolabs, Beverly, Mass.) at 16° C. for 20 hours. Ligation mixtures were heated at 65° C. for 15 minutes, then drop-dialysed against TE using Millipore VS 0.025 mM membranes (Millipore, Bedford, Mass.). Fresh electrocompetent E. coli DH10B cells (Sheng et al., 1995) were harvested from 200 ml of a mid-log (OD550=0.5) culture grown in SOB medium. Cells were washed three times in ice-cold water, and finally resuspended in ice-cold water to a cell density of 1011 cells/ml (OD550=150). 1 μl of the ligation-mix was used for electroporation of 30 μl of electrocompetent DH10B E. coli using a Eurogentec Easyject Plus electroporator (Eurogentec, Seraing, Belgium), with settings of 2.5 kV, 25 μF, and 99½, in 2 mm wide electroporation cuvettes. After electroporation, cells were resuspended in 600 μl of SOC medium, allowed to recover for 45 minutes at 37° C. with gentle shaking, and then plated on LB agar containing 12.5 μg/ml chloramphenicol (CM), 50 μg/ml X-gal, and 25 μg/ml IPTG. The plates were incubated overnight and recombinants (white colonies) were picked manually to 96 well plates. Each clone was inoculated 3 times (2×200 μl and 1×100 μl of 2YT/12.5 μg/ml CM per clone) and incubated overnight. One of the microtiter plates, containing 100 μl culture per well, was maintained as a master plate at −80° C. after 100 ml of 80% glycerol were added to each well while minipreps (Sambrook et al., 1989) were prepared from the remaining two plates to check for the presence of inserts. Clones containing inserts were then designated “Rv” clones, repicked from the master plate to a second set of plates for storage of the library at −80° C.
  • 6. Preparation of DNA for sizing, direct sequencing and comparative genomics. A modified Birnboim and Doly protocol (Birnboim et al., 1979) was used for extraction of plasmid DNA for sequencing purposes. Each Rv clone was inoculated into a 50 ml Falcon polypropylene tube containing 40 ml of 2YT medium with 12.5 μg/ml of CM and grown overnight at 37° C. with shaking. Cells were harvested by centrifugation and stored at −20° C. The frozen pellet was resuspended in 4 ml of Solution A (50 mM glucose, 10 mM EDTA, 25 mM Tris, pH 8.0) and 4 ml of freshly prepared solution B (0.2 M NaOH, 0.2% SDS) was then added. The solution was gently mixed and kept at room temperature for 5 minutes before adding 4 ml of ice-cold solution C (3M Sodium Acetate, pH 4.7). Tubes were kept on ice for 15 min, and centrifuged at 10,000 rpm for 15 min. After isopropanol precipitation, the DNA pellet was dissolved in 600 μl RNase solution (15 mM Tris HCl pH 8.0, 10 μg/ml RNase A). After 30 minutes at 37° C. the DNA solution was extracted with chloroform:isoamylalcohol (24:1) and precipitated from the aqueous phase using isopropanol. The DNA pellet was then rinsed with 70% ethanol air-dried and dissolved in 30 μl distilled water. In general, DNA prepared by this method was clean and concentrated enough to give good quality results by automatic sequencing (at least 300 bp of sequence). For a few DNA preparations, an additional polyethylene glycol (PEG) precipitation step was necessary, which was performed as follows. The 30 μl of DNA solution were diluted to 64 μl, mixed gently and precipitated using 16 μl 4M NaCl and 80 μl of 13% PEG 8000. After 30 min on ice the tubes were centrifuged at 4° C., the pellet carefully rinsed with 70% ethanol, air-dried and diluted in 20 μl of distilled water.
  • 7. Sizing of inserts. Insert sizes were determined by pulsed-field gel electrophoresis (PFGE) after cleavage with DraI (Promega). 100-200 ng of DNA was DraI-cleaved in 20 μl total reaction volume, following the manufacturer's recommendations, then loaded onto a 1% agarose gel and migrated using a pulse of 4 s for 15 h at 6.25 V/cm at 10° C. on an LKB-Pharmacia CHEF apparatus. Mid-range and low-range PFGE markers (New England Biolabs) were used as size standards. Insert sizes were estimated after ethidium bromide staining of gels.
  • 8. Direct sequencing. For each sequencing reaction 7 μl BAC DNA (300-500 ng), 2 μl primer (2 μM), 8 μl reaction mix of the Taq DyeDeoxy Terminator cycle sequencing kit (Applied Biosystems) and 3 μl distilled water were used. After 26 cycles (96° C. for 30 sec; 56° C. for 15 sec; 60° C. for 4 min) in a thermocycler (MJ-research Inc., Watertown, Mass.) DNA was precipitated using 70 μl of 70% ethanol/0.5 mM MgCl2, centrifuged, rinsed with 70% ethanol, dried and dissolved in 2 μl of formamide/EDTA buffer. SP6 and T7 samples of 32 BAC clones were loaded onto 64 lane, 6% polyacrylamide gels and electrophoresis was performed on a Model 373A automatic DNA sequencer (Applied Biosystems) for 12 to 16 hours. The sequences of oligonucleotides used as primers are shown in Table 1.
  • 9. DOP-PCR. As an alternate procedure we used partially degenerate oligonucleotides in combination with vector-specific (SP6 or T7) primers to amplify insert ends of BAC clones, following a previously published protocol for P1 clones (Liu et al., 1995). The degenerate primers Deg2, Deg3, Deg4, Deg6 (Table 1) gave the best results for selected amplification of insert termini.
    TABLE 1
    Primers used for PCRs and sequencing
    Vector specific Primers for DOP PCR- first
    amplification step:
    SP6-BAC1: AGT TAG CTC ACT CAT TAG GCA
    T7-BAC1: GGA TGT GCT GCA AGG CGA TTA
    Vector specific Primers (direct sequencing,
    nested primer for second PCR step)
    SP6 Mid: AAA CAG CTA TGA CCA TGA TTA CGC CAA
    T7-Belo2: TCC TCT AGA GTC GAC CTG CAG GCA
    Degenerate Primers:
    Deg2: TCT AGA NNN NNN TCC GGC
    Deg3: TCT AGA NNN NNN GGG CCC
    Deg4: CGT TTA AAN NNN NWA GGC CG
    Deg6: GGT ACT AGT NNN NNW TCC GGC
    Primers used for the amplification of M. bovis
    DNA in polymorphic chromosomal region of Rv58:
    Primer 1: ACG ACC TCA TAT TCC GAA TCC C
    Primer 2: GCA TCT GTT GAG TAC GCA CTT CC
  • 10. Screening by pooled PCR. To identify particular clones in the library which could not be detected by random end-sequencing of the 400 BAC clones, PCR-screening of DNA pools was performed. Primers were designed for regions of the chromosome where no BAC coverage was appearent using cosmid-or H37Rv whole genome shotgun sequences. Primers were designed to amplify approximately 400-500 bp. Ninety-six-well plates containing 200 μl 2YT/12.5 μg/ml CM per well were inoculated with 5 μl of −80° C. glycerol stock cultures each from the master plates and incubated overnight. The 96 clones of each plate were pooled by taking 20 μl of culture from each well and this procedure was repeated for 31 plates. Pooled cultures were centrifuged, the pellets were resuspended in sterile water, boiled for 5 minutes, centrifuged and the supernatants kept for PCRs. As an initial screening step, the 31 pools of a total of 2976 BACs, representing about two thirds of the library were tested for the presence of a specific clone using appropriate PCR primers. PCR was performed using 10 μl of supernatant, 5 μl of assay buffer (100 mM b-mercaptoethanol, 600 mM Tris HCl (pH 8.8), 20 mM MgCl2, 170 mM (NH4)2SO4), 5 μl of Dimethylsulfoxide (DMSO), 5 μl of dNTPs (20 mM), 5 μl of water, 10 μl primer (2 μM), 10 μl inverse primer (2 μM) and 0.2 units of Taq DNA polymerase (Boehringer). 32 cycles of PCR (95° C. for 30 s, 55° C. for 1 min 30 s, 72° C. for 2 min) were performed after an initial denaturation at 95° C. for 1 min. An extension step at 72° C. for 5 min finished the PCR-If a pool of 96 clones yielded an appropriate PCR product (FIG. 1A), subpools were made to identify the specific clone. Subpools representative for lane A of a 96 well plate were made by pooling clones 1 to 12 from lane A into a separate tube. Subpools for lanes B to H were made in the same way. In addition, subpools of each of the 12 rows (containing 8 clones each) were made, so that for one 96 well plate, 20 subpools were obtained. PCR with these 20 subpools identified the specific clone (FIG. 1B, lower gel portion). If more than one specific clone was present among the 96 clones of one plate (FIG. 1B, upper gel portion), additional PCR reactions had to be performed with the possible candidates (data not shown).
  • 11. Genomic comparisons. DNA from the BAC clone Rv58 was digested with the restriction endonucleases EcoRI and PvuII, and resolved by agarose gel electrophoresis at low voltage overnight (1.5 V/cm). DNA was transferred via the method of Southern to nitrocellulose membranes (Hybond C extra, Amersham) following standard protocols (Sambrook et al., 1989), then fixed to the membranes at 80° C. for 2 hours. The blot was hybridized with 32P labelled total genomic DNA from M. tuberculosis H37Rv, M. bovis type strain (ATCC 19210) or M. bovis BCG Pasteur. Hybridization was performed at 37° C. overnight in 50% formamide hybridization buffer as previously described (Philipp et al., 1996b). Results were interpreted from the autoradiograms.
  • 12. Computer analysis. Sequence data from the automated sequencer ABI373A were transferred as binary data to a Digital Alpha 200 station or Sun SparcII station and analysed using TED, a sequence analysis program from the Staden software package (Dear et al., 1991). Proof-read sequences were compared using the BLAST programs (Altschul et al., 1990) to the M. tuberculosis H37Rv sequence databases of the Sanger Centre, containing the collected cosmid sequences (TB.dbs) and whole-genome shotgun reads (TB_shotgun_all.dbs) (http://www.sanger.ac.uk/). In addition, local databases containing 1520 cosmid endsequences and the accumulating BAC endsequences were used to determine the exact location of end-sequenced BACs on the physical and genetic map. MycDB (Bergh et al., 1994) and public databases (EMBL, Genbank) were also used to compare new sequences, but to a lesser extent. The organization of the open reading frames (ORFs) in the polymorphic region of clone Rv58 was determined using the DIANA software established at the Sanger Centre.
    • EXAMPLES Example 1 Construction of a pBeloBAC11 Library of M. tuberculosis H37Rv
  • Partial HindIII fragments of H37Rv DNA in the size range of 25 to 180 kb were ligated into pBeloBAC11 and electroporated into strain E. coli DH10B. While cloning of fractions I (25 to 75 kb) and 11 (75 to 120 kb)-gave approximately 4×104 transformants (white colonies), cloning of fraction m (120 to 180 kb) repeatedly resulted in empty clones. Parallel cloning experiments using partial HindIII digests of human DNA resulted in stable inserts for all three fractions (data not shown), suggesting that the maximum size of large inserts in BAC clones is strongly dependent on the source of the DNA. Analysis of the clones for the presence of inserts revealed that 70% of the clones had an insert of the appropriate size while the remaining 30% of white colonies represented empty or lacZ′-mutated clones. Size determination of randomly selected, DraI-cleaved BACs via PFGE showed that the insert sizes ranged for the majority of the clones between 40 kb and 100 kb with an average size of 70 kb. Clones with inserts of appropriate size were designated with “Rv” numbers, recultured and stored at −80° C. for further use.
    • Example 2 Direct DNA Sequence Analysis of BACs
  • To characterize the BAC clones, they were systematically subjected to insert termini sequencing. Two approaches, direct sequencing of BAC DNA and PCR with degenerate oligonucleotide primers (DOP), adapted to the high G+C content of mycobacterial DNA, were used. In a first screening phase, 50 BAC clones designated Rv1 to Rv50 were analysed using both methods in parallel. Except for two clones, where the sequences diverged significantly, the sequences obtained by the two methods only differed in length. Sequences obtained directly were on average about 350 bp long and for 95% of the clones both the SP6 and T7 endsequences were obtained at the first attempt. Sequences obtained by DOP-PCR were mostly shorter than 300 bp. For 40% of the BACs we obtained only very short amplicons of 50 to 100 base pairs from one end. In two cases the sequence obtained with the DOP-PCR differed from the sequences obtained by direct sequencing, and in these cases E. coli or vector sequences were amplified (data not shown). Taking the advantages and disadvantages of both methods into account, we decided to use direct termini sequencing for the systematic determination of the SP6 and T7 end-sequences.
    • Example 3 Representativity of the Library
  • After having determined the end-sequences of 400 BACs a certain redundancy was seen; The majority of clones were represented at least 3 to 4 times. Maximum redundancy was seen in the vicinity of the unique rrn operon, as 2.5% of the clones carried identical fragments that bridge the cosmids Y50 and Y130 (FIG. 3, approximate position at 1440 kb). The majority of clones with identical inserts appeared as two variants, corresponding to both possible orientations of the HindIII fragment in pBeloBAC11. This suggests that the redundancy was not the result of amplification during library construction, but due to the limited number of possible combinations of partial HindIII fragments in the given size-range of 25 to 120 kb. To detect rare BAC clones, a pooled PCR protocol was used. Primers were designed on the basis of the existing cosmid sequences and used to screen 31 pools of 96 BAC clones. When positive PCR products of the correct size were obtained, smaller subpools (of 8 or 12 clones each) of the corresponding pool were subsequently used to identify the corresponding clone (FIG. 1). With this approach 20 additional BACs (Rv401-Rv420) were found for the regions where no BACs were found with the initial systematic sequencing approach. The endsequences of these BACs (Rv401-420) were determined by direct sequencing, which confirmed the predicted location of the clones on the chromosome. A 97% coverage of the genome of H37Rv with BAC clones was obtained. Only one region of ˜150 kb was apparently not represented in the BAC library as screening of all pools with several sets of specific primers did not reveal the corresponding clone. This was probably due to the fact that HindIII fragments of mycobacterial DNA larger than 110 kb are very difficult to establish in E. coli and that a HindIII fragment of ˜120 kb is present in this region of the chromosome (data not shown).
    • Example 4 Establishing a BAC Map
  • Using all endsequence and shotgun-sequence data from the H37Rv genome sequencing project, most of the BAC clones could then be localized by sequence comparison on the integrated map of the chromosome of M. tuberculosis strain H37Rv (Philipp et al., 1996b) and an ordered physical map of the BAC-clones was established. PCR with primers from the termini sequences of selected BACs were used for chromosomal walking and confirmation of overlapping BACs (data not shown). The correct order of BACs on the map was also confirmed more recently, using 40,000 whole genome shotgun reads established at the Sanger Centre. In addition, pulsed-field gel electrophoresis of DraI digests of selected BACs was performed (FIG. 2) in order to see if the approximate fragment size and the presence or absence of DraI cleavage sites in the insert were consistent with the location of the BACs on the physical map (FIG. 3). Comparison of the sequence-based BAC-map with the physical and genetic map, established by PFGE and hybridization experiments (Philipp et al., 1996b), showed that the two maps were in good agreement. The positions of 8 genetic markers previously shown on the physical and genetic map were directly confirmed by BAC-endsequence data (Table 2, FIG. 3). The position of 43 from 47 Y-clones (91%) shown on the physical and genetic map, which were later shotgun sequenced, was confirmed by the BAC endsequences and shotgun sequence data. Four clones (Y63, Y180, Y251, and Y253) were located to different positions than previously thought and this was found to be due to book keeping errors or to chimeric inserts. Their present approximate location relative to the oriC is shown in FIG. 3: Y63 at 380 kb, Y63A at 2300 kb, Y180 at 2160 kb, Y251 at 100 kb, and Y253 at 2700 kb. A total of 48 BACs, covering regions of the chromosome, not represented by cosmids were then shotgun sequenced (Cole et al., 1997), and these are squared in FIG. 3. No chimeric BACs were found, which is consistent with the observations of other research groups for other BAC libraries (Cai et al., 1995; Zimmer et al; 1997). The absence of chimeric BACs was of particular importance for the correct assembly of the M. tuberculosis H37Rv sequence. The exact position of the BAC termini sequences on the chromosome will be available via the world wide web http://www.pasteur.fr/MycDB).
    TABLE 2
    Identities of genetic markers previously shown on the integrated
    and genetic map of H37Rv (Phlipp et al., 1996b) wich showed
    perfect sequence homology with BAC ens. sequences.
    BAC GenBank
    end Description of Accession
    Locus sequence genetic marker Organism n°
    apa Rv163SP6 Secreted M. tuberculosis X80268
    alanine-proline-
    rich antigen
    dnaJ, dnaK Rv164T7 DnaJ hsp M. leprae M95576
    fop-A Rv136T7 Fibronectin M. tuberculosis M27616
    binding protein
    pa/A Rv401T7 DNA polymerase M. tuberculosis L11920
    I
    ponA Rv273T7 Penicillin binding M. leprae S82044
    protein
    pstC Rv103T7 Putative M. tuberculosis Z48057
    phosphate
    transport receptor
    recA Rv415SP6 Homologous M. tuberculosis X58485
    recombination
    wag9 Rv35SP6 35-kDa antigen M. tuberculosis M69187
    • Example 5 Repetitive Endsequences
  • Repetitive sequences can seriously confound mapping and sequence assembly. In the case of the BAC endsequences, no particular problems with repetitive sequences were observed. Although nine clones with one end in an IS1081 (Collins et al., 1991) sequence were identified, it was possible to correctly locate their position on the map using the sequence of the second terminus. Moreover, these BACs were used to determine the exact locations of IS1081 sequences on the map. Five copies of this insertion sequence, which harbors a HindIII cleavage site, were mapped on the previous physical and genetic map. In contrast, BAC endsequence data revealed an additional copy of IS1081 on the M. tuberculosis H37Rv chromosome. The additional copy was identified by six clones (Rv27, Rv118, Rv142, Rv160, Rv190, Rv371) which harbored an identical fragment linking Y50 to 1364 (FIG. 3, at ˜1380 kb). This copy of IS1081 was not found by previous hybridization experiments probably because it is located near another copy of IS1081, localized on the same DraI fragment Z7 and AsnI fragment U (FIG. 3, at ˜1140 kb). Furthermore, the position of a copy of IS1081 previously shown in DraI fragment Y1 (FIG. 3, at ˜1840 kb) had to be changed to the region of Y349 (FIG. 3, at ˜3340 kb) according to the endsequences of BAC Rv223. The positions of the four other IS1081 copies were confirmed by the sequence data and therefore remained unchanged. In total 6 copies of IS1081 were identified in the H37Rv genome in agreement with the findings of others (Collins et al., 1991).
  • In addition, a sequence of 1165 bp in length containing a HindIII site was found in two copies in the genome of H37Rv in different regions. The endsequences of BAC clones Rv48 and Rv374, covering cosmid Y164, as well as Rv419 and Rv45, that cover cosmid Y92, had perfect identity with the corresponding parts of this 1165 bp sequence (FIG. 3, at ˜3480 kb and ˜900 kb). Analysis of the sequence did not reveal any homology with insertion sequences or other repetitive elements. However, as each of the two locations showed appropriate BAC coverage, chimerism of the sequenced cosmids Y164 and Y92 can be ruled out as the probable cause.
    • Example 6 Using BAC Clones in Comparative Genomics
  • The minimal overlapping set of BAC clones represents a powerful tool for comparative genomics. For example, with each BAC clone containing on average an insert of 70 kb, it should be possible to cover a 1 Mb section of the chromosome with 15 BAC clones. Restriction digests of overlapping clones can then be blotted to membranes, and probed with radiolabelled total genomic DNA from, for example, M. bovis BCG Pasteur. Restriction fragments that fail to hybridize with the M. bovis BCG Pasteur DNA must be absent from its genome, hence identifying polymorphic regions between M. bovis BCG Pasteur and M. tuberculosis H37Rv. The results of such an analysis with clone Rv58 (FIG. 3, at −1680 kb) are shown here. This clone covers a previously described polymorphic genomic region between M. tuberculosis and M. bovis BCG strains (Philipp et al., 1996a). EcoRI and PvuII digests from clone Rv58, fixed on nitrocellulose membranes, were hybridized with 32P-labelled total genomic DNA from M. tuberculosis H37Rv, M. bovis (ATCC 19120), and M. bovis BCG Pasteur. FIG. 4 presents the results of this analysis, where it is clear that several restriction fragments from clone Rv58 failed to hybridize with genomic DNA from either M. bovis or M. bovis BCG Pasteur. On the basis of the various missing restriction fragments, a restriction map of the polymorphic region was established and compared to the H37Rv sequence data. The localization of the polymorphism could therefore be estimated, and appropriate oligonucleotide primers (Table 1) were selected for the amplification and sequencing of the corresponding region in M. bovis. The alignment of M. bovis and M. tuberculosis H37Rv sequences showed that 12,732 bp were absent from the chromosomal region of the M. bovis type strain and M. bovis BCG Pasteur strain. The G+C content of the polymorphic region is 62.3 mol %, which is the same as the average genome G+C content of the M. tuberculosis genome, hence indicating that this region is not a prophage or other such insertion. Subsequent PCR studies revealed that this segment was also absent from the Danish, Russian, and Glaxo substrains of M. bovis BCG, suggesting that this polymorphism can be used to distinguish M. bovis from M. tuberculosis. Analysis of this sequence showed that 11 putative open reading frames (ORFs) are present in M. tuberculosis, corresponding to ORFs MTCY277.28 to MTCY277.38/accession number Z79701-EMBL Nucleotide Sequence Data Library (FIG. 5). FASTA searches against the protein and nucleic acid databases revealed that the genes of this region may be involved in polysaccharide biosynthesis. Among these putative genes, the highest score was seen with ORF 6 (MTCY277.33), whose putative product shows a 51.9% identity with GDP-D-Mannose dehydratase from Pseudomonas aeruginosa (accession number U18320—EMBL Nucleotide Sequence Data Library) in a 320 amino acid overlap. The novel M. bovis sequence of the polymorphic region was deposited under accession number AJ003103 in the EMBL Nucleotide Sequence Data Library.
  • As it appears from the teachings of the specification, the invention is not limited in scope to one or several of the above detailed embodiments; the present invention also embraces all the alternatives that can be performed by one skilled in the same technical field, without deviating from the subject or from the scope of the instant invention.
    TABLE 3
    End sequences of the polynucleotide inserts cloned in the named recombinant
    BAC vectors contained in the I-1945 M. tuberculosis H37Rv genomic DNA library.
    RvXXXSP6 corresponds to the SP6 endsequence of the clone RvXXX;
    RvXXXT7 corresponds to the T7 endsequence of the clone RvXXX.
    RvXXXIS 1081 corresponds to a region located close to a copy of the IS1081
    repetitive sequence (Insertion element)
    The character << - >> denotes an uncertain base residue.
    Clone Rv101
    :::::::::::::::Rv101SP6.seq::::::::::::::
    AATACTCAAGCTTGCCCAGCCGTCGATGACAAGAAATATGTCCGCAAAAGACTCAGCGGCCGACTTTGCTCGCAGCTGGCGGTACCGCGCCACCGATTCTATGCCG
    TGGTCGCGGAAAAATGCCTCCCGAAATCGCACGGCCGACTCCAGTTCGGCGAGCATCCGCGATGCCAGCTGCGGCTGCGCCCTGCCGGCCACGGCACCCTCATGCG
    GCAGTTCGTCCACCTGGGCCAGCGCCCCGCCGCCGAATTCCAAACAATAGAACTGCACCCGGCCCGCATCGTGGGTAACAGCCAACGGCATGATCAGCGTCGGCAG
    CGCGGTTGACTTGCCCGTTTGCGGTGCACCTACGAACGCGACATTGCCTGCGGCCCCGGACAAGTCGATCGTGCGCGGCACCCGTGACTGCTCTAACGGGCGATTG
    AAATTCCGAT
    :::::::::::::::Rv101T7.seq:::::::::::::::
    CCACCCGTGTAATTTGGGATGGGCAAAAAGGCGAAGCACCGCGTGGCCACGAACGCCGGGAGGGACAATCTCGGGCGGTTAGGGCTTCTCGCGGGAAGGCCCGAAC
    GTACGGCGTTTCAACACCTCGCGTCGCCCTCCGACCGCGAACATTCGGGGATGGCAGCAACCTGCTGGCACCCTGGCCGGGCGATGATCTGCAGCGTCGCCGCGGG
    TAGTCGCCGCCCGGGCGGCTACACTCTGAAACGCGATGACCATCGATGTGTGGATGCAGCATCCCGACGCAACGGTTCCTACACCGCGATATGTTCGGCTCGCTGC
    CCCGGTGGACCGGT
    Clone Rv102
    :::::::::::::::Rv102SP6.seq:::::::::::::::
    AATACTCAAGCTTTCCGCCGATACCCGCCATGTCGCGCACATCCAGGACTTCTGGGGGGATCCGCTGACAGCGGCGGGATCCCAAAGTGCGGATGATCGGGCCGCC
    TACGTCGTGGTGTACCTCGTCGGTAACAACGAAACCGAAGCGTATGACTCGGTCCACGCGGTGCGGCACATGGTGGACACCACACCGCCACCGCACGGGGTGAAGG
    CCTATGTCACCGGTCCGGCAGCACTCAATGCCGACCAGGCCGAGGGCGGAGACAAAAGTATCGCTAAGGTCACCGCGATCACCAACATGGTGATCGCAGCAATGTT
    GCTAGTGATCTATCGCTCCGTAATTACCGCGGTTCT
    :::::::::::::::Rv102T7.seq:::::::::::::::
    GTGCCGTTCCAACCCGAATTGGCTTTCGGCGCCATCGGTGAGGACGGCGTGCGGGTGCTCAACGACGACGTCGTCCGCGGGACACACCTCGATGCTGCCGCCATGG
    ACGCGGTCGAACGCAAGCAGCTGATCGAGCTACAACGCCGCGCGGAACGCTTCCGCCGCGGGCGTGACCGCATCCCGTTGACCGGGCGGATCGCGGTGATCGTGGA
    TGACGGCATCGCCACCGGAGCGACGGCCAAGGCGGCGTGCCAGGTCGCCCGGGCGCACGGTGCGGACAAGGTGGTGCTGGCGGTCCCGATCGGCCCAGACGACATC
    GTGGCGAGATTCGCCGGGTACGCCGATGAGGTGGT
    Clone Rv103
    :::::::::::::::Rv103SP6.seq:::::::::::::::
    AATACTCAAGCTTTCGGCGGAAACGGACACACATTGAATATTGATGACAAAATAAAAATCATTGATGGTTTGAGTCACCAGGCCGATCAAGCCTTCGCCGAGCCAA
    ATTCCAATCAAGAGGCCCAAGCCCGTACCAATCAGCCCGGCAACGAGGGATTCCGTCATTATCAGCCAAAATAACTGCTCTCGGGTTACACCCAAACAGCGCAATA
    TGGCGAAAAACGGTCGCCGTTGCACGACATTAAATGTCACGGTATTGTAGATTAAAAAGATACCCAC
    :::::::::::::::Rv103T7.seq:::::::::::::::
    TGCTCCCGAAACCTGGGGGTGTGCCTGCTCTGTATGCACGGCATACGGACATCCTTCCCCTGAGACCCGCGGTCGAACCAGCCACGTGTCCATCATAGNGGGTCAA
    CCCCGGCCAAGGGCGACGGCACGCCAAGTTCGCCGACCGTTAACCTAGTGCTGTTAGCTTCATTTGCTGCGATCAAAACAGCTGGTCGGCCGTTAGGAACTGAATT
    GAAACTCAACCGATTTGGTGCCGCCGTAGGTGTCCTGGCTGCGGGTGCGCTGGTGTTGTCGGCGTGTGGTAACGACGACAATGTGACCGGGGGAGGTGCAACCACT
    GGCCAGGCGTCGGCAAAGGTCGATTGCGGGGGGAAGAAGACACTCAAAGCCAGTGGGT
    Clone Rv104
    :::::::::::::::Rv104SP6.seq:::::::::::::::
    ATACTCAAGCTTTGCCGACGAGCGGGCGATGTTGATGACGGGAAACCCCAGCGCACAACCGACGATTTTGGCGTAGCCGGCGGACGTCTGCTCGATTCCGATCACG
    TCGGCGCTCGCATCGAGCATGGCGCCGGCGACGGCTAGCAGCGATCCGCCGTCGTCGAGAAGCACGACACGAGCCGTACGCCCGGCCGTAAGCCGCGCCCAGGATT
    CGGCGAAAAACCGTTCTACGTGGCGGGTGTACTGGGTGTCGAATGATTCGTGGGGTGCGTAGGCGTCGCTGCAATCGTCGACATAGATGCCGTCGGGCCGCATCGC
    GTCGACAACTCCGGGTGAGTGCAATAGCACTTGCCGATCACCGCGACGTTGCGCGGATGAGGCCGAACCCGAATA
    :::::::::::::::Rv104T7.seq:::::::::::::::
    TCCTATGTCCCTGCCGAGCANGTGATCGAACGCGGTGACAGATTTGTCTATCCTGGACCTGACGGTGAGGTCGAAGTTTTCCAGGAATTCGGCAAAATCGGTAAGA
    GCCTGAAGAATTCGGTATCGCCGGACGAAATCTGCGACGCATACGGGGGCATATACGCTTCGGGTTTACGAGATGTCGATGGGGCCGCTGGAGGCTTCACGTCCAT
    GGGCCACAAAGGATGTTGTCGGCGCGTACCGTTTTCTGCAGCGGGTGTGGCGCTTGGTCG
    Clone Rv105
    :::::::::::::::Rv105SP6.seq:::::::::::::::
    ATACTCAAGCTTGATTCCGCCGAAACCGACCGTGAGCACCCCGCCAGCCACCACGCTCGGGTCGGGCGCCGGGCCCGGGCCGCCAGGCTGCTCCGCTCGGTGATGG
    CACGCCACCGCGACACCACCCGGCTGCGCTACGTCTAACCATTCCAGGCGGAGCTACATCAGCTCGGCCGCCCAGTGTTCGGGGCCTCTTTCCAGGTCGAAGTCTA
    TACCGATATGCGCATCCGCAGCCGCCACCCTGGAGAACAGAACGATGCCCTACTAATGCTTGTCTGGCGGGGCC
    :::::::::::::::Rv105T7.seq:::::::::::::::
    GGTACGCTTCGGTCGCAGTCTGCGAGTGATGCATGACGACCGGGACCTCGTCGGCATCTTCCATAGCCCGCCACACCTTCAGTTGCTCACCGGAATCCAACCGGTA
    GAAGGTCGGCGAGCGCTCGGCATTGGTCATCGGGATATGCCGCTCGGGACGGTCAGAGCCCTCGGGTCCGGCCAGCACTCCGCAGGCTTCGTCGGGGTGGTCGCGA
    CGCGCATGGGCCACCATCGCATTCACCAGGTCTGCGCGAATCACCAGCACGTAGACGGTTCCTTTCCTAAGCAACACCGAAGTTTCAGGACCCGAATGCTCCGGGA
    AACATGTCACGGTAGGTCGGTATTCCGGCTACCGGCTGA
    Clone Rv106
    :::::::::::::::Rv106SP6.seq:::::::::::::::
    GGCGTCAACGGTGTCGGAACCCGCGTCAAGCAATTGGTAGGCCTGCAGTCTGTGAATCAGGCCGACGCTGTGGCCGCCGCGGC
    :::::::::::::::Rv106T7.seq:::::::::::::::
    GGCTNGCGTACCCGGTACCGGCCGCGGGCCTACCACGTGCCGGAACTGGAAGCGCAGTAAGCCCTCAACGCGCCACCGCTTTGGCCCGGGGGCCCGGCGTAGGCGC
    ATCGGCGGTGGCCGTGGGGCGGCGCACTGCGACCTCACCAGCGGCTTTCGAGCTTTGTTCGATCAACCGGCCAGCATGGTCGANGATGCATTCGAGACCATATTCG
    AAATTGGTTTCATCGGGGGCCCCGATCCGATGCCCCCTCCCAGTTGCGTGAGCAANCAGCGGAGTCNTCGCGGGATCGATGGCCACGGGGTGTTCAATGGCGGATG
    GTCCGCTGCCCGCCGACTGGCTCTTGCGGGAGAACCGATCTAGCACCACCGATCCGCGCACGTNG
    Clone Rv107
    :::::::::::::::Rv107T7D4.seq:::::::::::::::
    CGTAATNTCGCGCACANCCANGACTTCTGGGGGGATCNGCTGACAGTGGTNGGATCCCAAATTGCGGATGATCGGGCCGCCNACGTCGTTGTGTACCTCNTCNGTC
    ACAACNAANCCGAANCGTATGACTCGGTCCACGCGGTGCGGCACATGGTGGACACCACACCGCCACCGCNCGGGGTGAAGGCCTATGTCACCGGTCCGGCAACACT
    CAATGCCGACCAGGCCGANGCCGGACACNANAGTATCNCTAACGTCACCGCGATCACGAGCATGGTGATCGNNCAATGTTNCTANTGATCTATCGCTCCGTAATTA
    CCGCGGTTCTCGTCTTGATCATGGTCGCANCGAACTCCGGCGCAATCCGCGGATTCATCGNCTTGCTCGCCGATCACATATTTTCAGCCTTTCACATTGCAACNAA
    CCTGCTCGTCTCATGGNGATGCGGCGACACGGACTACCGATATCATGCTCGCCGTTACACAATCNCGCCACGCCGCGAAGACNGGAAACGCTTCTACACAATNTTC
    NCGGGACGCCACTNAACTTGGTTCNGGTTTGACATTGCCGCGCATGTNTGCCCAGCTTTGCCGGCTCCCCTTA
    Clone Rv108
    :::::::::::::::Rv108T7D4.seq:::::::::::::::
    TGAATTTCCCGATCCCACAATCTCGGTTCAGATACAGGTCGCCATACCCCTTACTTCGGCAACGCTGGGCGGATTGGGGCTGCNGCTGCAGCANACCATCGACGCC
    ATCGAATTGCCGGCAATCTCGTTCAGCCAATCCATACCCATCGACATTCCGCCGATCGACATCCCGGCCTTCNCCCTTTAACGG
    Clone Rv109
    :::::::::::::::Rv109SP6.seq:::::::::::::::
    AACAGCTATGACCATGNTTACGCCAAGCTATTTAGGTAACACTATANAATACTCAAGCTTTTACGGTGATCGCGCATCACCTGGTTCATGAACTGGAAGCAGCGCA
    NCGCTTCCTTTTCGGCCGCAACATGAGCCAGCCTCTCGTCCGCGGTCNGGTGCAGGTGCTCGGGCAGCTCGGCCGCGACAGCCGCCTGACCCTGAAACCAGCTTCC
    ATATCCCGCGACNAACNACNCCAGTCCGCTACGTAACCCCTCCGCGACTGTCCATGGACAACAGCGCGTTCTCCACCGACCGGGCCCGGGTGTGGGGTGTTTGGGC
    GACCGGCAGCCAGGTGGTCCACACTGCCGACGGGCGCCGCGAGCCGTTCACCGACCAAGCCGCCGAACAAGTCCGCCCGATCGCATACTCCAACCGGTTGCGGTAC
    TGCAGGTCAGCTGGCGTACCTCCTCNTCNCGCTCGGCGAAGTCTTGCTCCANCACGTCGCAGAACGGCAAGGAACACGTTCA
    :::::::::::::::Rv109T7seq:::::::::::::::
    GACCGNNCCATGTTTCCACAATGTGGTGCCAGTNCGGNGGCTACGTGCCATCNANACACTGGCGCAGGCTATCGCACCCGTTATCNGCTACGAACAAATCNCGGTA
    TGCGTTCTTTANCATGAGTCGGCGACCGNCGATCATGGTCGACACCCACGACNGAAATACGCAGATCGCCNTCNAGCNTGTGTGCCGCGGATTATCANGACTGACC
    TCCTGGCTGACCGGNNTGTNTGGTCGCGATGCCTGGCGCCCGGCCGGCGTGNTCGTGGTCGGCTCGGATAGCGAAGTCAGCTAATTCTCGTGGCAGCTCGAAAGGG
    TCCTGCCGGTGCCGGTCTTTGCGCAAACCATGCNCATGTTACGGTCCCTCGGGTGCGGCCTGGCGGCGGC
    Clone Rv10
    :::::::::::::::Rv10SP602.seq:::::::::::::::
    GGGATGGGCGGGCCCGCTAAACTCTTCGTGTTCCACTAACTCCGGGAGGGNCAATCTCGGGCCGTTATGGCTCACGTCGCGTCGCCCTCCGACCGCGAACATTCGG
    AGTTGGCAGCAACCTGGTAGCACCCTGGCCGG
    :::::::::::::::Rv107TD4.seq:::::::::::::::
    NCCGTCGTTGACAAGTAAATATGTCCGCAAAAGTCTCAGCGGCCGACTTTGCTCGCAGGTGGCGGTACCGCGCCACCGAGTCGATGCCGTGGTCGCGGAAGAATGC
    CTCCCGAAATCGCACGGCCTTCCCNNTTTAAACGGA
    Clone Rv110
    :::::::::::::::Rv110SP6.seq:::::::::::::::
    TTTAGGTGACACTATAGAATACTCAAGCTTTTGGTCTAGCCGGCCGAGCACGATACGGGTGTCATTGGCCACCGGCGGCGGCTGTCCGGGAAATGGCGGGTCCCCG
    GTGGTTTTGCTGATGAGTGCTGAACCGTANTCGAAGTGGGCGGCGTCAGACTCCACCCANCCAGCAGGCAGCGCGAAGCTGAATCCTCCAACCGGGTTGTCNATCC
    GGACAAGTTGGGGTGCGTTTGGGGCAATGACAGGTGGCNGCGGTGCGTTCGGGTCCGCCGGCCGGAAGTGCTGCGTTGGATCNCCCGCTGGGCATTGGGCNTTTTT
    GCGGCGGCCGGTGGTNGGGGGGCAACAGGTNTCCCNGTGCGGGTGGCGCTCAACGGTCNACGGCGCAAGCCGCCGTTGTTGGTACCNGGGGCGCTGGCTCCGGATC
    GCGTTGGCGGTCNCCGG
    :::::::::::::::Rv110T7.seq:::::::::::::::
    CTACACCATCGAATACGACGGCGTCGCCNACTTTCCGCGGTACCCGCTCAACTTTGTGTCGACCCTCAACGCCATTGCCGGCACCTACTACGTGCACTCCAACTAC
    TTCATCCTGACGCCGGAACAAATTGACGCAGCGGTTCCGCTGACCAATACGGTCGGTCCCACGATGACCCAGTACTACATCATTCGCACGGANAACCTGCCGCTGC
    TAGAGCCACTGCGATCGGTGCCGATCGTGGGGAACCCACTGGCGAACCTGGTTCAACCAAACTTGAANGTGATTGTTAACCTGGGCTACNGCGACCCGGCCTATGG
    TTATTCNACCTCNCCGCCCAATGTTGCGACTCCGTTCGGGTTGTTCCCANAAGTCNNCCCGGTCGTCATCGCCGAANCTCTCNTCCCGGGACCCACAGGGAATCNG
    CNATTTCNCCTACAAATCANCCACCTCCA
    Clone Rv111
    :::::::::::::::Rv111T7.seq:::::::::::::::
    GCATGATCGGCCACCTTTCGGGCCGCCCGGCATACGGCGGCGTACCGATCTCCGCGTCATACACCCGCGGGTAATCGCCGACGGTGCCGGTTCGCGAGCCGAAGGT
    GACGACTCTGATTGAATCGAGTTCCAGGTCCAGCGGGTGGCGCACCAACGGCGCGAGCTCAACGACGTCAATCNCGTTGTCGCTTTCTACGGTCACCGACCCTGGT
    GACCGTATGGCNCCCG
    Clone Rv112
    :::::::::::::::Rv112SP6.seq:::::::::::::::
    GACACTATAGAATACTCAAGCTTGCCAACCGCCAGCCTGCATCCGGCGGCGANCACTGCTCCGCCGACCNGTACGAACCAACCTGCGGTGCCCAGGCCATTGACGA
    TGTGCTGGTCGGCGCCCGCGAGTCCGCGCACCATCAACGCCGCGGGCACCACCANGGCGGCCCCACCCTGCACGGCGACGATCATTCCGGCGCCGCTCACGGCGGG
    CGGGGCTCGAACANGCACAGCATCAACGTNGTCACCCGGCCGTGACCGGCCCGCATCGTCACACCACCCAAGCCCATTGCCGTCCTCCTCAACNGGGCGACCCGGC
    CCGCATCGTCACACGGNCTAAGGCCATTGCCGTCCTCCT
    :::::::::::::::Rv112T7.seq:::::::::::::::
    TCGGCGCCATCGGCACCTTCGAGGACCTGTATTTCGACGCCGTGGCCNACCTGAGGTTGGCGGTGGACNAAGTGTGCACCCGGTTGATTGGCTCGGCCTTGCCGGA
    TGCCACCCNGCGCCTGGTGGTCGATCCGCNAANAGACAANTTGTGGTGGANGCTTCTGCTGCCTGCGACACCCACNACGTGGTGGCACCGGGCAGCTTTAGCTGGC
    ATGTCCTGACCGCGCTGGCCGACNACTCCAGACNTTCCACNAANGGTCGCCNNCCCAATGTNCCGNANTGTCTCCGGNTCCCTTTACCNCCCAATGGGCNGNTTCC
    ACNGGTTACGGGCCCCNTNCCGGCGGGTCTNCCTCCCAANCTACCAAATACGCCCGACNTTCCGGA
    Clone Rv113
    :::::::::::::::Rv113SP6.seq:::::::::::::::
    ATACTCAAGCTTTTATGGTGATCGCGCATCACCTGGTTCATGAACTGGAAGCAGCGCAGCGCTTCCTTTTCGGCCGCAACATGAGCCAGCCTCTCGTCGGCGGTCG
    GGTGCAGGTGCTCGGGCAGCTCGGCCGCGAACAGCCCGGCTTGAACCCTGAAAACCNGCTTTCCATATCCCGCGACGAAAGAACGCCAGTTCCGCTACTTAAGCCC
    TCCGCGAACCGTCCATGGACAACAGCGCGTTCTCCACCAACCGGGCCCGGGTGT
    :::::::::::::::Rv113T7.seq:::::::::::::::
    TCGGCTCAGGCCGCGCTGCTGGTAGAGTCGCTGACCGGTGCAGGTTTCGACAATGTGGTGCCGGTTCGGCGGCTACGTGCCATCGAGACACTGGCGCAGGCTATCG
    CACCCGTTATCGGCTACGAAGCAAATCGCGGTATGCGTTCTTGAGCATGAGTCGGCGACCGTCGTCATGGTCGACACCCACGACGGAAAGACGCAGATCGCCGTCA
    AGCATGTGTGCCGCGGATTATCAGGACTGACCTCCTGGCTGACCGGCATGTTTGGTCGCGATGCCTGGCGCCCGGCCGGCGTGGTCGTGGTCGGCTCGGATAGCGA
    GGTCAGCGAATTCTCGTGGCAGCTCGAAAGGGTCCTGCCGGTGCCGGTCTTTGCGCAAACAATAGCGCAGGTTACGGTCGCGCGGGGTGCGGCCTGGCGGCGGCC
    Clone Rv114
    :::::::::::::::Rv114SP6.seq:::::::::::::::
    CAAGCTATTTAGGTGACACTATAGAATACTCAAGCTTCGCGTCTACGCCGGCCCGGAGCATCCGCACAGCGCTCAGCAGCCGGTTCCGTACGANCTCAAGCAGGTG
    GCGCAATGACCGAAACCACCCCAGCCCCGCAAACCCCGGCGGCCCCGGCCGGGCCCGCACAATCGTTCGTGTTGGAGCGGCCCATCCANACCGTTGGGCGCCGTAA
    GGANGCCGTGGTACGAATGCGGCTGGTGCCCGGCACCGGCAAGTTCGACCTCAACGGCCGCAGCTTGGANGACTACTTCCCAAACAAGGTGCACCAGCAGTTGATC
    AAGGCACCCCTGGTCACCGTGGATCGGGTGGAAAGTTTCGACATCTTTGCCCACCTGGGCGGCGGCGGCCGTCCGGTCAGGCCGGGCCTGCCCTGGGTATCGCCCG
    GGCATTGATTCTGGTATCCCCNGAAGAACCG
    :::::::::::::::Rv114T7.seq:::::::::::::::
    CGGTTGGCCACCGCTTCTGCGGTGCCGCCGCCGTCGACAATGACCGTGTCGTCCTTGCTGACCACCACGCGTCGGGCCGAGCCCAGCACCTCCAAGCCCACCTCGC
    GCAGCACCATGCCGGCGTCGGGGTTGACCACCTGGCCACCCGTCACCACCGCCAGGTCCTCAAGGAAACGCCTTACGGCGGTCACCGAMGTACGGCCCCTTGACCG
    CGACCGCTTTCAACGTCTTGCGAATCGCGTTGACGACCAGCGTCGCCAACGCTTCGCCCTCCACGTCTTCAGCCACGATCAGTAGTGGCTTACCCGTTCCTGCAAC
    CTTTTCCAGCAATGGCAACAGATCGGGAAGCGANCTGATCTTGTCTTGGTGCN
    Clone Rv115
    :::::::::::::::Rv115SP6.seq:::::::::::::::
    CCAAGCTATTTAGGTGACACTATAGAATACTCAAGCTTTTGGCTGGGTCGCCTTCGAATTCNGCGTGCACCGCTATGGGTTGCANCAGCGGCTGGCGCCGCACACC
    CCACTGGCCCGGGTGTTTTCGCCCCGAACCCGGATCATGGTGAGCGAAAAGGANATTCNCCTGTTCGATGCTGGATTCGCCACGCCAAGGCATCTANACGATTACT
    CTCCNCGGGGTGGGAAAAGTGCCCAATCCCCCTCCCTCCAACTTTCCNAACAATCATTCCGGTTCCNCCNTCCGGTTGGNGGTAACCNNCCAATAAAACCCCTGCC
    CG
