CN1836677A - Substance and technology for producing substance capable of repairing pathogenic gene not using clone technology - Google Patents

Abstract

The present invention is non-cloning technology of producing gene repairing matter. By means of combination of Chinese medicine pharmacy and modern genetics, food-medicine fungi, fruit, vegetable and other material are treated through enzyme and fermenting engineering process including serial biochemical reactions to produce gene pharmaceutical and gene nutriological matter for repairing body's pathogenic genes. The gene pharmaceutical and gene nutriological matter of the present invention may be used as medicine and health article for tumor, hepatitis B, diabetes, gastrointestinal diseases, cardiac and cerebral vascular diseases, etc. and has no any toxic side effect.

Description

The material of technology for producing substance capable of repairing pathogenic gene not using clone and technology thereof

The present invention combines with modern genetics with the traditional Chinese medicine and pharmacy of China, relates to top new enzyme engineering of 21 century biological engineering front line science and field of fermentation engineering.Since its innovative point is the seventies in 20th century, the many opinions of world biotechnology authority have been broken through, and do not produce products such as human gene's medicine and pharmacology with stem cell through clone technology with them, but can reach the series of products of repairing some Disease-causing gene of human body with the same of the present invention's production, belong to and be used for the gene threpsology and the gene medicine and pharmacology material of gene therapy theory, and be active compound composition.The inventor of current Chinese biological engineering etc. from 1973 so far, have broken the so-called gene therapies of western developed country (seeing Appendix 1,2) such as USA and Europe day with the scientific and technological fact.

The present invention is that its inventor " making process for edible mushroom produced by using sugar acid ferment method " (sees Appendix 3, the patent No.: ZL88103922.5), " product of edible fungi and Chinese herbal medicine mixed fermentation brewing and method thereof " (see Appendix 4, the patent No.: ZL93104737.4), " edible and medicinal fungi and Chinese herbal medicine prepare gene medical product and new technique " (see Appendix 5: application number: 01109668.3) etc. system's application for a patent for invention.Now drawn the RMB of financing in 11 figure places, special project is used for the exploitation of native system patent of invention.

Feature of the present invention: be to select the functional edible medicinal of high-quality source for use on raw material, promptly select various edible, medicinal fungis and fruit tree and vegetable fruit, tuber, tuber, nontoxic wild fruit for use, or the like be raw material, do not add any chemosynthesis material and antiseptic, intend forming the compatibility of medicines in a prescription with approach or efficient functional by producing; And the aerobic rhizopus of selecting for use food industry now to use is a strain, break " tricarboxylic acid cycle " theory, not under aerobic condition, but anaerobic or dislike under the aseptic specific living condition such as oxygen, force it to use various enzymes, to the raw materials used series of chemical of carrying out, generate the compound substance composition that new gene medicine and pharmacology class and gene threpsology class etc. belong to the Chinese medicine and pharmacy theory, be prepared into serial medicine and health product that suitable efficient functional is arranged without any side effects.And anaerobic fermentation enzyme engineering theory has been proposed, set up " saccharic acid enzyme method technique ".The present invention's process fairly large commercial production of inventor and product, chemical examination detection, animal and clinical trial, relevant patient checking etc., facts have proved that its product can be used to repair the Disease-causing gene of difficult and complicated illness such as the tumor, hepatitis B, diabetes, cardiovascular and cerebrovascular disease, gastroenteropathy, acquired immune deficiency syndrome (AIDS), hypomnesis, slow down aging, sexual hypofunction of human body; It can be used for suppressing, blocking dna, RNAi being transcribed and duplicating variation, degenerating cell.This reaches health and happiness longevity to improving human life's quality, is indispensable medicine and health product, and compares world's gene therapy product super quality and competitive price several times now, and meaning is inestimable.

The present invention realizes that through following operation this Figure of description is seen in technological process.

