CN104286827B - Method for fermenting ganoderma lucidum spore powder by using probiotics - Google Patents
Method for fermenting ganoderma lucidum spore powder by using probiotics Download PDFInfo
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- CN104286827B CN104286827B CN201410470958.7A CN201410470958A CN104286827B CN 104286827 B CN104286827 B CN 104286827B CN 201410470958 A CN201410470958 A CN 201410470958A CN 104286827 B CN104286827 B CN 104286827B
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/40—Shaping or working of foodstuffs characterised by the products free-flowing powder or instant powder, i.e. powder which is reconstituted rapidly when liquid is added
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Abstract
The invention discloses a method for fermenting ganoderma lucidum spore powder by using probiotics. The method comprises the following steps: carrying out liquid-state fermentation on the ganoderma lucidum spore powder with bacillus subtilis and lactobacillus acidophilus; and then, freezing, spraying and drying to obtain the product. The method has the beneficial effects that (1) the wall breaking of the ganoderma lucidum spore powder is realized and the wall breaking efficiency is more than 85%; the absorption rate is improved; (2) after probiotics fermentation, the content of nutritional components including protein, vitamins, polysaccharides and the like is increased so that the nutritional value of foods is greatly improved; (3) after the fermentation, the quantity of the probiotics in a product is great and the pH is low; the product has a certain antiseptic effect; (4) the product has a rich lactic acid flavor and is mellow and full, and smooth when being eaten; and (5) a step of freezing, spraying and drying is adopted so that functional components in the ganoderma lucidum spore powder are protected, the quantity and the activity of the probiotics in the product are maintained to the greatest extent, the balance of intestinal flora of users is benefited, and the health effect of the ganoderma lucidum spore powder is strengthened.
Description
Technical field
The present invention relates to a kind of method of utilization probiotics fermention Ganoderma spore powder.
Background technology
Ganoderma spore powder is the spore being discharged when Ganoderma is ripe, is Ganoderma sexual reproductive cell, also known as basidiospore.Ganoderma
Spore powder is in terms of suppression tumor, enhancing immunity considerably beyond Ganoderma sporophore.There is document to Ganoderma sporophore and spore powder
Composition carried out analysis contrast, result shows that the crude protein content of the latter is approximately the former twice, before crude fat content is approximately
5 times of person.Ganoderma spore brown, avette, one end is truncate, (8.5-1.12) um × (5.2-6.9) um, has double wall.Ganoderma spore
Sub- wall is solid hard, and acid and alkali-resistance, it is extremely difficult to oxidation Decomposition, therefore limits people and active substance in spore is digested and assimilated, be
Make full use of the active substance in Ganoderma spore it is necessary to breaking cellular wall is carried out to spore powder.There is document report, after breaking cellular wall, human body pair
The absorbance of active substance can improve as many as 45 times.
Probiotic bacteria (probiotics) is that a class is beneficial to a class of host by improving host's intestinal microecology balance
Microorganism, is the important physiology bacterium of human body intestinal canal.The ecological balance of microorganism species in intestinal can be promoted, suppress harmful bacteria
Growth, enhancing human body immunity power, reduction cholesterol, elimination cancerigenic factor, chronic for the " three high " threatening modern humans' health
The preventing and treating important in inhibiting of disease and cancer.
Edible safety that bacillus subtilises and bacillus acidophilus are well recognized as, the microorganism beneficial to human body.Hay spore
Bacillus has powerful enzyme system, and through experiment, it can be used for carrying out biological wall breaking to Ganoderma spore powder, but culture has a poor flavour.
After fermenting further through bacillus acidophilus, its local flavor becomes plentiful pleasant.The present invention is using mixed bacterium stage by stage to Ganoderma spore
Sub- powder is fermented, both can breaking cellular wall, its nutritional labeling can be enriched again, both can be anti-corrosion, its flavour of food products can be improved again.After fermentation
Ganoderma spore powder there is strong lactic acid local flavor, the not good defect of occurring spore powder mouthfeel can be overcome, using cryospray do
Drying process saves the probiotic bacteria of considerable amount, is effectively protected the active substance in Ganoderma spore powder simultaneously.
