CN107920575A - A kind of manufacture method of Synbiotics tunning and the Synbiotics tunning thus manufactured - Google Patents
A kind of manufacture method of Synbiotics tunning and the Synbiotics tunning thus manufactured Download PDFInfo
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- CN107920575A CN107920575A CN201680050533.8A CN201680050533A CN107920575A CN 107920575 A CN107920575 A CN 107920575A CN 201680050533 A CN201680050533 A CN 201680050533A CN 107920575 A CN107920575 A CN 107920575A
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- synbiotics
- tunning
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
Abstract
Manufacture method and the Synbiotics tunning that thus manufactures the present invention relates to a kind of Synbiotics tunning, more particularly to a kind of mixture that mixed lactic bacteria culture fluid is inoculated in beans and oat ferments, the Synbiotics tunning crushed using low speed grinding stone.The manufacture method of the Synbiotics tunning of the present invention, nutrient source as probiotics uses the prebiotics (prebiotics) that mixing beans and parkin are crushed using low speed grinding stone, therefore the size that obtained Synbiotics tunning includes water-soluble colloid form is less than 0.2um and the beta glucan particle stablized to temperature and acid, so as to can be developed into maintenance health, strengthen immunity, the healthy food of pre- anti-cancer and assistant treating cancer effect.
Description
Technical field
Manufacture method and the Synbiotics tunning that thus manufactures the present invention relates to a kind of Synbiotics tunning, especially
It is related to a kind of mixture that mixed lactic bacteria culture fluid is inoculated in beans and oat to ferment, is crushed using low speed grinding stone
Synbiotics tunning.
Background technology
Probiotics (probiotics) is live body micro- life of the expression with the healthy effect beneficial to hosts such as human or animals
The term of thing or its component, the benefit such as bacterium in balanced enteron aisle is provided to absorb the host of probiotics.In general, prebiotic bacterium bag
The scope of the beneficial bacteriums such as lactic acid bacteria or Bifidobacterium and yeast is included, wherein more research and use belong to Bacillus acidi lactici
(Lactobacillus), galactococcus (Lactococcus), Bifidobacterium (Bifidobacteria), streptococcus
(Streptococcus) etc. the lactic acid bacteria of (genus) is belonged to.
Lactic acid bacteria (LAB, lactic acid bacteria) is GRAS (generally recognized as safe),
It is long-term in history to be used to make various fermented foods.Lactic acid bacteria is used as matrix using the various sugar including lactose (lactose)
Lactic acid (lactic acid) is converted into, and process in this way assigns tart flavour to food, pH value is reduced, so as to suppress
Harmful microbe is grown.Lactic acid bacteria not only has antibacterial effect, but also in the intestinal microflora by host
(microflora) suppressing each side such as various intestines problems, strengthen immunity has to people's beneficial effect, so that by breast
Sour bacterium exploitation is of considerable interest into various food materials aspect.
Lactic acid bacteria is most representational probiotics, centered on fermented dairy product, in various fermented foods, sauce class, doctor
Medicine, the feed addictive etc. of domestic animal are widely used in the life of the mankind.Recently it is right in addition to the nutritive validity of lactic acid bacteria
Emphasize that lactic acid bacteria is carried out very active in the research of the various healthy functions of the enteral of host.
To strengthen the intravital activity of above-mentioned lactic acid bacteria, the probiotics of living cells and the battalion as them are belonged to mixing
The research of the Synbiotics (synbiotics) of the prebiotics (prebiotics) in the source of supporting is carried out very active.But for existing
There is the prebiotics of the Synbiotics of technology too many because being digested in the digestive organs of host, so that the nutrition supply source as probiotics
Cannot play the role of it is effective, be additionally, since prebiotics species specificity deficiency, then need to increase the activity of extraordinary lactic acid bacteria
When, it can not play the role of effective.
