CN104814502B - A kind of probiotics cereal beverage and pulvis and preparation method thereof - Google Patents

A kind of probiotics cereal beverage and pulvis and preparation method thereof Download PDF

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Publication number
CN104814502B
CN104814502B CN201510284945.5A CN201510284945A CN104814502B CN 104814502 B CN104814502 B CN 104814502B CN 201510284945 A CN201510284945 A CN 201510284945A CN 104814502 B CN104814502 B CN 104814502B
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China
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probiotics
cfu
cereal
cereal beverage
beverage
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CN201510284945.5A
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CN104814502A (en
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路福平
王海宽
张楠
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天津科技大学
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A23B - A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38Other non-alcoholic beverages
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A23B - A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/70Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • A23L2/84Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23YINDEXING SCHEME RELATING TO LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23Y2220/00Lactobacillus
    • A23Y2220/67Plantarum
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23YINDEXING SCHEME RELATING TO LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23Y2300/00Bifidobacterium

Abstract

The present invention provides a kind of probiotics cereal beverage and pulvis and preparation method thereof, the probiotics cereal beverage is that the raw material composition of active constituent in every 1000ml water is:Cereal 8 12%, 40 50u/ml of amylase, 100 120u/ml of protease are inoculated at least one probiotics, and the bacterium amount that connects of each probiotics is 106‑107CFU/ml, it is cleaned and crush after cereal etc. is mixed according to a certain percentage, it sterilizes after enzymolysis, probiotics is accessed after sterilizing to ferment, product after fermentation is centrifuged, supernatant is through sterile allotment, canned product to obtain the final product, and probiotics powder agent can be made through compounding, vacuum freeze drying by centrifuging the deposits such as obtained probiotics, and total viable count is up to 10 in gained probiotics cereal beverage8‑109CFU/ml, total viable count is up to 10 in probiotics powder agent10‑1011CFU/g。

Description

A kind of probiotics cereal beverage and pulvis and preparation method thereof
Technical field
The present invention relates to microbe application fields, are related to a kind of probiotics cereal beverage and bacterium powder and preparation method thereof.
Background technology
With economic development, the improvement of living, people tend to eat more animal foods, are taken the photograph in resident's dietary structure The animal foods growth ratios such as the meat, egg, the newborn class that enter are excessively high, and the intake of the crops such as grain cereal gradually decreases, this The dietary fiber that diet structure provides is too low, and energy and fat are excessively high, cause rich people's disease and substantially rise to.With scientific and technological level Progress, the use of antibiotic, hormone, various chemicals make one internal metabolic system, enzyme system, immune system and all receive Different degrees of influence, the microecological balance in human body is seriously damaged, so as to cause modern many places in sub-health state.
Carbohydrate content is generally 75%-80%, protein content 8%-10%, fat content in cereal preparation 1% or so, also containing minerals, B family vitamin and dietary fiber etc..Adhere to based on cereal, can effectively keep China's diet Good tradition, the drawbacks of avoiding high-energy, high in fat and low carbohydrate diet.
Probiotics is a kind of by improving intestine microenvironment so as to the microorganism to host's generation beneficial effect.It can Inhibit growth of pathogenic bacteria by participating in intestinal microecology balance, avoid and drug resistance that antibiotic generates and double is used for a long time Infection, therefore, probiotic products receive the concern of people in recent years.
The deep processing of the crops such as cereal is developed into probiotics cereal foods by lactobacillus-fermented, can not only retain or increase The nutritional ingredient beneficial to health in these crops, and mouthfeel can be improved, it eats and is more convenient, absorption is easier.Shen Number it please disclose a kind of synchronous solid fermentation and enzymolysis prepares the production of fermented bean dregs for 201210273454.7 patent of invention Technique mainly digests effect synchronous synergetic with microbiological anaerobic solid state fermentation using dregs of beans.Although to the uniformity in the technique There is certain requirement, but since in solid state fermentation, there are gradients for nutrient concentrations, ferment uneven so that the life of thalline There are non-uniform problems for secretion long, to the absorption of nutrients and metabolite etc..Moreover, solid state fermentation production mechanization journey It spends relatively low, lacks online sensor apparatus, process control is more difficult.
