CN105106454A - Natural biological preparation for improving irritable premature climacteric syndromes and method for preparing natural biological preparation - Google Patents
Natural biological preparation for improving irritable premature climacteric syndromes and method for preparing natural biological preparation Download PDFInfo
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Abstract
The invention provides a method for preparing a natural biological preparation for improving irritable premature climacteric syndromes. The method includes fermenting ground mixtures of mixed plants by the aid of mixed microorganisms to obtain the natural biological preparation. The mixed plants include, by weight, 10-20 parts of radix puerariae, 10-20 parts of sea buckthorn and 50-80 parts of grape seeds. The mixed microorganisms comprise, by weight, 1-2 parts of yeast and 15-25 parts of lactic acid bacteria. The invention further provides the natural biological preparation prepared by the aid of the method. The natural biological preparation and the method have the advantages that the natural biological preparation has a function of improving the irritable premature climacteric syndromes, effective components of the plants can be converted by the aid of microorganism mixed fermentation techniques, accordingly, the natural biological preparation can be easily absorbed and utilized, obvious effects can be realized, the irritable premature climacteric syndromes can be ecologically remedied by the natural biological preparation, and the like.
Description
Technical field
The present invention relates to a kind of biological preparation, particularly relating to a kind of for improving irritability climacteric natural biological preparation shifting to an earlier date syndrome and preparation method thereof.
Background technology
Within 40 years old, later women can step into an important stage---climacteric in life, and this is also the transition period that woman moves towards old and feeble.Medically, the appearance of Women’s climacteric is generally the age at 45 years old to 55 years old, in the last few years, the transformation of the role born socially due to women and the overdraw of health hyperenergia and do not take good care of in time, climacteric often shifts to an earlier date, and some woman even just there will be involutional symptom when one's late 30s, wherein, mainly with Hypomenorrhea even amenorrhea for cardinal symptom, now, amenorrhea that women is too early and climacteric can be avoided in advance if nursed one's health timely.At present, the reason that Women’s climacteric shifts to an earlier date is owing to finding that ovarian failure is not but paid attention to greatly, and then causes too early amenorrhea; Also have plenty of because life stress is large, cause psychological unbalance; What is more is the senilism eat of diet products improperly in order to lose weight and cause ovarian function.
Doctor trained in Western medicine thinks that climacteric is that ovarian failure causes in advance, and treatment should be started with from complementing estrogen, and this method seems and takes effect very fast, but basic reason does not obtain medical treatment, so cure the symptoms, not the disease.And controversies in hormone replacement in the elderly, also usually can cause the side effect such as metrorrhagia, leucorrhoea grow in quantity.
By the Research statistics of female patient shifted to an earlier date climacteric, find that most of patient is the working woman that knowledge hierarchy is high, living condition is good.These women because operating pressure is large, rhythm of life is fast and affect endocrine, occurs the symptom that some climacteric shift to an earlier date, as baffled irritated, insomnia, menoxenia etc.Also there are some researches show: radical pair saccharide, lipid, protein, enzyme, nucleic acid all have infringement, and free radical is the toxic product of the infringement self constantly produced in organism metabolism process, can participate in the pathological processes of body.Climacteric women free-radical contents raises, and the activity decrease of free radical scavenging enzyme, thus cause free radical metabolism disorderly.Free-radical contents rising can cause Cardiovascular Damage widely, causes irritability climacteric to shift to an earlier date the generation of syndrome.And take antioxidant and can alleviate oxidativestress damage, effectively can improve irritability climacteric shifts to an earlier date syndrome.
The technical scheme of existing patent of invention disclosed in CN103652872A, CN104115962A, CN103566347A, all develop with multiple Chinese herbal medicine or its extract, Chinese herbal medicine needs through concocting or processing, and after effective component extracting, there is remarkable result the side of taking; If directly taken without processing, then the obstacle owing to absorbing, effective ingredient not easily utilizes, DeGrain.
Summary of the invention
For above-mentioned drawback, the invention provides a kind of natural biological preparation shifting to an earlier date syndrome for improving irritability climacteric, is for raw material is obtained by compound microorganism ferments with the crushed mixture of composite plant.The present invention adopts country to defend strain in " can be used for the strain list of food " of planning commission's bulletin, adopts conventional plant and fruit to be raw material, carries out the biological preparation of composite fermentation.The present invention combines the effective ingredient of the first kind in existing health product and Equations of The Second Kind, the active component of a large amount of oxidation resistance is rich in product, such as: flavonoid, soaping agents, aminoacid, glutathion, vitamin C, vitamin E, SOD, lactate and various trace elements etc.
