CN104026589B - A kind of peptide ferment and preparation method thereof - Google Patents
A kind of peptide ferment and preparation method thereof Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
A kind of peptide ferment and preparation method thereof, belongs to biology field, solves in the product of existing ferment preparation method existence the problem that ferment content is lower, preparation time long, useful number of viable is less.By 1000 parts of weight parts, this peptide ferment comprises following component: lucidum spore powder 10 ~ 100 parts; Lysozyme bacterium 20 ~ 50 parts; Cellulase bacterium 5 ~ 20 parts; Lactobacillus acidophilus 20 ~ 50 parts; Table sugar 150 ~ 250 parts; Vinegar 50 ~ 100 parts, all the other are purified water.The full sweat of the present invention is no more than 15 days, and the used time is short, and product is stablized, pollution-free miscellaneous bacteria, and in ferment product, ferment content is higher, and after fermentation, useful number of viable obviously increases.Be greater than under every milliliter of 3,000,000,000 ferment bacterium effects, large, middle-molecular-weihydroxyethyl protein in peptide ferment are turned to molecular weight below 1500 dalton by Peptides, can directly pass through intestinal mucosa small molecular weight protein directly absorbed by the body.
Description
Technical field
The invention belongs to technical field of molecular biology, be specifically related to a kind of peptide ferment and preparation method thereof.
Background technology
Enter 21 century, along with the improvement of global aging population and living standard, the cognition degree of people to health is more and more higher, has had a understanding progressively to diet structure, Sleep architecture, motion fit structure, particularly in recent years, people more and more pay attention to the importance of ferment in human body.Ferment (Enzyme) is also called " enzyme ", be a kind of be made up of amino acid there is special bioactive material, it is present in the animal and plant body of all work, is the required material of one maintaining the vital movements such as body normal function, digest food, repair tissue.It almost participates in all vital movements: ponder a problem, motion, and sleep is breathed, indignation, and cry or secretion hormone etc. is all the action result centered by ferment.The catalytic action of ferment catalyst urges the biochemical reaction of body, urges the carrying out of biological phenomena.If do not have ferment, biochemical reaction cannot carry out, and five large nutrients (carbohydrate, lipoids, proteins,vitamins,minerals) all will be utterly useless to body, and biological phenomena will stop.Therefore, the importance of ferment to life is self-evident, and it is called " material lived " by even a lot of people, " grasping the material of all vital movements ".
At present, known ferment has thousands of kinds, but scientist still cannot Prof. Du Yucang ferment, and human body ferment is mainly derived from self and manufactures and meal ingestion.Our health can manufacture more than more than 3000 kinds of ferment every day, but compared with the ferment that the biochemical reaction ten hundreds of with every day in body consumes, this is obviously far from being enough, and along with modern times various improper habits and customs and the growth at age, the ability that health manufactures ferment declines gradually, and therefore we must absorb more ferment from meals.But, ferment is to high temperature extreme sensitivity, the not high ferment that can destroy in food of temperature, want from food, obtain ferment and just must eat something rare food, but for Chinese, the result of the eating habit and excessively culinary art of eating prepared food completely makes health to the picked-up wretched insufficiency of ferment, invisible pressure is added to health, because ferment is to provide the nutrient of body energy, use the running limit also damaging health excessively, make the easy cancer stricken of health, obesity, angiocardiopathy, or become the host of Other diseases.When lacking ferment in body, biochemical reaction in body starts to slow down, health can feel fatigue for no reason, people often to support by force and worked on this time, or supplement food or the tonic of a little high nutrition, but this mode is obviously inadequate for maintenance physical function, this is not only nutrition and energy due to somagenic need, more it is desirable that body can be helped to use the material-ferment of these nutrition and energy, the generating object that Here it is why " overworking death " of current trend is all intellectual with a senior professional title's high salary personage, the dietary structure of obvious this kind of people is very perfect and have no lack of nutrition or even tonic, so overdraw ferment equals life of overdrawing, more should supplement ferment.
At present, existing ferment generally all adopts various assorted fruit mixing spontaneous fermentation to form, this sweat is easy to pollution microbes, and required fermentation time is longer, first time fermentation takes 2 months at least, and it is just ripe that ageing at least takes 6 months, and ferment content is lower in the rear ferment product that fermented, and the digestion inactivation be easy to through stomach, the quantity of rear useful viable bacteria of having fermented is also few, DeGrain, instability.
Summary of the invention
In order to solve the problem that in the product of existing ferment preparation method existence, ferment content is lower, preparation time long, the rear useful number of viable of fermentation is less, the invention provides a kind of peptide ferment and preparation method thereof.