First operation: select various high-quality edible and medicinal fungis for use, as Pleurotus ostreatus, Pleurotus sajor-caju, JINZHENGU, Lentinus Edodes, Tremella, Caulis Bambusae In Taeniam, Hericium erinaceus (Bull. Ex Fr.) Pers., Lasiosphaera Seu Calvatia, Rhizoma Gastrodiae, Poria, Polyporus, Ganderma lucidum, or the like, by the medical application of producing different product, promptly at different syndromes, the component content requirement of desired function curative effect material is in conjunction with country and enterprise product quality standard.According to the into contained various chemical analysis of raw material and water resource and air etc. of whenever buying goods wholesale, the testing result of mineral nitrogen (main refer to elements such as heavy metal and radioactivity) and micronutrient levels and noxious substance particularly, after removing the Toxic unfavorable factor, the prescription (see the 7th operation for example, the back together) of forming this batch product from host computer; Calculate by the aquatic foods product without exception when feeding intake, for example: with fresh Pleurotus ostreatus, Pleurotus sajor-caju, Deng, each is a ratio with 100 (as kilogram) Unit Weight for various raw materials, other are as Lentinus Edodes (avoiding with bright product), Tremella, Hericium erinaceus (Bull. Ex Fr.) Pers., Lasiosphaera Seu Calvatia, Rhizoma Gastrodiae, Poria, Ganderma lucidum etc., but but be also inequality (back with) of raw materials used equivalent, feed intake for 100 kilograms with 30 kilograms of ratios foldings in the high-quality dry product, weight (comprising volume) is calculated (1) by kilogram or kilogram or ton without exception.Also can only select the 1-3 kind, as producing nourishing health care liquid.30 kilograms of fresh product that are calculated to be 100 kilograms of bright product backs (Caulis Bambusae In Taeniam is calculated to be 100 kilograms with 10 kilograms) of its dry product, with its sum total metering that is converted to bright product, raw material and water ratio are 1: 4-15 calculates, and (or underground spring is better with the high-quality safe drinking water, the back is together) (2), the one, bright mushroom is cleaned; The 2nd, the mushroom dry product is earlier with 1: 1-2 health boiling water is contained and was soaked 1 hour, suctions moisture, cleans in immersion, its dip water is removed silt after, in the adding raw material pulverizing; Respectively bright mushroom and the mushroom dry product that suctions behind the moisture are all pulverized (3), again by the sum total metering that feeds intake, in the condensing reflux autoclave, prepare enough health boiling water (back is all proposes sterilization all with this understanding), and press the boiling water amount and add 0.2～0.3% Sal, raw material dropped in the boiling water stir, equilibrium liquid relaxing the bowels with purgatives of warm nature at 90～100 ℃, sterilize 1～2 hour (4), under the sealed, sterile condition, squeeze in the aseptic jar fermenter, jar, pond of aseptic fermenting cellar, its (digestion liquid) cooling (also can in autoclave) to 47 ℃, is carried out primary fermentation (5) after waiting to inoculate.

Second operation: autonomous production or choose food industry now surely with rhizopus strain (6), the patent No. by the inventor is again: 3 pages method in the ZL93104737.4 description (seeing Appendix 4), the enzyme juice alive (7) for preparing this rhizopus strain, calculate by the liquid measure to be fermented behind the digestion that each round is contained (kilogram etc.): 1. when liquid temperature drop to 45～32 ℃, the enzyme juice alive of access 3～5% earlier; 2. follow, calculate with this rhizopus dry strain 0.2～0.5%; Both each minutes, stirring and evenly mixing was treated in the fermentation liquid in 3 accesses.Simultaneously dry strain is pressed the liquid level estimation of different big or small rounds, suitably the allowance dry strain is inoculated and is spread on liquid level (all strains that connects, back are together) (8) at last, and leave standstill, add a cover, under 32～22 ℃ of conditions of liquid temperature, in sterilizing room, primary fermentation 24～48 hours (9).When fermentating liquid PH value from high to low, reduced to 4～3.0 o'clock, fungus mushrooms mash (10), prepare to enter the after fermentation (16) of the 3rd operation.