Beta glucan is the carbohydrate macromolecule polysaccharide for being connected with glucose molecule, is to be present in mushroom class, yeast
Cell membrane, cereal etc. have the material for strengthening immune and anticancer effect, in the 1940's as the cell membrane participation in yeast
The material of immune response is found first.Beta glucan is one kind of polysaccharide, in the material as the cell membrane of composition yeast
A kind of beta glucan (glucan) be the homopolysaccharide (homopolysaccharide) being made of glucose one kind, exist
In the internal layer (inner layer) of the cell membrane of yeast.
The propagation and recurrence, enhancement that can promote the immunological function repression cancer cell by activating the normal cell tissue of people are exempted from
The generation of the interleukin (interleukin), interferon (interferon) of the function of epidemic disease cell.Active beta-glucans, which enter, to be deposited
In the internal production cell factor (Cytokine) of cancer cell, support the activity of the T cell and B cell as immunocyte, rise
To the effect of the immune function of activating cell tissue.
The structure that to be glucose be made of -1,3 pillar (backbone) combined being most widely known by the people in beta glucan, root
There is the difference combined according to different structures, and the difference of such combination has close contact with metabolism and whole activity.But
It is that the different beta glucan of integrated structure there may exist difference in the species or its degree of activity.Present South Korea is domestic raw
The beta glucan of production is largely produced in addition to the cell of micro-organisms, using polysaccharide by large-scale fermentation tank, is produced
Beta glucan is glucose by β -1, and macromolecule that 3- is bonded is insoluble to water.In order to make while possessing physiological activity
It is that a variety of materials is used, it is necessary to which macromolecule beta glucan is converted to water-soluble beta glucan.In order to possess physiological activity
Used at the same time as a variety of materials, develop the technology that macromolecule beta glucan is converted to water-soluble beta glucan, and it is above-mentioned
The method of water-solubleization has carboxymethyl method, method of sulfonating, oligomerization method etc..
The content of the invention
Problems to be solved by the invention
A kind of mix is provided and is gathered comprising the β-Portugal easily absorbed it is an object of the invention to overcome the deficiency of the prior art
Synbiotics (synbiotics) tunning of the prebiotics (prebiotics) of the probiotics of sugar and the nutrient source as them
Manufacture method.
Another object of the present invention is to provide by Synbiotics produced by the present invention (synbiotics) tunning.
Solution to the problem
To reach above-mentioned purpose, the present invention provides a kind of manufacture method of Synbiotics tunning, including：
The step of cleaning beans and oat；
The beans of above-mentioned cleaning and oat, oat and the immersion of 150~250 parts by weight are mixed relative to the beans of 100 parts by weight
After in water, at 10~25 DEG C maintain 10 it is small when more than the step of；
By the temperature of water improve to more than 80 DEG C and maintain 30 minutes~1 it is small when the step of；
Relative to 100 parts by weight of beans and oat mixture, with 1：The step of being crushed after 3 ratio mixing water；
The step of adding oligosaccharide；
The step of maintaining more than 80 DEG C of temperature sterilize for the first time；
Be inoculated with mixed lactic bacteria culture fluid and more than 80 DEG C at a temperature of ferment 6 it is small when more than the step of；
The step of maintaining more than 85 DEG C of temperature to carry out second of sterilization；
The step of by centrifuging separation and fermentation thing；
To separated fermentate addition save agent from damage the step of and
More than 85 DEG C of temperature is maintained to carry out the step of third time is sterilized.
In the manufacture method of the Synbiotics tunning of the present invention, above-mentioned mixed lactic bacteria culture fluid is respectively with identical
Volume ratio mixing bifidobacterium longum SPM 1205 (accession number KCTC10630BP), lactobacillus plantarum KCTC 1048 and pentose piece ball
Bacterium CBT Sl4 nutrient solutions form.
In the manufacture method of the Synbiotics tunning of the present invention, 5 are included in the every 1ml of above-mentioned mixed lactic bacteria culture fluid
The lactic acid bacteria of more than × 108 quantity.
The present invention Synbiotics tunning manufacture method in, in above-mentioned the step of being crushed, by with
The grinding stone device of the low speed rotation of 30RPM is crushed.
In the manufacture method of the Synbiotics tunning of the present invention, above-mentioned oligosaccharide is relative to the mixed of above-mentioned beans and oat
100 parts by weight of compound add 1~20 parts by weight.