Further, present inventor surprisingly finds, when containing a large amount of Semen Vitis viniferae in composite plant, in the natural biological preparation obtained, the content that the material of non-oxidizability specifically comprises linoleic acid, multiple polyphenol, procyanidin etc. can increase greatly, in addition, and the vitamin B1 also containing high-load, these materials are for women, and especially climacteric crowd is especially favourable.
Under above-mentioned background and achievement in research, technical scheme of the present invention provides a kind of preparation method shifting to an earlier date the natural biological preparation of syndrome for improving irritability climacteric, it is characterized in that, a kind ofly obtain the preparation method of described natural biological preparation with the crushed mixture of composite plant for raw material is undertaken fermenting by complex microorganism, wherein:
The component of described composite plant and weight proportion thereof are 10-20 part Radix Puerariae, 10-20 part Fructus Hippophae and 50-80 part Semen Vitis viniferae;
The component of described complex microorganism and weight proportion thereof are 1-2 part yeast and 15-25 part lactobacillus, and wherein, described yeast is saccharomyces cerevisiae (Saccharomycescerevisiae, ACCC20065); Described lactobacillus is bacillus acidophilus (Lactobacilusacidophilus, ACCC10637), Lactobacillus plantarum (Lactobacilusplantarum, ACCC11118), the mixing of bacillus bifidus (Bifidobacterium, ACCC11054).
In a preferred embodiment of the invention, the weight proportion of bacillus acidophilus, Lactobacillus plantarum and bacillus bifidus is 2-3:2-3:5-10.
In another embodiment, described preparation method is specially:
Step 1: ground by described composite plant, with brown sugar and water mix and blend, obtains the pastel that ferments;
Step 2: add saccharomyces cerevisiae liquid in described fermentation pastel, sealed fermenting 5-8 days at the temperature of 25-30 DEG C;
Step 3: the mixed bacteria liquid then adding bacillus acidophilus, Lactobacillus plantarum and bacillus bifidus, sealed fermenting 10-15 days, obtains fermented product at the temperature of 30-37 DEG C.
Further, described preparation method also comprises the preparation of bacterium liquid, comprising: in the mixed-culture medium of brown sugar and water at the temperature of 25-30 DEG C airtight cultivation saccharomyces cerevisiae 10-15 days, obtain described saccharomyces cerevisiae liquid; In the mixed-culture medium of brown sugar and water at the temperature of 30-37 DEG C the mixed bacteria 10-15 days of airtight cultivation bacillus acidophilus, Lactobacillus plantarum and bacillus bifidus, obtain described mixed bacteria liquid.
Further, described preparation method also comprises carries out drip washing, concentrated, sterilization treatment successively to described fermented product, finally carries out the preparation of dosage form.
In a preferred embodiment of the invention, described preparation method is:
Step 1: brown sugar and water are mixed to get mixed-culture medium with the weight proportion of 5-10:90-98, be divided into two parts, a copy of it is airtight cultivation saccharomyces cerevisiae 10-15 days at the temperature of 25-30 DEG C, obtain described saccharomyces cerevisiae liquid, the mixed bacteria 10-15 days of another part airtight cultivation bacillus acidophilus, Lactobacillus plantarum and bacillus bifidus at the temperature of 30-37 DEG C, obtain described mixed bacteria liquid, wherein, the weight proportion of strain and mixed-culture medium is 1:80-120;
Step 2: described composite plant is ground at least 30-50 object granule, with brown sugar and water mix and blend, obtain the pastel that ferments, wherein, the weight proportion of composite plant, brown sugar, pure water is 35-60:2-5:40-60;
Step 3: add saccharomyces cerevisiae liquid in described fermentation pastel, sealed fermenting 5-8 days at the temperature of 25-30 DEG C, wherein, saccharomyces cerevisiae liquid is 1:15-20 with the weight proportion of fermentation pastel;
Step 4: the mixed bacteria liquid then adding bacillus acidophilus, Lactobacillus plantarum and bacillus bifidus, at the temperature of 30-37 DEG C, sealed fermenting 10-15 days, obtains fermented product, and wherein, mixed bacteria liquid is 1:15-20 with the weight proportion of fermentation pastel;
Step 5: add water drip washing again after soaking in described fermented product, then carries out the 20-30% being concentrated into original volume at 40-50 DEG C of temperature by leacheate; Subsequently concentrated solution is carried out sterilizing at 60-70 DEG C of temperature, sterilization time is 10-30 minute, is finally preserved at 2-4 DEG C of temperature by sterilized solution;
Step 6: preparation dosage form, obtains described natural biological preparation.
