CN210109119U - Immunofluorescence quantitative analyzer - Google Patents
Immunofluorescence quantitative analyzer Download PDFInfo
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- CN210109119U CN210109119U CN201920922146.XU CN201920922146U CN210109119U CN 210109119 U CN210109119 U CN 210109119U CN 201920922146 U CN201920922146 U CN 201920922146U CN 210109119 U CN210109119 U CN 210109119U
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Abstract
The utility model discloses an immunofluorescence quantitative analyzer, which comprises a shell, wherein a touch panel is arranged on the upper surface of the shell, a card placing hole is arranged on the lower part of the shell, and a recovery box is arranged on the side wall of the shell; a sample injection mechanism and a light source module are arranged in the shell; the photoelectric conversion module, the control analysis module, the display module and the touch panel are sequentially in communication connection, and the network connection module and the data storage module are respectively in communication connection with the control analysis module; the sample introduction mechanism comprises two rolling shafts, a transmission belt is sleeved outside the two rolling shafts, each rolling shaft is connected with a first motor for driving the rolling shaft to rotate, the first motor is in communication connection with the control analysis module, a guide plate is arranged below the right end of the transmission belt, the guide plate is obliquely arranged, the lower end of the guide plate extends into the recovery box, the detection efficiency is stable due to the mode of automatically conveying the reagent card, and the reagent card is automatically recovered.
Description
Technical Field
The utility model belongs to the technical field of medical instrument detects, concretely relates to immunofluorescence quantitative analysis appearance.
Background
The immunofluorescence detection technology is a rapid detection technology commonly used in the biomedical detection at present, and has the main advantages of strong specificity, high sensitivity, high speed and the like. It is an immunolabeling technology that was first established by Coons in the early 40 s of the 20 th century. The basic principle is that fluorescent substance is used as a marker, and the sensitivity and the detectability of fluorescence are combined with the high specificity reaction of antigen and antibody. Fluorescent substances are capable of absorbing light energy into an excited state under the action of excitation light of a specific wavelength, releasing the previously absorbed light energy in the form of electromagnetic radiation, and generating fluorescence. The specific fluorescence can be directly observed by a fluorescence microscope, can also be received by a photoelectric converter and converted into an electric signal to be further processed, can accurately, sensitively and quickly position and detect certain trace or ultra-trace substances, and is widely applied to a plurality of fields of medicine, biology, environmental protection and the like.
Several specific fluorescent immunoassays have been developed in recent years and have found application in clinical testing as well as enzyme immunoassays and radioimmunoassays.
The quantum dot has special optical characteristics compared with the traditional organic conventional fluorescent agent, ① quantum dot has the characteristic that the fluorescence emission wavelength can be adjusted by changing the size and the composition of the quantum dot, the excitation spectrum is wide and continuous, the absorption coefficient is large, the fluorescence intensity is high, the fluorescence emission peak is narrow and symmetrical, and the long-wave tailing is avoided. ② quantum dot has larger Stokes displacement.
The sample introduction module of the existing immunofluorescence quantitative analyzer adopts a semi-automatic sample introduction timing reminding device which can automatically set time length, automatically remind the next detection after the last detection result is finished and can generate a booming sound, and the semi-automatic sample introduction device is characterized in that a plurality of detection cards can be placed on a platform in parallel, one of the detection cards is placed in a sample introduction groove, the other detection cards are parallel to the sample introduction groove, when the detection cards in the sample introduction groove are tested and pulled out, a spring clamping plate of the semi-automatic sample introduction device can push the test cards to enter the sample introduction groove to complete the test until all the test cards are completely tested, and the semi-automatic sample introduction device can introduce 1-20 samples at one time.
The aforesaid advance a kind module need the manual work to be extracted the detection card and just can make earlier a test card get into the introduction groove and detect, this just needs special one to extract the detection card alone, leads to operating personnel must all be on this immunofluorescence quantitative analysis appearance next door at any time, and operating personnel's operation is fast slow and concentration degree all can influence detection efficiency.
SUMMERY OF THE UTILITY MODEL
In order to solve the problem that the operation speed of the sample introduction module of the prior art needs manual operation and the operation personnel who exists is fast and slow and concentration degree can all influence detection efficiency, the utility model provides a can automatic propelling movement reagent card and can collect the immunofluorescence quantitative analysis appearance of reagent card.