    :::::::::::::::Rv115T7.seq:::::::::::::::
    GCCCGCNCATGGCCAATCCCCGAAGACATCATTGGCCAGTGGCCGGGCGCTAACAGGTTCCAGCCCCCCACCANTGCCGCTCGAACATGCGGTGCAACCCATTCGC
    AGGCCGGCAGGGAAAGCACCGCGGAAGCCGCAAAGGGCTGCAGTTCCGCGCCCAATAATGTCGTCCGCAACCAGATGCGCTCNAAAACCNCNCCGGCAGTCAGCGC
    ACCCGACGCGANGTCGAAAGACGTCNTCAGCGCGCCCACATGGGGTGCCAATCGGCACGGCAGGTATGCCGCGCGCAACCCGAGCGCGTGGTGCATGCCCACGGTC
    CGCANGANGCGCANCACCCGCCAATGCCGAANCCCACGAAACATCGGGCGCATCCACCTTCAACC
    Clone Rv116
    :::::::::::::::Rv116SP6.seq:::::::::::::::
    ATACTCAAGCTTGCCCAGCCGTCGATGACAAGAAATATGTCCGCAAAAGACTCAGCGGCCGACTTTGCTCGCAGCTGGCGGTACCGCGCCACCGAGTCGATGCCGT
    GGTCGCGGAAGAATGCCTCCCGAATTCGCACGGCCAATTCCATTCCGGGAAGCATCCGCAATGCCAGCTGCGGTTGCCCCCTGCCGGCCACGGCACCCACTTGCGG
    CATTGCGTCCACCTGGGCCAGCGCCCCGCCGCCAAATTCCAAACAATAAAAATTGCACCCGGC
    :::::::::::::::Rv116T7.seq:::::::::::::::
    CCACCCGTGTATTTTGGGATGGGCAAAAAGGCGAAGCACCGCGTGGCCACGAACGCCGGGAGGGACAATCTCGGGCGGCTAGGGCTTCTCGCGGGAAGGCCCGAAC
    GTACGGCGTTTCAACACGTCGCGTCGCCCTCCGACCGCGAACATTCGGGGATGGCAGCAACCTGGTAGCACCCTGGCCGGGCGATGATCTGCAGCGTCGCCGCGGG
    TAGTCGCCGCCCGGGCGGCTACAGTCTGAAACGCGATGACCATCGATGTGTGGATGCAGCATCCGACGCAACGGTTCCTACACGGCGATATGTTCGCCTCCCTGCC
    CCGT
    Clone Rv117
    :::::::::::::::Rv117SP602.seq:::::::::::::::
    CTGCCCATGTTTGGGGACGCCCGACCAGCCGATGCTGGAGGCCTACACGGCCCTTGGTGCGCTGGCCACGGCGACCGAGCGGCTGCAACTGGGCGCGTTGGTGACC
    GGCAATACCTACCGCAGCCNGACCCCTNTCNCAANAGGATNTTGTTCGCCGGACCCCNCTC
    :::::::::::::::Rv117T7D4.seq:::::::::::::::
    CCGACTTTCCGCGGTACCCGCTCAACTTTGTGTCGACCCTCAACGCCATTGCCGGCACCTACTACGTGCACTCCAACTACTTCATCCTGACGCCGGAACAAATTGA
    CGCAGCGGTTCCGCTGACCAATACGGTCGGTCCCACGATGACCCAGTACTACATCATTCGCACGGAGAACCTGCCGCTGCTAGAGCCACTGCGATCGGTGCCGATC
    GTGGGGAACCCACTGGCGAACCTGGTTCAACCAAACTTGAAGGTGATTGTTAACCTGGGCTACGCGACCGCCTTT
    Clone Rv118
    :::::::::::::::Rv118SP6.seq:::::::::::::::
    ATACTCAAGCTTTGTCACACCAAGTGTTTCGACCAGGCGCTCCATCCGGCGAGTGGATACTCCCAGCAGGTAGCAGGTCGCCACCACGCTGGTCAGTGGGCGTTCA
    GCTCGCTTGCGGCGCTGCAGCAGCCATTCGGGGAAATACCTGCCCTGGCGCAGCTGGGGGATCCCAACTTCAATGGTTGCGGCACGGGTGTCAAATTCACGGTGGC
    GGTAGCCGTTGCCCTAATTGGACCGCTCATCGCTGCTTTCGCGGTACCCCGCCCCGCACAGGGCTTCGGCTTCAGCCCCCATCAGGGCGGCAATAAACTTCAAGAG
    CACC
    :::::::::::::::Rv118T7.seq:::::::::::::::
    GAGGCAGCTTCGCCGGCAATTCTACTAGCGAGAAGTCTGGCCCGATACGGATCTGACCGAAGTCGCTGCGGTGCAGCCCACCCTCATTGGCGATGGCGCCGACGAT
    GGCGCCTGGACCGATCTTGTGCCGCTTGCCGACGGCGACGCGGTAGGTGGTCAAGTCCGGTCTACGCTTGGGCCTTTGCGGACGGTCCCGACGCTGGTCGCGGTTG
    CGCCGCGAAAGCGGCGGGTCGGGTGCCATCAGGAATGCCTCACCGCCGCGGCACTGCACGGCCAGTGCCGCGGCGATGTCAGCCATCGGGACATCATGCTCGGGTT
    CATACTCCTCGACCAGTCGGCGGAACAGCTCGATTCCCGGACCGCCCAGCGCATTGGTGATGGAATCGGCGAACTTGGCCACCCGCTGGGTGTTGACATCCTCGAC
    GGTGGGCAATTGCGCCTCGGTAAGCTTTGCCGCGTAGCCTTTTCATC
    Clone Rv119
    :::::::::::::::Rv119SP6.seq:::::::::::::::
    ATACTCAAGCTTCACTGACAAGGGACGAATTCGTCGGCCGCCTGTTCGACTGGGTGGTGGCCGAGCTGGTCGCCACCACTCAGGCCGCGGTCACGGCGGTACCGGC
    GCGGGAGCAAACTCGCGCGGGCATGGCCAACTTCTTGCGGACCATCACCGCAGACGCCCGCTTCGGACCCCTGCTGTCCACCACACAGTTGGCCAACGCATTAATC
    ACCCGCAAGCTTGCGGAATCCACCGCCCTGTTCGC
    :::::::::::::::Rv119T7.seq:::::::::::::::
    TCCATCACCCGATGTGGCNGGAGCACTGCCATGTCGATCTCAACTACCACCTCCGGCCGTGGCGGTTGCGCGCCCCGGGGGGTCCGCGCGAACTCGACGAGGCGGT
    CGGAGAAATCGCCANCACCCCGCTGAACCGCGACCACCCGCTGTGGGAGATGTACTTCGTTGAGGGGCTTGCCAACCACCGGATGCGGTGGTTGCCAAAAATTCAC
    CATGCGTTGGCTGACGGTGTTGCCTCGGCAAACATGATGGCACGGGGGATGGATCTGCCGCCGGGACCGGAGGTCGGCCGCTATGTGCCTGACCCCGCTCCTACCA
    AGCGGCA
    Clone Rv11
    :::::::::::::::Rv11SP6.seq:::::::::::::::
    AGCTTTGCAGTTGCTGAGTAATGTCGGCCAACGTCACCACAACCGCGATGAATTCAATCATGCCGCCCAGGGCGGCCAACCCAATGGTGGCCGCGAGCGGCAGCTC
    GATCGCAGCGCGGAGGTTGCCGGCCGCCAGTTGATTCACGAACAGGGTGAGGTCATAGGCGGGCAGGATAGTGACGAAGGCAAGACCTCCATCTGCCGTCGGAAGA
    AGTATCGAG
    :::::::::::::::Rv11T7.seq:::::::::::::::
    AGCTTCAGAACAGGCCTGTTGTGGGCGCACCCGGCTCGCCGAGTTCTGCACGCACCGCCTCAAGTGCGGCCCGCACCGCCGGCATCTCCCGGTCAGGCAGGGCCGC
    GGCCCGCGCCGCAGCGACGGCGTGTTCGCGCAGTTCGCCGTCAATGATGCTGACCTGATCGGCCACCCGGGCGTTCTCGGCGTCGTCGCGTTCACTAATCGCGGTG
    CTCAGCAGCGTCTCGACAGCCACCACCCGAGTGGCGACCAGCTGCTCCACCACGGACCGCAGCGATGCCCGTC
    Clone Rv120
    :::::::::::::::Rv120SP6.seq:::::::::::::::
    ATACTCAAGCTTCAGTTCCTCCACGACGCGTTCCCAAATGAATTTCCCGATCCCACAATCTCGGTTCAGATACAGGTCGCCATACCCCTTACTTCGGCAACGCTGG
    GCGGATTGGCCCTGCCGCTGCACCAAACCATCAACGCCTTCAAATTGCCGGCAATCTCGTTCAGCCAATCCAT
    :::::::::::::::Rv120T7.seq:::::::::::::::
    GCTCTACGCCGCCTACGGGTCGAACATGCATCCCGAGCAGATGCTCGAGCGCGCACCCCACTCGCCGATGGCCGGAACCGGCTGGTTACCCGGGTGGCGGCTGACG
    TTCGGCGGCGAGGACATCNGCTGGGAAGGGGCGCTTGCCACCGTCGTCNAAGACCCAAATTCGAAGGTGTTCGTCGTGCTCTACGACATGACCCCGGCGGACGAGA
    AGAACCTTGACCGGTGGGAAGGCTCCGAGTTCGGTATCCACCAGAAGATCCGATGCCGCGTGGAGCGCATTTCCTCGGACACCACAACGGGATCCCGTCCTCG
    Clone Rv121
    :::::::::::::::Rv121SP6.seq:::::::::::::::
    ATACTCAAGCTTGCCAAAGAGACCTCGTCCACCAAGCAGGACGCGACCGTCGAGGTGGCGATCCGGCTTGGCGTCGACCCGCGTAAGGCAAACCAGATGGTTCGCG
    GCACGGTCAACCTGCCCACACCGGCACTGGTTAAGAACTGCCCGCGTCGCGGTTTTCGCGGTTGGTGAAAAGGCCAATGCCTGCGTTTGCCGTGGGGGCGGATGTT
    GTCGGGAGTGACAATCTGATCAAAAGGATTCAGGGCGGTTGGCTGGAATTCAATGCCGCAATCGCGACACCGG
    :::::::::::::::Rv121T7.seq:::::::::::::::
    CCACGGCGTGGATCAAGGTACCGGCCGGGATGTTGCGCAATGGCAGGTTGTTGCCCGGCTTGATGTCGGCGTTAGCGCCGGATTCCACCACATCCCCTTGCGAAAG
    TCCGTTGGGTGCAATGATGTAGCGCTTCTCCCCATCGAGATAGTGGAGCAACGCAATCCGTGCGGTACGGTTCGGGTCGTACTCGATGTGCGCGACCTTGGCGTTG
    ACACCATCTTTGTCATTGCGGCGAAAGTCGATCATCCGGTAAGCGCGCTTATGACCGCCGCCTTTGTGCCGGGTGGTAATCCGGCCATGCGCGTTGCGTCCACCGC
    GACGTGCAGCGGGCGCACCAGCGACTTCTCCGGGGTTGACCGGGTNATCTC
    Clone Rv122
    :::::::::::::::Rv122SP6D2.seq:::::::::::::::
    GCAGCATGACGGCGGTAGCGAACACCGCCGGATGCAGCGCAAGTAGCGTCGATGTGCTCACGGAATCGCCCCGGCACCGCGATCTCGANGATCACCAGTGCCACCC
    CCTGCAGCGCNACACCGACGATTCCGTACACCGCCACGCCGATCAGGCCCTGGGCCATCTGATTGGAGCTGGCGTANATGGCGGCGATGGTGACGATGGCCAGCGC
    CACATACATTGTGGCGGCCAGAACCACGGCGTTGGGGCGGCGGTCGATGAACACTAGGCGACGCAGATCGCCCGGGGTCAACAGGTTGACCATCAGAAAGCCTGCG
    ACTAGCACGGCGGCGCCACTAGGAAGTACAAGAANGTGGCCACCACCCCATGCAGGATCGGGGTAAGGCTGATGGTCCCGAAATCGACTCCGGCCTAATACATGAC
    TCTCTCCTTTGCGTCATCGCCTTACTTGTGCGCGGAA
    Clone Rv
    :::::::::::::::Rv123SP6D2.seq:::::::::::::::
    GGGACACACCTCGATGCTGCCGCNATGGACGCGGTCGAACGCAAGCAGCTGATCGAGCTACAACGCCGCGCGGAACGCTTCCGCCGCGGGCGTGACGCATCCCGTT
    GACCGGCCGGANCNCTCTCTA
    :::::::::::::::Rv123T7D4.seq:::::::::::::::
    TGGGCGCCTCTTTCGGCCTTCCCNNTTTAAACGNAGCANGACATTCTGGGTATCGAGTTGTACTGGATGGTGTTGGCGATGTCGGTGATCCTGCTCCTGGCGGTGG
    GATCCGACTACAATCTGCTGCTGATTTCCCGGTTGAAAGAGGAAATTGGGGCCGGATTGAACACCGGAATTATCCGTGCCATGGCTGGTACCGGGGGAGTGGTGAC
    GGCTGCCGGCATGGTGTTCGCCGTTACCATGTCGTTGTTTGTGTTCAGCGATTTGCGAATTATTGGTCAGATCGGTACCACCATCGCCTTCCC
    Clone Rv124
    :::::::::::::::Rv124SP6D2.seq:::::::::::::::
    CCGATCGGCGCCGCANCTGGTTGGTGTTNCGGATGAATCCGCAGCGAAAATGTAGCTGCGGTGGCGTGTCGTGACTCGTNGGCGTCGACGCTCGTGGCAGCCACCG
    ANCGGTTGGTCCAGGATCTGGATGGGCAAAGTTGTGCGGCCCGGCCGGTGACGGCCGATGAGCTGACCGAGGTCGACAGCGCCGTGTTGGCTGACTTGGAACCGAC
    ATGGAGTCGCCCCGGTTGGCGTCACCTCAAGCATTTCAATGGTTATGCGACCAGTTTTTGGGTTACGCCGTCAGACATCACGTCGGAGACTTGGATGAGCTGTGTC
    TGCCAGATAGCCCCGAATCGGGACGACCGTGGTCACGGTGCGTCTGACCACTCGGGTCGGGTCGCCCGCGCTATCGGCATGGGTGCGTNATCACAGCGACACGCGC
    CTGCCCAAGGANGTNCGGNCGGACC
    :::::::::::::::Rv124T7D4.seq:::::::::::::::
    CGGGTTGCGGATCCACGCGTGCGGGTTGTCAGCAGCTACGGCACTGAACCGCGGCCACAGCTCGCCGATCCGCTTTCGGTGGTTCTCGATCGACTCGCCGTAGGCG
    ATGCGCAGCGCCTGCTCGAATATCGGGTACACGTAGGCCGGCCTTCCCNCTTTA
    Clone Rv126
    :::::::::::::::Rv126SP6.seq:::::::::::::::
    CTTGATTTTGATCATCATGACGATCATCACCCTAATTTTGCTACCCGCACTGGTTATCGTGGGTACCGTCGTGCTTTCCATGGGCGCCTCTTTCGGGCTTTCGGTA
    TTGGTCTGGCAGGACATTCTGGGTATCGATTTGTACTGGATGGTGTTGGCGATGTCGGTGATCCTGCTCCTGGCGGTGGGATCCGACTACAATCTGCTGCTGATTT
    CCCGGTTGAAAAAGGAAATTGGGGCCGGATTGAACACCGGAATTATCCGTGCCATGGCTGGTACCGGGGGAGTGGTGACGGCTGCCGGCATGGTGT
    :::::::::::::::Rv126T7.seq:::::::::::::::
    GGGGATCCCTAGATCGACCTGCAGGCATGCAAGCTTGGCGTGTCGTTCCAACCCGAATTGGCTTTCGGCGCCATCGGTGAGGCGGGACACACCTCGATGCTGCCGC
    CATGGACGCGGTCGAACGCAAGCAGCTGATCGAGCTACAACGCCGCGCGGAACGCTTCCGCCGCGGGCGTGACCGCATCCCGTTGACCGGGCGGATCGCGGTGATC
    GTCGATGACGGCATCGCCACCGGAGCNACTGTCAAGGCGGCGTGCCAGGTCGCCCGGGCGCACGGTGCGGACAAGGTGGTGCTGGCGGTCCCGATCGGCCCAGACG
    ACATCGTGGCGAGATTCGNCGGGTACGCCGATGAGGTGGTGTGTTTGGCGACGCCGGCGTNGTTCTTCGCCGNCGGGCANGGTTACCGCAACTTCACCCAGACCTC
    CGACGACGAGGTGGTGGCGTCTCCTGGATCGTGCTC
    Clone Rv127
    :::::::::::::::Rv127SP6.seq:::::::::::::::
    AAGGCTGCAGGTCGAAGCGGNTGGTTACGACTCCCTGTGTGTGATGGACCAGTTCTACTATCTGCGTCTACACGGCCCTTGGTGCGCTGGCCACGGCGACCGAGCG
    GCTGCAACTGGGCGCGTTGGTGACCGGCAATACCTACCGCAGCCCCGACCCTGCTGGCAAAGATNATCACCACGCTCGACGTGGTTAGCGCCGGTCGAGCGATCCT
    CGGCATTGGAGCCGGCGGGTTTGAACTGGAACACCGCCAGCTCGGCTTCGAGTCCGGCACTTCCAGTGACCGGTTCAACCGGCTCGA
    :::::::::::::::Rv127T7.seq:::::::::::::::
    CTTTCCGCGGTACCCGCTCAACTTTGTGTCGACCCTCAACGCCATTGCCGGCACCTACTACGTGCACTCCAACTACTTCATCCTGACGCCGGAACAAATTGACGCN
    GCGGTTCCGCTGACCAATACGGTCGGTCCCACGATGACCCAGTACTACATCATTCGCACGGAGAACCTGCCGCTGCTACAGCCACTGCGATCGGTGCCGATCGTGG
    GGAACCCACTGGCGAACCTGGTTCAACCAAACTTGAAGGTGATTGTTAACCTGGGCTACGGCGACCCGGCCTATGGTTATTCGACCTCGCCGNCCAATGTTGCGAC
    TCCGTTCGGGTTGTTCCAGANGTCAGCCCGGTCGTCATCGCCGACGCTCTCGTCN
    Clone Rv128
    :::::::::::::::Rv128SP6.seq:::::::::::::::
    CGGTCATAGCCCTCGGGTCCGGCCAGCACTCCGCAGGCTTCGTCGGGGTGGTCGCGACGCGCATGGGCCACCATCGCATTCACCAGGTCTGCGCGAATCACCAGCA
    CGTAGACGGTTCCTTTCCTAAGCAACACCGAAGTTTCACGACCCGAATGCTCCGGGAAACATGTCACGGTAGGTCGGTATTCCGGCTACCGGCTGAGCATTGAGCA
    CGCCGGCCAGCACCGCACGAGCCAGGCAATCAGCCGCCGCCGCACCGATCGCGGTGACCAGCTGAGTCTCCGGAGACAATGCGGCCGGCACGCCGGNCTCCGGCGG
    CACCGCTACNGCGCCCGGTGG
    :::::::::::::::Rv128T7.seq:::::::::::::::
    GTGATGGCACGCCACCGCGACACCACCCGGCTGCGCTACNTCGAGCCATACCGGGCGGAGCTACATCGGCTCGGCCGCCCAGTGTTCGGGCCCTCTTTCGAGGTCG
    AGGTCGATACCGATTTGCGCATCCGCANCCGCNCCCTGGACGACAGAACCGTGCCCTACGAGTGCTTGTCGGGCGGGGCCAAAGAACAGCTTGGCATCCTGGCGCG
    ATTGGCCGGCGCGGCGCTGGTCGCCAAGGACGACGCCGTTCCGGTGCTGATCGACGACGCGCTGGGGTTCACCGATCCGGAGCGACTATCAAGATGGGGGAGGTCT
    CTGACACCATCGGCCCCNACGGACATGTGATCGTGCCGACGTGCAGTCCCACCCCG
    Clone Rv129
    :::::::::::::::Rv129SP6.seq:::::::::::::::
    GCGAAAGTCCGTTGGGTGCAATGATGTAGCGCTTCTCCCCATCGAGATAGTGGAGCAACGCAATCCGTGCGGTACGGTTCGGGTCGTACTCGATGTGCGCGACCTT
    GGCGTTGACACCATCTTTGTCATTGCGGCGAAAGTCGATCATCCGGTNNGCGCGCTTATGACCGCCGCCTTTGTGCCGGGTGGTAATCCGGCCATGCGCGTTGCGT
    CCACCGCGACCGTGCAGCGGGCGCACCAGCGACTTCTCCGGGGTTGACCGGGTGATCTCGGCGAAATCAGATACGCTGGCGCCGCGACGACCAGGCGTCGTGGGCT
    TGTNCTTGCGAATTGNCATGTCTAATCANGTCTTTCTCTCACGCTCTCGTCGCCGGGCTAGGCCGCATTGCCCTGCTCCTCCTCATCGCTTCGCTCTGCATCGTCC
    CCGGGCTAAGCCCGTGCCCCGAAA
    :::::::::::::::Rv129T7.seq:::::::::::::::
    GATGGTTCGCGGCACGGTCAACCTGCCACACGGCACTGGTAAGACTGCCCGCGTCGCGGTATTCGCGGTTGGTGAAAAGGCCGATGCTGCCGTTGCCGCGGGGGCG
    GATGTTGTCGGGAGTGACGATCTGATCGAGAGGATTCAGGGCGGCTGGCTGGAATTCGATGCCGCGATCGCGAACACCGGATCAGAATGGCCAAAGTCGGTCGCAT
    CGCTCGGGTGCTGGGTCCGCGCGGCCTGATGCCCAACCCGAAAACCGGCACCGTCACCGCCGACTCCCCATGGCGTCCCGGATATCAAGGGCCGGCAAATCAACTT
    CCCGGTTGATCAGCAAGGCAACCTGCCTCCNCCTCCGG
    Clone Rv130
    :::::::::::::::Rv130SP6.seq:::::::::::::::
    ATACTCAAGCTTCGTCATAAGACCATGGTGCGCTTTCTTTCACCCGTCCAGAGTCGGGGGCATCCGCACCGGCTCGCATCGCATCATCCTCCCACGACGGGCCGCT
    CATCAGCTTGGGCCATTTCAATGTACTTGATACCCCGCGCTGCGGGTAGGCCACTGCGACAATTCAAACACGGTGTCACACGGTGAATAGTGTCGAGATGGGCTCT
    GATCAACCGTCGCAAACCCGGTTTCGCATCAATAGCGGAATCCCACCGGGTTGCATGGAGGCTGCTGACCTTGGAAAACAAAATTTTTTCATTACAACAAAACAAC
    CGCCNCGGAAACTTTGCA
    :::::::::::::::Rv130T7.seq:::::::::::::::
    CGAATTCGGCGTGCACCGCTATGGGTTGCAGCAGCGGCTGGCGCCGCACACCCCACTGGCCCGGGTGTTTTCGCCCCGAAGCCGGATCATGGTGAGCGAAAAGGAG
    ATTCGCCTGTTCGATGCTGGGATTCGCCACCGCGAGGCCATCGACCGATTACTCGCCACCGGGGTGCGAGAGGTGCCGCAGTCCCGCTCCGTCGACGTCTCCGACG
    ATCCATCCGGCTTCCGCCGTCGGGTGGCGGTAGCCGTCGATGAAATCGCTGCCGGCCGCTACCTGCAAGGTGATTCTGTCCCGTTGTGTCGAAGTGCCTTTCGCGA
    TCGACTTTCCGTTGACCTACCGGCTGGGCGTCGGCACAACACCCCGGTGAAGGTCGTTTTTGTTGCAGTTGGGCGGAATCCGTGCTCTGGGTTACAGCCCCGAACT
    CGTCACGGCGGTGCGCGCCGACGGAGTTGTTATCACCGATCCGTTGGCCGTACCGCGCCTTGGGC
    Clone Rv132
    :::::::::::::::Rv132SP6.seq:::::::::::::::
    TCAGACTCCACCCAGCCAGCAGGCAGCGCGAAGCTGAATCCTCCAACCGGGTTGTCGATCCGGACAGGTTGGGGTGCGTTTGGGGCAATGACAGGTGGCGGCGGTG
    CGTTCGGGTCGGCCGGCGGAGGTGCTGCGTTGGATCGCCCGGCTGGCATTCNGCGTGTTGGCGCGGCCGGTGGTGGGGGGCAACACAGGTGTCGGCCGGTGCAGGG
    TGGCGCTGCAGCGGTCGACGGCGGCGAAGCGGCCGTTGTGGGTACCGGGGGCGCTGGCTCCGGATCGGCGTTGGCGGTCGCGGGCACCGCAACGGTCACCAAGCTG
    GCGCTGGCCATCGCCGCGATAGCCAGTGCCGCCAATCGTCCCTTGCGACGTGTCAAGTNGGGGTCCACCTGATGCATGGCCAAAGAACCTACCGTGTTAACGGCNC
    AACNCAAGGACCGCGCCGGTCGCN
    :::::::::::::::Rv132T7seq:::::::::::::::
    TTTCCGCGGTACCCGCTCAACTTTGTGTCNACCCTCAACGGCATTGCCGGCACCTACTACGTNCACTCCAACTACTTCATCCTGACGCCGGAACAAATTGACGCAG
    CGGGTCCGCTGAACAATTCGGTCCGTCCCACGAAAGAACCAGTTTTNCNTCTTTCNCACGGAGAACCTGCCGCTGCTAGAGCCACTGCGATCGGTGCCGATCGTGG
    GGAACCCACTGGCGAACCTGTGTTTCAACCAACACTTAGAGTGTAATTGTAAACCTGGGCTAGGGGAAACCGGCTCTAGTTTTTCCACCNTCTCCGCCCCNTGTTT
    CGAATACTCCGTTCGGGTTGTCCCCAAA
    Clone Rv132
    :::::::::::::::Rv134SP6.seq:::::::::::::::
    GCTTCCGGCTCGTATGTTGTGTGGAATTGTGACCGGATACCAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTAGTTAGGTGACACTATACAATA
    CTCAAGCTTGCCGGCTGGTGGGCCGACCACTTCGATGGCACGACCCGTGAACTGCTGCCCGGCCAATTCTTCTTGGTCGCCCGGACCGATGGACCGCGGCTGGGAT
    TCCAGAAGGTGCCCGATCCCGCCCCTGGGAAAAACCGCGTGCACCTCTACTTCACGACCAACGAC
    :::::::::::::::Rv134T7.seq:::::::::::::::
    CCGATCGACTGATGCGCCGACAACCACGCCCCAACAACTGGAATGAACCGTCGTGACCATCATCAGCACGCGGTTGTAGGCGACTTGCGACATGTTCAACCCGCCG
    TACTCGGACGGAATCTTCAAACCGAAACAGCCCAGCTCGGCCAGGCCTTTCACGTACTCGTCGGGGATCTGGGCACCACGCTCGAGGACGCTGCCGTCCACGGTGT
    CTAGGAATTCCCGCAGTTTGACCAGAAACGCCTCGGTTCGGGCCTCCTCGGCGTCCGACGGCTTGGGAAATGGGTGTATGAGCCCTACGGGAAACCGGCCCACAAA
    GAGTTCTTTGGCGAAGGACGGTTTATCCCAACCACTTTCGCGAGATTCCTCGGCAAGGGCCCGCGCTTGCTCCTCGGTGACCTGAGTTTGCTGTGCCATCGCCGCC
    TCCTCCCTGA
    Clone Rv135
    :::::::::::::::Rv135SP6.seq:::::::::::::::
    TGCATCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACACTATAGAAT
    ACTCAAGCTTTTACGGTGATCGCGCATCACCTGGTTCATGAACTGGAAGCAGCGCAGCGCTTCCTTTTCGGCCGCAACATGAGCCAGCCTCTCGTCGGCGGTCGGG
    TGCAGGTGCTCGGGCAGCTCGGCCGCGACAGCCGCCTGACCCTGAAACCAGCTTCCATATCCCGCGACGAACGACGCCAGTCCGCTACGTAACCCCTCCGCGACTG
    TCCATGGACAACAGCGCGTTCTCCACCGACCGGGCCCGGGTGTGGGGTGTTTCGGCGACCGGCAGCCAGGTGGTCCACACTGCCGACGGGCGCCGCGAGCCGTTCA
    CCGACCAGGCCGCCGAGCAAGTCCGCCCGATCGCATACTCC
    :::::::::::::::Rv135T7.seq:::::::::::::::
    GGGGGCGCTGCTGGTATAGTCGCTGACCGGTGCAGGTTTCGACAATGTGGTGCCGGTTCGGCGGCTACGTGCCATCGAGACACTGGCCAGGCTATCGCACCCGGTT
    ATCGGCTACGAGCAAATCGCGGTATGCGTTCTTGAGCATGAGTCGGCGACCGTCGTCATGGTCGACACCCACGACGGAAAGACGCAGATCGCCGTCAAGCATGTGT
    GCCGCGGATTATCAGGACTGACCTCCTGGCTGACCGGCATGTTTGGTCGCGATGCCTGGCGCCCGGCCGGCGTGGTCGTGGTCGGCTCGGATAGGCAGGTCAGCGA
    ATTCTCGTGGCAGCTCGAAAGGGTCCTGCCGGTGCCGGTCTTTGCGCAAACGATGGCGCAGGTTACGGTCGCGCGGGGTGCGGCCTGGCGGCGGCCAGAGCAGGAG
    TTCACCGATGCGCAGCTAGTGGCGACAGCGTCAGCCAAC
    Clone Rv136
    :::::::::::::::Rv136SP6.seq:::::::::::::::
    TGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACACTATAGAAT
    ACTCAAGCTTCCGTACAGGTCGCCTCCAACACGGCGGGGAAGCGACACCAGCCTACCGAGCTTGGAGTCCAGGACGCCAGCGGCGGCGTCGGTCTGCGTCGTGGTG
    CCGCCGGGGTGGCGTTGGCTGGCAACGATCTCCACCCAGCCGGTCGGGTTACCCACGATCTCGGCATAGACGCGGGCCGAGGCCGGTGCGATACCGTATTGCGTCA
    ATTGGGACGCGGTTGTGCATTCGGCTAGCTCGGTTGCCACACCCGTCAGGGGTTCGACGTTGGCGGGTTCGGCGGGCCCCAGCACCGCTGTCACCATGCCCGCCAA
    GCCGACCTGCGGCGCCACCAACT
    :::::::::::::::Rv136T7.seq:::::::::::::::
    CGGCATGACCACCGACAGGCCCGACTGGTCGTACCACTCGAACGCCGGGGTGTTGATGTCCCAGCCGCTGAAGTCGTCCTGCGCGCGCGGCCGTCGAAGCAGGTAC
    AGGGCGGGCGAGTTGGCACCACCACTTTGGAATTGGACCTTGATGTCACGGCCCATCGACGGCGACGGCACCTGCAGGTACTCCACCGGCAAGCCCGGCCGGGAAA
    ATGCCCCCGCGGTCGCCGTGCCACCGACGGCGCCGACCAGACCCGACACTAGGGCCGCGCCGACGGCCCGGACCACGAGTCGACGCGACATACCCGTGACGGCGCC
    ACGAACCCTGTCAACAAGCTGCATTCTTGCTTCCCTCATCCTCATCTCAACGCATCCATGCATGTTTGGGCGCATCCTGAATTANGTCAGACTGCAGGCGCTGGGC
    CGGCAGTGCTCGTGTATCAACCACAACTTCGGGCGT
    Clone Rv137
    :::::::::::::::Rv137SP6.seq:::::::::::::::
    TTCCAACCCTAATTGGCTTTCGGCCCCATCCGTGAGGACGGGGTGCGGGTGCTCAACAACAACGTCGTCCGCGGGACACACCTCTATGCTGCGGCCATGGACGCGG
    TCCAACGCAAGCAGCTGATCGAGCTACAACCCCGCGCGGAACGCTTCCGCCGCGGGCGTGACCGCATCCCGTTGACCGGGCGGATCGCGGTGATCGTCGATGACGG
    CATCGCCACCGGAGCGACGGCCAAGGCGGCGTGCCACGTCGCCCGGGCGCACGGTGCGGACAAGGTGGTGCTGGCGGTCCGGATCGGCCCAAACGACATCGTGGCG
    AGATTCGCCGGGTACGCCGATGAGGTGGTGTGTCTGGCGACGCCGGCGTTGTTCTTCGCCCTCGGGCAGGGTTACCGCAACTTCAC
    :::::::::::::::Rv137T7.seq:::::::::::::::
    CAGGCATGCAAGCTTTCCGCCGATACCCGCCATGTCGCGCACATCCAGGACTTCTGGGGGGATCCGCTGACAGCGGCGGGATCCCAAAGTGCGGATGATCGGGCCG
    CCTACGTCGTGGTGTACCTCGTCGGTAACAACGAAACCGAAGCGTATGACTCGGTCCACGCGGTGCGGCACATGGTGGACACCACACCGCCACCGCACGGGGTGAA
    GGCCTATGTCACCGGTCCGGCAGCACTCAATGCCGACCAGCCGAGGCCGGAGACAAAAGTATCGCTAAGGTCACCGCCGATCAACNAGCATGGTGATCGCAGCAAT
    GTTGCTAGTGATCTATCGCTCCGTAATTACCGCGGTTCTCGTCTTGATCATGGTCGGCATCGACTCGGCCAATCCGCGGATTCATCGCCTTGCTCGCCGAACACAA
    CATTTTCACCTTTCACATTTGCACCAACCTGCTCTTCTCAT
    Clone Rv138
    :::::::::::::::Rv138SP6.seq:::::::::::::::
    CACTACTCAAGCTCTCTCNTCATTACCACCCCTGTAATTTGGGATGGGCAAAAGGCGAAGCACCGCTTGGCCACNAACGCCGGGACGGGACAATCTCGGGCGGCTA
    TGGCTTCTCCCGGAAAGGCCCCAACGTACGGCGTTTCAACACGTCGCGTCGCCCTCCGACCGCGAACATTCGGGGATTGGCACCAACCTGNTACCACCCTGGCCGG
    GCGATGATCTGCAGCGTCGCCGCGGGTAGTCCCCGCCCGGGCGGCTACAGTCTGAAACCCCGATGACCATCGATGTGTGGATGCAGCATCCGACGCAACGGTTCCT
    ACACGGCGGATATGTTCTCCTCGCTGCGCCGGTGGACCGGTGGGTATATCCCCTGAAACCGACATCCCN
    :::::::::::::::Rv138T7.seq:::::::::::::::
    CAGGCATGCAAGCTTTCGTCAGTTCATTGCGCCAGCAGACCAACAAGAGCATCGGGACATACGGAGTCAACTACCCGGCCAACGGTGATTTCTTGGCCGCCGCTGA
    CGGCGCGAACGACGCCAGCGACCACATTCAGCAGATGGCCAGCGCGTGCCGGGCCACGAGGTTGGTGCTCGGCGGCTACTCCCAGGGTGCGGGCGTGATCGACATC
    GTCACCGCCGCACCACTGCCCGGCCTCGGGTTCACGCAGCCGTTGCCGCCCGCAGCGGACGATCACATCGCCGCGATCGCCCTGTTCGGGAATCCCTCGGGCCGCG
    CTGGCGGGCTGATGAGCGCCCTGACCCCTCAATTCGGGTCCAAGAACATCAACCTCTGCAACAACGGCGACCCATTTGTTCGGACGGCAACCGGTGGCAACGCACC
    TAAGCTACTTGCCCGGGATGA
    Clone Rv139
    :::::::::::::::Rv139SP6.seq:::::::::::::::
    GTTTATGCACTGGTTAGGTGTTTCCATGAGTTTCATTCTGAACATCCTTTAATCATTGCTTTGCGTTTTTTTATTAAATCTTGCAATTTACTGCAAAGCAACAACA
    AAATCGCAAAGTCATCAAAAAACCGCAAAGTTGTTTAAAATAAGAGCAACACGTACACAAGGAGATAAGAAGAGCACATACCTCAGTCACTTATTATCACTAGCGC
    CCGCCGCAGCCGTGTAACCGAGCATAGCGAGCGAACTGGCGAGGAAGCAAAGAAGAACTGTTCTGTCAGATAGCTCTTACGCTCAGCGCAAGAAGAAATATCCACC
    GTGGGGAAAAACTCCAGGTAGAGGTAC
    Clone Rv13
    :::::::::::::::Rv13SP6.seq:::::::::::::::
    ATACTCAAGCTTGGGTGTAGCCGATCACCGGAAGTCNCATGATCAGCCACGTTCCGCGCCGCCCGGCATACGGTGGTGTACCGATCTCCGCGTCATACACCCGCGG
    GTAATCGCCGACGGTGCCGGTTCGCGAGCCGAA
    :::::::::::::::Rv13T7.seq:::::::::::::::
    AGCTTTATCGAAAGCGCGAACAGCTCGCGGCGGCCCACGACGTGCTGCGTCGGATTGCCGGCGGCGAGATCAATTCCAGGCAGCTCCCGGACAATGCGGCTCTGCT
    GGCCCGCAACGAAGGACTCGAGGTCACCCCGGTGCCCGGGGTCGTGGTGCACCTGCCGATCGCACAGGTTGGCCCACAACCGGCCGCTTGATGCCCGGTCGGCAAG
    CCCGGCAGTTGCCAAACCCATCGTGATCAGGCTCGGCTCGCGAGTTCGGCGAAGAAATGGTTCGCCTGATCACCTACCATCGGCCA
    Clone Rv140
    :::::::::::::::Rv140SP6.seq:::::::::::::::
    TCAACACGCCGCCAGCCACCACGCGCGGGTCGGGCGCCGGGCCCGGGCCTCCAGGCTNCTCCGCTCGGTGATGGCACGCCACCGCGACACCACCCGGCTGCGCTAC
    GTCGAGCCATACCGGGCGGAGCTACATCGGCCCGGCCGCCCAGTGTTCGGGCCCTCTCGCCCAGGTCGAGGTCGACACCGATTTGCGCATCCGCAGCCGCACCCTG
    CGACGACAGAACCGCGGCCCTACCCACTGCTTGTCGGGCGGGGGCCAAAGAACCAGCTTGACATCCTGCCACAATTGGCCGGCGCCCG
    :::::::::::::::Rv140T7.seq:::::::::::::::
    CAGGCATGCAAGCTTCACGTCCGTACGGCTCGGGTACGCTTCGGTCGCAGTGTGCGAGTGATAGATGACGACCGGGACCTCGTCGGCATCTTCCATAGCCCGCCAC
    ACCTTCAGTTGCTCACCGGAATCCAACCGGTAGAAGGTCGGCCAGCGCTCGGCATTGGTCATCGGGATATGCCGCTCGGGACGGTCAGAGCCCTCGGGTCCGGCCA
    GCACTCCGCAGGCTTCGTCGGGGTGGTCGCGACGCGCATGGGCCACCATCGCATTCACCAGGTCTGCGCGAATCACCAGCACGTAGACGGTTCCTTTCCTAAGCAA
    CAC
    Clone Rv141
    :::::::::::::::Rv141SP6.seq:::::::::::::::
    AATATTCAAGCTTTCGGCGGAAACGGACNCCTTGCGAACATTGATAACAAAATAGAAATCATTGATGGTTTGAGTCACCAGGCCGATCAAGCCTTCGCCGAGCCAA
    ATTCCAATCAAGAGGCCCAAGCCCGTACCAATCAGCCCGGCAACGAGGGATTCCGTCNTTATCAGCCNAAATAACTGCTCTCGGGTACCACCCAAACAGCGCAATA
    TGGCGAAAAACGGTCGCCGTTGCACAACATTAAATGTCTCGGTATTGTTGATTAAAAAGATACCCACCACCAGGGCAATCCAACTGAGAGGGGTTAAATTGACCGT
    AAAAACCTCCCGTCATCTGTTT
    :::::::::::::::Rv141T7.seq:::::::::::::::
    CAGGCATGCAAGCTTGCTGCATCTTCCTGTGACTGCTCCCGAAACCTGGGGGTGTGCCTGCTGTGTATGCACGGCATACGGACATCCTTCCCCTGATACCCGCGGT
    CGAACCAGCCACGTGTCCATCATCAGGGGTCAACCCCGGCCAAGGGCGACGGCACGCCAAGTTCGCCGACCGTTAACCTAGTGCTGTTAGCTTCATTTGCTGCGAG
    CAAAACAGCTGGTCGGCCGTTAGGAACTGAATTGAAACTCAACCGATTTGGTGCCGCCGTAAGTGTCCTGTCTGCGGGTGCGCTGGTGTTGTCCGCGTGTGGTAAC
    GACGACAATGTGACCGGGGGAGGTGCAACCACTGGCCAGGCGTCCGCGAAAGTCCATTGCNGGGGGAAGAAGACAC
    Clone Rv142
    :::::::::::::::Rv142IS1081.seq:::::::::::::::
    GAAAGTGCCCCAAGGTGTTGGTGAAACTCGCTGGACGGTCCCCAGGATGTTGGCAGCACATTCACCGGACATGACCGGAGCAAGACCGGACATCCTCCCATACCGT
    CGTCGCCGTGTACATCCGTAGCCCGTCCTGGCAGGTGCTGGGTTGAACAAAATCAGCCCAACACCTGCCACGACGAAGAAGCGGGTTGCGCTGGCATGTCTTGTCG
    GCTCGGCGATCGAATTCTACGAATTCCTTATCTACGGGACCGCTGCGGCGCTGGTGTTTCCCACCGTGTTCTTCCCACACCTGGATCCCACGGTGGCCGCCGTGGC
    CTCCAAGGGGACATTTGCTGTGGCGTTCCTATCCCGGCCGTTCGGCGCGGCCGTCTTTGGATACTTTGGAGACCGCCTCGGCCGCCAGAAGACCCTGGTCGCCACA
    CTGTTGATCATGGGCCTGGCAACCGTGACTGTTGGGCTGGTTCCACGACAGTGGCCATCGCGC
    :::::::::::::::Rv142SP6.seq:::::::::::::::
    ATATTCAAGCTTTGTCACACCAAGTGTTCCGACCAANCGCTCCATCCGGCGAGTGGATACTCCCAGCAGGTAGCAGGTCGCCACCACGCTGGTCAGTGCGCGTTCA
    TCTCGCTTGCGGCGCTGCAGCAGCCAGTCCGGGAAATAGCTGCCCTGGCGCAGCTTGGGGATCGCGACGTCGATGGTTGCGGCACGGGTGTCGAAATCACGGTGGC
    GGTAGCCGTTGCGCTGATTGGACCGCTCATCGCTGCGTTCGCGGTAGCCCNCCCCGCACAGGGCGTCGGCTTCAGCCCCCATCCAAGGCGGCGATGAACGTCGAGA
    GCAGCCCGCGCAGCAAATCCGGGCTCGCCTGTGCGAGTTGGTCAGCCAGAAGCTGCTCGGTGTCATAAGATGAGAAGAGGTCAGTGCGTCCTTTCCTTCG
    :::::::::::::::Rv142T7.seq:::::::::::::::
    CAGGCATGCAAGCTTTTTGAGCGTCTCGCGGGGCAGCTTCGCCGGCAATTCTACTAGCGAGAAGTCTGGCCCGATACGGATCTGACCGAAGTCGCTGCGGTGCAGC
    CCACCCTCATTGGCGATGGCGCCGACGATGGCGCCTGGACCGATCTTGTGCCGCTTGCCGACGGCGACGCGGTAGGTGGTCAAGTCCGGTCTACGCTTGGGGCTTT
    GCGGACGGTCCCGACGCTGGTCGCGGTTGCGCCGCCAAAGCGGCGGGTCGGGTGCCATCATGAATGCCTCACCGCCGCCGCACTGCACGGGCAGTGCCCGGGCGAT
    GTCAGCCATCGGGACATCATGCTCGCGTTCATACTCCTCGACCAGTCCGCGGAACAGCTCCATTCCCGGACCGCCCAACGC
    Clone Rv143
    :::::::::::::::Rv143SP6.seq:::::::::::::::
    ATACTCAAGCTTTTGGCTGGGTCGCCTTCCAATTCAGCGTGCACCGCTATGGGTTGCAGCAGCGGCTGGCNCCGCACACCCCACTGGCCCGGGTGTTTTCGCCCCG
    AACCCGGATCATGGTGAGCGAAAAGGAGATTCNCCTGTTCGATGCTGGGATTCGCCACCGCGAGGCCATCGACCGATTACTCGCCACCGGGGTGCGAGAGGTGCCG
    CAGTCCCGCTCCGTCGACGTCTCCGACGATCCATCCGGCTTCCGCCGTCGGGTGGCGGTAGCCGTCGATGAAATCGCTGCCGGCCGCTACCACAAGGTGATTCTGT
    CCCGTTGTGTCCAAGTGCCTTTCGCGATCGACTTTCCGTTGACCTACCGGCTGGGGCGTCGGCACAACAGCCCGGTGAGGTCGTTTTTGTTGCAGTTGGGCGGAAT
    CCGTGCTCTGGGTTACAGCCCCGAACTCGTCACGGCGGTGCGCCGCCGAC
    :::::::::::::::Rv143T7.seq:::::::::::::::
    CAGGCATGCAAGCTTCAACCTATTGACGCATTGTGCGAACTGACGGCGCCCGCGCATGGCCAATCCGGAAGACCATCATTGGCCAGTGGCCGGGCGCTAACAGGTT
    CCAGCCCCCCACCAGTGCCGCTCGAACATGCGGTGCAACCCATTCGCAGGCCGGCAGGGAAAGCACCGCGGAAGCCGCAAAGGGCTGCAGTTCCGCGCCCAATAGT
    GTCGTCCGCAACCAGATGCGCTCGAAAACCGCCGCCGGCAGTCAGCGCACCCGACGCGAGGTCGAGAGACGTCGTCAGCGCGCCCACATGGGGTGCCAATCGGCAC
    GGCAGGTAGGCCGCGCGCAACCCCAACGCGTGGTGCATGCCACGGTCCGCAGGAGGCCACCACCC
    Clone Rv144
    :::::::::::::::Rv144SP6.seq:::::::::::::::
    ATACTCAAGCTTCCCGGCCGCAGGTGACGGCGCGGCCTAGCGCCACTTGATGCCGCACCCGATCGACGGNCGTTGGTCGGGGTTGACTGGGGGCCCGGCGAGCAGG
    GCGTCAACCGCGGCCCGGACGTCGGCGGCCGTCACCGGTCGGCCATTGCCCGGGCGGGAGTCGTCGAGCTGACCACGGTAGACAAGTCGGCGCTGGCCGTCGAAGA
    CAAACGTGTCGGGTGTGCAGGCCGCGGAGAAGGCGCMGGCGACGTCTCGGGTTTCGTCGTAGAGATACGGGAACGTCCAGCCGTGGCGGCGGGCCTCGGCGACCAT
    CTGATCGGGCCCGTCCTGCGGGTAGGTGACCACGTCCTTACTGGAGATACCGACCATCGGGACCCTTTGATCGGCGAGGTCCCGGCCGACCGTGGCCAATCCGGCG
    GCGACGTGTCGCCCGTACCGGCCAGTGGTTC
    :::::::::::::::Rv144T7.seq:::::::::::::::
    CAGGCATGCAAGCTTTANCANCATCAACCCCGCCCCGCACCAGCACCGACACGATGTCGATGCCATCGAGGTGAATGTCGAACTGGCNCAAACCATCTGGCGACCG
    CGACCACCGGCAACATGGGTACCGGCGATTTCCGGTGCCAATGCCGACCCGACGGGCCGCTCTCACCGCAGGTGACCTCGATCACCGAGACCAGCCGGCCGTTATA
    CTCACGCACCCCTACCGTGTCACGCCCAAAACGGCGCTGGTGGTCGATTGCCGGAGTGCACCCCGCACCCAGTGTCGTGCCCGGATCCGCCGACCAATCCCGCACC
    CACGTCGCCAAACCCGAAATCACCGTGATGCCGTGGTAACTGACCACCGACAGTAACGTCACTACGGCCGCCACGCCGACGCCGAACCACCACGCACATGATGATC
    GGCTG
    Clone Rv145
    :::::::::::::::Rv145SP6.seq:::::::::::::::
    ATATTCAACCTTGCACACATTGACGATACCTTGGTCACGAGACCCCAAAAGCTGGCCTCCACCGCGCGCCGGGGACCACGGTCATACCTTGANNCNGCTTTCGATC
    GTTGATGCTGCGTCTTGGTCCGCGGAAACCGCAGGCTGGCATATGCACGTGGGCGCACTGGCGATCTGCGATCCCCACCGATTCGCCCGAATACAGCTTTCAGCGG
    CTCCCCAAGTTGATCATCGACCGGCTGCCGGATATCCCGCACTTGCGGTGGCGGGTCACCGGCGCCCCGCTCGGACTGGACCGGCCGTGGTTCGTCGAGGACCACG
    AAC
    :::::::::::::::Rv145T7.seq:::::::::::::::
    CAGGCATGCAAGCTTCATGCCCGCGGCATGATAGCCACATGCACGCAATCGAACTCAGCGAAACCGGCGGGCCAGGCGTCTTACGCCACCTCACCAGCGCGCAACC
    TCAACCCGGCCACGGAGACCTCCTGATC
    Clone Rv146
    :::::::::::::::Rv146SP6.seq:::::::::::::::
    ATACTCAAGCTTGATTTTGATCATCATGATGATCATCACCCGAATTGTGGTAGCCGCAGTGGTTATCGTGGGTACCGTCGTGCTTTCCATGGGCGCCTCTTTCGGG
    CTTTCCGTATTGGTCTGGCAGGACATTCTGGGTATCGAGTTGTACTGGATGGTGTTGGCGATGTCGGTGATCCTGCTCNTGGCGGTGGGATCCGACTACAATCTGC
    TGCTGATTTCCCGGTTGAAAGAGGAAATTGGGGCCGGATTGAACACCGGAATTATCCGTGCCATGGCTGGTACCGGGGGAGTGGTGACGGCTGCCGGCATGGTGTT
    CGCCGTTACCATGTCGTTGTTTGTGTTCAGCGATTTGCGAATTATTGGTCAGATCGGTACCACCATCGGCTGGGCTTGCTGTTCGACAGCCTCGTCGTGCCTCGT
    TCATGAAACCGTCCATTGCTGCCCTGCTGGGACCTGGTTCTGGTGGCCGCTACGGGTGCGCCCGCGCCCGGCAGTCAAATCTTCCGCCG
    :::::::::::::::Rv146T7.seq:::::::::::::::
    CAGGCATGCAAGCTTGGCGTGCCGTTCCAACCCGAATTGGCTTTCGGCGCCATCGGTGAGGACGGCGTGCGGGTGCTCAACGACGACGTCGTCCGCGGGACACACC
    TCGATGCTGCCGCCATGGACGCGGTCGAACGCAAGCAGCTGATCGAGCTACAACGCCGCGCGGAACGCTTCCGCCGCGGGCGTGACCGCATCCCGTTGACCGGGCG
    GATCGCGGTGATCGTCGATGACGGCATCGCCACCGGAGCGACGGCCAAGGCGGCGTGCCAGGTCGCCCGGGCGCACGGTGCGGACAACTTGGTGCTGGCGGTCCCC
    ATCGGCCCAGACGACATCGTGGCGAGA
    Clone Rv147
    :::::::::::::::Rv147SP6.seq:::::::::::::::
    ATACTCAAGCTTTTACGGTGATCGCGCATCACCTGGTTCATGAACTGGAAGCAGCGCAGCGCTTCCTTTTCGGCCGCAACATGAGCCAGCCTCTCGTCGGCGGTCG
    GGTGCAGGTGCTCGGGCAGCTCGGCCGCGACAGCCGCCTGACCCTGAAACCAGCTTCCATATCCCGCGACGAACGACGCCAGTCCGCTACGTAACCCCTCCGCGAC
    TGTCCATGGACAACAGCGCGTTCTCCACCGACCGGGCCCGGGTGTGGGGTGTTTCGGCGACCGGCAGCCANGTGGTCCACACTGCCGAAG
    :::::::::::::::Rv147T7.seq:::::::::::::::
    TAGTCGCTGACCGGTGCAGGTTTCGACNATGTGGTGCCGGTTCGGCGGCTACGTGCCATCGAGACACTGGCGCAGGCTATCGCACCCGTTATCGGCTACGAGCAAA
    TCGCGGTATGCGTTCTTGAGCATGAGTCGGCGACCGTCGTCATGGTCGACACCCACGACGGAAAGACGCAGATCGCCGTCTANCNTGTGTGCCGCGGATTATCAGG
    ACTGACCTCCTGGCTGACCGGCATGTTTGGTCGCGATGCCTGGCGCCCGGCCGGCGTGGTCGTGGTCGGCTCGG
    Clone Rv148
    :::::::::::::::Rv148SP6.seq:::::::::::::::
    ATACTCAAGCTTTCCGCCGATACCCGCCATGTCGCGCACATCCAGAACTTCTGGGGGGATCCGCTGACAGCGGCGGGATCCCAAAGTGCGGATGATCGGGCCGCCT
    ACGTCGTGGTGTACCTCGTCGGTAACAACGAAACCGAAGCGTATGACTCGGTCCACGCGGTGCGGCACATGGTGGACACCACACCGCCACCGCACGGGGTGAAGGC
    CTATGTCACCGGTCCGGCAGCACTCAATGCCGACCAGGCCGAGGCCGGAGACAAAAGTATCGCTAAGGTCACCGCGATCACGAGCATGGTGATCGCAGCAATGTTG
    CTAGTGATCTATCGCCCCGTAATTACCGCGGTTCTCGTCTTGATCATGGTCGGCATCGACCTCGGCGCAATCCGCGGATTCNTCGCCTTGCTCGCCGACCACAACA
    TTTTCAGCCTTTCAACATTTGCGACAACCTGCTCGTTCTCATGGCGATTGCNGCGAAC
    :::::::::::::::Rv148T7.seq:::::::::::::::
    CAGGCATGCAAGCTTGGCGTGCCGTTCCAACCCGAATTGGCTTTCGGCGCCATCGGTGAGGACGGCGTGCGGGTGCTCAACGACGACGTCGTCCGCTGGACACACC
    TCGATGCTGCCGCCATGGACGCGGTCGAACGCAAGCAGCTGATCGAGCTACAACGCCGCGCGGAACGTTCCGCCGCGGGGCGTGACCGCATCCCGTTGACCGGGCG
    GATCGCGGTGATCGTCGATGCGGCATCGCCACCGGAGCGACCGGCCAAGGCGGCGTGCCAGGTCGCCCGGGCGCACGGTGCGGACAAGGTGGTGCTGGCGGTCCCG
    ATCGGCCCAGACGACATCGTGGCGAGATTCGCCGGGTACGCCGATGAAGTGGTGTTGTTGGCGACCCGGCGTTGTT
    Clone Rv149
    :::::::::::::::Rv149SP6.seq:::::::::::::::
    ATACTCAAGCTTTGGCATTGTGCACATTTTCCACCCGTGCTCTATTAATGCTGAGCCGCTAATTGTGACCCCAGTCGGGAAACACGCGGAGCACCAAATTCACCGC
    AGCGGCCGGGGCGGTTCAACTCACCATGGATCGCTCTCGTCGTCTGGTGCTGGACAATCGTCGCTGTAGCGCGTCGCGAACACCTCAGCTTCTGCTGCCGCGGCTT
    CTTCCGGCGATGGTAACCCCCAGGTTTCGCCCACGGTCTTACGTAGCAGTGCGACGCGGTGTTCATCTGCATCGACCTGTTGACTCATCCTGTCAAGGATGAAGGC
    GTACTGGGCCGACTGCGCCTTCTGCCGCGCCAGGTCGGCAATCACCAGGATCTCAGAAACGAGCTGCGACTCACTCTTCCAGGCCACCCTGGCCGAAAGCTCGACA
    TGGTCAATCCGGCCG
    :::::::::::::::Rv149T7.seq:::::::::::::::
    CAGGCATGCAAGCTTGCGGGCCGGAGTGGTTTCGACGGCCGCTCGCTTCTCGGCATCGGTTTGGGCTGTCACCAGCAGTTGGTAGTTCTTCACGTACTGTTGTTCG
    AGCGTCGAGCCGCCGCGCGTGTCGAGGTCGCCGGACGCGTATCCCGCCAGGCCGGTCAGGGTGCCCTTCCAGTCCACGCCGCTGTGGTCGGCGAACCGCTTATCTT
    CAATCGAGACGATCGCCAGCTTCATCGTGTTGGCGATCTTGTCCGAGGGCACCTCGAACCGGCGCTGCGAGTACAGCCACGCGATCGTGTTGCCCTTCGCGTCGAC
    CATCGTCGATACCGCAGGCACTTGCCCCTC
    Clone Rv14
    :::::::::::::::Rv14SP6.seq:::::::::::::::
    ATACTCAAGCTTCCCGGCGGCCAGTACCGAAAGCGCGAACAGCTGGCGGCAGCCCACGACGTGCTGCGTCGGATTGCCGGCGGCGAAATCAATTCCAGGCAGCTCC
    CGGACAATGCGGCTCTGCTGGCCCGCAACGAAGGACTCGAGGTCACCCCGGTGCCCGGGGTCGTGGTGCACCTGCCGATCGCACAGGTTGGCCCACAACCGGCCGC
    TTGATGCCCGGTCGGCAAGCCCGGCAGTTGCCAAACCCAGCGTGATCAGGCTCGGCTCGCGAGTTCGGCGAAGAAGTGGCTCGCCTGATCACCTACCATCGGCCAG
    GATCTGCGTGTCATCACAACGCTCGCCAAGGAGGTTGTTGTGGTGCTATCGACGGCCTTTAGCCAGATGTTCGGAATCGACTATCCGATAGTGTCCGCGCCAATGG
    ACTTGATCGCCG
    :::::::::::::::Rv14T7.seq:::::::::::::::
    AGCTTCGGTGTAGCCGATCACCGGAAGCCGCATGATCAGCCACGTTTCGCGCCGCCCGGCATACGGCGGCGTACCGATCTCCGCGTCATACACCCGCGGGTAATCG
    CCGACGGTGCCGGTTCGCGAGCCGAAGGTGACGACGCTGATTGAATCGAGTTCCAGGTCCAGCGGGTGGCGCAGCAACGGCGCGAGCTCAACGACGTCAATCACGT
    TGTCGCTTTCTACGGTCACCGACCCGGTGACCGTAGTCGCCCGGTGCGCTCGGCCGAGAAGTTGCACCGCCACCACCGCGACACCGTCTTGCACGCGGACGCCACC
    CCCGGATCGGTTGTTGGCCAAGGTAATTGGGTCATTCCATTTGACGGGACGCCGACCCCGCAGCCCCAGTACCGCCCACGACCACGCCGGCTGACCCACCACTGTA
    CGAACACCAAGGCGACGCCGA
    Clone Rv15
    :::::::::::::::Rv150SP6.seq:::::::::::::::
    ATACTCAAGCTTCGGTGGCTTCGCCCGCCCTGCCGGGTGGACTTCATGACAACGCGGGGGCGATTACCCCCGCTACCGCCAGCAGCATGACGGCGGTACCTAACAC
    CGCCCGGATGCCTCGCACGTGCCTCGATGTGCTCACGGAATCGCCCCGGCACCGCGATCTCGAGGATCACCAGCGTTACCCCCGGCAGCGCGACACCGACAATTCC
    GTACACCGCCACGCCGATCCGGCCCTGGGCCAGCTGATTGGAGCTGGCG
    :::::::::::::::Rv150T7.seq:::::::::::::::
    CAGGCATGCAAGCTTCCACATGTACGGATCCACGAACATCCCGTTGAACTGACAGGTGCGGCCCGGCTCGATCAGGCCGGCCACTTGTTCTACGCGGTTACCGAAG
    ATCTCTTCGGTGACCTGCCCGCCGCCGGCCAGCTCGGCCCAGTGCCCGGCGTTGGCCGCCGCGGCGACGATCTTGGCGTCCACGGTGGTCCGGGTCTTGCCCGCTA
    GCACGATCCGCGAGTCGGCCGGTCACCCGGGT
    Clone Rv151
    :::::::::::::::Rv151SP6.seq:::::::::::::::
    ATACTCAAGCTTTCCAAGTCCCAAGTGTCGATCATGGCCAAAGAGCTCGACAAAGCCGTAGAGGCGTTTCGGACCCGCCCGCTCGATGCCGGCCCGTATACCTTCC
    TCGCCGCCGACGCCCTGGTGCTCAAGGTGCGCGAGGCAGGCCGCGTCGTCGGGGTGCACACCTTGATCGCCACCGGCGTCAACGCCGAGGGCTACCGAAAGATCCT
    GGGCATCCAGGTCACCTCCGCCGAAGACGGGGCCGGCTGGCTGGCGTTCTTCCGCGACCTGGTCGCCCGCGGCCTGTCCGGGGTCGCGCTGGTCACCAGCGACGCC
    CACGCCGGCCTGGTGGCCGCGATCGGGGCCACCCTGCCCGCAGCGGCCTGGCAGCGCT
    :::::::::::::::Rv151T7.seq:::::::::::::::
    CAGGCATGCAAGCTTCACACGTAGGCGCCGTCGATAAATGACTCCGCCGCGCTTCGCACATCCTCGTAGCGATCCTTGGCGAGCAGGTCAACCGGGCGCTGCCCGT
    CGAGGAGCCGGTTTTTGGCGTGCAGCCACTGGCCGACACCTCGGGGGGTAAGCGAATCCGAGAGCAGGAGGACGAGGTCACGAAGCTGCGCCAGCCGGTCGTACCG
    CTCAGGGCGGATGTCGCCGGTCCGCCACCCGCGTACCGCCCGATCGGACACCTGTATGACCGCGGCGACGTC
    Clone Rv152
    :::::::::::::::Rv152SP6.seq:::::::::::::::
    CGCGGCGGCGCATTACCCCCGCTACCGTCAGCAGCTTGACGGCGGTAGCGAACACCGCCGGATGCAGCGCAGGTGCGTCTATGTGCACACGGAATCGCCCCGGCAC
    CGCGATCTCGAGGATCACCAGTGCCCGCCCCCTG
    :::::::::::::::Rv152T7.seq:::::::::::::::
    GGGATCGAGGAACAGCGCGTTGAACTGATAGGTGCGGCCCGGCTCGAGCAGGCCGGCCATTTGTTCGATGCGGTTACCGAAGATCTCTTCGGTGACCTGCCCGCCG
    CCGGCCAGCTCGGCCCAGTGCCCGGCGTTGGCCGCCGCGGCGACGATCTTGGCGTCCACGGTGGTCGGGGTCATGCCCGCGAGCAGGATCGGCGAGCGGCCGGTCA
    GCCGGGTGAACTTCGTCGAGAGCTTGACCCTGCCGTCGGGGAGGCGAACCACGGTCGGTGCGTATCTCGACCAGGCCCGGGCAACCTCGGGGGTGGCGCCGACGGT
    GAACAGGTTGCGCTGGCCACCGCGGGTAGCCGCCGGCACTATGCCGATGCCCAGGCCGCGGATCACCGGTGCGGTCAGTCGGGTCAGGATGTCGCCCGGCCCCAGG
    TCGAAGATCCAGCGGGCGCCGGCCGCGTGGACACNGGTGATCTCGTCCACCATCGACTTTCTGATCA
    Clone Rv153
    :::::::::::::::Rv153SP6.seq:::::::::::::::
    TAACTCAAGGCTTGCGTTGAGGCCCCAGGCCCATCGACGGTTTGGCGGCCTTAAATGCACTGAGGTCGTCAATTGACCCCACAGCGGAAATGCCGACTATTCGCAG
    GCCTCCTTCGCCTTGGCTGCCGGAGAGGGGCTCCGCGGGAACCGCATGCAGGTATATGACCTCGGTTTCTCGGGTGCTACCGCGTGCCTTGTCGAGGATGAACTCG
    GCGTTGGAATTGTCCAGCCGGCCCAATTCATCGAGCGCAGATTCGTACACATGGCCGGCGGCGACATACGCTTCACCGTGGATCTGCTCCACACGGACCGCCCTGT
    CGGGATCCTGCTCACGGGTAAAGGAACTTACNTGGCNCTCGGTGCC
    :::::::::::::::Rv153T7.seq:::::::::::::::
    CCTTCTGCGCCACCCACACCGTCAACGCCCGCGAAGTCGACGTCGTCCAGGCCATCGGCGGCCTCACGGATGGATTCGGCGCGGACGTGGTGATCGACGCCGTCGG
    CCGACCGGAAACCTACCAGCAGGCCTTCTACGCCCGCGATCTCGCCGGAACCGTTGTGCTGGTGGGTGTGCCGACGCCCGACATGCGCCTGGACATGCCGCTGGTC
    GACTTCTTCTCTCACGGCGGTGCGCTGAAGTCGTCGTGGTACGGCGATTGCCTGCCCGAAAGCGACTTCCCCACGCTGATCGACCTTGACCTGCATGGCCGGCTGC
    CGCTGCAGCGGTTCGTTTCCGAACGCATCGGGCTCGAAGACGTCGAGGAGGCGTTCCACAAGATGCATGGCGGCAAGGTATTGCGTTCGGTGGTGATGTTGTGATG
    GCCGCCATCGAGCGCGTCTCACCCCACGG
    Clone Rv154
    :::::::::::::::Rv154SP6.seq:::::::::::::::
    ATACTCAAGCTTGATTTTGATCATCATGATGATCATCACCCGAAGTGTGGTAGCCGCAGTGGTTATCGTGGGTACCGTCGTGCTTTCCATGGGCGCCTCTTTCGGG
    CTTTCCGTATTGGTCTGGCAGGACATTCTGGGTATCGAGTTGTACTGGATGGTGTTGGCGATGTCGGTGATCCTGCTCCTGGCGGTGGGATCCGACTACAATCTGC
    TGCTGATTTCCCGGTTGAAAAAAGAAATTGGGGCCGGATTGAACACCGGAATTATCCGTGCCATGGCTGGTACCGGGGGAGTGGTTACCGCTGCCGGCATGGTGTT
    CGCCGTTACCA
    :::::::::::::::Rv154T7.seq:::::::::::::::
    ATTGNCTTTCGGCGCCATCGGTGAGGACGGCGTGCGGGTGCTCAACGACGACGTCGTCCGCGGGACACACCTCGATGCTGCCGCCATGGACGCGGTCGAACGCAAG
    CAGCTGATCGAGCTACAACGCCGCGCGGAACGCTTCCGCCGCGGGCGTGACCGCATCCCGTTGACCGGGCGGATCGCGGTGATCGTCGATGACGGCATCGCCACCG
    GAGCGACGGCCAAGGCGGCGTGCCAGGTCGCCCGGGCGCACGGTGCGGACAAGGTGGTGCTGGCGGTCCCGATCGGCCCAGACGACATCGTGGCGAGATTCGCCGG
    GTACGCCGATGAGGTGGTGTGTTTGGCGACGCCGGCGTTGTTCTTCGCCGTCGGGCAGGGTTACCGCAACTTCACCCAGACCTCCGACGAAGAAGTGGTGGCGTTT
    TCTGGATCGTGCTC
    Clone Rv155
    :::::::::::::::Rv155SP6.seq:::::::::::::::
    ATACTCAAGCTTTTCCCGTCCGTCATCGCCCAAGCGCGTGAGGCCGAAGCGGCTGGTTACGACTCCCTGTTTGTGATGGACCACTTCTACCAACTGCCCATGTTGG
    GGACGCCCGACCAGCCGATGCTGGAGGCCTACACGGCCCTTGGTGCGCTGGCCACGGCGACCGAGCGGCTGCAACTGGGCGCGTTGGTGACCGGCAATACCTACCG
    CAGCCCGACCCTGCTGGCAAAGATCATCACCACGCTCGACGTGGTTAGCGCCGGTCGAGCGATCCTCGGCATTGGAGCCGGTTGGTTTGAGCTGGAACACCGCCAG
    CTCGGCTTCGAGTTCGGCACTTTCAGTGACCGGTTCAACCGGCTCGAANAGGCGCTACAGATCCTCGAGCCAATGGTCAAGGGTGAGCGCCAACGTTTTTCGGCGA
    TTGGTACCCACCGA
    :::::::::::::::Rv155T7.seq:::::::::::::::
    CGGCCACCGGGGCCACTCCGCACAATCTGTACCCGACCAAGATCTACACCATCGAATACGACGGCGTCGCCGACTTTCCGCGGTACCCGCTCAACTTTGTGTCGAC
    CCTCAACGCCATTGCCGGCACCTACTACGTGCACTCCAACTACTTCATCCTGACGCCGGAACAAATTGACGCAGCGGTTCCGCTGACCAATACGGTCGGTCCCACG
    ATGACCCAGTACTACATCATTCGCACGGAGAACCTGCCGCTGCTAGAGCCACTGCGATCGGTGCCGATCGTGGGGAACCCACTGGCGAACCTGGTTCAACCAAACT
    TGAAGGTGATTGTTAACCTGGGCTACGGCGACCCGGCCTATGGTTATTCGACCTCGCCGCCCAATGTTGCGACTCCGTTCGGGTTGTTCCCAGAGGTCAGCCCGGT
    CGTCATCGCCGACGCTCTCGTCGCCGGGACCAGCAGGGAATCGGCGATTTCGCCTACA
    Rv156
    :::::::::::::::Rv156SP6.seq:::::::::::::::
    ATACTCAAGCTTGGGGTGGCGCTGTCGGTCGGTGTGCTTGGCGGCGTCGGTATCAACACCGCCCACGAAATGGGGCACAAGAAGGATTCGCTGGAGCGGTGGCTGT
    CCAAAATCACCCTCGCCCAGACCTGCTACGGGCACTTCTACATCGAGCACAACCGTGGCCATCACNTCCGGGTGTCCACACCGGAGGACCCGGGGTCGGCGCGGTT
    CGGCGAAACGTTGTGGGAGTTCCTGCCCCGCAGTGTTATCGGCGGCTTGCGCTCGGCCGTTCATTTGGAGGCCCAACGGCTGCGTCGGCTCGGCGTCAGCCCCTGG
    AATCCCATGACGTATCTGCGCAACGACGTGCNCAACNCGTGGCTGATGTCNGTGGTGTTGTGGGGTGGGC
    :::::::::::::::Rv156T7.seq:::::::::::::::
    TCGCCACCGCACCGCGGCGAACGCTCAAAGGCACCTACTGGCACCAAGGCCCCACACGTCACCCTGTGACCTCCTGCGCCGACCCCGCCCGAGGTCCTGGCCGTTA
    CCACCGAACGGGCGAGCCGGGAGTCTGGTACGCATCGAACAAAGAGCAAGGTGCATGGGCGGAGTTGTTCCGCCACTTCGTCGATGACGGGGTCGATCCATTCGAG
    GTCCGTCGCCGCGTCGGTCGAGTGGCGGTCACACTCCAGGTACTCGACCTCACAGACGAGAGGACTCGATCCCATCTAGGTGTGGACGAAACAGATCTTCTGTCCG