Invention content
The technical problems to be solved by the invention are to provide a kind of probiotics cereal beverage and probiotics powder agent.
Another technical problem to be solved by this invention is to provide the preparation side of above-mentioned probiotics cereal beverage and pulvis Method.
In order to solve the above technical problems, the technical scheme is that:
A kind of probiotics cereal beverage, often the raw material composition of active constituent is in 1000ml water:Cereal 8-12%, amylase 40-50u/ml, protease 100-120u/ml are inoculated at least one probiotics, and each probiotics connects bacterium amount 106-107CFU/ ml。
Preferably, above-mentioned probiotics cereal beverage, water content 90%.
Preferably, above-mentioned probiotics cereal beverage, the cereal is oat, wheat, rice, mustard wheat, brown rice, sorghum, small Rice, corn, glutinous rice, black rice, red bean, mung bean, the one of which of soybean and black soya bean or arbitrary combination.
Preferably, above-mentioned probiotics cereal beverage, the cereal are made of oat, soybean and black soya bean, count in parts by weight 10-15 parts of oat, 10-15 parts of soybean, 10-15 parts of black soya bean.
Preferably, above-mentioned probiotics cereal beverage, the cereal are made of oat, soybean and black soya bean, count in parts by weight 10.5 parts of oat, 14 parts of soybean, 10.5 parts of black soya bean.
Preferably, above-mentioned probiotics cereal beverage, the probiotics are made of lactobacillus plantarum and animal bifidobacteria, In, lactobacillus plantarum connects bacterium amount and is:106-107CFU/ml, animal bifidobacteria connect bacterium amount and are:106-107CFU/ml。
Preferably, above-mentioned probiotics cereal beverage, the probiotics are made of lactobacillus plantarum and animal bifidobacteria, Middle lactobacillus plantarum connects bacterium amount 5-6 × 106CFU/ml, animal bifidobacteria connect bacterium amount 5-6 × 106CFU/ml。
A kind of preparation method of probiotics cereal beverage, is as follows:
(1) it is crushed after cereal is cleaned through beater;
(2) it is separately added into amylase after being heated in feed liquid after being pulverized and protease is digested, wherein, amylase adds Dosage is 40-50u/ml, enzymatic hydrolysis condition is 60 DEG C -65 DEG C, 60-80min, protease additive amount 100-120u/ml, enzymatic hydrolysis condition For 55 DEG C -60 DEG C, 100-120min, after enzymolysis, the control of DE values is in 30-50%, and alpha-amido Control of Nitrogen Content is in 1.0-2.0g/ 100ml;
(3) feed liquid after enzymolysis is sterilized and enzyme deactivation, wherein, the sterilising conditions are 115 DEG C of sterilizing 30min, together When enzyme deactivation;
(4) after feed liquid cooling, probiotics is accessed in matrix after sterilization, is fermented, wherein, each probiotics connects Bacterium amount is 106-107CFU/ml;
(5) after fermentation, tunning is centrifuged, centrifugal condition 3000-5000r/min, 30-60min are obtained Supernatant is probiotics cereal beverage;5%-15% is added in thalline obtained by centrifugation and insoluble precipitate (with centrifugation gained The weight percent of thalline and insoluble precipitate total weight) oligofructose as protective agent, -70~-50 DEG C of pre-freeze 1-5h Afterwards, vacuum freeze drying is carried out, probiotics powder agent is made in vacuum degree 1-8pa, vacuum freeze drying 18-22h.
Preferably, the preparation method of above-mentioned probiotics cereal beverage, each probiotics connects bacterium amount as 5- in the step (4) 6×106CFU/ml, incubation time 18h, 37 ± 1 DEG C of cultivation temperature.
Preferably, the preparation method of above-mentioned probiotics cereal beverage, centrifugation gained supernatant can be made into probiotics cereal drink Material, centrifugation gained precipitation can be made into probiotics powder agent, realize the maximization of raw material availability, and generate without waste liquid.
Preferably, it is reachable that the total viable count of probiotics cereal beverage is made in the preparation method of above-mentioned probiotics cereal beverage 108-109CFU/ml, total viable count is up to 10 in pulvis10-1011CFU/g。
Preferably, the preparation method of above-mentioned probiotics cereal beverage, the thalline and insoluble of centrifugation gained described in step (5) The oligofructose of sediment addition 10% is as protective agent;Sterile food flavor, stabilizer, pectin are added in the supernatant And virgin rubber, wherein, it is 0.05-0.1% by the volume percentage food flavor additive amount of supernatant;Stabilizer additive amount is CMC 0.15-0.4%, pectin 0.15-0.3%, xanthans 0.15-0.3%.
