CN207342715U - Radiological predication fallout plot flow control plate and its micro-fluidic radio-immunity tester - Google Patents
Radiological predication fallout plot flow control plate and its micro-fluidic radio-immunity tester Download PDFInfo
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- CN207342715U CN207342715U CN201721091648.XU CN201721091648U CN207342715U CN 207342715 U CN207342715 U CN 207342715U CN 201721091648 U CN201721091648 U CN 201721091648U CN 207342715 U CN207342715 U CN 207342715U
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Abstract
The utility model discloses a kind of radiological predication fallout plot flow control plate and its micro-fluidic radio-immunity tester, micro-fluidic plate includes core plate and bottom plate, Quick Response Code is provided with bottom plate, core plate is equipped with sample area, radioactively labelled substance sample application zone and detection zone, the radioactively labelled substance sample application zone is connected with sample area, detection zone respectively, and detection zone is equipped with detection reaction zone and reaction reference tape.Micro-fluidic radio-immunity tester includes detection case and operation console, the operation console is hinged with detection case, the both sides of detection case are provided with micro-fluidic plate socket and printing single outlet, micro-fluidic plate socket and printing list exit are provided with micro-fluidic plate transport mechanism and printer, and two-dimensional code scanning mechanism and radiological measuring mechanism are provided with above the mobile route of micro-fluidic plate；Controller is provided with the operation console, controller is connected with two-dimensional code scanning mechanism, micro-fluidic plate transport mechanism, radiological measuring mechanism, display screen, communication module and printer.The utility model is conducive to Scanning Detction of the micro-fluidic radio-immunity tester to the radioactive intensity of micro-fluidic plate, and secondary plus radioactively labelled substance method is adapted to micro-fluidic radioimmunoassay method.
Description
Technical field
Medical science is the utility model is related to, more particularly to a kind of radiological predication fallout plot flow control plate and its micro-fluidic radiation
Immunoassay instrument.
Background technology
Radioimmunoassay detection technology is started using an immuno-labelling technique earlier, the spy of radioimmunoassay detection technology
Putting is：High sensitivity, sample that can be very low to antibody content are detected.Compared with other existing immunoassay technologies, than
Such as fluorescence immunoassay detection, the detection sensitivity higher of radioimmunoassay detection, and reagent cost also detect low compared with fluorescence immunoassay.
But there is also some shortcomings for existing radioimmunoassay detection technology：For example have potential radiocontamination possibility,
Since it is desired that with radioactive substance labelled antigen, so, the discarded object after detection has radioactivity.Existing radioimmunoassay inspection
Survey be by sample, radio-labelled antigen and a certain amount of antibody add test tube, after then carrying out a series of troublesome operation
Whole detection process could be completed, the testing time is also longer.After having detected, the discarded object of generation is also more.Because discarded object
With radioactivity, so these discarded objects need special recycling, this just to discarded object recycling bring it is larger
Difficulty.
Utility model content
To solve above technical problem, the utility model provides a kind of radiological predication fallout plot flow control plate and its micro-fluidic radio-immunity
Tester, micro-fluidic radio-immunity tester directly acquire standard curve by the Quick Response Code on radiological predication fallout plot flow control plate, and
The micro-fluidic radio-immunity tester uses radiological predication fallout plot flow control plate, easy to operate, detects sample and label is few, after detection
Scrap concrete is simple.
Technical solution is as follows：
A kind of radiological predication fallout plot flow control plate, is provided with chromatography card shell, which is made of core plate and bottom plate, described
Core plate button is overlayed on bottom plate, and sample application zone, label area and detection zone are equipped with successively along the flow direction of liquid on the core plate,
Its key is：The label area is radioactively labelled substance sample application zone；
Core plate where the sample application zone is equipped with well, and sample application zone passes through mixture runner and the radioactive label
Thing sample application zone connects；
Core plate where the radioactively labelled substance sample application zone is equipped with radioactively labelled substance injection hole, the radioactive label
Thing sample application zone is connected by microchannel with detection zone, and the core plate where detection zone is equipped with test detection window；
On the detection zone detection reaction zone and reaction reference tape are equipped with successively respectively along the flow direction of liquid.