    ACGACTACACCACCACCCAGGCCATCGCCGCCGCCCGCGATGCCAACTTCGACGCCGTACTGGCCCCGGCGGCGGCGCTCCCCGGTTGTCAAACACTTTGCCGTGT
    TCGTTCACGCACTGCCCAACATCGAGCCCGA
    Clone Rv157
    :::::::::::::::Rv157SP6.seq:::::::::::::::
    ATGAAATAAGAAGAGCACATCCCTCAGTCGGTTATCATCACTAGCGCTCGCCGCACCCGTGTAACCGATCATAGCGAGCGAACTGGCGAGGAAGCAAAGAATATCT
    GTTCTGTCAGATAGCTCTTACGCTCAGCGCAAGAAGAAATATCCCCCGCGGGAACAACTCCAGGTAGAGGTACACACGCGGATAGCCAATTCAGAGTAATAAACTG
    TGACACTCACACCCTCATCAATGATGACGAACTACACCCCGATATCCGGTCACATGACGAAGGGAAAGAGAAGGATATCATCTGTGACAAACTGCCCTCAAATTTG
    GCTTCCTTAA
    Clone Rv159
    :::::::::::::::Rv159SP6.seq:::::::::::::::
    ATACTCAAGCTTGTCGAACTCCTTCTTGAATACCGGCCGGCCATCCACAGATGCCCGGAAGAACTTCCAGGTACCCATGGCGGCTGGATCAGGGGGCGGCACAGTT
    GGTCTTGTCCTGCCTCGAGTGGCGTCGTTGTCCGGCTTGGACGGGGCTCCGACGGTACCGGAGGGCAGCGACAAAACACTTATGCACTTGGGCGACCCGCCGAGAC
    GGTGCGACACCCATCCCGACGGCACAAGCTCAGCCGCGGCCGCTCTTGTTCTTCGTCGGATCGACATTCACCCACTTCTGACCGGGCTTGGGCGAAGGAAGCAGAA
    :::::::::::::::Rv159T7.seq:::::::::::::::
    GGTATAGTCGCTGACCGGTGCAGGTTTCGACAATGTGGTGCCGGTTCGGCGGCTACGTGCCATCGAGACACTGGCGCAGGCTATCGCACCCGTTATCGGCTACGAG
    CAAATCGCGGTATGCGTTCTTGAGCATGAGTCGGCGACCTGCGTCATGGTCGACACCCACGACGGAAAGACGCAGATCGCCGTCAAGCATGTGTGCCGCGGATTAT
    CAGGACTGACCTCCTGGCTGACCGGCATGTTTGGTCGCGATGCCTGGCGCCCGGCCGGCGTGGTCGTGGTCGGCTCGGATAGCGAGGTCAGCGAATTCTCGTGGCA
    GCTCGAAAGGGTCCTGCCGGTGCCGGTCTTTGCGCAAACGATGGCGCAGGTTACGGTCGCGCGGGGTGCGGCCCTGGCGGCGGCCCA
    Clone Rv15
    :::::::::::::::Rv15SP6D2.seq:::::::::::::::
    GACACTATATNATACTCAAGCTTCAGGTCAATGTGCGCCAAGCCCTGACGCTGGCCGACCAGGCCACCGCCGCCGGANCCCTNTCTAGA
    :::::::::::::::Rv15T7.seq:::::::::::::::
    CTGTAGCCACCTGTTGCCATCCCCGTCATGCCCGACTCTGGTCATCTCGGATCCGCTGACACCCCGCTAAGGCTGCTCCTCTCGGTGCATTACCTCACCGACCGCG
    AACNCCCCCAGCTTTACGACTATCCGGATGACGGCACCTGGTTGCCGGCTAACTTCACCGTCAGCTTGGACGGCGGCGCTACCGTCGATGGCGCCAGGGGGGCGAT
    GGCCGGGCCCGGCGACCGATTCGTCNTCANCCTGTCGCGTGAACTTGCCGACGTCATCGTGGTCGGTGTGGGCACCGTGCGCATTGAGGGCTACTCCGGCGTCCGG
    ATGGGTGTCGTCAAGCGCCCGCACCGGCAGGCCCGA
    Clone Rv160
    :::::::::::::::Rv160SP6.seq:::::::::::::::
    ATACTCAAGCTTCGCACGCTCGGCGCGCGCGGTACCGCCCAGGTCGCCCAACAGATCGTCGATGTTCGCGTCGTCCGCCTCGCGCACGTGGTCTGTCACCAGTCAA
    CGTTAACGCCGCCGCACATGTCCTGCGGCCGGGCAAAAACGTGAAAAACGAGCGGGCGACTGCNATGTCATGACAGCGACGGCCGCCGATGGGCCCAGGGTCTGGC
    AAATTCGATCTGTGCGGCCAGTGCCAGCAGCGTCGCCTCGTCATACGGCCGGCCGACGAGTTGAACCGACATGGGCAGGCCGTCGCCGTCGAAGTCCCACGGCACC
    ACGGGCGCGGGCTGGCCGGTCAGATTCCAAAATTGAAAGTACGGAACCGCTGCACCACCAA
    :::::::::::::::Rv160T7.seq:::::::::::::::
    ATCGTTTCGACCAGGCGCTCCATCCGGCGAGTGGATACTCCCAGCAGGTAGCAGGTCGCCACCACGCTGGTCAGTGCGGGTTCAGCTGGCTTGCGGCGCTGCAGCA
    GCCAGTCCGGGAAATAGCTGCCCTGGCGCAGCTTGGGGATCGCGACGTCGATGGTTGCGGCACGGGTGTCGAAATCACGGTGGCGGTAGCCGTTGCGCTGATTGGA
    CCGCTCATCGCTGCGTTCGCGGTAGCCCGCCCCGCACAGGGCGTCGGCTTCAGCCCCCATCAAGGCGGCGATGAACGTCGAGAGCAGCCCGCGCAGCAGATCCGGG
    CTCGCCTGTGCGAGTTGGTCAGCCAGAAGCTGCTCGGTGTCGATAAGATGANAAGAAGTCATTGCGTTATTTCCT
    Clone Rv161
    :::::::::::::::Rv161SP6.seq:::::::::::::::
    ATACTCAAGCTTGGGTGTTGCCGATCACCGGAAGCCGCATGATCAGCCACGTTTCGCGCCGCCCGGCATACGGCGGCGTACCGATCTCCGCGTCATACACCCGCGG
    GTAATCGCCGACGGTGCCGGTTCGCGAGCCGAAGGTGACGACGCTGATTGAATCGAGTTCCAGGTCCAGCGGGTGGCGCAGCAACGGCGCGAGCTCAACNACGTCA
    ATCACGTTGTCGCTTTCTACGGTCACCGACCCGGTGACCGTAGTCGCCCGGTGCGCTCGGCCGAGAAGTTGCACCGCCACCACCGCGACAACGTCTTGCACGCGGA
    CGCCACCCCCCGGAT
    :::::::::::::::Rv161T7.seq:::::::::::::::
    GCGCMAACAGCTCGCGGCAGCCCACGACGTGCTGCGTCGGATTGCCGGCGGCGAGATCAATTCCAGGCAGCTCCCGGACAATGCGGCTCTGCTGGCCCGCAACGAA
    GGACTCGAGGTCACCCCGGTGCCCGGGGTCGTGGTGCACCTGCCGATCGCACAGGTTGGCCCACAACCGGCCGCTTGATGCCCGGTCGGCAAGCCCGGCAGTTGCC
    AAACCCAGCGTGATCAGGCTCGGCTCGCGAGTTCGGCGAAAAAGTGGCTCGCCTGATCACCTACCATCGGCCAGGATCTGCGTGTCATCACGACGCTCGCCAAGGA
    GGTTGTTGTGGTGCTATCGACGGCCTTTAGCCAGATGTTCGGAATCGACTATCCGATAGTGTCCGCGCCAATGGACTTGATCGCCGGCGGTGAGCTGGCTGCCGCN
    GT
    Clone Rv162
    :::::::::::::::Rv162SP6.seq:::::::::::::::
    ATACTCAAGCTTTCTCCGATACCCGCCATGTCGCGCACATCCAGGACTTCTGGGGGGATCCGCTGACAGCGGCGGGATCCCAAAGTGCGGATGATCGGGCCGCCTA
    CGTCGTGGTGTACCTCGTCGGTAACAACGAAACCGAAGCGTATGACTCGGTGCACGCGGTGCGGCACATGGTGGACACCACACCGCCACCGCACGGGGTGAAGGCC
    TATGTCACCGGTCCGGCAGCACTCAATGCCGACCAGGCCGAGGCCGGAAACAAAAGTATCGCTAAGGTCAGCGCGATCACGAACATGGTGATCGCAGCAATGTTGC
    TAGTGATCTATCGCTCCG
    :::::::::::::::Rv162T7.seq:::::::::::::::
    CCATGAGCACCGCCAGCCGAGCACGAGGCCAAACTCCGCCGACGCAGGCCGGTTGGACTTGTCGTGCTGGACAAGGGGTTTAGCCGCCGAAGCAGTGACGTACATC
    GGCGAAGAGCAGTTCGCCTGTCGACCGACGGCGCAAACCGTGAGGCTAGGGAAGCGAGGAGCACATGGCCGCCGACCCGCAATGTACACGCTGCAAGCAAACCATC
    GAACCCGGATGGCTATACATCACCGCCCATCGCCGCCGGTCAAGCCGGGATCGTCGATGACGGCGCAGTACTGATTCACGTGCCCGGTGAATGCCGCAGCCGGGGA
    GCACTTTCCGCCAAAACTAACCCGGTTGG
    Clone Rv163
    :::::::::::::::Rv163SP6.seq:::::::::::::::
    CGGGTGTCATTGGGCCACCGGCGGCGGCTGTCCGGGAAATGGCGGGTCCCCGGTGGTTTTGCTGAGGAGTGCTGAACCGTAGTCGAAGTGGCGGCGTCAGACTCCA
    CCCAGCCAGCAGGCAGCGCGAANCTGAATCCTCCAACCGGGTTGTCNATCCGGACAGGTTGGGGTGCGTTTGGGGCAATNACAGGTGGCGGCGGTGCGTTCGGGTC
    GGCCGGCGGAGGTGCTGCNTTGGATCCCCGGCTGGGCATTCGGCNTGTTGGCGGCGGCCGGTGGTGGGGGGGGCAACACGTGTCNGCGGTGCGGGTGGCCCT
    :::::::::::::::Rv163T7.seq:::::::::::::::
    CCAAGATCTACACCATCGAATCGACGGCGTCGCCGACTTTCCGCGGTACCCGCTCAACTTTGTGTCGACCCTCAACGCCCATTGCCGGCACCTACTACGTGCACTC
    CAACTACTTCATCCTGACGCCGGAACAANTTGACGCAGCGGTTCCGCTGACCAATACGGTCGGTCCCACGATGACCCAGTACTACATCATTCGCACGGAGAACCTG
    CCGCTGCTAGAGCCACTGCGATCGGTGCCGATCGTGGGGANACCCACTGGCGAACCTGGGTTCAACCAAACTTGAAGGTGATTGTTAACCTGGGCTACGGCGACCC
    GGCCTATGGTTATTCGACCTCGCCGCCCAAATGTTG
    Clone Rv164
    :::::::::::::::Rv165SP6.seq:::::::::::::::
    AGCTTCCCGAGTTCGGCTTTGGATCAAGACCCCAGTCCGCGGGCGCGATCCGGCBGCTCGGTGACTACATCAAGCCACAAATCGACGGCTTTCGGGGTGCCGATAC
    CGATGACGTGGCGGATGTCGAGTGTTGAGTTCTCGGCGGGGCGGATGCTCACCTGGCGATCACCTGCCTCTCGTTGACGATCGATCGTCTATGCCGCCGTCTCTGC
    GGGAACAGGCCNCCAGTACATCGCCACAGACGGGATCCACCCGCATTTCGGCTACGGTTGCTCGTTTCGGTGTTCGGACTAGTCGGTCCTGGTGACGTGCCGGTGA
    TGCGGACCGGTCCTAGCACTGACCAATGGCCAAAATGCGGGC
    :::::::::::::::Rv164T7.seq:::::::::::::::
    CGGGGGGCCTCTTAATAGTGTAGGAAAGAAGCTCTACATATTCAGGAGGATTCACCATGGCTCGTGCGGTCGGGATCGACCTCGGGACCACCAACTCCGTCGTCTC
    GGTTCTGGAAGGTGGCGACCCGGTCGTCGTCGCCAACTCCGAGGGCTCCAGGACCACCCCGTCAATTGTCGCGTTCGCCCGCAACGGTGAGGTGCTGGTCTGCCAG
    CCCGCCAAGAACCAGGCAGTGACCAACGTCGATCGCACCGTGCGCTCGGTCAAGCGACACATGGGCAGCGACTGGTCCATAGAGATTGACGGCAAGAAATACACCG
    CGCCGGAGATCAGCGCCCGCATTCTGATGAAGCTGAAGCGCGACGCCGAGGCCTACCTCGGTGAGGACATTACCGACGCGGTTATCACGACGCCCGCCTACTTCAA
    TGACGCCCAGCGTCAGGCCACCAAGGACCCGGCCAGATCGCCGGTCTCACGTGCTGCGG
    Clone Rv165
    :::::::::::::::Rv165SP6.seq:::::::::::::::
    ATACTCAAGCTTCATAACAGGCCTGTTGTGGGCGCACCCGGCTCGCCGAGTTCTGCACGCACCGCCTCAAGTGCGGCCCGCACCGCCGGCATCTCCCGGTCACGCA
    GGGCCGCGGCCCGCGCCGCAGCGACGGCGTGTTCGCGCAGTTCGCCGTCAATGATGCTGACCTGATCGGCCACCCGGGCGTTCTCGGCGTCTTCGCGTTCACTAAT
    CGCGGTGCTCAGCAGCGTCTCGACAGCCACCACCCGAGTGGCGACCAGCTGCTCCACCACGGACCGCAGCGATGCCGTCACCTCACCCGTCCAGCGGTCCACCACG
    ACACGGTCGTGCACCAGCGCGCGGGCATTCACCACCCAGGCGGTCACCGCCAGGCCGATCGCCACACCCGCCACCATCCCCGATGCAGCCAGGCCGGGAGTAAGA
    :::::::::::::::Rv165T7.seq:::::::::::::::
    CTGGTGCTGGACGGAGCCTAGTACAACTTCCTCTCCAATGCTCTTGCCCCGATCGCGGCGACCAGGATGACCCAGGACATCCTGCCGCCCGAAGTACTGGAAAAGC
    TCACACCCGAGTTCGTCGCACCGGTGGTGGCCTACCTGTGCACCGAGGAGTGTGCCGACAACCCATCGGTGTACGTCGTCAGTGGTGGTTAGGTGCAGCGAGTTGC
    GCTGTTTGGCAACGACGGCGCCAACTTCGACAAACCGCCGTCNGTACAAGATGTTGCGGCGCGGTGGGCCGAGATCNCCGATCTGTCCGGTGCGAAAATTGCTGGA
    TTCAAGTTGTAGAACTAAAT
    Clone Rv166
    :::::::::::::::Rv166SP6.seq:::::::::::::::
    ATACTCAAGCTTTTCCGGCGTCGTCCACCTGACCCAAAAAGCGCAGGTGCGCCGCCAAACGGCCCGCCTGGCCGCGCAACTGGTCGGCGTCGCCGTGGCCGACAAT
    CAGTAGCTGGACATCCGGAAACCGCTGCACCACCTTCGGCAGCGCGTCAAGCAAAAACGGCCATTCC
    :::::::::::::::Rv166T.seq:::::::::::::::
    TTTCAGATCTCATTTTTATGACATGACTGGAGATCTGTCTAGATTGCAGCTCCTGTGAGCGTGGGTACCGGATTCAAGCCGGTCGGTCACGCCGCGGTGGTACCGG
    CTTTGCGGCAGTGCTCGGCCTCGAGTTCGGCGATCGCGCGCGAAGTGCGTTTCGCGCACCAAGATCGCGGCCTAATGGCCGGCGATGACCGCGATGACCAGCGCGA
    TCCAGGAAAAACCGTTCCAACCAGTGCTGGGCGGCCATCCCCG
    Clone Rv167
    :::::::::::::::Rv167SP6.seq:::::::::::::::
    ATACTCAAGCTTCCCGACCACAAGTTGAACAGCACCGATTTCGGCGAGCACTTCGTCAACTTCCAGGGTGCCCGCACCAAGTATTTCGACAAGTATTTCCGTCGGG
    CCGCCGCCGCCGGCGCGCGGCAGGTGGTCATCCTGGCGGCGGGGCTGGACTCCCGCGCGTACCGGCTGCCTTGGCCCGACGGGACCACGGTTTTTGAGCTGGACCG
    CCCGCAGGTCCTTGATTTCAAGCGCGAGGTGCTCGCCAGCCACGGTGCCCAACCGCGCGCCCTGCGCCCGCGA
    :::::::::::::::Rv167T7.seq:::::::::::::::
    GTGTGCTGTCAATTCAGAGCTGAGCCTGATGCACTCAACTTACTGAGCATGCTAACGCTGGTCGTGCGGGTCTTGTTCCCGCGTGTCGGCAGGGCACACGCTCGGG
    GCGTAGCTGGGAGAGGCCCCGGTCAAGCCCGGAGAGCAGTGCTCAGTCCGCCAGCTTGACCGACTTTCGATGAGAACGCGCTTCTCGCCGTATTGAACTGGCGTGC
    TGACGGTCGCTGAGCAGCGCTCGCCGAGTGCGGCCGCTGATTCTTTCATCGAGCCAGGAGGCGCATTCGTGTTCGGCCGCCTGCGGGTCGGCCCCATCGTCGACGC
    GATCCGTCACCCACTCCTCGATCAGGTCTGCCTCATCGAACGGGCCAACGGTGCTGTCGGAGTATGTGTGCGTGGGCACGGCGAGCCGGGTGCTGTGGTACACCCA
    CCGTTGCATGACCAAGTTGACGCCTGACTGGCTGAGCACCGCGATCCGCTCACAGGTCGGAACGTTGGTG
    Clone Rv169
    :::::::::::::::Rv169SP6.seq:::::::::::::::
    ATACTCAAGCTTTTGGTCTAGCCGGCCGAGCCCGATACAGGTGTCATTGGCCACCGGCGGCGGCTGTCCGGGAAATGGCGGGTCCCCGGTGGTTTTGCTGAGGAGT
    GCTGAACCGTATGCGAAGTGGGCGGCGTCAGACTCCACCCAGCCAGCAGGCAGCGCGAAACTGAATCCTCCAACCGGGTTGTCGATCCGGACAGGTTGGGGTGCGT
    TTGGGGCAATGACAGGTGGCGGCGGTGCGTCCGGGTCGGCCGGCGGAAGTGCTGCGTTGGGATCGCCCGGCTGGGCATTCTGCGTGTTGGCGGCGGCCGGTGGTGG
    GGGGGCAACAGGTGTCTCCGGTGCGGGTGGCGCTGCACC
    :::::::::::::::Rv169T7.seq:::::::::::::::
    GGGGCCACTCCGCACAATCTGTACCCGACCAAGATCTACACCATCGAATACGACGGCGTCGCCGACTTTCCGCGGTACCCGCTCAACTTTGTGTCGACCCTCAACG
    CCATTGCCGGCACCTACTACGTGCACTCCAACTACTTCATCCTGACGCCGGAACAAATTGACGCAGCGGTTCCGCTGACCAATACGGTCGGTCCCACGATGACCCA
    GTACTACATCATTCGCACGGAGAACCTGCCGCTGCTAGAGCCACTGCGATCGGTGCCGATCGTGGGGAACCCACTGGCGAACCTGGTTCAACCAAACTTGAAGGTG
    ATTGTTAACCTGGGCTACGGCGACCCGGCCTATGGTTATTCGACCTCGCCGCCCAATGTTGCGACTCCGTTCGGGTTGTTCCCAGAGGTCAGCCCGGTCGTCATCG
    CCGACGCTCTCGTCGCCGGGACCCAGCACGGAAT
    Clone Rv16
    :::::::::::::::Rv16SP6.seq:::::::::::::::
    TTCTNTCTTCCCNNATTCGTNNNTCTCNTACTACCNGGGCCNCAAAACACCTTGGCNAACGCTCAAAGGCGNTACNGGCACCAAGGCCCCACACGTCACCCTGTGA
    CCTCCTGCGCCGACCCCGCCCGAGGTCCTGGCCGTTACCACTGAACGGGCGAGCCGGGAGTCTGGTACGCATCGAACAAAGAGCAAGGTGCATGGGCGGAGTTGTT
    CCGCCNCTTTTTTTATGACGGGGTCGATCCATTCGAGGTCCGTCGCCGCGTCGGTCGAGTGGCGGTCACACTCCAGGTACTCGACCTCNCAGACGAGAGGACTCGA
    TCCCATCTANGTGTGGACNAAACAGATCTTCTGTCCGACGACTACACACCACCCAGGCCATCGCCGCCGCCCGCGATGCCAACTTCNACNCCGTNCTGGCCCCGGC
    GGCGGCGCTCCCCGGTTGTCAAACACCTGCCGTGTTCGTTCACNCACTGCCCAACATCNAGCCCGANCNATCCNAGGTCCGTCCAACGCCTCCGCGGCTCNCCAAC
    CTNCTCCCNCTGATCNTCCGCACCAAACACATGCCCGACTCCNTGCNCCNATTGCTTGNATCCCT
    :::::::::::::::Rv16T7.seq:::::::::::::::
    CCGCTATCGGTCGGTGTGCTTGGCGGCGTCGGTATCAACACCGCCCACGAAATGGGGCACAAGAAGGATTCGCTGGAGCGGTGGCTGTCCAAGATCACCCTCGCCC
    AGACCTGCTACGGGCACTTCTACATCGAGCACAACCGTGGCCATCACGTCCGGGTGTCCACACCGGAGGACCCGGCGTCGGCGCGGTTCGGCGAAGAGTTGTGGGA
    GTTCCTGCCCCGCAGTGTTATCGGCGGCTTGCGCTCGGCCGTTCATTTGGAGGCCCAACGGCTGCGTCGGCTCGGCGTCAGCCCCTGGAATCCCATGACGTATCTG
    CGCAACGACGTGCTCAACGCGTGGCTGATGTCGGTGGTGTTGTGGGGTGGGCTGATCGCGGTCTTCGGCCCGGCGCTGATCCCGTTCGTCATCATCCAGGCAGTCT
    TCGGCTTCAG
    Clone Rv170
    :::::::::::::::Rv170SP6.seq:::::::::::::::
    ATACTCATGCTTGCCGAAGTTCCGATGGGTCGCGCCGGCGANCCCAGCGAAGTCGCTAGCGTGGCCGTGTTCTTGGCTTCGGATCTATCCTCGTACATGACCGGCA
    CCGTGTTGGACGTGACTGGCGGCCGGTTCATATGACACCGAGATCATTGCCACGGTACGGCAATTCGTCAAGAAGGAAATCTTTCCCAATGCACCGGCCCTCGAAC
    GTGGCAACAGCTACCCGCAAGAAATCGTCGATCGGCTGGGTGTTATTGGCTTGCTCGGTCGCCGGCTGCAAGGGTATCGACACCACCGAGTTCATTCTCCGGGCGT
    GCC
    :::::::::::::::Rv170T7.seq:::::::::::::::
    GGCGTCAACGGTGTCGGCACCGGCGTCCTGCAGTTGGTAGGCCTGCAGTTTGTGCATCAGGCCGATGCCGCGGCCCTCGTGGCCACGCATGTACAGCACCACGCCG
    CGCCCCTCACGGGCGACCATCGCCAGCGCGGCGTCCAGCTGAGGCCCGCAATCGCAGCGGCGTGACCCAAACACATCGCCGGTCAAGCACTCCGAATGCACCCGGA
    CCAGCACGTCGTCACCGTCGGCGTTGGGCCCGGCGATCTCGCCGCGGACCAGCGCGACATGTTGCACGTCCTCGTAGATGCTGGTGTAGCCGATGGCGCGAATCTC
    CCATGACGAGTCGGAATCCGCGCCTCGGCG
    Clone Rv171
    :::::::::::::::Rv171SP6.seq:::::::::::::::
    ATACTCAAGCTTCGGCCTCGCTGCAGGAGTGGGAGCCGCAGGGCTGGAAATCCGAAAAACGAGCCCGGTGATCGCACTGTCGCCGATCGGGGCCGCACCTGGTGGT
    GTTACCGATGAATCCGCACCCAAAATGTGGCTGCGGTGGCGTTTCTTGACTCCTTGGCGTCGACTCTTGTGGCAGCCACCGAGCGGTTGGTCCAGGATCTGGATGG
    GCAAAGTTGTGCGGCCCGGCCGGTGACGGCCGATGAGCTGACCGAGGTCGACAGCGCCCGTGTTGGCTGACTTGGAACCGACATGGATCGCCCCGGTTGGCGTCAC
    CTCAAGCATTTCAATGGTTAT
    :::::::::::::::Rv171T7.seq:::::::::::::::
    ATGCGTCACCCCGATGCGCCCAGATCGGGGCTTCGCAAATAAAGCACGAACAGGCGGGCAAAACGTCTATCTCGGAGCCGGAAGGGCAATCAGCCGACCGTCGACG
    AACGACACCGGCGATAACCACTTAGGCGTTGAACGGCCGGCCCAAACATTACGCCTCCGTTGATAGGCTTTCGGTCTCTTCCCCGGTCATCCCAAGCACCTTTGCG
    GCAAATTTGAACGCTTTCCTGTCCGGGCACCGGCCCCGGGCTTTGGGGTCCNTCCGA
    Clone Rv172
    :::::::::::::::Rv172SP6.seq:::::::::::::::
    ATACTCAAGCTTCAATCGCGCCGCCACAATCCAAATATGCGTCTAGCGTCTCGATGAGCGTCGGTCCGGCATCGGCTAGGGGCCGCATCACGTCGGTATGCAGGGC
    CACGATCGCCCAAGGCGTCGCCCATCAAGGGCGCGTTCGGGCAAAAATTCCCCTATCCAGCACGGGCCGCGGCGCTCCGCNCCAGCCGGCGACGGCGTTCATCCCG
    GAGATCGCCTCGCTAGCGCTGCGGTGCGCCGCGGTCAGCATGGGCGCCGTGGGGCCGATGACCACCGGGGCGT
    :::::::::::::::Rv172T7.seq:::::::::::::::
    TTCGGCGGGTCTGTAGATTGCGGTCGGCCACCCCACAGGCACTCATGAACCGCAGCCCACGATCGATCTCGGTGG
    Clone Rv173
    :::::::::::::::Rv173SP6.seq:::::::::::::::
    GCGCACCATCGCCAGTAGGTGCCCGTGGTCGGGCGCGTCGAGCCACCCGAGCGGAAACGCGAGTCCGAACAGCAACAGCAGGACGGGCGCAACCAGGGCGGTGACC
    ATGCCCCCGGCGCTGAACATCAACCACAGGAAGGGCTCCGCCGAGCGTCCGCGCGACC
    :::::::::::::::Rv173T7.seq:::::::::::::::
    CATCGTCGAACTTCGGTCCGGGTTGNTAGNACCGCAGCACCAAACGCACCCACCGACCCCCACGCTTCACGCCAACCCTTTAGTTCATTGGCGTGAACAGCAGCGT
    AGCCGGTTGCCCCGATATATGTGGAAAAATCGTTCGGACGTACAAAAAAAGTTCCTGACGCTGGCGTCAACTCGAAACTGCCTCGGAAGTCAATGATGATCCATCA
    GTCAATATTAAAGTCG
    Clone Rv174
    :::::::::::::::Rv174SP6.seq:::::::::::::::
    ATACTCAAGCTTGTCTGCTGCCTCAGCGTATGCATGCAACAGCGCATCGCGATCAACGATCAGGCGCGCCGATTTCGGGCCGCGGGCAGTGGCACTGGCCAGATGG
    CCGTTTTTTTCGAGAAACTTCAACGCCTGAGCGCTGCTTCCCATCGAGAGACCGGTGGCCTCTACAACCGATGCGACAGTTGGACCGGCGATGTTCGCCAGCAGCG
    CTTCACATACGGCAAGTNTGGCGCGG
    :::::::::::::::Rv174T7.seq:::::::::::::::
    TTGTCCAGGCGGGGAATCGGGCAGGGAGACGACACCTTCGTTCGGTTCGATCGTCGCGAACGGGTAGTTGGCCGCGACCACGTTGTTTCGGGTCAGCGCGTTGAAA
    AGTGTCGACTTGCCGACGTTGGGCAGGCCCACGATCCCCAGGCTCAAGCTCACAGA
    Clone Rv175
    :::::::::::::::Rv175SP6.seq:::::::::::::::
    ATACTCATGCTTGGCGCCTGGGTGGCAGCCCACCTGCCCACCACACGGACCGCGGTGCGGACGCGGCTGACGCGCCTGGTGGTCAGCATCGTGGCCGGTCTGCTGT
    TGTATGCCAACTTCCCGCCGCGCAACTGCTGGTGGGCGGCGGTGGTTGCGCTCGCATTGCTGGCCTGGGTGCTGACCCNCCGCNCNACAACACCGGTGGGTGGGCT
    GGGCTACGGCCTGCTATTCGGCCTGGTGTTCTACGTCTCGTTGTTGCCGTGGATCGGCGAGCTGGTGGGCCCCGGGCCCTGGTTGGCACTGGCGACGACGTNCGCG
    CTGTTCCCCGGCATCTTCGGTCTGTTCGCCGTCGTGGTACCCTGTTGCCGGGTTGGCCC
    :::::::::::::::Rv175T7.seq:::::::::::::::
    CGCCAATTCACGATATCGTTAACCGATATCCCGAGCCGATAGCTGGCGGGCTCGGGTGGTGGCCAGCGGCGCTGCGACGAAAGGTGTGACCGTCATGAAACAGACA
    CCACCGGCGGCCGTCGGCCGTCGTCACCTGCTCGAGATCTCAGCATCCGCAGCCGGTGTGATCGCGCTTTCGGCGTGTAGTGGGTCGCCGCCCGACCCCGGCAAAG
    GCCGGCCCGACACAACCCCGGAACAGGAAGTCCCGGTCACCGCGCCCGAAGNACTTGATGCGCGAACNCGGAGTGCTCCAAACGCATCCTGCTGAT
    Clone Rv176
    :::::::::::::::Rv176SP6.seq:::::::::::::::
    ATACTCAAGCTTGGGCACTGACTTCGGTACCCCCTCCGCCTTTGGCCAGCAGCAGCCACAGCGCGGTTCGCGGACCGAACGTGGACATCAATAGCCCGGAATCGGT
    GTGTGCAAGTTGGTAAACGGTGTTGATCCCAAGCTTTGCCAGCCTTTTCGTAGTCTTGGGCCCCACACCCCACAGTGCTTCGACGGTACGGTCACCCATGATGGCC
    ATCCAGTTGGCATCGGTGAGCTGATAAATGCCAGCTGGTTTCGCCAACCCGGTAGCGATCTTGGCGCGCTGCTTGTTGTCACTGATACCTATCGAGCAAGACAGCC
    CGGTTTGCGACAAAATGACTTTTCGGATCTCTTCGGCGACTTCGATGGGGTCGTCGGGA
    :::::::::::::::Rv176T7.seq:::::::::::::::
    AAAGTCCTGTGCCGGTTCGCTAAACACCCGGCGGACACTCAGACGGTGCTGGTGGTGCGGCATGGCACCGCGGGCAGCAAAGCGCACTTCTCCGGGGGACGACAGC
    AAGCGACCGCTAGACAAGAGGGGTCGTGCGCAGGCAGAAACGTTGGTACACAGCTGCTGGCGTTCGGCGCCACCGATGTTTATGCCGCCGACCGGGTGCGCTGCCA
    CCAGACGATGGAGCCACTCGCCGCGGAACTGAACGTGACCATACACA
    Clone Rv177
    :::::::::::::::Rv177SP6.seq:::::::::::::::
    ATACTCAAGCTTGGGTTCCACGCCCGCGCAGCCACGCCGTCACCTTTCCACGAGACCTCACCTGCCGATCCGAAATGGAATCGGCCGTGACGGAATTGGCGCACCG
    AACACCCAACGAGGTGGTGGCTTCGTCGCGAACCGTCACCCGAGTCGCGGCCACCGTGCGCACGGCGACGTTCTACACCCGCACCAAGATCCGAAAGCTGCAAGCT
    CCCAGCACCGATCCCGACGTCATCACCGCTGCCGCCCGGCACGTCCTTGACCTATTCGAGCTGGATCGGCCCGTCCGGTTGCTGGGAGTGCGGTTAGAACTGGCCT
    AGAACCGGCGGGCACACCGCNCCTGGGCGGGGCGAATTCTTGACCGCNCCGGCC
    :::::::::::::::Rv177T7.seq:::::::::::::::
    CGCGGTTGGCGTAGTTGGACGGGTCGCCCTCCGAGGCCAATGATGACGATGACCACGCCGATCACGATGGCCACCGAGAGGGACAACAACAGAAAGCTGACGAATC
    CCTCCTTGGCGGCCGGGGCTTTGTGGTCGCCGGTCGCGATGGGCGCGAATTTACGGCCCGCTCCCCCAGGCCGCCGCGAAGCAGGGTCCCCAGCCAGTTGGCGTAG
    GCGGAATTAACGATCAGCGCCACCGCGATAACCTGCCATGCCTCGGGCATATCGATGTGCGGCCAGAACAGGCCGAAC
    Clone Rv178
    :::::::::::::::Rv178SP6.seq:::::::::::::::
    CCAACAAGAGCATCGGGACATACGGAGTCAACTACCCGGCCAACGGTGATTTCTTGGCCGCCGCTGACGGCGCGAACGACGCCAGCGACCACATTCAGCAGATGGC
    CAGCGCGTGCCGGGCCACGAGGTTGGTGCTCGGCGGCTACTCCCAGGGTGCGGCCGTGATCGACATCGTCACCGCCGCACCACTGCCCGGCCTCGGGTTCACGCAG
    CCGTTGCCGCCCGCAGCGGACGATCACATCGCCGCGATCGCCCTGTTCGGGAATCCCTCGGGCCGCGCTGGCGGGCTGATGAGCGCCCTGACCCCTCAATTCGGGT
    CCAAGACCATCANCCTCTGCAACAACGGCGACCCGATTTCTTCNGACGGCAACCGGTGGCGAGCGCACCTAGGCTACGTGCCCGGGATGACCAACCAGGCGGCGCG
    TTTCGTCGCGAGCAGGATCTAACCGCGAGCCGCCCATAGATTCCCG
    :::::::::::::::Rv178T7.seq:::::::::::::::
    TAANACCCGTGTAATTTGGGATGGGCAAAAAGGCCAAGCACCGCGTGGCCACGAACGCCGGGAGGGACAATCTCGGGCGGCTAGGGCTTCTCGCGGGAAGGCCCGA
    ACGTACGGCGTTTCAACACGTCGCGTCNCCCTCCGACCGCGAACATTCGGGGATGGCAGCAACCTGGTAGCNCCCTGGCCGGGCGATGATCTGCAGCGTCGCCGCG
    GGTAGTCGCCGCCCGGGCGGCTACAGTCTGAAACGCGATGACCATCGATCTCTGGATGCAGCATCCGACGCAACGGTTCCTACACGGCGATATGTTCGCCTCGCTG
    CGCCGGTGGACCGGTGGGTCTATCCCGGAGACCGACNTCCCGATCGAAGCGACCGTCTCCTCGATGGACGCCGGCGGCGTCACCCTGGGTTTGCTCACCGCCTGGC
    GTGGCCCCAA
    Clone Rv179
    :::::::::::::::Rv179SP6.seq:::::::::::::::
    GTCCGCAAAAGACTCAGCGGCCGACTTTGCTCGCAGCTGGCGGTACCGCGCCACCGATTCGATGCCGTGGTCGCGGAAGAATGCCTCCCGAAATCGCACGGCCGAC
    TCCAGTTCGGCGAGCATCCGCGATGCCAGCTGCGGCTGCGCCCTGCCGGCCACGGCACCCACATGCGGCAGTTCGTCCACCTGGGCCAGCGCCCCGCCGCCGAAGT
    CCAAACAATAGAACTGCACCCGGCCCGCATCGTGGGTAGCAGCCAACGCCATGATCAGCGTCCGCAGCGCGGTTGACTTGCCCGTTTGCGGTGCACCTACGACCGC
    GACATTGCCTGCGGCCCCGGACAAGTCGATCGTCAGCGGCACCCN
    :::::::::::::::Rv179T7.seq:::::::::::::::
    CGTGGCCACGAACGCCGGAAGGGACANTCTCGGGCGGCTAGGGCTTCTCGCGGGAAGGCCCGAACGTACGGCGTTTCAACACGTCGCGTCGCCCTCCGACCGCGAA
    CATTCGGGGATGGCAGCAACCTGGCAGCTACCTGGCCGGGCGATGATCTGCAGCGTCGCCGCGGGTAGTCGCCGCCCGGGCGGCTACAGTCTGAAACGCGATGACC
    ATCGATGTGTGGATGCATCATCCGACGCAACGGTTCCTACACGGCGATATGTTCNCCTCGCTGCGCCGGTGGACCGGTGGGTCTATCCC
    Clone Rv17
    :::::::::::::::Rv17SP6.seq:::::::::::::::
    ATACTCAAGCTTTGCGGCGGGCGCCGAAATGTGAACGCACCAAACCCGCCCGCTGCGGGTCGGCGGGCCACTCGACCTCGAATTTCGCCGCCGTGACCATCCAGCC
    CGACGGCAGTTGGGCACCCGGCCCCCCGGTCGCGGCATAACTGTTGGCGTCGCCGTCATAAAGCTCGAACAGCACCGAAACCGACTCCACCACCGGCCGGTGCGCC
    TCAAAATCCACGCCGATCTCCACATACCGGGAAAACGTCGGTGTCCCATCGGGTTTCGGCTTGCCCGCCAGCTGCACACCACCGGTGGCCTCGGCCACCTTCGCGG
    CCTGAGCGCAGCTACNCATCCTGACGATCATCACCCCGCCCCCGGCTCACGCTTGGCCTCCGTGACCGCACGCATCGCCCGGTTGCGCGCACCGCGACGCCCGTAC
    AGCCGCGCGCAC
    :::::::::::::::Rv17T7.seq:::::::::::::::
    AGCTTGCCGGGACTGCGGAACAGAAGCGGCGGTTCCTACCGCGGTGTGCGGCCGGCGCGATATCGGCCTTTTTACTAACCGAACCCGATGTGGGCTCCGATCCGGC
    GCGCATGGCATCGACGGCGACGCCGATCGATGACGGCCAGGCTTACGAGCTTGAGGGTGTGAAGTTGTGGACCACCAACGGTGTGGTAGCGGACCTGCTAGTGGTT
    ATGGCGCGGGTACCGCGCAGTGAAGGGCACCGAGGGGGAATCAGCGCCTTTGTCGTCGAGGCTGATTCGCCCGGGATCACCGTGGAGCGGCGCAACAAGTTCATGG
    GACTGCGTGGCATCNAAAACGGCGTGACCCGGCTTCATCGCGTCNGGGTGCCCAAAGACAACTTGATCGGCA
    Clone Rv180
    :::::::::::::::Rv180SP6.seq:::::::::::::::
    CTCAAGCTTGGCGATGCGGGCTGGCCAAAACTGGCCGGGCGGGGGTTGGCTTGTTCAATCAAGGGTGGGTTGCCG
    :::::::::::::::Rv180T7.seq:::::::::::::::
    CCGAAGGCCCGTTCCCGGGCGTTCAGCAAGCGATCGTCGGTTGGCCCACTGCGGGTCGAATCTTGCGGCCGCGCCGGTCGTGGAACGCCCAGGTCACCCGGCGGCG
    TACC
    Clone Rv181
    :::::::::::::::Rv181SP6.seq:::::::::::::::
    ATACTCAAGCTTTTTTCTGCTCATGAAGGTTAGATGCCTGCTGCTTAAGTAATTCCTCTTTATCTGTAAAGGCTTTTTGAAGTGCATCACCTGACCGGGCAAATAG
    TTCACCGGGGTGAGAAAAAAGAGCAACAACTGATTTAGGCAATTTGGCGGTGTTGATACAGCGGGTAATAATCTTACGTGAAATATTTTCCGCATCAGCCAGCGCA
    GAAATATTTCCAGCAAATTCATTCTGCAATCGGCTTGCATAACGCTGACCACGTTCATAAGCACTTGTTGGGCGATAATCGTTACCCAATCTGGATAATGCAGCCA
    TCTGCTCATCATCCAGCTCGCCAACCAGAACACGATAATCACTTTCGGTAAGTGCAGCAGCTTTACGACGGCGACTCCCATCGGCAATTTCTATGACACCAGATAC
    TCTTCGACCGAACGCCGGTGTCGTTGACCA
    Clone Rv182
    :::::::::::::::Rv182SP6.seq:::::::::::::::
    CTCAAGCTTGGTGCCGACATGGCCGGGCTGGAGCCCGCGTATGGCAAGGTTCCGCTCAATGTGGTTGTGATGCAGCAGGACTACGTTCGCCTCAATCAGCTCAAAC
    GTCACCCCCGTGGCGTGCTGCGCAGCATGAAGGTCGGCGCCCGCACGATGTGGGCGAAGGCAACAGGTAAAAACCTGGTCGGCATGGGTCGAGCCCTCATTGGGCC
    GTTGCGGATCGGGTTGCACCGCGCCGGAGTGCCGGTCGAACTCAACACCGCCTTCACCGATCTTTTCGTCAAAAATGGCGTCGTGTCCGGGGTATAC
    :::::::::::::::Rv182T7.seq:::::::::::::::
    CCGAAGCGTGGGAAATCCTGACCGAATACCGCGACGTGCTGGACACTTTGGCCGGCGAGCTGCTGGAAAAGGAGACCCTGCACCGACCCGAGCTGGAAAGCATCTT
    CGCTGACGTCTAAAAGCGGCCGCGGCTCACCATGTTCGACGACTTCGGTGGCCGGATCCCGTCGGACAAACCGCCCATCAAGACACCCGGGGGAGATCGCGATCGA
    AACGCGGCGAAACTTGGGCC
    :::::::::::::::Rv183SP6.seq:::::::::::::::
    CGACTCGACAAGCATTCTTGACAGTTGTTTTGGCTCGGCATGGTTAGCCAAGGTTCTGCGGTCCCACCAGATCATCTTGGTCCGGTAGCGCTCGTCCGGGTATGCT
    GCCGCCGGGATTCTCGCTGCTATTACTCCCCCCGAAAAACGCCACCGGTCCAGCGCGTGGGCCGCCGCGGTCCCCATCACAAACTGAACCCCCAAGACCCCACATG
    CTTAGCGGTAGGGCGCGCGCCAAGGCGGCAGCAATCGCATCACTGCGCTGCGCGTCACTATTAACCCACCCGGACTTCACTTCCACGACCCCGAATGGCGCCCGGT
    CATTGATCATCTTGCGCACCGCGGATAATCCGGGATTGCCAGCCCATTCGACTACCGCATGCGAGTCATCGGCTGACCGCAGCGGTCCGATTACCCGAGCGCCCCG
    ANTACATCTCCTCCAATATCAATGGGCGCAA
    Clone Rv183
    :::::::::::::::Rv183T7.seq:::::::::::::::
    GCGGTNTAGCTTCCCGTCGTACCGGCGACCGCCAGCCGAGAAGCTCGTTTTCCCAGTGTTGCTGGGGATTCTCACGCTGCTGCTGAGTGCGTGCCAGACCGCTTCC
    GCTTCGGGTTACAACGAGCCGCGGGGCTACGATCGTGCGACGCTGAAGTTGGTGTTCTCCATGGACTTGGGGATGTGCCTGAACCGGTTCACCTACGACTCCAAGC
    TGGCGCCGTCTCGTCCGCAGGTCGTTGCTTGCGATAGCCGGGAGCCCCGGATCCGCAATGACGGATTCCATGCCAACGCTCCGAGTTGCATGCGGATCGACTACGA
    ATTGATCACCCAGAACCATCGGGCGTATTACTGCCTGAAGTACCTGGTGCGGGTCGGATACTGCTATCCGGCGGTGACGACCCCCGGCAAGCCGCCATCCGTGCTG
    CTGT
    Clone Rv184
    :::::::::::::::Rv184SP6.seq:::::::::::::::
    CTCAAGCTTGGGCGTGACGGCCACCGGGGCCACTCCGCACAATCTGTACCCGACCAAGATCTACACCATCGAATACGACGGCGTCGCCGACTTTCCGCGGTACCCG
    CTCAACTTTGTGTCGACCCTCAACGCCATTGCCGGCACCTACTACGTGCACTCCAACTACTTCATCCTGACGCCGGAACAAATTGACGCAGCGGTTCCGCTGACCA
    ATACGGTCGGTCCCACGATGACCCAGTACTACATCATTCGCACGGAGAACCTGCCGCTGCTAAAGCCACTGCGATCGGTGCCGATCGTGGGGAACCCACTGGCGAA
    CCTGGTTCAACCAAACTTGAAGGTGATTGTTAACCTGGGCTACGGCGACCCGGCCTATGGTTATTCC
    :::::::::::::::Rv184T7.seq:::::::::::::::
    CGGGTGTCATTGGCCACCGGCGGCGGCTGTCCGGGAAATGGCGGGTCCCCGGTGGTTTTGCTGAGGAGTGCTGAACCGTAGTCGAAGTGGGCGGCGTCAGACTCCA
    CCCAGCCAGCAGGCAGCGCGAAGCTGAATCCTCCAACCGGGTTGTCGATCCGGACAGGTTGGGGTGCGTTTGGGGCAATGACAGGTGGCGGCGGTGCGTTCGGGTC
    GGCCGGCGGAGGTGCTGCGTTGGGATCGCCCGGCTGGGCATTCGGCGTGTTGGCGGCGGCCGGTGGTGGGGGGGCAACANGTGTCGCCGGTGCGGGTGGCGCTGCA
    Clone Rv185
    :::::::::::::::Rv185SP6.seq:::::::::::::::
    NCTTGATATTGGCGTCAACGGTGTCGGCACCCGGCGTCCTGCAGTTGGTAGGCCTGCAGTTTGTCGATCAGGCCGATGCCGGGCCCTCGTGGCCACGCATGTACAG
    CACCACGCCGCGCCCCTCACGGGCGACCATCGCCAGCGCGGCGTCCAGCTGAGGCCCGCAATCGCAGCGGGGTGACCCAAACACATCGCCGGTCAAGCACTCCGAA
    TGCACCCGGACCAGCACGTCGTCACCGTCGGCGTTGGGCCCGGCGATCTCGCCGCGGACCAGCGCGACATGTTCCACGTCCTCGTAGATGCTGGTGTAGCCGATGG
    CGCGAAACTCCCCATGACGAGTCGGAATCCGCGCCTCGGCGACCCGCTCAATGTGCTTCTCGTGCTTGCGCCGCCATTCGATCAAGTCAGCAATGGTGATCAGCGC
    CAGACCGTGCTCNTCGGCG
    :::::::::::::::Rv185T7.seq:::::::::::::::
    CATAAGGGCCGGCGTACCCGGTACCGGCCGCGGGCCTACCACGTGCCGGAACTGGAAGCGCAGTAAGCCCTCAACGCGCCACCGCTTTGGCCCGCGCGCCCGGCGT
    AGGCGCATCGGCGGTGGCCGTGGGGCGGCGCACTGCGACCTCACCAGCGGCTTTCGAGCTTTGTTCGATCAACCGGCCAGCATGGTCGAGGATGCATTCGAGACCA
    TATTCGAAATTGGTTTCATCGGGGGCCCCGATCCGATGCCCCCTCCCAGTTGCGTGAGCAAGCAGCGGAGTCGTCGCGGGATCGATGGCCACGGGGTGTTCAATGG
    CGGATGGTCCGCTGCCCGCCGACTGGCTCTTGCGGGAGAGCCGATCTAGCACCACCGATCCGCGCACGTGGACCGAAACCGCCGAGTAGATGTCGAAAGCGT
    Clone Rv186
    :::::::::::::::Rv186SP6.seq:::::::::::::::
    CGTCCTTTTCCCCAAGATAGAAAGGCAGGAGAGTGTCTTCTGCATGAATATGAAGATCTGGTACCCATCCGTGATACATTGAGGCTGTTCCCTGGGGGTCGTTACC
    TTCCACNAGCAAAACACGTAGCCCCTTCAGAGCCNNATCCTGAGCAANATGAACAGAAACTGAGGTTTTGTAAACGCCACCTTTATGGGCAGCAACCCCGATCACC
    GGTGGAAATACGTCTTCAGCACGTCGCAATCGCGTACCAAACACATCACGCATATGATTAATTTGTTCAATTGTATAACCAACACGTTGCTCAACCCGTCCTCGAA
    TTTCCATATCCGGGTGCG
    Clone Rv187
    :::::::::::::::Rv187SP6.seq:::::::::::::::
    CTCAAGCTTCATGTCCGTACGGCTCGGGTACGCTTCCGTCGCAGTGTGCGAGTGATAAATGACGACCGGGACCTCGTCGGCATCTTCCATAGCCCGCCACACCTTC
    AGTTGCTCACCGGAATCCAACCGGTAGAAGGTCGGCGAGCGCTCGGCATTGGTCATCGGGATATGCCGCTCGGGACGGTCAGAGCCCTCGGGTCCGGCCAGCACTC
    CGCAGGCTTCGTCGGGGTGGTCGCGACACGCATGGGCCACCATCGCATTCAC
    :::::::::::::::Rv187T7.seq:::::::::::::::
    NCGCCGCCAGCCACCACGCGCGGGTCGGGCGCCGGGCCCGGGCCGCCAGGCTGCTCCGCTCGGTGATGGCACGCCACCGCGACACCACCCGGCTGCGCTACGTCGA
    GCCATACCGGGCGGAGCTACATCGGCTCGGCCGCCCAGTGTTCGGGCCCTCTTTCGAGGTCGAGGTCGATACCGATTTGCGCATCCGCAGCCGCACCCTGGACGAC
    AGAACCGTGCCCTACGANTGCTTGTCGGGCGGGGCCAAAGAACAGCTTGGCATCCTGGCGCGATTGGCCGGCGCGGCGCTGGTCTCCAAAGAAGACGCCCTTCCGG
    TGCTGAT
    Clone Rv188
    :::::::::::::::Rv188SP6.seq:::::::::::::::
    CGCCACGTTCATGGGCAACAACCCCGATCACCGGTGGAAATACGTCTTCAGCACGTCGCAATCGCGTACCAAACACATCACGCATATGATTAATTCGTCCAATTGT
    ATAACCAACACGTTGCTCAACCCGTCCTCGAATTTCCATATCCGGGTGCGGTAGTCGCCCTGCTTTCTCGGCATCTCTGATAGCCTGAGAAGAAACCCCAACTAAA
    TCCGCTGCTTCNCCTATTCTCCAGCGCCGGG
    Clone Rv189
    :::::::::::::::Rv189SP6.seq:::::::::::::::
    ATACTCAAGCTTCAACCGATTGACGCATTGTGCGAACTGACGGCGCCCGCGCATGGCCAATCCGGAAGACCATCATTGGCCAGTGGGGGGGCGCTAACAGGTTCCA
    GCCCCCCACCAGTGCCGCTCGAACATGCGGTGCAACCCATTCGCAGGCCGGCAGGGAAAGCACCGCGGAAGCCGCAAAGGGCTGCAGTTCCGCGCCCAATAGTGTC
    GTCCGCAACCAGATGCGCTCGAAAACCGCGCCGGCAGTCAGCGCACCCGACGCGAGGTCGAGAGACGTCGTCAGCGCGCCCACATGGGGTGCCAATCGGCACGGCA
    GGTAGGCCGCGCGCAACCCGAACGCGTGGTGCATGCCCACGGTCCGCAGGAGGCGCAGCACCCGCCAATGCCGAAGCCCACGAAACATCGGGCGCATCCACGCTTC
    AACCTC
    Clone Rv18
    :::::::::::::::Rv18T7.seq:::::::::::::::
    AGCTTTTGGCAGGGTCTCCTTCGAATTCGGCGTGCACCGCTATGGGTTGCAGCAGCGGCTGGCGCCGCACACCCCACTGGCCCCGGGTGTTTTGCCCCGAACCCGG
    ATCATGGTGAGCGAAAAGGAGATTCGCCTGTTCGATGCTGGGATTCGCCACCGCGAGGCCATCGACCGATTACTCGCCACCGGGGTGCGAGAGGTGCCGCAGTCCC
    GCTCCGTCGACGTCTCCGACGATCCATCCGGCTTCCGCCGTCGGGTGGCGGTAGCCGTCGATGAAATCGCTGCCGGCCGCTACCACAAGGTGATTCTGTCCCGTTG
    TGTCGAAGTGCCTTTCGCGATCGACTTTCCGTTGACCTACCGGCTGGGGCGTCGGCACAACACCCCGGTGAGGTCGTTTTTGTTGCAGTTGGGCGGAATCCGTGCT
    CTGGGTTACAGCCCGAATCGTCAC
    Clone Rv190
    :::::::::::::::Rv190SP6.seq:::::::::::::::
    ATACTCAAGCTTTGTCACACCAACTGTTTCCACCAGGCGCTCCATCCGGCGAGTGGATACTCCCAGCAGGTAGCAGGTCGCCACCACGCTGGTCAGTGCGCGTTCA
    GCTCGCTTGCGGCGCTGCAGCAGCCAGTCCGGGAAATAGCTGCCCTGGCGCAGCTTGGGGATCGCGACTTCTATGGTTGCGGCACGGGTGTCGAAATCACGGTGGC
    GGTAGCCGTTGCGCTGATTGGACCGCTCATCGCTGCGTTCGCGGTAGCCCGCCGCGCACAGGGCGTCGGCTTCAGCCCCCATCAAGGCGGCGATGAACGTCGAGAG
    CAGCCCGCGCAGCAGATCCGGGCTCGCCTGTGCGAGTTGGTCAGCCAGAACCTGCTCGGTGT
    :::::::::::::::Rv190T7.seq:::::::::::::::
    CCTTAAGCCCCGCAGGGCCCGGCACGCGCGGTACCGCCCAGGTCGCCCCAACAGTCGTCGATGTTCGCGTCGTCCGCCTCGCGCACGTGGTCTGTCACCAGTCAAC
    GTTAACGCCGCCGCACATGTCCTGCGGCCGGGCAAAAACGTGAAAAACGAGCGGGCGACTGCAATGTCATGACACCGACGGCCGCCGATGGGCCCAGGGTCTGGCA
    GATTCGATCTGTGCGGCCAGTGCCAGCAGCGTCGCCTCGTCATACGGCCGGCCGACGAGTTGAACCGACATGGGCAGGCCGTCGCCGTCGAAGTCCCACGGCACCA
    CGGCCGCGGGCTGGCCGGTCAGATTCCAGACTTGAAAGTACGGAACCCGTGCACCACCAGCAGCAACGTCGAAACTGCACCCCGGCGTTGGTAGGCGCCGATGCCG
    GGACGGGCCGGTCGCGGCGCCTGGCGTCACAACTACGTCGACATCGTCGAAGATCGACTGGATCGGCTGCTCACACCACTCGGCGGCCGCAGGCCGCCATCCGCCG
    TC
    Clone Rv191
    :::::::::::::::Rv191SP6.seq:::::::::::::::
    AGCTTTTTGAGCGTCGCGCGGGGCAGCTTCGCCGGCAATTCTACTAGCGAGAAGTCTGGCCCGATACGGATCTGACCGAAGTCGCTGCGGTGCAGCCCACCCTCAT
    TGGCGATGGCGCCGACGATGGCGCCTGGACCGATCTTGTGCCGCTTGCCGACGGCGACGCGGTGGGTGGTCAAGTCCGGTCTACGCTTGGGCCTTTGCGGACGGTC
    CCGACGCTGGTCGCGGTTGCGCCGCGAAAGCGGCGGGTCGGGTGCCATCAGGAATGCCTCACCGCCGCGGCACTGCACGGCCAGTGCCGCGGCGATGTCAGCCATC
    GGGACATCATGCTCGCGTTCATACTCCTCGACCAGTCGGCGGAACAGCTCGATTCCCGGACCGCCCAGCGCATTGGTGATGGAATCGGCGAACTTGGCCACCCGCT
    GGGTGTTGACATCCTCGACGGTGGGCAATTGCCCCCGGTAACGTTTGCCGCCT
    :::::::::::::::Rv191T7.seq:::::::::::::::
    CGGTCCGACCCTGTTCGACGGCTACCTGAATCAACCCGATGCCACCGCCGCGGCGTTCGACGCCGACAGCTGGTACCGCACCGGCGACGTCGCGGTGGTCGACGGC
    AGTGGGATGCACCGCATCGTGGGACGCGAGTCGGTCGACTTGATCAAGTCGGGTGGATACCGGGTCGGCGCCGGTGAAATTGAAACGGTGCTGCTCGGGCATCCGG
    ACGTGGCGGAGGCGGCAGTCGTCGGGGTGCCCGACGATGATCTAGGCCAGCGGATCGTTGCCTACGTAGTCGGCTCAGCGAATGTCGATGCGGACGGGCTTATCAA
    CTTTGTTGCCCAACAACTTTCGGTGCACAAGCGCCCGCGCGAGGTGCGTATCGTANATGCGCTGCCGCGCAACGCCTTGGGGAAAGTGCTCCAGAACATTGCTGTC
    AGAAGCTGANCTACGCGAATTATCGTGTTACGCTGGA
    Clone Rv192
    :::::::::::::::Rv192SP6.seq:::::::::::::::
    ATACTCAAGCTTGCCGAAGTTCCGATGGGTCGCGCCGGCGAGCCCAGCGAAGTCGCTACCGTGGCCGTGTTCTTGGCTTCGGATCTATCCTCGTTCATGACCGGCA
    CCGTGTTGGACGTGACTGGCGGCCGGTCCATATGACACCGAGATCATTGCCACGGTACGGCAATTCGTCAAGAAGGAAATCTTTCCCAATGCACCGGCCCTCGAAC
    GTGGCAACAGCTACCCGCAAGAAATCGTCGATCGGCTGGGTGTTATTGGCTTGCTCGGTCGCCGGCTGCAAGGGTATCGACACCACCGAGTTCATTCTCGGGCGTG
    CCGGCGCATTCGAGCTGGCGGTGCGCGCTGCCCAGCACCGTCATAGGTACTTGACGATGGTCCACGTCGGACGAGCGCCTCCACGTCGCTGCCGAACGGTATGCAT
    GGCGGCTACGATTCTC
    :::::::::::::::Rv192T7.seq:::::::::::::::
    CGGTGTCGGCACCGGCGTCCTGCAGTTGGTAGGCCTGCAGTTTGTGCATCAGGCCGATGCCGCGGCCCTCGTGGCCACGCATGTACAGCACCACGCCGGGCCCCTC
    ACGGGCGACCATCGCCAGCGCGGCGTCCAGCTGAGGCCCGCAATCGCAGCGGCGTGACCCAAACACATCGCCGGTCAAGCACTCCGAATGCACCCGGACCAGCACG
    TCGTCACCGTCGGCGTTGGGCCCGGCGATCTCGCCGCGGACCAGCGCGACATGTTCCACGTCCTCGTAGATGCTGGTGAGCCCGATGGCGCGAAACTCCCCATGAC
    GAGTCGGAATCCGCGCCTCGGCGACCCGCTCAATGTGCTTCTCGTGCTTGCGCCGCCATTCGATCAAGTCAGCAATGGTGATCAGCGCCAGACCGTGCTCATCGGC
    GAACACCGCAATTCATCGGTGTTGCGCCATCGAGCCCTCATCTTTTGGCTGACGATCTCGCAAATCGCCCCCGCGGGTTGCAGCCGGCAT
    Clone Rv193
    :::::::::::::::Rv193SP6.seq:::::::::::::::
    ATACTCAAGCTTTGGGTGAAAGCCGATCACCGGAAGCCGCATGATCAGCCACGTTTCGCGCCGCCCGGCATACGGCGGCGTACCGATCTCCGCGTCATACACCCGC
    GGGTAATCGCCGACGGTGCCGGTTCGCGAGCCGAAGGTGACGACGCTGATTGAATCGAGTTCCAGGTCCAGCGGGTGGCGCAGCAACGGCGCGAGCTCAACGACGT
    CAATCACGTTGTCGCTTTCTACGGTCACCGACCCGGTGACCGTNCTCGCCCGGTGCGCTCGGCCGATAAGTTGCACCGCCACCACCGCGACACCGTCTTGCACGCG
    GACCCACCCCCGGATCCGTTGTTGGCC
    :::::::::::::::Rv193T7.seq:::::::::::::::
    AGCTTGCTGGCATCCGCTCCAGTAGCGCCCCGCGCGTGGCTTCCAGCGCCCGCAGATGCTCCATGAGCCGGCCGGTCGAGTCGGCGCCGGCGTTCACCGCCACCCG
    CCAGGAGCTGGCGGCCAGCATCTCCGCCTTCACGCATTGCGCGATCACAGAGAGAATATACGTCTCATATTCGTTGGAGGTCGTCGCAGGCAATCGGTCGATGACG
    GATTTGATGGCATCGAGCTGTGCTTCGGCGTAGCCCTCCAGCACGTCGGTATCGCTGTGGCGGTCCACGACGACCGCACCGGCGCGGCGGACAGCCGTCGGGTTGG
    ACGMTGTGCGGCGATCAGTCCGGCCAGCTCCGCCTCGGGATCAGCGGC
    Clone Rv194
    :::::::::::::::Rv194SP6.seq:::::::::::::::
    ATACTCAAGCTTGCTGCAGCTTCCTATGACTGCTCCCGAAACCTGGGGGTGTGCCTGCTGTGTATGCACGGCATACGGACATCCTTCCCCTGAGACCCGCGGTCGA
    ACCAGCCACGTGTCCATCATCAGGGGTCAACCCGGGCCAAGGGCGACGGCACGCCAAGTTCGCCGACCGTTAACCTAGTGCTGTTAGCTTCATTTGCTGCGAGCAA
    AACAGCTGGTCGGCCGTTAGGAACTGAATTGAAACTCAACCGATTTGGTGCCGCCGTAGGTGTCCTGGCTGCGGGTGCGCTGGTGTTGTCCGCGTGTGGTAACNAC
    NACAATGTGACCGGGGGAGGTGCAACCACTGGCCAGGCGTCGGCGAAGGTCGATTGCGGGGGGAAGAAGAACTCAAAGCCAGTGGGTCGACGCGCAGGCCAACGC
    :::::::::::::::Rv194T7.seq:::::::::::::::
    AGCTTGACGCGGAGACGGACACATTGCGAACATTGATGACAAAATAGAAATCATTGATGGTTTGAGTCACCAGGCCGATCAAGCCTTCGCCGAGCCAAATTGCAAT
    CAAGAGGCCCAAGCCCGTACCAATCAGCCCGGCAACGAGGGATTCCGTCATTATCAGCCAAAATAACTGCTCTCGGGTTACACCCAAACAGCGCAATATGGCGAAA
    AACGGTCGCCGTTGCACGACATTAAATGTCACGGTATTGTAGATTAAAAAGATACCCACCAACAAGGCAATCAAACTGAGAGCGGTTAAATTGACCGTAAAAGCGT
    CCGTCATCTGTTTGACGGTGTCCCGTTGGGTATCCGACGTTTCCATACGCACACCGGCCGGCAGTCTTTGTTGGATGCGTGTTGCAGTGGCCTCATCTTTGATGAT
    CAAATCGATGTGGCTCAGTGTTCCGGGCA
    Clone Rv195
    :::::::::::::::Rv195SP6.seq:::::::::::::::
    ATACTCAAGCTTCGGCTCAGGCGGCGCTGCTGGTAAAGTCGCTGACCGGTGCAGGTTTCGACAATGTGGTGCCGGTTCGGCGGCTACGTGCCATCGAGACACTGGC
    GCAGGCTATCGCACCCGTTATCGGCTACGAGCAAATCGCGGTATGCGTTCTTGAGCATGAGTCGGCGACCGTCGTCATGGTCGACACCCACGACGGAAAGACGCAG
    ATCGCCGTCAAGCATGTGTGCCGCGGATTATCAGGACTGACCTCCTGGCTGACCGGCATGTTTGGTCGCGATGCCTGGCGCCCGGCCGGCGTGGTCGTGGTCCGCT
    CGGATAGCGAGGTCAGCGAATTCNCNTTGGCAGTCCAAAGGGTCCTGCCGGTGCCGGTCTTTGCGCAAACNAAGGCNCAGGTTA
    :::::::::::::::Rv195T7.seq:::::::::::::::
    TGATCGCGCATCACCTGCTTCATAAACTGGAAGCAGCGCAGCGCTTCCTTTTCGGCCGCAACATGAGCCAGCCTCTCGTCGGCGGTCGGGTGCAGGTGCTCGGGCA
    GCTCGGCCGCGACAGCCGCCTGACCCTGAAACCAGCTTCCATATCCCGCGACGAACGACGCCAGTCCGCTACGTAACCCCTCCGCGACTGTCCATGGACAACAGCG
    CGTTCTCCACCGACCGGGCCCGGGTGTGGGGTGTTTCGGCGACCGGCAGCCAGGTGGTCCACACTGCCGACGGGCGCCGCGAGCCGTTCACCGACCAGGCCGCCGA
    GCAAGTCCGCCCGATCGCATACTCCAACCGGTTGCGGTACTGCAGGTTCAGCTGGCGTACTCCTCGTCGCGCTCGGCGAGGTCTTGCTCCAGCACGTCGCANACGG
    CAG
    Clone Rv196
    :::::::::::::::Rv196SP6.seq:::::::::::::::
    CAAAGCGCGAACTGCTCGCGGCAGCCCACGACGTGCTGCGTCGGATTGCCGGCGGCGAAATCAATTCCAGGCAGCTCCCGGACAATGCGGCTCTGCTGGCCCGCAA
    CGAAGGACTCGAGGTCACCCCGGTGCCCGGGGTCGTGGTGCACCTGCCGATCGCACAGGTTGGCCCACAACCGGCCGCTTGATGCCCGGTCGGCAAGCCCGGCAGT
    TGCCAAACCCAGCGTGATCAGGCTCGGCTCGCGAGTTCCGGGAAGAAGTGGCTCCGCCTGATCACCTACCATCCGCCAGGATCTGCGTGTCTTCACCACGCCCGCC
    AAGGAGGTTGTTGTGGTGCTATCGACCGN
    :::::::::::::::Rv196T7.seq:::::::::::::::
    CCGGAAGCCGCATGATCAGCCAAGTTTCGCGCCGCCCGGCATACGGCGGCGTACCGATCTCCGCGTCATACACCCGCGGGTAATCGCCGACGGTGCCGGTTCGCGA
    GCCGAAGGTGACGACGCTGATTGAATCGAGTTCCAGGTCCAGCGGGTGGCGCAGCAACGGCGCGAGCTCAACGACGTCAATCACGTTGTCGCTTTCTACGGTCACC
    GACCCGGTGACCGTNGTCGCCCGGTGCGCTCGGCCGAAAANTTGCACCGCCACCACCGCGAAACCGTCTTGCACNCCGGAAGCCACCCCCGATCCGTTGTTGGGCC
    AGGTTATTGGGT
    Clone Rv19
    :::::::::::::::Rv19SP6.seq:::::::::::::::
    CCGGAACCGCCGACGGCACGGTATAACGCCTCCGCATATGGGTCGACAACCAGCGGGTCGGACTTCTGGGCTTCTAGCGTTCGCGCNGTCGCGACAAACAGCGCGG
    TCGAACCGACACTCGTTGTGATGTCCTAGCTATCACGTTCGGTACGCACCCAATCGAGTCTAGCGCGGGTAGNTCAGCCCCGATCTCCANGCTCCGCCGAGCCAGG
    CGC
    :::::::::::::::Rv19T7.seq:::::::::::::::
    CTGGTTTATGTCCCGTTGAAGTTCCATCACCCGATGTGGCGGGAGCACTGCCAGGTCGATCTCAACTACCACATCCGGCCGTGGCGGTTGCGCGGGCCGGGGGGTC
    GGCGCGAACTCGACGAGGCGGTCGGAGAAATCGCCAGCACCCCGCTGAACCGCGACCACCCGCTGTGGGAGATGTACTTCGTTGAGGGGCTTGCCAACCACCGGAT
    CGCGGTGGTTGCC
    Clone Rv1
    :::::::::::::::Rv1SP6D2.seq:::::::::::::::
    CCGAGCAGTTGGGAATCGCTCTGCANCAAACCAATATTCTGCGCGACGTCGCGCGACGAGCTGGACCGATTAGGCGTACGCCTCCGNCTGGACGACACCGGGGCAC
    TCGATGACCCCGACGCCTACGCTCGCAGGATATTGTTCGCCGGACCCCTCTCTAG
    :::::::::::::::Rv1T7.seq:::::::::::::::
    TATATAATACTCAAGCTTGCCGACGCCAACGCTCGCGCGATGTTGTTAGCCCGACCCGGCTCTTACATGGCACCGGTGCCCCACACGTCAGCCTGTGACGTCCTGC
    ACCGCGACTCTTTACATAGAATGTGGATTGCCGGATTGGGGATGTCCGGCATCGCTCAATCTGTAGTCCGCGTTGTCCCGCGAGGGCCATGTGGATGGGGGGAAGG
    ATCCGTGGCGTCCGGGATCACCATGGGG
    Clone Rv201
    :::::::::::::::Rv201SP6.seq:::::::::::::::
    ATACTCAAGCTTGCCGAAGTTCCGATGGGTCGCGCCGGCGAGCCCAACGAAATCGCTAGCGTGGCCGTGTTCTTGGCTTCGGATCTATCCTCGTACATGACCGGCA
    CCGTGTTGGACGTGACTGGCGGCCGGTTCATATGACACCGAGATCATTGCCACGGTACGGAAATTCGTCCAGAAGGAAATCTTTCCCAATGCACCGGCCCTCGAAC
    GTGGCAACAGCTACCCGCAAGAAATCGTCAATCGGCTGGGTGTTATTGGCTTGCTCGGTCGCCGGCTGCGAGGGTTTCTACACCACCGAGTTCATTCTCGGGCGTG
    CCGGCGCATTCGAACTGGCGGTGCGCGCTG
    :::::::::::::::Rv201T7.seq:::::::::::::::
    GCACCGGCGTCCTGCAGTTGGTAGGCCTGCAGTTTGTGCATCAGGCCGATGCCGCGGCCCTCGTGGCCACGCATGTACAGCACCACGCCGCGCCCCTCACGGGCGA
    CCATCGCCAGCGCGGCGTCCAGCTGAGGCCCGCAATCGCAGCGGCGTGACCCAAACACATCGCCGGTCAAGCACTCCGAATGCACCCGGACCAGCACGTCTTCACC
    GTCGGCGTTGGGCCCGGCGATCTCGCCGCGGACCAACGCGACATGTTCCACGTCCTCGTACATGCTGGTGTAGCCGATGGCGCGAAACTCCCCANGACAAGTCGGA
    ATCCGCGCCTCGGCGAACCGCTCAATGTGCCTCTCGTGCTTGCGCCGCCATTC
    Clone Rv204
    :::::::::::::::Rv204SP6.seq:::::::::::::::
    TGGTCCGTGTGCGCATACCAATACAACGCGCCGGGCACCTGACGCGGCGGCCGCAACCAATCGGTGGCCATCGCCATCTTCTGCTACCCGGTCAACGGACGCACCT