In the manufacture method of the Synbiotics tunning of the present invention, above-mentioned to save agent from damage be grapefruit seed extracting solution.
In addition, the present invention provides through Synbiotics fermentation production made from the manufacture method of the Synbiotics tunning of the present invention
Thing.
The Synbiotics tunning of the present invention includes the beta glucan particle of below 0.2um.
The Synbiotics tunning of the present invention can make an addition to various food, above-mentioned food can be meat, sausage, bread,
Chocolate, carbohydrate, snack class, Biscuits, Piza, instant noodles, other noodles, chewing gum class, include the breast including ice cream class
Product, soup, beverage, tea, healthy beverage, alcoholic beverage, powder formulation and compound vitamin but unrestricted.
Invention effect
The manufacture method of the Synbiotics tunning of the present invention, the nutrient source as probiotics use mixing beans and parkin
The prebiotics (prebiotics) crushed using low speed grinding stone, therefore obtained Synbiotics tunning includes water solubility
The size of colloid form is less than 0.2um and the beta glucan particle stablized to temperature and acid, remains strong so as to can be developed into and have
Health, strengthen immunity, the healthy food of pre- anti-cancer and assistant treating cancer effect.
Summary of drawings
Fig. 1 is the beta glucan testing result schematic diagram to the Synbiotics tunning of the present invention.
Embodiment
In the following, by embodiment, the present invention will be described in more detail.But the present invention is from the limit of following embodiments
System.
<Embodiment 1>The manufacture of Synbiotics tunning
First, with 1：2 ratio mixing beans and oat, after removing other foreign matters such as stone and soil with stone picker, are used
Water cleans soil and dust etc..
The beans of above-mentioned cleaning and oat, oat and the immersion of 150~250 parts by weight are mixed relative to the beans of 100 parts by weight
In water, after when maintenance 18 is small at 10 DEG C, the temperature of water is improved to 90 DEG C and maintained 30 minutes.
Soaking water is removed from the beans and oat mixture of above-mentioned immersion, relative to the beans and oat mixture 100 of immersion
Parts by weight, with 1：After 3 ratio mixing water, by being crushed with the grinding stone device of the low speed rotation of below 30RPM.
Beans and oat mixture to crushing add oligosaccharide and maintain more than 80 DEG C of temperature to carry out first time sterilization, into
Row fermentation make its in every 1ml comprising more than 5 × 108 lactic acid bacteria after, the mixed lactic bacteria culture fluid of inoculation fermentation and
More than when fermentation 6 is small at a temperature of more than 80 DEG C.
Inoculation is with same volume than mixing bifidobacterium longum SPM 1205, lactobacillus plantarum KCTC 1048 and pentose piece ball
Mixing lactic acid bacteria that bacterium CBT Sl4 are formed simultaneously ferments, to manufacture mixed lactic bacteria culture fluid.Bifidobacterium longum
(Bifidobacterium longum) SPM1205 is the novel microbial that identification is separated from Korean, has and promotes gut function
And the characteristics of reinforced immunological function, educate university's sustenance (accession number by three：KCTC 10630BP), August is applied on the 12nd within 2004,
On April 7th, 2006 registers (number of registration 05680).
Maintain after carrying out second sterilizing while 85 DEG C, by centrifuging fermentate.To the fermentate isolated
Addition saves agent from damage and maintains more than 80 DEG C progress third time sterilizations, so as to produce Synbiotics tunning.
<Embodiment 2>The manufacture of Synbiotics tunning
In addition to only oat is used, Synbiotics tunning is manufactured by the method identical with above-described embodiment 1.
<Experimental example>Detect beta glucan
To embodiment 1 and embodiment 2 manufacture tunning measurement beta glucan content and protein content and by its
As a result represent in table 1 below.Quantitative analysis utilizes the Enzymatic Yeast Beta- of Megazyme companies (Ireland)
Glucan Kit Assay Kit are analyzed according to protocol.