Using the above structure, by gathering the 2 D code information on micro-fluidic plate bottom plate with regard to corresponding detection parameters can be obtained
Data, which is used to calculate and controller operating parameter.
Sample is added into micro-fluidic plate by well, radioactive label is added by radioactively labelled substance injection hole
Thing, after the two hybrid reaction, under the action of capillary effect, radioactive mixture is to the detection reaction zone on detection zone and reaction
Reference tape flows.Testing number evidence can obtain by the radioactive intensity for detecting detection zone, by contrasting detection supplemental characteristic and
Data are detected with regard to that can draw test result.
Further, it is provided with Quick Response Code on the core plate.
Using the above structure, by scanning the two-dimensional code, the detection parameters information of micro-fluidic plate, detection parameters letter can be obtained
Breath includes detecting control parameter and detects data reduced parameter, and detection terminal can be controlled to micro-fluidic plate by detecting control parameter
Accurately detected.Contrasted by detecting reduced parameter with detection data, testing result can be obtained.
Further, the mixture runner is formed by the gap between core plate and bottom plate, the bottom plate and the inspection
Survey forms miniflow siphon passage between area, and the liquid feeding end of the miniflow siphon passage passes through the microchannel and the radioactivity mark
Remember the connection of thing sample application zone.
Using the above structure, under the action of capillary effect, radioactive mixture is automatically along miniflow siphon passage to inspection
Survey area's flowing.
Further, the core plate between the sample application zone and radioactively labelled substance sample application zone is equipped with sample liquid deceleration area,
All mixture runners are set by the sample liquid deceleration area, the sample application zone and the interior circulating direction along liquid of liquid deceleration area
There is sample liquid deceleration boss；
The liquid feeding end of the detection zone is equipped with mixed liquor flow control area, and mixed liquor is equipped with the mixed liquor flow control area
Deceleration boss；
The sample application zone, radioactively labelled substance sample application zone, sample liquid deceleration area, mixed liquor flow control area are recessed upwards respectively
Channel-shaped is formed, the cup depth of the sample application zone is less than the cup depth of the sample liquid deceleration area, the mixed liquor flow control
The cup depth in area is less than the cup depth of the radioactively labelled substance sample application zone, the core plate of the sample liquid deceleration area both sides
On be respectively equipped with air hole.
Using the above structure, the liquid flow velocity in miniflow siphon passage can be controlled by flow control passage, is avoided
React too fast.Mixed liquor deceleration boss and sample liquid deceleration boss slow down the speed of test liquid, and sample liquid and label can be given abundant
Reaction time.
Air pressure balance in the passages such as split channel, miniflow siphon passage is maintained by air hole, it is ensured that liquid communication is suitable
Freely.
Further, the core plate is equipped with waste collection area, outlet end and the waste liquid of the miniflow siphon passage
Collecting region connects, and waste liquid control boss is equipped with the waste collection area, the waste liquid control boss of waste collection area inlet
Diameter is more than the diameter of the waste liquid control boss in waste collection area exit.
Using the above structure, because radioactive element is hazardous to the human body, the waste liquid after convenient detection, which is concentrated, to be received
Collection gets up conveniently to be uniformly processed, while the control boss set in waste collection area plays the flowing velocity and sample liquid of control sample liquid
The effect of flow, to ensure the enough reaction time needed for immune response, guarantee reaches the complete and abundant of immune response.