    TCTCCTGGCCGACGTAGTGCGCCCACCCGCCGCCGTTGCGTCCCATCGATCCGGTCAAC
    Clone Rv205
    :::::::::::::::Rv205SP6.seq:::::::::::::::
    GGCGTGTTGGCCACCGGGGCCACTCCGCACAATCTGTACCCGACCAAGATCTACACCATCGAATACGACGGCGTCGCCGACTTTCCGCGGTACCCGCTCAACTTTG
    TGTCGACCCTCAACGCCATTGCCGGCACCTACTACGTGCACTCCAACTACTTCATCCTGACGCCGGAACAAATTGACGCAGCGGTTCCGCTGACCAATACGGTCGG
    TCCCACGATGACCCAGTACTACATCATTCGCACGGAGAACCTGCCGCTGCTAAAGCCACTGGCGATCGGTGCCGATCGTGGGGAACCCACTGGCGAACCTGGTTCA
    ACCAAACTTGAAGGTGATTGTTTACCTGGGCTACGGCGACCCGGCCTATGGTTATTCGACCTCCCCGCCCAA
    :::::::::::::::Rv205T7.seq:::::::::::::::
    CGTCCGTGNCCCCTCAANCGCGTGNNGCCGAAGCGGCTGGTTACGACTCCCTGTTTGTGATGGACACTTCTACCAACTGCCCATGTTGGGGACGCCCGACCAGCCG
    ATGCTGGAGGCCTACACGGCCCTTGGTGCGCTGGCCACGGCGACCGANCGGCTGCAACTGGGCGCGTTGGTGACCGGCAATACCTACCGCAGCCCGACCCTGCTGG
    CAAAGATCATCACCACGCTCGACGTGGTTAGCGCCGGTCGAGCGATCCTCGGCATTGGAGCCGGTTGGTTTGAGCTGGAAACACCGCCAGCTCGGCTTCGAGTTCG
    GCACTTTCAGTGACCGGTTCAACCGGCTCGAAGAGGCGCTACAGATCCTCCAGCCAATGGTCAAGGGTGAGCGCCCAACGTTTTTGGGCGATTGGTACACCACCGA
    ATC
    Clone Rv207
    :::::::::::::::Rv207SP6.seq:::::::::::::::
    CCGCTTCCGTGTAACCGAGCANNGCGAGCGANCTGGCGAGGAAGCAAAGAAGAACTGTTCTGTCAGATAGCTCTTACGCTCAGCGCAAGAAGAAATATCCACCGTG
    GGAAAAACTCCAGGTAGAGGTACACACGCGGATAGCCAATTCAGAGTAATAAACTGTGATAATCAACCCTCATCAATGATGACGAACTATCCCCCGATATCAGGTC
    ACATGACGAAGGGAAAGAGAAGGAAATCAACTGTGACAAACTGCCCTCAAATTTGGCTTCCTTAAAAATTACAGTTCAAAAAGTATGAGAAAATCCATGCAGGCTG
    AAGGAAACAGCAAAACTGTGACAAATTACCCTCAGTAGGTCAGAACAAATGTGACGAACCNCCCTCAAATCTGTGACAGATAACCCTCAGACTATCCTGTCGTCAT
    GGAAGTGATATCGCGGAAGGAAAATACGATNTGAGTCGTCTGGCGGCCTTTCTTTTTCTCAATGTATGAGAGCG
    Clone Rv209
    :::::::::::::::Rv209SP6.seq:::::::::::::::
    TGACACCCAACAGAGGGCACTTAAGATGGCAATGCGGCCGCCTACCTGCACGTTTTCGCGATGTCAGAGGATGCCGAGGGAGAACAATGCGAGCACGGCCGCTGAC
    NTTGCTCACCGCTTTGGCGGCGGTGACATTGGTGGTGGTTGCGGGCTGCNAGGCCCGANTCNAGGCCGAAGCATATAGCGCGGCCGACCGCATTTCGTCTCGACCG
    CAAGCGCGACCTCAGCCGCAGCCGGTGGAGCTACTGCTGCGCGCCATCACGCC
    :::::::::::::::Rv209T7.seq:::::::::::::::
    ACGGGCGACGCTGAGGTGGGCCCGCGGCTATTCATGCTGTCGTCCACGTCCAGCGACGCACTGCGCCAGACGGCCCGCCAACTAGCCACCTGGGTGGAAGAACACC
    AGGACTGCGTGGCGGCCTCGGATCTGGCCTACACGCTGGCGCGTGGCCGCGCGCACCGGCCGGTGCGCACCGCGGTGGTTGCCGCCAACCTGCCGGAGCTCGTCGA
    GGGTTTGCGCGAGGTGGCCGACGGTGACCCCTCTATGACGCGGCGGTGGGACACTGTGATCTAAGACCGGTCTGGGTCTTCTCCGGGCAAGGGTCTCAGTGGGCGG
    CGATGGGCACCCAATTGCTCGCCAGCGAACCAGTGTTCGCGGCCACCATCG
    Clone Rv20
    :::::::::::::::Rv205SP6.seq:::::::::::::::
    ATACTCAAGCTTCGCGAGATCCGGATGGCACTCACGCTGGACAAGACCTTCACAAAATCTGAAATCCTGACCCGATACTTGAACCTGGTCTCGTTCGGCAATAACT
    CGTTCGGCGTGCAGGACGCGGCGCAAACGTACTTCGGCATCAACGCGTCCGACCTGAATTGGCAGCAAGCGGCGCTGCTGGCCGGCATGGTGCAATCGACCAGCAC
    GCTCAACCCGTACACCAACCCCGACGGCGCGCTGGCCCGGCGGAACGTGGTCCTCGACACCATGATCNAAAACTTCCCGGGGAGGCGGAGGCGTTGCGTGCCGCCC
    AGGGCGAACCGCTGGGGGTTCTGCCGCAGCCCAATGATTGCCGCGCGGCTGCATCGCGGGCGGCGACCGCCATTCTTCTGCGAATACGTCCAGGAGTACTGTCTCG
    GGGC
    :::::::::::::::Rv20T7.seq:::::::::::::::
    AGCTTATGTGGCCGCCCACCTACCTTATCTAGCCTAGCTAACTAAATCCAGTGCCGACAGTGCGCGGCTGGCCACCCAGCATGAGGTTATGACCACGGCATATGCC
    AGCGCGCTGGCGGCGATGCCGACGCTGACCGAGTTGGCCGCTAATCACACCAGCCATGCGGTGTTGCTGGGAACGAATTTCTTTGGAATCAATACGATCCCGATCG
    CGCTCAATGAGGCCGACTATGCGCGGATGTGGATTCAGGCGGCCACCACGATGAGTATCTATGAGGGCACCTCCGATGCGGCGCTGGCGTCNGCACCGCAAACCAC
    ACCGGCTCCGGTACTGTTCAACGGCGGTGCTGGCGTTTGCCAGCGCCTGCCGGCGATCTC
    Clone Rv214
    :::::::::::::::Rv214SP6.seq:::::::::::::::
    ATACTCAAGCTTGCCACCCATGCCGAGCAAGGTCGACTCAGCGATGACGAATTGTTCTTCTTCGCGGTGTTGCTGCTGGTTGCGGGCTATGAGAGCACTGCTCATA
    TGATTAGCACNTTGTTTCTGACGCTGGCCGACTATCCAGATCAGCTGACACTCCTTGCGCAGCAACCAGACCTGATCCCGTCGGCGATCGAGGAGCACCTCCGCTT
    TATATCGCAATCCAAAACATCTGCCGCACAACGCGCGTCGACTATTCGGTCGGTCAAGCGGTCATCCCGGGA
    :::::::::::::::Rv214T7.seq:::::::::::::::
    CCGGGGTAGAACGATGCGATCTGGGCCATGTCGACATCGGTGGTACAGGTAAACCGCGCCGTGTGCGCGGTCTCGGAGATCAGAACGTGGTCGCAGTTGACACCGC
    GGGCTTTCAGCCAGTCGCGATAATCGGCGAAGTCGGCGCCTGCCGCCCCAACTAGCGCGACCTCGCCACCTAGCACACCGATGGCGAAGGCCATGTTTCCGGCCAC
    GCCGCCGCGGTGCATCATCAACTC
    Clone Rv215
    :::::::::::::::Rv215SP6.seq:::::::::::::::
    ATACTCAAGCTTGGCGGCAACGCCACTACCGGGCTCACCAGGTCCTGTGCCGCCACCGCCGGCGCCGAAAGCACCATCAGGTCGTAGTTGTCTGGACGTTCGACAC
    CGTAAGCGAACACAATGCCGCCGCCCATGCTGTGCCCGAGCACGATGCGCTTGCACCCGGGATATTCCCGGGTGGCGATCCCAACGAGGGTGTCGAAGTCAGCGGT
    GTATCTGAGATGTCTCTCACTATCATCCGTTTGGCACCCGAGCGGGCATGCCCGCGGGGGGTCAAC
    :::::::::::::::Rv215T7.seq:::::::::::::::
    GTCGACGGCATCAAGGTCCGCAGTGATGGTGTTCATCTCACCCAGGAAGGCGTGAAGTGGCTGATACCGTGGCTTGAGGATTCGGTGCGGGTCGCCAGTTAATCCG
    CCGTGTGCTCCGGATGAGCGCGACGGTAACCCTGGAATTGTGCTGTGTGCTGGCTGTGTCGTTGTGATGAGCCTGTCTAAGTGGTGCGTAACCGTTTGACGAGCCG
    CGGCCTCGCTGCAAACATTGAAGCCCGCACGTCTGGGTTTGTATTTACACAACGAGGGCGCTCCCCGATCTGGCGCGCGCAACGAGGTGCNCACTATCCATTCGAG
    GTGAACTGGACTCCTTGATGCTCATGCCGGTGCGGTTTTGTC
    Clone Rv217
    :::::::::::::::Rv217SP6.seq:::::::::::::::
    ATACTCAAGCTTGCGTTCGATGAAGTAGTCGTCGGTCAGCGCCGCCTCTTCGAGCTCCTTGGCGATGCCCAGCAAGGAGTCATCGCCGCCGAGCTTGGCCAGGATC
    TTGTCGGCCTGTTCCTTGACGATGCGGGCCCGCGGATCGTAGTTCTTGTAGACACGATGACCGAAACCCATCAATTTGACCCCGGCCTCGCGGTTCTTGACCTTGC
    GTTACAAACTCGCTGACGTCGTCGCCGCTGTCGCGAATGCCCTC
    :::::::::::::::Rv217T7.seq:::::::::::::::
    NGTCAAGCCGAGCATGCGCGAGGNAACGACGAACCCAACAAGCCATGGTGGTTGGCGCCGTCGAGAGGTCGGCGGTCGCCACAACGGGAAGATCGCCTTGAGCGTC
    GCTCGACCGCCGCCTCGAGTTGGGTCATAACGAAGTAGCTGATGCCGATCATGTCGACGTTTCCGTCGCATCAGCGTGCAGCGGCGACCCACTCGACGAGGTCTCG
    GTGCCGCCGCGGCCAGGGCACCAGCAGTGACGATTCCAGGCGCCGTCGGG
    Clone Rv218
    :::::::::::::::Rv218SP6.seq:::::::::::::::
    CGATAATCGCTTCCGGTAAGTGCAGCAGCTTTACGACGGCGACTCCCATCGGCAATTTCTATGACACCAGATACTCTTCGACCGAACGCCGGTGTCTGTTGACCAG
    TCAGTAGAAAAGAAGGGATGAGATCTCCCCGTGCGTCCTCAGTAAGCAGCTCCTGGTCGCGTTCATTACCTGACCATACCCGAGAGGTCTTCTCAACACTATCACC
    CCGGAGCACTTCTAGAGTAAACTTCCCATCCCGACCACATATAGGCTAAGGTAATGGGCATTACCGCGAGCCATTACTCCTACGCGCGCAATTAACGAATCCACCA
    TCGGGGCCGCTGGTGTCN
    Clone Rv219
    :::::::::::::::Rv219SP6.seq:::::::::::::::
    NAATAGTCAAGCTTTCTCGTGATTACCACCCGTGTAATTTGGGATGGGCAAAAAGGCGAATCACCGCGTGGCCACAAACGCCGGGAGGGACAATCTCGGGCGGCTA
    GGGCTTCTCGCGGGAAGGCCCGAACGTACGGCGTTTCAACACGTCGCGTCGCCCTCCGACCGCGAACATTCGGGGATGGCAGCAACCTGGTATCACCCTGGCCGGG
    CAATGATCTGCAGCGTCGCCGCGGGTAGTGNCCGCCCGGGCGGCTAC
    :::::::::::::::Rv219T7.seq:::::::::::::::
    CCAACTAGAGCATCGGGACATACGGAGTCAACTACCCGGCCAACGGTGATTTCTTGGCCGCCGCTGACGGCGCGAACGACGCCAGCGACCACATTCAGCAGATGGC
    CAGCGCGTGCCGGGCCACGATGTTGGTGCTCGGCGGCTACTCCCAGGGTGCGGCCGTGATCGACATCGTCACCGCCGCACCACTGCCCGGTCTCGGGTTCACGCAG
    CCGTTGCCGCCCGCAGCGGACGATCACATCGCCGCGATCGCCCTGTTCGGGAATCCCTCGGGGCCGCGCTGGCGGGCTGATGATCGCCCTGACCCCTCAATTCGGG
    TCCAAGA
    Clone Rv21
    :::::::::::::::Rv21SP6.seq:::::::::::::::
    ATACTCAAGCTTGCTGCAGCTTCCTGTGACTGCTCCCGAAACCTGGGGGTGTGCCTGCTGTGTATGCACGGCATACGGACATCCTTCCCCTGAGACCCGCGGTCGA
    ACCAGCCACGTGTCCATCATCAGGGGTCAACCCCGGCCAAGGGCGACGGCACGCCAAGTTCGCCGACCGTTAACCTAGTGCTGTTAGCTTCATTTGCTGCGAGCAA
    AACAGCTGGTCGGCCGTTAGGAACTGAATTGAAACTCAACCGATTTGGTGCCGCCCGTAAGTGTCCTGGCTGCCGGTGCGCTGGTGTT
    :::::::::::::::Rv21T7.seq:::::::::::::::
    AGCTTGCGCGGCGTGGCGATCGCGGTTCAAGGCGCGCTCTTCGAGCACAACGAGCGAAGACAGCTCGGCGACGGAGCCTTTATCGACATCCGTTCGGGCTGGCTGA
    CCGGCGGCGAAGAACTGCTGGACGCGTTGTTGTCGACGGTGCCGTGGCGAGCCGAGCGCCGTCAGATGTACGACCGGGTGGTCGATGTGCCGCGGCTGGTGAGTTT
    TCACGACCTGACCATCGAAGATCCGCCGCATCCGCAGCTGGCGCGGATGCGCC
    Clone Rv220
    :::::::::::::::Rv220SP6.seq:::::::::::::::
    AATACTCAAGCTTGCGCACGACCAGGACGTCGAGTGGCGCTTGCAGTGACTTGGCGACCTCAAAGGCCACCGGTACCCCGCCGCGCGGCAAGCCAAGGACNACNAC
    GGCCTTGCCGGATAGCTGCGCCAGGCGTTGCGCCAACTGGCGTCCAGCGTCGCCACTGATCGTCAAAGAGCTTCATCTGCCGAGTGTGCGCCATCTCATGGCTCCA
    AATATGGAATTAGGTCCCTGGGCCGACTGACGACAGTCCCTCAGCGACCGGATTGCGCATCCCGCCTTGTACGCTGCTCCGCAAATCCCGGGCTTGCGTCCGCGGA
    AGCGAACTCGGCGGCGCTACGGTGGTGGCTCAACTTCGGCCGTGC
    :::::::::::::::Rv220T7.seq:::::::::::::::
    GGTTGGTGCGGTCCACCTTCGCGGCGGCGGCGCGATATGCCTTGCTGGTCTTGCTCATTTGATATCCAATCTATGGGTCGTGGTTACTCAGCGGGCCGAAGCTGGC
    CCTCCCACGGGTAGGGCCCTATTCGACGGTGATGCCCATCGACCGAGCGGTACCGGCGATGATCTTGGCCGCAGCGTCGACGTCGTTGGCGTTGAGGTCCGTCTTC
    TTGGTCTCGGCGATTTCGCGGACTTGATCCCAGGTGACTTTGGCGACCTTGGTCTTGTGCGGCTCCGCCGAACCCTTCGCCACACCAGCGGCCTTAAGCAGCAGCT
    TGGCGGCGGGCGGCGTCTTCAGCGTGAAAGTGAAGCTACGGTCTTCATAAACGGTGATCTCCACCGGGATGACGTTGCCGCGCTGGTTCTCCGTCGCGGCGTTGTA
    CGCCTTGCAGAACTCCATGATGTTGACCCGTGCTGACCGAACGCGGGGCCCACTGGCGGGGC
    Clone Rv221
    :::::::::::::::Rv221SP6.seq:::::::::::::::
    ATACTCAAGCTTTTCGACCCGCAAGCCGGCGGTGCCCCTCCTCGTTCCGCTGCCCGGTCTTCTCGATCGGTTCGGGGTCGCCGCGCTAGGCCCAATTGCCCGGCTC
    CTCCTCGGGCCGTTCCACAACCCGCATCGTCGCCGGGCTAGGTTCAAGCCATGCCGGTAAACCCCAGGACGCCAGTGCTGATCGGCTATGGACAGGTCAACCACCG
    AGGCGACATCGACGCCNAAAATCAGTCCATCGAACCCGTCGACCTGATGGCCNCCGCGGCCCGGAAAGCCGCCGAGTCCACCGTGCTCGAAGCGGTGGATTCCATC
    CGTGTGGTGCACATGCTGTCGGCGCATTACCGGAATTCCCGGGCGTCTCCTCGGC
    :::::::::::::::Rv221T7.seq:::::::::::::::
    NCCTGGTTCATGAACTGGAAGCAGCGCAGCGCTTCCTTTTCGGCCGCAACATGAGCCAGCCTCTCGTCGGCGGTCGGGTGCAGGTGCTCGGGCAGCTCGGCCGCGA
    CAGCCGCCTGACCCTGAAACCAGCTTCCATATCCCGCGACGAACGACGCCAGTCCGCTACGTAACCCCTCCGCGACTGTCCATGGACAACAGCGCGTTCTCCACCG
    ACCGGGGCCGGGTGTTGGGGTGTTCGGCAACGGCAACCAAGTTGGTCCACACTGCCGACGGGGGCCGCAAATCCGTTCACCGAACCAGGCCGCCNAAACAATTCCG
    CCCGATCCCATAT
    Clone Rv222
    :::::::::::::::Rv222SP6.seq:::::::::::::::
    ATACTCAAGCTTGTCGGGATCAATCTCGAGGGCATCCACGCACGAAAAGTAAACTCTATCAAGCTTTTTGACGACACCCACGGACGCCCCATATATGTTCGGGTGG
    GCAAGAACGGTCCCTACCTGGAACGTTTGGTGGCCGGCGACACCGGTGAGCCCACGCCGCAGCGGGCCAACCTCAGCGACTCGATTACCCCGGACGAACTGACTCT
    ACAGGTGGCCGAAGAGCTCTTTGCCACACCGCAACAGGGACGGACTTTGGGCTTGGACCCAGAAACCGGCCACGAAATCTTTGCCAGGGGAAGGCCGGTTTGGGCC
    TTATGTTACCTATATCCTGCCGGAACCTGCGGCTGATGCGGCCGCGGCCGCTCAGGGAN
    :::::::::::::::Rv221T7.seq:::::::::::::::
    AGCAGCTAGCCGCGCTCGCCGCGCTGGTCGGTGCGTGCATGCTCGCAGCCGGATGCACCAACGTGGTCGACGGGACCGCCGTGGCTGCCGACAAATCCGGACCACT
    GCATCAGGATCCGATACCGGTTTCAGCGCTTGAAGGGCTGCTTCTCGACTTGAGCCAGATCAATGCCGCGCTGGGTGCGACATCGATGAAGGTGTGGTTCAACGCC
    AAGGCAATGTGGGACTGGAGCAAGAGCGTGGCCGACAAGAATTGCCTGGGCTATCGACGGTCCAGCACAGGAAAAGGTCTATGCCGGCACCGGGTGGACCGCTATG
    CGCGGCCAACGGCTGGATGACAGCATCGATGACTCCAAGAAACGCGACCACTACGCCATTCAAGCGGTCGTCGGCTTCCCGACCGCACATGATGCCGAAGAATTCT
    ACAGCTCCTCCG
    Clone Rv223
    :::::::::::::::Rv223IS1081N1400.seq:::::::::::::::
    CGCGACTGGCTCCCCGGNCGGCTGCTCGGGTCCGCCGATAGAGACCGGGATGTCGCCCGACGACGGGCAGCCGGGTTGCGTGGGACGGGGCGGGGGTCGGGCAGCC
    CAAGCAACGGGCTAGTCCCCGAATCCTACGGAGCCGTCACCTACGCCTACGTAATAGTAGCTATCAATAACAGTTGACATACGCAACGATCTGTGAGATCAATATT
    GCCTGACGCATGTCAAGACAGGCGTCAAGACAGGTGTCAATAATTCGCTCCGCTGGTGACGGTAACCGGTCGTGCGGGTGTGTGACGCCTAAGGAAGGAGTGTGGG
    TGGTGACGCTGAGAGTGGTTCCTGAGGGTTTGGCGGCCGCCAGTGCGGCGGTGGAGGCGTTGACCGCACGGCTGGCCGCCGCACACGCTGGCGCGGCGCCGGCGAT
    TACGGCGGTGGTGGCGCCCGCGGCGGATCCGGTGTCGTTGCAGAATGCGGTGGGGTTTAGCGCCTTAAGTAGCCAGCATGCCGCGATCGCCGGCGAAAGGGTCCAA
    GAACTGGGT
    :::::::::::::::Rv223SP6.seq:::::::::::::::
    ATACTCAAGCTTATTGAACCGCGGGTCGCAGGCAAAGTGGACCTCATAACGACTCGGGTCCAGCGACCGCGCCAACACGAACGGCCGGACGACGTGGGCCAGGGTC
    GCGGCCTCCCCTACAAACAGGATCCGTTGCCTGCGAACGACAGGCTCCGGTGCGGCGTTGGGCGCCGTGCTCGTCCCAGCGTCCGGTCCCGGGTCGCCGGCGACGC
    TTGTTTCCTCCATACTCGCCCCCTAATCTCGAGGCAGCCCGTACCCGCAGGCAACCTCCCAAAAATGCAATCCCGCAAAATGCAATGCGTCNAGCTATTTCTCACA
    CCGACCGCTAGTTGCGGATCAGAAATCCGTTGGGCGCGGAAGTCCAGCCGAATTTGTTCTCCCGCTCCGCATCATGCTTGTAATCGTTTGGAAATTCATCCTCATA
    TGCCTCGATCGCTTCATAGGGTCCAGGCCCAAACCCGGGCAGGACTGGGTGGCCGTTGATGTTGGAATCCTCCACTACTAGGTATTCACCGGC
    :::::::::::::::Rv223T7.seq:::::::::::::::
    GTCTCGATCATGGCCAAAGAGCTCGACGAAGCCGTAGAGGCGTTTCGGACCCGCCCGCTCGATGCCGGCCCGTATACCTTCCTCGCCGCCGACGCCCTGGTGCTCA
    AGGTGCGCGAGGCAGGCCGCGTCGTCGGGGTGCACACCTTGATCGCCACCGGCGTCAACGCCGAGGGCTACCGAGAGATCCTGGGCATCCAGGTCACCTCCGCCGA
    GGACGGGGCCGGCTGGCTGGCGTTCTTCCGCGACCTGGTCGCCCGCGGCCTGTCCGGGGTCGCGCTGGTCACCGGCGACGCCCACGCCGGCCTGGTGGCCGCGATC
    GGCGCCACCCTGCCCGCAGCGGCCTGGCAGCGCTGCAGAACCCACTACGCAGCCAATCTGATGGCAGCCACCCCGAAGCCCTCCTGGCCGTGGGTGCGCACCCTGC
    TGCACTCCATCTACGACCAGCCCGACGCCGAATCAGTTGTTGCCAATATGATCGGGTTCTCGAC
    Clone Rv224
    :::::::::::::::Rv224SP6.seq:::::::::::::::
    ATACTCAAGCTTTCGTCAGTTCATGGCGCCAGCAGACCAACAAGAGCATCGGGACATACGGAGTCAACTACCCGGCCAACGGTGATTTCTTGGCCGCCGCTGACGG
    CGCGAACGACGCCAGCGACCACATTCAGCAAATGGCCAGCGCGTGCCGGGCCACGAGGTTGGTGCTCGGCGGCTACTCCCAGGGTGCGGCCGTGATCAAGATCTTC
    ACCGCCGCACCACTGCCCGGCCTCGGGTTCACGCATCCGTTTGGCCGCCGCC
    :::::::::::::::Rv224T7.seq:::::::::::::::
    GCCCCGTGTAATTTGGGATGGGCAAAAAGCGAAGCACCGCGTGGCCACGAACGCCGGGAGGGACAATCTCGGGCGGCTAGGGCTTCTCGCGGGAAGGCCCGAACGT
    ACGGCGTTTCAACACGTCGCGTCGCCCTCCGACCGCGAACATTCGGGGATGGCAGCAACCTGGTAGCACCCTGGCCGGGCGATGATCTGCAGCGTCGCCGCGGGTA
    GTCTCCGCCCGGGCCGC
    Clone Rv225
    :::::::::::::::Rv225SP6.seq:::::::::::::::
    ATACTCAAGCTTCCTTTGACCGAACGCGTCCACCGCACCGTGAGATTGGTGGCGCCATTCGTCGTGGTGTAGCTGCTGTTGGCGGCGTCGCCGTATTGTGCGGGCC
    AGCCTTGTGCGGGGGCCGCTTCTACCCACAAGTCGGCACTTCCGCAACCGCCCAGCTCGACCGCGAATTACGGCGGCCGCAACGGCCGCCGGAAGGCGTCACGCAA
    TCGCTTATCCTTTCCAGGTTCCCAAATCCTCCGCTTACTTGGGTCCTTCATCGG
    :::::::::::::::Rv225T7.seq:::::::::::::::
    GGCAGCGGCGACAACCGGAACGTCCGCACGGTGCTCAATCACGGGTGCACGGTGTGCATCAGAATGGCGGGGGTTCGTTGTCGCGGTGAGGCGTTCGGCGAGGAGG
    TAGTGTCTACCCCTTGCCCGCGGGTTCGTGCGGACTGAAAGGGATTTCATTGGGAACCCACGGCTGCGTATCGCAGGGCCTCGGTGACGTCTGCTTCCTCNAGCTC
    AGGAAGTTCGGCGAGAATCTCGGTGGATGTTATTTGGTCCGCCTAC
    Clone Rv226
    :::::::::::::::Rv226SP6.seq:::::::::::::::
    ATACTCAAGCTTTCTCGGCTTCTCTGATAGCCTGAGAAGAAACCCCAAGTTAATCCGCTGCTTCACCTATTCTCCAGCGCCGGGTTATTTTCCTCGCTTCCGGGCT
    GTCATCATTAAACTGTGCAATGGCGATAGCCTTCGTCATTTCATGACCAGCGTTTATGCACTGGTTAAGTGTTTCCATGAGTTTCATTCTGAACATCCTTTATTCA
    TTGTTTTGCGTT
    Clone Rv227
    :::::::::::::::Rv227SP6.seq:::::::::::::::
    ATACTCAAGCTTGGTGACCGGCACCGCGATACGTTGCGGCAGGCATCTGGGCTGGCGGTGGTTCGCCGCTCCGAAGCCGTCGAACACCATCGCCAGCGCGGCTTCC
    ACATCAACGACCATTTCGGCCAGCTTGCGGCGCATCAGCGGCTTGTCGATGAGCGCCCCACCGAATGCCCGCCGCTGCCCGGCGTATCACATCGATTCGACCATCG
    CGCGGCGCGCGTTGCCGAGGGCGAACGAGGCGGTGCCCAACCGCAATCTGTTTGGTCAGCTCCCTCATGCGGGTTGATTCCTTGCCGTCCGGACGGGCCCGCGTCA
    TGCGCTCGGTTCGCC
    :::::::::::::::Rv227T6.seq:::::::::::::::
    CCGTTGCGCAGCGTGAGCCGATAGTTGACATCCGGCTCGGTGAAGGTGAAATCGATGGCCAGGTCGAGGTCCCATGCGCGTGGGCCATTGATGCTGATCGCCAGGA
    CGTCAAAGATTTGGTCCGGCGTCAGCTGGGCGAAAAACGTGGGCGCCGGGACTTGCCCGGAGCTGCCCGGGTTCCCGTCGCGCAGCTCGGCGGCCCCGGTCAGAAA
    GAAATTGCGCCAGGTCGCACACTCCGCGCCGTAGGCCAGCTGCTCCAGGGTGTCGGCATAGAGCCCGCGGGCCGCAGCGTGCTCGCTGTCGGCGAACACCGCATGG
    TCGAGAAGCGTTGCCGCCCAACGGGAAATCACCTGCGTCGAAAGCTTCGCGGGCCAGCTCCAGCACTCGGTCGATGCCACCCAACGCGT
    Clone Rv228
    :::::::::::::::Rv228SP6.seq:::::::::::::::
    ATACTCAAGCTTGCGGATGTTACCCCTGACAGCGTGAACTATGTCNAAACACACGGCACCGGAACGGTGTTGGGGGACCCCATCGAGTTCGAGTCGCTGGCGGCCA
    CTTATGGCCTGGGTAAAGGCCAGGGCGAGAGCCCGTGCGCATTGGGGTCGGTCAAAACCAACATCGGCCACCTGGAGGCGGCCGCCGGTGTGGCTGGATTCATCAA
    GGCGGTGCTGGCGGTGCAACGTGGGCACATTCCCCGCAACTTGCACTTCACCCGGTGGAACCCGGCCATCAACACGTCGGCGACGCGGCTGTTCGTGCCGACCGAA
    AGCGCCCCGTGGCCGGCGGCTGCCGGTCCACGCAGGGCTGCGGTGTCATCGTTCGGCCTCAGCGGGACCAA
    :::::::::::::::Rv228T7.seq:::::::::::::::
    CCGGTAACCAGATCAGCTCGTCGACCTCACTGCCGGGGGTGAATTCCCCACCGGTGCTGCGCGCTGCCCAGTAGTGCACCTTCTTGACGCCTCGAAAAGGGGAGTC
    GGTCGGGTAGGTCACCGTCAGGAGCCGCCTACCCAGGTTGGCGCGGTGACCGGTCTCCTCGAGTATCTCCCGCACCGCCCCCACCGGTGCGGTCTCGGCCGGATCC
    ACTTTGCCCTTGGGCAGCGACCAGTCGTCGTAACGGGGGCGGTGAATGACAGCGATCTCGACCGGCCCTTCCGAATCGGCACTGCCGGGTCGCCAGAACACCGCAC
    CGGCGGCGTACACAATCCGGCCCGCCGAGCGCCGGCGGGCGGACGANTTCTGGATCGACACCTCAACTCCTGCAGGTCAATTCGGCCAAGCTGCTCGCGGTCGTGG
    ATGTGGTC
    Clone Rv229
    :::::::::::::::Rv229SP6.seq:::::::::::::::
    ATACTCAAGCTTGATGCCGCCGAAACCGAGCGTGAGCACGCCGCCACCCACCACGCGCGGGTCGGGCGCCGGGCCCGGGCCGCCAGGCTGCTCCGCTCGGTGATGG
    CACGCCACCGCGACACCACCCGGCTGCGCTACGTCGAGCCATACCGGGCGGAGCTACATCGGCTCGGCCGCCCAGTGTTCGGGCCCTCTTTCGAGGTCGAGGTCTA
    TACCGATTTGCGCATCCGCAGCCGCACCCTGGTCGTCTCGTACCGTGCCCTACCTCTGCTTGTCGGGCGGGGCCA
    :::::::::::::::Rv229T7.seq:::::::::::::::
    TCCGTACGGCCCGGGTACGCTTCGGTCGCAGTGTGCGAGTGATAGATGACGACCGGGACCTCGTCGGCATCTTCCATAGCCCGCCACACCTTCAGTTGCTCACCGG
    AATCCAACCGGTAGAAGGTCGGCGAGCGCTCGGCATTGGTCATCGGGATATGCCGCTCGGGACGGTCAGAGCCCTCGGGTCCGGCCAGCACTCCGCAGGCTTCGTC
    GGGGTGGTCGCGACGCGCATGGGCCACCATCCATCCACCAGGTCTGCGCGAATCACCCGC
    Clone Rv22
    :::::::::::::::Rv22SP6.seq:::::::::::::::
    GGACACATTGCGAACATTGATGACAAAATAGAAATCATTGATGGTTTGAGTCACCAGGCCGATCAAGCCTTCGCCGAGCCAAATTCCAATCAAGAGGCCCAAGCCC
    GTACCAATCAGCCCGGCAACGAGGGATTCCGTCATTATCAGCCAAAATAACTGCTCTCGGGTTACACCCAAACAGCGCAATATGGCGAAAAACGGTCGCCGTTGCA
    CGACATTAAATGTCACGGTATTGTAAATTAAAAAGATACCCACCAACAAGGCAATCAAACTGAGAGCGGTTAAATTGACCGTAAAAGCGTCCGTCATCTGTTTGAC
    GGTGTCCCGTTGGGTNTCCGACGTTTCCATACGCACACCGGCCGGCAGTCTTTGTTGGATGCGTGTTGCAGTGGCCTCATCTTTGATGATCA
    :::::::::::::::Rv22T7.seq:::::::::::::::
    GCCTGGCCCAGGTGAAGGCCGACCTCGACGCCAAAGCCGCTGATCCGGCACATGAGTCGGTGGACTGGGACTTGAAGTCGCTGCGATGGGCGTGGAACCGAGCCAA
    AGATGACGTGGCGCCGTGGTGGGCCGAGAATTCCAAGGAGTGCTACTCGTCGGGGTTGGCCGATCTGGCCCAGGGCCTGGCTAATTGGAAAGCTGGCAAGAACGGG
    ACCCGCAAAGGCCGGCGGGTGGGCTTCCCGCGATTCAAATCCGGGCGGCGTGATCCTGGCAGGGTGCGGTTCACCACCGGCACCATGCGCATAGAGGATGACCGGC
    GCACGATCACGGTCCCGGTGATCGGGCCGCTGCGGGCCAAGGAGAACACCCGCCGGGTGCAACGCCACCTCGTGAGCGGGCGCGCGCAGATCCTGAACATGACCTT
    GTCGCAGCGGTGGGG
    Clone Rv230
    :::::::::::::::Rv230SP6.seq:::::::::::::::
    TAACTCAAGCTTCAAGTCCGCNGTCCGACCCTGTTCGACGGCTACCTGAATCAACCCGATGCCCCGCCGCGGCGTTCGACCCGACAGCTGGTACCGCACCGGCGAC
    GTCGCGGTGGTCGACGGCAGTGGGATGCACCGCATCGTGGGACGCGAGTCGGTCGACTTGATCAAGTCGGGTGGATACCGGGTCGGCGCCGGTGAAATTGAAACGG
    TGCTGCTCGGGCATCCGGACGTGGCGGANGCGGCAGTCGTCGGGGTGCTCGACTATTATCTAGGCCAGCGGATCGTTGCCTACGTAGTCGGCTCAGCGAATGTCGA
    TGCGGACGGGCTTATCAACTTTGTTGCCCAACAACTTT
    :::::::::::::::Rv230T7.seq:::::::::::::::
    CCATGTCGCCCCAACATATCGTCGATGTTCGCGTCGTCCGCCTCGCGCACGTGGTCTGTCACCAGTCAACGTTAACGCCGCCGCACATGTCCTGCGGCCGGGCAAA
    ACGTGAAAAACGAGCGGGCGACTGCAATGTCATGACACCGACGCCGCCGATGGGCCCAGGGTCTGGCAGATTCGATCTGTGCGGCCAGTGCCAGCAGCGTCGCCTC
    GTCATACGGCCGGCCGACGAGTTGAACCGACATGGGCATGCCGTCGCCGTCGAAGTCCCACCGCACCACGGCCGCGGGCTGGCCGGTCAGATTCCANACTTGAAAG
    TACTGAAGCCGCTGCACCACCAG
    Clone Rv231
    :::::::::::::::Rv231SP6.seq:::::::::::::::
    CGAAAGCGTGAAACAGCTCGCGGCAGCCCCCGACGTGCTGCGTCGGATAGCCGGCGGGCGAAGATCAATTCCAGGCAGCTCCCGGACAATGCGGCTCTGCTGGCCC
    GCAACGAAGGACTCGAGGTCACCCCGGTGCCCGGGGTCGTGGTGCACCTGCCGATCGCACAGGTTGGCCCACAACCGGCCGCTTGATGCCCGGTCGGCAAGCCCGG
    CAGTTGCCAAACCCAGCGTGATCNTGCTCNGCTCTNTANTTCGGCGAAGAAGTGGCTCGCCTGATCACCTACCATCGGCCAGGATCTGCGTGTCATCACAACGCTC
    GCCAAGGAGGTTGTTGTG
    :::::::::::::::Rv231T7.seq:::::::::::::::
    TCCGCCACGCTTCGCGCCGCCCGGCATACGGCGCGTACCGATCTCCCGCGTCATACACCGCGGGTAATCGCCGACGGTGCGGTTCGGGAGCCGAAGGTGACGACGC
    TGATTGAATCGAGTTCCAGGTCCAGCGGGTGGCGCAGCAACGGCGCGAGCTCAACGACGTCAATCACGTTGTCGCTTTCTACGGTCACCGACCCGGTGACCGTAGT
    CGCCCGGTGCGCTCGGCCGAGAAGCTGCACCGCCACCACCGCGACACCGTCTTGCACGCGGACCCACCCCGGATCGGTTGTTGGCCAAGGTAATTGGGTCATTCCA
    TTTGACGGGACGCCGACCC
    Clone Rv232
    :::::::::::::::Rv232SP6.seq:::::::::::::::
    CATTCTTTAACAGTTGTTTTGGGCTCGGCATGGTTAGCCAACGTTCTGCGGTCCACCATATCATCTTGGTCCGGTAGCGCTCGTCCGGGGTATGCTGCCGCCGGGA
    TTCTCGCTGCTATTACTCCCCCCGAAGAACCGCCACCGGTCCAGCGCGTGGGCCGNCGCGGTCCCATCACAAACTGAACCCCCAACAGGGACATGCTTATCGGTAG
    GGCGCGCGCCAAGGCGGCAGCAATCGCATCACTGCGCTCTGCGCGTCACTATTAACCCACCCGGACTTCACTTCCACCACCCCGAATGGCGCCCGGTCATTGATCA
    TCTGGCGCACCGCGGATAA
    :::::::::::::::Rv232T7.seq:::::::::::::::
    CGGTGTCCTGCAGTTGGTAGGCCTGCAGTTTGTGCATCATGCCGATGCCGCGGCCTCGTGGCCACGCATGTACAGCACCACGCCGCGCCCCTCACGGGCGAACATC
    GCCAGCGCGGCGTCCAGCTGAAGCCCGCAATCGCAGCGGCGTGACCAAACACATCGCCGGTCAAGCACTCCGAATGCACCGGACCAGCACGTCGTCACCGTCGGCG
    TTGGGCCCGGCGATCTCGCCGCGGACCATGCGCGACATGTTCCACGTCCTCGTANATGCTGGTGTAGCCGATGGCGCGAAACTCCCCATGACGAGTCGGAATCCGC
    GCCTCGGCGACCCGCTCAATGTGCT
    Clone Rv233
    :::::::::::::::Rv233SP6.seq:::::::::::::::
    CGGCATCTGGCGGCTGAACCTGTTCTTGGGCAACATGCCGAGGATCGCCTCTTCCACCACGCGGTCGGGGTGGCGTTGCATTACCTCACCGATGGTGCGCTTGTGC
    AGGCCGCCGGGATACCCCGAGTGCCGGTAAACCATCTTGTGCTGCAGTTTGTCGCCGCTGATGGCGACCTTGTCGGCGTTGATCACGATNACNAATCACCGCCANC
    GACATTGGGGGCGAACGTCGGCTCGTGCTTGCCGCGCAGCAGGCTGGCCGCCGCGACGCAAGGCGCCAACCACCACGTCCGTGGCGTCGATGACGTACCACCATCG
    CGTGGTGTCACCCGCCTTGGGC
    :::::::::::::::Rv233T7.seq:::::::::::::::
    GCGGCAAAAATTGAAGCACTCNTGGCCACTNCCGCCGGGAGGGACAATCTCGGGCGGCTAGGGCTTCTCGCGGGAAGGCCCGAACGTACTGCGTTTCAACACGTCG
    CGTCGCCCTCCGACCGCGAACATTCTGGGATGGCAGCAACCTGTTAGCACCCTGGCCGGGCGATGATCTGCAGCGTCGCCGCGGGTAGTCGCCCCCGGGCGGCTAC
    AGTCTGAAACGCGATGACCATCGATGTGTGGACGCCGCATCCGACNCAACGGTTCCTACACTGTGATATGTTCGCCTCGCTGCGCCGGTGGACGGTGGGTCTATCC
    CGGA
    Clone Rv234
    :::::::::::::::Rv234SP6.seq:::::::::::::::
    CGCGTTGAACTGAAGGGGTGCCGCCCGGCTCGAGCAGGCAAGCCATTTGTTCGATGCGGTTACCGAAGATCTCTTCGGTGACTGCCCGCCGCCGGCCAGCTCGGCT
    CAGTGTCCGGCGTTGGTCGCCGCGGCGACAATCTTGGCGTCCACGGTGGTCGGGGTCATGCCCGCGAGCAGGATTGGCGAGCGGNCGGTCAGCCGGGTGAACTTCG
    TCAAGAGCTGACGCTGCGGTTGGGGAGGCGAATCATGGTCGGTGCGTAGCCTCGACTAGGCCCGGG
    :::::::::::::::Rv234T7.seq:::::::::::::::
    TGACAACGCGGCGGCGATTACCCCGCTACCGCAGCAGCATGACGCGGTAGCGAACACCGCCGGATGCAGCGCAGGTGCGTCGATGTGCTCACGGAATCGCCCCGGC
    ACCGCGATCTCGAGGATCACCAGTGCCACCCCCTGCAGCGCGACACCGACGATTCCGTACACCGCCACGCCGATCAGGCCCTGGGCCAGCTGATTGGAGCTGGCGT
    ATATGGCGGCGATGGTGACGATGGTCATCGCCTCTTACATTGTGGCGGCCAGAACCACGGCGTTGGGGCGGCGGTCGATGAACACTAGGCGACCANATCCCCGGGG
    TCAACAGGTTGACCATCC
    Clone Rv235
    :::::::::::::::Rv235SP6.seq:::::::::::::::
    CGCGGACATCCCGAACGAGGACACGCGACCGCTTCGGTGTGTGATCTATCAGGGCTCGCACCACGCGCAACCGCTTCCGGCTACCTAGACGCGGT
    :::::::::::::::Rv235T7.seq:::::::::::::::
    GCATGCGGGTGATGCCGTTCTCAGTGCGCAACAGCGTTCGACGCGGCATACCCAGCCGCACATGCCGTGCACGCCCGAGCCGGGGCGGGAATCT
    Clone Rv237
    :::::::::::::::Rv237SP6.seq:::::::::::::::
    CTCAAGCTTCAGNCCNTCTAAGCGGTCTGCGCGGCGATCGCAAAGATCGCCCTTTGCCGGCGTTGGGGGCTTCTGCTCGGGGGTGTTGTACACCTTCTCGAACACC
    TCGGCACCGACACCACCACCGTCGGCTTGAACACCGCCAACATCGGCAGCANATCTTGATGTCCTGGTGAATCCACGGTGACTTTGGAGTGGAAGGCGGCCATACT
    GATCGCGCGCGCCACCACATGAGCTAGCGGCAGGAAAACCAGCAGCCGCTCACCCTTGCGCAGCAGCGTCGGGTGATATGCCTGGCGCCC
    :::::::::::::::Rv237T7.seq:::::::::::::::
    AGTCGAANGTCAGTCCGGTCTCCTCTCCGACTACGGCCAAGAACTGGGGCGACGGTGTCAGTGCAGAACAGCGGAAACTGGTGGCGCCCTAGGCGAGCGAACGCTC
    ACAAACGGCGGTGACCGCTTCTGGTCGTGCACCATCGAGCCGTGCCCAGCCCGGCCGCGTGCCGTCAGCCGCATCCACTGGATGCCCTTCTCGGCGGTTTCAATCA
    NGTACAGGCGACGTTCGCCACCATCGTGCCGGGGCACGTTAGCGAGAAACGCCGACTTCACCGATTGCCTCGGTGATGxxxxx
    Clone Rv23
    :::::::::::::::Rv23T7.seq:::::::::::::::
    AGCTTCGCGGCGTGGCGATCGCGGTTCAAGGCGCGCTCTTCGAGCACAACGAGCGAAGACAGCTCGGCGACGGAGCCTTTATCGACATCCGTTCGGGCTGGCTGAC
    CGGCGGCGAAGAACTGCTGGACGCGTTGTTGTCGACGGTGCCGTGGCGAGCCGAGCGCCGTCAGATGTNCGACCGGGTGGTCGATGTGCCGCGGCTGGTGAGTTTT
    CACGAGGTGACCATCGAAGATCCGCCGCATCCGCAGCTGGCGCGGATGCGCCGGCGGCTCAACGACATCTACGGCGGCGAACTGGGTGAGCCCTTCACCACCGCCG
    GGCTGTGCTACTACCGCGACGGCTCTGACAGGGTCGCCTGGCATGGCGACACCATTGGTCGCGGCAGCACTGAGGACACTATGGTGGCGATCGTCAGCCTCGGCGC
    CACCCGCGTCTTCGCGCTGCGGCCGCGTGG
    Clone Rv240
    :::::::::::::::Rv240SP6.seq:::::::::::::::
    AGCTTCAGCTGATACTCGACCAGCCCCACTCGGGCCAATACGTGAATGTCTAGCATCTTCACCCGTTCACGGGCTANTCGAGTAGTAGACATTGATTAGCCTGAAC
    GTACCTCCGACGCCAGCTGACGAACGGGTATGCGGATGGATTTCGTGGTGTCGCGCCCGAAGGTCAATTCGTTACGGATGTATCTCGGGGCCGGATCGGGGCCGAT
    GTTGGCGGCCGCGGCGGCCTGGGACGGACTATCCGACGAACTGGCGGTGGCGGCGTCGTGGTTTGGGTCGGTGACCTCGGGGCCTGGCGGATGCGGCGTGCGCGGC
    CCGCGGCGGTTGCGATGGCNCGCGCGGT
    :::::::::::::::Rv240T7.seq:::::::::::::::
    CTGGTCATGGACGTTGCTCCGGTAGTGGCTCACTGCCGATCCTCCTCGTTGAGAGTGCCACCTCAGGGTTGGGTAGGGTTGGGTACTCGAAACCAAGTTACCCACC
    AGTAACACCGTCAAAATATATCCGTTGCATAGGTCAATGCAAGTTGATGTGAGCTACATTGCACCAACTAACTAACCAACCGGTTGGGTTAGCGGTGATCCTGGCC
    GTGTCGGTCCTCTCACCTGCGGTGATAGCGATCAAATGAAGAATATGCGGAGTCTAGGGCGGCAGCGCCTGGCANCGTAGATCATCGGCTCACGCGGATGCGGCCT
    CTTGGTACGGACATGCGCGCG
    Clone Rv241
    :::::::::::::::Rv241SP6.seq:::::::::::::::
    CTCGTGAGTAGCACCCCTGTAATTTGGGATCGGCAAAAAGGCGAATCACCGCGTGGCCACGACACGCCGGGAGGGACNATCTCGGGCGGCTAGGGCTTCTCGCGGG
    AAGGCCCGAACGTACGGCGTTTCAACACGTCGCGTCGCCCTCCGACCGCGAACATTCGGGGATGGCAGCAACCTGG
    :::::::::::::::Rv241T7.seq:::::::::::::::
    GGATCAACTACCGGCCAACGGTGATTCTTGGGCGCCGCTGACGCGCAACGACCCAGCGACACATTCAGCAGATTGGCCAGCGCGTGCCGGGCCACGATGTTGGTGC
    TCGGCGGCTACTCCCATGGTGCGGCNCGTGATCGACATCGTCACCGCCGCACCACTGCCGGCCTCGGGTTCACGCAGCCGTTGCCGCCCGCAGCGGACGATCACAT
    C
    Clone Rv243
    :::::::::::::::Rv243SP6.seq:::::::::::::::
    AGGACCGTCAGCACGGCGACGTGCTACTCGCCGAGCAGTGGGAATCGCTCTGCAGCAAACCATTACTCTGCGCGACGTTCGAGATGACCTTCTGAATGGACGGATC
    TACCTGCCGCGCGACGACCTGGACCGCGTATGCGTCCGCCTCCGCCTGGACGACACCGGGGCACTCTATGACCCCGACGGACGGCTCGCGGTACTGCTGCGGTTCA
    CCGCCGAGCCCCGCACGGTACGCGTCGGGACTGCGCTGAGTCCANCCTCGACGCCGTAGCGCTGCTGCTGTGCGGCCATGTCTGGCATCTACCGCCGTCGCTCCCT
    TGA
    :::::::::::::::Rv243T7.seq:::::::::::::::
    CGACTCTGTTGGCCACTGCGGGTCGATCTTGCGGCCGCCCCGGTCGTGGAACGCCCAGGTCACCCGGCGGCGCACCGCGGTCAGCGCGTCGTTGGCCAGCGTGGTC
    ACATGGAAGTGGTCGACGACGAGCTTGGCGTTGGGCAGCAGCCCGGGCGTGCGGATCGCCGAGGCGTATGCAGCGGCGGGGTCGATGGCCACCGTACTGGATGCTC
    TCCCGGAACTGCGGTGTGCGCGCTTGCAGCCATGCCAGCACCGCCGCGCCGCCGCGGCCTTCATGCTGCCCATAAACCCTGATACCGGCCAGGTCGACNAACCNGT
    ATCCCACGGTCAACCC
    Clone Rv244
    :::::::::::::::Rv244SP6.seq:::::::::::::::
    CACACGGACGGCGGTGCGGACGCAGCTGACGCGCATGGTGGTCAGCATCGCGGCCGGTCTGCTGTTGTATGCCTACTTCGCGCCGCGCAAATGCTGGTGGGCGGCG
    GTGGTGGCGCTCGCATGGCTGGGCTGGGTGCTGACCCAACTCTCGAACCACACCCGGTGGGTGGGCTGGGCTATGGCCTGCCATATCGGCCTGGTGTTCTACN
    :::::::::::::::Rv244T7.seq:::::::::::::::
    CCGATATCCGAGCCGATAGCTGGCGGGCTCGGGTGGTNGCCAGCGGCGCTGCGACGAAAGTGTGACCGTCATGAAACAGACACCACCGGCGGCCGTCGGCCGTCGT
    CACCTGCTCGAGATCTCAGCATCCGCAGCCGGTGTGATCGCGCTTTCGGCGTGTAGTGGGTCGCCGCCCGAGCCCGGCAAACGCCGGCCCGACACAACCCCGGAAC
    AGGAAGTCCGGTCACCGCGCC
    Clone Rv245
    :::::::::::::::Rv245SP6.seq:::::::::::::::
    GCTTCAGGACAAATTGNATCCCTATGCACCCGTTGTCACGCCGATGAGTGAAGACTGCACCGCAATCGCCGGAATCCGGCAAACCCTGCACAAGCGAAATCAACCG
    GAGGCTGACAAGGCAACGTCGGTGATCCGTACCGCCTGGTTGGACAAACGGCAGAAGGCGCCTCGTCCGGTCCATCTACGCCGAGCACACTGGTGATAGCGCCATC
    GGCATCGGTGCGGCCACGGTGGAGACGAACGTCCGCNGGCGTCTGGGTCAGTAACCCGCCGACCAGTTCTCGGGCAAGCTGGTCAACATCGGGCGCCACGTCTCCA
    AC
    :::::::::::::::Rv245T7.seq:::::::::::::::
    GTTTGGCGGCCTTATTGCACTGAGGTCGTCAATTGACCCACAGCGGAAATGCCGACTATTCGCAGGCCTCCTTGGCCTTGGCTGCCGGAGATGGGCTCCGCGGGAA
    CCGCATGCAGGTATATGACCTCGGTTTCTCGGGTGCTACCGCGTGCCTTGTCGAGGATGAACTCGGCGTTGGAATTGTCCAGCCGGCCCAATTCATCGAGCGCAGA
    TTCGTACACATGGCCGGCGGCGACATACCTTCACCGTGGATCTGCTCCACACGGACCGCCCTGTCGGGATCTGCTCACGGGTAAAGGAATTA
    Clone Rv246
    :::::::::::::::Rv246SP6.seq:::::::::::::::
    GCGCACTCCTCCTTATCGCTCCGCTCTGCATCGTCGCGGCGCGGTCAGGTGCAAACGCCTTCGGGGGTGGGGGTCCTGCGGAGCACACCGGATACGGAGCGCAACG
    CGTCGCGTTGTGCGGGCAAACAAGTGTGCAGGNNCCAATGCCATGTCCAGCAGCTTATCAGTGTCGAACGTGCGAACGTCGCGCCTTCGCCGGTGCCTGAATCTCT
    ACAAG
    :::::::::::::::Rv246T7.seq:::::::::::::::
    CGCTGAAAGCCACCATTCGCCGGGTCGGCGCCGGGCTCGGGCCGCCAGGCTGCTCCGCTCGGTGATGGCACGCCACCGCGACACCACCCGGCTGCGCTACGTCGAG
    CCATACCGGGCGGAGCTACATCGGCTCGGCCGCCTAGTGTTCGGGNCCTCTTTCGAGGTCGAGGTCGA
    Clone Rv247
    :::::::::::::::Rv247SP6.seq:::::::::::::::
    TGTAATTTGGGATGGGCAAAAAGCAAANCACCGCGTGGCCACAAACGCGGGGAGGGACAATCTCGGGCGGCTAGGGCTTCTCGCGGGAAGCCCGAAACGTACGGCG
    TTTCAACACGTCGCGTCGCCTCCGACGCCGAAATTCGGG
    :::::::::::::::Rv247T7.seq:::::::::::::::
    CTTGGGCAACATGCTGAGGATCGCCTTTTCACCACGCGGTCGGGGTGGCGTTGCATTAGCTCACCGATGGTGCGCTTGTTGCAGGCCGCCGGGATACCCGAGTGCC
    GGTAAACCATCTTGTGCTGCAGTTTGTCCCGCTGATGGCGACCTTGTCGCGTTGATCACGATGACGAAGTCACCGCCATCGACATTGGGGGCGAACTCGGCTTGTG
    CTTG
    Clone Rv249
    :::::::::::::::Rv249SP6.seq:::::::::::::::
    GCATGCTTCATTATCTAATCTCCAGCCGTGGTTTAATCAGACGATCGAAAATTCATGCAGACGGTCCCAAATAGAAAGACATTCTCCAGGCACCAGTTGAAGAGGT
    TGATCAATGGTCTGTTCAAAAACAAGTTCTCATCCGGATTGAACTTTACCAACTTCATCCGTTTCATGTACAACATTTTTAGAANCATGCTTC
    Clone Rv24
    :::::::::::::::Rv24SP6.seq:::::::::::::::
    ATACTCAAGCCTGATGCCGCCGAAACCGAGCGTGAGCACGCCGCCAGCCACCACGCGCGGGTCGGGCGCCGGGCCCGGGCCGCCAGGCTGCTCCGCTCGGTGATGG
    CACGCCACCGCGACACCACCCGGCTGCGCTACGTCTATCCATACCGGGCGGAGCTACATCGGCTCGGCCGCCCATTGTTCNGGCCCTCTTTCGAGGTCGAGGTCTA
    TACCGATTTGCGCATCCG
    :::::::::::::::Rv24T7.seq:::::::::::::::
    TCCGTACTGGTCGGGTACGCTTCGGTCGCAGTGTGCGAGTGATAGATGACGACCGGGACCTCGTCGGCATCTTCCATAGCCCGCCACACCTTCAGTTGCTCACCGG
    AATCCAACCGGTAGAAGGTCGGCGAGCGCTCGGCATTGGTCATCGGGATATGCCGCTCGGGACGGTCAGAACCTCGGGTCCG
    Clone Rv251
    :::::::::::::::Rv251SP6.seq:::::::::::::::
    GTTCTCGCACGATTTCGGATTAGCGGGATGGTCTCAATTGGGTATGCGGGGAAGGCGCTGACATTCGCCGCGATTAGCTGTTTGATGGACCGGGGGTGATTTTTGA
    TCACGGAAATGGGTGTTTATNCAGGTCGCACGCTTTCATCCGGGGCGGAACG
    :::::::::::::::Rv251T7.seq:::::::::::::::
    GGGTGTGCCTGCTGTGTATGCACGGCATACGGACATCCTTCCCCTGAAGACCCGCGGTCGAACAGCCACGTGTCCATCATCANGGGGTCAACCCCGGCCAAGGGCG
    ACGGCACGCCAAGTTCGCCGACCGTTAACCTAGTGCTGTTAGCTTCATTTGCTGCGAGCAAAACAGCTGGTCGGNCGTTAGGAATGAATTGAAACTCAACCGATTT
    GGTGCCGCCGTAGGTGTCCTGGCTG
    Clone Rv252
    :::::::::::::::Rv252T7.seq:::::::::::::::
    ACTACCCGGCCAACGGTGATNTCTTGGCCGCCGCTGACNGCGCGAACGACGCCAGCGACCACATTCAGCAGATGGCCAGCGCGTGCCGGGCCACGANGTTGGTGCT
    CGGCGGCTACTCCCANGGTGCGGNCGTGATCGACATCNTCACCGCCGCACCACTGCCCGGCCTCGGGTTCACCAGCCGTTGCCGCCCGCAGCGGACGATCACATCG
    CTTTTATTTNNTNTTCNGGAATCCCTCGGGCCGCGCTGGCGGGCTGATGA
    Clone Rv253
    :::::::::::::::Rv253SP6.seq:::::::::::::::
    ACGTCGGGANACTGTTCGCGTTCATCCTCGTCTCGGCGGATTGGTCTGCTGCGCCGGACCGACCGATCTTCAGCGGGGGGTCACGCTCCGTGGGGTGCCGTTACTT
    CCGATCGCCCAGTGTGCGCGTGCTGTGGCTGATGCTGAACCTCACCGCGTTGANTTGGATCGGTTCGGGATCTGGCTGGTGGCCGGAACGCNATTTATGTCGCTAC
    GGGCGCCGGC
    :::::::::::::::Rv253T7.seq:::::::::::::::
    GCTCAAAGGCACTACTGGCACCAAGGCCCACACGTCACCTGTGACTCCTGCGCCGACCCGCCCGAGGTCTGGCCGTTACACCGAACGGGCGAGCCGGGAGTTGGTA
    CCATCGAACAAGACAAGGTGCATGGGCGGAGTTGTTCCGCCACTTCGTCGATGACGGGTC
    Clone Rv254
    :::::::::::::::Rv254SP6.seq:::::::::::::::
    CGATACCGGCTGCTTACCGAGACATCCACCATGCCACCCGAATCACCGCACGCGCCGAAATCGCACAACAGCTTGACGCCTTGCAGGTTCCGCGATTGGAATTGCC
    GACGGTCTCTGACGGCGTCGACCTTGGCAGCCTCTACGAGCTCTCGGAATCACTTGCCCAGCAGGGGGTTCGATGAGTGTCACACCGAAGACCTCGATATGGGCGC
    AATCCTGGCCGACACATCCAACCGGGTGGTTGTGTGCTGCGGCGCCGGTGGGGTCNGCAANACACTACCGCGGCCGCGCTGGCGTTGCGCGCGGCCGAATATGGCC
    GCACTGTGGTCG
    :::::::::::::::Rv254T7.seq:::::::::::::::
    CGTCGTCGTCGTGGTATGCGATAGCCATCCCGTCGGGCTACTCGCCATCACCGATCAGCTTCGCCCCGAAGCCGCCGCGGCGATTTCCGCTGCGACCAAACTGACC
    GGGGCCAAACCGGTATTGCTTACCGGCGACAACCGGGCCACCGCCGATCGGCTCGGTGTACANGTTGGCATCGACGACGTACGGGCCGGGCTACTGCCGACGACAA
    NGTCGCAGCCGTGCNGCNGCTGCAAGCTGGAGGTGCCAGATTGACCGTGGTCGGTGACGGTATCAACGACCTCCGGCCTTAGCGGCCGCGCATGTCGCATCGCCAT
    GGGCAGCGCCCGAC
    Clone Rv255
    :::::::::::::::Rv255SP6.seq:::::::::::::::
    GCACGCAATCGAAGTCACCCAAACCGGGCGGGCCAGGCGTCTNACGCCACGTCNACCAGCCGCAACCTCAACCCGGCCACGGCGAGCTCCTGATCAAGGCCGAGGC
    CATCGGTGTCTACTTCATCGACACCTACTTCCGCTCCGGGCAATATCCGCGCGAACTCCCGTTCGTCATCTGCTCCGAAGTATGCGGCACGGTGGANGCCGTCGGC
    CAGGGGTTAC
    :::::::::::::::Rv255T7.seq:::::::::::::::
    TCGACTGTGTGGCCACAGATCACGCCCCGCATGCCGAGCACGAGAAATGCGTCGAATTCGCCGCGGGCCGGCCGGCATGCTCGGGTTGCAGACGGCATTGTCGGTG
    GTGGTGCATACAATGGTGGCGCCGGCTTGTTGANTTNGGCGCGATATCGCGCGGGTGATGAGTGANAACCGGCGTGCA
    Clone Rv257
    :::::::::::::::Rv257SP6.seq:::::::::::::::
    GAACCTGACACCCTGGTCACGGGTGAGCACGGACTTGATTTCTTCNCTATTGGTCGGCGCTGTTGAGCACACCACGCCGCTGACGGCCGTCGCGTCCTCGCTGTGC
    TCGGTCTGGTGGAGCGCGCTGCCCGCGGCCNAACATCNTAAATCAAGCGTATTCGTCAACAGATATCATCAATGTCGGCGCTGGACTATTCAAATCATCGATATAC
    TGGTGACCTGGTCCTTCGCCATCGATCAATGGCGATAGTCACGCAAATCGTCACGGACATCGTCGGCGTCCCAGCTGGCCCGTGCCAACAGATGCTGCAACCCATC
    GGGGTGGTATCACCGCGGTGCTCGGCGATGGTCCACAATTCTTGCGGTCCAAGCCCNAAACATCCCGGGCATGAATTCACCGGCATGCGCN
    :::::::::::::::Rv257T.seq:::::::::::::::
    CTATCGTACCCGCGCCGGTCACCTTCTGGATATCGCCGGCCTGGTCAAGGGGGCGTCCGAGGGAGCCGGGCTGGGTNACAAGTTCCTGGCTCATATCCGCGAATGC
    GACGCCATTTGTCAGGTGGTGCGGGTGTTCGTCGACGACGACGTGACTCATGTCACCGGACGGGTCGATCCCCAGTCCGACATTGAGGTCGTCGAGACCGAGCTGA
    TCCTGGCAGATCTGCAAACCCTGGAGCGGGCCACGGGCCGGCTGGAGAATGAAGCGCGCACCAACAAGGCGCGCAAGCCGGTCTACGAAGCGGCACTGCGTGCCCA
    GCANGTGCTCGACGCCGGGCAAGACGCTGTTCGCCGCGGGGGTGGATGCCGCCGCGTTGCGCGACTGAAACTGCTGACCACCAAGCCCTTCCTGT
    Clone Rv258
    :::::::::::::::Rv258SP6.seq:::::::::::::::
    TACTCAAGCTTCAGGCCGCCACGTCCGCCGTCCGTCGGCGACGTGACCTCGAGCGCCGAGTTCGACTCGACATCGCCGCCGGCGCATGCCGACATGAACGCGGCAC
    TCACCGCAAGCCCGTCGGACGTCAGGTCGATCGACTCCGCTTCAAGCACCGGATCGTCCGGGCAACTCGCGGCCTCGGCCTGTGCGAACGGCACACCCGTCGTGGC
    GGCNCCCCGCGCGGAACTGGGCTCATCACGGTCGTTGCGAGCCGGTCGCGTCACCGCGTACCGACGCCGTC
    :::::::::::::::Rv258T7.seq:::::::::::::::
    CCGACATCGAGTGGGCTCGCAGTGACTTGGCGACCTCCAAGCCACCGGTACCCGCCGCGCGGCAAGCCAAGGACGACGACGGCCTTGCCGGATAGCTGCGCCAGGC
    GTTGCGCCAACTGGCGTCCAGCGTCGCCACGATCGTCAAAGAGCTTCATCTGCCGAGTGTGTCGCCATCTCATGGCTCCAAATATGGAATTAGGTCCCTGGGCCGA
    CTGACGACAGTCCCTCAGCGACCGGATTGCGCATCCCGCCTTGTACGCTACTCCGCAAATCCCGGGCTTGCGTCCGCGGAAGCGAACTCGGCGGCGCTACGTGGTG
    GTTCACTTCGGCCGTGCGCACTCGGATCGACGGGCCGATGGTGGCCGGGCCCGCGCGCTTCTTGGTCATCCGATTGAGT
    Clone Rv259
    :::::::::::::::Rv259SP6.seq:::::::::::::::
    ATACTCAAGCTTGTCGCGGTAAACCGCACGCAGGGCGGTGGGTGCGGTGTCAAAGACACCCACACTTCTTTGCGGTTCGGTGATCTCGACACCGGCCGCGAGCCGA
    CCACCATGCGCGCGTAGATCGGCGATCAGCGCGTCGGCTATCGCCTGGGTGCCGCCCACCGGAATCGGCCAGCCGACCGAATGGGCCAGCGTTGCCAGCATCAGTC
    CGGCGCCGGCCGACACCAGTGACGGCAACGGTGAAATCGCGTGGGCGGCAACGCCGGTGAACAACGCGCGGGCATCCTCGCCCGCCAACGACCGCCACCGAGGGTG
    CCTGGGCCATCATCCGCAGCCCGA
    :::::::::::::::Rv259T7.seq:::::::::::::::
    TGGACTCATAACGATCGGGTCAGCGACGCGCCAACACGAACGGCCGGACGAGTGGGCCAGGGTCGCGCCTCCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAG
    GCTCCGGTGCGGCGTTGGGCGCCGTGCTCGTCCCAGCGTCCGGTCCCGGGTCGCCGGCGACGCTTGTTTCCTCCATACTCGCCCCCTAATCTCGAGGCAGCCCGTA
    CCCGCAGGCAACCTCCCAAAAATGCAATCCCCCAAAATGCAATGCGTCGAGCTATTTCTCACACCGACCGCTAGTTGCGGATCAGAAATCCGTTGGGCGCGGAAGT
    CCAGCCGAATTTGTTCTCCCGCTCCGCATCATGCTTGTAATCGTTTGGAAATCATCCTCATATGCCTCGATCGCTTCATAGGTCAAGGCCAAACCCGGCAGGATGG
    GTGGCC
    Clone Rv25
    :::::::::::::::Rv25SP6.seq:::::::::::::::
    CTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATTTAGGTG
    ACACTATAGAATACTCAAGCTTAGTGGTTGCGCACGTAAATTCGTCAGGTGACCGATCCCCTGCTGTCTCACTCGCCTCACAGCGACCACCACGGCTGGCGCTCAA
    GGCGGGCACGTGCGGAGCAGATGAGGAATGTGCGACGTCTTGATGCAGCCTGTCAGAACACCGAGACCCTCGACGAACTTACGATCGAAACCGCTTAGGCCAACCG
    GTGACGGGGGTGTCTTTCCGCGGCTAGGGCGCCTTATCGTCCGAAGGCCGTGGGTGGTGATCGCCTTCTGGGTCGCGCTTGCGGGTCTGCTTGCGCCGACGGTGCC
    GTCCCTGGACCGATCTCCCAGCGGCATCCAGTGGCGATTCTGCCATCGG
    :::::::::::::::Rv25T7.seq:::::::::::::::
    CAGGCATGCAAGCTTGCGATGTATCAACACGCCGTTGCGCAGCGTGAGCCGATAGTTGACATCCGGCTCGGTGAAGGTGAAATCGATGGCCAGGTCGAGGTCCCAT
    GCGCGTGGGCCATTGATGCTGATCGCCAGGACGTCAAAGATTTGGTCCGGCGTCAGCTGGGCGAAAAACGTGGGCGCCGGGACTTGCCCGGAGCTGCCCGGGTTCC
    CGTCGCGCAGCTCGGCGGCCCCGGTCAGAAAGAAATTGCGCCAGGTCGCACACTCCGCGCCGTAGGCCAGCTGCTCCACGGTGTCGGCATATAGCCCGCCGGCCGC
    AGCGTGCTCGCTGTCGGCGAACACCGCATGGTCGAGAAGCGTTGCCGCCCAACGGAAATCACTGCGTCAAAGCTTCGCCGGGCCACTCCAGCACTCCGTC
    Clone Rv260
    :::::::::::::::Rv260SP6.seq:::::::::::::::
    ATACTCAAGCTTGACCGACGCTGATCGCACCGCACGCGGGAACCTCAAGGGCACTACTGGCACAAGGGCCCACACGTCAACCTGTTAACTCCTGCGCCGACCCCGG
    CCGAAGTCCTTGGCGTTAACACCGAACGGGCCAACCCGGGAATTTGGGTTCCATCAAAACAAATAGCAGGTGCCTGGGCGGAGTGTTC
    :::::::::::::::Rv260T7.seq:::::::::::::::
    GTCGTCGTGTGCTGGGGCGTCCGTATCAGCACGCCCACGAAATGGGGCACAAGAAGGATTCCTGGAACGGTGGCTGTCCAAGATCACCCTCGCCCAAAACTGCTAC
    GGGCACTTCTACATCGAGCACAACCGTGGCCATCACGTCCGCGGTGTCCACACCGGGAGG
    Clone Rv261
    :::::::::::::::Rv261SP6w.seq:::::::::::::::
    ATATGCCTTGCTGAGCTTTTCGGATCGCAGCGAGTCGTACCCGCGCCGGTCACCTTCGTGGATATCGCCGGCCTGGTCAAGGGGGCGTCCGAGGGAGCCGGGCTGG
    GTAACAAGTTCCTGGCTCATATCCGCGAATGCGACGCCATTTGTCAGGTGGTGCGGGTGTTCGTCGACAACGACGTGACTCATGTCACCGGACGGGTCGATCCCCA
    GTCCGACATTGAGGTCGTCGAGACCGAGCTGATCCTGGCAGATCTGCAAGCCCTGGAGCGGGCCACGGGGCGGCTNGAA
    :::::::::::::::Rv261T7.seq:::::::::::::::
