CN1922989B - Artificial seedling breeding method for haliotis asinine - Google Patents
Artificial seedling breeding method for haliotis asinine Download PDFInfo
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- CN1922989B CN1922989B CN2006101152717A CN200610115271A CN1922989B CN 1922989 B CN1922989 B CN 1922989B CN 2006101152717 A CN2006101152717 A CN 2006101152717A CN 200610115271 A CN200610115271 A CN 200610115271A CN 1922989 B CN1922989 B CN 1922989B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention relates to an artificial seeding method of ear abalone. Wherein, it comprises cultivating benthic diatom, breeding ear abalone, and cultivating young abalone, while the first step adds seawater into seed pool, filters benthic diatom, and lays bait plate, to cultivate the benthic diatom on the plate; and the second step selects healthy adult abalone to train and grow, and artificially induces the parent abalones, to lay eggs, and artificially fecundate in seawater, then selecting and breeding the young ones; and the third step lays bait plate adhered with benthic diatom into seeding pool, to adhere the young one on the bait plate, and remove it to network bank when grows to older one.
Description
Technical field
The present invention relates to a kind of artificial raise seedling method of abalone new varieties, i.e. the artificial raise seedling method of ear Bao.
Background technology
Abalone belongs to sea mollusk, is original gastropod such as low, about 100 kinds of abalones that in world ocean, distributing, and great majority are distributed in the temperate zone, and minority is distributed in the torrid zone and frigid zone.Abalone-shaped is like the ear shape, and its muscle of foot prosperity relies on that this strong muscle of foot is adsorbed on that trend is unimpeded, among the seawater clarification, higher, the weed-filled rock of salinity bottom reef dish crack.Liking concealinging oneself by day and march by night, is food with the algae, especially happiness food sea-tangle, agar etc.
The abalone flavor is extremely delicious, nutritious, is described as " first of the seafood delights eight delicacies ".According to one's analysis, the dried Bao of every hectogram contains protein 40 gram, sugar 33.7 grams, fat 0.9 gram, also contains multiple nutrients materials such as calcium, phosphorus, iron, iodine and vitamin, has the function that enriching yin and nourishing kidney, menstruation regulating benefit essence, flat liver make eye bright.Its shell Chinese medicine also is the good medicine of the multiple disease of treatment the shell of seaear, therefore becomes domestic and international market high-grade seafood delights good merchantable brand salable.
The large-scale economic Bao that has commodity value in the abalone of China has haliotis discus hannai Ino, Haliotis diversicolor (haliotis diversicolor Reeve), sheep Bao and Er Bao.Ear Bao (Haliotis asinina) is commonly called as donkey ear Bao, is a kind of marine products economic shellfish, has high edibility and medical value, and it mainly is distributed in the torrid zone and ocean, subtropics.
Because the international source of goods rareness of abalone, for increasing the output of abalone, the world respectively produces the Bao area and carries out in succession and propagate artificially, the artificial propagation techniques of haliotis discus hannai Ino, Haliotis diversicolor (haliotis diversicolor Reeve) is quite perfect at present, and dropped among the practical application, accumulated many experiences about artificial abalone breeding and breed.But the artificial propagation techniques of ear Bao still fails to grasp and report at present.
Summary of the invention
The objective of the invention is still to fail to grasp and the deficiencies in the prior art are provided at present the artificial raise seedling method of a kind of ear Bao at the artificial propagation techniques of ear Bao.
The artificial raise seedling method of the ear Bao that the present invention is designed comprises the cultivating process of incubation, ear Bao hatching cultivating process and the young Bao of benthic diatom.
1, the incubation of benthic diatom cleans up the bait plate, soaks with sodium hydroxide solution, rinses well standby then; Add seawater in inoculation in the pond, the algae kind is filtered in the pond be mixed, the bait plate is layed in the pond, allow the algae kind, will add the nutritive salt of nitrogenous, phosphorus, iron therebetween,, allow the algae kind can breed growth better with the element that has additional nutrients in the breeding of bait plate upper surface.
2, ear Bao hatching cultivating process screens healthy adult Bao and is placed on and carries out artificial domestication among the close Bao Chi and accelerate the ripening; The male and female parent Bao fully ripe sexual gland carries out the artificial induction respectively, it is laid eggs or arrange essence, carries out artificial insemination then, allows fertilized egg hatch in seawater; The trochophore that hatching is obtained filters out the strong individuality of vitality and cultivates, and allows it grow the larva that crawls.