A kind of micro-fluidic radio-immunity tester, is provided with detection case, its key is：The front end face of the detection case is opened
There is micro-fluidic plate socket, for inserting radiological predication fallout plot flow control plate, which is provided with transport mechanism, micro-fluidic
Two-dimensional code scanning mechanism and radiological measuring mechanism are provided with above the mobile route of plate；
The radiological measuring mechanism is provided with anti-interference lead, is provided with ray entrance aperture on the anti-interference lead, penetrates
Radiation detection devices are provided with line entrance aperture；
The detection case top surface is embedded with control panel, the control panel, transport mechanism, two-dimensional code scanning mechanism, radiation
Property detection device is connected with controller respectively.
Using the above structure, the 2 D code information on micro-fluidic plate can be scanned by two-dimensional code scanning mechanism.Controller root
Control micro-fluidic plate transport mechanism that micro-fluidic plate is transmitted to test position according to detection control parameter, so easy to radiological measuring
Mechanism is detected micro-fluidic plate.
Controller need not store substantial amounts of interpretation standard curve according to detection reduced parameter, controller, it is not required that logical
Artificial selection interpretation standard curve is crossed, intelligence degree is high.
Further, the anti-interference lead is vertically fixed on above the transport mechanism by supporting rack, described
Ray entrance aperture is arranged on the central position of the anti-interference lead bottom surface and vertically downward；
The radiation detection devices are provided with scintillation crystal, photo-coupler and photomultiplier, the scintillation crystal, optocoupler
Clutch and photomultiplier are set gradually from top to bottom, and the light that the scintillation crystal is sent synthesizes Ray Of Light through photo-coupler
After propagate to photomultiplier, the output terminal of the photomultiplier is connected with the controller.
Using the above structure, because ray entrance aperture very little and perpendicular to lead set, radiological measuring mechanism
Face and the radioactivity in the region of ray entrance aperture can only be detected, avoids the radioactive ray Interference Detection that other regions are sent.
Further, printer is additionally provided with the detection case, the controller drives by printing drive circuit
Printer prints, and the controller is communicated by radio communication device with cloud data center.
Using the above structure, testing result can be printed, is led to by the way that radio communication device is convenient with cloud data center
Letter, detection terminal will detect data, testing result and 2 D code information and be sent to cloud data center, and cloud data center ties detection
Fruit is confirmed.
Beneficial effect：Radiological predication fallout plot flow control plate and its micro-fluidic radio-immunity tester using the utility model, due to
Radioactive element is short-half-life, is not suitable for wrapping standby method in advance using common fluorescent labeled antibody or antigen, due to micro-
Sample well and labelled antibody hole are reserved on flow control plate, it is no longer necessary to coating is prepared in advance, directly by radioactive label during detection
Antibody, sample are added dropwise in label pond and sample cell respectively, and sample is entered firm with the inside in label pond by microchannel
The radiolabelled antibody of addition directly reacts, and radiolabelled antibody is no longer restricted in the use of field of fast detection.
Brief description of the drawings
Fig. 1 is the top view of the micro-fluidic plate of the utility model；
Fig. 2 is the structure diagram of the core plate of the utility model；
Fig. 3 is the profile of the micro-fluidic plate of the utility model；
Fig. 4 is the partial enlarged view at a in Fig. 3；
Fig. 5 is the overall three-dimensional structure diagram of micro-fluidic radio-immunity tester；
Fig. 6 is the internal structure schematic diagram of micro-fluidic radio-immunity tester；
Fig. 7 is the structural scheme of mechanism of radiological measuring mechanism；
Fig. 8 is the circuit diagram of transmitting circuit.
Embodiment
With reference to embodiment and attached drawing, the utility model is described in further detail.
