ATE343586T1 - Verfahren zur verwendung einer universellen bibliothek von peptid-nukleinsäuren zur optimierung der dns-hybridisierung - Google Patents

Verfahren zur verwendung einer universellen bibliothek von peptid-nukleinsäuren zur optimierung der dns-hybridisierung

Info

Publication number
ATE343586T1
ATE343586T1 AT99933646T AT99933646T ATE343586T1 AT E343586 T1 ATE343586 T1 AT E343586T1 AT 99933646 T AT99933646 T AT 99933646T AT 99933646 T AT99933646 T AT 99933646T AT E343586 T1 ATE343586 T1 AT E343586T1
Authority
AT
Austria
Prior art keywords
library
component
sequence
universal
high throughput
Prior art date
Application number
AT99933646T
Other languages
English (en)
Inventor
Soonkap Hahn
Andrew Patron
Roberto Fagnani
Original Assignee
Biocept Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biocept Inc filed Critical Biocept Inc
Application granted granted Critical
Publication of ATE343586T1 publication Critical patent/ATE343586T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
    • C07K14/003—Peptide-nucleic acids (PNAs)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C07K1/047—Simultaneous synthesis of different peptide species; Peptide libraries

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT99933646T 1998-07-09 1999-07-01 Verfahren zur verwendung einer universellen bibliothek von peptid-nukleinsäuren zur optimierung der dns-hybridisierung ATE343586T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US11321198A 1998-07-09 1998-07-09

Publications (1)

Publication Number Publication Date
ATE343586T1 true ATE343586T1 (de) 2006-11-15

Family

ID=22348188

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99933646T ATE343586T1 (de) 1998-07-09 1999-07-01 Verfahren zur verwendung einer universellen bibliothek von peptid-nukleinsäuren zur optimierung der dns-hybridisierung

Country Status (8)

Country Link
US (1) US6433134B1 (de)
EP (1) EP1095054B1 (de)
JP (1) JP2002520008A (de)
KR (1) KR100694914B1 (de)
AT (1) ATE343586T1 (de)
AU (1) AU4965499A (de)
DE (1) DE69933770D1 (de)
WO (1) WO2000002899A1 (de)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1412517T3 (da) * 2001-03-09 2007-03-05 Boston Probes Inc Fremgangsmåder, kits og sammensætninger af kombinationsoligomerer
CA2443060A1 (en) * 2001-04-03 2002-10-17 Biocept, Inc. Methods and gel compositions for encapsulating living cells and organic molecules
US20030108917A1 (en) * 2001-09-01 2003-06-12 Nam Huh Method for manufacturing hydrogel biochip by using star-like polyethylene glycol derivative having epoxy group
EP1442137A4 (de) * 2001-11-07 2005-08-31 Applera Corp Universelle nukleotide für die nukleinsäureanalyse
AU2002351164A1 (en) * 2001-11-26 2003-06-10 Molecular Reflections, Inc. Microscale immobilization of molecules using a hydrogel and methods of use thereof
KR100464261B1 (ko) 2002-01-24 2005-01-03 주식회사 파나진 Ｐｎａ 올리고머를 합성하기 위한 신규한 단량체 및 그의제조방법
US20030175828A1 (en) * 2002-03-15 2003-09-18 Lazar James G. Signal amplification by Hybrid Capture
KR20030084444A (ko) 2002-04-26 2003-11-01 주식회사 파나진 Ｐｎａ 올리고머를 합성하기 위한 신규한 단량체 및 그의제조방법
CA2495895A1 (en) * 2002-09-08 2004-03-18 Applera Corporation Methods, compositions and libraries pertaining pna dimer and pna oligomer synthesis
JP2007516695A (ja) * 2003-06-12 2007-06-28 アプレラ コーポレイション ユニバーサル塩基アナログを含む組合せ核酸塩基オリゴマーならびにこれらを作製する方法および使用する方法
US7211668B2 (en) 2003-07-28 2007-05-01 Panagene, Inc. PNA monomer and precursor
JP2008527979A (ja) * 2005-01-12 2008-07-31 アプレラ コーポレイション 核酸の選択的増幅のための組成物、方法およびキット
US7482353B2 (en) 2005-06-15 2009-01-27 Applied Biosystems Inc. Compositions and methods pertaining to PNA synthons and oligomers comprising a universal base
WO2007041201A2 (en) 2005-10-03 2007-04-12 Applera Corporation Compositions, methods, and kits for amplifying nucleic acids
US20070172842A1 (en) * 2006-01-25 2007-07-26 Andreas Braun Methods for isolating and identifying novel target-specific structuremers for use in the biological sciences
EP2739587B1 (de) 2011-08-01 2020-05-27 Denovo Sciences Zellerfassungssystem
US9174216B2 (en) 2013-03-13 2015-11-03 DeNovo Science, Inc. System for capturing and analyzing cells
US9404864B2 (en) 2013-03-13 2016-08-02 Denovo Sciences, Inc. System for imaging captured cells
US10466160B2 (en) 2011-08-01 2019-11-05 Celsee Diagnostics, Inc. System and method for retrieving and analyzing particles
US9606102B2 (en) 2013-01-26 2017-03-28 Denovo Sciences, Inc. System and method for capturing and analyzing cells
US9707562B2 (en) 2013-03-13 2017-07-18 Denovo Sciences, Inc. System for capturing and analyzing cells
US9856535B2 (en) 2013-05-31 2018-01-02 Denovo Sciences, Inc. System for isolating cells
US10391490B2 (en) 2013-05-31 2019-08-27 Celsee Diagnostics, Inc. System and method for isolating and analyzing cells
CN105813721B (zh) 2013-10-16 2019-11-05 不列颠哥伦比亚大学 用于以小体积配制颗粒的装置
EP3797860A1 (de) 2016-01-06 2021-03-31 The University of British Columbia Verzweigte mischer sowie verfahren zu deren verwendung und herstellung
CN106117153B (zh) * 2016-07-05 2018-11-23 淮阴师范学院 2-哌嗪酮的制备方法
BR112019018051A2 (pt) * 2017-04-12 2020-04-07 Hoffmann La Roche método para produzir um composto, composto e substância
US10391493B2 (en) 2017-08-29 2019-08-27 Celsee Diagnostics, Inc. System and method for isolating and analyzing cells
US11419947B2 (en) 2017-10-30 2022-08-23 Massachusetts Institute Of Technology Layer-by-layer nanoparticles for cytokine therapy in cancer treatment
US10633693B1 (en) 2019-04-16 2020-04-28 Celsee Diagnostics, Inc. System and method for leakage control in a particle capture system
CN114072490A (zh) 2019-05-07 2022-02-18 伯乐实验室有限公司 用于自动化的单细胞加工的系统和方法
US11273439B2 (en) 2019-05-07 2022-03-15 Bio-Rad Laboratories, Inc. System and method for target material retrieval from microwells
US12018315B2 (en) 2019-05-30 2024-06-25 Massachusetts Institute Of Technology Peptide nucleic acid functionalized hydrogel microneedles for sampling and detection of interstitial fluid nucleic acids
CA3143241A1 (en) 2019-06-14 2020-12-17 Bio-Rad Laboratories, Inc. System and method for automated single cell processing and analyses
CN110511973B (zh) * 2019-07-16 2021-05-07 杭州原合生物科技有限公司 一种用于核酸原位合成的固相载体及其制备方法
KR102403904B1 (ko) * 2019-12-24 2022-06-02 주식회사 시선바이오머티리얼스 용액공정상 pna 올리고머의 제조방법
US11504719B2 (en) 2020-03-12 2022-11-22 Bio-Rad Laboratories, Inc. System and method for receiving and delivering a fluid for sample processing
KR20220057138A (ko) 2020-10-29 2022-05-09 엘지전자 주식회사 청소기
KR20240125170A (ko) 2023-02-10 2024-08-19 엘지전자 주식회사 청소기 및 청소기 제어 방법