The utility model discloses the technical scheme who adopts does: an immunofluorescence quantitative analyzer comprises a shell, wherein a touch panel is arranged on the upper surface of the shell, a card placing hole is formed in the lower portion of the shell, and a recovery box is arranged on the side wall of the shell;
a sample introduction mechanism, a light source module, a photoelectric conversion module, a control analysis module, a display module, a network connection module and a data storage module are arranged in the shell; the photoelectric conversion module, the control analysis module, the display module and the touch panel are sequentially in communication connection, and the network connection module and the data storage module are respectively in communication connection with the control analysis module;
the sampling mechanism comprises two rolling shafts, transmission belts are sleeved outside the two rolling shafts, each rolling shaft is connected with a first motor for driving the rolling shaft to rotate, the first motor is in communication connection with the control analysis module, a guide plate is arranged below the right end of each transmission belt, the guide plate is obliquely arranged, and the lower end of the guide plate extends into the recovery box.
Further limited, the immunofluorescence quantitative analyzer further comprises an SD chip storage interface and a two-dimensional code generation module which are in communication connection with the data storage module, and the two-dimensional code generation module is in communication connection with the data storage module.
Further, the light source module is a laser light source.
Further define, the photoelectric conversion module is including the holophote, convex lens, optical filter, grating and the photoelectric converter that set gradually, photoelectric converter with control analysis module communication is connected.
Further limiting, the control analysis module comprises a data analyzer and an electrical control main board, and the data analyzer is respectively in communication connection with the display module and the data storage module; the electric control main board is in communication connection with the first motor.
Further define, the network connection module includes wifi transmitter and computer connection port, computer connection port set up in on the casing.
The utility model has the advantages that: the automatic detection area of sending into of reagent card can be accomplished in the cooperation through control analysis module, transmission band, roller bearing and first motor, only need from putting the card hole put into the reagent card on the transmission band can, specifically be: when the detection information of the previous reagent card is transmitted to the control analysis module, the control analysis module analyzes the transmitted detection information, after the analysis is finished, the control analysis module sends starting information to the first motors, the two first motors are started to drive the rolling shafts to rotate, the rolling shafts drive the rolling shafts to move, when the reagent card to be detected on the transmission belt moves to a detection area, the detected information is transmitted to the control analysis module through the photoelectric conversion module, the control analysis module sends an instruction to stop the two first motors, the two first motors stop rotating, the rolling shafts stop rotating, the transmission belt stops rotating, and the detected reagent card enters the recovery box through the guide plate to be automatically recovered; repeating the above steps after the detection of the reagent card is completed; the mode of automatically conveying the reagent card ensures that the detection efficiency is stable and the automatic recovery of the reagent card is realized.
Drawings
Fig. 1 is a schematic structural diagram of the present invention;
FIG. 2 is a cross-sectional view B-B of FIG. 1;
FIG. 3 is an enlarged view of area A of FIG. 2;
in the figure: 1-a shell; 2-a touch panel; 3-placing a clamping hole; 4-recovery box; 5-a power supply connector; 6, conveying the belt; 7-a roller; 8-reagent card; 9-a guide plate; 10-a laser light source; 11-a total reflection mirror; 12-convex lens; 13-a filter; 14-a grating; 15-a photoelectric converter; 16-a control analysis module; 17-a network connection module; 18-a display module; 19-first motor.
Detailed Description
The present invention will be further described with reference to the accompanying drawings and specific embodiments.
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary and intended to be used for explaining the present invention, and should not be construed as limiting the present invention.
In the description of the present invention, it is to be understood that the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implying any number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.
In the present invention, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
In the description of the present invention, some structures or devices are not specifically described, and it is understood that there are structures or devices that can be implemented in the prior art.
