SE9500341D0 - Method for detecting DNA sequence variations - Google Patents

Method for detecting DNA sequence variations

Info

Publication number
SE9500341D0
SE9500341D0 SE9500341A SE9500341A SE9500341D0 SE 9500341 D0 SE9500341 D0 SE 9500341D0 SE 9500341 A SE9500341 A SE 9500341A SE 9500341 A SE9500341 A SE 9500341A SE 9500341 D0 SE9500341 D0 SE 9500341D0
Authority
SE
Sweden
Prior art keywords
nucleic acid
sequence variations
dna sequence
mismatch
solid support
Prior art date
Application number
SE9500341A
Other languages
Swedish (sv)
Inventor
Ulf Landegren
Arild Lagerqvist
Original Assignee
Ulf Landegren
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ulf Landegren filed Critical Ulf Landegren
Priority to SE9500341A priority Critical patent/SE9500341D0/en
Publication of SE9500341D0 publication Critical patent/SE9500341D0/en
Priority to JP8523465A priority patent/JPH10513055A/en
Priority to CA002211666A priority patent/CA2211666A1/en
Priority to EP96902038A priority patent/EP0871766A1/en
Priority to PCT/SE1996/000095 priority patent/WO1996023903A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method of analyzing nucleic acid-containing samples for sequence variations relative to standard nucleic acid sequences, which method comprises providing a single-stranded standard nucleic acid sequence immobilized on a solid support, hybridizing a nucleic acid strand derived from a sample to the immobilized strand, subjecting the nucleic acid complex formed to (i) mismatch-induced cleavage or (ii) mismatch-terminated extension reactions, and detecting possible cleavage or extension-termination by optical measurement on the solid support.
SE9500341A 1995-01-30 1995-01-30 Method for detecting DNA sequence variations SE9500341D0 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
SE9500341A SE9500341D0 (en) 1995-01-30 1995-01-30 Method for detecting DNA sequence variations
JP8523465A JPH10513055A (en) 1995-01-30 1996-01-30 Methods for detecting changes in nucleic acid sequence
CA002211666A CA2211666A1 (en) 1995-01-30 1996-01-30 Method for detecting nucleic acid sequence variations
EP96902038A EP0871766A1 (en) 1995-01-30 1996-01-30 Method for detecting nucleic acid sequence variations
PCT/SE1996/000095 WO1996023903A1 (en) 1995-01-30 1996-01-30 Method for detecting nucleic acid sequence variations

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE9500341A SE9500341D0 (en) 1995-01-30 1995-01-30 Method for detecting DNA sequence variations

Publications (1)

Publication Number Publication Date
SE9500341D0 true SE9500341D0 (en) 1995-01-30

Family

ID=20397030

Family Applications (1)

Application Number Title Priority Date Filing Date
SE9500341A SE9500341D0 (en) 1995-01-30 1995-01-30 Method for detecting DNA sequence variations

Country Status (5)

Country Link
EP (1) EP0871766A1 (en)
JP (1) JPH10513055A (en)
CA (1) CA2211666A1 (en)
SE (1) SE9500341D0 (en)
WO (1) WO1996023903A1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5770370A (en) * 1996-06-14 1998-06-23 David Sarnoff Research Center, Inc. Nuclease protection assays
EP0843019A3 (en) * 1996-11-08 2002-10-09 Kyowa Medex Co., Ltd. Method for determination of specific nucleic acid sequence, and a reagent therefor
AU6293798A (en) * 1997-01-29 1998-08-18 Institut Pasteur Polynucleotide associated with the bo or bor syndrome, its corresponding polypeptide and diagnostic and therapeutic applications
DE1051515T1 (en) * 1998-01-27 2001-04-19 Cytocell Ltd MODIFIED NUCLEIC ACID PROBES AND THEIR USE
DE19911130A1 (en) 1999-03-12 2000-09-21 Hager Joerg Methods for identifying chromosomal regions and genes
US7166432B2 (en) 1999-03-12 2007-01-23 Integragen Compositions and methods for genetic analysis
WO2001085988A1 (en) * 2000-05-09 2001-11-15 Diatech Pty. Ltd. Methods for detecting nucleic acid molecules having particular nucleotide sequences
WO2003070977A2 (en) * 2002-02-21 2003-08-28 Nanogen Recognomics Gmbh Method for detecting single nucleotide polymorphisms
WO2004061100A1 (en) * 2002-12-10 2004-07-22 Olympus Corporation Method of analyzing nucleic acid mutation and method of analyzing gene expression