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE171185T1 (de) 1985-03-15 1998-10-15 Antivirals Inc Immunotestmittel für polynukleotid und verfahren
US5714331A (en) 1991-05-24 1998-02-03 Buchardt, Deceased; Ole Peptide nucleic acids having enhanced binding affinity, sequence specificity and solubility
US5539082A (en) 1993-04-26 1996-07-23 Nielsen; Peter E. Peptide nucleic acids
US5641625A (en) 1992-05-22 1997-06-24 Isis Pharmaceuticals, Inc. Cleaving double-stranded DNA with peptide nucleic acids
DK51092D0 (da) 1991-05-24 1992-04-15 Ole Buchardt Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf
US5766855A (en) 1991-05-24 1998-06-16 Buchardt, Deceased; Ole Peptide nucleic acids having enhanced binding affinity and sequence specificity
US5700922A (en) * 1991-12-24 1997-12-23 Isis Pharmaceuticals, Inc. PNA-DNA-PNA chimeric macromolecules
US5438131A (en) 1992-09-16 1995-08-01 Bergstrom; Donald E. 3-nitropyrrole nucleoside
US5539083A (en) * 1994-02-23 1996-07-23 Isis Pharmaceuticals, Inc. Peptide nucleic acid combinatorial libraries and improved methods of synthesis
US5738996A (en) 1994-06-15 1998-04-14 Pence, Inc. Combinational library composition and method
DE69527857T2 (de) 1994-10-06 2003-05-28 Buchardt, Dorte Peptid-nukleinsäure-konjugate
AU4159396A (en) 1994-11-14 1996-06-06 Chiron Corporation Synthesis of peptide nucleic acids (pnas) and analogues via submonomer approach
WO1996020212A2 (en) * 1994-12-28 1996-07-04 Buchardt, Dorte Peptide nucleic acid incorporating a chiral backbone
DE19532553A1 (de) 1995-09-04 1997-03-06 Hoechst Ag Verfahren zur Herstellung substituierter N-Ethyl-Glycinderivate
DE19627898A1 (de) 1996-07-11 1998-01-15 Hoechst Ag Festphasensynthese von Oligonucleotiden

Also Published As

Publication number Publication date
WO2000002899A9 (en) 2000-07-27
AU4965499A (en) 2000-02-01
KR20010074672A (ko) 2001-08-08
EP1095054B1 (de) 2006-10-25
US6433134B1 (en) 2002-08-13
EP1095054A1 (de) 2001-05-02
KR100694914B1 (ko) 2007-03-14
JP2002520008A (ja) 2002-07-09
DE69933770D1 (de) 2006-12-07
WO2000002899A1 (en) 2000-01-20

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