Take for convenience, preferably, further, described dosage form comprises solid formulation, oral liquid in the present invention, wherein:
By sterilized solution, sugar, protective agent with the weight proportion of 15-30:60-90:3-5, join successively in granulator, obtain described solid formulation, wherein, the flow of described granulator is 10-20ml/ minute, and inlet temperature is 60-80 DEG C, leaving air temp is 40-60 DEG C, and drop temperature is 25-35 DEG C;
Sterilized solution, sugar, protective agent are weighed with the weight proportion of 45-50:45-50:5-10, first dissolving adding suitable quantity of water in sugar and protective agent, adding sterilized solution after cooling, after stirring clarification, obtaining described oral liquid.
Wherein, described sugar comprises any one or a few in glucose, sucrose, fructose, lactose, erythritol, threitol, xylitol; Described protective agent comprise in maltodextrin, corn starch, sucralose, citric acid any one or a few.
In one particular embodiment of the present invention, described preparation method comprises the following steps:
The first step, production strain
By the saccharomyces cerevisiae (Saccharomycescerevisiae bought from Chinese agriculture Microbiological Culture Collection administrative center, ACCC20065) transfer in the conical flask of Rhizoma Solani tuber osi fluid medium, at 25-30 DEG C, cultivate 48-72 hour under 180-220rpm oscillating condition, take out after culture medium muddiness; Bacillus acidophilus (Lactobacillusacidophilus, ACCC10637), Lactobacillus plantarum (Lactobacillusplantarum, ACCC11118), bacillus bifidus (Bifidobacterium, ACCC11054), transfer in the conical flask of MRS fluid medium respectively, under 35-39 DEG C of condition, cultivate 48-72 hour, take out after culture medium muddiness, then put into 2-4 DEG C of Refrigerator store stand-by;
The preparation of second step, bacterium liquid
Brown sugar and water are mixed to get mixed-culture medium with the weight proportion of 5-10:90-98, be divided into two parts, a copy of it is airtight cultivation saccharomyces cerevisiae 10-15 days at the temperature of 25-30 DEG C, obtain described saccharomyces cerevisiae liquid, the mixed bacteria 10-15 days of another part airtight cultivation bacillus acidophilus, Lactobacillus plantarum and bacillus bifidus at the temperature of 30-37 DEG C, obtain described mixed bacteria liquid, wherein, the weight proportion of strain and mixed-culture medium is 1:80-120;
The preparation of the 3rd step, fermented product
(1) described composite plant is ground at least 30-50 object granule, with brown sugar and water mix and blend, obtain the pastel that ferments, wherein, the weight proportion of composite plant, brown sugar, pure water is 35-60:2-5:40-60;
(2) in described fermentation pastel, add saccharomyces cerevisiae liquid, sealed fermenting 5-8 days at the temperature of 25-30 DEG C, wherein, saccharomyces cerevisiae liquid is 1:15-20 with the weight proportion of fermentation pastel;
(3) then add the mixed bacteria liquid of bacillus acidophilus, Lactobacillus plantarum and bacillus bifidus, at the temperature of 30-37 DEG C, sealed fermenting 10-15 days, obtains fermented product, and wherein, mixed bacteria liquid is 1:15-20 with the weight proportion of fermentation pastel;
Prepared by the 4th step, concentrated solution
(1) add water in fermentation tank, make solid-to-liquid ratio reach 1:1, soak 1 day, then drip washing;
(2) leacheate carries out at 40-50 DEG C the 20-30% being concentrated into original volume;
(3) subsequently concentrated solution is carried out sterilizing at 60-70 DEG C of temperature, sterilization time is 10-30 minute; Finally sterilized solution is preserved at 2-4 DEG C of temperature, stand-by;
The preparation of the 5th step, dosage form
(1) solid formulation preparation
Material is taken by following weight proportion: sterilized solution liquid 15-30, erythritol 15-30, lactose 30-60, protective agent 3-5; First erythritol and lactose are added spraying dry one-step-granulating method successively, then start to spray into sterilized solution, flow 10-20ml/ minute, finally spray into the protective agent be dissolved in suitable quantity of water, the inlet temperature 60-80 DEG C of granulator, leaving air temp 40-60 DEG C, discharging when temperature drops to 25-35 DEG C in day with fog to be painted, obtains solid formulation;
(2) oral liquid preparation
Material is taken by following weight proportion: sterilized solution 45-50, erythritol 45-50, protective agent 5-10; First erythritol and protective agent are added appropriate hot distilled water stirring and dissolving, after being cooled to 25-35 DEG C, add sterilized solution, stir after solution is clarified, obtain oral liquid.
Technical scheme of the present invention also comprises any one preparation method above-mentioned and prepares natural biological preparation, shifts to an earlier date syndrome for improving irritability climacteric.