    GACACCCTGGTCACGGGTGAGCAGGACTCGATTTCTTCGCTATTGGTCGGCGCTGTTGAGGCACAGCACGCCGCTGAGGCCGTCGCGTCCTCGCTGTGCTCGGTCT
    GGTGGAGCGCGCTGCCCGCGGCCGAACATCGTAAATCAAGCGTATTCGTCAACAGATATCATCAATGTCGGCGCTGGACTATTCAAATCATCGATATACTGGTGAC
    CTGGTCCTTCGGCCATCGATCAATGGCGATAGTCACGCAGATCGTCACGGCATCGTCTGCGTCCCAGCTGGCCCGTGCCAACAGATGCTGCACCCCATCGGGGTGG
    TATCNCCGCGGTGCTCGGCGATGGTCCAACAATTCTTGCGGTCCAAGCCCGAAACCATCCGGCCATGAGTTCACCGGCATGGCGCAACGGCTGGTGCCGGGCAAAA
    CGCGGCGCGATCGAATTC
    Clone Rv262
    :::::::::::::::Rv262SP6.seq:::::::::::::::
    TGTAGAAGGTGGGTCCCGTCCAACTTCGCGGCGGCGGCGCGATATGCCTTGCTGGTCTTGCTCATTTGATATCCAATCTATGGGTCGTGGTTACTCAACGGGCCGA
    AGCTGGCCCTCCCACGGGTAGGGTCCTATTCGACGGTGATGTCC
    :::::::::::::::Rv262T7.seq:::::::::::::::
    CCCGAATCCGGTGGCCGGCAGGGGGCCTGGCGACGTGGACACCTTCTAACTTGTCTTTACCGGTCACTGTTGCACCCCAACACCTTTAACGACGTGGACGGACGTT
    ACATCGGATTCGACGGTGTCATCCACAGCGTTGCCATTGGGCACACCCACTACGCCAATTTCTCCGACTGGGACACCTACCGCAGCCTCGCCCCACTGCAGGGACT
    GTTGTTCCCGCAACGGGCCATCGACATGATCCAGTCGTTGGTGACCGACGCGGAGCAGACTGGTGCGTATCCGCGTTGGGCGCTGGCGAAATTCCGCCACCGGCAT
    GAT
    Clone Rv263
    :::::::::::::::Rv263SP6.seq:::::::::::::::
    TTGAGATGCTGGTCGGGATGCCGATGGTTGGAACATGGTCCCCTGGCGTCGAATACGCGCGAGCGCATGAGCTCACCGGTTCGGAACAACGTATCGAAGAACTCGC
    ACTGCTGGCAGATGGTATCTCCGATGTGGTTGTAATTTGTATCCCAACTCTAACTGTGCTATCGGATCTGCGTGAATA
    :::::::::::::::Rv263T7.seq:::::::::::::::
    CGTAATCACGATCCCGCTGAGACACTTGACCTTACGGCCGAAGTGACTTCGCTGCTGCTATGCCGACACCCGATTTCCATACGCTGCTGTACACGACGGCCGGGCC
    GGTGGCCTCCATCACGCTCAACCGCCCGGAACAGCTCAACACCATCGTCCCGCCCATGCCCGACGAGATCGAGGCCGCTATCGGGTTGGTCGAACGCGACCAGGAC
    ATCAAGGTCATCNTNCTGCGCGGTGGCGGGCGCGCCTTCTCCGGCGG
    Clone Rv264
    :::::::::::::::Rv264SP6.seq:::::::::::::::
    CAAGCTTAAGCTGGTTCCGGCCACTCCATGAGCCGTAGTGCAATGGTTCGTGCACGGCGAGGCCGAACTTGCCATAAACATCCCTGACGAAAGTCTCCGGCAAGCC
    GATTGCTTCTTCGGGCCGCTTCTTGTGGATTGTCCGATAACCCGGTCCCTCATGCTGGAAGTTGTGCGCACTCTTTCCTTCCGCGATGTGGGCTAACGACTCGTCA
    TTGAGCAAGAAGTACGTGCACAGGCATCGTCCGCCGGGCTTCAGCACGCGGGAGATCTCGTCCAGATAGTGCTCCACGTCCGGNGGGAAACATGTGGGTGAACACC
    GAGGTNAGAAACACCNCATCCAACGACGCATCCGGGATATGGAAAGCGAAA
    :::::::::::::::Rv264T7.seq:::::::::::::::
    TATGGTCTTCGTCGACCAGTACGTCGTAGGCGCCATGAGCCAGCGACTGAAGCCGCGCCATGCCTGCACGGCCCGCTCATCCAGCGAGGCGGCCATCTCCCGCAGA
    TAGCCTGCCGCCTCGGCGCGCACGCTGTCCGGATCGCGTCCGAGCTCGTCGGCCAGCGCACGCAGCCGCTCGTCATACCATCGGGCATCCAGCAGTTGGGTAACCT
    CAACGGGGTCGGTCGCTAGCGGCGTCATTGATTCAGCAACAATACCGATGCGCTGCAGCAACTTTCGCAGTCCGATGCGGCCCACCTCCCGTGCAGTCACTGGCTA
    GCCCCCGTCATGCCGGTTGTGTCGATGGCACGGCAGCGGGCTCGTAAACCTGCGGTCTCAGCTCGCTGG
    Clone Rv265
    :::::::::::::::Rv265SP6.seq:::::::::::::::
    GCTTAGCGGTCTTGCTCGAACCGACATTGCGTGCCACTCATGAGCGGGTGGCGGTCGCGGTGCTTACACATCT
    :::::::::::::::Rv265T7.seq:::::::::::::::
    GTATCTGGCGCCTCTCGAATATCCTTGAACGTCCCGCGGTGCCACCCAGATAGATCGCAGCGCCCTGCAATGGAGTTCCCTTTATGGCCTCTCTAGCCTCCCGCTT
    GATCGGCTCGACCCGAGAGATGCCCTCGGGCGTTGCGGGATCTCCCTCCA
    Clone Rv266
    :::::::::::::::Rv266SP6.seq:::::::::::::::
    CTTCACGCCGATCCGCGACCGCGAACGCGACGGTGACGGTGGCGACAAGGTTCGGTTGGTCGCCGCGGCGCTGGGCGATATCAGCTCACCCGGTTTCGAAGGTGTT
    CGGCGACCGGACGGTGCTGCAGACATTCTTGAGCGTCCTCGACCGGCCCGATTCGGCCTTCAACATCGTGACGCCGTATTTCGGCGGTACCGCTCGGCGCCGAGTC
    GAAGGCGGCCTGAGCTAAAGCCGGGCATTGCGCGAGTGGTAAACAAGTTCGGTGACTTCGGTTGACCGACTCGACGGGCTCGATCTGGGCGCGCTGGACCGGTATC
    TGCGTTCGCTGGGGATCGGGCCNACCGCNANTTGCGTTGCGANCTGATTCCGGTGGAGCTCCAATCTGACTTCCGG
    :::::::::::::::Rv266T7.seq:::::::::::::::
    GCAGCTACCGACCCTAGCGACGAGTGTGTTCGCAGCGTCGAATGTGAACGTTCGGCGTGATTCGGCGCGCGGGTTCCCGCTCTCAGCGCACGTTCGGCGCCGAGGN
    GGCTAGTCCCTGGTTAAGCAATGTCTCGGTCGCCGCCAGCAGCGCGCATGTCGCCAACCCGTCNACCGCGTTGCGCATGTCCGGTACCGACGGAAACGACGGCGCG
    ATCCGGATGTTCTTGTCGTCCGGATCCTTTCGATACGGGAACGACCCCCCGCCTCGGTCACCGCGATACCAACGTCCTTAGCCAANGCTACNGTCCGGCGCGCGGT
    CCCGGGCAACACGTCGAAGCTGATGAANTAACCACCCTTGGGCTCGGTCCAAGANGCGATCTTGGACTCCTTAACCGCTGATNCAA
    Clone Rv267
    :::::::::::::::Rv267IS1081N60.seq:::::::::::::::
    TCCCCATCGGCGCCGGACCGTTTGAAAGTCCAAGCACGGGTGGGATGGAATCGACGACAGTTGAGCGCCGTCGGTGGCCGTGGTCAGCAGCTGTTCGCGAACGCAC
    CAGGTCACATCCCTTCGACATCTCACCGACGTGGCACGGGCGACATCAACAGGAAGATTGACGAATCCCTCGCAGGCGCGGCACGTCCGCAGGCCAACGCCAACTA
    CGGGGCCACCAGCGATCCTCCGCTCACGCACCAGCCCAAGCCAGGCTCANCCACCCAAGTCGGCCCGCGCTCTCCCTGGCCCCCTGGTCTCCGGGGCCTTGTTAAA
    CAACTACCGGAAGTCCACCAATCCTCGCTGCATCTCGACACCGTCCGCCTCACTCCCTTCCTCCCGCCCCTCTCCACACNACACACCTCTTGCATTAAGGTCACGG
    AGCGGTCACTTTTCGTCGGACGAAATTCGCAATCCGGCCGCTCGCCGCCAGAGAT
    :::::::::::::::Rv267SP6.seq:::::::::::::::
    CGGAAAGTGGATACTCCCAGCAGGTAGCAGGTCGCCACCACGCTGGTCAGTGCGCGTTCAGCTCGCTTGCGGCGCTGCAGCAGCCAGTCCGGGAAATAGCTGCCCT
    GGCGCAGCTTGGGGATCGCGACGTCGATGGTTGCGGCACGGGTGTCGCAAATCACGGTGGCGGTAGCCGTTGCGCTGATTGGACCGCTCATCGCTGCGTTCGCGGT
    AGCCCGCCCCGCACAGGGCGTCGGCTTCAGCCCCCATCAGGCGGCGA
    :::::::::::::::Rv267T7.seq:::::::::::::::
    GGCCGAGTCCAGCACTTCGCACTATGTGCAGACCAAANACCCGGTGGTCGCCGCGCTGCGGCAGCGGCTGGCAACGGCGCCGGTGATCACCGAGTGGTGCGNAGTT
    GCCGACCGGCAGTTCGCCGCGGGCTTACTACGAGAAGGGCCTGCGCGACGTCATCAGGTATCACGTGTCGATGACGTCGAGCGTTAACTTCCCCGACCAGACGGCG
    ACCTCGCCGATGGACCCCGCGTTGTACCTGGTGTGGGCGCAAGCTAACGCCGCCGCANGCTATCGGTACTCGGTCGAAGCGCAGCCGGGGTCGCAAGCGCTAGCGG
    GCAAGGTCGCGACGATCTCGGTCACCTGGACCAACTACGGCGCTGCTGCCGCCACCGAATAGTGNGTGCCCGGCTACCGGCTGGTGGATTCCACGGGACATGTGGT
    TCGGACCTGCCGGCAGCGGTGGAACTGAAGANGCTGGTCT
    Clone Rv268
    :::::::::::::::Rv268SP6.seq:::::::::::::::
    AGCTTCAAGGACATCGTCATCGCGACCAAAACCGCGAGCTAGGTCGGCATCCGGGAAGCATCGCGACACCGTGGCGCCGAGCGCCGCTGCCGGCAGGCCGATTAGG
    CGGGCAGATTAGCCCGCCCGCGGCTCCCGGCTCCGATTACGGCGCCCCGAATGGCGTCACCGGCTGGTAACCACGCTTGCGCGCCTGGCGGCGGCCTGCCGGATCA
    GGTGGTATATGCCGACAAAGCCTGCGTGATCGGTCATCACCAACGGTGACAGCAGCCGGTTGTGCACCATCGCNAACGCCACCCCGGTCTCCGGGTCTGTCAN
    :::::::::::::::Rv268T7.seq:::::::::::::::
    GCTCGCGGTCCAGCAGCAGACGTGTCTGACCCCGACGCCCGGCCGCCGGTACCGAAACCGGATCGGCCCGCCGATGGCCGCGGCCACGGCGTCTGCCTTACCCGGC
    CCGGATACCAGCAGCCACACCTCGCGGGAACGCTGAATCGCCGGCAGGGTCAAGGTGATTCGGCGTGGCGGCGGTTTCGCGAATCGTCCACCGCCACCACCATGCG
    GGTGCTCTCGAAGACGCGGGGCTGTGCGGGAACAGCGAGTTAATGTGGCCCTCGGGCCCCATGCCCAGCAGGTGGACGTCGAAATTCGGCCCGGGTCACCTGGTGC
    GGCACTGGCGGCC
    Clone Rv269
    :::::::::::::::Rv269SP6.seq:::::::::::::::
    AGCTTGTCGATCGTCCGGCAGCGTCCGGCGAGTCAAGTCGAAGCCAGTCCGGTCTCCTCTCCGACTACGGCCAAGAACTGGGCGACGGTGTCAGTGCATACCAGCG
    GANACTGGTGGCGCCCTAGGCGAGCGACCGCCTCACAAACGGCGGTGACCGCGTTCTGGTCGTGCACCATCGAGCCGTGCCCATCCCGGCCGCGTGCCGTCAGCCG
    CATCCACTGGATGCCCTTCTCGGCGGTTTCAATCAGGTACAGGCGACGTTCGCCANCATCGTGCCGGGGCANGG
    :::::::::::::::Rv269T7.seq:::::::::::::::
    TTGGTGATCATCGNCCCAACGACCCCGAGGCGATGTTCTTGCACACCGAGGAGTGTCGCAAGCTGGGGCTGGCCTTCGCCGCCGATCCGTCTCAGCAGCTGGCGAA
    GCTGTCGGGGTGAGGAAATTCGCAGGCTCGTCAACGGTGCTGCTTACTTGTTCACCAACGACTACTAATGGGATCTGCTGCTGTCCAAGACCGGCTGGTCAGANGC
    CGATGTGATGGCGCAGATCGACCTGCGGGTGACCACATTGGGTCCTAAGGGTGTCGATTTGGTAGAACCTGACGCACCACCATCCACGTCGGCGTTGGTCCCCGAA
    ACAGCCAGACCGA
    Clone Rv26
    :::::::::::::::Rv26SP6.seq:::::::::::::::
    GGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACACTATAGAATACTCAAG
    CTTGATTTTGATCATCATGATGATCATCACCCGAAGTGTGGTAGCCGCAGTGGTTATCGTGGGTACCGTCGTGCTTTCCATGGGCGCCTCTTTGGGGCTTTGCGTA
    TTGGTCTGGCAGGACATTCTGGGTATCGAGTTGTACTGGATGGTGTTGGCGATGTCGGTGATCCTGCTCCTGGCGGTGGGATCCGACTACAATCTGCTGCTGATTT
    CCCGGTTGAAAGAGGAAATTGGGGCCGGATTGAACACCGGAATTATCCGTGCCATGGCTGGTACCGGGGGAGTGGTGACGGCTGCCGGCATGGTGTTCGCCGTTAC
    CATGTCGTTGTTTGTGTTCAGCGATTTGCGAATT
    :::::::::::::::Rv26T7.seq:::::::::::::::
    CAGGCATGCAAGCTTGGCGTGCCGTTCCAACCCGAATTGGCTTTCGGCGCCATCGGTGAGGACGGCGTGCGGGTGCTCAACGACGAGGTCGTCCGCGGGACACACC
    TCGATGCTGCCGCCATGGACGCGGTCGAACGCAAGCAGCTGATCGAGCTACAACGCCGCGCGGAACGCTTCCGCCGCGGGCGTGACCGCATCCCGTTGACCGGGCG
    GATCGCGGTGATCGTCGATGACGGCATCGCCACCGGAGCGACGGCCAAGGCGGCGTGCCAGGTCNCCCGGGCGCACG
    Clone Rv270
    :::::::::::::::Rv270SP6.seq:::::::::::::::
    GGCATCTTGGCCGCCATGTTAGCCACACTGCCACCGGCTATAGAAGCGATGCGCACCGTCCTGCCAGCACATTGCGGCGCTCCTCCCTGGAAAGCAAGATAACCAA
    GCTCATGCCGTGGTTGTGGGTGGCGTGGTTTGGTTTGGGTAACTTTGG
    :::::::::::::::Rv270T7.seq:::::::::::::::
    TCGGCTAATAATCGTCGACGCCGGCCTCCTCTGCAATCGCCTTGGCGGTCGCCGGGTTGTCACCGGTGATCATCACGGTGCGGATGCTCATTCGGCGCATTTCGTC
    GAATCGTTCCCGTATGCCCACCTTGACGATGTCCTTCAGATGGACGACGCCGATGGCCCGCGCGCTGCTGTTATCGGTCCATTCCGCAACGACTAGGGGTGTCCCC
    CGCCGGAGCTGATGCCGTCGACAATGGCACCCACCTCCTCGGTGGGGTGGGCACCGTGATCGCGAACCCACTTCATCACCGCAGCCGCGGCACCTTGCGGATTCGA
    CGGATG
    Clone Rv271
    :::::::::::::::Rv271SP6.seq:::::::::::::::
    CTCAAGCTTGGAGGCGTGGCGATCGCGGTCCAAGGCGCGCTCTCCGAGCACAACGAGCGAAGACNGCTCGGCGACGGAGCCTTTATCGACNTCCGTTCGGGCTGGC
    TGACGGCGGCNAAATAATGCTGGACTCGTTGTTGTCGACGGTGCCGTGGCGAGCCGAGCGCCGTCAGATGTACGACCGGGTGGTCTATGTGCCGCGGTTGGTGAGT
    TTCCACGACCTGACCATCGAAGATCCGCCGCATCCGCTGCTGGCGCGGATGCGCCGGTGGCTCAACTAATTCTACGGCGGCGAACTGGGTNATCCCTTCNCCACCG
    TCGG
    :::::::::::::::Rv271T7.seq:::::::::::::::
    CCTAGGTCAACCGTACCGTCATCGGATCGGGGTCGACCGCACAGATGGACTGGAGCTTCGGCGAGGTCATCGCCTATGCCTCGCGGGGGGTGACGCTGACCCCGGG
    TGACGTGTTCGGCTCGGGCACGGTGCCCACCTGCACGCTCGTCGAAGCACCTCAGGCCACCGGAAATCATTCCCGGGCTGGCTGCACGACTGCGACGTGGTCACCC
    TCCAGGTCGAAGGGCTGGGCGAGACGATGCAGCCGTCCGGACGAGCGGCACTCCTTTTCCGTTGGCTCTTTCGGCCGAATCCGGACGCCGAACCCGACCGGCGCGG
    GGTCAACCCGGCACCGACGCGGGTGCCGTTTACCCGCGGGCTGCACAAATCCCGACGGGTATGGGCTTTGACCTGCCGACGGGGGA
    Clone Rv272
    :::::::::::::::Rv272SP6.seq:::::::::::::::
    AGCTTGGCGTGACACCAACACAGGGCACTTAAGATGGCAATGCGCCGCCTACCTGCACGTTTTCGCGATGTCAGAGGATGCCGAGGGGAGAACAATGCGAGCACGG
    CCGCTGACGTTGCTCACCGCTTTGGCGGCGGTGACATTGGTGGTGGTTGCGGGCTGCGAGGCCCGAGTCTAGGCCGAAGCATATAGCGCGGCCGACCGCATTTCGT
    CTCGACCGCAAGCGCGACCTCAGCCGCAGCCGGTGGAGCTACTGCTGCGCGCCATCACGCCGCCTAGGGCTCCGGCGGCGTCGCCGAACGTCGGGTTTGGCGAACT
    GCCTACCCGGGTCCGGCAGGCAACCGAT
    :::::::::::::::Rv272T7.seq:::::::::::::::
    TCATGCCGTTGGACCGACCATCGGAGTTAGTTGCCGAACCGCGGGACCACCGCAAGCACCCGGTCCTGGTCGCGCACCGCGTCGGCCAACCGCTTGAGCACCACCA
    CGCCGCAGCCCTCGCCGCGCACGAATCCATCCGCGTTGGCGTCGAAGCTGTTGCATCGGCCGGTCGGTGACAGCGCCGACCACTTGGACAGCGCGATGGCGGTGAA
    CGGTGACAAGGTGAGCTGCACCCCGCCCGCCAATGCCACGTCGGTTTCACGCAGGCGAAGCTCTGACACGCCAAGTGAATTGCCACCAGCGACGACGAACAAGCGG
    TATCTACGGCGATGG
    Clone Rv273
    :::::::::::::::Rv273SP6.seq:::::::::::::::
    GGGTCGACTTTCTGCAAGGCGAGGCTACACCGTCGTCGTCGTGGTATGCGATAGCCATCCCGTCGGGCTACTCGCCATCACCGATCAGCTTCGCCCCGAAGCCGCC
    GTGGTGATTTCCGCTGCGACCAAACTGAACGGGGCCAAACCGGTATTGCTTACCGGCGACAACCGGGCCACCGCCGATCGGCTCGGTGTTCAGGTTGGCAT
    :::::::::::::::Rv273T7.seq:::::::::::::::
    AATCCGAAATCCTGACCGATACTTGAACCTGGTCTCGTTCGGCAATAACTCGTCGGCGTGCAGGACGCGGCGCAAACGTACTTCGGCATCAACGCGTCCGACCTGA
    ATTGGCAGCAAGCGGCGCTGCTGGCCGGCATGGTGCAATCTAACAGCACGCTCTTCCCGTACACCAACCCCGACGGCGCGCTGGCCCGGGCGGAACGTGGTCCTCG
    ACACCATGATCGAAAAACCTTCCCGGGGAGGCGGATGC
    Clone Rv274
    :::::::::::::::Rv274SP6.seq:::::::::::::::
    TTCCGAATTTCGGGTCCNGGTCATATGACCCTCATGGAAGAAGAAGCGGCCGCCCCGCGCCCGTGCGACGGCGAATGAAAACCCTCACCCAGGCCGCATTGAACGC
    CGACAAGACGGTGGAGCAGGTCGAAGACGTCCTGGACGGTCTGGGTAAGACCATGGCCGAGCTGAACAGCTCGCTGTCACAGCTGAACAGCACCGTGGAGCGCTTG
    GAGGACGGTCTGGACCATCTCGAAGGTACCCTGCACAGCCTGGACGATCTCGCGAAACGGCTCATCGTGTTGGTCGAGCCGGTGGAAGCCATCGTCGATCGGATCG
    ACTACATCGTGAGCCTCGGCGAAACGGTGATGTCACCGCTGTCGGTC
    :::::::::::::::Rv274T7.seq:::::::::::::::
    NCTCGATCTTGGGGTACGTTCGATGAGGCTGCTGACCAACAACCCGGCCAAGCGGGTGGGACTGGATGGATACGGATTGCACATCATCGAGCGCGTGCCGCTGCCG
    GTGCGGGCCAACGCGGAAGAACATCCGTTACCTGATGACCAAGCGTGACAAATTGGGGCACGACTTGGCTGGGTTGGACGATTTTCACGAATCCGTGCATCTGCCC
    GGAGAATTCGGCGGTGCCTTGTGAAGGTGGCGCCGGGGTGCCGGATCTGCCGTCGCTGGATCGTCTGGTGTGCGGCTGGCGATTGTCGCCAGCAGCTGGCACGGAA
    AGATCTGCGACGCGCTGTTGGACGGCGCCCGCAAGTGGCCGCCGGTGTGGCCTCGATGACCGACTGTGGTCTCGGGTGCTCCGCGCGATCGATAT
    Clone Rv275
    :::::::::::::::Rv275SP6.seq:::::::::::::::
    TCATCCCGACCAAAACGCGAGCTAGGTCGGCATCCGGGAAGCATCGCGACACCGTGGCGCCGAGCGCGCTGCCGGCAGGCCGATTAGGCGGGCATATTATCCCGCC
    GCGGCTCCCGGCTCCGAGTACGGCGCCCCGAATGGCGTCACCGGCTGGTAACCGCTCTTGCGCGCCTGGGCGGCGGCCTGCCGGATCAGGTGGTAGATGCCNACAA
    AGCCTGCGTGATCGGTCATCACCAACGGTGACAGCAGCCGGTTGTGCACCAAGCGCGAACGCCACCCCGGTCTCCGGGTCTGTCCAACCGATCGACCGCCCAAGCC
    CACATGAACAAACCCCGGCATCACGTTGCCGATCGGCATACCGTGA
    :::::::::::::::Rv275T7.seq:::::::::::::::
    TTGGCGGGTTGGCCCAGCAGCCCGCCGGTGACGGCGACGATGCTGGGCTGGTTGCGGCCCTGCGCCACCGCGGCTTGCATGCTGGTTGGCTGTCTTGGGACGATCC
    CGAAATAGTCCACGCGGATCTGGTGATTTTGCGGGCTACCCGCGATTACCCCGCGCGGCTCGACGAGTTTTTGGCCTGGACTACCCGCGTGGCCAATCTGCTGAAC
    TCGCGGCCGGTGGTGGCCTGGAATGTCGAGCGCCGTTACCTACGTGACCTGATGGATCGGGGGGTGCCGACCGTGCCCGGCGATGTGTATGTGCCGGGANAGCCGG
    TCCGGTTGCCACGCAAAGGCCATGTCTTCGTCGGTCCGACCATCGGTACCGGGACACGGCGCTGTATTGCCCGGTTCGCTGCCGAGTTCGTCGCGCAACTGCACGC
    NGGCGGGCCAGCGGTGCTCGTTCANCCCGGAGGTTCCGGTGACGATGATCGTGTTGGTCTCCCT
    Clone Rv276
    :::::::::::::::Rv276SP6.seq:::::::::::::::
    GTAGGAGAGAACAAAGACCGTCGATAGGACACGTGTTACGCCGGTAGCTGTCATTGGTATGGGGTGCCGCTGCCGGGGGGCATCTACTCACCCGATCGGTTGTGGG
    AGGCGTTGCTGCGGGGCGACAATCTGGTCACCGAGATCCCCGCCGACCGCTGGGACATCTACGAGTACTACGACCCCGAACCCGGCGTGCCCGGACGCACCGACTG
    CAAATGGGGCGCGTACCTCGATAACGTCGGCGACTTTGATCCCGAGTTCTTCGGGATCGGGGAGAAAGAAACGATAGCGATCGATCCGCAGCACCGCTTGTTGCTG
    GAAACCTCCTGGGAAGCCATGGAACACGGCGGGCTAACACCGAACCATATGCCTCCCGACAMGGGTTTTCGTGGGGTT
    :::::::::::::::Rv276T7.seq:::::::::::::::
    CGAACTGAGCCCATAGAAAGGCAGCGACTAATTCGCTGGGCAAATAGGAAGACCCTTTGTCCTGCCACGTATATTTGTCGACCTCGTTGCGAAGGAAGCGGCTGCG
    ATTGGTGCCCTTTTCCCTGGAGAATCTCTGCCCGGAGCAGGAAGTCTTATGAGTTGACAAGCAGGGGCGCCGCCTTCGCCGGAAATCACATTCTTGGTCTCGTGAA
    ATGAGAGCGCTCCCAGGTCGCCGATGCTGCCGAGCGCCCGCCCACGATACGACGCCATCGCGCCTTGGGCCGCGTCTTCGACCACCGCCAGGTTGTGGTGCGTGGC
    GATCTTCATGATCGCGTCCATCTCGCAGGCCACCCGGCATAGTGAACGGGGACCATGGCCTCGGTTCGCGGGTGAA
    Clone Rv277
    :::::::::::::::Rv277SP6.seq:::::::::::::::
    CTTAGACGCCACCTCCGGGCCGAGCTCCACGGGGTGGATAAGTACGGCCGGATGTGGCCGCAATGGGAAGTTGTTGCCCGCTTGACTGTCCGGGTTAACGCCGGAT
    TCCACCACATCCCCTTGCGAAAGGCCGTTGGGTT
    :::::::::::::::Rv277T7.seq:::::::::::::::
    GATCGCGATCGTCGATGTGGCCATCCGGCTTGGCGTCGACCCGCGTAAGGCAGACCAGATGGTTCGCGGCACGGTCAACCTGCCACACGCACTGGTAAGACTGCCC
    GCGTCGCGGTATTCGCGGTTGGTGAAAAGGCCGATGCTGCCGTTGCCGCGGGGGCTGATGCTGTCGGATCGACGATCTGATCGAGAGGATCAGGGCGGCTGGCTGG
    AATTCGATGCCGCGATCGCGATACCGGATT
    Clone Rv278
    :::::::::::::::Rv278SP6.seq:::::::::::::::
    AGCTTACGCCGCTTTCGCTTCNGATTTGGGACGCCGCATCGAAAGCGCAGTTGGAAGCGCGGCGCCCGGCTGGTCGAGCTGCTCAAGCAGCCGCAATCCCAGCCCA
    TGCCCGTTGAGGAGCAAGTGGTTTCGATCTTCCTGGGCACCGGCGGTCACCTGGACTCGGTGCCCGTCAAGGATGTCGGCGGTTCGAAACCGAATTACTGGACCAC
    ATGCGGGC
    :::::::::::::::Rv278T7.seq:::::::::::::::
    CGACGGGACCTCGTCGCATCTTCCATAGCCCGCCACACCTTCAGTTGCTCACCGGAATCCAACCGGTATAAGGTCGGCGAAGCGCTCGGCATTGGTCATCGGGATA
    TGCCGCTCGGGACGGTCAGATGCCCTCGGGTCCNGCCAGCACTCCTCAGGCTTCGTCGGGGTGGTCGCGACCGCATGGGCCACATCGCATTCACCAGGTCTGCGCG
    AATCACCAGCACGTANACGGTTCCTTTCCTAAGCAACACCGAAATTTCAGGACCCGAATGCTCCGGGAAAACATGTCACGGTAAGTCCGGTATTCCGGGTACCGGT
    TGAGCATTGA
    Clone Rv279
    :::::::::::::::Rv279SP6.seq:::::::::::::::
    CGGCATCGGTTTGGGCTGTCACCAGCAGTTGGTACTTCTTCACTACTGTTGTTCGAGCGTCGAGCCGCCGCGCGTGTCGAGGTCGCCGGACGCGTACCCGCCAGGC
    CGGTCAGGGTGCCCTTCCAGTCCACGCNGCTGTGGTCGGCTAACCGCTTATCTTCAATCGAGACNATCGCCAGCTTCATCGTGTTGGCGATCTTGTCCGAGGGCAC
    CTCGAACCGGCGCTGCGANTACAGCCACGCGATCGTGTTGCCCTTCGCGTCGACCATCGTCGATACCGCAGGCACTTGCCCCTCGAGCAGCTGGGCCGATCCGTTG
    GCAACGACCTCAGAGGCACGATTGGACATCAGCCCTAGCCCGCCTGCG
    :::::::::::::::Rv279T7.seq:::::::::::::::
    CCGTCGANGCCGCCGACTTGGCTTGACCGACACCAACATGGCCTGAGGGTGTTCAACAAGACCGTGGCCGACGGGCTGAACATCACCATGAGCGGCATGAGCCACG
    CCACCGAGTTCATCATGTTGATCGCCGAAAACCATTGGCGGGTAGCGGAAGAACGGTCGAGGTGCTCTACACCCGAGTATTCGAAGTCGAAAGGCCAACCGTGCTC
    AACGGCGTCAACATCATTTTCGACGGGTTTCTGCGAGGGAGGATGCCACGATGAACTGGATCCAGGTGCTGTTGATCGCGTCGATCATCGGGTTGCTGTTCTACCT
    GTTGCGGTCGCGCCGAAGCGCGCGGTCCGTGCCTGGGTCAAGGTGGGCTATGTCTTGTTCGTGCTCCCGGCATCTATGCCGTGCTGAGA
    Clone Rv27
    :::::::::::::::Rv27SP6.seq:::::::::::::::
    TTACACGNCCTGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGAC
    ACTATAGAATACTCAAGCTTTTTGAGCGTCGCGCGGGGCAGCTTCGCCGGCAATTCTACTAGCGAGAAGTCTGGCCCGATNCGGATCTGACCGAAGTCGCTGCGGT
    GCAGCCCACCCTCATTGGCGATGGCGCCGACNATGGCGCCTGGACCGATCTTGTGCCGCTTGCCGACGGNGACGCGGTANGTGGTCAAGTCCGGTCTACNCTTGGG
    CCTTTGCGGACGGTCCCGACGCTGGTCGCGGTTGCGCGGCGGAAAGCGGCGGGTCGGGTGCCATCAGGAATGCCTCACCGCCGCGGCACTGNACGGCCAGTGCCGC
    GGCGATGTCNGCCATCGGGACATCATGCTCGCGTTCATACTCCTCGACC
    :::::::::::::::Rv27T.seq:::::::::::::::
    CAGGCATGCAAGCTTTGTCACACCAAGTGTTTCGACCAGGCGCTCCATCCGGCGAGTGGATACTCCCAGCAGGTAGCAGGTCGCCACCACGCTGGTCAGTGCGCGT
    TCAGCTCGCTTGCGGCGCTGCAGCAGCCAGTCCGGGAAATAGCTGCCCTGGCGCAGCTTGGGGATCGCGACGTCGATGGTTGCGGCACGGGTGTCGAAATCACGGT
    GGCGGTAGCCGTTGCGCTGATTGGACCGCTCATCGCTGCGTTCGCGGTAGCCCGCCCCGCACAGGGCGTCGGCTTCAGCCCCCATCAAGGCGG
    Clone Rv280
    :::::::::::::::Rv280SP6.seq:::::::::::::::
    AGCTTAGCCAGTTTTTCTACTCTTGGGCCCACACCCACAGTGCTTCGACGGTACGGTCACCCATGATGGCCATCCAGTTGGCATCGGTGAGCTGATAAATGCCAGC
    TGGTTTCGCCAACCCGGTAGCGATCTTGGCGCGCTGCTTGTTGTCACTGATACCTATCGAGCAAGACAGCCCGGTTTGCGACAAGATGACTTTTCGGATCTCTTCG
    GCGACTTCGATGGGGTCGTCGGGAGTCCCGGGCGCCACCGCGAGGTAAGCCTCGTCCCAGCCCCATACCTCGACCGGGTATCCCAGGTCGCGCAATAACGCCACCA
    CCTCCTCGGACGCCGCGTTGTAGGCGGCTGGGTTCGACGGCAAGAAGTGGCCTCAGGGCATCGTCGGCGCGGTCCCAACGGCNTGCCGGCGCGCACACCGTAGGCG
    CGGGGCTC
    :::::::::::::::Rv280T7.seq:::::::::::::::
    CCGGCGGAACTCAGACGTGCTGGTGGTGCGGCATGGCACCGCGGGCAGCAAAGCGCACTTCTCGGGGGACGACAGCAAGCGACCGCTAGACAAGAGGGGTCGTGCG
    CAGGCAGAAGCGTTGGTACCACAGCTGCTGGCGTTCGGCGCCACCGATGTTTATGCCGCCGACCGGGTGCGCTGCCACCAGACGATGGAGCCACTCGCCGCGGAAC
    TGAACGTGACCATACACAACGAGCCCACCCTGACCGAAGAGTCCTACGCCAACAACCCCAAACGCGGCCGACACCGAGTGCTGCAGATCGTCGAGCAAGTAGGCAC
    ACCCGTGATCTGCACGCAGGGCAAGGTCATTCCCGATCTGATCACGTGGTGGTGCGAGCGCGACCGTGTGCCCCCGACAGTCCCGCAATCGCAAAGGCAGCACGTT
    GGTGT
    Clone Rv281
    :::::::::::::::Rv281SP6.seq:::::::::::::::
    GTATGGTCAGCTGTCCATCCGGCGCTGTCGGCCGAGCTGCCAGATCTCGTCAGCCGTAACCGGTTGCGGGATCCACGCGTGCGGGTTGTCTAC
    :::::::::::::::Rv281T7.seq:::::::::::::::
    CCGACTTTCCGCGGGTACCCGCTCAACTTTGTGTCNACCTCAACGCCATTGCCGGCACCTACTACGTGCACTCCAACTACTTCATCCTGACGCCGGAACAAATTGA
    CGCAGCGGTTCCGCTGACCAATACGGTCGGTCCCACGATGACCCAGTACTACATCATTCGCACGGAGAACCTGCCGCTGCTAGAGCCACTGCGATCGGTGCCGATC
    GTGGGGACCCACTGGCGAACCTGGTTCAACCAAACTTGAAGGTGATTGTTAACCTGGGCTACGCGACCCGGCCTATGGTTATTCGACCTCGGCCGCCCAATGTTGC
    GACTCCGTTCGGTTGTTCCAGAANGTCAGCCCG
    Clone Rv282
    :::::::::::::::Rv282SP6.seq:::::::::::::::
    GCACCGATGTCGGCGAGCACTTCGTCAACTTCCAGGGGTGCCCGCACCAAGTATTTCGACGAGTATTTCCGTCGGGCCGCCGCCGCCGGTGCGCGGCAGGTGGTCA
    TCCTGGCGGCGGGGCTGGGACTCGCGCGCGTACCGGCTGCCTCGGC
    :::::::::::::::Rv282T7.seq:::::::::::::::
    TGCACCCAACTTACTGAGCATGCTAACGCTGGTCGTGCGGGTCTTGTTCCCGCGTGTCGGCAGGGCACACGCTCGGGGCGTAGCTGGGAGAGGCCCCGGTCAAGCC
    CGGAGAGCAGTGCTCAGTCCGCCAGCTTGACCGACTTTCGATGAGAACGCGCTTCTCGCCGTATTGAACTGGCGTGCTGACGGTCGCTGAGCAGCGCTCGCCGAGT
    GCGGCCGCTGATTCTTTCATCGAGCCAGGACGCGCATTCGTGTTCGGCCGC
    Clone Rv283
    :::::::::::::::Rv283SP6.seq:::::::::::::::
    AGCTTACGGCCGGTCGACGCGACGAGTGGTTCATGACACCACAAACCGTCAACGCCTACTACAACCCGGGGATGAACGAAATCGTCTTCCCGCAGCGATTTTACAG
    CCACCATTTTTCGATCCGCAGGCCGACGAGGCCGCCAACTACGGCGGGATCGGGGCGCGTGATCGGGCACGATGATCGGGCACGGTTTCGACGATAGGGCGCCAAA
    TACGANGGCGACGCAATCTGGTCNATTGGTGGATCGA
    :::::::::::::::Rv283T7.seq:::::::::::::::
    ATGCGTCACGTCACCACAATCGCGAGGACCCAATCATGCCGCCCAGGGCGGCCAACCCAATGGTGGCCGCGAAGCGGCAGCTCGATCGCAGCGCCGGAGGTGCCGG
    CCGCCAGTTGATTCACGAACAGGGTGAGGTCATAGGCGGGCAGGATAGTGACGAACGCAAGACCTATATCTGCCGTCGGAGTAAGAATCGAGTAGCCGGTCGACCA
    ACGGAAGCGAAAGTGTCCGCGATGTTGATGAGCGTCGCCGGTTGTGGCGGCGGTGGC
    Clone Rv284
    :::::::::::::::Rv284SP6.seq:::::::::::::::
    AGCTTCACCAGCGTGCCGATGCTGTTCGCNACACCTCCCTACTATGCGCAATTCGCCGACACGGGTGGCATCAACACGGGCGATAAGGTGGACATCGCTGGGGTGA
    ACGTCGGGCTGGTGCGCTCGCTGGCAATCCGCGGCAACCGCGTGTTGATCGGATTCTCGTTGCCCGGCAAGACAATCGGGATGCAAAGCCGGGCAGCAATTGGCAC
    CGACACCATTCTTGGCCGTAAGAACCTGGAAATCGAACCCCGCGGTTCGGAGCCGTTGAAACCCAACGGTTTCCTGCCGTTGGCGCAGAACACTACGCCATACCAA
    ATCTATGACGCGTTCGTC
    :::::::::::::::Rv284T7.seq:::::::::::::::
    CTGCCGCGGTGGCGGTCAGCGCCTGGCAAGTCACCGCACCGCCGTCCGGTTCATCGGCAGGCTCCCCCGAAAAGGGCCCTGGCAACAGAAGGTGATCAATGAGCTC
    CCGCAGACCTTCGCCGATCTGGGACCGACATACGTGAAGTTCGGCCAGATCATCGCGTCCAGCCCGGGAGCATTCGGTGAGTCGCTGTCGCGGGGAATTCCGCGGC
    CTGCTCGACCGGGTGCCGCCCGCAAAAACCGACGAGGTGCACAAGCTCTTCGTCGAGGAACTCGGCGACGAGCCGGCCCGGCTGTTCGCCTCCTTCGAGGAAGAAC
    CGTTCGCGTCTGCGTCCATCGCCCAAGTGCACTACGCGACCTGCGCAGCGGCGAAGAAGTGTGGTCAACATCCACGGCCGGGCATCCGCCGCCGCGTTT
    Clone Rv285
    :::::::::::::::Rv285SP6.seq:::::::::::::::
    GATCGTGCCGGCCCCCCGGCGGCAGTAGCAGATCAGCTCGTCGAAATCGCGGCAACCAGTCCAGTCGATTTCCATACGGGCGCCGTCAATCAACTCTGCGAAGATC
    GCGATCGGCACCGGAAACCGGCGAGCCGCGTCAGCCAGCGCAACCAGCACCGGGATCGGATGAATCATCAATATTATCAAGTGATTTCCTGATGGCATCGAGCTCG
    GTGATCTTGGTCTCGGGGGCCAGCTCGCCGTCGGCGACGTGCGTCGATCCGGCGGCCGAGCGCATAGCCGCAAATAGTGCCGCTCGCTTTTCGCGCGGCAAGAGTC
    GGATGCCGTAATATANGTTTCTGGCGGCCGTGCGCGTGATCNACTCGGTGATTCGATACGCCTGTTCATCTCGGTCATGCCGTCCTC
    :::::::::::::::Rv285T7.seq:::::::::::::::
    GGTGGCGCAATGACCGAAACCACCCCAGCCCCGCAAACCCCGGCGGCCCCGGCCGGGCCCGCACAATCGTTCGTGTTGGAGCGGCCCATCCAGACCGTTGGGCGCC
    GTAAGGAGGCCGTGGTACGAGTGCGGCTGGTGCCCGGCACCGGCAAGTTCGACCTCAACGGCCGCAGCTTGGAGGACTACTTCCCAAACAAGGTGCACCAGCAGTT
    GATCAAGGCACCCCTGGTCACCGTGGATCGGGTGGAAAGTTTCGACATCTTTGCCCACCTGGGCGGCGGCGGCCCGTCGGGTCATGGCCGGCGCGCTGCGCCTGGG
    TATCGCCCGGGCATTGATTCTNGTATCGCCGGATGACCGGCCCGCGCTGAATAANGCCGGCTTCTTGACCGTGATCCACGCGCCACCGAACGCAAA
    Clone Rv286
    :::::::::::::::Rv286SP6.seq:::::::::::::::
    CACAATAGATTACTCAAGCTTCGAACCAGCGGCCTTATCACGTATCCCCGCTGAGACCTTGACCCTTAGGGCCGAAGTGACTTCGCTGCTGCTATGCCGACACCCG
    ATTTCCAGACGCTGCTGTTACACGACGGCCGGGCCGGTGGCCACCATCACGCTCAACCGCCCGGAACAGCTCAACACCATCGTCCCGCCCATGCCCGACGAGATCG
    AGGCCGCTATCGGGTTGGCCGAGCGCGACCAGGACATCAAGGTCATCGTGCTGCGCGGTGCCGGCCGCGCCTTCTCCGGCGGTTACAACTTCGGCGGCGGGTTCCA
    ACATTGGGGGCAT
    :::::::::::::::Rv286T7.seq:::::::::::::::
    TCAGGACGCTTATGGTTGGCAGATGGTCGCCCTGGCGTCGAATACGCGCGAGCGCATGAGCTCACCGGTTCGGAACAACGTATCGAAGAACGTCGCACTGCTGGCA
    GATGGTATCTCCGATGTGGTTGTAATTTGTATCCCAACTCTAACTGTGCTATCGGATCAGCGTGAATATCGAGATATTGCGAATGCGATGACAGGCCGCCATTCGG
    TTTATTCGCTTACGCTTCCCGGGTTCGATTCGTCTGATGCACTGCCGCAAAACGCGGATATGATTGTTGAAACCGTATCTAACGCAATTATTGATGTGGTAGGCGG
    CAGCTGCCGTTTTGTGCTGTCGGGCTATTCATCGGGTGGGGGTGTTTGGCTATGCCCTCTGCTCCCAT
    Clone Rv287
    :::::::::::::::Rv287SP6.seq:::::::::::::::
    CGCAGCTGTCGCCGATCTGGTCCGGAATACCAGCTCCAGGTTCTGAGTGGAAGATGAGTGCGGGCCATCGAAGTGTTGTCAATGTACTCCAGGATGTCAGGTGCCG
    GCCGCTGGCGAGGATCTTGGGCACCGCCGCCATGACTTGGTCGAAGTCGGCGAACGGGGCGAGCACGCTGGCGTCGTGGTC
    :::::::::::::::Rv287T7.seq:::::::::::::::
    GTAGTTCGTTCATCCAAACACAGTGCGGTACCGGCTCAAGCGGATCACCGACTTCACCGGGCGCGATCCCACCCAGCCACGCGATGCCTATGTCCTTCGGGTGGCG
    GCCACCGTGGGTCAACTCAACTATCCGACGCCGCACTGAAGCATCGACAGCAATGCCGTGTCATAGATTCCCTCGCCGGTCAGAGGGGGTCCAGCAGGGGCCCCGG
    AAAAGATACCAGGGGCGCCGTCGGACCGA
    Clone Rv288
    :::::::::::::::Rv288SP6.seq:::::::::::::::
    TCCGCTCGCTTCTCCGAGAGGTTGAGTGCCAACGCTCTGCCGATGCCCGAAGCCGGCCCCGGTGATGACGGCGACCTTGCCTTCGAATGAGCTCATTTGACTACTC
    CCCGTGGTTGTCCCTGCGATTGGTGGAGGTGGCCGCGCAGCCTTGCCCCGAGGTCGGCGATCGCGTCTCGGGCTTCGGGGAGCAGACTGACCTGCAGATGGAAGTC
    GTGCCACATGCCCGCGAACCGGCGATGCTCGATGCTTGTTTTCGAAGCGGCGCAGGCGGTTTCGATCTTGTCCGCGTCAACACNGATCGGATCGTCGCCCGCGGTC
    TGCATGACGAATGGGCG
    :::::::::::::::Rv288T7.seq:::::::::::::::
    ATGGGAGGCCACCGATTACCATCTTGCACACACCGATTCCGGGCTATTGATGTCCACGTTCGGTCCGCGAACCGCGCTGTGGCTGCTGCTGGCCAAAGGCGGAGGC
    GATACCGAAGTCAGTGCCCAAGCTTGGGTTCCACGCTCGCGCAGCCACGCCGTCACCTTTCCACGAGACCTCACCTGCCGATCCGAAATGGAATCGGCCGTGACGG
    AATTGGCGCAGCGAACACTCAACGAGGTGGTGGCTTCGTCGCGAACCGTCACCCGAGTCGCGGTCACCGTGCGCACGGCGACGTTCTACACCCGCACCAAGATCCG
    AAAGCTGCAAGCTCCCAGCACCGATCCCGACGTCATCACCGCTGCCGCCCGGCACGTTCTTGAACCTATTCGAGCTGGAATCGGCCGTCCGGTTGCTGGGAATTGC
    NGTTAAGAACTGGGCCT
    Clone Rv289
    :::::::::::::::Rv289SP6.seq:::::::::::::::
    GCTTTGCGCGCTTCTCCGAGAGGTTGGAGTGCCAACGCTCTGCCGATGCCCGAGCCGGCCCCGGTGATGACGGCGACCTTGCCTTCGAATGAGCTCATTTGACTAC
    TCCCCGTGGTTGTCCCTGCGATTGGTGGAGGTGGCCGCGCAGCCTTGCCCCGAGGTCGGCGATCGCGTCGCGGGCTTCGGGGAGCAAACTGACCTGCAGATGGAAG
    TCGTGCCACATGCCCGCGAACCGGCGATGCTCGATGCTTGTTTTCGAAGCGGCGCAGGCGGTTCGATCTTGTCCGCGTCAACGCAGATCGGATCGTCGCCCGCGGG
    TCTGCATGAAGAAT
    :::::::::::::::Rv289T7.seq:::::::::::::::
    CTCACGCAGCCACGCCGTCACCTTTCCACGAAGACCTCACCTGCCGATCCGAAATGGAATCGGCCGTGACGGAAATTGGCGCAGCGAAACACTCAACGAGGTGGTG
    GCTTCGTCGCGAACCGTCACCCGAGTCGCGGTCACCGTGCGCACGGCGACGTTCTACACCCGCACCAACATCCGAAAGCTGCAAGCTCCCAGCACCGATCCCGACG
    TCATCACCGCTGCCGCCCGGCACGTTCTTGACCTATTCGAGCTGGATCGGCCCGTCCGGTTGCTGGGAGTGCGGTTAGAAACTGGCCTAGAAACCGGCGGGCACAC
    CGCACCTGGGCGGGGN
    Clone Rv28
    :::::::::::::::Rv28SP6.seq:::::::::::::::
    TGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACACTATAGAAT
    ACTCAAGCTTGATGCCGCCGAAACCGAGCGTGAGCACGCCGCCAGCCACCACNCGCGGGTCGGGCGCCGGGCCCGGGTCGCCANGCTGCTCCGCTCGGTGATGGCA
    CGCCACCGCGACACCACCCGGCTGCGCTACGTCGAGCCATACCGGGCGGAGCTACATCGGCTCGGCCGCCCAGTGTTCGGGCCCTCTTTCGAAGTCGAAGTCGATA
    CCGATTGCGCATCCGCNGCCGCA
    :::::::::::::::Rv28T7.seq:::::::::::::::
    CAGGCATGCAAGCTTCACGTCCGTACGGCTCGGGTACGCTTCGGTCGCAGTGTGCAGTGATAGATGACGACCGGGAACCTCGTCTGCATCTTCCATAGCCCGCCAC
    ACCTTCAGTTGCTCACCGGAATCCAACCGGTAGAAGGTCGGCGAGCGCTCGGCATTGGTCATCGGGATATGCCGCTCGGGACGGTCAGAACCCTCGGGTCCGGCCA
    GCACTCCGCAGGCTTCGTCGGGGTGGTCGCGACGCGCATGGGCCACC
    Clone Rv290
    :::::::::::::::Rv290SP6.seq:::::::::::::::
    GCTTGTCTATCGTCCCGGCCAGGTCCGGCCAGTCAAGGTCGAAGGCCAGTCCGGTCTCCTCTCCGACTACGGCCAAGAACTGGGCGACGGTGTCAGTGCAGACCAG
    CGGAAACTGGTGGCGCCCTAGGCGAGCGACCGCCTCACAAACGGCGGTGACCGCGTTCTGGTCGTGCACCATCGAGCCGTGCCCAGCCCGGCCGCGTGCCGTCAGC
    CGCATCCACTGGATGCCCTTCTCGGCGGTTTCAATCAGGTACAGGCGACGTTCGCCACCATCGTGCCGGGGCACGGTTAGCGAGAAACCGCCGACTTCACGATTGC
    CTCGGTGATGCCGTCGAAACAGATCGGGCCT
    :::::::::::::::Rv290T7.seq:::::::::::::::
    GCGCGCCATGTTGAGGTTGTCCGACGGTGACGACGGTGAACCACAACTGTTTGACCTGTCCGCACACACCGTGTGGATCGGCGAGCGGACCCGACAAATCGATGGC
    GCGCACATCGCGTTTGCCCAGGTGATTGCTAATCCGGTCGGGGTCAAGTTGGGCCCCAACATGACCCCGGAACTGGCCGTGGAGTACGTCGAGCGGCTCGACCCGC
    ACAATAAGCCGGGCCGGCTGACTTGGTGAGCAGGATGGGCAACCACAAGGTCCGCGATCTGTTGCCACCGATCGTGGAGAACGTCCATGCCACCGGGCATCAGGTC
    ATCGGC
    Clone Rv291
    :::::::::::::::Rv291SP6.seq:::::::::::::::
    TTGCCTTCCATGCCGAGCAAGGTCGACTCAGCGATGACGAATTGTTCTTCTTCGCGGGTGTTGCTGCTGGTTGCGGGCTATGAGAGCACTGCTCATATGATTAGCA
    CATTGTTTCTGACGCTGGCCGACTATCCAGATCAGCTGACACTCCTTGCGCAGCAACCAGACCTGATCCCGCCGGCGATCGAGGA
    :::::::::::::::Rv291T7.seq:::::::::::::::
    CGACGCTGGGCCCAACTGCGACCACCAGGTCCTGGTATGGCAGGACATGGCCGGGTTCAGCGGCGCCAATACCG
    Clone Rv292
    :::::::::::::::Rv292SP6.seq:::::::::::::::
    TAACGACTCGGGTCCAGCGACCGCGCCAACACNAACGGCCGGACNACGTGGGCCAGGGTCGCGGCCTCCCCTACAAACAGGATCCGTTGCCTGCGAACGACAGGCT
    CCGGTGCGGCGTTGGGCGCCGTGCTCGTCCCAGCGTCCGGTCCCGGGTCGCCGGCGACGCTTGTTTCCTCCATACTCGCCCCCTAATCTCGAGGCAGCCCGTACCC
    GCAGGCAACCTCCCAAAAATGCAATCCCCCAAAATGCAATGCGTCNAGCTATTTCTCACACCGACCGCTAGTTGCGGATCANAAATCCGTTGGGCGCGGA
    :::::::::::::::Rv292T7.seq:::::::::::::::
    CNTGGCGGTGGGTGCGGTGTCGAACACGACCACACTTCTTTGCGGTTCGGTGATCTCGACACCGGCCGCGAGCCGACCACCATGCGCGCGTAGATCGGCGATCAGC
    GCGTCGGCTATCGCCTGGGTGCCGCCCACCGGAATCGGCCAGCCGACCGAATGGGCCAGCGTTGCCATCATCAGTCCGGCGCCGGCCGACACCAGTGACGGCAACG
    GTGAAATCNCGTGGGCGGCAACGCCGGTGAACAACGCGCGGGCATCCTCGCCCGCCAGCGACCGCCAGGCAGGGGTGCCCTGGGCCAGCATCCGCAGCCCGAGACN
    CAGGACCGANCCCAGTG
    Clone Rv293
    :::::::::::::::Rv293SP6.seq:::::::::::::::
    GCTTTTCNGATCGCAGCGAGTCGTACCCGCGCCGGTCACCTTCGTGGATATCGCCGGCCTGGTCAAGGGGGCGTCCGAGGGAGCCGGGCTGGGTAACAAGTTCCTG
    GCTCATATCCGCGAATGCNACGCCATTTGTCAGGTGGTGCGGGTGTTCGTCAACAACNACTTGACTCATGTCACCGGACGGGTCGATCCCCANTCCGACATTGAGG
    TCGTCGANACCGAGCTGATCCTGGCANATCTGCAAACCCTGGAGCGGGCCACGGGCCGGCTGGAGAAGGAANCGCGCACCAACAAGGCGCGCAAGCCGGTCTACGA
    CGCGGCACTGCGTGCCCAGCAGGTGCTCGACGCCGGCAANACGCTGTTCGCCGCGGGGGTGGATGCCG
    :::::::::::::::Rv293T7.seq:::::::::::::::
    GTCGTACGCCATTNGTCGGTGTGCGCATACCAGTACGACGCGCCGGGCACCTGACGCGGCGGCCGCGACCAGTCGGTGGCCATCGCCATCGTCTGCCACCCGGTCA
    ACGGACGCACCTTCTCCTGGCCGACGTAGTGCGCCCACCCGCCGCCGTTGCGTCCCATCNATCCGGTCAACATGAGCAGCGCCAACACCGAGCGGTACATGACATC
    GCTGTGGAACCAGTGACAGATTCCGCCGCCCATGATGATCATCGACCGTCCTCCGGATTCGGTCGCGTTGCGGGCGAAATTCCTTGGCAAACCGGATTGCCTGCGC
    GGCCGGCACACCGGTGATCGACTCCTGCCAGGCCGGGGTGTTCTGCTGGGTTCGGTCGTGGTACCGGT
    Clone Rv294
    :::::::::::::::Rv294SP6.seq:::::::::::::::
    GCGAGGCGGTATCGCTTCCCGTCGTACCGGCGACCGCCAGCCGAGAAGCTCGTTTTCCCAGTGTTGCTGGGGATTCTCACGCTGCTGCTGANTGCGTGCCANACCG
    CTTCCGCTTCGGGTTACAACGAGCCGCGGGGCTACGATCGTGCGACGCTGAANTTGGTGTTCTCCATGGACTTGGGGATGTGCCTGAACCGGTTCACCTACNACTC
    CAAGCTGGCGCCGTCTCGTCCGCAGGTCGTTGCTTGCGATAGCCGGGAGGCCCGGATCCGCAATGACGGATTCCATGCCAACGCTCCGAGTTGCATGCGGATCGAA
    TACNAATTGATCACCCA
    :::::::::::::::Rv294T7.seq:::::::::::::::
    TGGGTCTTGCCGGCGAGCCCAGCGAAGTCGCTAGCGTGGCCGTGTTTCTTGGCTTCGGATCTATCCTCGTTACATGACCGGCACCGTGTTGGACGTGACTGGCGGC
    CGGTTCATATGACACCGAGATCATTGCCACGGTACGGCAATTCGTCAAGAAGGAAATCTTTCCCNATGCACCGGCCCTCGAACGTGGCAACAGCTACCCGCAAGAA
    ATCGTCGATCGGCTGGGTGTTATGGCTTGCTCGGTCGCCGGCTGCAAGGGTATCGACACCACCGAGTTCATTCTCGGGCGTGCCGGCCGCATTCGAGCTGCCGGTG
    CGCGCTGCCCAGCACCGTCATAAGTACTTGANGATGGTCAAACGTCGGACGAACCGCCACCACGTCGCTGCCGAACGG
    Clone Rv295
    :::::::::::::::Rv295SP6.seq:::::::::::::::
    TAGATGCCCAAGCTTGCCNTTANAGACCTCGTCGACCAAGCACGGACGCGACCGTCGAAGGTGGCGAATCCGGGCTTGGCGTCNACCCGCGTAAGGCAGACCAGAT
    GGTTCGCGGCACGGTCAACCTGCCACACGGCACTGGTAAGACTGCCCGCGTCGCGGTATTCGCGGTTGGTGAAAAGGCCGATGCTGCCGTTGCCGCGGGGGCGGAT
    GTTGTCGGGAGTGACGATCTGATCGAGAGGATTCAGGGCGGCTGGCTGGA
    :::::::::::::::Rv295T7.seq:::::::::::::::
    TCTCCACGGCGTGGATCAAGGTACCGGCCGGGATGTTGCGCAATGGCAGGTTGTTGCCCGGCTTGATGTCTGCGTTAGCGCCGGATTCCACCACATCCCCTTGCGA
    AAAGTCCGTTGGGTGCAATGATGTAGCGCTTCTCCCCATCGAGATAGTGGAGCAACGCAATCCGTGCGGTACGGTTCGGGTCGTACTCGATGTGCGCGACCTTGGC
    GTTGACACCATCTTTGTCATTGCGGCGAAAGTCGATCATCCGGTAAGCGCGCTTATGACCGCCGCCTTTGTGCCGGGTNGGTAATCCGGCC
    Clone Rv296
    :::::::::::::::Rv296SP6.seq:::::::::::::::
    GCCCGGTTCGATCGGGCATGTCCGCAGTCGTCGTTACCGGAGGCGGTCGTGGCCGCGCTAATCGGCGTCGGCGCCGACAAGATGTGGGATATCCGCAATCGGGGCG
    TCATCCCTGCGGGCGCGCTCCCCCGCGTCCGAGCCTTCGTCGACGCAATCGAGGCAAGTCACGACGCGGATGAGGGGCAGCAGTGAATTACAGCGAGGTCGAGCTG
    TTGAGTCGCGCTCATCAACTGTTCGCCGGAAACAGTCGGCGACCGGGGTTGGATGCGGGCACCACACCCTACGGGGGATCTGCTGTCTCGGGCTGCCGACCTGAAT
    GTNGGTGCGGGCANCGCCGGTATCNACTCCCGTGGAACACAGCCGGGGC
    :::::::::::::::Rv296T7.seq:::::::::::::::
    CTCGGCGTGGATATCGGTGTAGCCGGCGCCGGTGAANGTCGGCTCCTTACGTCCACTCGACAACAGCTCATAGCGATCCAACCAGTANGCAACCGCCTTCAGCAGT
    ACAACCGCGCCGGCGAACACTGCGAGTTGAACGCGAGCTGCCTGGGTCAGCATGCCTCTGCCGGTTGTCAGCCGAAGGCCGCCGAACAGGTAATGCGTCAACAGGC
    TCGCTAGAAACGCCAGAACCACGGCCACGAACAGCCAGTTCAGCACCGACCGGTAGAACGGCAGATCGAAGACGAAAAAACCCAATGTCATAGCCGAATTCGGGGT
    CCACGATGCCAAAGGTGCCCCCCGTGTACAACAACTGAACTTCACCCA
    Clone Rv29
    :::::::::::::::Rv29SP6.seq:::::::::::::::
    TCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACACTATAGAATACTC
    AAGCTTCACGTCCGTACGGCTCGGGTACGCTTCGGTCGCAGTGTGCGAGTGATAGATGACGACCGGGACCTCGTCGGCATCTTCCATAGCCCGCCACACCTTCAGT
    TGCTCACCGGAATCCAACCGGTAGAAGGTCGGCGAGCGCTCGGCATTGGTCATCGGGATATGCCGCTCGGGACGGTCAGAGCCCTCGGGTCCGGCCAGCACTCCGC
    AGGCTTCGTCGGGGTGGTCGCGACGCGCATGGGCCACCATCGCATTCACCAGGTCTGCGCGAATCACCAGCACGTAGACGGTTCCTTTCCTAAGCAACACCGAAGT
    TTCAGGACCGAATGCTCCGGGAAACATGTCA
    :::::::::::::::Rv29T7.seq:::::::::::::::
    CAGGCATGCAAGCTTGATGCCGCCGAAACCGAGCGTGAGCACGCCGCCAGCCACCACGCCCGGGTCGGGCGCCGGGCCCGGGCCGCCAGGCTGCTCCGCTCGGTGA
    TGGCACGCCACCGCGACACCACCCGGCTGCGCTACGTCGAGCCATACCGGGCGGAGCTCCATCCGCTCGGCCGCCAGTGTCCGGGCCCTC
    Clone Rv2
    :::::::::::::::Rv2SP6.seq:::::::::::::::
    CCTGCATCCGGCTCGTATGTTGTGTGGAATTGTGANCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACACTATAGA
    ATACTCAAGCTTCCAATCCCCCTGCCCTGATACGCGTCGGCAACCGTGAACGCGATCTCGGCGACCGTCGGATCGGTTTCATCCCGCACAAAACGCGCGTCGGCTA
    CGGGGTCGCTTCCGTCGGTCACCACCCAGACGAAGTGGTCGACGTAGTCGACTTCCGACAGGTAGTGCATCAACGCCGGACTGGGAACACNAGCCGACTGAACCCG
    TCGATACAGCGTCTCNCCGGAGAACTGGATGTGTCCGTGCACGGTCCGCTCGCGGTCACCGGGCAGCACGGGGCGTAACATCAGTTGAGTCCCGTCGGCAAGCCGT
    ACCGGAATCGGGGAGACGA
    :::::::::::::::Rv2T7.seq:::::::::::::::
    CAAGATGATCGCCGGTGCCACCCCGATCCGTGCCTCGGTCAGCGCGAACGTGCTTTCCGGTCCGGCGACCACCATGTCGCACGCACCGACCAGGCCGAACCCGCCG
    GCCCGCACATGCCCGTTGATGGCGCCGACCACCGGCAGCGGCGACTCGACGATGGCGCGCAACAGCGCCGTCATTTCCCGCGCCCGCGCCACCGCCATCCGGTACG
    GATCACCACCACCACCGCCGGCCTCGCTGAGGTCCGCGCCGGCGCAGAACGTTCCGCCGGTATGCCCCAGCACGACCAGCCGCACCGCCGGATCTGCTTCGGCCGC
    ACTCAGCCCTTGATGTAGTTGGCTGACCAGCGTGCTCGACAGCGCGTTGCGGTTGTGCGGAGAGTTCAGTGTCAGCCTGGCGAAGGGGCCGCCGCAGGCGGCCGGG
    CCAGCGTAGTCGACGGGGCTG
    Clone Rv301
    :::::::::::::::Rv301SP6.seq:::::::::::::::
    CTCAAGCTTCGATCGACAGTACTCCCGCCTTGGGTCTGGTCTTCGAGCTGGTCGGTCATGGTCGGACCTGCTGGTAGTGGGGATCTAACGCAACATGGTCGGGATT
    CATCATGGTGTACCCGTGATACCCATTCGCAGCTGCCGGTGAAACCCCGCGATGCCGGGATTTCCAGCCGACTAGGATGTCTAGCCGGCCAGCCGCTGCCGCCCGG
    ACTTCGGGATGTTCGGTATACCACCGATCGGCAATCTTGCNTATCCGCCGATGCTCGAACGCTAGCCACCCCAAACCAACCACTGTGACNACAATC
    :::::::::::::::Rv301T7.seq:::::::::::::::
    TGAATTTCCCGATCCCACAATCTCGGTTCAGATACAGGTCGCCATACCCCTTACTTCGGCAACGCTGGGCGGATTGGCCCTGCCGCTGCAGCAGACCATCGACGCC
    ATCGAATTGCCGGCAATCTCGTTCAGCCAATCCATACCCATCGACATTCCGCCGATCGACATCCCGGCCTCCACTATCAACGGAATTTCGATGTCGGAGGTCGTGC
    CGATCGATGTGTCCGTCGACATTCCGG
    Clone Rv302
    :::::::::::::::Rv302SP6.seq:::::::::::::::
    TACTCAAGCTTGAACGCTGCGAGCGAGCCCATGTAGAGCGTTTGGTACCAAACCGATCGGTGGGCCAACTTGCCATGGGCTCACAGCGGCTATCGCGAGCGTGTAG
    CCGATCATCGGCCAGGCGACGGTGGCCTGAGCGGCAGGGGTTGCCTTATCCATCCTCTTGCGGCATGGTTGCCGCAGGGAGTGCCGGTAAGTCTGGTCGGCAACCT
    GGCCCGCTGCGGGTTGGGTTCGGATTCCCTCGGCTAGTAAGGTGCTCGCCTGGTGTTACAACGAATCGCTAGACAGCTCTTATCGGGAGTGGCCGTCGCGATCGTT
    GCGCTGCCGCTGGCGATCGCGTTCGGCNTTACCGCCACCGGAACGTCCCAAGGTGCGCTCATCGGGCTCTACGGCGCCATCTTCGCCGGATTCTTCCCNGCCGTGT
    TCGGTGG
    :::::::::::::::Rv302T7.seq:::::::::::::::
    GCGGTGTCTGAACTTCGCCCGTTCCCTCCAGCGCATTGAGCTTCAGCCCGACCGGCAGGTAGGGAGTCGGCATGCGGTCCTTCGCCCCGAGCCCGCTGGCTAAATA
    GCCACCCCCGAGCGCGGTCACGGTCTTTGCACCGGGACGACGGCATACCGGCAGCGCGAACATCGCCGCGGGCTGCAGCGTGAACGTCGAATACGAGTCGAACAGT
    GTCGGCGCGTAAAAACCCGAGCCGGCGGTCGCTTCGGTAATCAACGGCTCCTGCGCAACCAGCTGCAANTCNCCGGTGCCACCGGCGTTGACAATCTTGATNTCGG
    CGACCTCGCGCACCAN
    Clone Rv303
    :::::::::::::::Rv303SP6.seq:::::::::::::::
    TACTCAGCTTCGGCTCAGGTGGTGCTGCTGGTAAAGTTCNCTGAACGGTGCAAGGTTTCGACAATGTGGTGCCGGTTCGGCGGGTACTGCCATCGAGACACTGGCG
    AGGCTATCGCACCCGTTATCGGCTACAAACAAATCGCGGTATGCGTTCTTGAGCATGAGTCGGCGACCGTCGTCATGGTCGACACCCACGACGGAAAGACGCAGAT
    CGCCGTCAAGCNTGTGTGCCGCGGATTATCAGGACTGACCTCCTGGCTGACCGGCNTGTTTGGTCNCGATGCCTGGCGCCCGGCCGGCGT
    :::::::::::::::Rv303T7.seq:::::::::::::::
    CATCACCTGGTTCATGAAACTGGAAGCAGCGCAGCGCTTCCTTTTCGGCCGCAACATGAGCCAGCCTCTCGTCGGCGGTCGGGTGCAGGTGCTCGGGCAGCTCGGC
    CGCGACAGCCGCCTGACCCTGAAACCAGCTTCCATATCCCGCGACGAACGACGCCAGTCCGCTACGTAACCCCTCCGCGACTGTCCATGGACAACANCGCGTTCTC
    CACCGACCGGGCCCGGGTGTGGGGTGTT
    Clone Rv304
    :::::::::::::::Rv304SP6.seq:::::::::::::::
    CTCAAGCTTCCCGGCGGCCAGTACCGAAAGCGCGAACAGCTCGCGGCAGCCCACAACNTGCTGCGTCGGATTGCCGGCGGCGANATCAATTCCAGGCAGCTCCCGG
    ACAATGCGGCTCTGCTGGCCCGCAACGAAGGACTCGAGGTCACCCCGGTGCCCGGGGTCGTGGTGCACCTGCCGATCGCACAGGTTGGCCCACAACCGGCCGCTTG
    ATGCCCGGTCGGCAAGCCCCGGCAGTTGCCAAACCCAGCGTGATCAGGCTCGGCTCGCGAGTTCGGCGAAGAAGTGGCTCGCCTGATCACCTACCATCGGCCAGGT
    CTGCGTGTCATCACNACGCTCGCCAAGGAAGGTTGTTGTGGTGCT
    :::::::::::::::Rv304T7.seq:::::::::::::::
    GCCACGTTTCGCGCCGCCCGGCATACGGCGGCGTACCGATCTCCGCGTCATACACCCGCGGGTAATCGCCGACGGTGCCGGTTCGCGAGCCGAAGGTGACGACGCT
    GATTGAATCGAGTTCCAGGTCCAGCGGGTGGCGCAGCAACGGCGCGAGCTCAACGACGTCAATCACGTTGTCGCTTTCTACGGTCACCGACCCGGTGACCGTAGTC
    GCCCGGTGCGCTCGGCCGAGAAGTTGCACCGCCACCACCGCGACACCGTCTTGCACGCGGACGCCACCCCCCGGATCGGTTGTTGGCCAAGGTAATTGGGTCATCC
    ATTTGACGGGACGCCGACCCCGCAGCCCCAGTACCGCCCACGACCACGCCGGCTGACCCCACCACTGTACGAACACCAAGGCGACGCCGACCA
    Clone Rv306
    :::::::::::::::Rv306SP6.seq:::::::::::::::