The 3rd operation: the every batch of production accounts for 60～70% or account for 30～40% by fungus mushroom mash weight, and fruit type mash weight accounts for 30～40% or account for 60～70% ratios (visible the 7th operation).By the first operation prescription combination principle, being calculated to be and selecting which fruit type for use is raw material, selects the fresh fruit of the nontoxic and noresidue cytotoxic drug of high-quality, for example: Fructus Mali pumilae, pears, mandarin orange, Fructus actinidiae chinensis, Fructus Lycopersici esculenti etc.; Wild fresh fruit category is as Fructus Pyracanthae (popular name), Fructus Rosae Normalis (popular name), Fructus Lycii, thorn confection (Fructus Rosae Laevigatae) etc. and tuber, tuber (Radix Dauci Sativae) etc.Also can only select 1-3 kind (as: producing common nutritional solution) as required, by each fruit that accounts for this batch of percentage calculation feed intake what the kilogram, add up to the total consumption of fruit type mash (11) by used various fruits again.In fruit feed intake total be 1 with the ratio of health water: 4-15; In autoclave (jar), prepare enough health boiling water (12), and press the Sal that the boiling water amount adds 0.2-0.3, throw in again through cleaning, roguing, fresh fruit raw material (13) after the pulverizing, under 85-95 ℃ of condition of equilibrium liquid temperature, the gas condensing reflux was sterilized 30-60 minute, reached aseptic (14), under aseptic condition, the jar fermenter of aseptic fermenting cellar is squeezed in sealing, jar, in the pond, by first, condition in two operations and method insert live enzyme juice and dry strain, ferment (15), after fermentation 2 hours, pH value was reduced to 5.0 o'clock, carried out after fermentation with mushroom mash mixing through primary fermentation again.How the mixed liquor percentage ratio of mushroom mash and fruit mash determines that what look its each batch production of plan earlier is the medicine of gene medicine and pharmacology; Or gene threpsology's health product; Still plain edition is decided (16) in order to replace carbonic acid and synthetic beverage or wine, beer beverage juice etc.It is mixed the back (dye assorted bacterium under aseptic condition as front or rear fermentation liquid, to sterilize, connect strain again), leave standstill again, add a cover, under 32-22 ℃ of condition of liquid temperature, carry out after fermentation (17), when the pH value of after fermentation liquid drops to 3.6, promptly stop fermentation (18).Or when (10) and (15), medicine and health substance beverage are produced in not mixed culture fermentation.

The 4th operation: after current after fermentation liquid mixes, the fermentation liquid pH value is 3.6 o'clock, carry out first time coarse filtration slagging-off (to slag exploitation in addition), blend for the first time, seasoning (19), necessarily squeeze in the condensing reflux autoclave (jar), to mixing mash at 85 ℃ of-95 ℃ of liquid relaxing the bowels with purgatives of warm nature, sterilize 1-2 hour to aseptic (20), squeeze in the aseptic sealed container, behind liquid temperature drop to 20 ℃, carry out the second time and filter (21), and carry out the precipitate exploitation.When chemical examination, after the quality inspection, seasoning for the second time, blend or be condensed into desired concn, reach quality standard (22) with each batch unanimity before and after the product, sealing and standing, cooling again, liquid temperature are in the time of-2 ℃, with filtrations (23) such as mocromembranes, sterilize through the instantaneously sterilising method, do not have this equipment, still use pasteurization 1-2 hour (24), when confirming not have assorted bacterium, during juice pH value 3.6-3.2, under aseptic condition, filling and package (25) becomes medicine and health product liquor series, comes into the market after the finished product quality inspection to sell (26).

The 5th operation: after the 4th operation enters (19), when blending, use edible ethanol, mash is contained amount of alcohol transfer to 16-38%, add the Mel (27) of 1-3%, enter the aging chamber, carry out 2 months to 24 months (28) of aging fermentation with osculum watt cylinder.Decision wine degree how much, decides on its product purpose.After 2 months, when assorted bacterium being arranged as detection, the aging supernatant is squeezed in the condensing reflux autoclave, 70 ℃-85 ℃ of liquid temperature, sterilize 1-2 hour (29), when liquid temperature drop to 15 ℃, chemically examine, quality inspection and blend for the third time, finely tune, make its identical product, every batch content of material all keeps consistent (30) up to standard with mouthfeel, fragrance etc., again-2 ℃ carry out mocromembrane filter with sterilization (31) after, enter the fill of the 4th operation, be packaged into medicine or health substance series beverage (25) and (26) of ethanol liquor, the deposit of aging is exploitation in addition.

The 6th operation: produce solid-state, special liquid form product.After (19) that enter the 4th operation, use the installations and facilities in medical industry modern times, concentrate (32), wherein gas utilizes through condensing reflux again.Its concentrated prerequisite: a kind of be the essential material composition of fermentation liquid by normal concentration content low should improve; Two kinds of sterile products that are based on the solid state of producing medicine series are produced (33) such as sheet (ball) agent, powder, electuaries behind pasteurization; Three kinds or the concentrated natural juice of work, aseptic other areas, the country of transporting to, select high-quality safe drinking water or spring water again for use, under aseptic condition, blend dilution, enter (25) and (26) of the 4th operation again for liquor medicine (oral liquid), health promoting beverage and nutritious drink or low drinks (34).

