CS231161B2 - Method of separating of nucleid acid mixtures - Google Patents

Method of separating of nucleid acid mixtures Download PDF

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Publication number
CS231161B2
CS231161B2 CS781289A CS128978A CS231161B2 CS 231161 B2 CS231161 B2 CS 231161B2 CS 781289 A CS781289 A CS 781289A CS 128978 A CS128978 A CS 128978A CS 231161 B2 CS231161 B2 CS 231161B2
Authority
CS
Czechoslovakia
Prior art keywords
nucleic acids
anion
group
stranded
polymeric
Prior art date
Application number
CS781289A
Other languages
Czech (cs)
English (en)
Other versions
CS7801289A2 (en
Inventor
Werner Mueller
Hans Buenemann
Original Assignee
Boehringer Mannheim Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh
Priority to CS801880A priority Critical patent/CS242863B2/cs
Publication of CS7801289A2 publication Critical patent/CS7801289A2/cs
Publication of CS231161B2 publication Critical patent/CS231161B2/cs

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
    • B01J20/3255—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such comprising a cyclic structure containing at least one of the heteroatoms nitrogen, oxygen or sulfur, e.g. heterocyclic or heteroaromatic structures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3214—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating
    • B01J20/3217—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond
    • B01J20/3219—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond involving a particular spacer or linking group, e.g. for attaching an active group
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
    • B01J20/3253—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such comprising a cyclic structure not containing any of the heteroatoms nitrogen, oxygen or sulfur, e.g. aromatic structures
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D219/00—Heterocyclic compounds containing acridine or hydrogenated acridine ring systems
    • C07D219/04—Heterocyclic compounds containing acridine or hydrogenated acridine ring systems with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to carbon atoms of the ring system
    • C07D219/08—Nitrogen atoms
    • C07D219/10—Nitrogen atoms attached in position 9
    • C07D219/12—Amino-alkylamino radicals attached in position 9
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D221/00—Heterocyclic compounds containing six-membered rings having one nitrogen atom as the only ring hetero atom, not provided for by groups C07D211/00 - C07D219/00
    • C07D221/02—Heterocyclic compounds containing six-membered rings having one nitrogen atom as the only ring hetero atom, not provided for by groups C07D211/00 - C07D219/00 condensed with carbocyclic rings or ring systems
    • C07D221/04—Ortho- or peri-condensed ring systems
    • C07D221/06—Ring systems of three rings
    • C07D221/10—Aza-phenanthrenes
    • C07D221/12—Phenanthridines
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F265/00—Macromolecular compounds obtained by polymerising monomers on to polymers of unsaturated monocarboxylic acids or derivatives thereof as defined in group C08F20/00
    • C08F265/10—Macromolecular compounds obtained by polymerising monomers on to polymers of unsaturated monocarboxylic acids or derivatives thereof as defined in group C08F20/00 on to polymers of amides or imides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
CS781289A 1977-03-02 1978-03-01 Method of separating of nucleid acid mixtures CS231161B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CS801880A CS242863B2 (cs) 1977-03-02 1978-03-01 Způsob výroby nových sloučenin typu akryloylaminobarviv

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE2709094A DE2709094C2 (de) 1977-03-02 1977-03-02 Adsorbens für die affinitätsspezifische Trennung von Nukleinsäuren, Verfahren zu seiner Herstellung und seine Verwendung

Publications (2)

Publication Number Publication Date
CS7801289A2 CS7801289A2 (en) 1984-02-13
CS231161B2 true CS231161B2 (en) 1984-10-15

Family

ID=6002605

Family Applications (2)

Application Number Title Priority Date Filing Date
CS781289A CS231169B2 (en) 1977-03-02 1978-03-01 Processing method of adsorbing agent for affinitive separation of nucleic acids
CS781289A CS231161B2 (en) 1977-03-02 1978-03-01 Method of separating of nucleid acid mixtures

