CY1109058T1 - A METHOD FOR ISOLATING A POLYNUCLEOTIDE INTERESTED FROM THE GENEALM OF A MYCOBACTERIUM USING A BAC-BASIC DNA. APPLICATION TO DETECTION OF MYCOBACTERIES - Google Patents

A METHOD FOR ISOLATING A POLYNUCLEOTIDE INTERESTED FROM THE GENEALM OF A MYCOBACTERIUM USING A BAC-BASIC DNA. APPLICATION TO DETECTION OF MYCOBACTERIES

Info

Publication number
CY1109058T1
CY1109058T1 CY20091100505T CY091100505T CY1109058T1 CY 1109058 T1 CY1109058 T1 CY 1109058T1 CY 20091100505 T CY20091100505 T CY 20091100505T CY 091100505 T CY091100505 T CY 091100505T CY 1109058 T1 CY1109058 T1 CY 1109058T1
Authority
CY
Cyprus
Prior art keywords
bac
mycobacterium
isolating
mycobacteries
genealm
Prior art date
Application number
CY20091100505T
Other languages
Greek (el)
Inventor
Stewart Cole
Roland Buchrieser-Brosch
Stephen Gordon
Alain Billault
Original Assignee
Institut Pasteur
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut Pasteur filed Critical Institut Pasteur
Publication of CY1109058T1 publication Critical patent/CY1109058T1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/35—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Mycobacteriaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1072—Differential gene expression library synthesis, e.g. subtracted libraries, differential screening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

The present invention is directed to a method for isolating a polynucleotide of interest that is present or is expressed in a genome of a first mycobacterium strain and that is absent or altered in a genome of a second mycobacterium strain which is different from the first mycobacterium strain using a bacterial artificail chromosome (BAC) vector. The invention further relates to a polynucleotide isolated by this method and recombinant BAC vector used in this method. In addition the present invention comprises method and kit for detecting the presence of a mycobacteria in a biological sample.
CY20091100505T 1998-04-16 2009-05-11 A METHOD FOR ISOLATING A POLYNUCLEOTIDE INTERESTED FROM THE GENEALM OF A MYCOBACTERIUM USING A BAC-BASIC DNA. APPLICATION TO DETECTION OF MYCOBACTERIES CY1109058T1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/060,756 US6183957B1 (en) 1998-04-16 1998-04-16 Method for isolating a polynucleotide of interest from the genome of a mycobacterium using a BAC-based DNA library application to the detection of mycobacteria
EP99913534A EP1071797B1 (en) 1998-04-16 1999-04-16 A method for isolating a polynucleotide of interest from the genome of a mycobacterium using a bac-based dna library. application to the detection of mycobacteria

Publications (1)

Publication Number Publication Date
CY1109058T1 true CY1109058T1 (en) 2014-07-02

Family

ID=22031547

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20091100505T CY1109058T1 (en) 1998-04-16 2009-05-11 A METHOD FOR ISOLATING A POLYNUCLEOTIDE INTERESTED FROM THE GENEALM OF A MYCOBACTERIUM USING A BAC-BASIC DNA. APPLICATION TO DETECTION OF MYCOBACTERIES

Country Status (10)