The present invention adopts country to defend strain in " can be used for the strain list of food " of planning commission's bulletin, adopts conventional plant and fruit to be raw material, carries out the natural biological preparation of composite fermentation.Advantage of the present invention is: possess and improve the function that irritability climacteric shifts to an earlier date syndrome, transformed by the effective ingredient of microorganism composite fermentation technology to plant, make it have and more easily absorb, the features such as Be very effective a kind ofly shift to an earlier date to irritability climacteric the natural biological preparation that syndrome carries out restoration of the ecosystem.
Detailed description of the invention
The invention provides a kind of preparation method shifting to an earlier date the natural biological preparation of syndrome for improving irritability climacteric, it is characterized in that, a kind ofly obtain the preparation method of described natural biological preparation with the crushed mixture of composite plant for raw material is undertaken fermenting by complex microorganism, wherein:
The component of described composite plant and weight proportion thereof are 10-20 part Radix Puerariae, 10-20 part Fructus Hippophae and 50-80 part Semen Vitis viniferae;
The component of described complex microorganism and weight proportion thereof are 1-2 part yeast and 15-25 part lactobacillus, and wherein, described yeast is saccharomyces cerevisiae (Saccharomycescerevisiae, ACCC20065); Described lactobacillus is bacillus acidophilus (Lactobacilusacidophilus, ACCC10637), Lactobacillus plantarum (Lactobacilusplantarum, ACCC11118), the mixing of bacillus bifidus (Bifidobacterium, ACCC11054).
In a preferred embodiment of the invention, the weight proportion of bacillus acidophilus, Lactobacillus plantarum and bacillus bifidus is 2-3:2-3:5-10.
In another embodiment, described preparation method is specially:
Step 1: ground by described composite plant, with brown sugar and water mix and blend, obtains the pastel that ferments;
Step 2: add saccharomyces cerevisiae liquid in described fermentation pastel, sealed fermenting 5-8 days at the temperature of 25-30 DEG C;
Step 3: the mixed bacteria liquid then adding bacillus acidophilus, Lactobacillus plantarum and bacillus bifidus, sealed fermenting 10-15 days, obtains fermented product at the temperature of 30-37 DEG C.
Further, described preparation method also comprises the preparation of bacterium liquid, comprising: in the mixed-culture medium of brown sugar and water at the temperature of 25-30 DEG C airtight cultivation saccharomyces cerevisiae 10-15 days, obtain described saccharomyces cerevisiae liquid; In the mixed-culture medium of brown sugar and water at the temperature of 30-37 DEG C the mixed bacteria 10-15 days of airtight cultivation bacillus acidophilus, Lactobacillus plantarum and bacillus bifidus, obtain described mixed bacteria liquid.
Further, described preparation method also comprises carries out drip washing, concentrated, sterilization treatment successively to described fermented product, finally carries out the preparation of dosage form.
In a preferred embodiment of the invention, described preparation method is:
Step 1: brown sugar and water are mixed to get mixed-culture medium with the weight proportion of 5-10:90-98, be divided into two parts, a copy of it is airtight cultivation saccharomyces cerevisiae 10-15 days at the temperature of 25-30 DEG C, obtain described saccharomyces cerevisiae liquid, the mixed bacteria 10-15 days of another part airtight cultivation bacillus acidophilus, Lactobacillus plantarum and bacillus bifidus at the temperature of 30-37 DEG C, obtain described mixed bacteria liquid, wherein, the weight proportion of strain and mixed-culture medium is 1:80-120;
Step 2: described composite plant is ground at least 30-50 object granule, with brown sugar and water mix and blend, obtain the pastel that ferments, wherein, the weight proportion of composite plant, brown sugar, pure water is 35-60:2-5:40-60;
Step 3: add saccharomyces cerevisiae liquid in described fermentation pastel, sealed fermenting 5-8 days at the temperature of 25-30 DEG C, wherein, saccharomyces cerevisiae liquid is 1:15-20 with the weight proportion of fermentation pastel;
Step 4: the mixed bacteria liquid then adding bacillus acidophilus, Lactobacillus plantarum and bacillus bifidus, at the temperature of 30-37 DEG C, sealed fermenting 10-15 days, obtains fermented product, and wherein, mixed bacteria liquid is 1:15-20 with the weight proportion of fermentation pastel;
Step 5: add water drip washing again after soaking in described fermented product, then carries out the 20-30% being concentrated into original volume at 40-50 DEG C of temperature by leacheate; Subsequently concentrated solution is carried out sterilizing at 60-70 DEG C of temperature, sterilization time is 10-30 minute, is finally preserved at 2-4 DEG C of temperature by sterilized solution;
Step 6: preparation dosage form, obtains described natural biological preparation.