As shown in Figs 1-4, a kind of radiological predication fallout plot flow control plate, including transparent core plate 1, the core plate 1 button cover on a base plate 2,
Quick Response Code 3 is provided with 1 top surface of core plate, which is equipped with sample area 4, radiation along the flow direction of liquid successively
Property label sample application zone 5, detection zone 6 and waste collection area 18, well 7, radioactively labelled substance sample-adding are provided with the sample area 4
Radioactively labelled substance injection hole 8 is provided with area 5；
The sample area 4 is connected by mixture runner 9 with radioactively labelled substance sample application zone 5, which adds
Sample area 5 is connected by flow control passage 10 with detection zone 6, and inspection is equipped with successively along the flow direction of liquid on the detection zone 6
Survey reaction zone 11 and reaction reference tape 12.
The mixture runner 9 is equipped with sample liquid deceleration area 14, and the depth of the sample liquid deceleration area 14 is more than the sample area 4
Depth, the circulating direction in the sample area 4 and sample liquid deceleration area 14 along liquid is equipped with sample liquid deceleration boss 15；
Miniflow siphon passage 13 is formed between the bottom plate 2 and detection zone 6, the liquid feeding end of the miniflow siphon passage 13 leads to
Cross flow velocity control passage 10 to connect with the radioactively labelled substance sample application zone 5, which is equipped with mixing liquid stream
Fast control zone 16, the depth in the mixed liquor flow control area 16 is less than the depth of the radioactively labelled substance sample application zone 5, described mixed
Close in flow velocity control zone 16 and be equipped with mixed liquor deceleration boss 17.
The outlet end of the miniflow siphon passage 13 is connected with the waste collection area 18, is equipped with the waste collection area 18
Waste liquid controls boss 19, and the diameter of the waste liquid control boss 19 of 18 inlet of waste collection area goes out more than the waste collection area 18
The diameter of waste liquid control boss 19 at mouthful.Air hole 20 is additionally provided with the core plate 1, which is located at the sample liquid and subtracts
The both sides in fast area 14.
As viewed in figures 5-8, a kind of micro-fluidic radio-immunity tester, including detection case 21, the front end face of the detection case 21
Micro-fluidic plate socket 22 is provided with, transport mechanism 23 is provided with the micro-fluidic plate socket 22, which is used to transmit micro-
Flow control plate, is provided with two-dimensional code scanning mechanism 24 and radiological measuring mechanism 25 above the mobile route of micro-fluidic plate, this two
Dimension code sweep mechanism 24 is used to scan the Quick Response Code 3 on the micro-fluidic plate, which is used to detect miniflow
Control the detection reaction zone 11 of plate and react the radioactivity of reference tape 12；
21 top surface of detection case is embedded with control panel 26, and the control panel 26, transport mechanism 23, Quick Response Code 3 scan
Mechanism 24, radiological measuring mechanism 25 are connected with controller 27 respectively, which is arranged in the detection case 21.
Printer 36 is additionally provided with the detection case 21, the controller 27 is by printing drive circuit driving printing
Machine 36 prints, and the controller 27 is communicated by radio communication device with cloud data center.
The radiological measuring mechanism 25 includes anti-interference lead 30, the anti-interference lead 30 it is vertical by supporting rack 31
The top of transport mechanism 23 is fixed on, the central position that the anti-interference lead 30 is right against transport mechanism 23 is provided with ray
Entrance aperture 32, is disposed with scintillation crystal 33, photo-coupler 34 and photomultiplier in the ray entrance aperture 32 from top to bottom
35, the light which sends is sent to photomultiplier 35, the light after photo-coupler 34 synthesizes Ray Of Light
The output terminal of electric multiplier tube 35 is connected with the controller 27.
The transport mechanism 23 includes stepper motor 28 and micro-fluidic board binding 29, which drives micro-fluidic
Board binding 29 drives the micro-fluidic plate to move back and forth, and the controller 27 controls the stepper motor 28 by transmitting circuit
Work.