[table 1]
| Final product | pH | Proportion | Viscosity (20 DEG C) | Beta glucan | Protein |
| Embodiment 1 | 4.3 | 1.018 | 1,620cPs | 1,200mg/L | 3.661 |
| Embodiment 2 | 4.27 | 1.012 | 1,650cPs | 1,500mg/L | 2.939 |
<Experimental example>Detect beta glucan
The result for the Synbiotics tunning that above-described embodiment 1 manufactures will be filtered through using the filter in 0.2 μm of aperture
It is shown in Fig. 1.
In Fig. 1, A is D-Glucose standard solution, and B is distilled water, and C is extracting solution, and D is the upper liquid centrifuged, E
To filter the testing result after extracting solution using the filter of 0.45um sizes, F is to be filtered using the filter of 0.2um sizes
Testing result after extracting solution, G are the testing result to the extracting solution extracted after sterilizing.
In Fig. 1, all shown in C, D, E, F and G identical with A as a result, it may thus be appreciated that in the embodiment of the present invention
The Synbiotics tunning of manufacture is the particle identical with D-Glucose standard solution.
Industrial applicibility
The manufacture method of the Synbiotics tunning of the present invention, the nutrient source as probiotics use mixing beans and parkin
The prebiotics (prebiotics) crushed using low speed grinding stone, therefore obtained Synbiotics tunning includes water solubility
The size of colloid form is less than 0.2um and the beta glucan particle stablized to temperature and acid, remains strong so as to can be developed into and have
Health, strengthen immunity, the healthy food of pre- anti-cancer and assistant treating cancer effect.
Claims (9)
1. a kind of manufacture method of Synbiotics tunning, including：The step of cleaning beans and oat；
The beans of above-mentioned cleaning and oat, mix the oat of 150~250 parts by weight relative to the beans of 100 parts by weight and are immersed in water
In after, at 10~25 DEG C maintain 10 it is small when more than the step of；
By the temperature of water improve to more than 80 DEG C and maintain 30 minutes~1 it is small when the step of；
Relative to 100 parts by weight of beans and oat mixture, with 1：The step of being crushed after 3 ratio mixing water；
The step of adding oligosaccharide；
The step of maintaining more than 80 DEG C of temperature sterilize for the first time；
Be inoculated with mixed lactic bacteria culture fluid and more than 80 DEG C at a temperature of ferment 6 it is small when more than the step of；
The step of maintaining more than 85 DEG C of temperature to carry out second of sterilization；
The step of by centrifuging separation and fermentation thing；
To separated fermentate addition save agent from damage the step of and
More than 85 DEG C of temperature is maintained to carry out the step of third time is sterilized.
2. the manufacture method of Synbiotics tunning according to claim 1, it is characterised in that：Above-mentioned mixing lactic acid bacteria training
Nutrient solution is respectively with identical volume ratio mixing bifidobacterium longum SPM 1205 (accession number KCTC10630BP), lactobacillus plantarum
What KCTC 1048 and Pediococcus pentosaceus CBT Sl4 nutrient solutions formed.
3. the manufacture method of Synbiotics tunning according to claim 1, it is characterised in that：Above-mentioned mixing lactic acid bacteria training
The lactic acid bacteria of more than 5 × 108 quantity is included in the every 1ml of nutrient solution.
4. the manufacture method of Synbiotics tunning according to claim 1, it is characterised in that：Crushed above-mentioned
In step, by being crushed with the grinding stone device of the low speed rotation of 30RPM.
5. the manufacture method of Synbiotics tunning according to claim 1, it is characterised in that：Above-mentioned oligosaccharide relative to
Above-mentioned beans and 100 parts by weight of the mixture of oat add 1~20 parts by weight.
6. the manufacture method of Synbiotics tunning according to claim 1, it is characterised in that：It is above-mentioned that to save agent from damage be grape
Shaddock seed extracting solution.
7. a kind of manufacture method of any one of them Synbiotics tunning by according to claim 1 to 6 is fabricated
Synbiotics tunning.
8. Synbiotics tunning according to claim 7, it is characterised in that：Above-mentioned Synbiotics tunning includes
The beta glucan particle of below 0.2um.