Content of the invention
The technical problem to be solved in the present invention is to provide a kind of method of utilization probiotics fermention Ganoderma spore powder, the present invention
Overcome Ganoderma spore powder some shortcomings in healthcare applications, such as nutrition is difficult to absorb, mouthfeel is poor, part population occurs just
The problems such as secret side effect, it is simultaneously achieved the physiological function of probiotic bacteria and the health-care effect perfect adaptation of Ganoderma spore powder, simultaneously
The present invention is fermented to Ganoderma spore powder using bacillus subtilises and bacillus acidophilus, and two bacterial strains have reliable safety
Property, stronger lactic acid produce ability and good probiotic effects.
For solving the above problems, the present invention adopts the following technical scheme that
A kind of method of utilization probiotics fermention Ganoderma spore powder, including following preparation process:
1) prepare 10% ~ 20%(mass fraction) Ganoderma spore powder suspension, add 0.5% ~ 1.0%(mass fraction) breast
Sugar, balance of water, put in fermentation tank, then put into the first nutrient chemical again, after the completion of interpolation, keep cultivation temperature 32 DEG C ~ 36
DEG C, speed of agitator is 120r/min ~ 160r/min, ventilation 0.4 ~ 0.7vvm；
2) add lactose solution in obtained culture in step 1), so that the lactose concn in culture is improved
0.5%；
3) to step 2) middle input the second nutrient chemical, then keep 32 DEG C ~ 38 DEG C of cultivation temperature again, close ventilation and stir
Mix, static fermentation 16 ~ 22h；
4) obtained culture in step 3) is carried out cryospray drying, -25 DEG C ~ -20 DEG C of spray chilling temperature, spray
Mist pressure 3bar ~ 5bar；
5) culture completing to spraying in step 4) carries out subpackage, obtains final product product.
As preferred technical scheme, described first nutrient chemical is bacillus subtilis bacteria culture fluid (adding proportion 5% ~ 8%)
Or bacillus subtilises mycopowder (adding proportion 0.1% ~ 0.3%).
As preferred technical scheme, described second nutrient chemical be bacillus acidophilus' culture fluid (adding proportion 5% ~ 8%) or
Bacillus acidophilus' mycopowder (adding proportion 0.1% ~ 0.3%).
The invention has the beneficial effects as follows:
1) achieve the breaking cellular wall of Ganoderma spore powder, sporoderm-broken rate more than 85% so as to effective ingredient is more easily absorbed by the body,
Improve absorbance；
2) after probiotics fermention, the nutrient composition content such as its protein, vitamin, polysaccharide is all increased, thus
Nutritive value of food is made to greatly improve；
3) fermented, probiotic bacteria substantial amounts in product, and ph is relatively low, is unfavorable for the growth of other microorganisms, itself
There is certain antiseptic effect；
4) this product has strong lactic acid local flavor, and entrance is mellow and full smooth, and the Common ganoderma lucidum spore powder of more slightly bitterness has
Obvious advantage；
5) adopt cryospray to be dried, in protection Ganoderma spore powder while functional component, remain product to greatest extent
The quantity of probiotic bacteria and activity in product, are beneficial to the intestinal microbial population balance of user, the health-care effect of strengthening Ganoderma spore powder.
Specific embodiment
Below the preferred embodiments of the present invention are described in detail so that advantages and features of the invention can be easier to by
It will be appreciated by those skilled in the art that apparent clearly defining thus making to protection scope of the present invention.
A kind of method of utilization probiotics fermention Ganoderma spore powder, including following preparation process:
1) prepare 10% ~ 20%(mass fraction) Ganoderma spore powder suspension, add 0.5% ~ 1.0%(mass fraction) breast
Sugar, balance of water, put in fermentation tank, then put into the first nutrient chemical again, after the completion of interpolation, keep cultivation temperature 32 DEG C ~ 36
DEG C, speed of agitator is 120r/min ~ 160r/min, ventilation 0.4 ~ 0.7vvm；
2) add lactose solution in obtained culture in step 1), so that the lactose concn in culture is improved
0.5%；
3) to step 2) middle input the second nutrient chemical, then keep 32 DEG C ~ 38 DEG C of cultivation temperature again, close ventilation and stir
Mix, static fermentation 16 ~ 22h；
4) obtained culture in step 3) is carried out cryospray drying, -25 DEG C ~ -20 DEG C of spray chilling temperature, spray
Mist pressure 3bar ~ 5bar；
5) culture completing to spraying in step 4) carries out subpackage, obtains final product product.