The 7th operation: on one to six operation basis, can increase by the 7th operation.Be exemplified below with prescription (11) according to prescription (1), (2): intend at diseases such as intestinal, stomach, liver, lung, order, brains, produce 10,000 kilograms of liquor medicine, mushroom accounts for 70%, and fruit accounts for 30%, promptly with bright product mushroom: Pleurotus ostreatus 100, Pleurotus sajor-caju 100, JINZHENGU 100; Dry product mushroom: Lentinus Edodes 60, Caulis Bambusae In Taeniam 30 (folding bright 300), Hericium erinaceus (Bull. Ex Fr.) Pers. 30, Rhizoma Gastrodiae 30, Tremella 30, Poria 30, Ganderma lucidum 30, Lasiosphaera Seu Calvatia 30; Folding is bright 1400 kilograms altogether, and water is 1: 5, is 7000 kilograms.Fresh fruit: Fructus Mali pumilae 100, Fructus actinidiae chinensis 100, Fructus Pyracanthae 100, Fructus Lycii 100, thorn confection 100, Radix Dauci Sativae 100, totally 600, with the water ratio be 1: 5, be 3000 kilograms; Amount to 10,000 kilograms.In order further to produce ethanol being arranged or not having ethanol two big series of medicine or nutrient and healthcare products and ordinary liquid beverage comprehensively and clearly; Product is divided into high, medium and low three class in purposes and price positioning.In aforementioned first, second, third operation, raw material and water ratio have been set forth.Producer gene medicine and pharmacology and gene threpsology class physical product, when the 4th operation (19), the medicated wine (35) or the gene curative effect that contain the effective in cure function of ethanol 16-18% through aging (28) increase production are big, its raw material is little with the ratio of fermentation water, as being 1: 4-8, the material component content is higher, increases to produce the pharmaceutical oral liquid (36) that contains ethanol 1～3%; Produce nourishing beverage, increase: 1. the range of the particularly important composition kind of material composition is complete, and content is higher, produces the medicine or the health care kind (37) that contain ethanol 4-15%; 2. produce ethanol content 38%～8% serial nutritious wine (containing high-grade valency) product (38); 3. when the 6th operation (32), after carrying ethanol and concentrating, dilution is not for there being or having the common nourishing beverage of ethanol (39) again; 4. when the 3rd operation (18), through the 4th process flow, directly produce full fermentation preservative free, additive-free and no alcoholic acid low nutritious drink (40), 5. when first process flow (1), (2), only with the mushroom raw material or only select the 1-3 kind for use, as Pleurotus ostreatus, Pleurotus sajor-caju, Lentinus Edodes etc.; Or when the 3rd process flow (11), only with fruit type or only select the 1-3 kind for use, as Fructus Mali pumilae, Fructus Lycopersici esculenti, Fructus Lycii etc., mushroom and the not mixed culture fermentation of fruit liquid, or not mixed culture fermentation when operation (10) or (15), produce medicine or health product, or there is or do not have the full fermented beverage of alcoholic acid plain edition (41) with the rarest light production of nutrition in this operation, 6. or at process flow (1), (2) during with (11), only use mushroom 1-3 kind and fruit 1-3 kind, full mixed culture fermentation production plain edition beverage (42).

The 7th operation is that the characteristic innovation divides high, medium and low with the color on price grade.High-grade valency is to contain ethanol 19-20% to produce world-class high-grade valency mushroom fruit wine class; Middle-grade valency is medicine and the health product that are used for gene therapy, the price appropriateness, and the medicinal substances component content is up to standard quite high; Low-grade valency is the common auxotype beverage that price is low, output is big.

More than process order in the 7th operation, all to directly enter (22), (23), (24), (25), (26) of the 4th operation respectively.

The gene medicine and pharmacology class that the present invention produces and the physical product (not comprising common nourishing beverage) of gene threpsology class, the condition that it is necessary, its liquor or solid-state nutrition and the pharmaceutical substance that all contains more than 100 kind of effective active have unique sweet, sour, comprehensive local flavors such as mushroom is fragrant, fruital.Chemically examine repeatedly, detect through us, clinical trial, consumer uses the back and confirms: so it can repair some Disease-causing gene of human body, be rich in 18 kinds with upper amino acid comprising it, contained multiple platform polysaccharide of 3% above mushroom and interferon, nucleic acid, polypeptide, various active enzyme or the like; Contain about 50 kinds active organic minerals and trace element such as phosphorus to the human body beneficial, ferrum, calcium, manganese, zinc, strontium, selenium, molybdenum, bismuth; Contain multivitamin; Fat-free, low in calories, or the like, to human body (comprising excessive drinking) without any side effects.According to Chinese medicine and pharmacy and gene threpsology's theory, make human body cause illness's gene or equipotential Disease-causing gene, to the medicine and the material of nutrition, abundant choice is arranged, so all be the new and high technology and the product that can be adapted to promote the well-being of mankind from depth and broadness.Only stand-by gene mapping equipment etc. is managed enterprising step card from base now in theory, but is not the scope of the invention.