The technical scheme that the present invention adopts for technical solution problem is as follows:
A kind of peptide ferment of the present invention, by 1000 parts of weight parts, this peptide ferment comprises following component:
Lucidum spore powder 10 ~ 100 parts;
Lysozyme bacterium 20 ~ 50 parts;
Cellulase bacterium 5 ~ 20 parts;
Lactobacillus acidophilus 20 ~ 50 parts;
Table sugar 150 ~ 250 parts;
Vinegar 50 ~ 100 parts, all the other are purified water.
Present invention also offers the method preparing above-mentioned peptide ferment, the method comprises the following steps:
(1) in fermentation tank, purified water 100 ~ 300 parts is added, the table sugar 150 ~ 250 parts of dissolving with hot water is added while stirring under 20 ~ 60r/min condition, lucidum spore powder 10 ~ 100 parts and lysozyme bacterium 20 ~ 50 parts is added again, temperature controlled fermentation 2 ~ 5 days under 35 ~ 40 DEG C of conditions after stirring;
(2) when effective viable bacteria content of step (1) gained Lysozyme in Solution bacterium be greater than 1,000,000,000 every milliliter, lysozyme bacterium produce that lysozyme activity is greater than 100U/ml, ganoderma lucidium spore powder wall breaking rate is greater than 80% time, vinegar 50 ~ 100 parts is added under 35 ~ 40 DEG C of conditions, cellulase bacterium 5 ~ 20 parts and lactobacillus acidophilus 20 ~ 50 parts is added again after stirring, activate 1 ~ 2 hour, supplement purified water to 1000 part;
In described step (2), after adding vinegar, solution ph should be less than 4.0, because now in solution, contained a large amount of its optimum pH of lysozyme bacterium is 6.0 ~ 8.0, the lactobacillus acidophilus added after just can not affecting be less than the condition of 4.0 in pH value under, cellulase bacteria growing, pH value 3.0 ~ 4.0 is just in time the optimum pH of lactobacillus acidophilus, cellulase bacterium simultaneously;
(3), after being stirred by step (2) gained solution, under 5 ~ 40 DEG C of conditions, anaerobic fermentation 7 ~ 10 days, stirs 1 ~ 2 hour every day;
(4) when viable bacteria content effective in step (3) gained solution be greater than 3,000,000,000 every milliliter, little peptide content is greater than 70%, pH value is 3.0 ~ 4.0, pol is 25% ~ 35% time, adopted by the solution in fermentation tank the filter of 0.4 μm of sieves to carry out filtration and obtain finished product peptide ferment.
Described table sugar is one or more in white sugar, brown sugar, rock sugar; Be preferably brown sugar, brown sugar is best to growth, product enzyme effect.
Described vinegar is one or more in rice vinegar, light-coloured vinegar, fruit vinegar; Be preferably light-coloured vinegar, light-coloured vinegar is best to lactobacillus acidophilus, cellulase bacteria growing effect.
Inventive principle:
Glossy ganoderma, the treasure of be just described as food and medicament dual-purpose since ancient times, promoting longevity, has and improves immunity, anti-ageing, effect such as control cardiovascular and cerebrovascular disease, Tumor suppression etc.Reishi sporule is the seed of glossy ganoderma, and in brown, avette, its medical value is high, and effect is equivalent to about 75 times of glossy ganoderma, and has dual regulation to human body, especially very remarkable to the preventive and therapeutic effect of hepatic disease, insomnia and aging.But Reishi sporule has double wall, outer sporoderm is smooth, and the two layers of walls of Reishi sporule parcel is very tough and tensile, and general chemistry, physical method are difficult to be broken, therefore the conidia powder of non-broken wall is difficult to the utilization that is absorbed by the body, and needs to carry out broken wall treatment to lucidum spore powder.The main component of Reishi sporule wall is chitin 52.05% ~ 57.64% and crude fibre 2.36% ~ 3.52%, first under lysozyme bacterium Liquid fermentation condition, and the chitinase produced by the fermentation of lysozyme bacterium, the chitin of enzymolysis Reishi sporule wall; Again under cellulase bacterium Liquid fermentation condition, by the cellulase that the fermentation of cellulase bacterium produces, the crude fibre of enzymolysis Reishi sporule wall, thus by ganoderma lucidium spore powder wall breaking, ganoderma lucidium spore powder wall breaking rate is greater than 80% and (samples by amplifying 1600 times of microscopies under electron microscope, whether broken wall, rupture of membranes and have outside inwardly projecting and tell phenomenon in field of view, calculate ratio and the broken wall percentage of exosporium-broken spore number and total spore count in picture after microphotograph).