3, the cultivating process of young Bao will be put into nursery pond after will rinsing well with the bait plate of benthic diatom, allow the larva that crawls be attached on the bait plate and cultivate, in nursery pond, add the nutritive salt of nitrogenous, phosphorus, iron therebetween,, benthic diatom is grown continuously with the element that has additional nutrients.If the bait deficiency can be inserted with new bait plate, or throw something and feed flat algae or fragrant plant mentioned in ancient texts slurry.
When crawling larval growth behind the young Bao, it is peeled off to move in the net cage cultivate.
The artificial raise seedling method of ear Bao provided by the present invention can improve kind of Bao Zhiliang and smart ovum vigor, fertilization rate is improved, children Bao Zhi Bao survival rate height, Bao Miao does not take off plate, large-scale Bao Miao death can not appear, and can carry out annual ear Bao and grow seedlings, save production cost, improve production efficiency.
Embodiment
Below the present invention is described in further detail.
The artificial raise seedling method of the ear Bao that the present invention is designed comprises the cultivating process of incubation, ear Bao hatching cultivating process and the young Bao of benthic diatom.
1, the incubation of benthic diatom
Choose shellfish shell string, polyethylene film as the bait plate, the bait plate is cleaned up the back soaked 1～2 day, rinse well standby then with 0.5% sodium hydroxide solution; In the inoculation pond, add filtered seawater, the algae kind filtered in the pond be mixed, then the bait plate is layed in the pond in the imbricate mode, allow the algae kind breed at bait plate upper surface, and regulate illuminance between 2000～10000 luxs, to add the nutritive salt of nitrogenous, phosphorus, iron therebetween, to increase nitrogen, phosphorus, iron nutritive element, the ratio that contains nitrogen, phosphorus, iron in the nutritive salt that is added is 1: 0.02: 0.02, makes benthic diatom can breed growth better.Described algae kind is boat-shaped algae, avette algae, rhombus algae and Closterium.
2, ear Bao hatching cultivating process
The individual healthy adult Bao of screening greater than 3cm, after sterilization, be placed on and carry out artificial domestication among the close Bao Chi and accelerate the ripening, in the process with fragrant plant mentioned in ancient texts, sea-tangle or Eucheuma are carrier, adding nutrition fortifier soaks, the adult Bao of throwing something and feeding then, increase the nutrition of adult Bao, to increase kind of Bao Zhiliang and smart ovum vigor, fertilization rate is improved, said nutrition fortifier is by vegetable protein, bata-carotene, polysaccharides, acid and gamma-linolenic, vitamin is formed, the ratio of wherein counting by weight percentage each component is: vegetable protein 70～99%, bata-carotene 0.1～0.2%, polysaccharides 0.018～0.022%, acid and gamma-linolenic 0.8～1.3%, vitamin 0.004～0.008%); When the adult Bao carries out the artificial induction to the close Bao of the fully ripe male and female of sexual gland respectively through the artificial domestication and the back of accelerating the ripening, it is laid eggs or arrange essence, its abductive approach is that ocyodinic is expelled in the close Bao body, said ocyodinic is 40～45% solubility calcium salting liquid; Finish in smart back 1 hour in the row of laying eggs and to carry out artificial insemination, treat that fertilized egg sinks to the bottom the back and washes ovum 8～9 times with the precipitation method, allow fertilized egg in proportion is 1.022～1.023 seawater, hatch; The trochophore that hatching is obtained filters out the strong individuality of vitality and cultivates, and cultivating density is 50～300/liter, every two hours changes water once in cultivating process, and quantity of exchanged water allows trochophore grow the larva that crawls for cultivating 1/3～1/2 of water body.The sterilization seawater that the artificial induction's method that is adopted also has method of making herbs dry gradually in the shade, heat shock method, ultraviolet irradiation, injection to filter arrives head, enteric ganglia or the pedal ganglion of close Bao, and the mode of employing is one or more combinations in the above method.
