CN209845910U - Three-dimensional rooting and seedling strengthening device for tissue culture seedlings of hybrid paper mulberry - Google Patents
Three-dimensional rooting and seedling strengthening device for tissue culture seedlings of hybrid paper mulberry Download PDFInfo
- Publication number
- CN209845910U CN209845910U CN201920445617.2U CN201920445617U CN209845910U CN 209845910 U CN209845910 U CN 209845910U CN 201920445617 U CN201920445617 U CN 201920445617U CN 209845910 U CN209845910 U CN 209845910U
- Authority
- CN
- China
- Prior art keywords
- layer
- seedling
- paper mulberry
- tissue culture
- rock wool
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
Abstract
The utility model belongs to the technical field of paper mulberry tissue culture, a three-dimensional strong seedling device of taking root of hybridization paper mulberry tissue culture seedling is disclosed, include: a water storage tank built in the greenhouse; a Dutch net is laid above the water storage tank; taking a Dutch net as a support, and paving an agricultural rock wool layer with spaced holes on the Dutch net; the periphery of the three-layer three-dimensional culture rack is supported by a fixed frame, three layers of three-dimensional culture racks with the interval of 40cm are built, each layer takes a dutch net as a support and rock wool with the interval of 5cm and the thickness of 2cm as a surface, and each layer is provided with a micro-spray head; a sugar-free nutrient solution layer is filled in the water storage tank; the sugar-free nutrient solution layer is pumped out by a water pump to the rock wool layer, and the missed sugar-free nutrient solution layer flows into the water storage tank through the saturated rock wool layer. The utility model lays a foundation for cultivating strong and high-quality hybrid paper mulberry seedlings and shortening the seedling period; the utility model provides a hybridization paper mulberry tissue culture seedling take root badly, tame the not high scheduling problem of survival rate.
Description
Technical Field
The utility model belongs to the technical field of paper mulberry tissue culture, especially, relate to a three-dimensional strong seedling device of taking root of hybridization paper mulberry tissue culture seedling.
Background
Currently, the current state of the art commonly used in the industry is such that:
china is one of the biggest breeding producing countries in the world, and is also a country with large demand for feed raw materials, particularly high-protein raw materials in the world. Has deep dependence on the international raw material market, inhibits the development of feed industry, and has long-term structural contradiction due to the shortage of feed raw materials. How to solve the problem of feed raw materials is the key to promote the development of the breeding industry. Solves the bottleneck problem that the high protein feed raw material in China becomes the development of animal husbandry. A new feed variety hybrid paper mulberry rich in protein is cultivated in a plant key laboratory of Chinese academy of sciences by combining hybrid breeding with modern biotechnology, and is also called as white mulberry because the tree has the characteristics of high protein content and amino acid content, fast growth, high yield, multiple resistance, cutting resistance, no diseases and insect pests and the like. Because the ecological poverty-relieving fertilizer has important economic and ecological values in the aspects of feed, paper making, ecological greening and the like, a large number of plants are planted in more than 20 provinces in China at present, so that the ecological poverty-relieving fertilizer can help farmers to lose poverty and become rich and can improve the ecological environment of poverty-relieving areas, and the ecological poverty-relieving fertilizer is listed as one of ten precise poverty-relieving projects in 2015. Poverty alleviation engineering of paper mulberry (white mulberry) is led by poverty alleviation of state hospitals, various industry departments are coordinated, a 'poverty alleviation of hybrid paper mulberry industry' project group is established, and the state hospitals focus on implementing the poverty alleviation of the white mulberry 'forest-feed-livestock' integrated livestock industry in poverty alleviation areas of China. As an important agricultural and industrial raw material, paper mulberry not only has wide development prospect in the fields of papermaking and feed production, but also is increasingly sought by people as the identity of greening tree species. The research on the paper mulberry is still in the initial stage of research in China, the paper mulberry on the market is still concentrated into most wild tree species, and the project of large-scale artificial paper mulberry breeding in China is rarely reported in a large scale. In recent years, most of related researches on paper mulberry trees focus on measurement and property research of paper mulberry active substances, and at the present stage, the breeding mode of paper mulberry seedlings is mainly traditional cutting propagation, but the cutting propagation has obvious defects, such as underdeveloped paper mulberry root systems, inconsistent growth vigor of paper mulberry trees at the later stage, inconvenience for mechanical management operation, easiness in hollowing of trunks and the like. Therefore, the tissue culture and breeding of the broussonetia papyrifera seedlings by using an efficient and feasible biotechnology means to realize the large-scale production of the broussonetia papyrifera seedlings is the mainstream trend of the current broussonetia papyrifera cultivation.