Country Link
US (7) US6183957B1 (en)
EP (2) EP2058325A3 (en)
AT (1) ATE423212T1 (en)
AU (1) AU3164099A (en)
CY (1) CY1109058T1 (en)
DE (1) DE69940427D1 (en)
DK (1) DK1071797T3 (en)
ES (1) ES2322521T3 (en)
PT (1) PT1071797E (en)
WO (1) WO1999054487A2 (en)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6183957B1 (en) 1998-04-16 2001-02-06 Institut Pasteur Method for isolating a polynucleotide of interest from the genome of a mycobacterium using a BAC-based DNA library application to the detection of mycobacteria
FR2791067B1 (en) 1999-03-16 2003-01-31 Pasteur Institut SEQUENCE DELETEES IN M.BOVIS BCG / M.OU M.TUBERCULOSIS, METHOD FOR DETECTION OF MYCOBACTERIA USING THESE SEQUENCES AND VACCINES
US7052844B2 (en) * 1999-12-21 2006-05-30 Ingeneus, Inc. Purification of DS-DNA using heteropolymeric capture probes and a triplex, quadruplex or homologous duplex binding mechanism
CA2404988A1 (en) * 2000-04-11 2001-10-18 Institut Pasteur Listeria monocytogenes genome, polypeptides and uses
US20020164634A1 (en) * 2000-08-26 2002-11-07 Perlegen Sciences, Inc. Methods for reducing complexity of nucleic acid samples
US20020137043A1 (en) * 2000-08-26 2002-09-26 Nila Patil Method for reducing complexity of nucleic acid samples
AU2002302919A1 (en) * 2001-02-22 2002-10-03 Institut Pasteur Comparative mycobacterial geneomics as a tool for identifying targets for the diagnosis, prophylaxis or treatment of mycobacterioses
AU2002317940B9 (en) * 2001-06-22 2008-06-05 The Secretary Of State For Health Mycobacterial antigens expressed under low oxygen tension
EP2196473A1 (en) * 2001-07-04 2010-06-16 Health Protection Agency Mycobacterial antigens expressed during latency
WO2003018053A1 (en) * 2001-08-22 2003-03-06 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Vaccine against mycobacterial-induced diseases
US7439346B2 (en) * 2001-10-12 2008-10-21 Perkinelmer Las Inc. Nucleic acids arrays and methods of use therefor
GB0125535D0 (en) * 2001-10-24 2001-12-12 Microbiological Res Authority Mycobacterial genes down-regulated during latency
US20040023237A1 (en) * 2001-11-26 2004-02-05 Perelegen Sciences Inc. Methods for genomic analysis
US20090124514A1 (en) * 2003-02-26 2009-05-14 Perlegen Sciences, Inc. Selection probe amplification
US20060183132A1 (en) * 2005-02-14 2006-08-17 Perlegen Sciences, Inc. Selection probe amplification
US20050100911A1 (en) * 2003-08-06 2005-05-12 Perlegen Sciences, Inc. Methods for enriching populations of nucleic acid samples
ITRM20030411A1 (en) * 2003-08-29 2005-02-28 Consiglio Nazionale Ricerche USE OF SPECIFIC GENE SEQUENCES OF MYCOBACTERIUM TUBERCOLOSIS IN ADDITIONAL PROTEINS FOR DIAGNOSIS AND PREVENTION OF TUBERCULAR INFECTION.
WO2005044086A2 (en) * 2003-10-30 2005-05-19 Tufts-New England Medical Center Prenatal diagnosis using cell-free fetal dna in amniotic fluid
AU2005299413A1 (en) 2004-10-22 2006-05-04 Revivicor, Inc. Ungulates with genetically modified immune systems
EP1910410B1 (en) * 2005-07-01 2011-10-26 Forsyth Dental Infirmary for Children Tuberculosis antigen detection assays and vaccines
CA2638904A1 (en) * 2006-01-20 2007-07-26 University Of South Florida Methods for profiling transcriptosomes
RU2341288C1 (en) * 2007-05-10 2008-12-20 Общество С Ограниченной Ответственностью "Биотэк" MEDICINE FORMING CELLULAR IMMUNITY FOR MYCOBACTERIUM TUBERCULOSIS H37 Rv, METHOD OF PRODUCTION THEREOF (VERSIONS), RECOMBINANT STRAIN AND TUBERCULOSIS DIAGNOSTIC AGENT
DK2348827T3 (en) 2008-10-27 2015-07-20 Revivicor Inc IMMUNICIPLY COMPROMATED PETS
CN102656279A (en) * 2009-12-17 2012-09-05 凯津公司 Restriction enzyme based whole genome sequencing
US8865404B2 (en) * 2010-11-05 2014-10-21 President And Fellows Of Harvard College Methods for sequencing nucleic acid molecules
WO2015083056A2 (en) * 2013-12-03 2015-06-11 Kusuma School Of Biological Sciences Genetic markers for diagnosis of tuberculosis caused by mycobacterium tuberculosis

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2422956A1 (en) 1978-04-13 1979-11-09 Pasteur Institut METHOD OF DETECTION AND CHARACTERIZATION OF A NUCLEIC ACID OR OF A SEQUENCE OF THE SAME, AND ENZYMATIC REAGENT FOR THE IMPLEMENTATION OF THIS PROCESS
FR2518755B1 (en) 1981-12-23 1986-04-11 Pasteur Institut PROBE CONTAINING MODIFIED NUCLEIC ACID RECOGNIZABLE BY SPECIFIC ANTIBODIES AND USE OF THE SAME TO DETECT AND CHARACTERIZE A HOMOLOGATED DNA SEQUENCE
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4868105A (en) 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
US5202231A (en) 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
US5776693A (en) * 1990-06-08 1998-07-07 Institut Pasteur Specific detection of the mycobacterium tuberculosis
EP0554437B1 (en) 1991-07-31 2000-04-05 VYSIS, Inc. Nucleic acid probes for the detection of shigella
US5474796A (en) * 1991-09-04 1995-12-12 Protogene Laboratories, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
JP3478396B2 (en) 1992-03-04 2003-12-15 ジェネンコア インターナショナル インコーポレーテッド Production of black Aspergillus catalase-R
EP0631635B1 (en) 1992-03-04 2001-09-12 The Regents Of The University Of California Comparative genomic hybridization (cgh)
US6641814B1 (en) * 1997-04-02 2003-11-04 Statens Serum Institut Nucleic acids fragments and polypeptide fragments derived from M. tuberculosis
NL192999C (en) 1994-11-17 1998-07-03 Lederverwerkende Ind De Buffel Link.
MX9801687A (en) * 1995-09-01 1998-11-29 Corixa Corp Compounds and methods for diagnosis of tuberculosis.
GB9526178D0 (en) * 1995-12-21 1996-02-21 Taylor John Hermon Novel polynuceotides and polypeptides in pathogenic mycobacteria and their use as diagnostics,vaccines and targets for chemotherapy
EP0907751A2 (en) * 1996-04-29 1999-04-14 Gesellschaft für Biotechnologische Forschung mbH (GBF) Dna, rna and a protein useful for detection of a mycobacterial infection
US6183957B1 (en) 1998-04-16 2001-02-06 Institut Pasteur Method for isolating a polynucleotide of interest from the genome of a mycobacterium using a BAC-based DNA library application to the detection of mycobacteria