3, the cultivating process of young Bao
Be no less than 20～30 square metres injected volume by the every square metre of area of collecting seedling and put into nursery pond rinsing the back well with the bait plate of benthic diatom, allow the larva that crawls be attached on the bait plate, in order to impel the larva that crawls attached on the bait plate, in the cultivation water body, add adhesive agent, said adhesive agent is 42～48mmol/LKCL solution, for larva is adhered to evenly, can weaken light, and hand over and be inverted the bait plate; Carry out flowing water and cultivate after the week of collecting seedling, day quantity of exchanged water is 1/2～1 times of the cultivation water body, keeps water temperature between 18～25 ℃, salinity is more than 22.25, dissolved oxygen content is more than 5 mg/litre, and pH value is between 7.9～8.2, and intensity of illumination is more than 1000 luxs; Collect seedling behind the two weeks, add the nutritive salt of nitrogenous, phosphorus, iron in nursery pond, with the element that has additional nutrients, the ratio that contains nitrogen, phosphorus, iron in the nutritive salt that is added is 1: 0.02: 0.02, and benthic diatom is grown continuously.If the bait deficiency can be inserted with new bait plate, or throw something and feed flat algae or fragrant plant mentioned in ancient texts slurry.When crawling larval growth behind the young Bao, it is peeled off to move in the net cage cultivate, cultivating density is 5000～6000/meter
2, the bait feeding amount is 4～5% of a young Bao body weight.The stripping means of described young Bao: after (1) soaked 3～5 minutes with the own ester sea water solution of 1% carbamic acid, shake bait plate made it to come off; (2) peel off with the anesthesia of 2～3% alcohol; (3) peel off with FQ～420 anesthesia; (4) water impact peel.
Claims (4)
1. the artificial raise seedling method of an ear Bao comprises the incubation of benthic diatom, the cultivating process that the ear Bao hatches cultivating process and young Bao, it is characterized in that:
1) incubation of described benthic diatom is to choose shellfish shell string, polyethylene film as the bait plate, the bait plate is cleaned up the back soaked 1～2 day with 0.5% sodium hydroxide solution, rinses well standby then; In the inoculation pond, add filtered seawater, the algae kind filtered in the pond be mixed, then the bait plate is layed in the pond in the imbricate mode, allow the algae kind breed at bait plate upper surface, and regulate illuminance between 2000～10000 luxs, will add the nutritive salt of nitrogenous, phosphorus, iron therebetween, the ratio that contains nitrogen, phosphorus, iron in the nutritive salt that is added is 1: 0.02: 0.02;
2), described ear Bao hatching cultivating process is the individual healthy adult Bao greater than 3cm of screening, after sterilization, be placed on and carry out artificial domestication among the close Bao Chi and accelerate the ripening, in the process with fragrant plant mentioned in ancient texts, sea-tangle or Eucheuma are carrier, adding nutrition fortifier soaks, the adult Bao of throwing something and feeding then, said nutrition fortifier is by vegetable protein, bata-carotene, polysaccharides, acid and gamma-linolenic, vitamin is formed, the ratio of wherein counting by weight percentage each component is: vegetable protein 70～99%, bata-carotene 0.1～0.2%, polysaccharides 0.018～0.022%, acid and gamma-linolenic 0.8～1.3%, vitamin 0.004～0.008%); When the adult Bao carries out the artificial induction to the close Bao of the fully ripe male and female of sexual gland respectively through the artificial domestication and the back of accelerating the ripening, it is laid eggs or arrange essence, its abductive approach is that ocyodinic is expelled in the close Bao body, said ocyodinic is 40～45% solubility calcium salting liquid; Finish in smart back 1 hour in the row of laying eggs and to carry out artificial insemination, treat that fertilized egg sinks to the bottom the back and washes ovum 8～9 times with the precipitation method, allow fertilized egg in proportion is 1.022～1.023 seawater, hatch; The trochophore that hatching is obtained filters out the strong individuality of vitality and cultivates, and cultivating density is 50～300/liter, every two hours changes water once in cultivating process, and quantity of exchanged water is for cultivating 1/3～1/2 of water body, allow trochophore grow the larva that crawls;
3), the cultivating process of described young Bao is to be no less than 20～30 square metres injected volume by the every square metre of area of collecting seedling and to put into nursery pond rinsing the back well with the bait plate of benthic diatom, allow the larva that crawls be attached on the bait plate, in the cultivation water body, add adhesive agent, said adhesive agent is 42～48mmol/LKCL solution, can weaken light, be inverted the bait plate in the process; Carry out flowing water and cultivate after the week of collecting seedling, day quantity of exchanged water is 1/2～1 times of the cultivation water body, keeps water temperature between 18～25 ℃, salinity is more than 22.25, dissolved oxygen content is more than 5 mg/litre, and pH value is between 7.9～8.2, and intensity of illumination is more than 1000 luxs; Collect seedling behind the two weeks, add the nutritive salt of nitrogenous, phosphorus, iron in nursery pond, the ratio that contains nitrogen, phosphorus, iron in the nutritive salt that is added is 1: 0.02: 0.02; When crawling larval growth behind the young Bao, it is peeled off to move in the net cage cultivate, cultivating density is 5000～6000/meter
2, the bait feeding amount is 4～5% of a young Bao body weight.