Example 1
The technical scheme of the embodiment is as follows: an immunofluorescence quantitative analyzer comprises a shell 1, wherein a touch panel 2 is arranged on the upper surface of the shell 1, a card placing hole 3 is formed in the lower part of the shell 1, and a recovery box 4 is arranged on the side wall of the shell 1; a sample introduction mechanism, a light source module, a photoelectric conversion module, a control analysis module 16, a display module 18, a network connection module 17 and a data storage module are arranged in the shell 1; the photoelectric conversion module, the control analysis module, the display module and the touch panel 2 are sequentially in communication connection, and the network connection module and the data storage module are respectively in communication connection with the control analysis module; the sampling mechanism comprises two rolling shafts 7, a transmission belt 6 is sleeved outside the two rolling shafts 7, each rolling shaft 7 is connected with a first motor 19 for driving the rolling shaft 7 to rotate, the first motor 19 is in communication connection with the control analysis module, a guide plate 9 is arranged below the right end of the transmission belt 6, the guide plate 9 is obliquely arranged, and the lower end of the guide plate extends into the recovery box 4. The immunofluorescence quantitative analyzer further comprises an SD chip storage interface and a two-dimensional code generation module which are in communication connection with the data storage module, and the two-dimensional code generation module is in communication connection with the data storage module. The light source module is a laser light source 10. The photoelectric conversion module comprises a total reflector 11, a convex lens 12, a filter 13, a grating 14 and a photoelectric converter 15 which are sequentially arranged, and the photoelectric converter 15 is in communication connection with the control analysis module. The control analysis module 16 comprises a data analyzer and an electric control main board, and the data analyzer is respectively in communication connection with the display module and the data storage module; the electric control main board is respectively in communication connection with the first motor 19 and the feeding mechanism. The network connection module 17 comprises a wifi emitter and a computer connection port, and the computer connection port is arranged on the shell 1.
As shown in fig. 1-3, a reagent card 8 is placed on a conveyor belt 6 through a card placing hole 3, a plurality of reagent cards 8 can be placed on the conveyor belt 6 at the same time, in this embodiment, two reagent cards 8 are placed, a laser light source 10 is started, the laser light source 10 emits laser light, the laser light is firstly reflected by a total reflection mirror 11, reflected light enters a detection area on the conveyor belt 6, when no data is displayed on a display module 18, it indicates that a control analysis module 16 does not receive information, at this time, an electrical control main board of the control analysis module 16 sends an instruction, the instruction is transmitted to a first motor 19, the first motor 19 is started, the first motor 19 drives a roller 7 to rotate, the roller 7 drives the conveyor belt 6 to move, so that the reagent cards 8 move to the detection area, when the totally reflected laser light sequentially passes through a convex lens 12, a filter 13 and a grating 14, and finally enters a photoelectric converter 15, the photoelectric converter, data analyzer divides the two tunnel transmission simultaneously with data analysis result and gives network connection module 17 and display module 18, and display module 18 is the display screen, and the top of display screen is provided with touch panel 2, through the instruction in the input touch panel 2 or print, when the selection prints, prints the information transfer and gives the wifi transmitter, and the wifi transmitter gives the printer with information transfer, and the printer accepts information and prints. The tactile feedback system of the touch panel 2 transmits the received signal to the network connection module 17 and the display screen; when data are input into the data storage module, the two-dimensional code generation module can automatically generate a two-dimensional code, the two-dimensional code and the data input in the time period are integrated and displayed in the display screen, and a patient can acquire report information by scanning the two-dimensional code, so that the report information can be conveniently and directly viewed on a mobile phone, and paper is saved; the electrical control main board controls the start and stop of the first motor 19 through a PLC control system.
The software system of the immunofluorescence quantitative analyzer consists of a detection control algorithm program in a machine and analysis and database software based on a PC platform. (the instrument can be connected with PC to export the detected data to PC for storage management, or can be directly stored in memory card without being connected with PC, or can be printed for storage, whether connected or not, and the mode can be selected)
The software system comprises a startup calibration module: automatically checking whether the working state and the static parameters of the instrument are normal or not;
an initialization module: setting the working state initialization of each unit of the system;
the signal acquisition and analysis module: acquiring information of a reagent card signal area, and analyzing and inputting a reagent card correction parameter and a test sample parameter;
a sample testing and analyzing module: collecting information of a test signal area, and calculating the concentration of a detected object by using a pre-programmed algorithm according to the parameters of signal collection and analysis;
a system parameter setting module: setting and changing the working state and parameters of the instrument, detecting modes and the like.
A signal compensation circuit is provided in the photoelectric converter 15 for reducing the interference signal.
The immunofluorescence quantitative analysis system adopts laser with 365nm wavelength, and when the reagent card 8 is irradiated, the fluorescent substance quantum dots can emit light waves of 610 nm.
