JP2007506430A - 耐熱性酵素を使用するポリヌクレオチド合成方法 - Google Patents

耐熱性酵素を使用するポリヌクレオチド合成方法 Download PDF

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Publication number
JP2007506430A
JP2007506430A JP2006528170A JP2006528170A JP2007506430A JP 2007506430 A JP2007506430 A JP 2007506430A JP 2006528170 A JP2006528170 A JP 2006528170A JP 2006528170 A JP2006528170 A JP 2006528170A JP 2007506430 A JP2007506430 A JP 2007506430A
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cell
polynucleotide
kit
cloning vector
reaction mixture
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JP2007506430A5 (https=
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マージョリー エフ オルクシアック
ダグラス エル クローフォード
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ユニヴァーシティー オブ ミズーリ
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
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  • Microbiology (AREA)
  • Molecular Biology (AREA)
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  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2006528170A 2003-09-23 2004-09-23 耐熱性酵素を使用するポリヌクレオチド合成方法 Abandoned JP2007506430A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US50530003P 2003-09-23 2003-09-23
PCT/US2004/031267 WO2005030995A1 (en) 2003-09-23 2004-09-23 Methods of synthesizing polynucleotides using thermostable enzymes

Publications (2)

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JP2007506430A true JP2007506430A (ja) 2007-03-22
JP2007506430A5 JP2007506430A5 (https=) 2007-11-15

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JP2006528170A Abandoned JP2007506430A (ja) 2003-09-23 2004-09-23 耐熱性酵素を使用するポリヌクレオチド合成方法

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US (2) US20050064490A1 (https=)
EP (1) EP1664347A1 (https=)
JP (1) JP2007506430A (https=)
CN (1) CN1856579A (https=)
AU (1) AU2004276790A1 (https=)
CA (1) CA2535922A1 (https=)
WO (1) WO2005030995A1 (https=)

Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8286665B2 (en) 2006-03-22 2012-10-16 The Regents Of The University Of California Multiplexed latching valves for microfluidic devices and processors
US8388908B2 (en) 2009-06-02 2013-03-05 Integenx Inc. Fluidic devices with diaphragm valves
US8420318B2 (en) 2004-06-01 2013-04-16 The Regents Of The University Of California Microfabricated integrated DNA analysis system
US8431390B2 (en) 2004-09-15 2013-04-30 Integenx Inc. Systems of sample processing having a macro-micro interface
US8454906B2 (en) 2007-07-24 2013-06-04 The Regents Of The University Of California Microfabricated droplet generator for single molecule/cell genetic analysis in engineered monodispersed emulsions
US8476063B2 (en) 2004-09-15 2013-07-02 Integenx Inc. Microfluidic devices
US8512538B2 (en) 2010-05-28 2013-08-20 Integenx Inc. Capillary electrophoresis device
US8557518B2 (en) 2007-02-05 2013-10-15 Integenx Inc. Microfluidic and nanofluidic devices, systems, and applications
US8584703B2 (en) 2009-12-01 2013-11-19 Integenx Inc. Device with diaphragm valve
US8672532B2 (en) 2008-12-31 2014-03-18 Integenx Inc. Microfluidic methods
US8748165B2 (en) 2008-01-22 2014-06-10 Integenx Inc. Methods for generating short tandem repeat (STR) profiles
US8763642B2 (en) 2010-08-20 2014-07-01 Integenx Inc. Microfluidic devices with mechanically-sealed diaphragm valves
US8841116B2 (en) 2006-10-25 2014-09-23 The Regents Of The University Of California Inline-injection microdevice and microfabricated integrated DNA analysis system using same
US9121058B2 (en) 2010-08-20 2015-09-01 Integenx Inc. Linear valve arrays
US9644623B2 (en) 2002-12-30 2017-05-09 The Regents Of The University Of California Fluid control structures in microfluidic devices
JP2019510504A (ja) * 2016-04-06 2019-04-18 グリーンライト バイオサイエンシーズ インコーポレーテッドGreenlight Biosciences,Inc. リボ核酸の無細胞的生産
US11274284B2 (en) 2015-03-30 2022-03-15 Greenlight Biosciences, Inc. Cell-free production of ribonucleic acid

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8685648B2 (en) * 2009-02-03 2014-04-01 Biohelix Corporation Endonuclease-enhanced helicase-dependent amplification
EP2611922A1 (en) 2010-08-31 2013-07-10 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways through protease manipulation
AU2014306074B2 (en) 2013-08-05 2018-08-30 Greenlight Biosciences, Inc. Engineered proteins with a protease cleavage site
EP3430154B1 (en) * 2016-03-14 2020-11-11 Rgene, Inc. Hyper-thermostable lysine-mutant ssdna/rna ligases
EP3565892A4 (en) 2017-01-06 2020-10-07 Greenlight Biosciences, Inc. Cell-free production of sugars
KR102894284B1 (ko) 2017-10-11 2025-12-03 그린라이트 바이오사이언시스, 아이엔씨. 뉴클레오시드 트리포스페이트 및 리보핵산 생산을 위한 방법 및 조성물
CN114990080B (zh) * 2021-07-01 2023-07-21 温州医科大学 一种赖氨酸突变的热稳定核酸连接酶