However, the paper mulberry tissue culture technology is easy to cause poor rooting in the rooting link, the roots are fragile and easy to break, callus is easy to generate on the roots, seedlings are easy to rot in the domestication stage, the difficulty of the later domestication technology is high, and the development of paper mulberry tissue culture large-scale production is always restricted.
In summary, the problems of the prior art are as follows:
(1) in the prior art, the broussonetia papyrifera seedlings bred by seeding have long growth period, and the later generations cannot keep the excellent characters of the female parent; the labor consumption of the paper mulberry cutting seedlings is high, and in the later growth period, the root systems are not as good as those of the seedling seedlings and the tissue culture seedlings, the tree vigor is weak, and the trunk is easy to be hollow; the tissue culture technique of paper mulberry can solve the problems of rapid propagation and maintaining of excellent characters of female parent. However, poor rooting is easy to occur in the rooting link, the roots are fragile and easy to break, callus generated on the roots is easy to rot in the domestication stage, the problems improve the seedling domestication difficulty of the later-stage tissue culture seedlings and restrict the development of large-scale production of the tissue culture seedlings of the paper mulberry.
(2) The production of the tissue culture seedling of the paper mulberry is carried out through the links of detoxification, dedifferentiation, callus, rooting, seedling hardening and the like, the seedling culture period is longer, and the cost is higher; in addition, in the tissue culture process of the paper mulberry, the paper mulberry is not easy to root and has poor rooting, and roots are easy to rot and difficult to survive during domestication.
(3) The tissue culture seedling is easy to be polluted, in the process of culture, due to reasons of overhigh concentration ratio of hormone and the like, the culture material can also be mutated, browned, yellowed, vitrified and dead, or the proliferation rate is too low or high, and the tissue culture seedling takes root abnormally or does not take root; the tissue culture seedling grows excessively or is thin and weak, and the like, so that the death rate of the bottle seedling is high.
The difficulty and significance for solving the technical problems are as follows:
the rapid propagation technology combining the tissue culture of the hybrid paper mulberry and the sugar-free rooting technology of rock wool still has a lot of problems, and how to select the optimal conditions of each link; judging whether the plant grows normally in the tissue culture according to the standard; how to balance the compositions of various mineral elements and hormones and overcome various adverse factors and the like all of which can increase the technical difficulty of the experiment and influence the rooting effect when the induced culture is developed towards the expected direction.
The technical problem can be solved, the time from rooting to seedling hardening can be really shortened, the seedling hardening survival rate is improved, and the economic cost and the labor cost of tissue culture paper mulberry trees are effectively reduced.
SUMMERY OF THE UTILITY MODEL
Problem to prior art existence, the utility model provides a three-dimensional strong seedling device of taking root of hybridization paper mulberry tissue culture seedling.
The utility model discloses a realize like this, utilize the rock wool to make the matrix and carry out soilless three-dimensional culture, make full use of rock wool's gas permeability and water-retaining property, let the quick good root of paper mulberry tissue culture seedling, and the 1/2WPM +0.5mg/L NAA +0.1mg/L IBA +0.15mg/L ABT of nutrient solution improvement for not adding agar and cane sugar, provide the required nutrition of root growth, the sugar-free nutrient solution has reduced the growth of miscellaneous fungus, it is as an organic whole with traditional link of taking root and the link of hardening off seedlings, thereby the quality of taking root and the survival rate of domesticating of tissue culture seedling have been improved.