The beneficial effects of the invention are as follows:
Probiotics cereal beverage of the present invention had not only saved the nutritive value of the crops such as cereal, but also with probiotics The nutrition health-care functions of fermented product, it is unique in taste;The probiotics cereal beverage is prepared by specific process, for cereal The digestion of two kinds of most macromolecular nutritional ingredient starch of middle content and protein is target, is distinguished using amylase and protease Starch in cereal and protein are hydrolyzed into small molecule nutritional ingredient for being easy to probiotics and absorption of human body etc., and then pass through benefit Raw bacterium ferments to prepare probiotics cereal beverage and probiotics powder agent, while ensureing that fermentation substrate is uniform, it is easy to accomplish big The production of scale.
The preparation method of probiotics cereal beverage of the present invention is sought according to the human body of all ages and classes stage or different crowd Absorption characteristics, taste and particular/special requirement etc. are supported, the crops such as specific foodstuff grain is selected, cereal etc. is mixed according to a certain percentage Cleaned and crush after conjunction, through enzymolysis after sterilize, after sterilizing access probiotics ferment, by the product after fermentation carry out from The heart, supernatant through sterile allotment, canned up to product, and centrifuge the deposits such as obtained probiotics can be through compounding, vacuum Probiotics powder agent is made in freeze-drying.This collected bacterial sediment obtained of centrifugation and the plant fiber etc. of fermenting is insoluble Substance has very big market development potential by being processed into the technique rich in probiotics powder agent, and specific prominent effect is as follows:
(1) raw material range of choice is extensive, can be made full use of according to the trophic function feature of the crops such as various foodstuff grains Macromolecular substances in raw material are resolved into the small-molecule substance for being easier to absorption of human body by enzyme and the characteristic of probiotics, also more sharp It is grown in microorganism;
(2) general probiotics powder agent raw materials for production cost is higher, can generate a large amount of waste liquids in process of production, cause raw material Waste and environmental pollution;Using crops such as foodstuff grains as raw material, production cost is greatly reduced;Work is made by the present invention Skill, functional beverage is made in supernatant, and probiotics powder agent is then made in solid precipitation, realizes the maximization of raw material availability, And it is generated without waste liquid;
(3) in the probiotics cereal beverage obtained by this preparation method other than containing a large number of nutrients, contain The total viable count of probiotics is up to 108-109CFU/ml, the total viable count of probiotics is up to 10 in gained pulvis10-1011CFU/g。
Description of the drawings
Fig. 1:Cereal adding proportion is to the shadow of total viable count in gained probiotics cereal beverage after fermentation and probiotics powder agent It rings;
Fig. 2:Influence of the inoculum concentration to total viable count in gained probiotics cereal beverage after fermentation and probiotics powder agent;
Fig. 3:Influence of the incubation time to total viable count in gained probiotics cereal beverage after fermentation and probiotics powder agent.
Specific embodiment
Technical solution of the present invention is further described with reference to specific embodiment.
Embodiment 1
A kind of probiotics cereal beverage and pulvis, often the raw material composition of active constituent is in 1000ml water:Cereal 10% forms sediment Powder enzyme 45u/ml, protease 110u/ml are inoculated with probiotics:Lactobacillus plantarum (CGMCC1.6971, CGMCC:Chinese common micro- life Object culture presevation administrative center) and animal bifidobacteria (CGMCC 1.3003), wherein lactobacillus plantarum 6 × 106CFU/ml, it moves Object Bifidobacterium 6 × 106CFU/ml, the cereal are made of oat, soybean and black soya bean, in parts by weight count 10.5 parts of oat, 14 parts of soybean, 10.5 parts of black soya bean.
