CY1107428T1 - Αφαιρετικη υβριδοποιηση με βαση μικροδιαταξεις - Google Patents

Αφαιρετικη υβριδοποιηση με βαση μικροδιαταξεις

Info

Publication number
CY1107428T1
CY1107428T1 CY20081100274T CY081100274T CY1107428T1 CY 1107428 T1 CY1107428 T1 CY 1107428T1 CY 20081100274 T CY20081100274 T CY 20081100274T CY 081100274 T CY081100274 T CY 081100274T CY 1107428 T1 CY1107428 T1 CY 1107428T1
Authority
CY
Cyprus
Prior art keywords
identification
removal
nucleic acids
present
library
Prior art date
Application number
CY20081100274T
Other languages
English (en)
Inventor
Steven Perrin
Katherine Call
Timothy Connolly
Original Assignee
Aventis Pharmaceuticals Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aventis Pharmaceuticals Inc. filed Critical Aventis Pharmaceuticals Inc.
Publication of CY1107428T1 publication Critical patent/CY1107428T1/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Ceramic Products (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Η εφεύρεση αφορά μια μέθοδο υψηλής απόδοσης και αποτελεσματικότητας για τον προσδιορισμό και την εξάλειψη των πλεοναζόντων στοιχείων σε έναν πληθυσμό από μόρια νουκλεϊκού οξέος με τη χρήση μικροδιατάξεων. Η μέθοδος αυτή περιλαμβάνει ένα πρωτόκολλο επαναληπτικής αφαίρεσης χρησιμοποιώντας μια βιβλιοθήκη που επηρεάζεται περισσότερο από άγνωστα γονίδια με κάθε μετέπειτα γύρο. Η αφαίρεση των επαναλαμβανόμενων και προηγουμένως χαρακτηριζόμενων νουκλεϊκών οξέων από τη βιβλιοθήκη επιτρέπει τον προσδιορισμό mRNA χαμηλής αφθονίας από πηγές ενδιαφέροντος και ενισχύει τον ρυθμό ανακάλυψης νέων γονιδίων. Η παρούσα εφεύρεση είναι επίσης ωφέλιμη για την αφαίρεση των μολυσματικών νουκλεϊκών οξέων από τις βιβλιοθήκες κλωνοποίησης.
CY20081100274T 1999-05-19 2008-03-07 Αφαιρετικη υβριδοποιηση με βαση μικροδιαταξεις CY1107428T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/314,698 US6638717B2 (en) 1999-05-19 1999-05-19 Microarray-based subtractive hybridzation
EP00936091A EP1185699B1 (en) 1999-05-19 2000-05-19 Microarray-based subtractive hybridization

Publications (1)

Publication Number Publication Date
CY1107428T1 true CY1107428T1 (el) 2012-12-19

Family

ID=23221053

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20081100274T CY1107428T1 (el) 1999-05-19 2008-03-07 Αφαιρετικη υβριδοποιηση με βαση μικροδιαταξεις

Country Status (18)

Country Link
US (1) US6638717B2 (el)
EP (1) EP1185699B1 (el)
JP (1) JP4714883B2 (el)
KR (1) KR100733930B1 (el)
AT (1) ATE382097T1 (el)
AU (1) AU779901B2 (el)
BR (1) BR0011297A (el)
CA (1) CA2372698C (el)
CY (1) CY1107428T1 (el)
DE (1) DE60037584T2 (el)
DK (1) DK1185699T3 (el)
ES (1) ES2296623T3 (el)
IL (2) IL146547A0 (el)
MX (1) MXPA01011730A (el)
NO (1) NO330813B1 (el)
PT (1) PT1185699E (el)
WO (1) WO2000070098A1 (el)
ZA (1) ZA200109609B (el)

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EP2281888B1 (en) 2004-11-12 2015-01-07 Asuragen, Inc. Methods and compositions involving miRNA and miRNA inhibitor molecules
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US8716190B2 (en) 2007-09-14 2014-05-06 Affymetrix, Inc. Amplification and analysis of selected targets on solid supports
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
US8258111B2 (en) 2008-05-08 2012-09-04 The Johns Hopkins University Compositions and methods related to miRNA modulation of neovascularization or angiogenesis
JP5221248B2 (ja) * 2008-08-26 2013-06-26 株式会社日立ハイテクノロジーズ 高発現遺伝子由来のｃＤＮＡクローンの含有率を低減させたｃＤＮＡライブラリーの作製方法
US20100331204A1 (en) * 2009-02-13 2010-12-30 Jeff Jeddeloh Methods and systems for enrichment of target genomic sequences
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US9644241B2 (en) 2011-09-13 2017-05-09 Interpace Diagnostics, Llc Methods and compositions involving miR-135B for distinguishing pancreatic cancer from benign pancreatic disease
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WO2014144666A2 (en) 2013-03-15 2014-09-18 The University Of Chicago Methods and compositions related to t-cell activity
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EP2971149B1 (en) 2013-03-15 2018-05-09 Baylor Research Institute Ulcerative colitis (uc)-associated colorectal neoplasia markers
KR101516976B1 (ko) * 2013-10-30 2015-05-04 에스케이텔레콤 주식회사 표적 염기 서열 해독에서의 바이어스 제거 방법
CN111566212A (zh) 2017-11-03 2020-08-21 因特尔纳技术有限公司 miRNA分子，等同物，安塔够妙或其来源用于治疗和/或诊断与神经元缺陷相关的病症和/或疾病或用于神经元生成和/或再生
CN113924085A (zh) 2019-04-12 2022-01-11 加利福尼亚大学董事会 用于增加肌肉量和氧化代谢的组合物和方法
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Also Published As

Publication number Publication date
ES2296623T3 (es) 2008-05-01
NO330813B1 (no) 2011-07-25
US20020051970A1 (en) 2002-05-02
ATE382097T1 (de) 2008-01-15
CA2372698C (en) 2010-04-27
JP2002543856A (ja) 2002-12-24
DK1185699T3 (da) 2008-04-28
ZA200109609B (en) 2003-04-30
NO20015635D0 (no) 2001-11-19
IL146547A (en) 2008-04-13
WO2000070098A1 (en) 2000-11-23
PT1185699E (pt) 2008-02-26
EP1185699A1 (en) 2002-03-13
MXPA01011730A (es) 2002-04-24
DE60037584T2 (de) 2009-01-08
US6638717B2 (en) 2003-10-28
DE60037584D1 (de) 2008-02-07
IL146547A0 (en) 2002-07-25
CA2372698A1 (en) 2000-11-23
AU5145500A (en) 2000-12-05
KR100733930B1 (ko) 2007-07-03
NO20015635L (no) 2002-01-15
EP1185699B1 (en) 2007-12-26
JP4714883B2 (ja) 2011-06-29
KR20020008190A (ko) 2002-01-29
BR0011297A (pt) 2002-02-26
AU779901B2 (en) 2005-02-17

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