    CTCAAGCTTGATGCCGCCTAAACCGAAGCGTGAGCACGCCGCCACCCACCACGCGCGGGTCGGGCGCGGGGCCCGGCGCCGCAGGCTGCTCCGCTCGGTGATGGCA
    CGCCACCGCGACACCACCCGGCTGCGCTACGTCAAGCCATACCGGGCGGAGCTACATCGGCTCGGCCGCCCAGTGTTCGGGCCCTCTTTCGAGGTCNAGGTCNATA
    CCGATTTGCGCATCCGCAGCCGCACCCTGGACGACAGAACCGTGCCCTACGAGTGCTTGTCGGGCGGGGCCAAAGAACANCTTGGCATCCTGGCGCGATTGGCCGG
    CGCGGTCCTGGTC
    :::::::::::::::Rv306T7.seq:::::::::::::::
    CTCGGGTACGCTTCGGTCGCAGTGTGCGAGTGATAGATGACGACCGGGACCTCGTCGGCATCTTCCATAGCCCGCCACACCTTCAGTTGCTCACCGGAATCCAACC
    GGTANAANGTCGGCGAGCGCTCGGCATTGGTCATCGGGATATGCCGCTCGGGACGGTCAGAGCCCTCGGGTCCGGCCAGCACTCCGCAGGCTTCGTCGGGGTGGTC
    GCGACNCGCATGGGCCACCATCGCATTCACCAGGTCTGCGCG
    Clone Rv307
    :::::::::::::::Rv307SP6.seq:::::::::::::::
    CTCAAGCTTCAATTCCTCCACGACGCGTTCCCAAATGAATTTCCCGATCCCACAATCTCGGTTCAGATACAGGTCGCCATACCCCTTACTTCGGCAACGCTGGGCG
    GATTGGCCCTGCCGCTGCAGCAAACCATCGACGCCATCGAATTGCCGGCAATCTCGTTCAGCCAATCCATACCCATCGACATTCCGCCGATCGACATCCCGGCCTC
    CACTATCAACGGAATTTCGATGTCGGAGGTCGTGCCGATCGATNTNTCCGTCNACATTCCGGNGGTCACCATCACCGGCACCAGNATCGACCCGATTCCGCTGAAC
    TTCGACGTTCTCAGCAGCGCCGGAACCA
    :::::::::::::::Rv307T7.seq:::::::::::::::
    TTAACCCCCGTGGCCTCTACGCCGCCTNCGGGTCGAACATGCATCCCGAGCANATGCTCGAGCGCGCACCCCACTCGCCGATGGCCGGAACCGGCTGGTTACCCGG
    GTGGCGGCTGACGTTCGGCGGCGAGGACATCGGCTGGGAAGGGGCGCTTGCCACCGTCGTCGAAGACCCAGATTCGAAGGTGTTCGTCGTGCTCTACGACATGACC
    CCGGCGGACGAGAAGAACCTTGACCGGTGGGAAGGCTCCGAGTTCGGCATCCACCANAAGATCCGATGCCGCGTT
    Clone Rv308
    :::::::::::::::Rv308SP6.seq:::::::::::::::
    CTCAAGCTTGATTTTGATCATCATGGATGATCATCACCCGAAGTGTGGTAGCCGCAGTGGTTATCGTGGGTACCGTCGTGCTTTCCATGGGCGCCTCTTTCGGGCT
    TTCCGTATTGGTCTGGCAGGACATTCTGGGTATCGAGTTGTACTGGATGGTGTTGGCGATGTCGGTGATCCTGCTCCTGGCGGTGGGATCCGACTACAATCTGCTG
    CTGATTTCCCGGTTGAAAAANGAAATTGGGGCCGGATTGAACACCGGAATTATCCGTGCCATGGCTGGTACCGGGGGAGTGGTGACGGCTGCCGGCATGGTGTTCG
    CCGTTACCATGTCGTTGTTTGTGTTCAGCGATTTGCGAATTATTGGTCAGAT
    :::::::::::::::Rv308T7.seq:::::::::::::::
    CGNCCAACCCGAATTGGTTTTCGGCGCCNTCGGTGAGGACGGCGTGCGGGTGCTCAACGACGACGTCGTCCGCGGGACACACCTCGATGCTGCCGCCATGGACGCG
    GTCGAACGCAAGCAGCTGATCGAGCTACAACGCCGCGCGGAACGCTTCCGCCNCNGGCGTTACCGCATCCCGTTGACCGGGCGGATCGCGGTGATCGTCGATGACG
    GCATCGCCACCGGAGCGACGGCCAAGGCGGCGTGCCAGGTCGCCCGGGCGCACGGTGCGGACAAGGTGGTGCTGGCGGTCCCGATCGGCCCANACGACATCGTGGC
    GAGATTCGCCGG
    Clone Rv309
    :::::::::::::::Rv309SP6.seq:::::::::::::::
    CGTGACTGCCACCGGGGCCACTCCGCAGAATCTGTACCCGACCAAGATCTACACCATCGAATACGACGGCGTCGCCGACTTTCCGCGGTACCCGCTCAACTTTGTG
    TCNACCCTCAACGCCATTGCCGGCACCTACTACGTGCACTCCAACTACTTCATCCTGACGCCGGAACAAATTGACNCAGCGGTTCCNCTGACCAATACGGTCGGTC
    CCACGATGACCCANTACTACNTCATTCGCACGGANAACCTGCCGCTGCTAGAGCCACTGCGATCGGTGCCGATCGTGGGGAACCCACTGGCGAACCTGGTTCAACC
    AAACTTGAAGGTGATTGTTAACCTGGGG
    :::::::::::::::Rv309T7.seq:::::::::::::::
    TCGCTCAAGCGCNTGAGGCCGAANCGGCTGGTTACGACTCCCTGTTTCTGATGGACCACTTCTACCAACTGCCCATGTTGGGGACGCCCGACCAGCCGATGCTGGA
    GGCCTACACGGGCCCTTGGTGCGCTGGCCACGCGACCGAGCGGCTGCAACTGGGCGCGTTGGTGACCGGCAATACCTACCGCAGCCCGACCCTGCTGGCAAAGATC
    ATCACCACGCTCGACGTGGTTAGCGCCGGTCGAGCGATCCTCGGCATTGGAGCCGGTTGGTTTGAGCTGGAACACCGCCAGCTCGGCTTCGAGTTCGGCACTTTCA
    GTGACCGGTTCAACCGGCTCGAAAAGGCGCTACANAT
    Clone Rv30
    :::::::::::::::Rv30SP6.seq:::::::::::::::
    ATACTCAAGCTTCCGCTGGGGCCTGTTCAACCATGGCGATCCCGTTGGTCCCGGACATCCCGAACGAGGACACCGCGACCCNCTTCGGTGTGTGATCATTACCGTT
    GGGCCACTGCGTAACCGCTTGCGGCACAAAGAGCCCGGTCTCGACGTCGGAAAAGCTCATCGGGCACCCGATTGAAATGCAGCAGCGGCGGCACCACCCGTGCCGC
    AGTGACAGAATTGCCTTGATCAGCCCGACGGTCCCCGCCGATGCCGTGCTGTGCCCCATGTTGCTCTTGGCCCGATCCAAGCGCGCAGGGGGTGCCGCGCCATACA
    CCCGCGCCAGGCTGCGGTACTCAATCGGGTCGCCGATTGGCGTACCGGTGCCGTGCGCCTCCACCACACCGACCGTTTCGGGCTG
    :::::::::::::::Rv30T7PEG.seq:::::::::::::::
    CAACAGCGTTCCAGCGGCATACCACCGCACATGCCGTGCACCCGGCGCCGGGCGGAGTCGCCGCATAACACANGTACACCTTGGGAATCGGTGTGCGCCAGGGATT
    CNACCGCGGGGTGGGGCCGGCGATCGCGCGCCAGGTCGAGTTGGCGCCGACCGTGATNTCACCGCGACGTAGTTGGCGTTGTGGTCCGCCATCCGCGCGGCGGGGC
    ACGGCGCGGGCCGCCACCACGATGTCACGGAAGCCGGGGGCGAACGCTCGACGACCTGGTTACCGTCTCNGTCGCNTCNANCGTGGACCCGACNGCACGTGGGCAT
    ATGTCCANAACGGACGNGGCCGGTTTCNTCGATGCNGCCGGGGTCCGCGACNTGCGGACNCNCNGNCACACCATCCGCCAGTCCGCGTGGCGTCCCGCCGCGACTC
    TGCCTCGGCCGCGCCA
    Clone Rv310
    :::::::::::::::Rv310SP6.seq:::::::::::::::
    CTCAAGCTTTGNCGACGATCGGGCGATGTCGATGANAGGAAACCCCAGCGCACAACCGACNATTTTGGCGTAGCCGGCGGACNTCTGCTCGATTCCGATCACGTCG
    GCGCTCGCATCGAGCATGGCGCCGGCGACGGCTAGCAGCGATCCGCCGTCGTCGAGGAACACGACACGAGCCGTACGCCCGGCCGTAAGCCGCGCCCAGGATTCGG
    CGAAAAACCGTTCTACGTGGCGGGTGTACTGGGTGTCCAATGATTCGTGGGGTGCGTAGGCGTCGCTGCAATCGTCGACATAAATGCCGTCGGCCCGCATCGCGTC
    AACAACTCCCGGGTGAGTGGAATANCACTTGCCGA
    :::::::::::::::Rv310T7.seq:::::::::::::::
    TCCAACGCGGTGACAGATTTGTCTATCCTGGACCTGACGGTGAGGTCGAAGTTTTCCAGGAATTCGGCAAAATCGGTAAGAGCCTGAAGAATTCGGTATCGCCGGA
    CGAAATCTGCGACGCATACGGGGCAGATACGCTTCGGGTTTACGAGATGTCGATGGGGCCGCTGGAGGCTTCACGTCCATGGGCCACAAAGGATGTTGTCGGCGCG
    TACCGTTTTCTGCAGCGGGTGTGGCGCTTGGTCGTCGACGAGCACACCGGCGAAACTCGGGTGGCTGACGGCGTGGAACTCGACATCGATACGCTACGGGCGTTGC
    ACCGCACCATCGTCGGCGTGTC
    Clone Rv311
    :::::::::::::::Rv311SP6.seq:::::::::::::::
    CTCGTCCTTGACTACGCCCAGTATCGAAANCCTCCTGTGCCGGTNCGCTAAACACCCGGCGGACACTCANACGGTGCTGGTGGTGCGGCATGGCACCGCGGGCAGC
    AAAGCGCACTTCTCCGGGGACGACAGCAAGCGACCGCTAGACAAGAGGGGTCGTGCGCAGGCAGAAGCGTTGGTACCACAGCTGCTGGCGTTCGGCGCCACCGATG
    TTTATGCCGCCGACCGGGTGCGCTGCCACCABACBATGGAGCCACTCGCCGCGGAACTGAACGTGACCATACACAACGAGCCCBCCCTGACCGAAGAGTCCTACGC
    CAACAACCCCAAACGCGGCCGACACCGAGTGCTGCAGATCTTCG
    :::::::::::::::Rv311T7.seq:::::::::::::::
    GTATCGCCTCCNCCTTTGGCCACCAGCAGCCACAGCGCGGTTCGCGGACCGAACGTGGACATCAATAGCCCGGAATCGGTGTGTGCAAGTTGGTAAACGGTGTTGA
    TCCCAAGCTTTGCCAGCCTTTTCGTAGTCTTGGGCCCCACACCCCACAGTGCTTCGACGGTACGGTCACCCATGATGGCCATCCAGTTGGCATCGGTGAGCTGATA
    GATGCCAGCTGGTTTCGCCAACCCGGTAGCGATCTTGGCGCGCTGCTTGTTGTCACTGATACCTATCGAGCAAGACAGCCCGGTTTGCGACAAGATGACTTTTCGG
    ATCTCTTCNGCGAACTTCCAATGGGGGTCTCCGGANT
    Clone Rv312
    :::::::::::::::Rv312SP6.seq:::::::::::::::
    CTCAAGCTTTTGGTCTAGCCGGCCGAGCACGATACGGGTGTCCTTGGCCACCGGCGGCGGCTGTCCGGGAAATGGCGGGTCCCCGGTGGTTTTGCTGANGANTGCT
    GAACCGTAGTCGAAGTGGGCGGCGTCAGACTCCACCCAGCCAGCAGGCAGCGCGAAGCTGAATCCTCCAACCGGGTTGTCGATCCGGACAGGTTGGGTGCGTTTTG
    GGGCAATGACAGGTGGCGGCGGTGCGTTCGGGTCGGCCGGCGGAGGTGCTGCGTTGGGATCNCCCGGCTGGGCATTCGGCNTTNTTGGCGGCGGCCGGTGGTGGGG
    GGCAACANGTGTCCCGGTGCGGGTGGCGCTGC
    :::::::::::::::Rv312T7.seq:::::::::::::::
    ATCTGTACCCGACCAAGATCTACACCATCGAATACGACGGCGTCGCCGACTTTCCCGCGGTACCCGCTCAACTTTCTCTCGACCCTCAACGCCATTGCGGCACCTA
    CTACGTGCACTCCAACTACTTCATCCTGACGCCGGAACAAATTGACGCAGCGGTTCCGCTGACCAATACGGTCGGTCCCACGATGACCCAGTACTACATCATTCGC
    ACGGAGAACCTGCCGCTGCTAGAGCCACTGCGATCGGTGCCGATCGTGGGGAACCCACTGGCGAACCTGGTTCAACCAAACTTGAAGGTGATTGTTAACCTGGGCT
    ACGGCGACCCGGCCTATGGTTATTCGACCTCGCCGCC
    Clone Rv313
    :::::::::::::::Rv313SP6.seq:::::::::::::::
    CTCAAGCTTGCAATGCGGGTCGGGATGCCCATGGTTGGAANATGGTCGCCCTGGCGTCNAATACGCGCGAGCGCATGAGCTCACCGGTTCGGAACAACGTATCGAA
    AAACGTCGCACTGCTGGCAGATGGTATCTCCGATGTGGTTGTAATTTGTATCCCAACTCTAACTGTGCTATCGGATCAGCGTGAATATCGANATATTGCGAATGCG
    ATGACAGGCCGCCATTCGGTTTATTCGCTTACGCTTCCCGGGTTCGATTCGTCTGATGCACTGCCGCAAAACGCGGATATGATTGTTGAAACCGTATCTAACGCAA
    TTATTGATGTGGTAGGCGGCAGCTGCCGTTTTGTGCTGTCGG
    :::::::::::::::Rv313T7.seq:::::::::::::::
    CAAATACACGCCGGACGCACAGGCGGACATCGCCATCCCGAGCACACCCAAAACGGGATACAGGATGGAGGCCAACGCCACGGCCGCGCCCAGGATCACCAACCAC
    ACCGGCTTGGTCAGCTTGTCGGCGGCGGTATAGGCATCGGGCCGCTGCAACGCAGCATGCACAAACGCGTACACCGCTGTCACCAAGACGGCGAGCAGCAATACCA
    GCATGACGGTACCCACGAGGTGGCTCACGCATTCAGACTATGCGGTTTGCATCCAACACG
    Clone Rv314
    :::::::::::::::Rv314SP6.seq:::::::::::::::
    CTCGTCCTTCGGCCTCGCTGCAGGAGTGGGAGCCGCAGGGCTGGAAATCCGAAAAACGAGCCGGTGATCGCACTGTCGCCGATCGGCGCCGCACCTGGTTGGTGTT
    ACGGATGAATCCGCAGCGAAATGTGGCTGCGGTGGCGTGTCGTGACTCGTTGGCGTCGACGCTGGTGGCAGCCACCGAGCGGTTGGTCCAGGATCTGGATGGGCAA
    AGTTGTGCGGCCCGGCCGGTGACGGCCGATGAGCTGACCGAGGTCGACAGCGCCGTGTTGGCTGACTTGGAACCGACATGGAGTCGCCCCGGTT
    :::::::::::::::Rv314T7.seq:::::::::::::::
    GTCTAGNCCGCCGAACACGATACGGGTGTCATTGGCCACCGGCGGCGGCTGTCCGGGAAATGCGCGGGTCCCCGGTGGTTTGCTGAAGANTGCTGAACCGTAGTCG
    AAGTGGGCGGCGTCAGACTCCACCCAGCCAGCAGGCAGCGCGAAGCTGAATCCTCCAACCGGGTTGTCGATCCGGACAGGTTGGGGTGCGTTTGGGGCAATGACAG
    GTGGCGGCGGTGCGTTCGGGTCGGCCGGCGGAAGTGCTGCGTTGGATCGCCCGGCTGGGCATTCGGCGTGTTGGCGGCGGCCGGTGG
    Clone Rv315
    :::::::::::::::Rv315SP6.seq:::::::::::::::
    ATCAAGCTTGAGATTGGGCGTCAACGGGTGTCGGCACCGGCGTCCTGCAGTTGGTAGGCCTGCAGTTTGTGCATCAGGCCGATGCCGCGGCCCCTCGTGCCACGCA
    TGTACANCACCACGCCGCGCCCCTCACGGGCGACCATCGCCAGCGCGGCGTCCAGCTGAGGCCCCGCAATCGCAGCGGCGTGACCAAACACATCGCCGGTCAAGCA
    CTCCGAATGCACCCGGACCAGCACGTCG
    TCACCGTCGGCGTTGGGCCCGGCGATCTCGCCGCGGACCAGCGCGACATGTTCCACGTCCTGTAAATGCTGGTGTANCCGATGGCGCGAAACTCCCCCATGACAAN
    TCGGAATCCCGCGCCTCGGCGACCCCGCTCAATGTTGCTTCTCNTGCTTG
    :::::::::::::::Rv315T7.seq:::::::::::::::
    TCGACNAGCATTCTTGACNGTTGTTTTGGCTCGGCATGGTTAGCCAAGGTTCTGCGGTCCCACCAGATCATCTTGGTCCGGTAGCGCTCGTCCGGGTATGCTGCCG
    CCGGGATTCTCGCTGCTATTACTCCCCCCGAAGAACGCCACCGGTCCAGCGCGTGGCCGCCGCGGTCCCCATCACAAACTGAACCCCCAACAGGGGGACATGCTTA
    GCGGTAGGGCGCGCGCCAAGGCGGCAGCAATCGCATCACTGCGCTGCGCGTCACTATTAACCCAGCCGGACTTCACTTCCACGACCCCCGAATGGCGCCGGTCATT
    GATCATCTTGCGCACCCGCGGATAATCCGGGAT
    TG
    Clone Rv316
    :::::::::::::::Rv316SP6.seq:::::::::::::::
    ACCGGGGCCACTCCGCACAATCTGTACCCGACCAANATCTACACCATCGAATACGACGGCGTCGCCGACTTTCCGGCGGTACCCGCTCAACTTTGTTCNACCCTCA
    ACGCCATTGCCGGCACCTACTACGTGCACTCCAACTACTTCATCCTGACGCCGGAACAAATTGACGCNGCGGTTCCGCTGACCAATACGGTCGGTCCCACNATGAC
    CCANTACTACATCATTCGCACGGANAACCTGCCGCTGCTAAAGCCACTGCGATCGGTGCCGATCGTGGGAACCCACTGGCGAACCTGGTTCAACCCAAACTTGAAG
    GTNATTGTTNACCTGGGCTACGGCGANCCGGCCTNTGGTTATTCCACCTCNCCGCCCAATGTTTGCNACTCCCGTTCGGGGTTGTTCCCNAAGGTCAAACCC
    :::::::::::::::Rv316T7.seq:::::::::::::::
    CGCTCAAGCGCNTGAGGCCGAANCGGCTGGTTACGACTCCCTGTTTGTGATGGACCACTTCTACCAACTGCCCATGTTGGGGACGCCCGACCAGCCGATGCTGGAG
    GCCTACACGGCCCTTGGTGCGCTGGCCACGGCGACCGAGCGGCTGCAACTGGGCGCGTTGGTGACCGGCAATACCTACCGCAGCCCGACCCTGCTGGCAAAGATCA
    TCACCACGCTCGACGTGGTTAGCGCCGGTCGAGCGATCCTCGGCATTGGAGCGGTTGGTTTGANCTGGAACACCGCCAGCTCGGCTTCGAGTTCCGGCACTTTCAG
    TGACCGGTTC
    Clone Rv317
    :::::::::::::::Rv317SP6.seq:::::::::::::::
    CTCAAGCTTGCGTTCGATGAAGTAGTCGTCGGTCAGCGCCGCCTCTTCGAGCTCCTTGGCGATGCCCAGCAAGGAGTCATCGCCGCCGAGCTTGGCCAGGATCTTG
    TCGGCCTGTTCCTTGACGATGCGGGCCCGCGGATCGTAGTTCTTGTAGACACGATGACCGAAACCCATCAATTTGACCCCGGCCTCGCGGTTCTTGACCTTGCGTA
    CAAACTCGCTGACGTCGTCGCCGCTGTCGCGAATGCCCTCGAGCATCTCCAGGACAGCCTGATTGGCGCCGCCATGAAGCCGGACCCCATAGTGCGTTGATGCC
    :::::::::::::::Rv317T7.seq:::::::::::::::
    GGTCAGGCCCGAGCAGGCGCGAGGAACGACGAACCCAACAAGCCATGGTGGTTGGCGCCGTCGAGAGGTCGGCGGTCGCACAACGGGAAGATCGCCTTGAGCGTCG
    CTCGACCGCCGCCTCGAGTTGGGTCATAACGAAGTAGCTGATGCCGATCATGTCGACGTTTCCGTCGCATCAGCGTGCAGCGGCGACCCACTCNACCGAGGTCTCG
    TGCCGCCGCGGCCAGGGCACCAGCAGTGACGAGTCCAGGCCGCCGTCGGGCCAAGCAGTCGCGGTGCCANCCGTGGTGGGTCCGGCGATGGTTGGTGTGCTCATTT
    CGGGAACGCCA
    Clone Rv318
    :::::::::::::::Rv318SP6.seq:::::::::::::::
    CTCGAAGCTTTAACAGCATCAACCCCGCCCCGCACCAGCACCGACACNATGTCGATGCCATCGAAGGTGAATGTCGAACTGGCGCAAACCATCGCGACCGCGACCA
    CCGGCAACATGGGTACCGGCGATTTCCGGTGCCAATGCCGACCCGACGGGCCGCTCTCACCGCAGGTGACCTCGATCACCGAGACCANCCGGCCGTTNTNNTCACG
    CACCCCTACCGTGTCACGCCCAAAACGGCGCTGGTGGTCGATTGCCGGAGTGCACCCCNCACCCCAGTGTGCGTCCCCGGATCC
    :::::::::::::::Rv318T7.seq:::::::::::::::
    TGATGCCGCACCCGATCGACGGTCGTTGGTCGGGGTTGACTGGCGCCCGGCGAAGCAGGGCGTCGACCGCGGCCCGGACGTCGGCGGCCGTCACCGGGTCGGCCAT
    TGCCCGGGCGGGAGTCGTCGAGCTGACCACGGTAGACAAGTCGGCGCTGGCCGTCGAAGACNAACGTGCGGGTGTGCAGGCCGCGGAGAAGGCGCCGGCGACNTTC
    TTGGGTTTCGTCGTANAGATACGGGAACGTCCAGCCGTGGCGGCGGGCCTCGGCGACCATCTGATCGGCCCGTCC
    Clone Rv319
    :::::::::::::::Rv319SP6.seq:::::::::::::::
    TTTCGGGCGAGGCGGTATANCTTCCCNTCGTACCGGCGACCGCCAGCCGANAAGCTCGTTTTCCCAGTGTTGCTGGGGATTCTCACGCTGCTGCTGANTGCGTGCC
    AAACCGCTTCCGCTTCGGGTTACAACGAGCCGCGGGGCTACNATCGTGCGACGCTGAAGTTGGTGTTCTCCATGGACTTGGGGATGTGCCTGAACCGGTTCACCTA
    CNACTCCAAGCTGGCGCCGTCTCGTCCGCAGGTCGTTGCTTGCGATAGCCGGGAGGCCCGGATCCGCAATGACGGATTCCNTGCCANCGCTCCGAGTTGCNTGCGG
    ATCGACTACNAATTGATCACCCANAACCATCGGGCGTNTTACTGCCTGAAGTACCTGGTGCGGGTCGGATACTGCTATCCGGCGGTGACAACCCCGGCAAGC
    :::::::::::::::Rv319T7.seq:::::::::::::::
    GTTTTGGCTCGGCATGGTTAGCCAAGGTTCTGCGGTCCCACCAGTCATCTTGGTCCGGTAGCGCTCGTCCGGGATATGCTGCCGCCGGGATTCTCGCTGCTATTAC
    TCCCCCCCGAAGAACGCCACCGGTCCAGCGCGTGGGCCGCCGCGGTCCCCATCACAAACTGAACCCCCCAACAGGACATGCTTAGCGGTAGGCGCGCGCCAAGGCG
    GCAGCAATCGCATCACTGCGCTGCGCGTCACTATTAACCCACCCGGACTTCACTTCCACGACCCCGAATGGCGCCCGGTCATTGATCATCTTGCGCACCGCGGATA
    ATCCGGGATTGCCAGCCCATTCNACTACCGCATGCGAGTCATCGGCTGACCGCAGCGGTC
    Clone Rv31
    :::::::::::::::Rv31SP6.seq:::::::::::::::
    TCGCCTAGGCGGGCTTCCCCTTCCGTCCGAGCNGTCAGAAGCTCCTATGACAATGCACTACCCGAGACNATCAACGGCCTATGCAATACCNAGCTGATCAAACCCG
    GCAAGCCCTGGCGGTCCATCGAGGATGTCGAGTTGGCCACCGCGCGCTGGGTCGACTGGTTCAACCATCGCCGCCTCTACCGGTACTGCGGCGACATCCCGCCGGT
    CTAACTCGACGCCGCCTCACTACGCTCAACGCCAGAGACCANCCGCCGGCTGACGTCTCAGATCAGAGAGTCTCCGGACTCACCGGGGCGGTTCATCCCCACTGTC
    GATAGCGTCTGTGGATAACTTTGTCTGCA
    :::::::::::::::Rv31T7.seq:::::::::::::::
    GCGCGTNGAACTGATAGGTGCGGCCCGGCTCGAGCANGCCGGCCATTTGTTCGATGCGGTTACCGAAGATCTCTTCGGTGACCTGCCGCCCGCCGGCCAGCTCGGC
    CCAGTGCCCGGCGTTGGCCGCCGCGGCGACAATCTTGGCGTCCACGGTGGTCTGGGTCA
    Clone Rv321
    :::::::::::::::Rv321SP6.seq:::::::::::::::
    CTCAAGCTTCAATACAGAGTTATAAACTGTGATAATCAACCCTCATCAATGATGACNAACTAACCCCCGATATCAGGTCACATGACGAAGGGAAAGAGAAGGAAAT
    CAACTGTGACAAACTGCCCTCAAATTTGGCTTCCTTAAAAATTACAGTTCAAAAAGTATGAGAAAATCCATGCAGGCTGAAGGAAACAGCAATAACTGTGACAAAT
    TACCCTCAGTAGGTCAGAACAAATGTGACGAACCACCCTCAAATCTGTGACAGATACCCTCAGCTATCCTGTCGTCATGGAAGTGATATCGCCGGAAAGGAAAAT
    Clone RV322
    :::::::::::::::Rv322SP6.seq:::::::::::::::
    CTCAAGCTTCGATCGACATTACTCCCGCCTTGGGTCTGGTCTCCGAGCTGGTCGGTCATGGTCGGACCTGCTGGTAGTGGGGATCTAACGCAACATGGTCGGGATT
    CATCATGGTGTACCCGTGATACCCATTCGCAGCTGCCGGTGAACCCCCGCGATGCCGGGATTTCCAGCCGCACTAGGATGTCTAGCCGGCCAGCCGCTGCCGCCGG
    ACTTCGGGATGTTCGGTATACCANCGATCGGCAATCTTGCGTATCCGCCGATGCTCGAACGCTANCCACGCCAAACCAACCACTGTGACNACAATCGCCACCACAC
    CAAAGGTCATGCCCTCGGCGTGATGTCCGGTGCCGAAAGCCGCAAGAGCTCCGACGCCGCC
    :::::::::::::::Rv322T7.seq:::::::::::::::
    CATTCCCAATTGAATTTCCCNATCCCACAATCTCGGTTCAGATACAGGTCGCCATACCCCTTACTTCGGCAACGCTGGGCGGATTGGCCCTGCCGCTGCAGCANAC
    CATCGACGCCATCGAATTGCCGGCAATCTCGTTCAGCCAATCCATACCCATCGACATTCCGCCGATCGACATCCCGGCCTCCACTATCAACGGAATTTCGATGTCG
    GAGGTCGTGCCGATCGATGTGTCCGTCGACATTCCGGCGGTCACCATCACCGGCACCAGGATCGACCCGATTCCGCTGAACTTCGACGTTCTCAGCAGCGCCGGAC
    CCATCAACATCTCGATCATCGACATTCCGGCGCTGCCGGGCTTTGGCAACTCGACCGAGCTGCCGTCGTCGGGCTTCTTCAACACCGGCGGCGGTGGCGGCT
    Clone Rv327
    :::::::::::::::Rv327SP6.seq:::::::::::::::
    CTCAAGCTTTCGGCGGAGACGGACANNTTGCGAACATTGATGACAAAATAGAAATCATTGATGGTTTGAGTCACCAGGCCGATCAAGCCTTCGCCGAGCCAAATTC
    CAATCAAGAGGCCCAAGCCCGTACCAATCAGCCCGGCAACGAGGGATTCCGTCATTATCAGCCAAAATAACTGCTCTCGGGTTACACCCAAACAGCGCAATATGGC
    GAAAAACGGTCGCCGTTGCAGCACATTAAATGTCACGGTATTG
    :::::::::::::::Rv327T7.seq:::::::::::::::
    AGCTTAACTGCTCCCTAATACCTGGGGCTGTGCCTGCGGTGTATGCACGGCATACGGACATCCNTCCCCTGAGACCCNCGGTCTAATCAGCCACGTGTCCACCATC
    AGGGGTCAACCCCGGCCAAGGGCGACGGCACCCCAAGTTCGCCGACCGTTAACCTATTGCTGTGAGCTTCATTTGCTGCGAGCAAAACAGTTGGTCGGCCGTTAGG
    AACTGAATTGACACTCAACCGATTTGGTGCCNCCGTAGGTGTCCTGGCTGCGGGTGCGCTGGTGTTGTCCGCGTGTGGTAACGACCACAATGTGACCGGGGGAGGT
    GCAACCACTGGCCACGCGTCCGCGAATGCTATTGCGGGGG
    Clone Rv328
    :::::::::::::::Rv328SP6.seq:::::::::::::::
    CTCAAGCTTGGGGTGGCGCTGTCGGTCGGTGTGCTTGGCGGCGTCGGTATCAACACCGCCCACGAAATGGGCACAAGAAGGATTCGCTGGAGCGGGTGGCTGTCCA
    AAATCACCCTCGCCCAGACCTGCTACGGGCACTTCTACATCGAGCACAACCGTGGCCATCACGTCCGGGTGTCCACACCGGAGGACCCGGCGTCGGCGCGGTTCGG
    CGAAACGTTGTGGGAGTTCCTGCCCCGCAGTGTTATCGGCGGCTTGCGCTCGGCCGTTCATTTGGAGGCCCAACGGCTGCGTCGGCTCGGCGTCAGCCCCCT
    :::::::::::::::Rv328T7.seq:::::::::::::::
    GCACCAAGGCCCCACACGTCACCCTGTGACCTCCTGCGCCGACCCGCCCCGAGGTCCTGGCCGTTACAACCTGAACGGGCGAGCCCGGAGTCTGGTACGCATCGAA
    CAAAGAGCAAGGTGCATGGGCGGAGTTGTTCCGCCACTTCGTCGATGACGGGGTCNATCCATTCGAGGTCCGTCGCCGCGTCGGTCGAGTGGCGGTCACACTCCAG
    GTACTCGACCTCACAGACGAGAGGACTCGATCCCATCTAGGTGTGGACGAAAACAGTCTTCTGTCCGA
    Clone Rv329
    :::::::::::::::Rv329ST6.seq:::::::::::::::
    TCGCCTCCGCATATGGGTCGACGCCAAGCGGGTCCGGATTTTCTGGGCTTCATCGCTCGCGCCGTCGCGACAAACAGCGCGGTCGACCGACACTCGTTGTGATGTC
    CCAGCTATCACCTTCGGTACGCACCCATCGACCCTACNCGGCTATCTCAGCCGCGATCTCCAGGCTCCGCCGACGGAGGTGCATCCCGGTCCGGATCCCCACTAAC
    CCGGCACCATTGGCGTCN
    :::::::::::::::Rv329T7.seq:::::::::::::::
    GTCCTCGAGTGCCGCCGTCGNCACNCCCAGCGCCCGCGCGGCCACTTGGATGCGACCCGTTTCAAGTCCCTTCATCATCTGCGAAAAGCCTTGACCCATGGCTCCG
    CCCAGGATCGCCGAGACCGGCACCCGGAGGTTGTCGAACGACAGCTCGCAGGATTCGACGCCCTTGTAACCCAACTTCGGCAAGTCCCGCGACACCGTGAGTCCCG
    GCCCGGGTTCGACGAGCACGATCGACATGCCTTGGTGCCGCGGTGTGGCGTTCGGGTCGG
    Clone Rv32
    :::::::::::::::Rv32SP6.seq:::::::::::::::
    GGCATACCAATGTGGACTTCTGCTCACCCACGATATCCGTGGTCTGATCCGCTGCTGCGGCGGGCTGCNACCTGCNTTCTCNGCGGCACCCGTNACTACATGCNCG
    CGCCGCACGCATACGTCGCGGCGGGACCCACTCCNACTGGTCGACGGTGCTGGCCGCGTGTCCGCANGTCCCNAACCCGGCCGCACCCGACGAAACCGGCCGCGTC
    CGTTCTGGAGGCCAACGCTCATGTGCCGTCGGGTCCATGCTCGACGCCATCGAGACCGTAACCAGCGTCCTCGAGCGGTCGCCTCCGGCTTCCGTGACATCTTCGT
    GGCTGCTCGCGCCGTGCCGCCGCGCGGATGGTCGACCACAACGCCAACCACCTCGGCGGTGACATCACCGTCCGCGCCACTCGACCTGCGCGCCGATCGCGGCCC
    :::::::::::::::Rv32T7.seq:::::::::::::::
    GTGAGCAGACCTACGCCNCCTGGTTGCGCCAACTCGGTACCGATCATGGCGCGCNGCCTGTCGTCACCGATACCCAGCGAACAAGACAGCCCGGTCCGCGACAAGA
    TGACTTTCCCGATCTCTTCGGCGACTTCCATGGGGTCGTCCGGAAGTCCCGGGCGCCACCGCGAGGTAACCCTCGTCTCAGTCCCATACGCGACCGGGTATCACGT
    CGCGCAACAACGCCACCACCTCCCAGACGCCNCGTTGTACGCGGCTGGGTTCCACNGCAATAAGTGGCCTCANGGCATCGTCCGGCGGCGGTCCNCAAACGCA
    Clone Rv330
    :::::::::::::::Rv330SP6.seq:::::::::::::::
    CTCAAGCTTGAGGTTAACTTTGAACGGATCGAGCTGGACGTTCGAGACGGTGATCGGGCCGAACCTGAATTGTCCGGTAATGCCCAACGCAAAAAGCAGGGTGGTG
    GCCGGGGCGGTGAAACCGGCGTCGGCGGCACCGTCGAAATCTATGTGGATTGCCGGAATGGGATGTCCGGCACACGGCGAAACCGTAGTTCGCTTGTCCCGTGAGC
    CCAGGTGGATGGGGGAAAGATCCTGGTGTCCGGGATAATAATGGGCCGATGCCGCCGGTTGAAGCTCCACTGGATCGGGAATTCCGGAATCTTGATCCGACGCTTC
    AGGCCGAACAGGCCCCTC
    :::::::::::::::Rv330T7.seq:::::::::::::::
    CGGCGACGTCGCGATACGCCGAGCAGTTGGGAATCGCTCTGCAGCAAACCAATATTCTGCGCGACGTTCGAGAGGACTTTTTGAATGGACGGATCTACCTGCCGCG
    CGACGAGCTGGACCGATTAGGCGTACGCCTCCGCCTGGACGACACCGGGGCACTCGATGACCCCGACGGACGGCTCGCGGCNCTGCTGCGGTTCAGTGCCGACCGC
    GCCGCAGACTGGTNTTCGCTGGGACTGCGGCTGATTCCACACCTCGACCGCCGCAGCGCTGCCTGCTGTGCGGCCATGTCTGGCATCTACCGCCGTCAGCTCGCCT
    TGATCAGAGCATCGCCGGCGGTCGTCTA
    Clone Rv331
    :::::::::::::::Rv331SP6.seq:::::::::::::::
    CTATAAAATACTCAAGCTTGATGCCGCCGAAACCGAGCGTGAGCACGCCGCCAGGCACCAGGCGCGGGTCCGGGGCCGGCCCCCCCCCGGCCCCCTGCTCCGCTCG
    GTGATGGCACGCCACCGCGACACCACCCGGNTGCGCTACGTCNAGCCATACCGGGCGGAGCTACATCGGCTCGGCCGCCCAGTGTTCGGGCCTCTTTCGAAGGTCN
    AGGTCNATACCGATTTGCGCATCCGCAGCCGCACCCTGAACNACANAACCGTGCCCTACTATTGCTTGTCNGGCGGGGCCAAAAAACAGCTTGGCATCCTGGCCCN
    ATTGGCCGGCGCGG
    :::::::::::::::Rv331T7.seq:::::::::::::::
    CTTCGGTCGCAGTGTGCGAGTGATAGATGACGACCGGGACCTCGTCGGCATCTTCCATAGCCCCGCACACCTTCAGTTGCTCACCGGAATCCAACCGGTAGAAGGT
    CGGCGAGCGCTCGGCATTGGTCATCGGGATATGCCGCTCGGGACGGTCAGAGCCCTCGGGTCGGCCAGCACTCCGCAGCGCTTCGTCGGGGTGGTCCCCGAGCCCA
    TGGCCACCATCGCATTCACCAGGTCTGCGCGAATCNCCANCACGTANACNGTTCCTTTCCTAA
    Clone Rv333
    :::::::::::::::Rv333SP6.seq:::::::::::::::
    CTGGCACCAAGGCCCCACACGTCACCCTGTGACCTCCTGCGCCGACCCCGCCCCGAGGTCCTGGCCGTTACCACCGAACGGGCGAGCCGGAGTCTGGTNCGCATCG
    AACAAANAGCAAGGTGCATGGGCGGAGTTGTTCCGCCACTTCGTCGATGACGGGGTCNATCCATTCGAGGTCCGTCGCCGCGTCGGTCNAGTGGCGGTCACACTCC
    AGTACTCGACCTCACAGACNAAAGGACTCNATCCCATCTAGGTGTGGACNAAACAGTCTTCTGTCCGACNACTACACCACCACCCAGGCCATCGCCGCCGCCCGGG
    CGATGCCAACTTCGACGCCGTACTGGCCCCGGCCGGGGGGTCCCCGGTTGTCAAACACTTGCCGTGTTCNTTCACGCNCTGCCCCACATCCAACCCCCAACG
    Clone Rv334
    :::::::::::::::Rv334T7.seq:::::::::::::::
    GTTCTTGGCCCATGCGGAGGTATCGCCGTTTCCACCACGCGGTCGGGGTGGCGTTGCATTAGCTCACCGATGGTGCGCTTGTGCAGGCCGCCGGGATACCCCCGAG
    TGCCGGTAAACCATCTTGTGCTGC
    Clone Rv335
    :::::::::::::::Rv335SP6.seq:::::::::::::::
    CAATACTCAAGCTTGGCGTGCCGTTCCAACCCGAATTGGCTTTCGGCGCCATCGGTGAGGACGGCGTGCGGGTGCTCAACNACNACGTCGTCCGCGGGACACACCT
    CGATGCTGCCGCCATGGACGCGGTCGAACGCAAGCAGCTGATCGAGCTACAACGCCGCGCCGGAACGTTCCGCCGCGGGCGTGACCGCATCCCGTTGACCGGGCGG
    ATC
    :::::::::::::::Rv335T7.seq:::::::::::::::
    CNTCATGATGATCATCACCCGAAGTGTGGTAGCCGCAGTGGTTATCGTGGGTACCGTCGTGCTTTCCATGGCGCCTCTTTCGGGCTTTCCGTATTGGCTCGGGGAG
    CACATTCTGGGTATCGAGTTGTACTGGATGGTGTTGGCGATGTCGGTGATCCTGCTCCTGGCGGTGGGATCCGACTACAATCTGCTGCTGATTCCCCGGTTGAAAG
    AGGAAATTGGGCCGGATTGAACACCGGAATTATCCGTGCCATGGCTGGTACCGGGGGAGTGGTGACGGCTGCCGGCATGGTGTTCGCCGTTAGCATGTCGTTGTTT
    TGTGTTCAGCGATTTGCGAATTATTGGTCAGATCGGTACCAC
    Clone Rv336
    :::::::::::::::Rv336SP6.seq:::::::::::::::
    ATACTCAAGCTTTTACGGTGATCGCNCATCACCTGGTTCATGAACTGGAAGCAGCCCAGCGCTTCCTTTTCGGCCGCAACATGAGCCAGCCTCTCGTCGGCGGTCG
    GGTGCAGGTGCTCGGGCAGCTCGGCCGCNACAGCCGCCTGACCCTGAAACCAGCTTCCATATCCCGCGANNAACGACGCCAGTCCGCTACGTNACCCCTCCCGGAC
    TGTCCATGGAGAACAGCGCGTTCTCCACCGACCGGGCCCGGGTGTGGGGTNTT
    :::::::::::::::Rv336T7.seq:::::::::::::::
    GCTGGTAGAGTCGCTGACCGGTGCAGGTTTCGACAATGTGGTGCCGGTTCGGCGGCTACGTGCCATCGAGACACTGGCGCAGGCTATCGCACCCGTTATCGGCTAC
    GAGCAAATCGCGGTATGCGTTCTTGAGCATGAGTCGGCGACCGTCGTCATGGTCGACACCCACGACGGAAAGACGCAGATCGGCGTCAAGCATGTGTGCCCCCAAT
    TATCAGGACTGACCTCCGGCTGACCGGCATGTTTGGTCGCGATGCCTGGGCG
    Clone Rv337
    :::::::::::::::Rv337SP6.seq:::::::::::::::
    GCTTTCCGCCGATACCCGCCATGTCNCGCACATCCAGGACTTCTGGGGGGATCCGCTGACAGCGGCGGGATCCCAAGTGCGGATGATCGGGCCCCCGTACGTCGTG
    GTGTACCTCGTCGGTAACAACGAAACCGAAGCGTATGACTCGGTCCACGCGGTGCGGCACATGGTGGACACCACACCGGCCACCGCACGGGGTGAGGGCTATGTCA
    CCGGTCCGGCANCACTCAATGCCGACCAGGCCGAGGCCGGANACAAAAANTATCGCTAAGGTCACCGGATCACNAGCATGGTGATCGCAGCAATGTTGCTAGTGAT
    CTACGCTCCGTAATTA
    :::::::::::::::Rv3337T7.seq:::::::::::::::
    CTTCCAACCCGAATTGGCTTTCGGCGCCATCGGTGAGGACGGCGTGCGGGTGCTCAACGACGACGTCGTCCGCGGGACACAACCTCGATGCTGCCGCCATGGACCG
    GTCGAACGCAAGCAGCTGATCGAGCTACAACGCCGCGCGGAACGCTTCCGCCGCGGGCGTGACCGCATCCCGTTGACCGGGCGGATCGCGGTGATCGTCGATGAGC
    GCATCGCCACCGGAGCGACGGCCAAGGCGGCGTGCCANGTCGCCCGGCGCACGGTGCGGACAAGGTGGTGCTGGCGGTCCCGATCGGCCCCA
    Clone Rv338
    :::::::::::::::Rv338SP6.seq:::::::::::::::
    TACTCAAGCTTCGCGAGATCCGGATGGCACTCACGCTGGACAAGACCTTCACAAAATCTGAAATCCTGACCCGATACTTGAACCTGGTCTCGTTCGGCAATAACTC
    GTTCGGCGTGCAGGACGCGGCGCAAACGTNCTTCGGCATCAACGCGTCCGANCTGAATTGGCAGCAAGCGGCGCTGCTGGCCGGCATGGTGCAATCNACCAGCACG
    CTCAACCCGTA
    :::::::::::::::Rv338T7.seq:::::::::::::::
    CCCACGACTTTCTCCTCGATCAGTTGGATTTGTACGAAGAGGCAACGAAAGCAGTGATCCTCGGGATGGTCGACGCCTACATCGACCCCCGTTCGCCGCCGCACAG
    CCTGCTAGATGCGCTGGGCGAGCAGGTCCCACAGTTCGCCGCTAAGGCACGGCGTCTGTTCCCGTCCGGATCGCCATTCGGCCTCGGCGTCCTGCTCCCATTGGAT
    CAATAGGGCTGGCAGCTCCGTCGGCAGGGGCCTACGCCTCACCCCGTCACG
    Clone Rv339
    :::::::::::::::Rv339SP6.seq:::::::::::::::
    CTCAAGCTTATGCGCGCCGGCCGAGGTCTGCTCACGGCAACCCCTGAAGTTTAGGGGACNACCTACTCAGCGCAAAATTTCGCTAATGTGAGTCCGGCCCACCAGG
    GGNANATCACCCATGTCGATCATGATCTACCCCGGATACCGGATTGGCGGTAGCGCCCACGATCGTCNAAATNTCCGCCTGAATCATCGGATAGCTGATCGGGGGT
    CACGCGTTTTGANTTCACCGCGAACAGCCGCCAGCCGGCCCCGCANCGANCCGATCTCNTGCCCGGCCATGGGCCCCCAATCTTNTCG
    :::::::::::::::Rv339T7.seq:::::::::::::::
    GTGTGTGGTGGAACCCATCTGAGCAGTGTGCCAAACCGGGGCAGACAGCTCCCAATTGACGTGAGCCCCGCTCACTTGCTGGGTAAGCGTC
    Clone Rv33
    :::::::::::::::Rv33SP6.seq:::::::::::::::
    CTTTACACTTCCTGCATCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATTTAGGTG
    ACACTATAGAATACTCAAGCTTGGCGTGACGGCCACCGGGCCACTCCGCACCATCTGTACCGACCCAAGATCTAC
    :::::::::::::::Rv33T7.seq:::::::::::::::
    CAGGCATGCAAGCTTTAGCTGCCCGAATGCGTCACCCCGATGCGCCCAGATCGGGGCTTCGCAGATAAAGCACGAACAGGCGGGCAAAACGTCNATCTCGGAGCCG
    GAAGGGCAATCAGCCGACCGTCGACGAACGACACCGGCGAGACCACTTAGGCAGTGACGGCCGGCCCGAACATTACGCGCTCGTTGATTAGGCGTTCGGTCTCGTC
    CGCGGTCATGCCGAGCAGCTTGCGGCAGATCTGAACGCTGTCCTGTCCGGGCAGCGGCGCCGGGCGTTGGGTGCCTGCCCGAATGTGACGAAACCCGGAGCCGACC
    CGTCTCGGCGGGCCGCGGACGGCGATCCGC
    Clone Rv340
    :::::::::::::::Rv340SP6.seq:::::::::::::::
    CNCAAGCTTGCGGATGTTACCCCTGACAGCCTGAACTATGTCNAAAACACACGGCACCCGGAACGGTGTTGGGGACCCCATCGANTTCGAGTCGCTGCCGGCACTT
    ATGGCCTGGGTAAAGGCCAGGCNANAGCCCGTGCGCATTGGGGTCGGTCAAAACCAACATCGGCCACCTGGAGGCGGCCGCCGGTGTGGCTGGATNCATCAAGCGC
    GGTGCTGGCGGTGCAACGTGGGCACATTCCCCGCAACTTGCACTTCACCCGGTGGAACCCGGCCATCNACGCGTCGGCNACGCGGCTGTTCGTGCCNACCNAAAAC
    CCCCCGTGGCCGGCGGC
    :::::::::::::::Rv340T7.seq:::::::::::::::
    GGAACCGGTAACCAGATCAGCTCGTCGACCTCACTGCCGGGGGTGAATTCCCCACCGGTGCTGCGCGTGCCCAGTAGTGCACCTTCTTGACGCCTCGAAAAGGGGG
    AGTCGGTCGGGTAGGTCACCGTCAGGAGCCGCCTACCCAGGTTGGCGCNATAGCCGGTCTCCTCGAGTATCTCCCGCACCGCCCCCAGCCGTGCGGTCTCACCCAN
    ATCCACTTTGCCCTTGGCAGCGACCAGTCGTCGTANCNGGGGCGGTGAATGACAACGATCTCGACCGGCCCTTCCN
    Clone Rv341
    :::::::::::::::Rv341SP6.seq:::::::::::::::
    TACTCAAGCTTCAGAACAGGCCTGTTGTGGGCNCACCCGGCTCGCCGAGTTCTGCACGCACCGGCCTCAAGTGCGGCCCGCACCGCCGGCATCTCCCGGTCACCAG
    GGCCGGGCCCGCGCCGCAGCGACGGGCGTGTTCGCGCAGTTCGCCGTCAATGATGCTGACCTGATCGGCCACCCGGGCGTTCTCGGCGTCGTCGCGTTCACTAATC
    GCGGTGCTCAGCAGCGTCTCGACAGCCACCACCCGAGTGGCGACCAGCTGC
    :::::::::::::::Rv341T7.seq:::::::::::::::
    TAATGTCTTGCCAACGTCACCACAATCGCGATGAATTCAATCATGCCGCCCAGGGCGGCCAACCCAATGGTGGCCGCGAGCGGCAGCTCGATCGCAGCGGGGAGGT
    TGCCGGCCGCCAGTTGATTCACGAACAGGGTGAGGTCATAGGCGGGCAGGATAGTGACGAAGGCAAGACCTATATCTGCCGTCGGAAGAAGAATCGAGTAGCCGGT
    CGACACAACGGAAGCGAAAGTGTCCGCGATGTTGATGAGCGTGCCGGTTGTGGCGGCGGTGGCGGC
    Clone Rv343
    :::::::::::::::Rv343SP6.seq:::::::::::::::
    TACTCAAGCTTTCGTCAGTTCATCGCGCCAGCAGCCAACAAGAGCATCGGGACATACGGAGTCAACTACCCGGCCAACGGTGATTTCTTGGCCGCCGCTGACGGCC
    GCGAACGACGCCAGCGACCACATTCAGCANATGGCCAGCGCGTGCCGGGCCACGAGGTTGGTGCTCGGCGGCTACTCCCAGGGTGCGGCCGTGATCGACATCGTCA
    CCGCCGCACCACTGCCGGCCTCGGGTTCACGCAGCCGTTGCCGCCCGCAGCGGANNATCACATCGCCGCGATCGCCCTGTTC
    :::::::::::::::Rv343T7.seq:::::::::::::::
    CCACCCGTGTAATTTGGGATGGGCNAAAAGGCNAAGCACCGCGTGGCCACGAACGCCGGGAGGGACAATCTCGGGCGGCTAGGGCTTCTCGCGGGGAAGGCCGAAC
    GTACGGCGTTTCAACACGTCGCGTCGCCCTCCGACCGCGAACATTCGGGGATGGCAGCAACCTGGTAGCACCCTGGCCGGGCGATGATCTGCAGCGTCGCCCGCGG
    TAGTCGCCGCCCGGGCGGCTACAGTCTGAAACGCGATGACCATCGATGTGTGGATGCAGCATCCGACG
    Clone Rv344
    :::::::::::::::Rv344SP6.seq:::::::::::::::
    TCAAGCTTTAGCTGCCCGAATCCGTCANCCCGATGCNCCCAGATCGGGGCTTCGCANATAAAGCACNAACAGGCGGGCAAAACGTCNATCTCGGAGCCGGAAGGGC
    AATCANCCGACCGTCNACAAACGACACCGGCGANACCACTTAGGCAGTGACGGCCGGCCCCGAACATTACNCGCTCGTTGATTAGGCGTTCGGTCTCGTCCGCCTC
    ATGCCGAGCAGCTTGCGGCANATCTGAACGCTGTCCTGCCGGGCAGCGGCGCCGGGCGTTGGGGTGCCTGCCGGAATGTGACNAAACGGAGCCGACCCCCTCTCGG
    CG
    :::::::::::::::Rv344T7.seq:::::::::::::::
    CCGGGGCCACTCCGCACAATCNGTACCNNACCAANATCTACACCATCGGAATACGACGGCGTCGCCGANTTTGCGGGTACCCGCTCAACTTTGTGTCGACCCTCAA
    CGCCATTGCCGGCACCTACTACGTGCACTCCAACTACTTCATCCTGACGCCGAACAAATNGACGCNTCGGTTCCGCTGACCAATACGGTCGGTCCC
    Clone Rv346
    :::::::::::::::Rv346SP6.seq:::::::::::::::
    NCTGGCCTTTGGTCCACACTAANACAATACTCAAGCTTCCCGGCCGCAGAGCCGCCAACTCACGATATCGTTAACCGATATCCCGAGCCGATAGCTGCCGGCTCGG
    GTGGTGGCCAGCGGCGCTGCGACNAAAGGTGTGACCGTCATGAACAGACACCACCGGCCGGCCGTCGGCCGTCGTCACCTGCTCGANATCTCAGCATCCGCAGCCG
    GTGTGATCGCGCTTTCGGCGTGTNGTGGGTCNCCGCCCGAGCCCGGCAAGGCCGGCCCCGACACAACCCCGGAAC
    :::::::::::::::Rv346T7.seq:::::::::::::::
    CATCTGCCCACCACACGGACCGCGGTGCGGACGCGGCTGACGCGCCTGGTGGTCAGCATCGTGGCCGGTCTGCTGTTTGTATGCCAGCTTCCCGCGCGCAACTGCT
    GGTGGGCGGCGGTGGTTGCGCTCGCATTGCTGGCCTGGGTGCTGACCCACCGCGCGACGACACCCGGTGGGTGGCTGGGCTACGGCCTGCTATTCGGGCTGGTGTT
    CTACGTCTCGTTGTTGCCGTGGATCGGCGAAGCTGGTGGCCCCGGGCCCTGGTTGGCACT
    Clone Rv 347
    :::::::::::::::Rv347SP6.seq:::::::::::::::
    GACAATACTCAAGCTTGACTGGCCACCCACCGGCATGACCACCCGACAGGCCCGACTGGTCGTACCACTCGAACGCCGGGGTGTTGATGTCCCAGCCGTGAANTCG
    TCCTGCGCGCGCAGGCCGTCNAACAGGTACAGGGCGGGCGAATTGGCACCACCACTTTGGAATTGGACCTTGATGTCACGGCCCATCGACGGCGACGGCACCTGCA
    GGTACTCCACCGGCAAGCCCGGCCGGGAAAATGCCCCGCGGTCNCCGTGCCACCGACGGCGCCGANCAAACCCGACACTAGGGCCGCGCCNACGGCCCCCGACCAC
    NANTCNACGCGACATACCCGTGACGGCGCCACNAACCCTGTCAACA
    :::::::::::::::Rv347T.seq:::::::::::::::
    CCTCCAACTCGGCGGGGAAGCGACNCCAGCCTACCGAGCTTGGAGTCCANGACGCCAGCGGCGGCGTCGGTCGCGTCGTGGTGCCGCCGGCGGTGGCGTTGGCTGG
    CAACGATCTCCACCCAGCCGGTCGGGTTACCCACGATCTCGGCATANACGCGGGCCGAGGCCCGGTGCGATACCGTATTGCGTCAATTGGGACCGGTTGTGCATTC
    GCTAGCTCGGTTGCCACACCCGTCAGGGGTTCGACGTTGGCGGGTTCGGCGGCCCCCANCACCGCTGTCACCATGCCCGCCAAGCCGACCTGCGGCGCCCACCAAC
    TGCAGCACCANCATGTCGCCGTCGCGCGCCGCGATCACATGG
    Clone Rv348
    :::::::::::::::Rv348SP6.seq:::::::::::::::
    CTCAAGCTTTTTGAGCGTCGCGCGGGGCANCTTCGCCGGCAATTCTACTANCGAGAANTTCTGGCCCGATACGGATCTGACCGAANTTCGCTGCGGTGCANCCACC
    TCATTGGCGATGGCGCCGACNATGGCGCCTGGACCGATCTTGTGCCGCTTGCCGACGGCGACGCGGTAGGTGGTCAAGTCCGGTCTACGCTTGGGCCTTTGCGGAC
    GGTCCCGACGCTGGTCGCGGTTGCGCCGCNAAAGCGGCGGGTCGGGTGCCATCAGGAATGCCTCNCCGCCGCGGCACTGCACGGCCAGTGCCGCGGCGA
    :::::::::::::::Rv348T7.seq:::::::::::::::
    CNCCAGCTTGATTGGTCTGGTTGCATTGGCCAGCTGCGCGAGCCTGGCTCACTTCAACTACGACGACCGCAAAACAATTGCCGCCTTCGGATCCGAGTTCGGTTGG
    TACGCGGCAATGGAGCACCATTTCTCGGTGAATCAGACTATTCCTGAGTACTTGATCATCCACTCTGCACACCGACCTGCGAACCCCGCGCCCCTTGCCGACCTGG
    AGCAGCTGGCGCAACGTGTGAGCCAGTCCCAGGCGTTGCCATGGTTCGCGGTGTGACCCGGCCAAACGGGGAAAACC
    Clone Rv349
    :::::::::::::::Rv349SP6.seq:::::::::::::::
    CAATACTCAAGCTTGACTGGGCCCGCACTTCGGCGCCACCCACACCGTCAACGCCCGCGAAGTCNACGTCGTCCAGGCCATCGGCGGCCTCACGGATGGATTCGGG
    CGCGGACGTGGTGATCGACGCCGTCGGCCGACCGGAAACCTACCAGCAGGCCTTCTACGCCCGCGATCTCGCCGGAACCGTTGTGCTGGTGGGTGTTCCNACGCCC
    GACATGCGCCTGGACATGCCGCTGGTCNACTTCTTCTCTCACGG
    :::::::::::::::Rv349T7.seq:::::::::::::::
    TCGACGGTTTGGCGGCCTTAAATGCACTGAGGTCGTCATTGACCCCACAGCGGAAATGCCGACTATTCGCAGGCCTCCTTCGGCTTGGCTGCCGGAGAGGGCGCTC
    CGCGGGAACCGCATGCAGGTATATGACCTCGGTTTCTCGGGTGCTACCGCGTGCCTTGNTANGATNANCTCGGCGTTGGAATTGTCCAGCCGGCCCCAATTGATCG
    AGCGCANATTCGTACACNTGGCCGGCGGCGACATACGCTTCACCGTGGATCTGTCTCCACACGGACCGCCCTGCGGGATCCTGCTCACGGGTAANGGAACTTACGT
    GGCACTCGG
    Clone Rv34
    :::::::::::::::Rv34SP6.seq:::::::::::::::
    GACCACGCCAGGCTAATCACGTGACGCTACCGAATACCTNCCTAGTGGTGCAGGCTCCCGCTGGAAATGGCCCTGTACCAACTCGCGCACCGGTGCCAG
    :::::::::::::::Rv34T7.seq:::::::::::::::
    CGGCACCCGACCCCTTTGAGCCGTCCGCCGTGGCCGCGGTGGAACTGCCGACGAGGGACTGATCGTGCTGGGCAAATTGGTCGATGGCACGCTGGCCCCCCGATCT
    GAAGGTCN
    Clone Rv350
    :::::::::::::::Rv350SP6.seq:::::::::::::::
    CTCAAGCTTGCCGTTACCCCGACTTCCGGAGGGACACCATGAGCACCGCCAGCCGAGCACGAGGCCAAACTCCGCCGACGCAGGCCGGTTGGACTTGTCGTGCTGG
    ACAAGGGGTTTAGCCGCCGAAGCAGTGACGTACATGGGCGAAAAGCAGTTCGCCTGTCGACCGACGGNGCNNACCGTGAGGCTAGGGAAGCGAGGAGCACATGGCC
    GCCGACCCGCAATGTACACGCTGCAAGCAAACCATCGAACCCCGGATGGCTATNCNTCACCGCCCATCGCCGCGGT
    :::::::::::::::Rv350T7.seq:::::::::::::::
    CATGTCGCGCACATCCAGGACTTCTGGGGGGATCCGCTGACAGCGGCGGGATCCCAAGTGCGGATGATCGGGCCCGCCTACGTCGTGGTGTACCTCGTCGGTAACA
    ACGAAACCGAAGCGTATGACTCGGTCCACGCGGTGCGGCACATGGTGGAACACCACACCGCCACCGCACGGGGTGAAGGCCTATGTCACCGGTCCGCAGCACTCAA
    TGCCGACCAGGCCGAGGCCCGGAGACAAAGTATCGCTAAGGTCACCGCGATCACGAGCATGGTGATCGCAGCAATG
    Clone Rv351
    :::::::::::::::Rv351SP6.seq:::::::::::::::
    ATACTCAAGCTTCGGTACGGTGGCGGGCCGTGCTGCTGGCCGCGGTCGCGGCGTGCGCGGCCTGCGGTCTCGTTACNAGCTCGCGCTGCTGACACTGGCGCGCNAG
    CCTGAACGGCGGCGGGATCGTGGCCACCTCCCTGATCGTCGCGGGCTACATAGCCGCGCTGGAGCAGGCGCCTTGCTGATCAAGCCGCTACTTGCACACGCGGCCC
    ATCGCGTTCATCGCCGTGGAGGCGGTGCTGGGCATCATCGGCG
    :::::::::::::::Rv351T7.seq:::::::::::::::
    TGTCAAGTCCTTTCAGATCTCNTTTTTATGACATGACTGGAGATCTGTCTAGATTGCAGCTCCTGTGAGCGTGGGTACCGGATTCAAGCCGGTCGGTCACGCCCCG
    GTGGTACCGGCTTTGCGGCAGTGCTCGGCCTCGAGTTCGGCGATCGCGCGCGAAGTGCGTTCGCGCAGCAAGATCGCGGCCGTAATGCCGGCGATGACCGCGATGA
    CCAGCGCGATCCAGGAGAACCGTTCCAACCAGTGCTGGGCGGCCATCCCGGCGAAGTAGACCAGTGCAGTGGTGCC
    Clone Rv352
    :::::::::::::::Rv352SP6.seq:::::::::::::::
    CAATACTCAAGCTTCAAAACAGGCCTGTTGTGGGCGCACCCGGCTCGCCGAGTTCTGCACGCACCGCCTCANTGCGGCCCGCACCGCCGGCATCTCCCCGGTCACG
    CAGGGCCGCGGCCCGCGCCGCANCGACGGNGTGTTCGCGCAGTTCGCCGTCAATGATGCTGACCTGATCGGCCACCCGGGCGTTCTCGGGCGTCGTCCGTTCACTA
    ATCGCGGTGCTC
    :::::::::::::::Rv352T7.seq:::::::::::::::
    TACGCTGGCGCTGGAGGGAGCCANNTACAACATCCACGCCAATGCTCTTGCCCCGATCGCGGCGACCAGGATGACCCAGGACATCCTGCGCCCCGAAGTACTGGAA
    AAGCTCACACCCGAGTTCGTCGCACCGGTGGTGGCCTACCTGTGCACCCGAGGAGTGTGCGACAACGCATCGGTGTACGCGTCGGTGGTGGCACAGGTGCAGCGAG
    TTGCGCTGTTTGGCAACGACGGCGCCAACTTCGACAAACCGCCGTCGGTACAAGATGTTGCGGCGCGGTGGGCCCGAGATCACGATCTGTCCGGTGCGAAAATTGC
    TG
    Clone Rv353
    :::::::::::::::Rv353SP6.seq:::::::::::::::
    GCTTTTCCGTCCGTCNNCGCTCAACCGCGTGAGGCCGAAGCGGNTGGTTACCGACTCCCTGTTTGTGATGGACCACTTCTACCAACTGCCCATGTTGGGGACNCCC
    GACCAGCCGATGCTGGAGGCCTACACGGCCCTTGGTGCGCTGGCCACGGCGACCGANCGGCTGCNNTGGGCGCGTTGGTGACCGGCAATACCTACCGCAGCCCCGA
    CCCTGCTGGCAANATCATCACCACGCTCGACGTGGTTAGCGCCGGTCGAGCGATCCTCGGCATTGGAGCCGGTTGGTTGANCTGGAACA
    :::::::::::::::Rv353T7.seq:::::::::::::::
    CNGCTTTTTAATGGCCTTGACNTGGGCGNGCCGGCCACCGGGGCCACTCCGCACAATCTGTACCCGACCAAGATCTACACCATCGAATACGACGGCGTCGCCGACT
    TTCCGCGGTACCCGCTCAACTTTGTGTCGACCCTCAACGCCATTGCCGGCACCTACTACGTGCACTCCAACTACTTCATCCTGACGCCGGAACAAATTGACGCAGC
    GGTTCCGCTGACCAATACGGTCGGTCCCACGATGACCCAGTACTACATCATTCGCACGGAGAACCTGCCGCTGCTAGAGCCACTGCGATCGGTGCCGATCGTGGGG
    AACCCACTGGCGAACCTGGTTCAACCAAACTTGAAGGTGATTGTTAACCTGGGCTACGGCGACCCGGCCTATG
    Clone Rv354
    :::::::::::::::Rv354SP6.seq:::::::::::::::
    CTCAAGCTTGCCGGGAGGGTGCATGGCCGACTCGGATTTACCCACCANGGGGCGCCAACGCGGTGTCCGCGCCGTCNAGCTGAACGTTGCTGCCCGCCTGGAGAAC
    CTGGCGCTGCTGCGCACCCTGGTCGGCGCCATCGGCACCTTCGAGGACCTGGATTTCGACGCCGTGGCCGACCTGAGGTTGGCGGTGGACGAAGGTGCACCGCGGT
    TGATTCGCTCGGCCTTGCCGGATGCCACCCTGCGCCTGGTGGTCGATCCGCGAAAANACGAANTTGTGGTGGAGGCTTCTGCTGCCTGCGACACCCACNACGTGGT
    GGCACCGGGCAGCTTTAGCTGGCAT
    :::::::::::::::Rv354T7.seq:::::::::::::::
    CCGACGCCGTCGTGGCCACCAACACCGCGACCAGCACCGTGACCCGGACCGGGGTGCCGCGCGAACCGGTCTTGGCCAATTGCCGCGGCACCAAGCCGTCGGCGCC
    CATGGCGAACAGCACGCGGCATTGCCCGAGCATCAACACCATCACCACCGTGGTAAGCCCGGCCAGCGCCGACGGAGATGATGCCGCTGGGCCCAGTACACCCCCG
    TTGGCCTGGAACGCGGTGGCCAGTTTGCCGGCCGCGGCCCGGTACGGTCCGCAGTTGGGTGTATGGAACCATGCCCGACAGCACCACCG
    Clone Rv355
    :::::::::::::::Rv355SP6.seq:::::::::::::::
    TTNACTGGCCTTTGGTCCACACTAGACAATACTCAAGCTTCCAGGACATCGTCATCGCGACCAAAACCGCGAGCTAGGTCGGCATCCGGGAAGCATCGCACCGGG
    TGGCGCCGAGCGCCGCTGCCGGCAGGCCGATTAGGCGGGCAATTAGCCGCCGCGGCTCCCGGCTCCGANTACGGCGCCCCCGAATGGCGTCACCCGCTGGTAACC
    ACGCTTGCGCGCCTGGCGGCGGCCTGCCGGATCAGGTGGTAAATGCCGACA
    :::::::::::::::Rv355T7.seq:::::::::::::::
    NGACGTCTTCCATCCGCGCGTCGTTTTGGCGGGTTGGCCACAGCAGCCCGCCGGTGACGGCGACGATGCTGGGCTGGTTGCGGCCCTGCGGCACCGCGGCTTGCAT
    GCTGTTGGCTGTCTTGGGACGATCCCCCGAAATAGTCCACGCGGATCTGGTGATTTTGCGGGCTACCGCGATTACCCCGCGCGGCTCGACGAGTTTTGGCCCTGGA
    CTACCCGCGTGGCCAATCTGCTGAACTCGCGGCCGGTGGTGGCCTGGAATGCGAGCGCCGTTACCTA
    Clone Rv356
    :::::::::::::::Rv356SP6.seq:::::::::::::::
    CTTCCTCCTGAGTACCNCCCGTNTACTTTGGGATGGGTAAAAAGGCGAATCNCCGTTTGGTCACGAACGCCGGGAGGGACAATCTCGGGCGCCCGTGGGTCTCCGC
    GGGAANGCCCGAATGTACGGTGTCTCGACACTTCCCNTCCCCTCCG
    :::::::::::::::Rv356T7.seq:::::::::::::::
    GAGCATCGGGACNTACGGAGTCAACTACCCGGCCAACGGTGATTTCTTGGCCGCCGCTGACGGCGCGAACGACGCCNGCGACCACATTCAGCAGATGGCCAGCGCG
    TGCCGGGCCACGAGGTTGGTGCTCGGCGGCTACTCCCAGGGTGCGGCCNTGATCNACATCGTCACCGCCGCACCACTGCCCGCCGCCTCGGGTCACGAGCCGTTGC
    CGCCCNCAGCGGACGATCACNTCGCCGCGATCGCC
    Clone Rv357
    :::::::::::::::Rv357SP6.seq:::::::::::::::
    TACTCATGANCATCCTTTAATCANNGCTTTGCGTTTTTTTATTAAATCTTGCAATTTACTGCAAAGCAACAACAAAATCGCAAAGTCATCAAAAAACCGCAAAGTT
    GTTTAAAATAAGAGCANCACTACAAAAGGAGATAAGAAGAGCACATACCTCAGCACTTATTATCACTAGCGCTCGCCGCAGCCGTGTAACCGAGCATAGGGGAGCG
    AACTGGCGAGGAAGCAAAGAAGAACTGTTCTGTCAGATAGCTCTTACGCNCA
    Clone Rv358
    :::::::::::::::Rv358SP6.seq:::::::::::::::
    CTCAAGCTTCAGGTCAATGTGCNCCAAGCCCTGACGCTGGCCGACCAGGCCACCGCCGCCGGANACNCTGCCAAGGCCACCGAATACAACAACGGCCGCCGAGGGT
    TCGCANCCCAGCTGGTGACCGCCGAGCANANCGTCAAAAACCTCAAGACGCTGCATGACCAGGCGCTTANCNCCGCANCTCAGGGCCAAGAAGGCGTCAAACGAAA
    TGCGATGGTGCTGCACCANAAGATCGCCGAGCGAACCAAGCTGCTCAGCCNG