A three-dimensional rooting and seedling strengthening device for tissue culture seedlings of hybrid paper mulberry comprises:
a water storage tank built in the greenhouse; a Dutch net is laid above the water storage tank; the Dutch net is a support, and an agricultural rock wool layer with spaced holes is laid on the Dutch net; the periphery of the three-layer three-dimensional culture rack is supported by a fixed frame, three layers of three-dimensional culture racks with the interval of 40cm are built, a Dutch net is used as a support for each layer, rock wool with the interval of 5cm and the thickness of 2cm is used as a surface for each layer, and a micro-spray head is arranged on each layer.
A sugar-free nutrient solution layer is filled in the water storage tank;
the sugar-free nutrient solution layer is pumped out by a water pump to the rock wool layer, and the missed sugar-free nutrient solution flows into the water storage tank through the saturated rock wool layer.
Furthermore, the water pump controls the micro-spray time and times of the micro-spray through an integrated mechanical timer.
Further, the water storage tank is a cement tank with a brick-concrete structure.
Further, the sugar-free nutrient solution layer is provided for the rock wool layer by a water pump, a PVC pipe and a micro-nozzle.
Furthermore, the bottom of the water storage pool for rooting and seedling raising is a cuboid sealing device.
To sum up, the utility model discloses an advantage and positive effect do:
the utility model discloses with the basal portion that the strong seedling nutrient solution of taking root was organized the seedling by little shower nozzle spraying rock wool layer and paper mulberry, impel to let the seedling grow up fast, take root 25-35 from original, shorten to full root system in 15-20 days.
The utility model provides a hybridization paper mulberry tissue culture seedling take root bad domestication survival rate scheduling problem not, take root the link with the synthetic an organic whole of hardening off the link of original hybridization paper mulberry tissue culture seedling and change into sugar-free nutrient solution rock wool to the rock wool cultivates the biggest root nutrition that provides, the growth of the miscellaneous fungus of sugar-free nutrient solution maximum reduction, thereby improve hybridization paper mulberry tissue culture seedling quality of taking root and domestication survival rate greatly, establish the basis for cultivating strong high-quality hybridization paper mulberry seedling.
The utility model discloses a value of hybridization paper mulberry:
with the continuous and rapid increase of global population and the continuous and rapid progress of urbanization, the agricultural available arable land area is reduced year by year, the problem of poor food resources is increasingly aggravated, and the problem of environmental pollution is urgent. Therefore, the popularization of the broussonetia papyrifera planting has important significance for improving agricultural development and constructing good ecological environment. Meanwhile, the application of the paper mulberry leaves in feed production can save a large amount of grains and improve the current situation of grain shortage. The novel hybrid broussonetia papyrifera species researched by adopting the space biotechnology has extremely high content of crude protein in leaves, and simultaneously, nutrient substances in the leaves are extremely uniformly distributed, so that the novel hybrid broussonetia papyrifera species can be prepared into broussonetia papyrifera nutrient powder, and grain products, health care products and the like which are prepared by mixing the novel hybrid broussonetia papyrifera nutrient powder with flour, bean flour and the like. In addition, the broussonetia papyrifera is used for greening and transforming barren lands such as barren hills and hills, the ecological environment is improved, the water-soil ratio is balanced, the benefits of farmers on the barren lands are properly improved and protected, and the pace of enabling the farmers to enter the comfortable life is accelerated. Therefore, the market value of the paper mulberry is immeasurable, and a huge development space is still left.
The utility model discloses accord with the theory of "replacing grain with the tree": the broussonetia papyrifera leaf feed replaces the original grain feed to feed slaughtered livestock, so that the broussonetia papyrifera leaf feed is fast in growth and good in meat quality, considerable economic benefits are achieved, and the development prospect of the broussonetia papyrifera leaf in the field of feed production is very wide.
