CN107462735A - Sample-adding detection method twice based on microflow control technique - Google Patents
Sample-adding detection method twice based on microflow control technique Download PDFInfo
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- CN107462735A CN107462735A CN201710681307.6A CN201710681307A CN107462735A CN 107462735 A CN107462735 A CN 107462735A CN 201710681307 A CN201710681307 A CN 201710681307A CN 107462735 A CN107462735 A CN 107462735A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
- G01N35/00069—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides whereby the sample substrate is of the bio-disk type, i.e. having the format of an optical disk
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
- G01N2035/00099—Characterised by type of test elements
- G01N2035/00158—Elements containing microarrays, i.e. "biochip"
Abstract
The present invention discloses a kind of sample-adding detection method twice based on microflow control technique, including micro-fluidic plate and record interpretoscope, mainly comprise the following steps and labelled antibody or antigen are added dropwise in the label area of micro-fluidic plate first, sample liquid is then dropped in the sample area of micro-fluidic plate, utilized after the X minutes of interval and record the result that interpretoscope reads and analyzes micro-fluidic plate detection zone, the bottleneck problem in label technique for fixing has been got around using the present invention, labelled antibody or antigen are individually packaging, additionally, due to without preparing fixation in advance, this method can be also used for some and be not suitable for carrying out the test event for pre-fixing labelled antibody or antigen in advance.
Description
Technical field
The present invention relates to rapid detection technical field, and in particular to a kind of sample-adding detection side twice based on microflow control technique
Method.
Background technology
With the breakthrough acquired by material science, micro-nano process technology and microelectronics, micro-fluidic chip
Developed rapidly.Micro-fluidic chip (microfluidic chip) is also known as " laboratory on chip " (lab-on-a-
Chip), it is based on micro electro mechanical processing technology, is made on the materials such as silicon chip, glass or dimethyl silicone polymer (PDMS)
Make microchannel network so that controlled fluid flows in microchannel network, so as to realize the reaction in biological and chemical field, divide
From, detection etc. operation.In traditional medicine field of fast detection, label is usually previously fixed to the label of micro-fluidic plate
Area, sample is directly added dropwise in sample area using one-step method detection during detection, utilizes the microflow control technique control reaction time, react
The intensity of fluorescent material is detected after end, and this method needs labelled antibody to be fixed to using special technique due to labelled antibody
In label area, this technical requirements are very high, to reach both be not bonded on bottom plate again rapid uniform dissolution in sample liquid
Generation immune response, while loosely and can not be come off in transportation, once technical elements go wrong, so that it may cause to resist
Body, antigen-reactive are insufficient, influence the accuracy of detection, and time longer rear antibody easily aoxidizes, and causes detection inaccurate even
It can not detect.
The content of the invention
To solve above technical problem, the present invention provides a kind of sample-adding detection method twice based on microflow control technique.
Technical scheme is as follows：A kind of sample-adding detection method twice based on microflow control technique, its key are：Including miniflow
Plate and record interpretoscope are controlled, is detected according to the following steps：Labelled antibody or antigen are added dropwise in the micro-fluidic plate first
Label area, then drops in sample liquid the sample area of micro-fluidic plate, is spaced after X minutes and reads and analyze micro- using recording interpretoscope
The testing result of detection zone on stream control plate.
Using above-mentioned technical proposal, labelled antibody or antigen and to be measured are directly separately added into by two-step method on micro-fluidic plate
Sample liquid, no longer need on micro-fluidic plate to prepare coating in advance, got around the bottleneck problem in label technique for fixing, mark is anti-
Body or antigen are individually packaging, and additionally, due to without preparing fixation in advance, this method can be also used for some and be not suitable for into behaviour
First pre-fix the test event of labelled antibody or antigen.