The transmitting circuit includes operational amplifier U1 and operational amplifier U2, the in-phase input end of operational amplifier U1 with
The path clustering end group connection of operation console, inverting input are connected through resistance R1 with power supply, and inverting input also connects through resistance R2
Ground, the output terminal of operational amplifier U1 are connected through base stages of the resistance R3 respectively with triode D1 and triode D2, triode D1's
Collector is connected with pulse power supply, and emitter connects the cathode of the stepper motor 28, the anode of stepper motor 28 and three poles
The collector connection of pipe D2, the emitter ground connection of triode D2；
The inverting input of operational amplifier U2 is connected with controller 27, and in-phase input end is connected through resistance R4 with power supply,
In-phase input end is also grounded through resistance R5, the output terminal of operational amplifier U2 through resistance R6 respectively with triode D3 and triode D4
Base stage connection, the collector of triode D3 be connected with pulse power supply, and the anode of the emitter connection stepper motor 28, walks
The cathode of stepper motor 28 is connected with the collector of triode D4, the emitter ground connection of triode D4.
Finally it should be noted that foregoing description is only the preferred embodiment of the utility model, the common skill of this area
Art personnel on the premise of without prejudice to the utility model aims and claim, can make under the enlightenment of the utility model
Expression, such conversion are each fallen within the scope of protection of the utility model as multiple types.
Claims (9)
1. a kind of radiological predication fallout plot flow control plate, sets core plate (1) and bottom plate (2), the core plate button to overlay on bottom plate (2), the core plate
(1) sample application zone (4), label area and detection zone (6) are equipped with successively along the flow direction of liquid on, it is characterised in that：It is described
Label area is radioactively labelled substance sample application zone (5)；
Core plate (1) where the sample application zone (4) is equipped with well (7), and sample application zone (4) pass through mixture runner (9) and institute
State radioactively labelled substance sample application zone (5) connection；
Core plate (1) where the radioactively labelled substance sample application zone (5) is equipped with radioactively labelled substance injection hole (8), the radiation
Property label sample application zone (5) connected by microchannel (10) with detection zone (6), the core plate (1) where detection zone (6) be equipped with survey
Examination detection window；
On the detection zone (6) detection reaction zone (11) and reaction reference tape are equipped with successively respectively along the flow direction of liquid
(12)。
2. radiological predication fallout plot flow control plate according to claim 1, it is characterised in that：Quick Response Code is provided with the core plate (1)
(3)。
3. radiological predication fallout plot flow control plate according to claim 1, it is characterised in that：The mixture runner (9) is by core plate (1)
Gap between bottom plate (2) is formed, and miniflow siphon passage (13), institute are formed between the bottom plate (2) and the detection zone (6)
The liquid feeding end for stating miniflow siphon passage (13) is connected by the microchannel (10) with the radioactively labelled substance sample application zone (5).
4. radiological predication fallout plot flow control plate according to claim 1, it is characterised in that：The sample application zone (4) and radioactive label
Core plate (1) between thing sample application zone (5) is equipped with sample liquid deceleration area (14), and all mixture runners (9) pass through the sample liquid
Circulating direction in deceleration area (14), the sample application zone (4) and liquid deceleration area (14) along liquid is equipped with sample liquid deceleration boss
(15)；
The liquid feeding end of the detection zone (6) is equipped with mixed liquor flow control area (17), is set in the mixed liquor flow control area (17)
There is mixed liquor deceleration boss (16)；
The sample application zone (4), radioactively labelled substance sample application zone (5), sample liquid deceleration area (14), mixed liquor flow control area (17) point
Depression does not form channel-shaped not upwards, and the cup depth of the sample application zone (4) is less than the cup depth of the sample liquid deceleration area (14), institute
The cup depth for stating mixed liquor flow control area (17) is less than the cup depth of the radioactively labelled substance sample application zone (5), described
Air hole (20) is respectively equipped with the core plate (1) of sample liquid deceleration area (14) both sides.