The utility model discloses the medicine health care of paper mulberry is worth: fruits, roots and seeds of the paper mulberry can be used as Chinese medicinal materials, so that the paper mulberry not only has the effects of strengthening muscles and bones, but also can tonify kidney and promote urination; secondly, the sap of the paper mulberry also has obvious curative effect on treating skin diseases.
The utility model discloses the industrial value of paper mulberry: the paper making tree has extremely high cellulose content, and the tensile length of the bast fiber is beyond the reach of other traditional paper making tree species. The pulp yield of paper making by paper making and pulping by paper mulberry is also named as the top grass in other tree species, and the pulp whiteness is also at an advanced level. The paper mulberry has lignin content of about 20-30%, average phloem fiber length of 7.5mm, pulp yield twice higher than that of the traditional paper making poplar and eucalyptus, and raw material consumption per unit weight for pulping is only one fifth of that of the poplar, so that the paper mulberry has excellent environmental protection. Therefore, paper mulberry pulp is often used as a raw material for making high-grade paper such as rice paper, and paper mulberry is also the preferred raw material for producing density boards and high-strength corrugated paper.
The utility model discloses the urban afforestation of paper mulberry is worth: as the paper mulberry not only has excellent and stable saline-alkali resistance, but also has rather stubborn viability under severe stress conditions. Many research practices prove that the level of growth and breeding of the paper mulberry in moderate saline-alkali soil is much higher than the level of breeding requirements of greening tree species, and the paper mulberry is suitable for cultivation and greening in saline-alkali soil. The paper mulberry not only has the capability of growing in severe and extreme soil environments, such as high saline-alkali soil, drought soil and the like, but also has outstanding performance in the aspect of disease and insect pest resistance, and the possibility of being infected by the disease and insect pest is reduced due to the short felling period. In a word, the broussonetia papyrifera has extremely high popularization value in the aspects of greening barren land, controlling water and soil loss and repairing the ecological environment, and is suitable for large-area planting.
Drawings
FIG. 1 is a drawing of an apparatus for three-dimensional rooting and seedling strengthening of tissue culture hybrid paper mulberry seedlings provided by the embodiment of the utility model.
Fig. 2 is a schematic view of a greenhouse provided by the embodiment of the present invention.
Fig. 3 is a schematic view of a water storage tank according to an embodiment of the present invention.
Fig. 4 is a schematic view of the dutch net provided by the embodiment of the present invention.
In the figure: 1. a greenhouse; 2. a water storage tank; 3. a Dutch net; 4. a rock wool layer; 5. a micro-shower nozzle; 6. a sugar-free nutrient solution layer; 7. a water pump; 8. PVC pipes; 9. cuboid sealing device.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
In the prior art, the paper mulberry tissue culture technology is a bottleneck in the rooting link all the time, the rooting is poor, the root is fragile and easy to break, the callus is easy to generate on the root, the seedling is easy to rot in the domestication stage, the difficulty of the later domestication technology is high, and the development of the paper mulberry tissue culture large-scale production is always restricted; the paper mulberry tissue culture method has the advantages that the paper mulberry tissue culture method is not easy to root, poor in rooting and difficult to survive in domestication.
The utility model is further described below with reference to specific analysis.
As shown in fig. 1-4, the embodiment of the utility model provides a sugarless nutrient solution rock wool of paper mulberry tissue culture seedling three-dimensional device of strengthening seedlings that takes root of hybridization includes:
a water storage tank 2 built in the greenhouse 1; a Dutch net 3 is laid above the water storage tank 2; the Dutch net is a support, and an agricultural rock wool layer 4 with spaced holes is laid on the Dutch net;
micro spray heads 5 are arranged at the middle position inside the water storage tank 2 at intervals, and are arranged in the middle of the water storage tank;
a sugar-free nutrient solution layer 6 is filled in the water storage tank;
the sugar-free nutrient solution layer 6 is pumped out by a water pump to the rock wool layer, and the missed sugar-free nutrient solution flows into the water storage tank through the saturated rock wool layer.