Described first nutrient chemical is bacillus subtilis bacteria culture fluid (adding proportion 5% ~ 8%) or bacillus subtilises mycopowder
(adding proportion 0.1% ~ 0.3%).
Described second nutrient chemical is bacillus acidophilus' culture fluid (adding proportion 5% ~ 8%) or bacillus acidophilus' mycopowder (adds
Ratio 0.1% ~ 0.3%).
The invention has the beneficial effects as follows:
1) achieve the breaking cellular wall of Ganoderma spore powder, sporoderm-broken rate more than 85% so as to effective ingredient is more easily absorbed by the body,
Improve absorbance；
2) after probiotics fermention, the nutrient composition content such as its protein, vitamin, polysaccharide is all increased, thus
Nutritive value of food is made to greatly improve；
3) fermented, probiotic bacteria substantial amounts in product, and ph is relatively low, is unfavorable for the growth of other microorganisms, itself
There is certain antiseptic effect；
4) this product has strong lactic acid local flavor, and entrance is mellow and full smooth, and the Common ganoderma lucidum spore powder of more slightly bitterness has
Obvious advantage；
5) adopt cryospray to be dried, in protection Ganoderma spore powder while functional component, remain product to greatest extent
The quantity of probiotic bacteria and activity in product, are beneficial to the intestinal microbial population balance of user, the health-care effect of strengthening Ganoderma spore powder.
Embodiment 1
Ganoderma spore powder 10%, Lactose 0.6%, accesses 5% bacillus subtilis bacteria culture fluid, 34 DEG C, 120r/min, ventilation
0.5vvm, cultivates 12h.Add 0.5% Lactose in fermentation liquid, access 5% bacillus acidophilus' culture fluid, 34 DEG C of quiescent culture 18h.
Setting -20 DEG C of spray chilling temperature, atomisation pressure 3bar, by fermentation liquid carry out cryospray dry fermentation glossy ganoderma spore powder.
After tested, present invention achieves the breaking cellular wall of Ganoderma spore powder, sporoderm-broken rate 89% is so as to effective ingredient is more easy to as human body
Absorb, improve absorbance.
Embodiment 2
Ganoderma spore powder 15%, Lactose 0.8%, accesses 0.2% bacillus subtilises mycopowder, 36 DEG C, 150r/min, ventilation
0.6vvm, cultivates 12h.Add 0.5% Lactose in fermentation liquid, access 0.3% bacillus acidophilus' mycopowder, 37 DEG C of quiescent culture 20h.
Setting -25 DEG C of spray chilling temperature, atomisation pressure 4bar, by fermentation liquid carry out cryospray dry fermentation glossy ganoderma spore powder.
After tested, present invention achieves the breaking cellular wall of Ganoderma spore powder, sporoderm-broken rate 95% is so as to effective ingredient is more easy to as human body
Absorb, improve absorbance.
The above, the only specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, and any
The change or replacement expected without creative work, all should be included within the scope of the present invention.Therefore, the present invention
Protection domain should be defined by the protection domain that claims are limited.
Claims (1)
1. a kind of method of utilization probiotics fermention Ganoderma spore powder is it is characterised in that include following preparation process:
1) with water prepare Ganoderma spore powder mass fraction be 10%～20% Ganoderma spore powder suspension, add Lactose so that
Mass fraction in Ganoderma spore powder suspension for the Lactose is 0.5%～1.0%, puts in fermentation tank, then puts into first again
Nutrient chemical, after the completion of interpolation, keeps 32 DEG C～36 DEG C of cultivation temperature, and speed of agitator is 120r/min～160r/min, ventilation
0.4～0.7vvm；
2) to step 1) in add lactose solution in obtained culture, make the lactose concn in culture improve 0.5%；
3) to step 2) middle input the second nutrient chemical, then keep 32 DEG C～38 DEG C of cultivation temperature again, close ventilation and stir,
Static fermentation 16～22h；
4) by step 3) in obtained culture carry out cryospray drying, -25 DEG C～-20 DEG C of spray chilling temperature, spraying
Pressure 3bar～5bar；
5) to step 4) in the culture that completes of spraying carry out subpackage, obtain final product product,
Described first nutrient chemical is bacillus subtilis bacteria culture fluid or bacillus subtilises mycopowder；
Described second nutrient chemical is bacillus acidophilus' culture fluid or bacillus acidophilus' mycopowder.