(2) adnexa one to adnexa five in after.

Claims (5)

1, the material of technology for producing substance capable of repairing pathogenic gene not using clone and technology thereof is characterized in that: select for use food industry to make strain with aerobic rhizopus, raw material is fermented producer gene medicine and threpsology's series products under anaerobic or suspicion oxygen condition.On raw material, select for use on the one hand various edible, medicinal fungis 1 to some kinds, be process flow (1), (2), two aspects select for use fruit tree and vegetable fruit and stem (piece), root (piece), nontoxic wild fruit or the like fruit 1～some kinds (11) to be raw material, and its raw material and water ratio are 1: 4-15, be equipped with health boiling water (2), add 0.2-0.3% Sal, raw material pulverizing (3) is under the condensing reflux condition, after being cooled to 47 ℃ with 90-100 ℃ of liquid temperature sterilization (4), treat inoculation fermentation (5).Select food industry to do kind of a bacterium (6) with rhizopus, the enzyme juice alive (7) of preparation strain, be cooled to 45 ℃-32 ℃ when being equipped with fermentation liquid, divide the rhizopus dry strain (8) that inserts 3-5% juice alive and 0.2-0.5% for 3 times, under 32-22 ℃ of condition of liquid temperature, leave standstill, add a cover, ferment 24-48 hour (9), when fermentating liquid PH value reduces to 3.6, get mushroom class mash (10), after fermentation to be changed over to (16).

2, according to the method for claim 1, select for use fruit type 1～some kinds to make raw material, it is characterized in that: account for 60-70% by the mushroom mash, or accounting for 40-30%, the fruit type mash accounts for 40-30%, or accounts for 60-70%, calculate fruit raw material throwing amount always (11), the fresh fruit product are 1 with the water ratio: 4-15, prepare enough health boiling water (12), and add the Sal of 0.2-0.3; Fruit is cleaned, is pulverized and drop into boiling water (13), 30-60 minute (14) of sterilizing in 85-95 ℃ of liquid temperature.Method and condition according to operation (6), (7), (8), (9) inoculated, fermentation (15).When the fruit fermentating liquid PH value reduces to 5.0, again with primary fermentation liquid mixed culture fermentation (16), after fermentation (17), when pH value reduces to 3.6, promptly stop fermentation (18), or when operation (10) or (15), not mixed culture fermentation all reaches and produces medicine and health product and series beverage.

3, according to claim 1,2 method, it is characterized in that: carry out filter cleaner (19) blending and tasting for the first time, mix mash in 1-2 hour (20) of 85-95 ℃ of sterilization, behind the liquid temperature drop to 20 ℃, filter for the second time (21), for the second time blending and tasting (22) up to standard, in the time of-2 ℃, filter (23) with mocromembrane etc., through instantaneously sterilising or pasteurization (24), mash pH value 3.6-3.2, fill (25).

4, according to claim 1,2,3, it is characterized in that after operation enters (19), the one: use edible ethanol when blending, mash is contained ethanol transfer to 16-38% (27), use osculum watt cylinder, again 2 months-24 months (28) of aging fermentation, with 1-2 hour (29) of 70-85 ℃ of sterilization, through operation blending and tasting (30) up to standard, filter (31) and make nutrition drinks series again, enter (25).Production contains ethanol 16-18% medicated wine (35) or produces the having of 0-3%, no ethanol oral liquid (36) or produce 4-15% ethanol beverage (37) production and contains ethanol 38-8% drinks (38).So feature of the present invention is also being produced complete ferment, do not add chemosynthesis material, preservative free, non-additive medicine, health product and low nutritious drink.After carrying ethanol and concentrating, dilution is ferment entirely no ethanol and a low ethanol beverage (40) of raw material production low concentration nutrition for being and not being ethanol beverage (39) or with 1-3 kind mushroom, fruit again; On operation (19) basis, concentrate to produce solid pharmaceutical (33) such as sheet (ball) agent, powder, electuary or make to concentrate natural juice and transport other area, country to, produce medicine, health promoting beverage and nutritious drink or at low drinks below 38% (34).

5, according to claim 1,2,3,4 method, be characterised in that the prescription when process flow (1) (2) or (11), or only make raw material with edible and medicinal fungi, perhaps only make raw material with " fruit " class, medicine and health product and beverage are produced in not mixed culture fermentation (41) or mushroom and fruit mixed culture fermentation (42).

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