9. Synbiotics tunning according to claim 7, it is characterised in that：Above-mentioned Synbiotics tunning is to include meat
Class, sausage, bread, chocolate, carbohydrate, snack class, Biscuits, Piza, instant noodles, other noodles, chewing gum class, ice cream class
Dairy products, soup, beverage, tea, healthy beverage, alcoholic beverage, powder formulation and compound vitamin inside.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR20150093978 | 2015-07-01 | ||
| KR1020150093978 | 2015-07-01 | ||
| PCT/KR2016/007146 WO2017003263A1 (en) | 2015-07-01 | 2016-07-01 | Method for preparing products of synbiotic fermentation, and products of synbiotic fermentation prepared thereby |
Publications (1)
| Publication Number | Publication Date |
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| CN107920575A true CN107920575A (en) | 2018-04-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201680050533.8A Pending CN107920575A (en) | 2015-07-01 | 2016-07-01 | A kind of manufacture method of Synbiotics tunning and the Synbiotics tunning thus manufactured |
Country Status (3)
| Country | Link |
|---|---|
| KR (1) | KR101805863B1 (en) |
| CN (1) | CN107920575A (en) |
| WO (1) | WO2017003263A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114304513A (en) * | 2022-02-11 | 2022-04-12 | 仙婷(广州)科技研发有限公司 | Oral composite strain fermentation product and application thereof |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102260320B1 (en) | 2017-07-31 | 2021-06-04 | (주)진바이오텍 | Synbiotic composition comprising lactulose and probiotic |
| KR20200035615A (en) | 2018-09-27 | 2020-04-06 | 충북대학교 산학협력단 | Synbiotic composition Using nonsoluble grains and Method for Manufacturing the Same |
| KR102098808B1 (en) | 2018-12-24 | 2020-04-16 | 비타민하우스(주) | Composition of synbiotics for suppressing constipation and diarrhea induction comprising Lactobacillus plantarum VH13 and Undaria pinnatifida Sporophyll hot-water extract |
| KR102227703B1 (en) * | 2019-04-01 | 2021-03-15 | 오창원 | A method for producing a plant lactic acid bacterium stock solution and a method for producing a botanical lactic acid bacterium stock solution prepared by the method for producing a plant lactic acid bacterium and a method for producing a plant lactic |
| KR20210063146A (en) | 2019-11-22 | 2021-06-01 | 충북대학교 산학협력단 | Composition for antioxidant activation and anti-inflammatory comprising mixed grains and lactic acid bacteria |
| KR102559441B1 (en) * | 2021-01-29 | 2023-07-24 | 이상만 | Method for producing beta-glucan-containing protein chocolate balls |
| WO2024071954A1 (en) * | 2022-09-29 | 2024-04-04 | 클립스비엔씨 주식회사 | Adjuvant composition comprising oat-derived β-glucan as active ingredient |
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| MX2010008879A (en) * | 2008-02-14 | 2010-10-20 | Barley & Oats Co Ltd | Method for producing fermented product using natural material, and food or medicine containing fermented product made from same. |
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| KR101458556B1 (en) * | 2012-01-20 | 2014-11-05 | 부산대학교 산학협력단 | Fermentation products of probiotic mixed cultures and functional health foods, medicinal composition including the same |
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2016
- 2016-07-01 KR KR1020160083539A patent/KR101805863B1/en active IP Right Grant
- 2016-07-01 WO PCT/KR2016/007146 patent/WO2017003263A1/en active Application Filing
- 2016-07-01 CN CN201680050533.8A patent/CN107920575A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101018491A (en) * | 2004-08-27 | 2007-08-15 | 普拉克生化公司 | Composition for inactivating yeasts or molds in soft drinks |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114304513A (en) * | 2022-02-11 | 2022-04-12 | 仙婷(广州)科技研发有限公司 | Oral composite strain fermentation product and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2017003263A1 (en) | 2017-01-05 |
| KR20170004897A (en) | 2017-01-11 |
| KR101805863B1 (en) | 2017-12-07 |
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