Simultaneously, cellulase bacterium Liquid fermentation condition is also lactobacillus acidophilus the most adaptable method, by 7 ~ 10 days anaerobic fermentations, when viable bacteria content effective in solution is greater than 3,000,000,000 every milliliter, by the large molecular weight protein in the lucidum spore powder ferment solution (lucidum spore powder protein content 15% ~ 20%) of high protein content, fermentation peptide turns to molecular weight and is less than 1000 daltonian small molecular weight proteins, little peptide content is greater than 70%, and (phosphomolybdic acid can precipitate macromolecule and Middle molecule nitrogen substance simultaneously, low molecule nitrogen substance is not then precipitated by phosphomolybdic acid, therefore after the phosphomolybdic acid deposit sample recorded, the nitrogen content of gained filtrate is low molecule nitrogen content).
Drink ganoderma spove powder peptide ferment 1 ~ 3 time prepared by the present invention every day, each 20 ~ 30ml, effect is as follows:
1, decomposition: decomposition, digest food, make it to become absorbable material, promotes that muscle power is restored rapidly.
2, arrange vivo environment, purify the blood, improve physique; Decompose, get rid of discarded object, make blood become faintly acid, beautifying face and moistering lotion.
3, anti-inflammatory, antibacterial action: conveying white blood cell, promotes leukocytic function, promotes body self-healing ability.
4, anti-knurl anti-disease effect: with suitable medicine, nutritional preparation use, inspire drug effect, complement each other, and reduce side effect.
5, prevention seriously shave one's head, Bai Fa, dandruff.
6, just there is ferment in the ovum of human body and sperm, promote that sperm ovum binding is fertilized by these ferment, separately can bring back to life the gonad cell of women's decline, promote Reproductive Performance.
7, relieve the effect of alcohol, prevent being still drank after a night.
8, the child eating green vegetables fruit is not liked, best nutrition, energy source.
9, compose living cells: promote cell metabolism, increase thermal energy consumption, fat-reducing plastomer.
The invention has the beneficial effects as follows:
1, although in spore, material is easily absorbed by human body and skin after ganoderma lucidium spore powder wall breaking, but in spore, material is also oxidizable simultaneously, caking is rotten, this is because account in spore 20% oil droplet excessive and stick to conidial cell wall surface, by spore average diameter 8 μm, oil droplets is long-pending increases about 2500 times, as Oxidation of Fat and Oils speed is directly proportional (establish temperature conditions identical) with grease surface area × period of storage, surface area increases 2500 times, period of storage then should reduce 2500 times, suppose that grease stores and rotten has oil consumption taste in 1 year, then after breaking trachytectum 4h namely because Oxidation of Fat and Oils produces oil consumption taste, so anti-oxidation process should be done after broken wall.Therefore, after ganoderma lucidium spore powder wall breaking, adopt which kind of effective anti-oxidant measure to be the key of producing ganoderma spove powder health products.The present invention fully takes into account this point in technological design, the ferment product prepared according to the present invention, under high pol condition, make full use of lysozyme bacterium froth breaking, specific function that anticorrosion and anaerobism lactobacillus acidophilus, cellulase bacterium effectively dispel oxygen radical, antioxidation is remarkable.
The probability of 2, traditional ferment sweat pollution microbes is large, and required time is long, take the first time fermentation of 2 months at least, comprising ageing, at least to take 6 months just ripe, and effective bacterium that the present invention takes Substratspezifitaet strong carries out single fermentation to this relatively general powerful substrate of plant of lucidum spore powder, full sweat is no more than 15 days, and the time is short and the simple employment of equipment is few, product is stable does not almost have pollution microbes possibility, is suitable for large-scale production.
3, the glossy ganoderma acid containing bitter taste in ganoderma spove powder, taste is poor, and the daily census of population's result taken has 10 ~ 20% to occur constipation phenomenon.The peptide ferment produced according to this patent method, macro-molecular protein in solution is degraded to polypeptide, little peptide and free amino acid by ferment bacterium, effective passivation ANFs is active, generate the materials such as a large amount of probios, lactic acid, UGF simultaneously, separately need not add artificial color, chemical spices and anticorrisive agent, color is soft, mouthfeel is good, treatment constipation.
4, under the effect being greater than every milliliter of 3,000,000,000 ferment bacterium, large, middle-molecular-weihydroxyethyl protein in peptide ferment are turned to molecular weight below 1500 dalton by Peptides, can directly pass through intestinal mucosa small molecular weight protein directly absorbed by the body.Absorption power consumption is low, speed fast, and not easily saturated, do not exist and compete with Amino Acid Absorption, can also promote the transhipment of free amino acid, protein digestibility is up to more than 99%.