    :::::::::::::::Rv358T7.seq:::::::::::::::
    CATGGTGGCACTGTAGCGACGTGCTGCAATCAAGGTCATGCCCGACTCTGGTCAGCTCGGANCCGCTGACACCCCGCTAAGGCTGCTCAGCTCGGTGCATTACCTC
    ACCGACGGCGAACTCCCCCAGCTTTACGACTATCCGGATGACGGCAGCTGGTTGCGGGCGAACTTCATCATCAGCTTGGACGGCGGCGCTACCGTCGATGGCACCA
    GCGGGGCGATGGCCGGGCCCGGCGACCGATTCGTCTTCAACCTGTTGCGTGAACTTGCCGACGTCATCGTGGTCGGCGTGGGCACCGTGGCATTGAGGCGCTACTC
    CGGCGTCCGGATGGGTGTCGTCCAGCGCCAGCAC
    Clone Rv359
    :::::::::::::::Rv359SP6.seq:::::::::::::::
    TACTCAAGCTTGCGGGTGATCGCCTTGGTCAACGGCACCGTGATCGGATCGGGGTCNACCGCACAAATGGACTGGAGCTTCGGCGCAANCATCGCCTATGCCTCGC
    GGGGGGTGACGCTGACCCCGGTGACNTGTTCGGCTCGGGCACGGTGCCCACCTGCACGCTCGTCTATCACCTCGGCCACCGGAATCATTCCCGGGCTGG
    :::::::::::::::Rv359T7.seq:::::::::::::::
    GTTGGNGCCTCGTCGGCGAACAGTTCTCGCACGATTTCCGGATTAGCGGGACTGGTCACCAGTTGGGTATGCGGGAAGGCGCTGACGTTCGGCCGGATTAGCTGTT
    TGATGGACGCGGTGGTGATGTTCTGATCACGGAACTGGCTGTAATAGCCCAGGGTCGCCACGTTTCATCCGGCCCCGGACCCCGGCGCACCGAGCGTGTCGCGCAG
    GTATGCGACGTGATTTTCGCTGAAGTCCCCGTACCCGGAGAACT
    Clone Rv35
    :::::::::::::::Rv35SP6.seq:::::::::::::::
    TGCTTCCGGCTCGTATGTTGTGTGGAATTGTGANCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACACTATAGAAT
    ACTCAAGCTCCAGGTCAATGTGCGCCAAGCCCTGACGCTGGCCGACCAGGCCACCGCCGCCGGAGACGCTGCCTTTGTCACCGAATACAACAACGCCCGCCGAGCG
    TTCGCAGCCCAGCTGGTGACCGCCGAGCAGAGCGTCGAAGACCTCAAGACGCTGCATGACCAGGCGCTTAGCGCCGCAGCTCAGGCCAAGAATGCCGTCGAACGAA
    ATGCGATGGTGCTGCGGCATAAGATCGCCGAGCGAACCAAGCTGCTCAGGCCAGCTCGAGCAGGCGAAGATGCACGAGCA
    :::::::::::::::Rv35T7.seq:::::::::::::::
    CAGGCATGCAAGCTTCGGAGGCAGACCCGTGCATGGTGGCACTGTAGCGACGTGCTGCAATCAAGGTCATGCCGACTCTGGTCAGCTCGGAGCCGCTGACACCCCC
    GCTAAGGCTGCTCAGCTCGGTGCATTACCTCACCGACGGCGAACTCCCCCAGCTTTACGACTATCCGGATGACGGCACCTGGTTGCGGGCGAACTTCATCAGCAGC
    TTGGACGGCGGCGCTACCGTCGATGGCACCAGCGGGGCGATGGCCGGGCCCGGCGACCGATTCGTCTTCAACCTGTTGCGTGAACTTGCCGACGTCATCGTGGTCG
    GCGTGGGCACCGTGCGCATTGAAGGCTACTCCGGCGTCCGGATGGGTGCTGTCCATCGGCCA
    Clone Rv360
    :::::::::::::::Rv360SP6.seq:::::::::::::::
    TACTCAAGCTTGGGGTGGCGCTGTCGGTCGGTGTGCTTGGCGGCGTCGGTATCAACACCGCCCACGAAATGGGGCACAAGAAGGATTCGCTGGAGCGGTGGCTGTC
    CAAAATCACCCTCGCCCANACCTGCTACGGGCACTTCTACATCGAGCACAACCGTGGCCATCACGTCGGGGTGCCACACCGGAGGACCCCCGGCGTCGGGGGGTTC
    GGCANAACGTTGTGGGANTTCCTGCCCCGCANTGTTATCGGCGGCTTGCGCT
    :::::::::::::::Rv360T7.seq:::::::::::::::
    GGCCATCGCCACCGCNCCGCGGCGAACGCTCAAAGGCACCTACTGGCACCAAGGCCCCACACGTCACCCTGTGACCTCCTGGCCGACCCCCGCCCGAGGTCCTGGC
    CGTTACCACCGAACCGGGCGAGCCGGGAGTCTGGTACGCATCGAACAAAGAGCAAGGTGCATGGGCGGAGTTGTTCCGCCACTTCGTCGATGACGGGTCGATCCAT
    TCGAGGTCCGTCGCCGCGTCGGTCGAGTGGCGGTCACACTCCANGTACTCGACCTCACAGACGAGAGGACTCGATCCCATCTAGCGTGTGGACGAAACAGTCTTCT
    GTCCGACGACTACACCACCACCCAGGCCATCGC
    Clone Rv361
    :::::::::::::::Rv361SP6.seq:::::::::::::::
    GCTTGCGGGTGATCGCCTTGGTCACGGCACCGTGATCGGATCGGGGTCNACCGCNCAGATGGACTGGANCTTCGGCGAANTCNTCGGCCTATGCCTCGCGGGGGGT
    GACCCTGACCCGGGTGACNTGTTCGGCTCGGGCACGGTGCCCACCTGCACGCTCGTCAAGCACCTCNGGCCACCGGAATCATTCCCCGGGCTGGCTGCACNACGGC
    GACNTGGTCNCCCTCCAGGTCGAAGGGCTGGGCNAAACAANGCAGACCGTCCGGACANCGGCACTCCTTTTTCCGTTGGCTCTTCGGCCGAATCCGGACGCCNAAC
    CCGACCGGCG
    :::::::::::::::Rv361T7.seq:::::::::::::::
    GTTCTCGCACGATTTCCGGATTAGCGGGACTGGTCACCAGTTGGGTATGCGGGAAGGCGCTGACGTTCGCCGCGATTAGCTGTTTGATGGACGCGGTGGTGATGTN
    CTGATCACGGAACTGGCTGTAATANCCCAGGGTCGCCNCGCTTTCATCCGGGCCCGGACCCGGCGCACCGAAGCGTGTCGCGCAGGTATGCGACGTGATTTCGCTG
    AAGTCCCCGTACCCGGAGAACTCGAACACGCTGAGGCGCTCGTCACCGTCGTNNCGGCGACCAAGCGCGGCGAGCAACTGCGCAAAATCGTTAAGANAGGTCGAAT
    CGTTGAAATTCGGCACCACCTGCACC
    Clone Rv363
    :::::::::::::::Rv363SP6.seq:::::::::::::::
    CACAAGACAATACTCAAGCTTCAGGTCAATGTGCNCCAAGCCCTGACGCTGGCCGACCAGGCCACCGCCGCCGGANACGCTGCCAAGGCCACCGAATACAACAACG
    CCGCCGAGGCGTTCGCAGCCCAGCTGGTGACCGCCGAGCANANCGTCNAAAACCTCAAGACGCTGCATGACCAGGCGCTTANCGCCNCAGCTCAGGCCAAGAAGGC
    CGTCGAACGAAATGCGATGGTGCTGCAGCANAANATCGCCGANCGAACCAAGCTGCTCAGCCAGCTCGAGCAG
    :::::::::::::::Rv363T7.seq:::::::::::::::
    CCACCGTGCATGGTGGCACTGTAGCGACGTGCTGCAATCAAGGTCATGCCCGACTCTGGTCAGCTCGGAGCCGCTGACACCCCCGCTAAGGCTGCTCAGCTCGGTG
    CATTACCTCACCGACGGCGAACTCCCCCCAGCTTTACGACTATCCGGATGACGGCACCTGGTTGCGGGCGAACTTCATCAGCAGCTTGGACGGCGGCCTACCGTCG
    ATGGCACCAGCGGGGCGATGGCCGGCCCGGCGACCGATTCGTCCTTCAACCTGTTGCGTGAACGGTGCC
    Clone Rv364
    :::::::::::::::Rv364T7.seq:::::::::::::::
    GCTTTCCGCCGATACCNCCATGTCCCGCACATCCAGGACTTCTGGGGGGATCCGCTGACAGCGGCGGGCATCCCAAAGTGCGGATGATCGGGCCCGCCTAGTCGTG
    GTGTACCTCGNCGGTAACAACGAAACCGAANCGTATGACTCNGTCCACGCGGTG
    :::::::::::::::Rv364T7.seq:::::::::::::::
    CAACCCGANTTGGCTTTCGGCGCCNTCGGTGAGGACGGCGTGCGGGTGCTCAACGACGACGTCGTCCGCGGGACACACCTCGATGCTGCCGCCATGGACGCGGTCG
    AACGCAAGCAGCTGATCGATCTACNACGCCGNGNGGAACGCTTCNGCCGCGGGCGTGACCGCNTCCCGTT
    Clone Rv365
    :::::::::::::::Rv365SP6.seq:::::::::::::::
    GGGATGGGCAAAAAGGCGAAGCACCGCGTGGCCACGAACGCCCGGGAGGGACAATCTCGGGCGGCTAGGGCTTCTCGCGGGGAAGGCCGAACGTACGGCGTTCAAC
    ACGTCGCGTCGCCCTCCGACCGCGAACATTCGGGGATGGCAGCAACCTGGTAGCACCCTGGCCGGGCGATGATCTGCCAGCGTCCCCGCGGGTAGTCGCCGCCCGG
    GCGG
    :::::::::::::::Rv365T7.seq:::::::::::::::
    CAGCAGACCAACAAGAGCATCGGGACATACGGAGTCAACTACCCGGCCAACGGTGATTTCTTGGCCGCCGCTGACGGCGCGAACGACGCCAGCGACCACATTCAGC
    AGATGGCCAGCGCGTGCCGGGCCACGAGGTTGGTGCTCGGCGGCTACTCCCACGGTT
    Clone Rv366
    :::::::::::::::Rv366SP6.seq:::::::::::::::
    CTCAAGCTTGACTGGCCACCCACCGGCATGACCACCGACAGGCCCGACTGGTCGTACCACTCGAACGCCGGGGTGTTTGA
    :::::::::::::::Rv366T7.seq:::::::::::::::
    TTTGGTGCCCGGAATGGCGAGTCCCATTTANTCGCTGATTTGTTTGAACAGCGACGAAACCGGTGTTGAAAATGTCGCCTGGGTCGGGATTCCCTCTCCAAGCAAG
    AGTAACTGGCCCCCAAATAAGTTACTCGTCGTCTTGCAAAGACCGCTACCCCGATGCCATTATGTGTTCCTTACGCTCNNNNTTCCGGTGCGCCCATCATTATCTG
    CACCTTTGCACTGCACATTGAGCTTAGCAGCGCTCG
    Clone Rv367
    :::::::::::::::Rv367T7.seq:::::::::::::::
    GAATTNGCTTTCGGCGCCATCGGCCCAGGACCGCGTGCGGGTGCTCAACGACGACGTCGTCCGCGGGACACACCTCGATGCTGCCGCCATGGACGCGGTCGAACGC
    AAGCAGCTGATCGAGCTACAACGCCGCGCGGAACGCTTCCGCCGCGGGCGTGACCGCATCCCGTTGACCGGGCGGATCGCNGTGATCGTCGATGACGGCATCGCCA
    CCGGAGCGACGGCCAAGGCGGCGTGCCAGGTCGCCGGGCCCACGGTGCGGACAAGGTGGTGCTGGCGGTCCCGATCGGCCAGCGACATCGTGCCGCCGAGATTCCG
    CGGGTACGCCGATGAAGTGGTGT
    Clone Rv368
    :::::::::::::::Rv368SP6.seq:::::::::::::::
    TAAAGCTTTCGTCAGTTCATNGNGCCCCCGGACCAACAAAAGCATCGGGACATACGGAGTCAACTACCCGGCCAACGGTGATTTCTTGGCCGCCGCTGACGGCGCN
    AACGACGCCAGCGACCACATTCAGCAGATGGCCAGCGCGTCCGGGCCACACGAGGTTGGTGCTCGCGGCTACTCCCAGGGTGCGGCCGTGATCNACATCGTCACCG
    CCGCACCACTGCCCGGCCTCGGGTTCACGCAGCCGTTGCCGCCCGCAGCGGACGATCACNTCGCCGCGATCGCCTGTTCGGGAATCCCTCGGGCCGCGCTGCCGGG
    GCTGATGAGCGCCCTGACCCCTCAATTCGGGTCCAANACCATCNACCTCTGCAACAACGGCGACCCCGATTGTTCGGACGGCAACCGGTGGCGANCGCACCT
    :::::::::::::::Rv368T7.seq:::::::::::::::
    CCGGGAGGGACCATCNCGGGCGGCTNCGGCTTCTCTCCGGAAGGTTCTANNGTNNGCGTTCNACNCTTCCCGTCGCCCTGCGACCGCCCGAACATTCGGGGGTATG
    CNNGCANCCTGTNAGCATCCNGGCCGGGGC
    Clone Rv369
    :::::::::::::::Rv369SP6.seq:::::::::::::::
    CTCAAGCTTCCGCATCAGATCGCTATAGAACCGGTGCGCGTCCCACCGAGTGGCTGGTCGCCTTCCAGCACGATCGTTACCGCGTTATCGGAATCAAACTCNCCCG
    AACACCTGACCAACGCGCTTGATCGCCTGAATCGATGCGGCGTCGCTGGGGCTCATCGATACCGAGTGTGCTTTTCCGACCACTTCCAGTTGCGGTACGGCGAGAT
    TGACAAAGGCGGTGAAGCCCAGCCAGAGCAGGACGATCACCNCCGCAAACCCGGCGGATTTGCCCG
    :::::::::::::::Rv369T7.seq:::::::::::::::
    GCTTGGCAGCCTGCGGCTGGGCGCCCTNGAGCTCTTCGATCTGGATCTCCGGACTCGAGATGCTCACTTGCCCGGCCGTGGACGTACCCATTGCGGCCGGGACCCC
    AGCGCCCCCCAGGTGACCAGCGAGTTGGGCTGCACGCTGACCGGCCCGTCGGGTCGACGCCGGTAACGGTCAGCAGCTCCGANGTCCNCTGATCCCGACCGCAGCT
    GCCAATGCGCGCTGGCAGCCGACGTGGATGTGCCGGGGCCTAGATCGCGGGGCAGCAGCGAGACCCGCGTCACCGACGGTCATCACCTTGCCGAGTTTAGGCCTGC
    CGCAN
    Clone Rv36
    :::::::::::::::Rv36SP6.seq:::::::::::::::
    GCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTNCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATCTAGGTGACACTATAGAATA
    CTCAAGCTTGAGCCTATCGGGCTATCAGCTGGTTGATGTCCCG
    :::::::::::::::Rv36T7.seq:::::::::::::::
    CAGGCATGCAAGCTTGTCGTCTATCACATCCGACCACCAACGCCCGACGGCTCGGCAGAACGCCTCCGCATATGGGTCGACGACCAGCGGGTCGGACTTCTGGGGC
    TGCCAGCGCTCGCGCCGTCGCGACAAACAGCGCGGTCGAACCGACACTCCTTGTGATGTCCCACCTATCACCTCGGTACGCACCCAATCGACGCTAGCGGGCCTAG
    CTCAGCCCCGATCTTCCAGAGCTCCGCCCCG
    Clone Rv370
    :::::::::::::::Rv370SP6.seq:::::::::::::::
    GCTTTTTGAGCGTCGCGCGGGGCGGCTTCCCCGGCAATTCTACTAGCGAGAAGCTCGGCCCGATACGGATCTGACCGAAGTCGCTGCGGTGCAGCCCACCCGCATT
    GGCGATGGCGCCGACNATGGCGCCTGGACCGATCTTGTGCCGCTTGCCGACGGCGACGCGGTAGGTGGCAATTCCGGTCTACGCTTGGGCCTTTGCGGGACGGTCC
    CGACGCTGGTCGCGGTTG
    :::::::::::::::Rv370T7.seq:::::::::::::::
    CGANCCTGTTCGACGGCTACCTGAATCACCCCGATNCCACCGCCGCGGCGTTCGACGCCGACAGCTGGTACCGCACCGGCGACGTCGGGGTGGTCGACGGCAGTGG
    GATGCACCGCATCGTGGGACGCGAGTCGGTCGACTTGATCAATCGGGTGGATACCGGGTCGGCGCCGGTGAAATTGAAACGGTGCTGCTCGGGCCCATCCGACGTG
    GCGGAGGCGGCAGTCGTCGGGGT
    Clone Rv371
    :::::::::::::::Rv371SP6.seq:::::::::::::::
    NAAGCTTTGTCACACCCAAGTGTTTCNACCAGNCGCTCCATCCGGCGAAGTGGATACTCCCAGCAGGTAGCAGGTCGCCACCACGCTGGTCAGTGCCGTTCAGCTC
    GCTTGCGGCGCTGCAGCAGCCAGTCCGGGAAATAGCTGCCCTGGCG
    :::::::::::::::Rv371T7.seq:::::::::::::::
    CCGCTGGNCGGCGGCGCTGGGCTGCGGTAACAATTACCACAACACTTTTCGGTAGCCGAACAGCGGCGCGTACCAGCGAAATGGCACAGCCACCGCAGTCGCCGAC
    ATCCCGCGAAGATGGCAGTTTTCGTGCGGTCGAGCGGCGAAGGCCTAGCGTCATTGTTGCCTGGCAAGGTTGCTGGCCCCGG
    Clone Rv373
    :::::::::::::::Rv373SP6.seq:::::::::::::::
    CTCAAGCTTCTTCTGCCCCTTGCCGTTNCGGATNACATCCCGCAGCGACTCGGCTTCGGCGTCGATGTCGAAGTTCTCGATCAGCTTCTGGATCGACTCCGGCGGG
    ATGGCACCGGTGAAGCTACTCGCCGTAGCGGTCGACNAGTTCGCGGTAGAAGTTTTCGTCNACNATCAGCTGCTTGGGCGGGANCTTGGTGAAAGTGCTCCAATGT
    CCTCCAACCGGTCCAGCTCACGCTGCGCGGTCACGGATCTGGCGCATCTCGCGCTCGCCGCCGTCGCGAACTTGCGCCGCCATCGGGCTTGGGGCC
    :::::::::::::::Rv373T7.seq:::::::::::::::
    GTTCACACCTACCTACTATGCCNCAATTCNCCGACACGGGTGGCATCAACACGGGCGATATAAGGTGGAAATCGCTGGGTGAACGTCGGGCTGGTGCGCTCGCTGC
    AATCCGCGGCAACCGCGTGTTGATCGGATTCTCGTTGCCCGGCAAGACAATCGGGATGCAAAGCCGGGCAGCAATTCNCNCCNACACCATTCTTGGCCGTAAGAAC
    CTGGAGATCGAACCCCGCGGTTCGGAGCCGTTGAAACCCAACGGTTTCCTGCCGTTGGCGCANACCACTACGCCATACCAAATC
    Clone Rv374
    :::::::::::::::Rv374SP6.seq:::::::::::::::
    CTCAAGCTTTACGCCGACGCCGGCCTACACAACACCAAGGAAACGATTGCCTACTGCCGAATCGGGAACGGTCCTCGCACACCTGGTTCGTGTTGCGGGAAATTAC
    TCGGACACCAAAACGTCAAGAACTACGACGGCAGTTGGACAGAATACGGCTCCCTGGTGGGCGCCCCGATCGAGTTGGGAAGCTGATATGTGCTCTGGACCC
    :::::::::::::::Rv374T.seq:::::::::::::::
    TCCCNCATGGGATAACGGGTTTAGATTTCNACAACGGCACCGTGTTTCTCAACAAGCCGGTCATCAGCTGGGCCGGCGACAACGGTATCTACTTCACCCGCTTCCG
    CCCGTACAAGAAAAACCACTAGGCCACCATCGAGTCCAAGAACAACCACCTGGTCCGCAAGTACGCGTTCTACTACCGCTATGACACCGCCGAGGAACGCGGCGTG
    CTCAACCGGATGTGGAAGCTGGTCAACGACCGCCTCAACTACCTCACCCCGACCATCAAACCGATC
    Clone Rv375
    :::::::::::::::Rv375SP6.seq:::::::::::::::
    CTCAAGCTTGGGTGTTGCCGATCACCGGAAGCCNCATGATCAGCCAGTTTCGCGCCGCCCGGCATACGGCGGCGTACCGATCTCCGCGTCATACACCCCGGCGGTA
    ATCGCCGACGGTGCCGGTTCGCGAGCCGAAGGTGACAACGCTGATTGAATCNAGTTCCANGTCCAGCGGGT
    :::::::::::::::Rv375T7.seq:::::::::::::::
    TNAACAGCTCGCGGCAGCCCACGACCTGCTGCGTCGGATTGCCGGCGGCGAGATCAATTCCAGGCAGCTCCCGGACAATGCGGCTCTGCTGGCCCCGCAACGANGA
    CTCGAGGTCACCCCGGTGCCCGGGGTCGTGGTGCACCTGCGATCGCACAGGTTGGCCCACAACCGGCCGCTTGATGNNNNGTCGGCAAGCCCCGGCAGTTNGCCAA
    CCCAGCGTGATCANGCTCGGCTCGCGAGTTCGGCGAAANAAGTGGCTCGCCTGATCACCTACCATCGGCCANGATCTGCTGTCA
    Clone Rv376
    :::::::::::::::Rv376SP6.seq:::::::::::::::
    GCCANACCGGCTTGGCGTCGACTCCCGTTCNGCACATCATACGGTCCCCGGTACTGTCCAACTGCGCCGGTGCGCTAGCCAAACGTCACGACTCTCAGTGTCCCAG
    TTCGTGATCCGGCCGGTGGCGCCGCTGCGGCGGGGCTNATNTACTTCGGACTNATTATCTCATCCAAAGGACACGGGGCCGGTGGCTGGAATCCCATGGTGCCGAT
    CGGCCACACAN
    :::::::::::::::Rv376T7.seq:::::::::::::::
    CCGACCTGGTATCTTCCGATAGCCGCGTTGATATCCGGTCTGATCTCCTGCCCTTAACGCCCGATCTCAGCAGGTCCCCATGCAAAGATCCGAAGGTGTCCCNGAT
    CTAGGGGTCCTCGTCCTCCAGATGATGGAGCAAGTCGGCCC
    Clone Rv377
    :::::::::::::::Rv377SP6.seq:::::::::::::::
    CTCAAGCTTCGGCTCAGGCGGCGCTGCCGGTAACGTCGCTGACCGGTGCAGGTTTCGACAATGTGGTGCCGGTTCGGCGGCTACGTGCCATCAAGACACTGGCGCA
    GGCTATCGCACCCGTTATCGGCTACAAACAAATCGCGGTATGC
    :::::::::::::::Rv377T7.seq:::::::::::::::
    CATCACCTGNTTCATGAACTGGAAGCACCGCAGCGCTTCCTTTTCGGCCGCAACATGAGCCAGCCTCTCGTCGGCGGTCGGGTGCAGGTGCTCGGGCAGCTCGGCC
    GCGACAGCCGCCTGACCCTGAAACCAGCTTCCATATCCCGCGACGAACGACGCCAGTCCGCTACGTAACCCCTCCGCGACTGTCCATGGACAACAGCGCGTTCTCC
    ACCGACCGGGCCCGGGTGTGGGGTGT
    Clone Rv378
    :::::::::::::::Rv378SP6.seq:::::::::::::::
    AGCTTAGCTTCCCGCCCCGGCAATAGGGCTCCAGCTCATCCGGTGTGACCAGATAGGGGCCCAGGTGATACCGCTGTCTTTGCCCTTGGCCTGTCCGATGCGCCAG
    CTGGCCCTCCAGCATCTGCAGGTCCCGTGCGGACCAGTCGTTGAAATGGTATAGCCGATGATCGACCG
    :::::::::::::::Rv378T7.seq:::::::::::::::
    CCNGAACAGAAGCGGNGGTTCCTACCGCGGTGTGCGGCCGGCGCGATATCGGGCCTTTTTACTACCGAACCCGATGTGGGCTCCGATCCGGCGCCCATGGCATCGA
    CGGCGACGCCGATCGATGACCGCCAGGCTTACCACCTT
    Clone Rv379
    :::::::::::::::Rv379SP6.seq:::::::::::::::
    CTCAAGCTTGCGCGACTCGACAAGCATTCTTGACAGTTGTTTTGGCTCGGCATGGTTAGCCAAGGTTCTGCGGTCCCACCAGATCATCTTGGTCCGGTAGCGCTCG
    TCCGGGTATGCTGCGCCGGGATTCTCGCTGCTATTACTCCCCCCCGAAGAACGCCACCGGTCCAGCGC
    :::::::::::::::Rv379T7.seq:::::::::::::::
    GCNAGGCGGTATAGCTTCCCGTCGTACCGGCGACCGCCAGCCGAGAAGCTCGTTTTCCCAGTGTTGCTGGGGATTCTCACGCTGCTGCTGAGTGCGTGCCAGACCG
    CTTCCGCTTCGGGTTACAACGAGCCGCGGGGCTACGATCGTGCGACGCTGAAGTTGGTGTTCTCCATGGACTTGGGGATGT
    Clone Rv37
    :::::::::::::::Rv37SP7.seq:::::::::::::::
    GTGTGGAACCGTGAGCCGGATAACAATTTCACACAGGAAACAGCTNTGACCTTGATTACGCCAGCTATTTAGGTGAGGCTATATTAATACTCAAGATTGCGGTCGA
    GCACATCGGCCCAAGAACCGCCGAAGGCACGGCGGAACGCCTGCGGCACATGGGGCGACGACCAGCGGGTCGGACTTCTGGGCTGTCCAGCCGGATCGCGCCGTCG
    CGA
    :::::::::::::::Rv37T7.seq:::::::::::::::
    CACTGTCAGACATATGCGCCGCTCCTCCTCATCGCTGCGCTCGGCATCGTCGCCGGCGGTCATGGCGTCACCCTACCCAAGCCGAAACGCGAAACGAGAACGTGTT
    CCATTATTAGGGTGTGAGCACCAATACCAGATGCTCACCAGGAACTCACGCAGCACCGGGACGGATGTCAGCCACCACGCCCATCTGGGGTGGTAGCGGGGGAAAT
    ACGGCTAACGCGGCTCCGGTGCCGGCAGCCCAGCGCAGCCCTCGGCGGCGGACACGGCAAACAACGACGACCCATAGTTGTTCTTTGCCGGATGGCCCCGTGTTGC
    GGACATATCGGGCGGCGGCGCGGGCGCCGCCGAGGTAGTGGCTGAGGCCCATCTCGTGCCCCGCCGAATGGCCCCAGCCAAACCGTGTA
    Clone Rv381
    :::::::::::::::Rv381SP6.seq:::::::::::::::
    CTCAAGCTTTTACGGTGATCGCGCATCACCTGGTTCATGAACTGGAAGCAGCGCAGCGCTTCCTTTTCGGCCGCAACATGAGCCANCCTCTCGTCGGCGGTCGGGT
    GCAGGTGCTCGGGCAGCTCGGCCGCGACAGCCGCCTGACCCTGAAACCAGCTTCCATATCCCGCGACNAACGAC
    :::::::::::::::Rv381T7.seq:::::::::::::::
    CTCAGAAGCCGCTAGCTGGTAGAGTCGCTGACCGGTGCACGTGGCGNCAATGTGCGCTGCCGGTTCGCG
    Clone Rv382
    :::::::::::::::Rv382SP6.seq:::::::::::::::
    CTCAAGCTTGCGCTCATCAAGCGCGAACAGCAGGGCGGTCGGCTGGTCGCCATGACGGGTGACGGGACCAATGACGCACCCCGCGCTCGCGCAAGCCGATGTCGGG
    TGGCNATNAATACCGGCACCCAGGCGGCCCGGGAAGCCGGCAACATGGTCNATCTCCACTCC
    :::::::::::::::Rv382T7.seq:::::::::::::::
    ACTTCTATTTCGACTGGTGTGCTGTGGCGCGATCCGACTGCCGGCGTGGTCAAGGCCGGCCAGTTGTGGGATNCCACAGGCAC
    Clone Rv383
    :::::::::::::::Rv383SP6.seq:::::::::::::::
    GCTTGTCGTATTCCGTGGCACTGTCAGACATATGCGCCGCTCCTCCTCATCGCTGCGCTCGGCATCGTCGCCGGCGGTCATGGCGTCACCCTACCCAAGCCGAACG
    CGAAACGAGAACGTGTTCCATTATTAGGGTGTGAGCACCAATACCGATTGCTCACCAGGAACTCAC
    :::::::::::::::Rv383T7.seq:::::::::::::::
    CGATATTCGTCGGCCGCGTTGTCTCGACTGGGTCGCGT
    Clone Rv384
    :::::::::::::::Rv384SP6.seq:::::::::::::::
    GACCTCGGCCACCAAGCCGGACGCGACCGTCGAGGTGGCGATCCGGCTTGGCGTCGACCCGCGTAAGGCAGACCACATGGTCCGCGGCACGGCCANCCTGCCACAC
    GGCACTGGTAAGACTGCCCGCGTCGCGGCN
    :::::::::::::::RV384T7.seq:::::::::::::::
    CCGGAAGTCTAGGGGACGACCTACTCAGCGCAAAATGTCGCTAATGTGAGTCCGCCCCACCAGGGCAGATCAACCCATGTCGATGATGACCTACCCGGATAGGGGA
    TTGGCGGT
    Clone Rv385
    :::::::::::::::Rv385SP6.seq:::::::::::::::
    AGCTTCAGTTCCTCCACGACGCGTTCCCAAATGATTTCCCGATCCCACAATCTCGGTTCAGATACAGGTCGCCATAGCCCTTACTTCGGNAACGCGTGGCCCGATT
    GGCCCTGCCGCTG
    :::::::::::::::Rv385T7.seq:::::::::::::::
    CCGCCTACGGGTCGAACATGCATCCCGAGCCGATGCTCGAGCCGCGCACCCCACTCGCCGATGGCCGGAACCGGCTGGTTACCCGGGTGCCGGCTGACC
    Clone Rv386
    :::::::::::::::Rv386SP6.seq:::::::::::::::
    GCGGCTGGTTACGACTCCCTGTTTGTGATGGACCACTTCTACCAACTGCCCATGTTGGGACGCCCCGACCAGCCGATGCTGGAGGCCCTACACGGCCTTGGTGCGC
    TGGCCACGGCGACCGAGCGGCTGCAACTGGGCGCNTTGGTNACCGGCAATACCTACCGCAGCCCGACCTGCTGGCAAAGATCATCACCACGCTCGACGTGGGTTAG
    CGCCGGTCGAGCGATCCTCGGCATTGGAGCCGGTTGGTTTGAGCTGGAACACCGCCAGCTCGGCTTCGAGTTCGGCACTTTCAGTGACCGGTTCAN
    :::::::::::::::Rv386T7.seq:::::::::::::::
    GCCTTTCCGCACAATCTGTACCCCAGGACCNTCTAAAAATCGAATACGACGGCGTCGCCGACTTTGCGGGGTACCCGCTCAACTTTGTGTCGACCCTCAAGGCCCA
    TTGCCGGCACCTACTACGTGCACTCCAACTACTTCATCCTGACGCCGGAACAAATTGACGCAGCGGTTCCGCTGACCANTNNTGTCGGTCCCACGATGACCCAGTA
    CTACATCATTCGCACGGAGAACCTGCCGCTGCTAGAGCCCACTGCGATCGGTGCCGATCGTGGGGAACCCACTGGCGAACCTGGTCAACCAAACTTGAAGGTGATT
    GTTAACCTGG
    Clone Rv387
    :::::::::::::::Rv387T7.seq:::::::::::::::
    GCAGCCAACAAGATGCATCGGGATCATACGCCGTCAACTACCCGGCCAACGGTGATTTCTTGGCGGCCGCCCCAC
    Clone Rv388
    :::::::::::::::Rv388SP6.seq:::::::::::::::
    CTCAAGCTTGCCAAAGAGACCTCGTCCACCAAGCNGGACGCGACCGTCNAGGTGGCGATCCGGCTTGGCGTCCACCCGCGTAAGGCANACCANATGGTCGGCGGCA
    CGGTCAACCTGCCACACGGCACTGGTAANACTGCCCGCGTCGCGGTATTCGCGGTTGGTGAAAAGGCCGATGCTGCCGTTGCCGCGGGGGGGGATGTTGTCGGGAG
    TGACAATCTGATCGANAGGATTCAGGCGGCTGGCTGGAATTCGATGCCGCGATCGCGACACCGGGATCAGATGGCCAAAGTCGGGTCNCATCGCTCGGGTGCTGGT
    C
    :::::::::::::::Rv388T7.seq:::::::::::::::
    CCACGGCGTGGATCAAGGTACCGGCCGGATGTTGCGCAATGGCAGGTTGTTGCCCGGCTTGATGTCGGCGTTAGCGCCGGATTCCACCACATCCCCCTTGCGAAAG
    TCCGTTGGGTGCAATGATGTAGCGCTTCTCCCCATCGAGATAGTGGAGCAAACGCAATCCGTGCGGTACGGTTCGGGTCNTACTCGATGTGCGCGACCTGGCGTTG
    ACACCATCTTTGTCATTGCGGCGAAAGTCGATCATCCGGTAAGCGCGCTTATGACCGCCCGCCTTTGTGCCGGGTGGTAATCCGGCCATGCGCGTTGCGTC
    Clone Rv389
    :::::::::::::::Rv389SP6.seq:::::::::::::::
    GGCGGCTGCGTCGGCGAGATGATCGCCCGGTGCCACCCCGATCCGTGCCTCGGTCAGCGCCAACGTGCTTCCGGTCCGGCGACCACCATGTCGCATGCGCCCCGAC
    :::::::::::::::Rv389T7.seq:::::::::::::::
    GCAATCGCCTTGGCGGTCGCCGGTTGTCACCGGTGATCATCNCGGNGCGGATGCTCATNCGGCGCATTTCGTCNAATCGTTCCGTATGCCCACCTTGACCCGATGT
    CTTCATATGGACCACGCCGATGGCCCNCGCGCTNCTG
    Clone Rv38
    :::::::::::::::Rv38SP6.seq:::::::::::::::
    CCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACACTATAGAATACTCA
    AGCTTCCACATCGGTATGCCAAAGCATTGCGCCGCTATCGATTTCGCGCTGGCATCGCCAAGGTGGACTTCTTGCTCAGCGACGAGATCCCGTGGTCGGATCCGCG
    GCTGCGGCGGGCTGCGACCCTGCATCTCGGCGGCACCCGTGACCAGATGGCGCGCGCCGAGGCAGACGTCGCGGCGGGACGCCACGCCGACTGGCCGATGGTGCTG
    GCCGCGTGTCCGCACGTCGCCGACCCCGGCCGCATCGACGAAACCGGCCGCCGTCCGTTCTGGACCTATGCCCACGTGCCGTCGGGGTCCACGCTCGACGCGACCG
    AGACCGT
    :::::::::::::::Rv387T7.seq:::::::::::::::
    CGCGTCCACCGCAGCGTGAGATTGGTGGCGCCATTCGTCGTGGTGTAGCTGCTGTTGGCGGGCGTCGCCGTATTGTGCGGGCCAGCCTTGTGCGGGGGCCGTTCTA
    CCCACGAGTCGGCACTTCCGCAACCGCCCAGCTCGACCCGCGATTACGGCGGCCGCAACGGCCGCCGGAAGGCGTCTCGCAAGCGCCTTATCCTTTCGCAGGTTCC
    AGATCCTTCCGCTACGTGGTCGCTCATCGGCGGCCCGGCCGAATGAGTACAGGTGAGGGTAACCGCTACAAATGAAGTTGGTCAGTGCTGGCCCCAACTGTGTAAT
    GGTTGCCCGGCTCGGGTCACCACGTACATTCTGGCAAGGCGGGCGAGATTCGGTTCCTCGCGTCCTTGGCCGGTGGCGGTTCCCGGTTGTCCGTGGGCGTGTCGTG
    TACGTGGTGTAAGTGTCGTGAACTCCTCAGTTTGGGCT
    Clone Rv390
    :::::::::::::::Rv390SP6.seq:::::::::::::::
    CTCAAGCTTGCGCTGGATCTGGCGGCTGAGCCTGTTCTTGGCAACATGCCGAGGGATCGCCTTTTCCACCACGCGGTCGGGGTGCGTTGCATTAGCTCACCCCGAT
    GGTGCGCTTGTGCAGGCCGCCGGGATACCCCGAGTGCCGGTAAACCATCTTGTGCTGCAGTTTGTCGCCGCTGATGGCGACCTTGTCGGCGTTGATCACANATGAC
    AAGTCACCGCCATCGACATTGGGGGCGAACGTCGGCTTGTGCTGCCGCGCAGCAGGTTGCCGGCCCGCGACGGCAGGGGGGGAANCACCACGTC
    :::::::::::::::Rv390T7.seq:::::::::::::::
    TTTGGGATGCGGCAAAAAGGCGAAGCNCCGCGTGGCCACAACGCCGGGAGGGACAATCTCGGGCGGCTAGGGCTTCTCGCGGGAAGGCCCCGAACGTACGGCGTTC
    AACACGTCGCGTCGCCCTCCGACCGCGAACATTCGGGGATGGCAGCAACCTGGTAGCACCCTGGCCGGGCGATGATCTGCAGCGTCGCCCGCGGGTAGTCGCCCCC
    GGCGGCTACAGTCTGAAACGCGATGACCATCGATGTGTGGATGCAGCATCCGACGCAACGGTTCCTACACGGCGATATGTTCGCCTCGCTGCGCCGGTGGACCGGC
    TGGGTCTATCCCGGA
    Clone Rv391
    :::::::::::::::Rv391SP6.seq:::::::::::::::
    CTCAAGCTTCGTCATAAGACCATGGTGCGCTTTCTTTCACCCGTCCANAGTCGGGGGCATCCGCACCGGCTCGCATCGCATCATCGCTCCCACGAGCGGGGCTCAT
    CAGCTTGGGCCATTTCAATGTACTTGATACCCCGCGCTGCGGGTAGGCCACTGCNACAATTCAAACACGGTGTCACACGGTGAATANTGTCNANATGGGCTCTGAT
    CAACCGTCNCAAACCCGGTTC
    :::::::::::::::Rv391T7.seq:::::::::::::::
    GAATTCTGCGTGCACCGCTATGGGTTGCAGCAGCGGCTGGCGCCGCACACCCCACTGGCCCGGGTGTTTCGCCCCGAACCCGGATCATGGTGAGCCGAAAAGGCGA
    TTCGCCTGTTCGATGCTGGGATTCGCCACCGCGAGGCCATCGACCGATTACTCGCCACCGGGGTGCGAGAGGTGCCGCATGCCCCTCCCGTCGACGTCTCCGACGA
    TCCATCCGGCTTCCGCCGTCGGGTGGCGGTAGCCGTCGATGAAATCGCTGCCGGCCGCTACCACAAGGTGATTCTGTCCCGTTGTGTCGAAGTGCCTTTCCCGATC
    GACTTTCCGTTGACCTACCGGCTGGGGCGTCTGCACAACACCCCGGTGAGGTCGTTTTGTTGCAGTTGGGCGGAATCCGTGCTCTGGGTTACACCCCCCCAACTCG
    TCNCGGCGGTGCGCGC
    Clone Rv392
    :::::::::::::::Rv392SP6.seq:::::::::::::::
    GCAGTTGGGAATCGCTCTGCAGCAAACCANTATTCTGCGCGACGTTCGAGAGGACTNTTTGAATGGACGGATCTACCTGCGCGCCGACGAGCTGGACCGATTAGGC
    GTACNCCTCCGCCTGGACGACTCCGGGGCACTCGATGACCCCGACGGACGGCTCGCCGGCACTGCTGCGGTTCANTGCCNACCGCGCCCGANACTGGTATTCCTGG
    GACTGCGGCTGATTCCACACCTCGACCGCCGCAGCGCTGCCTGCTGTGCGGCCATGTCTGGCATCTACCGCCGTCAGCTCGCCTGATCAGACCATCGCCCCCCCCT
    CGTCTACCATCGGCGAATCTCTCTGTTCGGGACTGAANAANAGCCCAAGTGGCGGCGGCAGCACTGGNACTCTTCGGTAACCTGCNGACCGGATTGGACCGCTACC
    G
    :::::::::::::::Rv392T7.seq:::::::::::::::
    TTGATCTGGACGTCTGAGCGGTGATCGGNCCGAACCTGAATTGTCCGGTAATGCCCAGCGCAGAAAGCANGGTGGTGGCCGGGGCGGTGAANCCGGCGTCGGCGCG
    CACCGTCGAAGTCGATGTGGATTGCCGGAATGGGGATGTCCGGCACGGCGAAGCCGTAGTTCGCTTGTCCCGTGAGGCCCANGTGGATGGGGGGAAGGATCGTGGT
    GTCCGGGATGATAATGGGGCCCGATGCCGCCGGTTGAAGTCCAGTGGATCGGGAATTCGGGAATCGTGATGCCGACGTTCAGGCCGAACAGGCCCTCCAAGTGCCT
    CGCCACNAGATGCCGTTGCTGAAGTTGCCCGACATGAGGCGCCGGTGTCCACATTGGCCCGAATTGGCGACGCCGGTGTTGGC
    Clone Rv393
    :::::::::::::::Rv393SP6.seq:::::::::::::::
    CACGTAGGCGCCGTCCATAAATNACTCCGCCGCGCTTCGCACNTCCTCGTANCGATCCTTGGCGAGCAGGTCANCCGGCGCTGCCCGTCNAGGACCCCCTTTTTTG
    GCGTGCAGCCACTGGCCGACACCTCGGGGGTAAGCGAATCCGAGAGCAGGAGGACNAGGTCACGAANCTGCGCCAGCCCCGGTCGTACCGTCAGGGGGGTGTCCGC
    CGGTCCGCCACCCCGCGTACCGCCCGATCGGACACCTGTATGACCGCGGCGACNTCGACCTGGGTGACGCCGAAGGGTTTCAGGGCATCNACNATCTCGGCTGGTC
    GACCGCCCGGTCCAGGGTGACCGCCATCGTGGTTCCTCCGCAACTTCCGGTTCTACTACCGTAAACGCTACCG
    :::::::::::::::Rv393T7.seq:::::::::::::::
    CGGGGAACGGTCCTCGCACACCTGGTTCGTGTTGCGGGAATTACTCGGACANCAAAACGTCAAGAACTACGACGGCAGTNGGACAGAANACGGCTCCCTGGTGGGC
    GCCCCGATCGAGTTGGAAGCTGATATGTGCTCTGGACCCAAGCAAGGACTGACATTGCCGGCCAGCGTCGACCTGGAAAAAAGAAACGGTGATCACCGGCCGCGTA
    GTGGACGGTGACGGCCAGGCCGTGGGCGGCGCGTTTCGTGCGGCTGCTGGGACNCCTCCGACGAGTTCACCGCCGGGAAGGTCGTCGCGTCGGCCACGGGCGAATT
    TCCGGTTCTTTCGCCGCGCCCCCGGATCCTGGGACCGCNGGCGCGCGCTTGTT
    Clone Rv396
    :::::::::::::::Rv396SP6.seq:::::::::::::::
    CTCAAGCTTTGTCCGACAAGCGTTCCCGGGCGGTCAGCAAGCGAACGTCGGTTGGCCCACTGCGGGTCGATATTGCCGCCAGGGA
    :::::::::::::::Rv396T7.seq:::::::::::::::
    CGTCAGCACGGCGACGTCGCGNTACGCCGAGCAGTTACACAATCGCTCTGCAGCAAACCAATATTCTGCGCGCACGTTCGAGAGGACTTCTTGATTGGACTG
    Clone Rv39
    :::::::::::::::Rv39SP6.seq:::::::::::::::
    CTGCATCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACACTATAGAA
    TACTCAAGCTTCGCGCAGCGGCGGGTTGACCCGGTTCACGCCGTCATAGCTGGCCAATCTGGCCATCGTCGATCANCATGTGGTGGGGGGTGACCTCGCGGTGATC
    GAAATACCCTGGTCCTTATCCCATTTCAGGATTTCGACGGTGCCCGCGGCCGACGCGTGACAGTGTGCACCCGGGCGCCGGCGTCACGGGCCAGCAAGGCGGTCGC
    GGGACGATCGATTCCTCGGCGGCCCGGGGCCATCCCGGCCAGCCCCAGCCGCGCCGCCATGGGTCCCTCGTGCGCGCGACGGCGCCGACCGTCAGCCGCGCTCCTC
    GGCGTGCTGGGCGATCAGCACGCCCAAACCGGTG
    :::::::::::::::Rv39T7.seq:::::::::::::::
    CCGACGCGCACTACGTGCTGGTGTCCACCCGCGACCCGCACCGGGCACGAGCTACGCAGCTACCGCATCGTCGATGGCGCTGTCACCGAGGAACCTGTCAATGTGT
    CGAGCAGTACTGAACCGTTCCGAGAAAGGCCAGCATGAACGTCACCGTATCCATTCCGACCATCCTGCGGCCCCCACACCGGCGGCCAGAAGAGTGTCTGGCCAGC
    GGCGATACCTTGGGTGCCGTCATCAGCGACCTGGAGGCCAGCTATTCGGGCATTTCCGAGCGCCTGATGGACCCGTCTTCCCCAGGTAAGTTGCACCGCTTCGTGA
    ACATCTACGTCAACGACGAAGACGTGCGGTTCTCCGGCGGCTTGGCCACCGCGATCGCTGACGGTGACTCGGTCACCATCCTCCCCCGCCGTGCCGGTGGGTGAGC
    GGACACATGACACGATACGACTCACTGTTGCATGCCTTG
    Clone Rv3
    :::::::::::::::Rv3SP6.seq:::::::::::::::
    TGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGCCATCGATTACGCCAAGCTATTTAGGTGACACTATAGAAT
    ACTCAAGCTTGCCGGGAGGGTGCATGGCCGACTCGGATTTACCCACCAAGGGGCGCCAACGCGGTGTCCGCGCCGTCGAGCTGAACGTTGCTGCCCGCCTGGAGAA
    CCTGGCGCTGCTGCGCACCCTGGTCGGCGCCATCGGCACCTTCGAGGACCTGGATTTCGACGCCGTGGCCGACCTGAGGTTGGCGGTGGACGANGTGTGCACCCGG
    TTGATTCGCTCGGCCTTGCCGGATGCCACCCTGCGCCTGGTGGTCGATCCGCGAAAAGACGAAGTTGTGGTGGAGGCTTCTGCTGCCTGCGACACCCACGACGTGG
    TGGCACGGGCAGCTTTAGCTGGCATTCCT
    :::::::::::::::Rv37T.seq:::::::::::::::
    GGAAACACCGNCGCCGTCGTGGCCACCAACACCGCGACCAGCACCGTGACCCGGACCGGGGTGCCGCGCGAACCGGTCTTGGCCAATTGCCGCGGCACCAAGCCGT
    CGCGCGCCATGGCGAACAGCACGCGGCATTGCCCGAGCATCAACACCATCACCACCGTGGTAAGCCCGGCCAGCGCGCCGACGGAGATGATGCCGCTGGCCCAGTA
    CACCCCGTTGGCCTGGAACGCGGTGGCCAGATTTGCCGGCCCGCGGCCCGGTACGGTCCGCAGTTGGGTGTATGGAACCATGCCCGACAGCACCACCGATACCGCG
    ACGTAGAGAAGGGTCACGACCCCAGCGACGCGAGAATCCCTCGAGGGACGTCTCGTTGAGGACGCTTGGTCTCCTCGGCCATGGTGGCCACGATGTCAAACCCCGA
    TAAACGCGAAGAACACGATCGATGCCCGGCCAGCACGCCGTA
    Clone Rv40
    :::::::::::::::Rv40SP6.seq:::::::::::::::
    CCTGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACNCTATAGA
    ATACTCAAGCTTGTCCTCGGGCGTTGGCCTCGGCCAAGAAATCGTCGACGCCGGCCTCCTGTGCAATCGCCTTGGCGGTCGCCGGGTTGTCACGGTGATCATCACG
    GTGCGGATGCTCATTCGGCGCATTTCGTCGAAGCGTTCCCGTATGCCCACCTTGACGATGTCCTTCAGATGGACGACGCCCGATGGCCCCGCGCTGCTGTTATCGG
    TCCATTCCGCAACGACTAGGGGTGTCCCCCGCCGGAGCTGATGCCGTCGACAATGGCACCCAACCTCCTCAGTGGGGTGGCCACCGTGATCGCAAAACCACTTCAT
    CACCGCAGCCGCGGCACCTTTGCGGATCCGAACGGATGCGCTC
    :::::::::::::::Rv40T7.seq:::::::::::::::
    TTCGTTCGATGGCGCCGCCCCGGCTACGGTTTGACCTGTGGGTGTCGAATTGGGGTCAAATTCCGAGGTCGGCGCGTAAGAGTGGTCATCCTGCAGGGGGGCCGGG
    GCCGAACTGCGCCGGCTCACACCGCGCAACACCGACCAGCTGCTGTTCGACGGCCTGCCCTGGGTATCCCGCGCGCATCAGGAGCACGACGAATTCGCCGAGCTGC
    TGGCTTCCCGCGGTGCGGAAGTGCTGTTGCTGTCGGACCTGTTGACTGAGGCACTACATCACAGCGGGGCCGCCCGCATGCAGGGGATCGCCGCTGCCGTCGACGC
    ACCGCGCTGGGACTGCCGCTGGCGCAAGAACTTTCGGCCCTACCTGCGTATCTCGACCCAAGCANGTTGGCGCATGTGCTGACGCCGGCATGACTTCAACGAACTC
    CCNTCCGACACGCCGAACGAAGTGTCGTTGGTGTTGCGTATGC
    Clone Rv412
    :::::::::::::::Rv412SP6.seq:::::::::::::::
    GCGGCGAGTGTGGTGGGTGCCGAACACGAATCCAACGACGCACTGGCGGAGAGATACCACTTGCTGTACTGGAAGCAGTGCTGATGATCTCCCGTGGAATGTGCCC
    TCGCCGCCGTCTATCGAAAACAGTGGCATGCTGCG
    :::::::::::::::Rv412T7.seq:::::::::::::::
    CAACCGCGCTCGGCGCGTCTGGGCCTTCCGCCGGCTCCGCCGACAATTCTATCTCTGGATCAGCGGGGCTCTCCGGGCCGGCCTCCGCGAACTCAACAGGCCGCGC
    CTTCCGGCCGAAACATTCCCTAGCCATATATGATCGCACCTCGATACACGATCTGGCGGCAACACCGCAAAGCGTCCGACGGGCCCCAACCTCCGCAATTCAGTAT
    CCGGG
    Clone Rv413
    :::::::::::::::Rv413SP6.seq:::::::::::::::
    GAAGGTCGGCGAAGGTGTGGCTGGNTGCCGATCACGAATCCAATGATGCAGTGGTCGGAAGATATTAGCCACTTGCTGTTCTGGAGACAGGTGCTGATGATCTCCC
    GTGGAATCTCCCTCGACTCCGTCTATCGAAATCGTGAACA
    :::::::::::::::Rv413T7.seq:::::::::::::::
    TCCTGCGCTCTGGGCCATTCTCGGGTCTGCCGACAATTCTATCTCTGGATCTGTGGGGCTCTCTTGGCCGGCCTCNGCGATCTCTCTTCANGGCGCGCCTTCCCGG
    AAACATTCCCTATCCATATATGATCGCACCTCTATACACCGTTGGCGGCAACACCGCAAAGTGTCTGTCG
    Clone Rv414
    :::::::::::::::Rv414SP6.seq:::::::::::::::
    AGCTTTACGCTGGCGTATCAGCGTTGGGGCCGCTGCCATTTCGGTCGCCCAACGCGTTGCCAGCTCCCTGCGCTGTCAGGGGCTTGCGGCAAACTGGCCACCCCCA
    ACAAACTTGGCTGAGCTTGATC
    :::::::::::::::Rv414T7.seq:::::::::::::::
    CTCTATCTGGCGTCACATTCGCAATCTTTAGATTGCAGATATCGATAAAATCACCCGCGCGACAAGACCGCCCATGTCATCCTTCGATGTTATTTCGCCGGCCTGG
    GGAAAGCGCAACGACGTTGCCTACACGTTCCGCCGT
    Clone Rv415
    :::::::::::::::Rv415SP6.seq:::::::::::::::
    AGCTTTNCCTTGCATCTGCACCCCGATCCACGTCAGCCACGTCGGCGTTCTCCACCAAGAAGTTGCGGGCATTCTCCTTGCCCTGCCGAGCTGCTCGCCCTCCGTA
    GGTGAACCAGGCACCCGACTTGCGGATGAGGCCCTGATCCACACCCATGTCGATCAGCGAGCCCTCCCTGCTGATCCCTGCCGTAGAGGATGTCGAACTCGGCCCC
    TGCTTGAAGGGGGGCGAACAGTTGTGCACGACAACCCTTCGGCGACGAGGGTGTGCAGTTCCTCGACCTCGAGGTCGAACGTTCGTGCCCCGCCGTTGGCAGCCCA
    CTTCTCGGATCACGGAATAGCGGANTTCTTCCGCCAGCATGTCGTGCAGGAATTTGTCATCCAGGGCATCCGCGAGCGCCTGCACGCCG
    :::::::::::::::Rv415T7.seq:::::::::::::::
    ACTGTCNAGGGAATGCTTCGCAGCATCTACCTGCAGTCGCTTGTGCATAAGCGGACGGCCCNAACCTGTTCGTGTTCCGGGGACACCAGACGGGGGGAGCAGCAGT
    ACGGCGAAAGGTTTGAGCGGAAAGGAGTTGCGCAAATCGGGCGCCCCCAACACCCGTCCGCAAGACGCGGTCAACGAACCTGTTCAGGGATCAGGGTCAGGCGACT
    ACCTGCACTGAGAACAAGCGATCTGCTGATCTGCCAGAAGATGGACATGAATGTCCACGGCAAGCCTGATGGCCTGCCGCTCTTCCGGGAATGTTTGGC
    Clone Rv416
    :::::::::::::::Rv416SP6.seq:::::::::::::::
    TGAATTATGATCCCGACACAACTGCATCANTTTAGCCGCGTCGNGATGCTATCCGCCGACGGTTTGGANCNGGTCCGTGTCGTTCGTGTTGATCTCACCCGAAGTT
    CTGTCCGCCGCCGCCGGGGATCTAGCGAACGTGGGATCGACAATCAGCGCCGCCAACAAGGCGGCAGCGGCTGCGACCACGCAGGTGCTGGCGGGGGGGGCGGATN
    AGGTGTCAGCGCGCATCGCGGCGCTGTTTGGTATGTACGGCCTGNAATATCCGGCGATCAGTGCGCAAGTTGCCGCGTATCACCANCAGTGCCGTGCAG
    :::::::::::::::Rv416T7.seq:::::::::::::::
    AACGGGGACCNCAAGAAACCATTCAANAACGAGGGGTCGTCACCAACGTCGAAACCGACGGTTGCCAGCCGGCCCACGATATTGCGTGCTCGAGGGTCCGCTGTAC
    CCTCACCGAACGTGAGTCCCACACCGCGGAGGCGGGCGACTCTGGCGTCGTTAGCAGCCCGAGCTCAAGGTGTCCCGCACCACTGTCGGAATGCTTTTAACCGACC
    GGATCAGCTCTCCGCCGATCTACGTGAACGAGTGCTTGCCACGGCCAAGCGACTGGGCTATGCCGGACCGGATCGGTGGCGCGATCGTTGCGGACCCCGCCCCGCC
    GGTGCGGT
    Clone Rv417
    :::::::::::::::Rv417SP6.seq:::::::::::::::
    AGCTTTGGAGCCNCNCCGANCCNCCGGTACGCCCCGCCACCGCCGTACCCGGCACCCGACCCCTTTGAGCCGTTCGCCGTGGCCGCGGTGGANCTGGCGACGAGGG
    GACGATCGTGCTGGCAAAGTGGTCGATGGCACGCTGGCCGCCGATCTGAAGGTCGGCATGGAGATGGAGCTGACGACCATGCCGCTGTTCGCCGACNACGACGGGG
    TGTGCAGCGCATCGTCTACGCGTGGCGGATCCCATCGCGCGCCGGCGACNATGCANAGCGCANCGATGCTGAGGAGCGGGGGGATGAGGATGAGCCCCCCGGGCAC
    CCGTTTACMTCCTGGGTGCCGGTATGCACCCGTGGGGAAATGGGTAATGACTTC
    :::::::::::::::Rv417T7.seq:::::::::::::::
    TTCTCNCATCGTTCGTACTNNGATGGACGCTGCTGCCCCGAGGCGATCCTGGCCAACCGGCCTCTCGCCCCCCCGACCTTCGGGGGGAACCTGAACTTCTTTTTTC
    CGATGGGCGCTTGGGCCAAACGTACCGGGGCTATCTTCATTCGGCGTCAGACGAAGATATTCCCGTCTACCGCTTCGTATTACGTGCTTACGCCCCCCCAGCTGGT
    GCAAAACCATGTCAACCTCACCTGGTCGATCGAAGGGGTCGGACCAGAACGGGCAAGCTACGGCCACCGGTGTTCGGGATCCTGCGTTACATCACCGATGCGCGTC
    GACGAAATCGACGGTCCCGAAGTGTATTGGTCCCCACCTCGATCGTGTACGAACAGCTGCACGAAGTGGAAGCCATGACCACCGAAGCCTATGGCCGGTGAA
    Clone Rv418
    :::::::::::::::Rv418SP6.seq:::::::::::::::
    TTCTTCCGGGTACCGCTGATCGGCGGCACCATCACGCACCCCGGTGCAGGGCGAGGCGGCCCGCCGGTGTGGTGTTGCTACGGCCGGCCAGCCCGTACCGGTGTGA
    TCGCCGGTGGTGCGGCCCGCGCGGTGCTGGAATGTGCGGGGTGCACGACATCTTGGCCAAGTCGCTGGGCAGTGACAACGCGATCAATGTGGTGCACGCCACCCGT
    GGCCGCGCTCAAGCTGCTGCACCGTCCGGAGGAGGTGGCCGGCGCGCCGCGGTTTGCCAATAGAAGACGTCCCCCCCGGCCGGGATGCTG
    :::::::::::::::Rv418T7.seq:::::::::::::::
    GTCGAAAGTGACCATCTCTACCTTGAGTGCCATACCGCCCCGACCCTATGCCTCGGATAGCTCGGCGGAAAGAAACGCTTGCAGTGCCGCCCGAATAGGCCTACGT
    CGTGAGCGCCCATCAACTCTCGCGCGGAGTGCATCGCCAGCTGGGCGGCGCCGACGTCGACCGTGGGATTCCGGTGCGCGCCGCGGCCAACGGCCCCGATCGTCGA
    CCCGCACCGGCAGATCGGCGCGATGTTCGTAACGCTGCATAGGCACTCCCGCGCGCTGGCAGGCCAGTTGCGAAACGCCCCCCGCGGTGCCTTCCGTCGGTTGGCT
    TTACCGCAAATTTGGGGTTGCCCCT
    Clone Rv419
    :::::::::::::::Rv419SP6.seq:::::::::::::::
    AAAGCCACGGAAACGATTGCCTACTGCCGAATCGGGGAACGGTCCTCGCACACCTGGTTCGTGTTGCGGGAATTACTCGGACACCAAAACGTCAAGAACTACGACG
    GCAGTTGGACAGAATACGGCTCCCTGGTGGGCGCCCCGATCGAGTTGGGAAACTGATATGTGCTCTGGACCCAAGCAAGGACTGACATTGCCGGCCAGCGTCTACC
    TGGAAAA
    :::::::::::::::Rv419T7.seq:::::::::::::::
    TTTCGCCACCGCNAGGTCGTGCGCGTTCCAGAAAAGCGTGGTTCGGCCGGGCGCGAGGATTGGACGGTCCAANCTGACCAGCCGGTCCGGCCACCCGTTAGGCAGA
    TCGCGGTGTGTATATGTTCGGCCCTCGGCATAAACGCCATTGCTGCGGTGAAAATCGGACATCTCGCCGATTGCCAGTCTACATGATCCGCCTTGTCCCCCCCCCG
    GTCGTTGACAAACGCGATGTCNGCCTCCTGGGAAGCGGTGGC
    Clone Rv41
    :::::::::::::::Rv41SP6.seq:::::::::::::::
    TCGCCAAGTGGATTCGTGCTCACCNACGAGATCCGTGGTCGGATCCGCNGCTGCGCGGGCTGCGACCCTGCATCTCGGCGGCACCCGTGACCAAATGGCCGCGCCG
    CGAAGCAGACGTCTCGGCGGGAGCGGACGCCGACTGGGCGATGGTGCTGGCCGCGTGTCCCNGTCNCCCGACCCCCCCCATCNACCAAAACCGGGGGGGGGTCCCG
    TTCTGGACCTATCCCACGTGCCNTCGGGGTCCACGCTCGACGCGACCGANAACGTAACCAGCGTCCTCGANCGGTCGCCCCCCCCCTTCCGTGACATGCTGGTGGC
    GGCCGCGCCGT
    :::::::::::::::Rv41T7.seq:::::::::::::::
    GTACCGTCACCATGATCGCCCCCCATCGGCATCGGTGAGCTGATAGATCCCAGCCGGTTTCGCCAACCCCCGAGCGATCTTGGCGGCTGCTNGTNGTCGNTGANAC
    NTAGCCACCAACAGAGCCCGGTGTGCGACAAGNGACTGATCGGATCTCTCCGGACACNTTCGAGGGGGGTCNTCAGGAGNCCGGGCGCCACCCCGAGGTAACCTCC
    GCCCAGCCTCACACCGCGACCGGGTATCNCAAGTCGCGCAATANCCCACCACCTCCTCGGACCCCCACGTTGTATGCGGCTGGT
    Clone Rv42
    :::::::::::::::Rv42SP6.seq:::::::::::::::
    ATACTCAAGCTTAGACCTCACTGATGTGGCGGGACGCGGGAGATAACCGCGGTTCGAGCCGTTCAACAGTGGTGTTCCCACACCAGTTGTTGCCTTTGCCCGAAGT
    AAAGCGATTCGATTTGCTCGAAAAGAGGGCTGGCTGCTCGTGAGGGACATCCATGGCCGATACCTCAGCGATCTCAACGGTCAAGCGACTGCATGTTTGGCGCAAG
    GTATCGCTAAGCATAGGTTCGTGACGGATTTGACAGCAAGAGCTTTCCAAAGATTGCTGTCCACATANTGATCGCATCTCTACACCTCTTCGCCGGTCGCTGTCAA
    GAGCCATTCGAATCAGTTATCTCGCTCGTGCTTGGAAAAAATTTCCAGCCTGCGTTGGACAAACCGCGTCGCCAAAGCGGT
    :::::::::::::::Rv42T7.seq:::::::::::::::
    AGCTTCCCGAGAAACAGTGCATTCCCAAGCAGCCCGTTGTCACGCCGATGAGTGAAGAGTGCACGCAATCGCCGGAATCCGGCAAAGCCCCTGCACAAGCGAAATC
    AACCCGGAGGCTGACAAGGCAACGTCGGTGATCCGTACCGCCTGGTTGGACAAACGGCAGAAGGCGGCCTCGTCCGGTCCATCTACGCCGAGCACACTGGTGATAG
    CGCGCATCGGCATCGGTGCGGCCAGCCTGGAGACGACGTCCGCGGGCGTCTGGGTCAGTAACCCCCCCACCAGTTCTCGGGGGCAAGCTCCTCGAACCATCGGGGA
    CGTCTCCAACGCGCCACGCGCCATACCTGGTGCCAGTTGCTTGCGCATCCGGGTGTGCGGCCGGATCGGACGTCGCAGAAACGCAGCCACCCCCCCGTGAGAGTGA
    CCCACGGCGCTGGACACGTGTCTGGTTAC
    Clone Rv43
    :::::::::::::::Rv43SP6.seq:::::::::::::::
    CGGCCGGGATGTGCGCAATGGCAGGTTGTCGCCCGGCTTGATGTCGGCGTTAGCGCCGGATTCCACCACATCCCTGCGAAAGTCCGTTGGCGGTGCAATGATGTAN
    CGCTTCTCCCCATCGAGATAGTGGAGCAACGCAATCCGTGCGGTACGGTTCGGGTCGTACTCGATGTGCGCGACCTTGGCGTTGACACCATCTTTGTCATGGGGGC
    GAAAGTCGATCATCCGGTAAGCGCGCTTATGACCGCCGCCTTTGTGCCNGGTGGTAATCCGGCCATGCGCGTTGCGTCCACCGCGACCGTGCAGCCCCCCACCCAG
    CGACNTCTCCGGGGTTGACCGGGTGATCTCGGCGAAATCAGATACGCTGGCGCCGCGACGACCAGGCGTCGTGGCTTGTACTTGCGAAATTGCCATGGCTCTTTCA
    GGTCTTTCTCTCACCTCTCGTCGCCGGGCTAGGGCGCATTGCCTGCTCCT
    :::::::::::::::Rv43T7.seq:::::::::::::::
    TAGCGGTGTAACCAACTCCCGGTCACCACCCGCAAACCTCTTGCGGCAACAGCACCGTCGACGCGTCAACCGGGCTGCCCGGAATCCTGTGGATGGGGCATCGAGT
    GCATGGTCACGACGTCCCCGACGCGGCCGGTGGCAACGACAAGTGGCCCCGATGCACCACAAATGACGGCCCGCACACCGGTGGGGACGGCCAGCACGAGAGCCTG
    TCGCCGAAGTCGACGCTAATGCCGTAGGCATTGGCCGTCACAACAGCGCGACGCCCCCGCGTACCACCGAGTCCAGGNGGTTGGCGGTCTCCTCGGCCAACCAGGC
    GTGAACCCGGCGGATCCGAATGCGCAAGACCCGTGGGC
    Clone Rv44
    :::::::::::::::Rv44-2NDSP6.seq:::::::::::::::
    CCATTGGTCGGTGTGCGCATACCANTACNACGCGCCCGGGCACCTGACGCCGGCGGCCGCAACCATTCGGTGGCCATCGCCATCGTCTGCCACCCGGTCACCACGC
    ACCTTCTCCTGGCCGACCTAGTGCCGCCCACCGCCGCCGTTGCGTCCCATCGATCCGGTCAACATGAGCAGCGCCAACACCGAGCGGTACATGACATCTGCTGTGG
    AACCAGTGACANATTCCGCCGCCCATGATGATCNTCGACCGTCCTCCGGATTCGGTC
    :::::::::::::::Rv44-2NDT7.seq:::::::::::::::
    GCCGGCCTGGTCAAAGGGGCGTCCGAAGGANCCGGGCTGGGTAACAAGTTCCTGGCTCATATCCGCGAATGCGACGCCATTTGTCAGGTGGTGCGGGTGTTCGTCG
    ACGACNACGTGACTCATGTCACCGGACGGGTCGATCCCCAGTCCGACATTGAGGTCGTCGAGACCCGAGCTGATCCTGGCAGATCTGCAAACCCTGGAGCGCGGAC
    GGGCCGGCTGGAGAANGAAGCNGCACCAACAGGCGCGCAAGCCGGTCTACGACCCCGGC
    Clone Rv45
    :::::::::::::::Rv45SP6.seq:::::::::::::::
    GATCCACTGACCACGATGACATATCGAAATGCTCGACGATTCCGATGGCGATCAAGGCCACGATGCCCTGGCGTTGGGCGGTATCTGGTGGATGGTGTACCCCCGG
    GTAGGTTCCCGTGATCGTGTCGACCCAGTCCACGCGATGGGCGGCGAGGTCGTCGGGCACCGCATCAGGGGGTNTGGGGGAGTGGGGGTCGAGTTGGGGGGGGGGG
    AGCTCTCCCCCGGTAGAACTCTCACCGTTGGTCGCCGCGATCTTCTCTANCGTCGCCGCGTGGTCAGGAGGTAANCAGCTCACCGGTTCGGGCCCCGTCGTCCCCC
    GGGCATGAACGCATCTGCGAATCCGGGCTGGGATGCGAACAACGGACCTGTGCCG
    :::::::::::::::Rv45T7.seq:::::::::::::::
    TCTACTGCCGAATCGGGGAACGGTCCTCGCCCACCNGGTTCGTGTTGCCGGAATTACTCAGGCACCGGAAACGTCGAGAACTACGAGCGGAGTTGGACANAATACC
    GCTCCCNGGTGGGCGCCCCCATCGANTTGGGAAGCNGAAATGTGCTCTGGACCCCACCCAAGAATGACATTGCCGGCCGCCCTCCAACTGGAAATAGAANCNGTGA
    TCACCCGCCGCGTTCTTGGAAGGAATGGCATGCCCTGGGCCGGCGTTCCTTCCGCTGCCGACTCCTCCCACCAATTCACGCCGAAGGGCGTCCGTCTGC
    Clone Rv46
    :::::::::::::::Rv46SP6.seq:::::::::::::::
    ATACTCAAGCTTCTGTCACCGAAATCCCGCATGGGATAACGGGTTTAGATTTCGACAACGGGACCGTGTTTCTCAACAAGCCGTCATCAGCTGGGCCCGCGGACAA
    CGGTATCTACTTCACCCGCTTTCGCCCGT