As preferred：Above-mentioned micro-fluidic plate includes core plate and bottom plate, and the core plate is covered on the bottom plate, the core plate
Medial surface on along the flow direction of sample liquid be provided with the sample area, label area and the detection zone that are sequentially communicated, on the core plate
Counter sample area and label area are respectively equipped with well and label adding hole, along the flowing side of sample liquid on the detection zone
Reaction zone and reaction reference tape are detected to being sequentially provided with.Using program sample liquid by being reserved with well and label on core plate
Adding hole, it is convenient that sample liquid or labelled antibody or antigen is added dropwise.
Above-mentioned reading simultaneously analyzes the method for the result of detection zone on micro-fluidic plate and is：The labelled antibody or antigen are fluorescence
Plain labelled antibody or antigen, detection reaction zone is read respectively and react the fluorescence intensity of reference tape, and root using interpretoscope is recorded
The concentration of target substance in sample liquid is calculated according to standard curve.The specific concentration of target antigen can be directly calculated using the program,
So as to reach the purpose of quantitative detection.
The preparation method of above-mentioned standard curve is：Standard antigen of the configuration with concentration gradient, on the micro-fluidic plate
Label adding hole in fluorescein labelled antibody is added dropwise after, the standard antigens of various concentrations, interval X point are added dropwise in well
Utilized after clock and record the fluorescence intensity that interpretoscope reads detection reaction zone and reaction reference tape respectively, to detect reaction zone and reaction
The fluorescence intensity ratio of reference tape is ordinate, standard curve is drawn by abscissa of the standard antigen concentration of configuration.Using this
Scheme can be due to the error that the different band of the dissolving extent of reaction of the production difference of microfluidic card, sample and label etc. is come simultaneously
Reaction detection reaction zone and reaction reference tape, cause the fluorescence intensity of the two be while raised and lowered, with detect reaction zone with
The next shadow of this error band can then be effectively reduced as the ordinate of standard curve by reacting the ratio of reference tape, in addition can be prior
The standard curve making of correlation is good, it is stored in database and is transferred for detection immediately.
For above-mentioned X depending on immune response feature, the value according to different reaction X can be 3min, 5min or 10min
.
Above-mentioned record interpretoscope includes detection case and the operating desk installed in the positive facade of detection case, the operating desk and detection case
It is hinged；
Detection plate socket is provided with the front end of the detection case, the detection plate spigot is provided with detection plate conveyer
Structure, the detection plate conveying mechanism drive micro-fluidic plate to move forward and backward, and detection is provided with above the mobile route of the micro-fluidic plate
Mechanism, two-dimensional code scanning mechanism and testing agency's conveyer, testing agency's conveyer drive described testing agency or so
It is mobile；
The operating desk is provided with display screen, and processor and wireless communication module, processor point are provided with inside operating desk
Not with the detection plate conveying mechanism, testing agency, printer, display screen, two-dimensional code scanning mechanism, testing agency's conveyer
Connected with wireless communication module.
Using such scheme processor by the 2 D code information on the micro-fluidic plate of two-dimensional code scanning mechanism acquisition, and pass through
Wireless communication module transfers the every terms of information of micro-fluidic plate corresponding with the 2 D code information from cloud data center or the machine, such as
The information such as generation lot number, control information and the interpretation standard data of micro-fluidic plate, it is versatile.
The testing agency includes erecting bed, and fluorescence detector and excitation source are vertically arranged with the erecting bed, glimmering
Photodetector and excitation source are connected with the fluorescence signal input and excitation source control terminal of the processor respectively, the inspection
Mechanism conveyer drive installation platform is surveyed to move left and right.Using said structure, can be obtained by fluorescence detector on sense channel
Detect reaction zone and react the fluorescence intensity of reference tape, when being tested applied to multichannel micro-fluidic card, pass through fluorescence detector
Transport mechanism can be after the detection that fluorescence detector completes a sense channel, and driving fluorescence detector is moved to next detection
Above passage, next sense channel is detected.
Above-mentioned testing agency's conveyer includes left and right displacement motor, and the left and right displacement motor is controlled by motor driver,
The left and right displacement signal input part of the motor driver and the left and right displacement control terminal in the path clustering end group of the processor
Connection.Using said structure, testing agency's conveyer can drive testing agency to move left and right, and be easy to gather more detection informations.
