CN1856579A - 使用热稳定酶合成多核苷酸的方法 - Google Patents

使用热稳定酶合成多核苷酸的方法 Download PDF

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Publication number
CN1856579A
CN1856579A CNA2004800274720A CN200480027472A CN1856579A CN 1856579 A CN1856579 A CN 1856579A CN A2004800274720 A CNA2004800274720 A CN A2004800274720A CN 200480027472 A CN200480027472 A CN 200480027472A CN 1856579 A CN1856579 A CN 1856579A
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China
Prior art keywords
cell
cells
polynucleotide
kit
cloning vector
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Pending
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CNA2004800274720A
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English (en)
Chinese (zh)
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玛乔丽·F.·奥莱克夏克
道格拉斯·L.·克劳福德
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University of Missouri Columbia
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University of Missouri Columbia
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Publication of CN1856579A publication Critical patent/CN1856579A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
CNA2004800274720A 2003-09-23 2004-09-23 使用热稳定酶合成多核苷酸的方法 Pending CN1856579A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US50530003P 2003-09-23 2003-09-23
US60/505,300 2003-09-23

Publications (1)

Publication Number Publication Date
CN1856579A true CN1856579A (zh) 2006-11-01

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CNA2004800274720A Pending CN1856579A (zh) 2003-09-23 2004-09-23 使用热稳定酶合成多核苷酸的方法

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US (2) US20050064490A1 (https=)
EP (1) EP1664347A1 (https=)
JP (1) JP2007506430A (https=)
CN (1) CN1856579A (https=)
AU (1) AU2004276790A1 (https=)
CA (1) CA2535922A1 (https=)
WO (1) WO2005030995A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109477127A (zh) * 2016-03-14 2019-03-15 R基因股份有限公司 超热稳定的赖氨酸-突变型ssDNA/RNA连接酶
CN113481174A (zh) * 2021-07-01 2021-10-08 温州医科大学 核酸连接酶

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US7445926B2 (en) 2002-12-30 2008-11-04 The Regents Of The University Of California Fluid control structures in microfluidic devices
US7799553B2 (en) 2004-06-01 2010-09-21 The Regents Of The University Of California Microfabricated integrated DNA analysis system
CN101073002B (zh) 2004-09-15 2012-08-08 英特基因有限公司 微流体装置
EP1979079A4 (en) 2006-02-03 2012-11-28 Integenx Inc MICROFLUIDIC DEVICES
US7766033B2 (en) 2006-03-22 2010-08-03 The Regents Of The University Of California Multiplexed latching valves for microfluidic devices and processors
US8841116B2 (en) 2006-10-25 2014-09-23 The Regents Of The University Of California Inline-injection microdevice and microfabricated integrated DNA analysis system using same
US20110039303A1 (en) 2007-02-05 2011-02-17 Stevan Bogdan Jovanovich Microfluidic and nanofluidic devices, systems, and applications
US8454906B2 (en) 2007-07-24 2013-06-04 The Regents Of The University Of California Microfabricated droplet generator for single molecule/cell genetic analysis in engineered monodispersed emulsions
KR20110030415A (ko) 2008-01-22 2011-03-23 인터젠엑스 인크. 만능 샘플 제조 시스템 및 집적 분석 시스템에서의 용도
KR20110111449A (ko) 2008-12-31 2011-10-11 인터젠엑스 인크. 미세유체 칩을 갖는 기구
US8685648B2 (en) * 2009-02-03 2014-04-01 Biohelix Corporation Endonuclease-enhanced helicase-dependent amplification
CN102459565A (zh) 2009-06-02 2012-05-16 尹特根埃克斯有限公司 具有隔膜阀的流控设备
US8584703B2 (en) 2009-12-01 2013-11-19 Integenx Inc. Device with diaphragm valve
US8512538B2 (en) 2010-05-28 2013-08-20 Integenx Inc. Capillary electrophoresis device
EP2606154B1 (en) 2010-08-20 2019-09-25 Integenx Inc. Integrated analysis system
WO2012024657A1 (en) 2010-08-20 2012-02-23 IntegenX, Inc. Microfluidic devices with mechanically-sealed diaphragm valves
EP2611922A1 (en) 2010-08-31 2013-07-10 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways through protease manipulation
AU2014306074B2 (en) 2013-08-05 2018-08-30 Greenlight Biosciences, Inc. Engineered proteins with a protease cleavage site
BR112017020690A2 (pt) 2015-03-30 2018-06-26 Greenlight Biosciences Inc produção livre de células de ácido ribonucleico
CA3020312A1 (en) * 2016-04-06 2017-10-12 Greenlight Biosciences, Inc. Cell-free production of ribonucleic acid
EP3565892A4 (en) 2017-01-06 2020-10-07 Greenlight Biosciences, Inc. Cell-free production of sugars
KR102894284B1 (ko) 2017-10-11 2025-12-03 그린라이트 바이오사이언시스, 아이엔씨. 뉴클레오시드 트리포스페이트 및 리보핵산 생산을 위한 방법 및 조성물

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US5374553A (en) * 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US5283173A (en) * 1990-01-24 1994-02-01 The Research Foundation Of State University Of New York System to detect protein-protein interactions
US5352778A (en) * 1990-04-26 1994-10-04 New England Biolabs, Inc. Recombinant thermostable DNA polymerase from archaebacteria
US5756334A (en) * 1990-04-26 1998-05-26 New England Biolabs, Inc. Thermostable DNA polymerase from 9°N-7 and methods for producing the same
CA2092317A1 (en) * 1990-09-28 1992-03-29 David H. Gelfand Purified thermostable nucleic acid polymerase enzyme from thermosipho africanus
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CA2159081C (en) * 1993-03-24 2000-11-21 David Mead Dinucleotide restriction endonuclease preparations and methods of use
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US6306588B1 (en) * 1997-02-07 2001-10-23 Invitrogen Corporation Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof
DE69836482T2 (de) * 1997-07-25 2007-09-27 Agene Research Institute, Co., Ltd., Kamakura Menschliches recq4 gen, das für eine helikase kodiert
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109477127A (zh) * 2016-03-14 2019-03-15 R基因股份有限公司 超热稳定的赖氨酸-突变型ssDNA/RNA连接酶
CN113481174A (zh) * 2021-07-01 2021-10-08 温州医科大学 核酸连接酶
CN113481174B (zh) * 2021-07-01 2022-08-19 温州医科大学 核酸连接酶

Also Published As

Publication number Publication date
US20050064490A1 (en) 2005-03-24
WO2005030995A1 (en) 2005-04-07
US20060252083A1 (en) 2006-11-09
CA2535922A1 (en) 2005-04-07
JP2007506430A (ja) 2007-03-22
EP1664347A1 (en) 2006-06-07
AU2004276790A1 (en) 2005-04-07

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Open date: 20061101