Take for convenience, preferably, further, described dosage form comprises solid formulation, oral liquid in the present invention, wherein:
By sterilized solution, sugar, protective agent with the weight proportion of 15-30:60-90:3-5, join successively in granulator, obtain described solid formulation, wherein, the flow of described granulator is 10-20ml/ minute, and inlet temperature is 60-80 DEG C, leaving air temp is 40-60 DEG C, and drop temperature is 25-35 DEG C;
Sterilized solution, sugar, protective agent are weighed with the weight proportion of 45-50:45-50:5-10, first dissolving adding suitable quantity of water in sugar and protective agent, adding sterilized solution after cooling, after stirring clarification, obtaining described oral liquid.
Wherein, described sugar comprises any one or a few in glucose, sucrose, fructose, lactose, erythritol, threitol, xylitol; Described protective agent comprise in maltodextrin, corn starch, sucralose, citric acid any one or a few.
In one particular embodiment of the present invention, described preparation method comprises the following steps:
The first step, production strain
By the saccharomyces cerevisiae (Saccharomycescerevisiae bought from Chinese agriculture Microbiological Culture Collection administrative center, ACCC20065) transfer in the conical flask of Rhizoma Solani tuber osi fluid medium, at 25-30 DEG C, cultivate 48-72 hour under 180-220rpm oscillating condition, take out after culture medium muddiness; Bacillus acidophilus (Lactobacillusacidophilus, ACCC10637), Lactobacillus plantarum (Lactobacillusplantarum, ACCC11118), bacillus bifidus (Bifidobacterium, ACCC11054), transfer in the conical flask of MRS fluid medium respectively, under 35-39 DEG C of condition, cultivate 48-72 hour, take out after culture medium muddiness, then put into 2-4 DEG C of Refrigerator store stand-by;
The preparation of second step, bacterium liquid
Brown sugar and water are mixed to get mixed-culture medium with the weight proportion of 5-10:90-98, be divided into two parts, a copy of it is airtight cultivation saccharomyces cerevisiae 10-15 days at the temperature of 25-30 DEG C, obtain described saccharomyces cerevisiae liquid, the mixed bacteria 10-15 days of another part airtight cultivation bacillus acidophilus, Lactobacillus plantarum and bacillus bifidus at the temperature of 30-37 DEG C, obtain described mixed bacteria liquid, wherein, the weight proportion of strain and mixed-culture medium is 1:80-120;
The preparation of the 3rd step, fermented product
(1) described composite plant is ground at least 30-50 object granule, with brown sugar and water mix and blend, obtain the pastel that ferments, wherein, the weight proportion of composite plant, brown sugar, pure water is 35-60:2-5:40-60;
(2) in described fermentation pastel, add saccharomyces cerevisiae liquid, sealed fermenting 5-8 days at the temperature of 25-30 DEG C, wherein, saccharomyces cerevisiae liquid is 1:15-20 with the weight proportion of fermentation pastel;
(3) then add the mixed bacteria liquid of bacillus acidophilus, Lactobacillus plantarum and bacillus bifidus, at the temperature of 30-37 DEG C, sealed fermenting 10-15 days, obtains fermented product, and wherein, mixed bacteria liquid is 1:15-20 with the weight proportion of fermentation pastel;
Prepared by the 4th step, concentrated solution
(1) add water in fermentation tank, make solid-to-liquid ratio reach 1:1, soak 1 day, then drip washing;
(2) leacheate carries out at 40-50 DEG C the 20-30% being concentrated into original volume;
(3) subsequently concentrated solution is carried out sterilizing at 60-70 DEG C of temperature, sterilization time is 10-30 minute; Finally sterilized solution is preserved at 2-4 DEG C of temperature, stand-by;
The preparation of the 5th step, dosage form
(1) solid formulation preparation
Material is taken by following weight proportion: sterilized solution liquid 15-30, erythritol 15-30, lactose 30-60, protective agent 3-5; First erythritol and lactose are added spraying dry one-step-granulating method successively, then start to spray into sterilized solution, flow 10-20ml/ minute, finally spray into the protective agent be dissolved in suitable quantity of water, the inlet temperature 60-80 DEG C of granulator, leaving air temp 40-60 DEG C, discharging when temperature drops to 25-35 DEG C in day with fog to be painted, obtains solid formulation;
(2) oral liquid preparation
Material is taken by following weight proportion: sterilized solution 45-50, erythritol 45-50, protective agent 5-10; First erythritol and protective agent are added appropriate hot distilled water stirring and dissolving, after being cooled to 25-35 DEG C, add sterilized solution, stir after solution is clarified, obtain oral liquid.
Present invention also offers any one preparation method described above and prepare natural biological preparation, shifting to an earlier date syndrome for improving irritability climacteric.