Above-mentioned detection plate conveying mechanism includes moving forward and backward motor and detection plate clamping device, and the detection plate clamping device is set
Put and be connected in the detection plate spigot, the power transmission shaft for moving forward and backward motor with detection plate clamping device, the anteroposterior position
Move motor to be controlled by the motor driver, the road for moving forward and backward signal input part and the processor of the motor driver
Control terminal connection is moved forward and backward in the control terminal group of footpath.Using said structure, processor by move forward and backward motor can drive it is micro-
Stream control plate moves forward and backward, and is easy to the detection reaction zone to micro-fluidic plate and reaction reference area to carry out fluorescence intensity sweep test.
The above-mentioned motor and left and right displacement motor of moving forward and backward is stepper motor, and the motor driver drives for stepper motor
Dynamic device.Using said structure, stepper motor driver accurately the detection reaction zone to micro-fluidic plate and reaction reference area can be carried out
Fluorescence intensity sweep test, and it is logical fluorescence detector can also to be moved into next detection from a sense channel exactly
Road.
Beneficial effect：Using the beneficial effects of the invention are as follows no longer need to prepare to be coated with advance to have got around traditional miniflow
The bottleneck problem in detection technique is controlled, directly labelled antibody or antigen, sample are added dropwise in label area and sample respectively during detection
In product area, sample enters in label area the labelled antibody just added with the inside by microchannel or antigen directly reacts, and marks
The use of note antibody or antigen is not influenceed by technique for fixing, and detection is fast and reliable, additionally, due to without preparing fixation in advance, is somebody's turn to do
Method can be also used for some and be not suitable for carrying out the test event for pre-fixing labelled antibody or antigen in advance.
Brief description of the drawings
Fig. 1 is the structural representation of micro-fluidic plate；
Fig. 2 is the enlarged drawing in a portions in Fig. 1；
Fig. 3 is Fig. 1 A-A ' sectional views；
Fig. 4 is the enlarged drawing in b portions in Fig. 3；
Fig. 5 is the external structure schematic diagram of the record interpretoscope of embodiment 2；
Fig. 6 is the internal structure schematic diagram of the record interpretoscope of embodiment 2；
Fig. 7 is Fig. 6 side view；
Fig. 8 is the system architecture diagram of the record interpretoscope of embodiment 2.
Fig. 9 is the internal structure schematic diagram of the record interpretoscope of embodiment 3
Embodiment
With reference to embodiment and accompanying drawing, the invention will be further described.
Embodiment 1, as Figure 1-4, a kind of micro-fluidic plate, including core plate 1 and bottom plate 2, the core plate 1 are covered in institute
State on bottom plate 2, on the medial surface of the core plate 1 along the flow direction of liquid be sequentially provided with sample area 3, label area 4 and
Detection zone 7, the label area 4 are connected by the passage 18 that flows slowly with sample area 3, and the detection zone 7 passes through microchannel 17 and institute
State label area 4 to connect, be sequentially provided with detection reaction zone 5 along the flow direction of liquid on the detection zone 7 and reaction refers to
Band 6.
The bottom plate 2 is transparent panel, and the unhurried current passage 18 and microchannel 17 are respectively by between core plate 1 and bottom plate 2
Gap is formed, and miniflow siphon passage 19, the miniflow siphon passage are formed respectively between the bottom plate 2 and each detection zone 7
19 liquid feeding end is connected by the microchannel 17 with the label area 4, and the sample area 3 and mark are corresponded on the core plate 1
Ji Wu areas 4 are respectively equipped with well 9 and label adding hole 10.