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CS781289A CS231169B2 (en) 1977-03-02 1978-03-01 Processing method of adsorbing agent for affinitive separation of nucleic acids

Country Status (14)

Country Link
US (2) US4335226A (de)
JP (1) JPS53131294A (de)
AT (1) AT364734B (de)
CA (1) CA1122208A (de)
CH (1) CH651222A5 (de)
CS (2) CS231169B2 (de)
DE (1) DE2709094C2 (de)
DK (1) DK150536C (de)
FR (1) FR2416721A1 (de)
GB (2) GB1597891A (de)
HU (1) HU182462B (de)
IT (1) IT1095439B (de)
NL (1) NL7802022A (de)
SE (1) SE7802249L (de)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2534486B1 (fr) * 1982-10-15 1987-11-20 Commissariat Energie Atomique Support particulaire greffe en surface, son procede de preparation et adsorbants pour chromatographie d'affinite incorporant ce support, ainsi que leur utilisation, notamment en biologie
US4665184A (en) * 1983-10-12 1987-05-12 California Institute Of Technology Bifunctional molecules having a DNA intercalator or DNA groove binder linked to ethylene diamine tetraacetic acid
DE3407814A1 (de) * 1984-03-02 1985-09-12 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen Phasentraeger fuer die verteilungschromatographie von makromolekuelen, verfahren zu ihrer herstellung und ihre verwendung
US5368737A (en) * 1984-03-29 1994-11-29 Daicel Chemical Industries, Ltd. Separation agent comprising acyl-or carbamoyl-substituted polysaccharide
US5562614A (en) * 1993-11-22 1996-10-08 Advanced Cardiovascular Systems, Inc. Programmable manifold system for automatic fluid delivery
JPS60226829A (ja) * 1984-03-29 1985-11-12 Daicel Chem Ind Ltd 多糖誘導体より成る分離剤
US5229002A (en) * 1984-03-29 1993-07-20 Daicel Chemical Industries, Ltd. Separation agent comprising acyl- or carbamoyl-substituted polysaccharide
US5489387A (en) * 1984-03-29 1996-02-06 Daicel Chemical Industries, Ltd. Separation agent comprising acyl- or carbamoyl-substituted polysaccharide
DE3619303A1 (de) * 1986-06-07 1987-12-10 Merck Patent Gmbh Optisch aktive adsorbentien
DE3811042A1 (de) * 1988-03-31 1989-10-19 Merck Patent Gmbh Ionenaustauscher
US5342785A (en) * 1988-07-05 1994-08-30 Tanabe Seiyaku Co., Ltd. Method for determining pyrogen content
US4957620A (en) * 1988-11-15 1990-09-18 Hoechst Celanese Corporation Liquid chromatography using microporous hollow fibers
JP2925753B2 (ja) * 1990-02-23 1999-07-28 ダイセル化学工業株式会社 光学異性体の分離方法
EP0708919B1 (de) * 1993-07-16 1998-09-16 MERCK PATENT GmbH Trennmaterialien für die hydrophobe chromatographie
DE4333674A1 (de) * 1993-10-02 1995-04-06 Merck Patent Gmbh Nukleotidhaltiges Sorbens für die Affinitätschromatographie
DE4333821A1 (de) * 1993-10-04 1995-04-06 Merck Patent Gmbh Ionenaustauscher
DE4334353A1 (de) * 1993-10-08 1995-04-13 Merck Patent Gmbh Verfahren und Träger für die Gelpermeationschromatographie
JP3249408B2 (ja) 1996-10-25 2002-01-21 昭和シェル石油株式会社 薄膜太陽電池の薄膜光吸収層の製造方法及び製造装置
US7214410B2 (en) * 2002-10-04 2007-05-08 Shipley Company, L.L.C. Process for selecting solvents for forming films of ferroelectric polymers
RU2257200C1 (ru) * 2004-05-12 2005-07-27 Кутушов Михаил Владимирович Лекарственное средство
JP4933610B2 (ja) * 2006-04-18 2012-05-16 ダウ・コーニング・コーポレイション 銅インジウム二セレン化物をベースとする光起電デバイス及びその光起電デバイスを作製する方法
ATE441942T1 (de) * 2006-04-18 2009-09-15 Dow Corning Fotovoltaische anordnung auf kupfer-indium- diselenidbasis und herstellungsverfahren dafür
JP5253380B2 (ja) * 2006-04-18 2013-07-31 ダウ・コーニング・コーポレイション テルル化カドミウムベース光起電力デバイスおよびその調製方法
JP5908840B2 (ja) * 2010-09-22 2016-04-26 株式会社カネカ 核酸の検出方法及びデバイス、キット
CA2811369C (en) * 2010-09-29 2019-09-03 Intervet International B.V. N-heteroaryl compounds
TWI538235B (zh) 2011-04-19 2016-06-11 弗里松股份有限公司 薄膜光伏打裝置及製造方法
US20140196894A1 (en) * 2013-01-15 2014-07-17 University Of Kansas Fluorescent tags for detection of swellable polymers
US9958364B2 (en) * 2013-10-03 2018-05-01 3M Innovative Properties Company Ligand functionalized substrates with enhanced binding capacity