5, in ferment production process, by ferment bacterium, macromolecule protein peptide being changed into the ability of small molecular protein---small molecular protein content detection is as the leading indicator appraised the quality of a product, and more general only foundation sense organ, bacterium number identification method are more accurate.
6, the major function of lucidum spore powder and ferment bacterium is all improve the S0D growing amount of human body, eliminates free radical, bidirectional modulation endocrine, improve the physiological function of cell, improve liver, marrow, blood synthesis, the ability of DNA, RNA, protein, the effects such as radioresistance, aging, fatigue.Both complement each other, lucidum spore powder has extraordinary growth promoting effects effect to ferment bacterium, ferment bacterium is then effectively accelerated lucidum spore powder and is absorbed by the body, the peptide ferment product produced thus, be that one is ill to cure the disease, without two property adjustment functions for the treatment of diseases and making health-care, without any side effect and dependence, men and women, old and young are edible, is optimal natural health care of promoting longevity.
Accompanying drawing explanation
Fig. 1 is Reishi sporule micrograph in ferment solution before broken wall.
Fig. 2 is Reishi sporule micrograph in ferment solution after broken wall.
Detailed description of the invention
Lysozyme bacterium used in the present invention, lactobacillus acidophilus, cellulase bacterium three strain bacterial strain are purchased from Institute of Microorganism, Academia Sinica, carry out seed selection, expand and cultivate by Jilin University's Life Science College.
Embodiment 1
To acid resistance, add purified water 100 parts in the fermentation tank of band temperature control, agitating function, adopt the mixer of rotating speed 20r/min, add 150 parts, the brown sugar dissolved with hot water while stirring, lucidum spore powder 10 parts is added again, lysozyme bacterium 20 parts, 35 DEG C of temperature controlled fermentations 2 days after stirring, when effective viable bacteria content of Lysozyme in Solution bacterium is greater than 1,000,000,000 every milliliter, lysozyme bacterium produces lysozyme activity and is greater than 100U/ml, when ganoderma lucidium spore powder wall breaking rate is greater than 80%, 35 DEG C add light-coloured vinegar 50 parts, cellulase bacterium 5 parts is added again after stirring, lactobacillus acidophilus 20 parts, activate 1 hour, supplement purified water to 1000 part, after stirring, room temperature anaerobic fermentation 10 days, stir 1 hour every day, when viable bacteria content effective in solution is greater than 3,000,000,000 every milliliter, little peptide content is greater than 70%, pH value 4.0, during pol 25%, adopt the filter of sieves 0.4 μm to carry out filtration the solution in fermentation tank and obtain finished product peptide ferment.
Embodiment 2
To acid resistance, add purified water 200 parts in the fermentation tank of band temperature control, agitating function, adopt the mixer of rotating speed 30r/min, add 200 parts, the brown sugar dissolved with hot water while stirring, lucidum spore powder 50 parts is added again, lysozyme bacterium 30 parts, 38 DEG C of temperature controlled fermentations 3 days after stirring, when effective viable bacteria content of Lysozyme in Solution bacterium is greater than 1,000,000,000 every milliliter, lysozyme bacterium produces lysozyme activity and is greater than 100U/ml, when ganoderma lucidium spore powder wall breaking rate is greater than 80%, 38 DEG C add light-coloured vinegar 80 parts, cellulase bacterium 10 parts is added again after stirring, lactobacillus acidophilus 30 parts, activate 2 hours, supplement purified water to 1000 part, after stirring, room temperature anaerobic fermentation 8 days, stir 2 hours every day, when viable bacteria content effective in solution is greater than 3,000,000,000 every milliliter, little peptide content is greater than 70%, pH value 3.5, during pol 30%, adopt the filter of sieves 0.4 μm to carry out filtration the solution in fermentation tank and obtain finished product peptide ferment.
Embodiment 3
To acid resistance, add purified water 300 parts in the fermentation tank of band temperature control, agitating function, adopt the mixer of rotating speed 60r/min, add 250 parts, the brown sugar dissolved with hot water while stirring, lucidum spore powder 100 parts is added again, lysozyme bacterium 50 parts, 40 DEG C of temperature controlled fermentations 5 days after stirring, when effective viable bacteria content of Lysozyme in Solution bacterium is greater than 1,000,000,000 every milliliter, lysozyme bacterium produces lysozyme activity and is greater than 100U/ml, when ganoderma lucidium spore powder wall breaking rate is greater than 80%, 40 DEG C add light-coloured vinegar 100 parts, cellulase bacterium 20 parts is added again after stirring, lactobacillus acidophilus 50 parts, activate 2 hours, supplement purified water to 1000 part, after stirring, room temperature anaerobic fermentation 7 days, stir 1 hour every day, when viable bacteria content effective in solution is greater than 3,000,000,000 every milliliter, little peptide content is greater than 70%, pH value 3.2, during pol 35%, adopt the filter of sieves 0.4 μm to carry out filtration the solution in fermentation tank and obtain finished product peptide ferment.