Core plate 1 between the sample area 3 and label area 4 is provided with sample liquid deceleration area 11, the sample area 3 and sample liquid
Circulating direction in deceleration area 11 along liquid is respectively equipped with sample liquid deceleration boss 12, the sample area 3 and sample liquid deceleration area 11
Between be provided with sample liquid overflow step 21, liquid can enter sample liquid deceleration area 11 by the overflow of sample liquid overflow step 21, described slow
Circulation road 18 is recessed upwards respectively by the sample liquid deceleration area 11, the sample application zone 3, label area 4 and sample liquid deceleration area 11
Channel-shaped is formed, the cup depth of the sample application zone 3 is less than the cup depth of the sample liquid deceleration area 11, and the detection zone 7 enters
Liquid end is provided with mixed liquor flow control area 13, and depression forms channel-shaped, the mixing liquid stream upwards in the mixed liquor flow control area 13
The cup depth of fast control zone 13 is less than the cup depth in the label area 4, is provided with the mixed liquor flow control area 13
Mixed liquor deceleration boss, the core plate 1 at the outlet end rear of detection zone 7 are provided with waste collection pond 14, the miniflow rainbow
The outlet end for inhaling passage 19 is connected with the waste collection pond 14, and waste liquid deceleration boss 15, institute are provided with the waste collection pond 14
The diameter for stating the waste liquid deceleration boss 15 of the porch of waste collection pond 14 subtracts more than the waste liquid in the exit of waste collection pond 14
The diameter of fast boss 15.
From this figure it can be seen that it is respectively equipped with air-vent 16, institute on the core plate 1 of the both sides of sample liquid deceleration area 11
The core plate 1 for stating the both sides of detection zone 7 is provided with the bottom plate fixed station 20 of bar shaped, and two side lower parts of the bottom plate 2 pass through bonding
Agent is adhered on the corresponding bottom plate fixed station 20.
To accelerate detection speed, several label areas 4 can also be set on the core plate 1 of above-mentioned micro-fluidic plate simultaneously, it is different
Label area 4 connect different detection zones 7, sample enters different label areas 4 from sample area 3 by different passages
Afterwards, enter different detection zones 7 from different labelled antibody or antigen-reactive, so as in one-time detection same sample not
Same target antigen.
Embodiment 2, as viewed in figures 5-8, one kind record interpretoscope, including detection case c and installed in the positive facades of detection case c
Operating desk d, operating desk d is hinged with detection case c；
It is provided with the front end of the detection case c at detection plate socket e, the detection plate socket e and is provided with detection plate conveying
Mechanism f, the detection plate conveying mechanism f drive micro-fluidic plate to move forward and backward, and are provided with above the mobile route of the micro-fluidic plate
Testing agency g, two-dimensional code scanning mechanism h and testing agency conveyer i, testing agency conveyer i drive the detection
Mechanism g is moved left and right；
The operating desk d is provided with display screen, and processor and wireless communication module, processor are provided with inside operating desk d
Passed respectively with the detection plate conveying mechanism f, testing agency g, printer, display screen, two-dimensional code scanning mechanism h, testing agency
Device i is sent to be connected with wireless communication module.
The testing agency g includes being vertically arranged with fluorescence detector m and excitation source on erecting bed j, the erecting bed j
N, fluorescence detector m and excitation source n are connected with the fluorescence signal input and excitation source control terminal of the processor respectively,
Testing agency's conveyer i drive installation platforms j is moved left and right, and testing agency's conveyer i includes left and right displacement electricity
Machine, the left and right displacement motor are controlled by motor driver, left and right displacement signal input part and the processing of the motor driver
In the path clustering end group of device left and right displacement control terminal connection, the detection plate conveying mechanism f include move forward and backward motor p and
Detection plate clamping device q, detection plate clamping device q is arranged at the detection plate socket e, described to move forward and backward motor p's
Power transmission shaft t is connected with detection plate clamping device q, and the motor p that moves forward and backward is controlled by the motor driver, and the motor drives
The signal input part that moves forward and backward of dynamic device is connected with the control terminal that moves forward and backward in the path clustering end group of the processor, described
It is stepper motor to move forward and backward motor p and left and right displacement motor, and the motor driver is stepper motor driver.