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| CN201410470958.7A CN104286827B (en) | 2014-09-16 | 2014-09-16 | Method for fermenting ganoderma lucidum spore powder by using probiotics |
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| CN201410470958.7A CN104286827B (en) | 2014-09-16 | 2014-09-16 | Method for fermenting ganoderma lucidum spore powder by using probiotics |
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| CN104286827B true CN104286827B (en) | 2017-01-18 |
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| CN109123611A (en) * | 2018-08-03 | 2019-01-04 | 安徽康宸药业有限公司 | A kind of preparation method of the ganoderma spove powder electuary with strengthen immunity and anti-oxidation function |
| CN110882287A (en) * | 2019-12-31 | 2020-03-17 | 合肥康春堂药业有限责任公司 | Ganoderma lucidum fruiting body microbial fermentation preparation for treating intestinal flora disorder and intestinal barrier function impairment |
| CN112913968A (en) * | 2021-02-04 | 2021-06-08 | 王会宁 | Liquid ganoderma lucidum feed and preparation method thereof |
Citations (8)
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| CN1101860A (en) * | 1993-10-21 | 1995-04-26 | 谭礼 | Process for producing biochemical raw material of spore powder of glossy ganoderma |
| CN1740327A (en) * | 2005-09-23 | 2006-03-01 | 福州恒丰量子生物科技有限公司 | Animal plant and microbial cell wall lytic enzyme reactive liquid and its application |
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| CN103300275A (en) * | 2013-06-24 | 2013-09-18 | 北京知蜂堂蜂产品有限公司 | Royal jelly and preparation method thereof |
| CN103933080A (en) * | 2014-04-17 | 2014-07-23 | 四川农业大学 | Preparation of Bacillussubtilis BS01 fermented ganoderma lucidum spore powder and application thereof in sleep improvement |
| CN104026589A (en) * | 2014-06-16 | 2014-09-10 | 吉林省丰润能源开发有限公司 | Peptide phenoloxidase as well as preparation method thereof |
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2014
- 2014-09-16 CN CN201410470958.7A patent/CN104286827B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1101860A (en) * | 1993-10-21 | 1995-04-26 | 谭礼 | Process for producing biochemical raw material of spore powder of glossy ganoderma |
| CN1919216A (en) * | 2005-08-24 | 2007-02-28 | 上海亘卓生物工程有限公司 | Microbiological antioxidant and method for preparing same |
| CN1740327A (en) * | 2005-09-23 | 2006-03-01 | 福州恒丰量子生物科技有限公司 | Animal plant and microbial cell wall lytic enzyme reactive liquid and its application |
| CN101596220A (en) * | 2009-04-07 | 2009-12-09 | 西藏月王生物技术有限公司 | A kind of wall-breaking method of Ganoderma spore |
| CN201589495U (en) * | 2010-01-18 | 2010-09-22 | 山东天力干燥设备有限公司 | Multifunctional intermittent drying system of freeze-spray granulation fluidized bed |
| CN103300275A (en) * | 2013-06-24 | 2013-09-18 | 北京知蜂堂蜂产品有限公司 | Royal jelly and preparation method thereof |
| CN103933080A (en) * | 2014-04-17 | 2014-07-23 | 四川农业大学 | Preparation of Bacillussubtilis BS01 fermented ganoderma lucidum spore powder and application thereof in sleep improvement |
| CN104026589A (en) * | 2014-06-16 | 2014-09-10 | 吉林省丰润能源开发有限公司 | Peptide phenoloxidase as well as preparation method thereof |
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Denomination of invention: A method for fermentation of ganoderma lucidum spore powder with probiotics Effective date of registration: 20221122 Granted publication date: 20170118 Pledgee: Zhejiang Tailong Commercial Bank Co.,Ltd. Hangzhou Fuyang sub branch Pledgor: ZHEJIANG YALIN BIOTECHNOLOGY Co.,Ltd. Registration number: Y2022980022630 |