The preparation method of above-mentioned probiotics cereal beverage and pulvis, is as follows:
(1) it is crushed after cereal is cleaned through beater;
(2) it is separately added into amylase after being heated in feed liquid after being pulverized and protease is digested, wherein, amylase adds Dosage is 45u/ml, enzymatic hydrolysis condition is 63 DEG C, 70min, protease additive amount 110u/ml, enzymatic hydrolysis condition be 57 DEG C, 110min, After enzymolysis, the control of DE values is 32%, and alpha-amido Control of Nitrogen Content is in 1.2g/100ml;
(3) feed liquid after enzymolysis is sterilized and enzyme deactivation, wherein, the sterilising conditions are 115 DEG C of sterilizing 30min, together When enzyme deactivation;
(4) accessed after feed liquid cooling, in matrix after sterilization probiotics lactobacillus plantarum and animal bifidobacteria into Row fermentation, wherein, it is 6 × 10 that each probiotics, which connects bacterium amount,6CFU/ml, incubation time 18h, 37 ± 1 DEG C of cultivation temperature;
(5) after fermentation, tunning is centrifuged, centrifugal condition 4000r/min, 45min, obtaining supernatant is Probiotics cereal beverage;The oligofructose of gained thalline and insoluble precipitate addition 10% will be centrifuged as protective agent, -60 DEG C After pre-freeze 3h, vacuum freeze drying is carried out, probiotics powder agent is made in vacuum degree 5pa, vacuum freeze drying 20h.
Total viable count in gained probiotics cereal beverage is 7.35 × 108CFU/ml.Wherein, lactobacillus plantarum viable count 6.38×108CFU/ml, the reachable viable count of animal bifidobacteria is 9.70 × 107CFU/ml.Total viable bacteria in probiotics powder agent Number is 7.21 × 1010CFU/g.Wherein lactobacillus plantarum viable count 6.47 × 1010The viable count of CFU/g and animal bifidobacteria Reachable is 7.40 × 109CFU/g。
Embodiment 2
A kind of probiotics cereal beverage and pulvis, often the raw material composition of active constituent is in 1000ml water:Cereal 8% forms sediment Powder enzyme 50u/ml, protease 100u/ml are inoculated with probiotics:Lactobacillus plantarum (CGMCC1.3921) and animal bifidobacteria (CGMCC 1.1852), wherein lactobacillus plantarum 106CFU/ml, animal bifidobacteria 106CFU/ml, the cereal is by oat, big Beans and black soya bean composition, count 15 parts of oat, 10 parts of soybean, 15 parts of black soya bean in parts by weight.
The preparation method of above-mentioned probiotics cereal beverage and pulvis, is as follows:
(1) it is crushed after cereal is cleaned through beater;
(2) it is separately added into amylase after being heated in feed liquid after being pulverized and protease is digested, wherein, amylase adds Dosage is 50u/ml, enzymatic hydrolysis condition is 60 DEG C, 80min, protease additive amount 100u/ml, enzymatic hydrolysis condition be 60 DEG C, 100min, After enzymolysis, the control of DE values is 30%, and alpha-amido Control of Nitrogen Content is in 2.0g/100ml;
(3) feed liquid after enzymolysis is sterilized and enzyme deactivation, wherein, the sterilising conditions are 115 DEG C of sterilizing 30min, together When enzyme deactivation;
(4) accessed after feed liquid cooling, in matrix after sterilization probiotics lactobacillus plantarum and animal bifidobacteria into Row fermentation, wherein, it is 10 that each probiotics, which connects bacterium amount,6CFU/ml, incubation time 14h, 37 ± 1 DEG C of cultivation temperature;
(5) after fermentation, tunning is centrifuged, centrifugal condition 3000r/min, 60min, obtaining supernatant is Probiotics cereal beverage;The oligofructose of gained thalline and insoluble precipitate addition 5% will be centrifuged as protective agent, -70 DEG C After pre-freeze 1h, vacuum freeze drying is carried out, probiotics powder agent is made in vacuum degree 8pa, vacuum freeze drying 18h.