    :::::::::::::::Rv46T7.seq:::::::::::::::
    CTGGCTCAAGCGCTCGGCGCGCAGGTGAACTCGGACCGGCTCGACGTCGCCGAACGCAAGCCCTGCTGGCCCACGGACCCGTCGTCATATGGGGGGAGGGGGGGGG
    TGCACAAGTCTACAACAACGCCGGCATCGCGTACAACGGCAACGTCGACAAGTCGGAGTTCAAGGACATCGAGCCGCATCATGCACGTCGACGTTGGGCGGTCCTC
    CACCGGGCCC
    Clone Rv47
    :::::::::::::::Rv47SP6.seq:::::::::::::::
    CCGCCCTCCGCATTATGGGTCAAGAACCATCGGGTCGGACTTCTGGGCTTCCAACGCTCGCGCCGTCCCN
    :::::::::::::::Rv47T7.seq:::::::::::::::
    CCGTGGCACTGTCAGCATATGCGCCGCTCCTCCTCATCGCTGCGCTCGGCATGCTGCCCCCCCCTCATGGCGTCACCCTACCCCAGCCGAACCGGAAAACGAGAAC
    GTGTTCCATTATTAGGGTGTGAGCACCAATACCAGATTGCTCACCAGGAACTCACGGCAGCACCGGGACCGGATGTCGGGCCAGAGCCCATCTCCCCTGGTAGGGG
    GAAATACCGCTAACGCGGCTCCGGTGCCG
    Clone Rv48
    :::::::::::::::Rv48SP6.seq:::::::::::::::
    TACTCAAGCTTGTCCAAATATCGAAGCGTCGGGTCGCGAGGCTCGGTCGGCAGCTCCAGCAAAACCCGCTCCACCCCTAGATGCCCGGTATCCTCAAGGTCTTTAT
    CCGCCGCTTCACCCCACTGGCACACGGTCACCGGCACGTCGCCCCCGGCCATGGCGCAACCGCTGAAGCGGACCCCGACAGCCCCTGCCCTGATGGACTGATCGGG
    GATCCACCCCGGCATTGAGCCGGGCTATCCGCGGGAAGTTCGCCGGTCCCCCCGCCCACATACAGCGGAGGATAGGGCTTGTCACCGGCTCGGCAGCAGTAGTCCC
    GGATCGAAGTCCACATATGTCCCATGGAATTCCGCCTGCTCCTGCGTTCAGATCTCGATTATCGCGCGCAACCGCTCATCGATCACACTGCCCCCACCCCCAGGGT
    CCACACCATGGTTGGCGACTTCTTCGCGCAACCAGCCACACCCACGCCGAAACGAAACCGTCCCTGCG
    :::::::::::::::Rv48T7.seq:::::::::::::::
    CAGCCATGCAAGCTTGGCCAACTCCTCATCGGACTTGAAGGTGCCGTCCTCGTTGGCGGCCCTGCTCCACGGCAGTTGATGGCACCAGGAATGTGTCCGGGGGGGG
    TGGCTTTGTTCCTGCGGCAGGTGCGCGGGGGCCAGGATCTTGCCGAGAACTCGTCGGGAGAGCGCAGTCGATGAGGTTCTTGAGCTTGATGGCCCCCCCCCCACCT
    CGTCGCGGAATGCCCGAATCGTGTTATCCGGCGGGANGCGGTGTAGGAAGTCACCGGCCGGCTGACCGGGTCGCTGGACAGGCCGGCTCCGTCGAGCTCC
    Clone Rv49
    :::::::::::::::Rv49SP6.seq:::::::::::::::
    ATACTCAAGCTTCAAAACAGGCCTGTTGTGGGCGACCCGGCTCGCCGAGTTCTCGACGCAACCCCCTCAACTGCCCCCCACCCCCCCCCATCTCCCCGGTCACGCA
    GGGGCCGCGGCCCGCGCCGCAGCGACGGCGTGTTCGCGCAGTTCGCCGTCAATGATGCTGACCTGATCGGGCCACCCCCCCTCTCCCCCTCGTCCCGTTCACTAAT
    CGCCGGTGCTCAGCAGCGTCTCGACAGCCACCACCCGAGTGGAGACCAGATGCNCCACCACGGACCCGCAGCGATGCCGATCACCTCACCCCGTCC
    :::::::::::::::Rv49T7.seq:::::::::::::::
    CAGGCATGCAAGCTTTGCAGTTGCTGAGTAATGTCGGCCAACGTCACCACAATCGCGATGAATTCAATCATGCCGCCCAGGCGGGCCAACCCAATGGTGGCCGGGA
    GCGGCAGCTCGATCGCAGCGCGGAGGTTGCCGGCCGCCAGTTGATTCACGAACAGGGTGAGGTCATAGGCGGGCAGGATAGTGACGAAGGCAAGACCTAGATCTGC
    CGTCGGAAGAAGAATCGAGTATCCGGTCGACACAACGGAAGCGAAAGTGCCGCGATGTTGATGAGCGTCGCCGGTTGTGGCGGCGGTGGCGGCGGTAGCACCGGTC
    CGCACATACCGCGGGCAACGCGGGCATCCGAATTTGGGGCAGGGTGTTCAAGGCGGCTGGCAACTCACCATGAATCT
    Clone Rv4
    :::::::::::::::Rv4SP6.seq:::::::::::::::
    CCGGCTCGTATGTTGTGTGGAATTGTGACCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACACTATAGAATACTCA
    AGCTTGGCCGCAGGGCCGAGTCGATTGGTCGCGGTCGCCTCGACAGTTAGCTTATGCAATGCTAACTTCGGGGCAAGTTCAGGCGGATCGGCCGATGGCGGGGCGT
    AGGTGAAGGAGACAGCGGAGGCGTGGAGCGTGATGACATTGGCATGGTGGCCGCTTCCCCCGTCGCGTCTCGGGTAAATGGCAAGGTAGCGCTGAGCTCGGGGGGT
    CGATTTGCCACCTGCTGCCGTGCCTGGGCATCGCGGTTTACCAGCGTAAACGTCCGCCGGACCTGGCTGCCGCCCGGTCTGGTTTCGCCGGCGTGACCCCCGGTCG
    CCATGACAGTGCGACCCTGNACCGGGCTGGCC
    :::::::::::::::Rv4T7.seq:::::::::::::::
    GTGTGCTGTCAATTCAGAGCTGAGCCTGATGCACTCAACTTACTGAGCATGCTAACGCTGGTCGTGCGGGTCTTGTTCCCGCGTGTCGGCAGGGCACACGCTCGGG
    GCGTAGCTGGGAGAGGCCCCGGTCAAGCCCGAGAGCAGTGCTCAGTCCGCCAGCTTGACCGACTTTCGATGAGAACGCGTTCTCGCCGTATTGAAGGGGGCGTTCG
    TGAGCCTGCGCGAGCAGCGCTCGCCGAGTGCGGCCGCTGATTCTTTCATCGAGCCAGGAGGCGCATTCGTGCTCGGGGGTGGGGGTGGGGGGGGGATCGTCGACGC
    GATCCGTCACCCACTCCTCGATCAGGTCTGCCTCATCGAACGGGCCAACGGTGCTGTCGGAGTAAGTGTGCGTGGGCACCGAGCCCCTGCTGTGGTACACCCCCAC
    CGTTGCATGAACAA
    Clone Rv50
    :::::::::::::::Rv50SP6.seq:::::::::::::::
    ATACTCAAGCTTCACCAGGCGCCCGGCGGGCGGGGGGGGAAGCCAGGCAGCCCCCTCGGGGGGTCGGGGGGTTGGGGGGGTCCCCCCCAGCAGTTCGATCTCTGGA
    TCGGCGGGGCTCTCCGGGCCGGCCTCGGCGACCTCAGCGGCCCGCGCTTCCCCCCCAACCAATTCCTAAGGGATAGATAACCCCCTCAAATGCAGGTTGGGGGGGG
    CAACCCGG
    :::::::::::::::Rv50T7.seq:::::::::::::::
    AGCTTCCGTCACGACCCGCCCTCGCCGGTGCCGGCGCCATCGGTCATCGGATCTCATGACGACGTCACGTAGGGCCCCCTAGCGGGGGGGGGGGGGGTCAACTGGC
    GAGGCGGCGGCGACGTGACTGAGCTGGCCGAGCTGGACCGGTTCACCCCGAACTACCGTTCTCGTCGACGACTTTCAGCAGGGGGGTTGCAGCCCCCTGGAAGGCG
    CCGGCCACGGTGTTGCTGGTGTGCGCGCCGACCGGCGCTGGCAAGACGGTGGTCG
    Clone Rv51
    :::::::::::::::Rv51SP6.seq:::::::::::::::
    ATACTCAAGCTTGCCGGGACCGCGGAACAGAACCGGCGGTTCCTACCGCGGTGTGCGGCCGGCGGATATCGGGCTCCCCCATAACCGAACCCCGATGTGGCGCTCC
    :::::::::::::::Rv51T7.seq:::::::::::::::
    ACGTTGGCTCTGCCGGAACGTATTTCCAGCGGCACGCATTCGGCGTGGGTGCCGGGCGGGATTGCGTCGCTGGGATCACCAGCAGTCCCCCCCCCCCCCTCCCCTC
    GGGCTATGAATTGCACCGAGCCGGAAAATCCNCAC
    Clone Rv52
    :::::::::::::::Rv52SP6.seq:::::::::::::::
    ATACTCAAGCTTGTCGTATTCCGTGGCACTGTCAGACATATGCGCCGCTCCTCCTCATCGCTGCGCTCGGCATCGTCGCCGGCGGTCATGGCGTCACCCTACCCAA
    GCCGAACGCGAAACGAGAACGTGTTCCATTATTAGGGTGTGAGCCAAATACCGATTGCTGAGGGGGGTCAGGGGGGGTTGTGCCCCCCCCCCCCCACCCCCCCCCC
    CCATCTGGGGTGGTAGCGGGGGA
    :::::::::::::::Rv52T7.seq:::::::::::::::
    CGTTGGTAGCCCGATATGCATAGTGTATCTTACTGAACATGATTTTCCATTATGGAGCCCGGGTGCCCCACCCCAGGTGCCCCCTCAGCCCCCCCCCCCCCCCACT
    GACCAGGTGTTGCGGGCGAACATCGGCCCCGGCTTCGGATTCCGGTCCGGGTACCGGGCGACCCACCGCTTCGAAGGTA
    Clone Rv53
    :::::::::::::::Rv53SP6.seq:::::::::::::::
    ATACTCAAGCTTGGCCAACTCCTCATCGGACTTGAAGGTGCCGTCCTCGTTGGCGGCCCTGCTCCACGGGCAGTTGATGGCACCCGGGTTGTTCCCGGGGCGGTGG
    CTTTTGTTCCTGCGGCAGGTGCGCGGGGGGCCATGATCTTGCCGGAAAACTCGTCGGAGAGCGCACGTCGATGAGGCTTCTTGACGTTGATGGCCCAGGACCTCGT
    CGCGGAATGCCCGAATCGTGTTATCCGCGGGGAGGCGGTGTATGAGGTCACCGGCCGGCTGACCGGGTCCCTGGACAGCGGGCGTCCGTCCAGCTCCCCACTTCTT
    GCGGGCGCCGTCCAACNACTTGACTTCTCCTGG
    :::::::::::::::Rv53T7.seq:::::::::::::::
    ATATCTTAAGCGTCGGGTCCCGAGGCTCGGTCGGCAGCTCCAGCAAAACCGCTCCACCCCTAGATGCCGGTATCCCTCAAGGTCTTTAGCGGGGTTCACCCCCCCA
    CTGGCACACCGGTCACCGGCACGTCGCCCCGGCATGGCGCAACCGCTGAAGCGCAACCCCGACAGCCCCCCTGCGGTGATGGACTGATGGGTCCCCCCCCCCCTTG
    AGCCGGGCTATCCGCGGGAAGTTCGCCGGTCCCCCGCCCACATACAGCGGAGGATAGGCTTTGTCACCGGCTTCGGCCAGCAGTAGATCGGATCGAAGTCCCACAT
    ATGTCCCATGGAATTCCGCCTGCTCCTGCGTCCAGATCTCGATTATCGCGCGCAACCGCTCATCGATCACACGTCCGCCACCCGAAATCCCACCCCACATGGTGGC
    GACTTCTTCGCGCA
    Clone Rv54
    :::::::::::::::Rv54SP6.seq:::::::::::::::
    ATACTCAAGCTTGTCGCGGTAAACCCGCGAGCAGGGCGGTGGGTGCGGTGCAAAACAACCACACTTCTTTGCGGGTCGGTGATCTGCAGGGGGAGGGGGGGGGGGA
    CCACCATGCGCGCTAAATCGGCGATCAGCGCGTCGGCTATCGCCTGGTGCCGCCCACCGGAATCGGCCAGCCGACCGAATGGCCAGCGTTGCCAGCCCCATCAGTC
    CGGCGCCGGCCGACACCAGTGACGGCAACGGTGAAATCGCGTGGGCGGCAACGCCGGTGAACAACGCGCGGGGCATCCTGGCCCCCAGCGACCCCCAGGGTTTGGT
    GCCCTGGGGCCAGCATCCGCAGCCCGAGACGCAGGACCGAGCCCAGTGCAGTAGGCAAAGACCGCTTGTCGGAGACATGAACTCCACGACCGT
    :::::::::::::::Rv54T7.seq:::::::::::::::
    AGCTTATTGAACCGCGGGTCGCAGGCAAAGTGGACCTCATAACGACTCGGGTCCAGCGACCGCGCCCAACACGAACGGCCGGAACGACGTGGGGAGGGGTGCCCCT
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CTCCATACTCGCCCCTAATCTCGAGGCAGCCCGTACCCGCAGGCAACCTCCAAAAAATGCAATCCCCAAAATTCAATGCGTCGAGCTATTTCTCACACCGACCCCG
    CTAGTTGCGGATCAGAAATCCGTTGGGCGCGGAAGTCCAGCCGATTTGTTCTCCCGCTCCGCATCATGCTTGTAATCGTTGGAAATTCATCCTCATATGCCCCTGC
    ATCGCTTCATAGGGTCCAGGCCAAACCGGGCA
    Clone Rv55
    :::::::::::::::Rv55SP6.seq:::::::::::::::
    CTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGGGAAGCTATTTAGTGACACTATAGAATAC
    TCAAGCTTGGCCACTCCGCGGTGTGTGGTGGACCCATCTGAGCATGTGCCAAAACCGGGGCAGACAGTCCCAATTGACGTGAGCCCCCCCTCACTTGCTGGGGTAG
    CGTCG
    :::::::::::::::Rv55T7.seq:::::::::::::::
    TAGCGCCCCTCCCGGGCCGGAGCTCCACGGCGTGGATCAAGGTACCGGCCCGGGATGTTGCCATGGAGTTGTTGCCCCTTGATGTCCCCTTACCCCCCCCCCCCCA
    TTCCACCACATCCCCTTGCGAAAGTCCGTTGGGTGCAATGATGTAGCGCTTCTCCCATCGAGATAGTGGAGCAACGCAATCCGTCCCCTACCCCTTCGGGGTCTAC
    TCCCCCGATGTGCCGCACCTTGGCGTTGACACCATCTTGTCATTGCGGCAGGGGGATCATCCGGGTAAAATTATGAGGGGGGGTTGTGCCCCTATTAACCCCCCCC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    ACCAAGCGTCGTGGGCTTGTTCTTGCGAAATTGCATGTCTAATCAGGTCTTTCTC
    Clone Rv56
    :::::::::::::::Rv 56SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    TTCCGGGTTGACAAGCAGGCCAACCTGCACTTCTC
    :::::::::::::::Rv56T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    TTGTACTTGCGAATTGCCATGTCTAATCAGGTCTTTCTC
    Clone Rv57
    :::::::::::::::Rv57SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    AAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    :::::::::::::::Rv57T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CACCACCACCTCCGCCGGCCCTCGCTGAGGTCC
    Clone Rv58
    :::::::::::::::Rv58SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CGCGCTGCTGTTCGCGCTGTCGGCCAGCTCGCAGGACGCGCTGCGGCAAACCGCCGCGCCCGTGGGATTGGGTCT
    :::::::::::::::Rv58T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CTCGCGGCCGGTGGTGGCCTGGAATGTCCANCGCCGTTCACCTACGTGACCTGATGGATCCGGGGGGNT
    Clone Rv59
    :::::::::::::::Rv59SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    ACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    :::::::::::::::Rv59T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CGTGACGGGTACTTGGCGCGATAACGTGGTG
    Clone Rv5
    :::::::::::::::Rv5SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    GTCGCGGGCGCCACCGAGGTCGTTGACCACGA
    :::::::::::::::Rv5T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    TCCGATCCCCGCGGCTTCAGG
    Clone Rv60
    :::::::::::::::Rv60SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    GATGCCGACCGAAATACGACCCACATGGCTCTTGTTGCNCAGTGTTGGCGGCATCAAATACCCTCAGTGCCGTCCGAC
    :::::::::::::::Rv60T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    TCGCGCACTTCGCGATCGCGTGACTGGTCCTCGCGATGCCCCTGCGTAGC
    Clone Rv61
    :::::::::::::::Rv61SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    :::::::::::::::Rv61T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    ATAGCTCGACCGGATCGGAGAGAATCTGGTTCTCGT
    Clone Rv62
    :::::::::::::::Rv62SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCGAACGGTTGCCAGCCCCGGC
    :::::::::::::::Rv62T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    ACCGATCTTTTCGTCGAAAATGGCGGTCGTGTCGGGGGTATACGTCCGACATCCCCAGCCCCTCCCGCTGAGGTGA
    Clone Rv63
    :::::::::::::::Rv63SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    GGGCGGGCAACCGTCCGCTCAAGTCGCTTTCCGATCTGCTCA
    :::::::::::::::Rv63T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    AATCAGAAACCTGGCGCGCGACACAGCGTGTACTTGC
    Clone Rv64
    :::::::::::::::Rv64ZP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CGGGAGTTGCTCGTCGGCCGACCGACCCGGCCAGCCGGGGCAAAACCGGGGAGATTCAGGGGTGGGTAGGGGTCTGGGGGGGGTGG
    :::::::::::::::Rv64T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    TCTCTCGATGCGGTCTCCACCACCGAACAG
    Clone Rv65
    :::::::::::::::Rv65SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    GGTCCACTCACGGTACCGGGTATTTTATCGTCGACGAGGGGCACATGGT
    :::::::::::::::Rv65T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    GCAGCCCAATGAGTTGCCGCGCGGGCTGCATCGCGGCCGGACGAGG
    Clone Rv66
    :::::::::::::::Rv66SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    ATTGCTCGTCGGCCGACCGACCGGCCCAGCCGGGGCGGCAAAACCCCGGAAGA
    :::::::::::::::Rv66T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CAATCGCGGCTCACTTGNGCGATNGTCNAACAACCAACGCGNCCTGGTCACCCTCAAAGAAAATGGGTGT
    Clone Rv67
    :::::::::::::::Rv67SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCGGCGGGGCT
    :::::::::::::::Rv67T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    GCTGAGCCGCAGCTGATCCGGGCTCGCCGCGGCGTGATCCTGGCCTGTGGTGGTTTCGAGCATAACGAGCAGATGCGAAT
    Clone Rv68
    :::::::::::::::Rv68SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CATTTCAGCCCCCACACCCCCAGACTTGCTCTCGATGNCCCCG
    :::::::::::::::Rv68T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    TTTGGACCGAACGGGATTCATACTCCGCAGGCCGGGGCCCCTCCGAAAATCGGCACATGTCCTTTGATCGTTCGCAACAN
    Clone Rv69
    :::::::::::::::Rv69T7D3.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CGATCGTCACAAAAAATCTCCGTCCT
    Clone Rv6
    :::::::::::::::Rv6SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CGGTAGGCACCCCAATCGACCCCTACCCCGGCTATCTCACCCCCGATCTCCAGGCTCCGCCGATCCATGCGCATCCGGTCCGGATCCC
    :::::::::::::::Rv6T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    TTGTTCTTTTGCCGGATGGCCGTGTTTGCTGACATATCGGCGCGCCGCCCCCCCCCCCC
    Clone Rv70
    :::::::::::::::Rv70SP6D2.seq:::::::::::::::
    NCTACGCTGCTGAATGTTGTGCGCCGGAGGANCTCAAGACCCACGCGGTTGTACGCCGGACNTGGCGACTGGTCAGGGGGGA
    :::::::::::::::Rv70T7D3.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    C
    Clone Rv71
    :::::::::::::::Rv71SP6.seq:::::::::::::::
    CTAAGCTTTCGGGTCCGCCGCCACTAGTACCGCGTTGCCGGCCCCCCGCCGACCTAGAATGTTCCGCCCCATTGCCGTTCCTCCCCGCCCCCCGGTT
    :::::::::::::::Rv71T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    TGTTCCGCATACGATT
    Clone Rv72
    :::::::::::::::Rv72SP6D2.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    :::::::::::::::Rv72TD3.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    TTGCGCTGGG
    Clone Rv73
    :::::::::::::::Rv73SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    GCCACAAACGCGCCCCCGGTATAC
    :::::::::::::::Rv73T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CGCACCGCGATCGGTCTGTCGCGGGCATGTCCAAACAACTGAAATCCCGTCAACATGGTCGAC
    Clone Rv74
    :::::::::::::::Rv74SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    ACGTACCTCCGACGGCCAGCTGACGAACGGGTTTTGACGGA
    :::::::::::::::Rv74T7D3.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    GGNACACTNCGGTTCACGTTAATCANCATCGCGANTTNCTNCGTNTTCGATTANTTCTGCTAACGNNTCNNNNGTCCCCTGGGGTAGAG
    Clone Rv75
    :::::::::::::::Rv74SP6D2.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    :::::::::::::::Rv75T7D3.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    ACTGGTCTAGCCACACCGGGGAGAATNCNCGACCCGGNGCTATCGACCCNATCACGGCTTGTCGNAAMGATAGNCAGCC
    Clone Rv76
    :::::::::::::::Rv76SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    GGCGCCCCGCGAGTTCTAGCACAGCAACGCCGCGGCCACCACAGGGGGCG
    :::::::::::::::Rv76T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCC
    Clone Rv77
    :::::::::::::::Rv77SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    TTGGCGCCCCAACGCCCCCGGCAGCATCGACGTGGGTAGAGCTGGATGCCGCCAGCCACGGGGGGTGGGACAACCCCCCGAGCTGCGGGGGACCCCCCCG
    :::::::::::::::Rv77T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    ACCCCGAAGGGCCGCTGGCCCGGCGGAACCTTGTCCTCCA
    Clone Rv78
    :::::::::::::::Rv78SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    TGNTNGGATCNGCCGACGTGCANCTGAANGANTATCCCCGGGCTGTNCCACNAGGTGTTCAACGAACCGGANCGCAACCAAGTG
    :::::::::::::::Rv78T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    TGACA
    Clone Rv79
    :::::::::::::::Rv79SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    GCCGTCCTTGAAGAAACCGGTGGCGGNGGCAACCC
    :::::::::::::::Rv79T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    AACGGCACCANGCGCAACCGAAGGGCCAATCCGACATCGG
    Clone Rv7
    :::::::::::::::Rv7SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCTGTTGGCCGACAATCTCGGTGACTTCGCGGTCAAGTCACCCGGGCCGTTGCGGGTGTCCGCAGCCTTGTCCAGCCCCCTCGCTTACTCCA
    :::::::::::::::Rv7T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    TTCAGCCATCGGGGACATCATGCTCGCTTCATACTCCTCGACCAGTCGGCGGAACAGCTCGATTCCCGGAACGCCCACGCATGGTG
    Clone Rv80
    :::::::::::::::Rv80SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    TCATCACCACCATCGCTAGC
    :::::::::::::::Rv80T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    TTCGCCGCCGACG
    Clone 81
    :::::::::::::::Rv81SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    :::::::::::::::Rv61T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCACAACCCGTCGCGATCCAGCTCAGCCACGATCTCCACAATGCGCCCATCAATCGCATTGCGCTGAACGGGCAACTCCGCCAACTCCTCCAA
    Clone Rv82
    :::::::::::::::Rv82SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    GTTATTTTCCTCGCTTCCGGGCTGTCATCATTAAACTGTGCAA
    Clone Rv83
    :::::::::::::::Rv83SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    GANTTCTCCNGGGTTGAACCGGTNATCT
    :::::::::::::::Rv83T7.seq:::::::::::::::
    TGTGTGTGGTGGTAACCCATCTGAGCAGTGTGCCAAACCGGGGCAGCCAGCTCCCCAATTGACGTGAGCCCCGCTCACTTGCTGGGTAAGCGTCG
    Clone Rv84
    :::::::::::::::Rv84SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCGACNAACGGCACTCCTTTTTTCCNTTTGCTCTTTC
    :::::::::::::::Rv84T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    GCCGTGGAAATATCAGCAATGATA
    Clone Rv85
    :::::::::::::::Rv85SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    AACACTCCGGCGGCGGCAGCGTGATCAACATCAGCTCCACCATGGGCCGGCTGGCGGCGGGGGGTTTC
    :::::::::::::::Rv85T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    AGATCGCGCTGACCACACTCAACGCCCGGACGGCTGTCCCTACCGGCGATCCAACCGGAGT
    Clone Rv86
    :::::::::::::::Rv86SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCGAAATGCTGCGCAACATGCTCTAC
    :::::::::::::::Rv86T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CGGGTCCGGCAGCNAACCCGCCCGCGCCGATACCCGCTGCCCCGCGTGCGTGATTGACCGCCGCGCGCACGCTGGCCANGGATCAAACGGGCT
    Clone Rv87
    :::::::::::::::Rv87SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    TAGAATCTTTCCGCGGGCGGGTGAATCTCTGCNGGATCGGGGCNGGCGC
    :::::::::::::::Rv87T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    GATGGGCAGTCGCCGCTCGGCATCGTCAGCCTCGCCGC
    Clone Rv88
    :::::::::::::::Rv88SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    AGGGCTTCGTGCGGACCATCAACATCAAGTTCCAAGACGCCTACAACGCCNGTGGCGGCCCACAACCGCTGTTCGACTTCCCGG
    :::::::::::::::Rv88T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    TCCAGCACTCGGTCGATG
    Clone Rv89
    :::::::::::::::Rv89SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    GATATTCNGCCNTCCCANCTCGAGCCTGACNCTNGATATCGTCGANNCTCACCATCNCGATCNGCTGTGCCGGTNTTGCTCGGACTN
    :::::::::::::::Rv89T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    ATCGGCANACTCNCGTCGATGTGCCGCGGCGCAACCATCCCCCACAATGATCNGGTGCGTCTGATCAGGCN
    Clone Rv8
    :::::::::::::::Rv8SP6D.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    TTGTGCGACCCTCAACGCCATTGCCGGC
    :::::::::::::::Rv8T7D4.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    GACACCGGCGAGACCACTTAGGCAGTGACGGCCT
    Clone Rv90
    :::::::::::::::Rv90SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    TCGCCNATGGCTCCNGCGNNTCGCNCAACGTNTGGTTTGGTNANCTGCCTACCTGGTCNT
    :::::::::::::::Rv90T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    GTACCNTCATGTCGGTCTGCGCNCGATATTGACGATCGTGTTCCCACGANNNNNNGANCCTCATCACGCCGGTTCGAGTGCCG
    Clone Rv91
    :::::::::::::::Rv91T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    AANCGGCGCTNANNANATNCGCGNGAATGNCCGTCCACNANTGCATGG
    :::::::::::::::Rv91T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    TGCCAGCTCGACCTGACGCTGGAAATCG
    Clone Rv92
    :::::::::::::::Rv92SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    GANCCCGAGCNATNCNANGTCCGTCAATC
    :::::::::::::::Rv92T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CACATAAGACGCAAACTGCTCTATTGTCAANTCCCANCGTGGTGTGGCNCATGAAGATGTTTGG
    Clone Rv94
    :::::::::::::::Rv94SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    GTTGGCANTNACCTCNGAGCCCCATTGGACATCANCCCANCCCGCCTGCGAACGGGAACGTCAGCNCNCTGGCGACAACCTGGCCAACAN
    :::::::::::::::Rv94T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    GCATGGCCGTCTGGGGTCANCTCNACNATTTCANCCGTTGACCCGGGGTCACA
    Clone Rv95
    :::::::::::::::Rv95SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    GGCTCTACGCGCCATCTTCCCCGGATTCTTCGCGGCNGNGTTNCCGNGGGACCCCCGGACTGTGACNGGCCAACGGGCTCATCATCG
    :::::::::::::::Rv95T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    TGTCTGCGANCTCGCGCACCAGCTCGACGGCCCGGGCA
    Clone Rv96
    :::::::::::::::Rv96SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    GTAAGCAAAATACGT
    Clone Rv9
    :::::::::::::::Rv9SP6.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CGCCGCACCCGACCGCGG
    :::::::::::::::Rv9T7.seq:::::::::::::::
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
    GGCCGGCGCCGGCTGGTCGGCAAGGAAGACCCGTTCCGGTGCTGAT
  • TABLE 4
    End sequences of the polynucleotide inserts cloned in the named
    recombinant BAG vectors contained in the I-XXXX M. bovis strain
    Pasteur genomic DNA libraiy.
    RvXXXSP6 corresponds to the SP6 endsequence of the clone RvXXX;
    RvXXXT7 corresponds to the T7 endsequence of the clone RvXXX.
    RvXXXIS 1081 corresponds to a region located close to a copy of the IS1081
    repetitive sequence (Insertion element)
    The character << - >> denotes an uncertain base residue.
    Clone X0001
    ::::::::::::::::X0001SP6.seq::::::::::::::::
    AAG-
    TCGGGTTTCCACACGCGCGGTTTGACCCTAGTCATATGTAATCATGTGTACCATGTGCGGGGCGCTTTTCGACGGC
    CGCGAAGGACCGGA-ATTTCCTGTGATTTCACTGCATGCGTACCATCTGGCACAATTGAGCA-TTGTCT-TCGCGG
    TGGTCGG-CGGGTTGCGTGCCGCCTGCTGCGA-ATGCACCA-TAAGCCCGAACCCACCGGCTTGGTGACCACCCGC
    ACGCTGCGTGTGGGGGGTAACCACTCCGCGACCCCAAGGATGGTCATTTCCAATGAACCGGCTGGACTTCGTCC
    A—A
    ::::::::::::::::X001T7.seq::::::::::::::::
    GTCGCGGTTCGATCGACCCGATCTTCACCTCGTAACCTCGATGCTTAGCAGGATCCAGCTTGACCGCGTTTGGCT
    CTACCCACTCTTTGAGTGGCGCCGTCGCCTGTGCCCCATCGGTGTTCATGACGAACGCTTCGAAAGACTTCCTCT
    TGTGAGCCGGAATGTCTGCGTAAAGAAGTTCCATGTCCGGGAAGTAGACCCGGTCGCCCTCCACGTGGTACTCC
    TTCGAGGTCCGCTTCTCGCCGGATCCGATAAACACCGGCCCCAGGCACCGCAGCGTGAGTTCGAACGGCTTCAG
    GTAGGTGTTCATGCGGCGGACTCCGGGAGTGCGAGAAATAGCGGTCGCGCGTAGCTGTAGACCGGATGGTTTCC
    GCCCAGGCTGACGTCGAAGATGCCTCCTTGGAAGGGGCGCGA
    Clone X00002
    ::::::::::::::::X0002SP6.seq::::::::::::::::
    AACTCAAGTTTTTACGGTGATCGCGCATCACCTGGTTCATGAACTGGAAGCAGCGCAGCGCTTCCTTTTCGGCCG
    CAACATGAGCCAGCCTCTCGTCGGCGGTCGGGTGCAGGTGCTCGGGCAGCTCGGCCGCGACAGCCGCCTGACC
    CTGAAACCAGCTTCCATATCCCGCGAC-
    AACGACGCCAGTCCGCTACGTAACCCCTCCGCGACTGTCCATGGACAACAGCGCGTTTCTCCACCGACCGGGCCC
    GGGTGT
    ::::::::::::::::X0002T7.seq::::::::::::::::
    GTGCAGGTTTCGACAATGTGGTGCCGGTTCGGCGGCTACGTGCCATCGAGACACTGGCGCA-GCTATCGCACCC
    GTTATCGGCTGCGAGCAAATCGCGGTATGCGTTCTTGAGCATGAGTCGGCGACCGTCGTCATGGTCGACACCCA
    CGACGGAAAGACGCAGATCGCCGTCAAGCATGTGTGCCGCGGATTATCAGGACTGACCTCCTGGCTGACCGGCA
    TGTTTGGTCGCGATGCCTGGCGCCCGGCCGGCGTGGTCGTGGTCGGCTCGGATAGCGAGGTCAGCGAATTCTCG
    TGGCAGCTCGAAAGGGTCCTGCCGGTGCCGGT
    Clone X0003
    ::::::::::::::::X0003SP6.seq::::::::::::::::
    TTCGAGTCATGCGCCCGCCTCGACCACGAA-ATGCACGTCG-GGTTCGATCGACCCGATCTTCACCTCGTAACCTC
    GATGCTTAGCAGGATCCAGCTTGACCGCGTTTGGCTCTACCCACTCTTTGAGTGGCGCCGTCGCCTGTGCCCCAT
    CGGTGTTCATGACGAACGCTTCGAAAGACTTCCTCTTGTGAGCCGGAATGTCTGCGTAAAGAAGTTCCATGTCC
    GGGAAGTAGACCCGGTCGCCCTCCACGTGGTACTCCTTCGAGGTCCGCTTCTC
    ::::::::::::::::X0003T7.seq::::::::::::::::
    GTCATGTGTACCATTTGCGGGCGCTTTTCGACGGCCGCGAAACACCGGAGATTTCCTGTGATTTCACTGCATGCG
    TACCGTCTGGCACAATTGAGCAGTTGTCTGTCGCGGTGGTCGGCCGGGTTGCGTGCCGCCTGCTGCGAGATGCA
    CCAATAAGCCCGAACCCACCGGCTTGGTGACCACCGCACCGCTGCGTGTGGGGGGTAACCACGCCGCGACCCCA
    AGGATGGTCATTTCCAATGAACCGGCTGGACTTC-TCAACAA
    Clone X0004
    ::::::::::::::::X0004T7.seq::::::::::::::::
    AACAGCGCGGTTGAACTGATAGGTGCGGCCCGGCTCGAGCAGGCCGGGCCATTGTTCGATGCGGTTACCGAAA
    GATCTCTTCGGTGACCTGCCCGCCGCCGGCCAGCTCGGCCCAGTGCCCGGCGTTGGCCGCCGCGGCGACGATCT
    TGGCGTCCACGGTGGTCGGGG
    Clone X0006
    ::::::::::::::::X0006T7.seq::::::::::::::::
    GCATCTGGGCTGGCGGTGGTTCGCCGCTCCGAAGCCGTCGAACACCATCGCCAGCGCGGCTTCCACATCAACGA
    CCATTTCGGCCAGCTTGCGGCGCATCAGCGGCTTGTCGATGAGCGCCCCACCGAATGCCCGCCGCTGCCCGGCG
    TA-CACAGCGATTCGACCAGCGCGCGGCGCGCGTTGCCGAGGGCGAACGAAGCGGTGCCCAACCGCAATCTGTT
    GGTCAGCTCCATCATGCGGGTGAGTCCCTTGCCG
    Clone X0007
    ::::::::::::::::X0007SP6.seq::::::::::::::::
    ATCGGTTTCCAGCAACAGCCGATCGACGGCTTCGCCCA-GGCCGCTCCCGGGCGACCCGACCATTGCGTGCGCC
    GCGTAACGCCATCACGGATGACGCGCAGTTCGTCGCTGTCTAGCTCCACCATCGCCTGCACACCGGCGGCCAG-
    ACCCATTGGCCGTCGCACTCGTA-
    AGCAGGTAATCCTCGTCGACGGACTCGGTAACCACCGCCGCCAGCTCCGCTGCCAGGTCGGCGGGGTTGACACC
    GGCGGGCATCGGGATGGACGACGACGCGGTGCTGACGGCGCCTGTC
    ::::::::::::::::X007T7.seq::::::::::::::::
    AGCGGTTTCCCA-GCGGGATGTGCTGTGAGCGCCGCACCACCAGCGCCGACGCTAAGGATGGAACGCACGGCAT
    CTTCTGACGCGTAACCGCGTTGTGATCGCGAGCTGAGGAGACGGTATGGGGGAGGGTTCTCGGAGGCCATCTGG
    GATGTTGATGTCTGTCGATCTTGAGCCGGTGCAACTCGTCGGCCCGGACGGTACGCCGACGGCCGAACGCCGCT
    ACCACCGTGACCTTCCTGAGGAAACGCTGCGTTGGCTCTACGAGATGATGGTGGTCACCCGCGAGCTGGATACC
    GAATTCGTCAATCTGCACG
    Clone X0008
    ::::::::::::::::X0008SP6.seq::::::::::::::::
    CAAGCTTCCACAGGTAGGGATCGAGGAACAGCGCGTTGAACTGATAGGTGCGGCCCGGCTCGAGCAGGCCGGC
    CATTTGTTCGATGCGGTTACCGAAAATCTCTTCGGTGACCTGCCCGCCGCCGGCCAGCTCGGCCCAGTGCCCGG
    CGTTGGCCGCCGCGGCAACGATCTTGGCGTCCACGGTGGTCGGGGTCATGCCCGCGAGCAGGATCGGCGAGCG
    GCCGGTCAGCCGGGTGAACTTCGTCGAAAGCTTGACCCTGCCGTCGGGGAGGCGAACCACGGTCGGTGCGTAN
    CTCCACCAAGCCCGGGCAACCTCGGGGGTGGCGCC
    ::::::::::::::::X0008T7.seq::::::::::::::::
    TGGACCTCATGACAACGCGGCGGCGATTACCCCCGCTACCGCCAGCAGCATGACGGCGGTAGCGAACACCGCC
    GGATGCAGCGCAGGTGCGTCGATGTGCTCACGGAATCGCCCCGGCACCGCGATCTCGAGGATCACCAGTGCCAC
    CCCCTGCAGCGCGACACCGACGATTCCGTACACCGCCACGCCGATCAGGCCCTGGGCCAGCTGGCGTATATGGC
    GGCGATGGTGACGATGGCCAGCGCCACATACATTGTGGCGGCCAGAACCACGGCGTTGGGGCGGCGGTCGATG
    AACACTAGGCGACGCAGATCGCCCGGGGTCAACAGGTTGACCATCAGAAAGCCTGCGA
    Clone X0009
    ::::::::::::::::X0009SP6.seq::::::::::::::::
    TTTGGTGCGGCCGGCAATCAACTTC-
    GCTC-CAGCGGTTTCCCAGGCGGGATGTGCTGTGAGCGCCGCACCACCAGCGCCGACGCTAAGGATGGAACGCA
    CGGCATCTTCTGACGCGTAACCGCGTTGTGATCGCGAGCTGAGGAGACGGTATGGGGGAGGGTTCTCGGAGGC
    CATCTGGGATGTTGATGTCTGTCGATCTTGAGCCGGTGCAACTCGTCGGCCCGGACGGTACGCCGACGGCCGAA
    CGCCGCTACCACCGTGACCTTCCTGAGGAAACGCTGCGTTGGCTCTACGATATGATGGTGGTCACCCG
    ::::::::::::::::X0009T7.seq::::::::::::::::
    CGCCCAGGGCCGCTCCCGGGCGACCCGACCATTGCTGTCGCCGCGTAACGCCATCACGGATGACGCGCAGTTCG
    TCGCTGTCTAGCTCCACCATCGCCTGCACACCGGCGGCCAGGACCCATTGGCCGTCGCACTCGTAGAGCAGGTA
    ATCCTCGTCGACGGACTCGGTAACCACCGCCGCCAGCTCCGCTGCCAGGTCGGCGGGGTTGACACCGGCGGGC
    ATCGGGATGGACGACGACGCGGTGCTGACGGCGCCTGTCGCGACGCTGAGCTCGGACACAGCTAGTAAATGTA
    GCCTAACCTACTTAATGGGTCGCAGCCCCCCGGGGTCGTCGCATGTCCAACGTTGCTCGACTGGAAGAAAATGC
    TCGTCGGGGAGCAAATGGCACC
    Clone X0010
    ::::::::::::::::X0010SP6.seq::::::::::::::::
    AATACTCAATCTTGATCGGTTTCCAGCAACAGCCGATCGACGGCTTCGCCCAGGGCCGCTCCCGGGCGACCCGA
    CCATTGCTGTCGCCGCGTAACGCCATCACGGATGACGCGCAGTTCGTCGCTGTCTAGCTCCACCATCGCCTGCA
    CACCGGCGGCCAGGACCCATTGGCCGTCGCACTCGTAGAGCAGGTAATCCTCGTCGACGGACTCGGTAACCACC
    GCCGCCAGCTCCGCTGCCAGGTCGGCGGGGTTGACACCGGCGGGCATCGGGATGGACGACGACGCGGTGCTGA
    CGGCGCCTGTCGCGACTCTGAGCTCGG
    ::::::::::::::::X0010T7.seq::::::::::::::::
    GGATGTGCTGTGAGCGCCGCACCACCAGCGCCGACGCTAAGGATGGAACGCACGGCATCTTCTGACGCGTAACC
    GCGTTGTGATCGCGAGCTGAGGAGACGGTATGGGGGAGGGTTCTCGGAGGCCATCTGGGATGTTGATGTCTGTC
    GATCTTGAGCCGGTGCAACGCGTCGGCCCGGACGGTACGCCGACGGCCGAACGCCGCTACCACCGTGACCTTCC
    TGAGGAAACGCTGCGTTGGCTCTACGAGATGATGGTGGTCACCCGCGAGCTGGATACCGAATTCGTCAATCTGC
    AGCGCCAGGGGGAAGCTGGCGTTGTACACGCCCTGTCGCGGGCAGGAAGCCGCGCAGGTGGGTGCGGCGGCTT
    GCCTACGCAAAACCGACTGGTTGTTCCCC
    Clone X0012
    ::::::::::::::::X0012SP6.seq::::::::::::::::
    ATCACGACAACAGCGACGGTGTGTCGGATCAGCGGCCCCCGTTGCCGGGCAATGTTGAGGCGTTTCTGCGTCTG
    GTTGAGGCCGGCTGGGAC-
    CCGAGGTGGCTCGTCGGCCACATGGGCAGCACACCACCGTGGTGATGCATCTAGACGTGCAGGACCGTGCCGCT
    GGCCTGCA
    ::::::::::::::::X0012T7.seq::::::::::::::::
    GCGGCTACGTGCCATCGAGACACTGGCGCAGGCTATCGCACCCGTTATCGGCTGCGAGCAAATCGCGGTATGCG
    TTCTTGAGCATGAGTCGGCGACCGTCGTCATGGTCGACACCCACGACGGAAAGACGCAGATCGCCGTCAAGCAT
    GTGTGCCGCGGATTATCAGGACTGACCTCCTGGCTGACCGGCATGTTTGGTCGCGATGCCTG
    Clone X00013
    ::::::::::::::::X0013T7.seq::::::::::::::::
    TACAAGCGGCACCTCGCCGGTGAACTGACCGTTCGCACGCTGCGCACCGCCGCCGGGCGCGTGCTCGGCGCGC
    CGGCGGCCCCCGAGGCCTGAGAGGGGAACCAACCATGCAGGTGAACATGACGGTAAACGGCGAGCCCGTCACC
    GCCGAGGTCGAACCCCGGATGCTGCTGGTCCATTTTCTCCGTGATCAGCTGCGGCTCACCGGAACTCACTGGGG
    CTGTGATACCAGCAACTGCGGGACATGCGTGGTGGAGGTCGACGGCGTGCCGGTGAAATCCTGCACGATGCTCG
    CCGTGATGGCCTCCGGGC
    Clone X0014
    ::::::::::::::::X0014T7.seq::::::::::::::::
    AGCGGCTGGTTACGACTCCCTGTTTGTGATGGACCACTTCTACCAACTGCCCATGTTGGGGACGCCCG-
    CC-TCCGATGCTGGAAGCCTACACTGCCCTTGGTGCGCTGGCC-C-GCGACCGAGCGGCTGCAACTGGGCGC-
    TTGGTGACC-GCAATACCTACCGCACCCC-ACCCTGCTGG-CAAA-
    ATCATCACCACGCTCGACTTGGTTAGCGCCGGTCGA-CGATCCTCGGCATTGGAACCGGTTGGTTT-
    Clone X0015
    ::::::::::::::::X0015SP6.seq::::::::::::::::
    ACGCGCGCCGATCATATCTGCTATGGATGTACAATTCAGCTCTTGCTGTTATACCAGTATATGGTGTACTATTTG
    ATCTATGCTGACGTGTGAGATGCGGGAATCGGCCCTGGCTCGACTCGGCCGGGCTCTGGCTGATCCGACGCGGT
    GCCGGATTCTGGTGGCGTTGCTGGATGGCGTTTGCTATCCCGGCCAGCTAGCTGCGCACCTCGGGTTGACCCGA
    TCGAATGTGTCCAACCATCTGTCGTGTTTGCGGGGCTGCGGGCTGGTA-
    TCCCAACCTATGAGGGCCGGCAGGTTCGGTAT
    ::::::::::::::::X0015T7.seq::::::::::::::::
    CCGCGCTGCTGCTGACGTCGGTCGAACGTGCGACACGTCTGCGAATACCGGCCGAACGCTGGGTTTATCCACAG
    GCTGGCACCGACGCCCACGACACACCGGCCGTCGCCGACCGCCACCGACTGCATCGGTCGACGGCCATTCGGAT
    CGCCGGTGCCCGGGCGCTGGAACTGGCTGGGCTGGGGCTCGATGACATCGAATACGTCGACCTGTATTCGTGCT
    TTCCCTCCGCTGTCCAAGTCGCCGCAATCGAACTCGGCCTGGACACCGACGATCCTGCCCGCCCGCTGACCGTC
    ACCGGGGGCCTGACCTTCGCCGGCGGGCCGTGGAGCAATTACGTCACGCACTCCAT
    Clone X0016
    ::::::::::::::::X0016SP6.seq::::::::::::::::
    CAGGCGTGCAATGACCTGCACTGCGCCGGA-A-TCCCTAACCCACTAAACCGGGGCCGCTCACAAGCCGTGCAGC
    TCGGTCAGCGTCAGGTGCGCGACCAGGAA-
    TAAATGAGCAGACCCGTGCCGTCAACGATGGTGGCGATCATCGGCCCCGAAACGATGGCCGGGTC-
    ATGCGCAACTTCTTCAGCAGCGGCGGAAGGACGGCA-CCACCAGCGAC-ACCACACCACGAT
    ::::::::::::::::X0016T7.seq::::::::::::::::
    GCGAA-CACTTCGTCAACTTCCAGGGCTGCCCGCACCAAGTATTTCGACGAGTATTTCCGTCGGGCCGCCGCCGC
    CGGCGCGCGGCAGGTGGTCATCCTGGCGGCGGGGCTGGACTCGCGCGCGTACCGGCTGCCTTGGCCCGACGGG
    ACCACGGTTTTTGAGCTGGACCGCCCGCAGGTCCTTGATTTCAAGCGCGAGGTGCTCGCCAGCCACGGTGCCCA
    ACCGCGCGCCCTGCGCCGCGAGATCGCCGTCGACCTGCGTGACGATTGGCCACAAGCCTTGCGGGACAGTGGTT
    TCGATGCGGCTGCACCGTCGGCATGGATTGCCGAAGGGCT
    Clone X0017
    ::::::::::::::::X0017SP6.seq::::::::::::::::
    TTGGGC-TTGCCC-CAAATA-GGCCCCAATCAAAAGCCGAGCAGGTGGAACCTA-CGATTCGCCTC-TCGT-TGTGC
    ACCCGAGCCATCGCACGCGCGGGGAATTCCCGGAT-TC-CCGTATTCTCCGGCGGCCGGGCTAACCCATCCCA-GCC
    GAACGGTTGGCTC-TGCCGTGGGTCCCGTGTTGGCCGATCGGGGCGTCACCGGGGTGCTCGGGTGCGG-
    TGACCATGGC-AACTGCCCC-ATGGGCCGACCCTGGTGCAGATAAACCTG
    ::::::::::::::::X0017T7.seq::::::::::::::::
    TGGTGGAGGTCCCCACCAA-ACCCGGCCGTAACTCTGCTCACGGAAATGCGG-CAGGCCGCGCGTAGCACGTGGT
    ATCCGCCATAAAGGTGCACCTTAAGCACGGCGTCCCAATTCTCGAACGACATCTTGTGGAAGGTGCCGTCGCGC
    AAGATCCCGGCGTTGCTCACCACACCGTGCACGGCGCCGAATTCGTCAAGCGCGGTCTTGATGATGTTCGCTGC
    GCCGTCCTCGGTGGCGACGCTGTCGGTA-TTGGCGACCGCCCGGCCCCCCTTGTCGCGAAATCTCGGCGACGAC
    CTCATCGGCCATCGCCGAACCGGGCGCCCG
    Clone X0018
    ::::::::::::::::X0018SP6.seq::::::::::::::::
    GCCGGCCAAACTGGCCGGCGGGGTTGCTGTC-TCAAGGTGGGTTCCGCCACCAA-ACC-
    CACTCAAGGATCGCAAGGAAAGC-
    TCAAGGATGCGGTCGCGGCCGCCAAGGCCGCGGTCAAGGAGGGCATCGTCCCTGGTGGGGGA-CCTCCCTCATC
    CACCAGGCCCGCAAGGCGCTGACCGAACTGC-TGCGTC-C-GACCGGTGACAA-GTCCTCGGTGTCCACGTGT-CTC
    CGAAGCCCTTGCCGCTCCGTTGTTCTGGATC-CC-CCAAC-CTGGCTTGGACGGCTC-
    GTGGTGGTCAACAAGGTCAGCGAGCTACCCGCCGGGCATGGGCTGAACGTGA
    Clone X0018
    ::::::::::::::::X0018T7.seq::::::::::::::::
    CGACCT-
    AATTGTCCTGTAATGCCCAGCTCACCAA-GCATGGCTGGTGGCCGGGGCGGTGAAGCCGGCGTCTGCGGCACCG
    TCCAACTC-ATGTGGAT-GCCGGAATGGGGATGTCCGG-ACGGCGAATCCGTA-
    TTCGCTTGTCCCGTGAGGCCCAGGTGGATGGGGGGAAGGATC-TGGTGTCCGGGATGAT-
    ATGGGGCCGATGCCGCCGGTTGAAGTCCACTGGATCGGGAATTCGGGAATCGTGAT-
    CCGACGTTCAGGCCGAAC
    Clone X0019
    ::::::::::::::::X0019SP6.seq::::::::::::::::
    CTAACGGAATGAAAGCCCTGGTGGCCGT-TCGGCGGTGGCCGTCGTCGCACTGCTCGGTGTATCTTCCGCCCAAG
    CTGATCCCGAGGCGGATCCCGGCGCAGGTGAGGCCAACTATGGTGGCCCCCCAAGTTCCCCACGTCTTGTCGAT
    CACACCGAATGGG-CGCA-
    TGGGGAATTCTGCCCAGCCTCCGGGTCTACCCGTCCCAAGTTGGGCGTACA-CCTCCCGCCGCCTCGGGATGGCC
    GCTGCCGACCCGGCCTGGGCC-AGGTTCTCGCGCTGTCACCGGAAGCCGACACTGCCGGC
    ::::::::::::::::X0019T7.seq::::::::::::::::
    CCGCGGGACAC-CCTC-ATGCTGCCGCCATGGACGCGGTCGAACGCAAGCAGCTGATCGAGCTACAACGCCGCG
    CGGAACGCTTCCGCCGCGGGCGTGACCGCATCCCGTTGACCGGGCGGATCGCGGTGATCGTCGATGACGGCATC
    GCCACCGGAGCGACGGCCAAGGCGGCGTGCCAGGTCGCCCGGGCGCACGGTGCGGACAAGGTGGTGCTGGCG
    GTCCCGATCGGCCCA-ACGACATCGTGGCGAAGATTCGCCGGGTACGCCGATGATGTGGTGTGTTTGGCGACGC
    CGGCGTTGT
    Clone X0020
    ::::::::::::::::X0020T7.seq::::::::::::::::
    CTCTGGGACCGGCCACGGTGCC-CCGGCGTTCCCGGACGTGCTGCGCCAGGTGTCCGGCGGCCGCGTGCATGGT
    GTTCCCGGATCGGCCGCTGGCCAGAGCCCACCGGTGAATCTGGCGCCTGGCCGACCACCGTGCGCCGTAGGCTT
    GCGATCGTGCAGCGCTGGCGTGGCCAGGACGAGATCCCGACGGATTGGGGCAGATGCGTGCTCACCATCGGGG
    TATTTGACGGCGTGCACCGCGGGCACGCCGAACTGATCGCGCACGCGGTCAAAGGCGGC
    Clone X0021
    ::::::::::::::::X0021SP6.seq::::::::::::::::
    AATACTCAAGCTTTCGTCAGTTCATTGCGCCAGCAGACCAACAA-AGCATCGGGACATACGGA-TCAACTACCCG
    GCCAACGGTGATTTCTTGGCCGCCGCTGACGGCGCGAACGACGCCAGCGACCAC-TTCAGCAAATGGCCA-CGCG
    TGCCGGGCCACGAGGTTGGTGCTCGGCGGCTACTCCCAGGGTGCGGCCGTGATC-ACATC-TCACCGCCGCACCA
    CTGCCCGGCCTCGGGTTCACGCAGCCGTTGCCGCCCGCAGCGGAC-
    ATCACATCGCCGCGATCGCCCTGTTCGGGAATCCCTC-GGCCGCGCTGGCGGGCTGATTAAC
    ::::::::::::::::X0021T7.seq::::::::::::::::
    TGCCGCGGATTTGGCTGGCTGCCCAATATTCAGAATCGGGCCTTTCTTTTTGCGCGACAATAAGGTCACAGTAAA
    CCCTCGTTTTGTGAGATGCGGGGCGGGCCGGGCGAA-TCGACCTCGAGTGAATGGATCTCGAGTGAATGGACAG
    GGCATCGCCTACGAGTCGCATCCCCATCCAACAGACCGGTGCTCTTGCATCGGACCCTGAAGGTCCCGCACGGA
    GGGTGTGGTTGCCGGCGCGGGGTCACGGTGCGGTAGCGACGTAGTGTTTGAACGAATTTCTTGATGCTCCAACC
    TGTTTGGTGTTCAATCCAGTTCT
    Clone X0175
    .....X0175SP6.....
    AA-CTTGCGCGCTCGGCCGGGTC-AGCATCCAGCTGCTCGGCAAGGAGGCCAGCTAC-C-TCGCTGCGTATGCCCA
    GCGGTGAGATCCGCCGGGTC-ACGTCCGCTGCCGCGCGACCGTCGGCGAAGTGGGCAATGCCGAGCAGGCAAAC
    ATCAACTGGGGCAAGGCCGGTCGGATGCGGTGGAAGGGCAAGCGCCCGTCGGTCCGGGGCGTGGTGAT-AACC
    CGGTC-ACCACCCGCACGGCGGTGGTGAGGGTAAAACCTCCGGCGGCCGTCACCCGGTTAGCCCGTGGGGCAA
    .....X0175T7.....
    A-TCGAAAGTGACCATCTCTACCTTGAGTGCCATACCGCCCGACCCTATGCCTCGGATAGCTCGGCGGAAAGAAA
    CGCTTGCAGTGCCGCCGAATAGGCGGCTACGTCGTGAGCGCCCATCAACTCTCGCGCGGAGTGCATCGCCAGCT
    GGGCGGCGCCGACGTCGACCGTGGGGATTCCGGTGCGCGCCGCGGCCAACGGCCCGATCGTCGACCCGCACGG
    CAGATCGGCGCGATGTTCGTAACGCTGCATAGGCACTCCCGCGCGCTGGCAGGCCAGTGCGAACGCCGCCGCG
    GTGCGTCCG
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      Figure US20060063182A1-20060323-P00900
      Non-radioactive Labeling and Detection of Biomolecules
      Figure US20060063182A1-20060323-P00901
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Claims (5)

1-41. (canceled)
42. A method of detecting Mycobacterium tuberculosis in a sample, comprising:
(a) contacting the sample with a first nucleic acid, wherein the first nucleic acid is a polynucleotide sequence comprising SEQ ID NO:2, a fragment of SEQ ID NO:2 comprising at least 8 consecutive nucleotides of SEQ ID NO:2, or a polynucleotide whose sequence is complementary to SEQ ID NO:2 or to the fragment of SEQ ID NO:2; and
(b) detecting hybridization between the first nucleic acid and a second nucleic acid in the sample, wherein detecting hybridization between the first and second nucleic acids indicates the presence of Mycobacterium tuberculosis in the sample.
43. A method of detecting Mycobacterium tuberculosis in a sample, comprising:
(a) contacting the sample with a first nucleic acid, wherein the first nucleic acid is a polynucleotide sequence comprising SEQ ID NO:3, a fragment of SEQ ID NO:3 comprising at least 8 consecutive nucleotides of SEQ ID NO:3, or a polynucleotide whose sequence is complementary to SEQ ID NO:3 or to the fragment of SEQ ID NO:3; and
(b) detecting hybridization between the first nucleic acid and a second nucleic acid in the sample, wherein detecting hybridization between the first and second nucleic acids indicates the presence of Mycobacterium tuberculosis in the sample.
44. The method of any one of claims 42 or 43, wherein the first nucleic acid hybridizes to the genome of Mycobacterium tuberculosis but not to the genome of Mycobacterium bovis under stringent hybridization conditions.
45. The method of claim 44, wherein the stringent hybridization conditions comprise hybridizing at 65° C. in 6×SSC, followed by washing at 65° C. in 2×SSC.
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