The water pump 7 controls the micro-spray time and times of the micro-spray through an integrated mechanical timer.
The water storage tank is a cement tank with a brick-concrete structure.
The sugar-free nutrient solution layer 6 is provided for the rock wool layer by the water pump 7, the PVC pipe 8 and the micro-spray head 5.
The bottom of the water storage tank is a cuboid sealing device 9.
The utility model is further described below in connection with the principle analysis.
In the three-dimensional rooting and seedling strengthening device for the tissue culture seedlings of the hybrid paper mulberry provided by the embodiment of the utility model,
the tissue culture seedling of the hybrid paper mulberry is rooted and strengthened on the rock wool layer, and the improved sugar-free WPM liquid culture medium is used as nutrition.
The tissue culture seedling of the hybrid paper mulberry is rooted and strengthened on a rock wool layer, the tissue culture seedling is fixed by taking rock wool as a matrix, and the base part of the seedling is arranged in a hole with the diameter of 0.5cm of the rock wool layer.
The rooting and seedling strengthening nutrient solution for raising the seedlings is sprayed by a water pump, a PVC pipe and a micro-nozzle and provided for the rock wool layer and the seedling base part to absorb, so that the rapid rooting and the strengthening are promoted.
The rooting and seedling strengthening nutrient solution is an improved sugar-free WPM nutrient solution, and specifically is 1/2WPM, 0.5mg/L NAA, 0.1mg/L IBA and 0.15mg/L ABT without agar and sucrose.
The roots of the tissue culture seedlings are fully contacted with rock wool, air and nutrient solution, so that the tissue culture seedlings can rapidly root and grow.
The upper part of the culture pond for rooting and seedling raising is composed of a rock wool layer with thickness, the bottom of the culture pond is a cuboid sealing device, and a micro-spray nozzle is arranged in the culture pond and can spray.
The method specifically comprises the following steps:
1) manufacturing a culture pond: a water storage pool with the length of 10m and the height of 20cm is built in the greenhouse, and the water storage pool is a cement pool with a brick-concrete structure. A Dutch net (the specification of the Dutch net is 10cm multiplied by 10cm, the diameter of 0.5cm) is laid on the water pool, the Dutch net is taken as a support, and agricultural rock wool with the thickness of 5cm and the holes with the interval of 5cm (the hole diameter of 0.5cm) is laid on the water pool. The periphery of the three-layer three-dimensional culture rack is supported by a fixed frame, three layers of three-dimensional culture racks with the interval of 40cm are built, each layer takes a dutch net as a support and rock wool with the interval of 5cm and the thickness of 2cm as a surface, and each layer is provided with a gardening micro-nozzle with the interval of 60 cm. Nutrient solution is pumped out by a water pump (220V 800W three-impeller water pump) from the water storage tank and is sprayed to the rock wool layer, and redundant nutrient solution flows into the water storage tank through saturated rock wool to continue circulation. The water pump controls the micro-spraying time and times through a mechanical timer.
2) Taking out the tissue culture seedlings of the non-rooted hybrid broussonetia papyrifera from the culture medium by using tweezers, washing the seedlings clean by flowing water, soaking the seedlings for 5 minutes by using three thousandth of carbendazim solution, taking out and airing the seedlings for later use.
3) And inserting the sterilized seedlings on a prepared rock wool seedling raising module at the interval of 5cm, wherein the base parts of the seedlings are placed in rock wool holes.
4) The nutrient solution is sprayed once every 30 minutes for 1 minute to ensure the moisture and nutrition of the rock wool and the seedlings (the nutrient solution is supplied from the pool by a water pump).
5) Keeping the illumination of the seedling at 2000-28 ℃ and the relative humidity at 60%, keeping the rock wool undried by the nutrient solution, and growing clustered root buds after normal growth for 15-20 days for seedling transplantation.
The application of the invention will be further described with reference to specific embodiments.