Embodiment 3, as shown in figure 9, a kind of record interpretoscope, the present embodiment and the difference of embodiment 2 are：It is described
Testing agency g includes erecting bed j, and camera k and the light source s that takes pictures, camera k and light of taking pictures are vertically arranged with erecting bed j
Source s is connected with the image input and illuminance control terminal of the processor respectively.
The specific workflow of embodiment 2 and embodiment 3 is as follows：Surveyed when being added on the micro-fluidic plate into embodiment 1
After test liquid, micro-fluidic plate is inserted in tester at detection plate socket e, processor is scanned by two-dimensional code scanning mechanism h
2 D code information on micro-fluidic plate, and according to 2 D code information, adjusted by wireless communication module from cloud data center or the machine
Information corresponding to the micro-fluidic plate is taken, the information includes interpretation mark data of production batch, control information and sense channel etc.,
Processor judges whether the micro-fluidic plate can use according to generation batch, if can not use, processor is by showing screen display
Show micro-fluidic plate outdated information, if can use, driving testing agency g detects to the regional of micro-fluidic plate.
When one of region is as after the completion of reacting the detection of reference tape 6, processor drives left and right displacement according to control information
Motor and the movement for moving forward and backward motor p, testing agency g is moved to the top of detection reaction zone 5 and be scanned detection.
Embodiment 4, a kind of sample-adding detection method twice based on microflow control technique, including it is micro-fluidic described in embodiment 1
Record interpretoscope described in plate and embodiment 2, is detected according to the following steps：
The first step makes standard curve：Standard antigen of the configuration with concentration gradient, by several times in the mark of the micro-fluidic plate
Remember after fluorescein labelled antibody is added dropwise in thing adding hole 10, then toward the standard antigen that various concentrations are added dropwise in well 9 by several times,
Interval X minutes after using record interpretoscope analyze respectively detection reaction zone 5 (being recorded as T areas for convenience of description) and react reference tape
The fluorescence intensity of 6 (being recorded as C areas for convenience of description), specific method are to insert micro-fluidic plate by the socket on instrument, two
The 2 D code information on the micro-fluidic plate of code sweep mechanism h scannings is tieed up, processor driving testing agency g obtains T areas and C fluorescence is strong
Degree, the fluorescence intensity ratio using T areas and C areas be T/C as ordinate, by abscissa of the standard antigen concentration of configuration draw standard
Curve；
Second step tests and analyzes：Fluorescein labelled antibody is added drop-wise to by the label area 4 by label adding hole 10
It is interior, then sample liquid is added drop-wise in the sample area 3 by the well 9, is utilized after the X minutes of interval and records interpretoscope analysis
T areas and the fluorescence intensity in C areas, the concentration of target antigen in sample liquid is then calculated using standard curve.
In the detection method, the target substance that the material that is first added dropwise is alternatively antigen and needs to determine is anti-in sample liquid
Body, then standard curve, detection need to be made by ordinate of T/C values using standard antibody concentration as abscissa when making standard curve
When fluorescein labelled antigen is first added drop-wise to label area 4, then sample liquid is added dropwise in sample area 3, T areas is read after being spaced X minutes
With the fluorescence intensity in C areas, and according to standard curve calculate sample liquid in target antibody concentration.
Embodiment 5, a kind of sample-adding detection method twice based on microflow control technique, the present embodiment and the difference of embodiment 4
Part is：Record interpretoscope used is the record interpretoscope that embodiment 3 provides, and the record interpretoscope is micro-fluidic for analyzing
The shade of plate different zones, labelled antibody used are colloidal gold labeled monoclonal antibody, are spaced the glue in X minute post analysis T areas and C areas
Body gold color intensity, it is specially：Micro-fluidic plate is inserted by the socket on instrument, two-dimensional code scanning mechanism h scannings are micro-fluidic
2 D code information on plate, processor driving testing agency obtains the image information in T areas and C areas, and image information is divided
Analysis, the image processing method use with published Application No. CN201010512048.2, it is entitled " one kind by image at
Identical method carries out image procossing in the method that reason carries out immune chromatography result analysis ", and the comentropy for calculating two regions is big
It is small, using the comentropy ratio in T areas and C areas as ordinate, standard curve is drawn by abscissa of the standard antigen concentration of configuration, is surveyed
The T/C values of sample liquid will be tested during examination compared with standard curve so as to calculating the concentration of target antigen in sample liquid, controller c
Driving printer prints test data.