2. according to the artificial raise seedling method of the said ear Bao of claim 1, it is characterized in that: described algae kind is boat-shaped algae, avette algae, rhombus algae and Closterium.
3. according to the artificial raise seedling method of the said ear Bao of claim 1, it is characterized in that: the sterilization seawater that the artificial induction's method that is adopted also has method of making herbs dry gradually in the shade, heat shock method, ultraviolet irradiation, injection to filter arrives head, enteric ganglia or the pedal ganglion of close Bao, and the mode of employing is one or more combinations in the above method.
4. according to the artificial raise seedling method of the said ear Bao of claim 1, it is characterized in that: the stripping means of described young Bao: after (1) soaked 3～5 minutes with the own ester sea water solution of 1% carbamic acid, shake bait plate made it to come off; (2) peel off with the anesthesia of 2～3% alcohol; (3) peel off with FQ～420 anesthesia; (4) water impact peel.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2006101152717A CN1922989B (en) | 2006-08-13 | 2006-08-13 | Artificial seedling breeding method for haliotis asinine |
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| CN2006101152717A CN1922989B (en) | 2006-08-13 | 2006-08-13 | Artificial seedling breeding method for haliotis asinine |
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| CN1922989A CN1922989A (en) | 2007-03-07 |
| CN1922989B true CN1922989B (en) | 2011-09-21 |
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| CN2006101152717A Expired - Fee Related CN1922989B (en) | 2006-08-13 | 2006-08-13 | Artificial seedling breeding method for haliotis asinine |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101331861B (en) * | 2008-07-29 | 2010-12-22 | 中国海洋大学 | Seashell spawning method using chemo-induction |
| CN101904308B (en) * | 2010-07-26 | 2013-07-31 | 大连海宝渔业有限公司 | Parent haliotis discus hannai mature-promoting method capable of realizing early breeding |
| CN102239798B (en) * | 2011-04-13 | 2012-07-25 | 福建省水产研究所 | Method for massively, quickly and directionally culturing high-quality young abalone feed such as Cocconeis |
| CN103535297B (en) * | 2013-10-08 | 2015-04-22 | 威海长青海洋科技股份有限公司 | Young abalone bait feeding method |
| CN103975880B (en) * | 2014-05-12 | 2015-12-02 | 福建港德水产有限公司 | A kind of method that hippocampus and abalone and fragrant plant mentioned in ancient texts are raised together with |
| CN107211932A (en) * | 2017-05-30 | 2017-09-29 | 赵玉明 | A kind of abalone offspring seed cultivation method |
| CN115956525A (en) * | 2023-02-01 | 2023-04-14 | 山东大学 | Healthy cultivation method of selenium-enriched abalone |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1191068A (en) * | 1997-11-04 | 1998-08-26 | 赵洪恩 | Artificial abalone fry culturing process |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1191068A (en) * | 1997-11-04 | 1998-08-26 | 赵洪恩 | Artificial abalone fry culturing process |
Non-Patent Citations (4)
| Title |
|---|
| 黄勃,王小兵,刘杰俊.耳鲍人工养殖的可行性研究.海洋科学26 7.2002,26(7),40,41. |
| 黄勃,王小兵,刘杰俊.耳鲍人工养殖的可行性研究.海洋科学26 7.2002,26(7),40,41. * |
| 黄勃.杂色鲍半自动工厂化养殖技术.渔业现代化2003 3.2003,2003(3),16,17. |
| 黄勃.杂色鲍半自动工厂化养殖技术.渔业现代化2003 3.2003,2003(3),16,17. * |
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Granted publication date: 20110921 Termination date: 20120813 |