5. radiological predication fallout plot flow control plate according to claim 3, it is characterised in that：The core plate (1) is equipped with waste collection
Pond (18), the outlet end of the miniflow siphon passage (13) are connected with the waste collection pond (18), the waste collection pond (18)
It is interior to be equipped with waste liquid control boss (19)；
The diameter of the waste liquid control boss (19) of waste collection pond (18) inlet goes out more than the waste collection pond (18)
The diameter of waste liquid control boss (19) at mouthful.
6. a kind of micro-fluidic radio-immunity tester, is provided with detection case (21), it is characterised in that：The front end of the detection case (21)
Face is provided with micro-fluidic plate socket (22), and for inserting micro-fluidic plate, which is provided with transport mechanism
(23), two-dimensional code scanning mechanism (24) and radiological measuring mechanism (25) are provided with above the mobile route of micro-fluidic plate；
The radiological measuring mechanism (25) is provided with anti-interference lead (30), and being provided with ray on the anti-interference lead (30) enters
Perforation (32), ray entrance aperture (32) is interior to be provided with radiation detection devices；
Detection case (21) top surface is embedded with control panel (26), and the control panel (26), transport mechanism (23), Quick Response Code are swept
Mechanism (24), radiation detection devices are retouched to be connected with controller (27) respectively.
7. micro-fluidic radio-immunity tester according to claim 6, it is characterised in that：The anti-interference lead (30) is led to
Cross supporting rack (31) to be vertically fixed on above the transport mechanism (23), the ray entrance aperture (32) is arranged on described anti-interference
The central position of lead (30) bottom surface and vertically downward；
The radiation detection devices are provided with scintillation crystal (33), photo-coupler (34) and photomultiplier (35), the flicker
Crystal (33), photo-coupler (34) and photomultiplier (35) are set gradually from top to bottom, what the scintillation crystal (33) sent
Light propagates to photomultiplier (35), the output of the photomultiplier (35) after photo-coupler (34) synthesizes Ray Of Light
End is connected with the controller (27).
8. micro-fluidic radio-immunity tester according to claim 6, it is characterised in that：The transport mechanism (23) is provided with
Stepper motor (28) and micro-fluidic board binding (29), the stepper motor (28) are driven described in micro-fluidic board binding (29) drive
Micro-fluidic plate moves back and forth, and the controller (27) controls the stepper motor (28) to work by transmitting circuit.
9. micro-fluidic radio-immunity tester according to claim 6, it is characterised in that：Also set up in the detection case (21)
There is printer (36), the controller (27) drives printer (36) to print by printing drive circuit, the controller (27)
Communicated by radio communication device with cloud data center.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721091648.XU CN207342715U (en) | 2017-08-29 | 2017-08-29 | Radiological predication fallout plot flow control plate and its micro-fluidic radio-immunity tester |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721091648.XU CN207342715U (en) | 2017-08-29 | 2017-08-29 | Radiological predication fallout plot flow control plate and its micro-fluidic radio-immunity tester |
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| Publication Number | Publication Date |
|---|---|
| CN207342715U true CN207342715U (en) | 2018-05-11 |
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ID=62412156
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721091648.XU Active CN207342715U (en) | 2017-08-29 | 2017-08-29 | Radiological predication fallout plot flow control plate and its micro-fluidic radio-immunity tester |
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| CN (1) | CN207342715U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110302847A (en) * | 2019-06-12 | 2019-10-08 | 苏州迪芬德物联网科技有限公司 | A kind of Internet of Things Teaching Experiment Box |
| CN111505318A (en) * | 2020-05-15 | 2020-08-07 | 刘大基 | Blood transfusion compatibility detection package analyzer |
-
2017
- 2017-08-29 CN CN201721091648.XU patent/CN207342715U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110302847A (en) * | 2019-06-12 | 2019-10-08 | 苏州迪芬德物联网科技有限公司 | A kind of Internet of Things Teaching Experiment Box |
| CN111505318A (en) * | 2020-05-15 | 2020-08-07 | 刘大基 | Blood transfusion compatibility detection package analyzer |
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