Method provided by the invention can be used for LH, HCG etc. quick detection, typically add when labelled antibody or antigen is added dropwise
Enter amount less than 5 microlitres, the antibody so first added will not flow, and general about 35 microlitres of addition is anti-to ensure when testing sample is added dropwise
Should fully, detection it is more accurate, method provided by the invention can also be used for the micro-fluidic plate with multiple sense channels while detect
Different target antigen or antibody in same sample.
Finally it should be noted that foregoing description is only the preferred embodiments of the present invention, the ordinary skill people of this area
Member on the premise of without prejudice to present inventive concept and claim, can make table as multiple types under the enlightenment of the present invention
Show, such conversion is each fallen within protection scope of the present invention.
Claims (9)
- A kind of 1. sample-adding detection method twice based on microflow control technique, it is characterised in that：Including micro-fluidic plate and record interpretation Instrument, detected according to the following steps：Labelled antibody or antigen are added dropwise in the label area (4) of the micro-fluidic plate first, connect The sample area (3) that sample liquid is dropped in micro-fluidic plate, be spaced after X minutes and read and analyze micro-fluidic upper inspection using recording interpretoscope Survey the testing result of area (7).
- 2. the sample-adding detection method according to claim 1 twice based on microflow control technique, it is characterised in that：The miniflow Control plate includes core plate (1) and bottom plate (2), and the core plate (1) is covered on the bottom plate (2), on the medial surface of the core plate (1) Sample area (3), label area (4) and the detection zone (7) being sequentially communicated, the core plate (1) are provided with along the flow direction of sample liquid Upper counter sample area (3) and label area (4) are respectively equipped with well (9) and label adding hole (10), the detection zone (7) On along the flow direction of sample liquid be sequentially provided with detection reaction zone (5) and react reference tape (6).
- 3. the sample-adding detection method according to claim 2 twice based on microflow control technique, it is characterised in that：The reading And the method for analyzing the result of detection zone (7) on micro-fluidic plate is：The labelled antibody or antigen be fluorescein labelled antibody or Antigen, detection reaction zone (5) is read respectively and reacts the fluorescence intensity of reference tape (6), and according to standard using interpretoscope is recorded Curve calculates the concentration of target substance in sample liquid.
- 4. the sample-adding detection method according to claim 3 twice based on microflow control technique, it is characterised in that：The standard The preparation method of curve is：Standard antigen of the configuration with concentration gradient, the label adding hole on the micro-fluidic plate (10) after fluorescein labelled antibody is added dropwise in, the standard antigen of various concentrations is added dropwise in well (9), is spaced profit after X minutes With record interpretoscope respectively read detection reaction zone (5) and react reference tape (6) fluorescence intensity, with detect reaction zone (5) and The fluorescence intensity ratio for reacting reference tape (6) is ordinate, standard curve is drawn by abscissa of the standard antigen concentration of configuration.
- 5. the sample-adding detection method twice based on microflow control technique according to claim any one of 1-4, it is characterised in that： The record interpretoscope includes detection case (c) and the operating desk (d) installed in the positive facade of detection case (c), the operating desk (d) and inspection Measuring tank (c) is hinged；Detection plate socket (e) is provided with the front end of the detection case (c), it is defeated that detection plate socket (e) place is provided with detection plate Mechanism (f) is sent, the detection plate conveying mechanism (f) drives micro-fluidic plate to move forward and backward, above the mobile route of the micro-fluidic plate Testing agency (g), two-dimensional code scanning mechanism (h) and testing agency's conveyer (i) are provided with, testing agency's conveyer (i) testing agency (g) is driven to move left and right；The operating desk (d) is provided with display screen, and processor and wireless communication module, processor are provided with inside operating desk (d) Respectively with the detection plate conveying mechanism (f), testing agency (g), printer, display screen, two-dimensional code scanning mechanism (h), detection Mechanism conveyer (i) connects with wireless communication module.