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3640983A (en) * 1966-11-09 1972-02-08 Shojiro Horiguchi Method of making coupler-bonded-polymers and chromogen-bonded-polymers nd polymers made thereby
US3983001A (en) * 1972-05-10 1976-09-28 Ceskoslovenska Akademie Ved Isolation of biologically active compounds by affinity chromatography
NL7411909A (nl) * 1973-09-10 1975-03-12 Research Corp Werkwijze voor het bereiden van een samenstel- ling op basis van een kleurstof, werkwijze voor het isoleren van albumine.
US4017476A (en) * 1974-03-15 1977-04-12 Mobil Oil Corporation Preparation of finely divided styrene-divinylbenzene organic pigments
FR2268022B1 (de) * 1974-04-22 1977-06-24 Rhone Poulenc Ind
US4046750A (en) * 1974-09-30 1977-09-06 California Institute Of Technology Ionene modified small polymeric beads
LU73094A1 (de) * 1975-07-29 1977-03-24
US4035316A (en) * 1975-11-24 1977-07-12 California Institute Of Technology Cell specific, variable density, polymer microspheres
US4213860A (en) * 1976-06-30 1980-07-22 Board of Regents, State of Florida for and on behalf of the University of Florida Affinity chromatography and substrate useful therefor
US4194877A (en) * 1977-11-28 1980-03-25 United States Of America Dye-containing polymer composition

Also Published As

Publication number Publication date
CS231169B2 (en) 1984-10-15
DE2709094C2 (de) 1984-11-22
DK150536C (da) 1987-10-19
FR2416721A1 (fr) 1979-09-07
GB1597892A (en) 1981-09-16
CS188180A2 (en) 1984-02-13
JPS53131294A (en) 1978-11-15
DK150536B (da) 1987-03-23
HU182462B (en) 1984-01-30
CS7801289A2 (en) 1984-02-13
JPS6219892B2 (de) 1987-05-01
NL7802022A (nl) 1978-09-05
CA1122208A (en) 1982-04-20
DK90878A (da) 1978-09-03
IT1095439B (it) 1985-08-10
CH651222A5 (de) 1985-09-13
ATA146078A (de) 1981-04-15
GB1597891A (en) 1981-09-16
SE7802249L (sv) 1978-09-03
DE2709094A1 (de) 1978-09-07
IT7820640A0 (it) 1978-02-24
US4394487A (en) 1983-07-19
AT364734B (de) 1981-11-10
FR2416721B1 (de) 1983-01-21
US4335226A (en) 1982-06-15

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