The peptide ferment product that test example 1 is prepared by embodiment 2, detect through Jilin Province's product quality supervision and testing institute, its technical indicator is as shown in table 1:
Table 1
| Interventions Requested | Assay | Interventions Requested | Assay | Interventions Requested | Assay |
| Crude protein | 5.286％ | Lactic acid | 3％ | Pb | 0.01mg/L |
| Crude fat | 2.237％ | Total reducing sugar | 32％ | SO 2 | 2.78mg/L |
| Crude fibre | 8.45％ | Cu | 2.17mg/L | Salmonella | Do not detect |
| Coarse ash | 1.27％ | Taurine | 6.32mg 0g -1 | Escherichia coli | Do not detect |
| Small-molecular peptides | 75％ | As | 0.05mg/L | Shigella | Do not detect |
| Ferment bacterium | 3.5×10 10cfu/mL | Soluble solid | 45％ | Staphylococcus aureus | Do not detect |
Table 2: peptide ferment vitamin content (unit: mg/100g)
Table 3: peptide ferment amino acid content (unit: g/100g)
Above Testing index meets the quality standard (GB/T21732-2008) of milk-contained drink completely, absolutely proves that the most important index of the crude protein content of peptide ferment, little peptide content, ferment bacterium content, lactic acid content, amino acid content these several qualification ferment product is all higher than existing commercially available ferment product by above testing inspection index.
Observe under 100 power microscopes, Fig. 1 is non-exosporium-broken spore is avette, and as shown in Figure 2, substantially do not observe complete spore shape under microscope in the ferment solution after lysozyme broken wall, spore ultrastructure disintegrates substantially, forms more uniform disrupting tissue.
Claims (5)
1. a peptide ferment, is characterized in that, by 1000 parts of weight parts, this peptide ferment is prepared by following component:
Lucidum spore powder 10 ~ 100 parts;
Lysozyme bacterium 20 ~ 50 parts;
Cellulase bacterium 5 ~ 20 parts;
Lactobacillus acidophilus 20 ~ 50 parts;
Table sugar 150 ~ 250 parts;
Vinegar 50 ~ 100 parts, all the other are purified water;
The preparation method of this peptide ferment, comprises the following steps:
(1) in fermentation tank, purified water 100 ~ 300 parts is added, the table sugar 150 ~ 250 parts of dissolving with hot water is added while stirring under 20 ~ 60r/min condition, lucidum spore powder 10 ~ 100 parts and lysozyme bacterium 20 ~ 50 parts is added again, temperature controlled fermentation 2 ~ 5 days under 35 ~ 40 DEG C of conditions after stirring;
(2) when effective viable bacteria content of step (1) gained Lysozyme in Solution bacterium be greater than 1,000,000,000 every milliliter, lysozyme bacterium produce that lysozyme activity is greater than 100U/ml, ganoderma lucidium spore powder wall breaking rate is greater than 80% time, vinegar 50 ~ 100 parts is added under 35 ~ 40 DEG C of conditions, cellulase bacterium 5 ~ 20 parts and lactobacillus acidophilus 20 ~ 50 parts is added again after stirring, activate 1 ~ 2 hour, supplement purified water to 1000 part;
(3), after being stirred by step (2) gained solution, under 5 ~ 40 DEG C of conditions, anaerobic fermentation 7 ~ 10 days, stirs 1 ~ 2 hour every day;
(4) when viable bacteria content effective in step (3) gained solution be greater than 3,000,000,000 every milliliter, little peptide content is greater than 70%, pH value is 3.0 ~ 4.0, pol is 25% ~ 35% time, adopted by the solution in fermentation tank the filter of 0.4 μm of sieves to carry out filtration and obtain finished product peptide ferment.
2. a kind of peptide ferment according to claim 1, is characterized in that, described table sugar is one or more in white sugar, brown sugar, rock sugar.
3. a kind of peptide ferment according to claim 2, is characterized in that, described table sugar is brown sugar.
4. a kind of peptide ferment according to claim 1, is characterized in that, described vinegar is one or more in rice vinegar, light-coloured vinegar, fruit vinegar.
5. a kind of peptide ferment according to claim 4, is characterized in that, described vinegar is light-coloured vinegar.
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