Manufacturing a culture box body:
the water pool is a cement pool with a brick-concrete structure. A Dutch net (the specification of the Dutch net is 10cm multiplied by 10cm, the diameter of 0.5cm) is laid on the water pool, the Dutch net is taken as a support, and agricultural rock wool with the thickness of 5cm and the holes with the interval of 5cm (the hole diameter of 0.5cm) is laid on the water pool. The periphery of the three-layer three-dimensional culture rack is supported by a fixed frame, three layers of three-dimensional culture racks with the interval of 40cm are built, a Dutch net is used as a support for each layer, rock wool with the interval of 5cm and the thickness of 2cm is used as a surface, and gardening micro-nozzles are installed on each layer with the interval of 60 cm. Nutrient solution is pumped out by a water pump (220V 800W three-impeller water pump) from the water storage tank and is sprayed to the rock wool layer, and redundant nutrient solution flows into the water storage tank through saturated rock wool to continue circulation. The water pump controls the micro-spraying time and times through a mechanical timer.
Preparing a nutrient solution:
nutrient solution formula (pH 5.6-5.8):
subculturing the tissue culture for 25-35 days with the hybrid broussonetia papyrifera with 3-5 leaves and 5-7cm long, taking out from a differentiation culture medium, cleaning, sterilizing with 1000 times of carbendazim and 800 times of azoxystrobin for 5 minutes, and air drying to remove water on the surface for later use. The seedlings are inserted into the seedling holes of the rock wool layer, and the base parts are placed in the rock wool layer, so that the base parts of the seedlings can be completely contacted with air and micro-spraying nutrient solution. The electronic timer is used for controlling the water pump to work, and the water pump is sprayed at the frequency of 1 minute every time for 30 minutes, so that air and nutrition are kept to be supplied to the roots at the same time, and rapid rooting and strengthening are promoted.
Keeping the temperature of the seedling raising box at 26-28 ℃, the relative humidity at more than 65%, and enabling the light of the leaf surface of the seedling to erupt and take roots in the environment of 2000-3000lx within 15-20 days. And (4) carrying out underground planting on the broussonetia papyrifera seedlings with roots and seedlings hardening at the same time.
The above description is only exemplary of the present invention and should not be taken as limiting the scope of the present invention, as any modifications, equivalents, improvements and the like made within the spirit and principles of the present invention are intended to be included within the scope of the present invention.
Claims (5)
1. The utility model provides a three-dimensional device of strengthening seedling that takes root of hybrid paper mulberry tissue culture seedling which characterized in that includes:
a water storage tank built in the greenhouse; a Dutch net is laid above the water storage tank; the Dutch net is a support, and an agricultural rock wool layer with spaced holes is laid on the Dutch net; the periphery of the three-layer three-dimensional culture rack is supported by a fixed frame, three layers of three-dimensional culture racks with the interval of 40cm are built, each layer takes a dutch net as a support and rock wool with the interval of 5cm and the thickness of 2cm as a surface, and each layer is provided with a micro-spray head;
sugar-free nutrient solution is filled in the water storage tank; the sugar-free nutrient solution is pumped out by a water pump to the rock wool layer, and the missed sugar-free nutrient solution layer flows into the water storage tank through the saturated rock wool layer.
2. The three-dimensional rooting and seedling strengthening device for tissue culture seedlings of hybrid broussonetia papyrifera as claimed in claim 1, wherein the water pump controls the time and times of the micro-spray heads through an integrated mechanical timer.
3. The device for three-dimensional rooting and seedling strengthening of tissue culture seedlings of hybrid broussonetia papyrifera as claimed in claim 1, wherein the water storage tank is a cement tank with a brick-concrete structure.
4. The three-dimensional rooting and seedling strengthening device for tissue culture seedlings of hybrid paper mulberry as claimed in claim 1, wherein the sugar-free WPM nutrient solution layer is provided to the rock wool layer by a water pump, a PVC pipe, a micro-spray nozzle and spray.