Also Published As

Publication number Publication date
US7842781B2 (en) 2010-11-30
EP2058325A3 (en) 2009-10-28
US8455187B2 (en) 2013-06-04
WO1999054487A2 (en) 1999-10-28
US20050250104A1 (en) 2005-11-10
EP1071797A2 (en) 2001-01-31
PT1071797E (en) 2009-05-20
EP2058325A2 (en) 2009-05-13
AU3164099A (en) 1999-11-08
ES2322521T3 (en) 2009-06-22
US7112663B1 (en) 2006-09-26
US6183957B1 (en) 2001-02-06
US6991904B2 (en) 2006-01-31
DK1071797T3 (en) 2009-06-02
US6492506B1 (en) 2002-12-10
EP1071797B1 (en) 2009-02-18
US20060063182A1 (en) 2006-03-23
US20070092884A1 (en) 2007-04-26
ATE423212T1 (en) 2009-03-15
US20030198974A1 (en) 2003-10-23
DE69940427D1 (en) 2009-04-02
WO1999054487A3 (en) 1999-12-09

Similar Documents

Publication Publication Date Title
CY1109058T1 (en) A METHOD FOR ISOLATING A POLYNUCLEOTIDE INTERESTED FROM THE GENEALM OF A MYCOBACTERIUM USING A BAC-BASIC DNA. APPLICATION TO DETECTION OF MYCOBACTERIES
EP1961826A3 (en) A method for direct nucleic acid sequencing
ATE147794T1 (en) CHEMICAL METHOD FOR ANALYZING DNA SEQUENCES
EP0188450A4 (en) Methods for the in vitro detection and identification of unknown pathogens or genetic entities.
ATE373726T1 (en) METHOD FOR DETECTING A DNA TARGET MOLECULE
WO2000077260A8 (en) Genomic profiling: a rapid method for testing a complex biological sample for the presence of many types of organisms
DE60035691D1 (en) Method for recognition of single nucleotide polymorphisms
DE69807293T2 (en) Multiplex PCR for detection of infections caused by Mycobacterium avium complex
AU4663997A (en) Detection and identification of non-polio enteroviruses
ATE474933T1 (en) METHOD FOR DETECTING PATHOGENIC ORGANISMS
WO1995033854B1 (en) Use of a specific marker for detection of salmonella with pcr
EP2116617A3 (en) Probes, probe sets, methods and kits pertaining to the detection, identification and/or enumeration of bacteria
CA2324298A1 (en) Method for typing of hla alleles
WO2001014592A3 (en) Identifying organisms by detecting intronic nucleic acid or encoded proteins
ATE90971T1 (en) METHOD FOR ANALYZING A SPECIFIC SEQUENCE OF DNA OR RNA, REAGENTS AND KITS THEREOF.
ATE409237T1 (en) DETECTION OF NUCLEIC ACID DELETION SEQUENCES
EP0739987A3 (en) Oligonucleotides for detecting Salmonella species and detection process using the same
DE59107264D1 (en) Methods for the detection and quantitative determination of Nitrosomonas strains in waste water or soil
SE9500341D0 (en) Method for detecting DNA sequence variations
WO1999000517A3 (en) Biosensor materials and methods
ATE197481T1 (en) METHOD AND REAGENTS FOR TYPING CLASS I HLAGENS
EA199800438A1 (en) METHOD AND SET FOR QUANTITATIVE DETERMINATION AND DETECTION OF MICROORGANISMS
ATE513061T1 (en) DIAGNOSTIC METHOD AND KIT FOR DETERMINING THE KELL BLOOD GROUP GENOTYPE
KR20040076292A (en) biosensor for detecting BTEX and the manufacturing method thereof
AU5858499A (en) Oligo nucleotides, method and kit for detecting (listeria monocytogenes) by amplifying and/or hybridizing nucleic acids