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU3919293A (en) * 1992-03-27 1993-11-08 University Of Maryland At Baltimore Detection of gene mutations with mismatch repair enzymes
US5750335A (en) * 1992-04-24 1998-05-12 Massachusetts Institute Of Technology Screening for genetic variation

Also Published As

Publication number Publication date
WO1996023903A1 (en) 1996-08-08
JPH10513055A (en) 1998-12-15
CA2211666A1 (en) 1996-08-08
EP0871766A1 (en) 1998-10-21

Similar Documents

Publication Publication Date Title
SE9601318D0 (en) Method for nucleic acid analysis
DK0668777T3 (en) Prostate-specific membrane antigen
WO1999005315A3 (en) Nucleic acid sequence analysis
WO2000055368A3 (en) Probe/mobility modifier complexes for multiplex nucleic acid detection
FI950487A (en) Method, reagent and test kit for detecting and amplifying nucleic acid sequences
EP1961826A3 (en) A method for direct nucleic acid sequencing
WO2000077260A8 (en) Genomic profiling: a rapid method for testing a complex biological sample for the presence of many types of organisms
FR2422956A1 (en) METHOD OF DETECTION AND CHARACTERIZATION OF A NUCLEIC ACID OR OF A SEQUENCE OF THE SAME, AND ENZYMATIC REAGENT FOR THE IMPLEMENTATION OF THIS PROCESS
DK0979308T3 (en) Procedure for Preparing a Diagnostic Standard Gene Transcript Pattern
ATE188744T1 (en) METHOD FOR INCREASING THE DETECTION SENSITIVITY OF NUCLEIC ACID HYBRIDS (TARGET/SAMPLE)
AU2002360222A1 (en) High troughput analysis and detection of multiple target sequences using circular probes
BG98660A (en) Biosensor method on the basis of gene samples
DE60035691D1 (en) Method for recognition of single nucleotide polymorphisms
NO852022L (en) NUCLEIC ACID HYBRIDIZATION ANALYSIS USING DETECTABLE ANTI-HYBRID ANTIBODIES.
FI951082A (en) Improved method for the quantification of nucleic acid
SE9500341D0 (en) Method for detecting DNA sequence variations
HK1068377A1 (en) Analytical method and kit
DE69129192T2 (en) METHOD AND REAGENTS FOR THE CARACTERIZATION OF HLA-DRBETA DNA
HUP0303377A2 (en) Method for the detection of nucleic acid molecules
DE68907305T2 (en) Method of analyzing a specific sequence of DNA or RNA, reagents and kits therefor.
DE60043618D1 (en) METHOD FOR DETECTING A MOLECULAR DETECTION RESPONSE
EP1055735A3 (en) Method for gene analysis by hybridisation in the presence of a double-stranded DNA-binding protein
DE60015980D1 (en) BIOSENSOR SYSTEM WITH INCREASED SENSITIVITY BY MEANS OF MOLECULAR AMPLIFICATION OF THE SIGNAL
ATE328116T1 (en) METHOD AND CARRIER FOR BIOLOGICAL ANALYSIS USING OLIGONUCLEOTIDES CARRYING AN ENZYMATICALLY ACTIVABLE MARKER
SE0101934D0 (en) New methods