5. The three-dimensional rooting and seedling strengthening device for tissue culture seedlings of hybrid paper mulberry as claimed in claim 1, wherein the bottom of the water storage tank for rooting and seedling raising is a cuboid sealing device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920445617.2U CN209845910U (en) | 2019-04-03 | 2019-04-03 | Three-dimensional rooting and seedling strengthening device for tissue culture seedlings of hybrid paper mulberry |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920445617.2U CN209845910U (en) | 2019-04-03 | 2019-04-03 | Three-dimensional rooting and seedling strengthening device for tissue culture seedlings of hybrid paper mulberry |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209845910U true CN209845910U (en) | 2019-12-27 |
Family
ID=68936066
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920445617.2U Expired - Fee Related CN209845910U (en) | 2019-04-03 | 2019-04-03 | Three-dimensional rooting and seedling strengthening device for tissue culture seedlings of hybrid paper mulberry |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209845910U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110089432A (en) * | 2019-05-15 | 2019-08-06 | 上海离草科技有限公司 | Paper mulberry sugar-free tissue culture method based on environmental factor regulation |
-
2019
- 2019-04-03 CN CN201920445617.2U patent/CN209845910U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110089432A (en) * | 2019-05-15 | 2019-08-06 | 上海离草科技有限公司 | Paper mulberry sugar-free tissue culture method based on environmental factor regulation |
| CN110089432B (en) * | 2019-05-15 | 2022-09-09 | 上海离草科技有限公司 | Sugar-free tissue culture seedling method for paper mulberry based on environmental factor regulation |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102498905B (en) | Method for quick cultivation of stock of long-stem tree-like rose and quick cultivation of long-stem tree-like rose using cutting with long shoots | |
| CN102318547B (en) | Overhead cultivation method of Dendrobium huoshanense test-tube seedlings | |
| CN101395991B (en) | Method for propagating Millettia speciosa Champ. seedlings using cottage | |
| CN103918536A (en) | Planting method for Dendrobium officinale | |
| CN102293111A (en) | Method for raising seedlings by cutting olives | |
| CN103444531B (en) | A kind of quick-breeding method of silkwood tissue cultures | |
| CN103931393B (en) | A kind of method utilizing waste material of edible mushroom to cultivate Taxus x media bag seedling | |
| CN104145707A (en) | Method for cultivating seedlings by means of green branches under full exposure and light mist condition | |
| CN104541906A (en) | Container nursery method for white bark pine seeds | |
| CN106386353A (en) | Seedling growing method of tender branch cutting for keteleeria cyclolepis flous | |
| CN105028089A (en) | Hard-wood cutting seedling method for Lagerstroemia indica Dynamite | |
| CN104956869B (en) | A kind of cotton seedling raising transplantation method | |
| CN102144526A (en) | Detoxified seedling stem matrix floating seedling culturing method for potato | |
| CN102732596A (en) | Cotton verticillium wilt resistant seedling stage identification method | |
| CN104025830A (en) | Greenhouse cherry cultivation method | |
| CN110338049A (en) | The cloud and mist breeding method of cucumber | |
| CN107018896A (en) | A kind of method of facility cuttage tilia miqueliana | |
| CN107409670A (en) | A kind of tobacco floating seed rearing pulls out method for transplanting under plant film | |
| CN103650818A (en) | Standing frame cultivation method of anthurium cut flowers | |
| CN107853046A (en) | A kind of wet-land pine tree industrial cuttage method for culturing seedlings | |
| CN107182754A (en) | A kind of container soilless culture technique of tree peony | |
| CN106942063A (en) | A kind of paulownia tissue culture and rapid propagation method of combination outside sprout-cultivating-bottle technology | |
| CN105493993B (en) | The fast breeding method of Queensland nut stock | |
| CN106332757A (en) | Dendrobe seedling culture technology | |
| CN209845910U (en) | Three-dimensional rooting and seedling strengthening device for tissue culture seedlings of hybrid paper mulberry |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20191227 |