CA2535922A1 - Methods of synthesizing polynucleotides using thermostable enzymes - Google Patents
Methods of synthesizing polynucleotides using thermostable enzymes Download PDFInfo
- Publication number
- CA2535922A1 CA2535922A1 CA002535922A CA2535922A CA2535922A1 CA 2535922 A1 CA2535922 A1 CA 2535922A1 CA 002535922 A CA002535922 A CA 002535922A CA 2535922 A CA2535922 A CA 2535922A CA 2535922 A1 CA2535922 A1 CA 2535922A1
- Authority
- CA
- Canada
- Prior art keywords
- cell
- polynucleotide
- kit
- cloning vector
- reaction mixture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 108091033319 polynucleotide Proteins 0.000 title claims abstract description 77
- 239000002157 polynucleotide Substances 0.000 title claims abstract description 77
- 102000040430 polynucleotide Human genes 0.000 title claims abstract description 77
- 238000000034 method Methods 0.000 title claims abstract description 51
- 230000002194 synthesizing effect Effects 0.000 title claims abstract description 9
- 102000004190 Enzymes Human genes 0.000 title description 23
- 108090000790 Enzymes Proteins 0.000 title description 23
- 239000011541 reaction mixture Substances 0.000 claims abstract description 31
- 230000000295 complement effect Effects 0.000 claims abstract description 13
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- 241000588724 Escherichia coli Species 0.000 claims description 20
- 239000013599 cloning vector Substances 0.000 claims description 20
- 108091008146 restriction endonucleases Proteins 0.000 claims description 13
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 9
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 9
- 239000011535 reaction buffer Substances 0.000 claims description 8
- 239000005547 deoxyribonucleotide Substances 0.000 claims description 7
- 102000003844 DNA helicases Human genes 0.000 claims description 6
- 108090000133 DNA helicases Proteins 0.000 claims description 6
- 102100031780 Endonuclease Human genes 0.000 claims description 6
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims description 6
- 238000010367 cloning Methods 0.000 claims description 6
- 238000005382 thermal cycling Methods 0.000 claims description 6
- 108091028664 Ribonucleotide Proteins 0.000 claims description 4
- 125000002637 deoxyribonucleotide group Chemical group 0.000 claims description 4
- 239000005546 dideoxynucleotide Substances 0.000 claims description 4
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US50530003P | 2003-09-23 | 2003-09-23 | |
| US60/505,300 | 2003-09-23 | ||
| PCT/US2004/031267 WO2005030995A1 (en) | 2003-09-23 | 2004-09-23 | Methods of synthesizing polynucleotides using thermostable enzymes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2535922A1 true CA2535922A1 (en) | 2005-04-07 |
Family
ID=34393006
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002535922A Abandoned CA2535922A1 (en) | 2003-09-23 | 2004-09-23 | Methods of synthesizing polynucleotides using thermostable enzymes |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US20050064490A1 (https=) |
| EP (1) | EP1664347A1 (https=) |
| JP (1) | JP2007506430A (https=) |
| CN (1) | CN1856579A (https=) |
| AU (1) | AU2004276790A1 (https=) |
| CA (1) | CA2535922A1 (https=) |
| WO (1) | WO2005030995A1 (https=) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7445926B2 (en) | 2002-12-30 | 2008-11-04 | The Regents Of The University Of California | Fluid control structures in microfluidic devices |
| US7799553B2 (en) | 2004-06-01 | 2010-09-21 | The Regents Of The University Of California | Microfabricated integrated DNA analysis system |
| CN101073002B (zh) | 2004-09-15 | 2012-08-08 | 英特基因有限公司 | 微流体装置 |
| EP1979079A4 (en) | 2006-02-03 | 2012-11-28 | Integenx Inc | MICROFLUIDIC DEVICES |
| US7766033B2 (en) | 2006-03-22 | 2010-08-03 | The Regents Of The University Of California | Multiplexed latching valves for microfluidic devices and processors |
| US8841116B2 (en) | 2006-10-25 | 2014-09-23 | The Regents Of The University Of California | Inline-injection microdevice and microfabricated integrated DNA analysis system using same |
| US20110039303A1 (en) | 2007-02-05 | 2011-02-17 | Stevan Bogdan Jovanovich | Microfluidic and nanofluidic devices, systems, and applications |
| US8454906B2 (en) | 2007-07-24 | 2013-06-04 | The Regents Of The University Of California | Microfabricated droplet generator for single molecule/cell genetic analysis in engineered monodispersed emulsions |
| KR20110030415A (ko) | 2008-01-22 | 2011-03-23 | 인터젠엑스 인크. | 만능 샘플 제조 시스템 및 집적 분석 시스템에서의 용도 |
| KR20110111449A (ko) | 2008-12-31 | 2011-10-11 | 인터젠엑스 인크. | 미세유체 칩을 갖는 기구 |