The immunofluorescence detection technology takes fluorescence quantum dots as markers, and the chromatographic strip is prepared by compounding various materials.
The relation between the optical density value and the concentration is represented by a standard curve, and the control analysis module and the software system can automatically convert the optical density value into a concentration value during each measurement, so that the concentration of an analyte in an unknown sample is analyzed.
The operation mode of the fluorescence quantitative detector comprises the following steps:
firstly, selecting a detection mode required by a detection item after starting up;
secondly, putting the pre-reacted reagent card on a conveying belt 6 through a card placing hole 3 and conveying the reagent card to a detection area;
and thirdly, irradiating the excitation light source emitted from the excitation light source module on a signal area of the to-be-tested agent card, and emitting a fluorescence signal by the fluorescent quantum dots on the signal area under the action of the excitation light.
And fourthly, capturing the fluorescence signal emitted by the signal area by a photoelectric conversion module arranged in the immunofluorescence quantitative analyzer, and completing the conversion of the photoelectric signal by the photoelectric conversion module. The photoelectric conversion module converts light emitted by a test agent card signal area into an electric signal, amplifies the electric signal by an amplifying circuit, and displays a result after the electric signal is processed by an analysis system.
The present invention is not limited to the above-mentioned optional embodiments, and any other products in various forms can be obtained by anyone under the teaching of the present invention, and any changes in the shape or structure thereof, all the technical solutions falling within the scope of the present invention, are within the protection scope of the present invention.
Claims (6)
1. An immunofluorescence quantitative analyzer is characterized by comprising a shell (1), wherein a touch panel (2) is arranged on the upper surface of the shell (1), a card placing hole (3) is formed in the lower portion of the shell (1), and a recovery box (4) is arranged on the side wall of the shell (1);
a sample feeding mechanism, a light source module, a photoelectric conversion module, a control analysis module (16), a display module (18), a network connection module (17) and a data storage module are arranged in the shell (1); the photoelectric conversion module, the control analysis module, the display module and the touch panel (2) are sequentially in communication connection, and the network connection module and the data storage module are respectively in communication connection with the control analysis module;
the sampling mechanism comprises two rolling shafts (7), a transmission belt (6) is sleeved outside the two rolling shafts (7), each rolling shaft (7) is connected with a first motor (19) for driving the rolling shaft to rotate, the first motor (19) is in communication connection with the control analysis module, a guide plate (9) is arranged below the right end of the transmission belt (6), the guide plate (9) is obliquely arranged, and the lower end of the guide plate extends into the recovery box (4).
2. The immunofluorescence quantitative analyzer according to claim 1, further comprising an SD chip storage interface and a two-dimensional code generation module communicatively connected to the data storage module, the two-dimensional code generation module being communicatively connected to the data storage module.
3. The immunofluorescence quantitative analyzer according to claim 1, wherein the light source module is a laser light source (10).
4. The immunofluorescence quantitative analyzer according to claim 1, wherein the photoelectric conversion module comprises a total reflection mirror (11), a convex lens (12), a filter (13), a grating (14) and a photoelectric converter (15) which are arranged in sequence, and the photoelectric converter (15) is in communication connection with the control analysis module.
5. The immunofluorescence quantitative analyzer according to any one of claims 1 to 3, wherein the control analysis module (16) comprises a data analyzer and an electric control main board, and the data analyzer is respectively connected with the display module and the data storage module in a communication way; the electric control main board is in communication connection with the first motor (19).
6. The immunofluorescence quantitative analyzer according to claim 5, wherein the network connection module (17) comprises a wifi emitter and a computer connection port, the computer connection port is disposed on the housing (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920922146.XU CN210109119U (en) | 2019-06-18 | 2019-06-18 | Immunofluorescence quantitative analyzer |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920922146.XU CN210109119U (en) | 2019-06-18 | 2019-06-18 | Immunofluorescence quantitative analyzer |
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| CN210109119U true CN210109119U (en) | 2020-02-21 |
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| CN201920922146.XU Active CN210109119U (en) | 2019-06-18 | 2019-06-18 | Immunofluorescence quantitative analyzer |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115524506A (en) * | 2022-05-25 | 2022-12-27 | 刘兆华 | Virus antigen detection device and using method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115524506A (en) * | 2022-05-25 | 2022-12-27 | 刘兆华 | Virus antigen detection device and using method thereof |
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