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US5374553A (en) * 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US5283173A (en) * 1990-01-24 1994-02-01 The Research Foundation Of State University Of New York System to detect protein-protein interactions
US5352778A (en) * 1990-04-26 1994-10-04 New England Biolabs, Inc. Recombinant thermostable DNA polymerase from archaebacteria
US5756334A (en) * 1990-04-26 1998-05-26 New England Biolabs, Inc. Thermostable DNA polymerase from 9°N-7 and methods for producing the same
CA2092317A1 (en) * 1990-09-28 1992-03-29 David H. Gelfand Purified thermostable nucleic acid polymerase enzyme from thermosipho africanus
AU664050B2 (en) * 1991-12-18 1995-11-02 Becton Dickinson & Company Process for lysing mycobacteria
CA2159081C (en) * 1993-03-24 2000-11-21 David Mead Dinucleotide restriction endonuclease preparations and methods of use
US6100078A (en) * 1994-04-01 2000-08-08 Gen-Probe Incorporated Purified DNA polymerase from bacillus stearothermophilus ATCC 12980
US6306588B1 (en) * 1997-02-07 2001-10-23 Invitrogen Corporation Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof
DE69836482T2 (de) * 1997-07-25 2007-09-27 Agene Research Institute, Co., Ltd., Kamakura Menschliches recq4 gen, das für eine helikase kodiert
US20010018192A1 (en) * 1998-02-12 2001-08-30 Terstappen Leon W.M.M. Labeled cells for use as an internal functional control in rare cell detection assays
EP1127135B1 (en) * 1998-10-30 2007-05-23 Cornell Research Foundation, Inc. High fidelity thermostable ligase and uses thereof
US6436677B1 (en) * 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
US6323009B1 (en) * 2000-06-28 2001-11-27 Molecular Staging, Inc. Multiply-primed amplification of nucleic acid sequences
EP1301205A1 (en) * 2000-07-12 2003-04-16 Invitrogen Corporation High fidelity polymerases and uses thereof

Cited By (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9651039B2 (en) 2002-12-30 2017-05-16 The Regents Of The University Of California Fluid control structures in microfluidic devices
US9644623B2 (en) 2002-12-30 2017-05-09 The Regents Of The University Of California Fluid control structures in microfluidic devices
US8420318B2 (en) 2004-06-01 2013-04-16 The Regents Of The University Of California Microfabricated integrated DNA analysis system
US9752185B2 (en) 2004-09-15 2017-09-05 Integenx Inc. Microfluidic devices
US8431390B2 (en) 2004-09-15 2013-04-30 Integenx Inc. Systems of sample processing having a macro-micro interface
US8431340B2 (en) 2004-09-15 2013-04-30 Integenx Inc. Methods for processing and analyzing nucleic acid samples
US8476063B2 (en) 2004-09-15 2013-07-02 Integenx Inc. Microfluidic devices
US8551714B2 (en) 2004-09-15 2013-10-08 Integenx Inc. Microfluidic devices
US8286665B2 (en) 2006-03-22 2012-10-16 The Regents Of The University Of California Multiplexed latching valves for microfluidic devices and processors
US8841116B2 (en) 2006-10-25 2014-09-23 The Regents Of The University Of California Inline-injection microdevice and microfabricated integrated DNA analysis system using same
US8557518B2 (en) 2007-02-05 2013-10-15 Integenx Inc. Microfluidic and nanofluidic devices, systems, and applications
US8454906B2 (en) 2007-07-24 2013-06-04 The Regents Of The University Of California Microfabricated droplet generator for single molecule/cell genetic analysis in engineered monodispersed emulsions
US8748165B2 (en) 2008-01-22 2014-06-10 Integenx Inc. Methods for generating short tandem repeat (STR) profiles
US8672532B2 (en) 2008-12-31 2014-03-18 Integenx Inc. Microfluidic methods
US8388908B2 (en) 2009-06-02 2013-03-05 Integenx Inc. Fluidic devices with diaphragm valves
US8584703B2 (en) 2009-12-01 2013-11-19 Integenx Inc. Device with diaphragm valve
US8512538B2 (en) 2010-05-28 2013-08-20 Integenx Inc. Capillary electrophoresis device
US8763642B2 (en) 2010-08-20 2014-07-01 Integenx Inc. Microfluidic devices with mechanically-sealed diaphragm valves
US9121058B2 (en) 2010-08-20 2015-09-01 Integenx Inc. Linear valve arrays
US9731266B2 (en) 2010-08-20 2017-08-15 Integenx Inc. Linear valve arrays
US11274284B2 (en) 2015-03-30 2022-03-15 Greenlight Biosciences, Inc. Cell-free production of ribonucleic acid
JP2019510504A (ja) * 2016-04-06 2019-04-18 グリーンライト バイオサイエンシーズ インコーポレーテッドGreenlight Biosciences,Inc. リボ核酸の無細胞的生産
JP7011599B2 (ja) 2016-04-06 2022-02-10 グリーンライト バイオサイエンシーズ インコーポレーテッド リボ核酸の無細胞的生産
JP2022062056A (ja) * 2016-04-06 2022-04-19 グリーンライト バイオサイエンシーズ インコーポレーテッド リボ核酸の無細胞的生産

Also Published As

Publication number Publication date
US20050064490A1 (en) 2005-03-24
WO2005030995A1 (en) 2005-04-07
US20060252083A1 (en) 2006-11-09
CN1856579A (zh) 2006-11-01
CA2535922A1 (en) 2005-04-07
EP1664347A1 (en) 2006-06-07
AU2004276790A1 (en) 2005-04-07

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