| US8685648B2 (en) * | 2009-02-03 | 2014-04-01 | Biohelix Corporation | Endonuclease-enhanced helicase-dependent amplification |
| CN102459565A (zh) | 2009-06-02 | 2012-05-16 | 尹特根埃克斯有限公司 | 具有隔膜阀的流控设备 |
| US8584703B2 (en) | 2009-12-01 | 2013-11-19 | Integenx Inc. | Device with diaphragm valve |
| US8512538B2 (en) | 2010-05-28 | 2013-08-20 | Integenx Inc. | Capillary electrophoresis device |
| EP2606154B1 (en) | 2010-08-20 | 2019-09-25 | Integenx Inc. | Integrated analysis system |
| WO2012024657A1 (en) | 2010-08-20 | 2012-02-23 | IntegenX, Inc. | Microfluidic devices with mechanically-sealed diaphragm valves |
| EP2611922A1 (en) | 2010-08-31 | 2013-07-10 | Greenlight Biosciences, Inc. | Methods for control of flux in metabolic pathways through protease manipulation |
| AU2014306074B2 (en) | 2013-08-05 | 2018-08-30 | Greenlight Biosciences, Inc. | Engineered proteins with a protease cleavage site |
| BR112017020690A2 (pt) | 2015-03-30 | 2018-06-26 | Greenlight Biosciences Inc | produção livre de células de ácido ribonucleico |
| EP3430154B1 (en) * | 2016-03-14 | 2020-11-11 | Rgene, Inc. | Hyper-thermostable lysine-mutant ssdna/rna ligases |
| CA3020312A1 (en) * | 2016-04-06 | 2017-10-12 | Greenlight Biosciences, Inc. | Cell-free production of ribonucleic acid |
| EP3565892A4 (en) | 2017-01-06 | 2020-10-07 | Greenlight Biosciences, Inc. | Cell-free production of sugars |
| KR102894284B1 (ko) | 2017-10-11 | 2025-12-03 | 그린라이트 바이오사이언시스, 아이엔씨. | 뉴클레오시드 트리포스페이트 및 리보핵산 생산을 위한 방법 및 조성물 |
| CN114990080B (zh) * | 2021-07-01 | 2023-07-21 | 温州医科大学 | 一种赖氨酸突变的热稳定核酸连接酶 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5374553A (en) * | 1986-08-22 | 1994-12-20 | Hoffmann-La Roche Inc. | DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima |
| US5283173A (en) * | 1990-01-24 | 1994-02-01 | The Research Foundation Of State University Of New York | System to detect protein-protein interactions |
| US5352778A (en) * | 1990-04-26 | 1994-10-04 | New England Biolabs, Inc. | Recombinant thermostable DNA polymerase from archaebacteria |
| US5756334A (en) * | 1990-04-26 | 1998-05-26 | New England Biolabs, Inc. | Thermostable DNA polymerase from 9°N-7 and methods for producing the same |
| CA2092317A1 (en) * | 1990-09-28 | 1992-03-29 | David H. Gelfand | Purified thermostable nucleic acid polymerase enzyme from thermosipho africanus |
| AU664050B2 (en) * | 1991-12-18 | 1995-11-02 | Becton Dickinson & Company | Process for lysing mycobacteria |
| CA2159081C (en) * | 1993-03-24 | 2000-11-21 | David Mead | Dinucleotide restriction endonuclease preparations and methods of use |
| US6100078A (en) * | 1994-04-01 | 2000-08-08 | Gen-Probe Incorporated | Purified DNA polymerase from bacillus stearothermophilus ATCC 12980 |
| US6306588B1 (en) * | 1997-02-07 | 2001-10-23 | Invitrogen Corporation | Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof |
| DE69836482T2 (de) * | 1997-07-25 | 2007-09-27 | Agene Research Institute, Co., Ltd., Kamakura | Menschliches recq4 gen, das für eine helikase kodiert |
| US20010018192A1 (en) * | 1998-02-12 | 2001-08-30 | Terstappen Leon W.M.M. | Labeled cells for use as an internal functional control in rare cell detection assays |
| EP1127135B1 (en) * | 1998-10-30 | 2007-05-23 | Cornell Research Foundation, Inc. | High fidelity thermostable ligase and uses thereof |
| US6436677B1 (en) * | 2000-03-02 | 2002-08-20 | Promega Corporation | Method of reverse transcription |
| US6323009B1 (en) * | 2000-06-28 | 2001-11-27 | Molecular Staging, Inc. | Multiply-primed amplification of nucleic acid sequences |
| EP1301205A1 (en) * | 2000-07-12 | 2003-04-16 | Invitrogen Corporation | High fidelity polymerases and uses thereof |
-
2004
- 2004-09-23 WO PCT/US2004/031267 patent/WO2005030995A1/en not_active Ceased
- 2004-09-23 AU AU2004276790A patent/AU2004276790A1/en not_active Abandoned
- 2004-09-23 JP JP2006528170A patent/JP2007506430A/ja not_active Abandoned
- 2004-09-23 CN CNA2004800274720A patent/CN1856579A/zh active Pending
- 2004-09-23 CA CA002535922A patent/CA2535922A1/en not_active Abandoned
- 2004-09-23 EP EP04784918A patent/EP1664347A1/en not_active Withdrawn
- 2004-09-23 US US10/947,832 patent/US20050064490A1/en not_active Abandoned
-
2006
- 2006-06-26 US US11/426,422 patent/US20060252083A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20050064490A1 (en) | 2005-03-24 |
| WO2005030995A1 (en) | 2005-04-07 |
| US20060252083A1 (en) | 2006-11-09 |
| CN1856579A (zh) | 2006-11-01 |
| JP2007506430A (ja) | 2007-03-22 |
| EP1664347A1 (en) | 2006-06-07 |
| AU2004276790A1 (en) | 2005-04-07 |
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