Below by following examples and application examples technical scheme of the present invention explained further and illustrate.
Embodiment 1
A kind of preparation method of solid formulation of natural biological preparation:
The first step, production strain
By the saccharomyces cerevisiae (Saccharomycescerevisiae bought from Chinese agriculture Microbiological Culture Collection administrative center, ACCC20065) transfer in the conical flask of Rhizoma Solani tuber osi fluid medium, at 25-30 DEG C, cultivate 48-72 hour under 180-220rpm oscillating condition, take out after culture medium muddiness; Bacillus acidophilus (Lactobacillusacidophilus, ACCC10637), Lactobacillus plantarum (Lactobacillusplantarum, ACCC11118), bacillus bifidus (Bifidobacterium, ACCC11054), transfer in the conical flask of MRS fluid medium respectively, under 35-39 DEG C of condition, cultivate 48-72 hour, take out after culture medium muddiness, then put into 2-4 DEG C of Refrigerator store stand-by;
The preparation of second step, bacterium liquid
Brown sugar and water are mixed to get mixed-culture medium with the weight proportion of 5:95, be divided into two parts, a copy of it airtight cultivation saccharomyces cerevisiae 10 days at the temperature of 28 DEG C, obtain described saccharomyces cerevisiae liquid, the mixed bacteria of another part airtight cultivation bacillus acidophilus, Lactobacillus plantarum and bacillus bifidus at the temperature of 35 DEG C 13 days, obtain described mixed bacteria liquid, wherein, the weight proportion of strain and mixed-culture medium is 1:100, and the weight proportion of saccharomyces cerevisiae, bacillus acidophilus, Lactobacillus plantarum and bacillus bifidus is 10:25:20:55; ;
The preparation of the 3rd step, fermented product
(1) described composite plant is ground at least 40 object granules, with brown sugar and water mix and blend, obtain the pastel that ferments, wherein, the weight proportion of composite plant, brown sugar, pure water is 50:5:45, and described composite plant is 10 parts of Radix Puerariaes, 15 portions of Fructus Hippophaes and 75 parts of Semen Vitis viniferae;
(2) in described fermentation pastel, add saccharomyces cerevisiae liquid, sealed fermenting 5-8 days at the temperature of 29 DEG C, wherein, saccharomyces cerevisiae liquid is 1:20 with the weight proportion of fermentation pastel;
(3) then add the mixed bacteria liquid of bacillus acidophilus, Lactobacillus plantarum and bacillus bifidus, at the temperature of 34 DEG C, sealed fermenting 10-15 days, obtains fermented product, and wherein, mixed bacteria liquid is 1:20 with the weight proportion of fermentation pastel;
Prepared by the 4th step, concentrated solution
(1) add water in fermentation tank, make solid-to-liquid ratio reach 1:1, soak 1 day, then drip washing;
(2) leacheate carries out being concentrated into 20% at 50 DEG C;
(3) subsequently concentrated solution is carried out sterilizing at 60 DEG C of temperature, sterilization time is 20 minutes; Finally sterilized solution is preserved at 4 DEG C of temperature, stand-by;
The preparation of the 5th step, dosage form
Material is taken by following weight proportion: sterilized solution 20, erythritol 20, lactose 55, protective agent 5; First erythritol and lactose are added spraying dry one-step-granulating method successively; then start to spray into sterilized solution; flow 10ml/ minute; finally spray into the protective agent be dissolved in suitable quantity of water; the inlet temperature of granulator 60 DEG C; leaving air temp 45 DEG C, discharging when temperature drops to 30 DEG C in day with fog to be painted, obtains the solid formulation of described natural biological preparation.