- 6. the sample-adding detection method according to claim 5 twice based on microflow control technique, it is characterised in that：The detection Mechanism (g) includes erecting bed (j), and fluorescence detector (m) and excitation source (n), fluorescence are vertically arranged with the erecting bed (j) Detector (m) and excitation source (n) are connected with the fluorescence signal input and excitation source control terminal of the processor respectively, institute Testing agency's conveyer (i) drive installation platform (j) is stated to move left and right.
- 7. the micro-fluidic record interpretoscope of self-absorption multichannel according to claim 5, it is characterised in that：The testing agency passes Device (i) is sent to include left and right displacement motor, the left and right displacement motor is controlled by motor driver, the left and right position of the motor driver Shifting signal input is connected with the left and right displacement control terminal in the path clustering end group of the processor.
- 8. the micro-fluidic record interpretoscope of self-absorption multichannel according to claim 7, it is characterised in that：The detection plate conveying Mechanism (f) includes moving forward and backward motor (p) and detection plate clamping device (q), and the detection plate clamping device (q) is arranged on the inspection Drafting board socket (e) place, the power transmission shaft (t) for moving forward and backward motor (p) are connected with detection plate clamping device (q), described front and rear Displacement motor (p) is controlled by the motor driver, and the motor driver moves forward and backward signal input part and the processing Control terminal connection is moved forward and backward in the path clustering end group of device.
- 9. the micro-fluidic record interpretoscope of self-absorption multichannel according to claim 8, it is characterised in that：It is described to move forward and backward electricity Machine (p) and left and right displacement motor are stepper motor, and the motor driver is stepper motor driver.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109490535A (en) * | 2018-10-22 | 2019-03-19 | 湖北洁源检测有限公司 | A kind of multi-channel synchronous immunochromatography detection system and detection method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101102847A (en) * | 2005-01-18 | 2008-01-09 | 生物概念股份有限公司 | Cell separation using microchannel having patterned posts |
| CN103582816A (en) * | 2011-04-06 | 2014-02-12 | 奥索临床诊断有限公司 | Assay device having rhombus-shaped projections |
| CN205199533U (en) * | 2015-12-17 | 2016-05-04 | 广州万孚生物技术股份有限公司 | Micro -fluidic chip for detection |
| CN106563518A (en) * | 2016-11-08 | 2017-04-19 | 常州锐德医疗科技有限公司 | Micro-fluidic chip for rapidly detecting three subtypes of bladder cancer and manufacturing method and use method thereof |
| CN106662522A (en) * | 2014-06-18 | 2017-05-10 | 斯堪的纳维亚微生物制剂公司 | A microfluidic detection system and a microfluidic cartridge |
-
2017
- 2017-08-10 CN CN201710681307.6A patent/CN107462735B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101102847A (en) * | 2005-01-18 | 2008-01-09 | 生物概念股份有限公司 | Cell separation using microchannel having patterned posts |
| CN103582816A (en) * | 2011-04-06 | 2014-02-12 | 奥索临床诊断有限公司 | Assay device having rhombus-shaped projections |
| CN106662522A (en) * | 2014-06-18 | 2017-05-10 | 斯堪的纳维亚微生物制剂公司 | A microfluidic detection system and a microfluidic cartridge |
| CN205199533U (en) * | 2015-12-17 | 2016-05-04 | 广州万孚生物技术股份有限公司 | Micro -fluidic chip for detection |
| CN106563518A (en) * | 2016-11-08 | 2017-04-19 | 常州锐德医疗科技有限公司 | Micro-fluidic chip for rapidly detecting three subtypes of bladder cancer and manufacturing method and use method thereof |
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| CN109490535A (en) * | 2018-10-22 | 2019-03-19 | 湖北洁源检测有限公司 | A kind of multi-channel synchronous immunochromatography detection system and detection method |
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