Embodiment 2
A kind of preparation method of solid formulation of natural biological preparation:
The first step, the production production of hybrid seeds are with embodiment 1;
The preparation of second step, bacterium liquid
Brown sugar and water are mixed to get mixed-culture medium with the weight proportion of 7:93, be divided into two parts, a copy of it airtight cultivation saccharomyces cerevisiae 12 days at the temperature of 27 DEG C, obtain described saccharomyces cerevisiae liquid, the mixed bacteria of another part airtight cultivation bacillus acidophilus, Lactobacillus plantarum and bacillus bifidus at the temperature of 34 DEG C 15 days, obtain described mixed bacteria liquid, wherein, the weight proportion of strain and mixed-culture medium is 1:100, and the weight proportion of saccharomyces cerevisiae, bacillus acidophilus, Lactobacillus plantarum and bacillus bifidus is 10:20:20:60;
The preparation of the 3rd step, fermented product
(1) described composite plant is ground at least 40 object granules, with brown sugar and water mix and blend, obtain the pastel that ferments, wherein, the weight proportion of composite plant, brown sugar, pure water is 55:5:40, and described composite plant is 15 parts of Radix Puerariaes, 15 portions of Fructus Hippophaes and 70 parts of Semen Vitis viniferae;
(2) in described fermentation pastel, add saccharomyces cerevisiae liquid, sealed fermenting 5-8 days at the temperature of 30 DEG C, wherein, saccharomyces cerevisiae liquid is 1:15 with the weight proportion of fermentation pastel;
(3) then add the mixed bacteria liquid of bacillus acidophilus, Lactobacillus plantarum and bacillus bifidus, at the temperature of 34 DEG C, sealed fermenting 10-15 days, obtains fermented product, and wherein, mixed bacteria liquid is 1:15 with the weight proportion of fermentation pastel;
Prepared by the 4th step, concentrated solution
(1) add water in fermentation tank, make solid-to-liquid ratio reach 1:1, soak 1 day, then drip washing;
(2) leacheate carries out being concentrated into 20% at 45 DEG C;
(3) subsequently concentrated solution is carried out sterilizing at 65 DEG C of temperature, sterilization time is 10 minutes; Finally sterilized solution is preserved at 4 DEG C of temperature, stand-by;
The preparation of the 5th step, dosage form
Material is taken by following weight proportion: sterilized solution 45, erythritol 45, protective agent 10; First erythritol and protective agent are added appropriate hot distilled water stirring and dissolving, after being cooled to 35 DEG C, add sterilized solution, stir after solution is clarified, obtain the oral liquid of described natural biological preparation.
Application Example
Animal divides into groups: select healthy cleaning grade 70 age in days female SPF SD rat, body weight 240-300g, after conventional adaptation feeds 2 days, is divided into 4 groups at random, is respectively matched group, antioxidation group, damage model group and injury repairing group.
Experimental technique: matched group and damage model group are fed basal diet, antioxidant group and injury repairing group feed basal diet and in drinking-water by every every day 0.8mL add the oral liquid of the natural biological preparation that embodiment 2 prepares.Damage model group and injury repairing group press every kg body weight lumbar injection 1mgLPS respectively in the 5th, 9,13, the 17 day same time of test, and matched group and antioxidant group are at one time with the normal saline of same procedure injection equivalent.
Test point preliminary trial period 7d: basal diet of all feeding, freely drinks water; Just the phase of trying starts by EXPERIMENTAL DESIGN feeding experiment daily ration, drinking-water, injection LPS and normal saline.Measure the concentration that female Mus is just trying follicle stimulating hormone (FSH) and estradiol (E2) in the 14th day phase and the 22nd day serum.Data Processing in Experiment adopts the one factor analysis of variance of SPSS17.0 with analyzing, result represents with mean+SD.
Experimental result is in table 1:
The concentration of follicle stimulating hormone (FSH) and estradiol (E2) in female Mus serum respectively organized by table 1
As seen from the above table, the estradiol (E2) of antioxidant group is significantly higher than matched group, and injury repairing group concentration is also high than damage model group, and the effect that the oral liquid of natural biological preparation has enhancing estradiol (E2) and secretes is described.The oral liquid adding natural biological preparation improves female Mus levels of reproductive hormones, shows that the natural biological preparation that technical scheme of the present invention obtains has the effect that good improvement irritability climacteric shifts to an earlier date syndrome.
Be described in detail specific embodiments of the invention above, but it is just as example, the present invention is not restricted to specific embodiment described above.To those skilled in the art, any equivalent modifications that the present invention is carried out and substituting also all among category of the present invention.Therefore, equalization conversion done without departing from the spirit and scope of the invention and amendment, all should contain within the scope of the invention.
Claims (10)
1. one kind shifts to an earlier date the preparation method of the natural biological preparation of syndrome for improving irritability climacteric, it is characterized in that, a kind ofly obtain the preparation method of described natural biological preparation with the crushed mixture of composite plant for raw material is undertaken fermenting by complex microorganism, wherein:
The component of described composite plant and weight proportion thereof are 10-20 part Radix Puerariae, 10-20 part Fructus Hippophae and 50-80 part Semen Vitis viniferae;
The component of described complex microorganism and weight proportion thereof are 1-2 part yeast and 15-25 part lactobacillus, and wherein, described yeast is saccharomyces cerevisiae (Saccharomycescerevisiae, ACCC20065); Described lactobacillus is bacillus acidophilus (Lactobacilusacidophilus, ACCC10637), Lactobacillus plantarum (Lactobacilusplantarum, ACCC11118), the mixing of bacillus bifidus (Bifidobacterium, ACCC11054).
2. preparation method according to claim 1, is characterized in that, the weight proportion of bacillus acidophilus, Lactobacillus plantarum and bacillus bifidus is 2-3:2-3:5-10.
3. preparation method according to claim 1, it is characterized in that, described preparation method is specially:
Step 1: ground by described composite plant, with brown sugar and water mix and blend, obtains the pastel that ferments;
Step 2: add saccharomyces cerevisiae liquid in described fermentation pastel, sealed fermenting 5-8 days at the temperature of 25-30 DEG C;
Step 3: the mixed bacteria liquid then adding bacillus acidophilus, Lactobacillus plantarum and bacillus bifidus, sealed fermenting 10-15 days, obtains fermented product at the temperature of 30-37 DEG C.
4. preparation method according to claim 3, it is characterized in that, described preparation method also comprises the preparation of bacterium liquid, comprising: in the mixed-culture medium of brown sugar and water at the temperature of 25-30 DEG C airtight cultivation saccharomyces cerevisiae 10-15 days, obtain described saccharomyces cerevisiae liquid; In the mixed-culture medium of brown sugar and water at the temperature of 30-37 DEG C the mixed bacteria 10-15 days of airtight cultivation bacillus acidophilus, Lactobacillus plantarum and bacillus bifidus, obtain described mixed bacteria liquid.
5. preparation method according to claim 1-4 any one, is characterized in that, described preparation method also comprises carries out drip washing, concentrated, sterilization treatment successively to described fermented product, finally carries out the preparation of dosage form.
6. preparation method according to claim 1, it is characterized in that, described preparation method is:
Step 1: brown sugar and water are mixed to get mixed-culture medium with the weight proportion of 5-10:90-98, be divided into two parts, a copy of it is airtight cultivation saccharomyces cerevisiae 10-15 days at the temperature of 25-30 DEG C, obtain described saccharomyces cerevisiae liquid, the mixed bacteria 10-15 days of another part airtight cultivation bacillus acidophilus, Lactobacillus plantarum and bacillus bifidus at the temperature of 30-37 DEG C, obtain described mixed bacteria liquid, wherein, the weight proportion of strain and mixed-culture medium is 1:80-120;
Step 2: described composite plant is ground at least 30-50 object granule, with brown sugar and water mix and blend, obtain the pastel that ferments, wherein, the weight proportion of composite plant, brown sugar, pure water is 35-60:2-5:40-60;
Step 3: add saccharomyces cerevisiae liquid in described fermentation pastel, sealed fermenting 5-8 days at the temperature of 25-30 DEG C, wherein, saccharomyces cerevisiae liquid is 1:15-20 with the weight proportion of fermentation pastel;
Step 4: the mixed bacteria liquid then adding bacillus acidophilus, Lactobacillus plantarum and bacillus bifidus, at the temperature of 30-37 DEG C, sealed fermenting 10-15 days, obtains fermented product, and wherein, mixed bacteria liquid is 1:15-20 with the weight proportion of fermentation pastel;
Step 5: add water drip washing again after soaking in described fermented product, then carries out the 20-30% being concentrated into original volume at 40-50 DEG C of temperature by leacheate; Subsequently concentrated solution is carried out sterilizing at 60-70 DEG C of temperature, sterilization time is 10-30 minute, is finally preserved at 2-4 DEG C of temperature by sterilized solution;
Step 6: preparation dosage form, obtains described natural biological preparation.
7. preparation method according to claim 6, it is characterized in that, described dosage form comprises solid formulation, oral liquid, wherein:
By sterilized solution, sugar, protective agent with the weight proportion of 15-30:60-90:3-5, join successively in granulator, obtain described solid formulation, wherein, the flow of described granulator is 10-20ml/ minute, and inlet temperature is 60-80 DEG C, leaving air temp is 40-60 DEG C, and drop temperature is 25-35 DEG C;
Sterilized solution, sugar, protective agent are weighed with the weight proportion of 45-50:45-50:5-10, first dissolving adding suitable quantity of water in sugar and protective agent, adding sterilized solution after cooling, after stirring clarification, obtaining described oral liquid.
8. preparation method according to claim 7, is characterized in that, described sugar comprise in glucose, sucrose, fructose, lactose, erythritol, threitol, xylitol any one or a few.
9. preparation method according to claim 7, is characterized in that, described protective agent comprise in maltodextrin, corn starch, sucralose, citric acid any one or a few.
10. one kind prepares natural biological preparation by preparation method described in any one in claim 1-9.
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| CN110870878A (en) * | 2018-08-31 | 2020-03-10 | 生物节律有限公司 | Lactobacillus probiotic preparation for preventing or relieving female climacteric symptoms |
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Application publication date: 20151202 |