Total viable count in gained probiotics cereal beverage is 6.69 × 108CFU/ml.Wherein, lactobacillus plantarum viable count 6.10×108CFU/ml, the reachable viable count of animal bifidobacteria is 5.90 × 107CFU/ml.Total viable bacteria in probiotics powder agent Number is 6.20 × 1010CFU/g.Wherein lactobacillus plantarum viable count 5.61 × 1010The viable count of CFU/g and animal bifidobacteria Reachable is 5.50 × 109CFU/g。
Embodiment 3
A kind of probiotics cereal beverage and pulvis, often the raw material composition of active constituent is in 1000ml water:Cereal 12% forms sediment Powder enzyme 40u/ml, protease 120u/ml are inoculated with probiotics:Lactobacillus plantarum (CGMCC1.3915) and animal bifidobacteria (CGMCC 1.1852), wherein lactobacillus plantarum 107CFU/ml, animal bifidobacteria 107CFU/ml, the cereal is by oat, big Beans and black soya bean composition, count 12 parts of oat, 13 parts of soybean, 11 parts of black soya bean in parts by weight.
The preparation method of above-mentioned probiotics cereal beverage and pulvis, is as follows:
(1) it is crushed after cereal is cleaned through beater;
(2) it is separately added into amylase after being heated in feed liquid after being pulverized and protease is digested, wherein, amylase adds Dosage is 40u/ml, enzymatic hydrolysis condition is 65 DEG C, 60min, protease additive amount 120u/ml, enzymatic hydrolysis condition be 55 DEG C, 120min, After enzymolysis, the control of DE values is 50%, and alpha-amido Control of Nitrogen Content is in 1.0g/100ml;
(3) feed liquid after enzymolysis is sterilized and enzyme deactivation, wherein, the sterilising conditions are 115 DEG C of sterilizing 30min, together When enzyme deactivation;
(4) accessed after feed liquid cooling, in matrix after sterilization probiotics lactobacillus plantarum and animal bifidobacteria into Row fermentation, wherein, it is 10 that each probiotics, which connects bacterium amount,7CFU/ml, incubation time 26h, 37 ± 1 DEG C of cultivation temperature;
(5) after fermentation, tunning is centrifuged, centrifugal condition 5000r/min, 30min, obtaining supernatant is Probiotics cereal beverage;The oligofructose of gained thalline and insoluble precipitate addition 15% will be centrifuged as protective agent, -50 DEG C After pre-freeze 5h, vacuum freeze drying is carried out, probiotics powder agent is made in vacuum degree 1pa, vacuum freeze drying 22h.
Total viable count in gained probiotics cereal beverage is 6.53 × 108CFU/ml.Wherein, lactobacillus plantarum viable count 6.01×108CFU/ml, the reachable viable count of animal bifidobacteria is 4.20 × 107CFU/ml.Total viable bacteria in probiotics powder agent Number is 6.73 × 1010CFU/g.Wherein lactobacillus plantarum viable count 6.08 × 1010The viable count of CFU/g and animal bifidobacteria Reachable is 6.50 × 109CFU/g。
Embodiment 4
A kind of probiotics cereal beverage and pulvis, often the raw material composition of active constituent is in 1000ml water:Cereal 11% forms sediment Powder enzyme 47u/ml, protease 115u/ml are inoculated with probiotics:Lactobacillus plantarum (CGMCC1.3915) and animal bifidobacteria (CGMCC 1.2226), wherein lactobacillus plantarum 5 × 106CFU/ml, animal bifidobacteria 5 × 106CFU/ml, the cereal by The weight ratios such as wheat, rice, mustard wheat, brown rice, sorghum, millet, corn, glutinous rice, black rice, red bean and mung bean mixing composition.
The preparation method of above-mentioned probiotics cereal beverage and pulvis, is as follows:
(1) it is crushed after cereal is cleaned through beater;
(2) it is separately added into amylase after being heated in feed liquid after being pulverized and protease is digested, wherein, amylase adds Dosage is 47u/ml, enzymatic hydrolysis condition is 62 DEG C, 75min, protease additive amount 115u/ml, enzymatic hydrolysis condition be 58 DEG C, 115min, After enzymolysis, the control of DE values is 35%, and alpha-amido Control of Nitrogen Content is in 1.5g/100ml;
(3) feed liquid after enzymolysis is sterilized and enzyme deactivation, wherein, the sterilising conditions are 115 DEG C of sterilizing 30min, together When enzyme deactivation;
(4) accessed after feed liquid cooling, in matrix after sterilization probiotics lactobacillus plantarum and animal bifidobacteria into Row fermentation, wherein, it is 5 × 10 that each probiotics, which connects bacterium amount,6CFU/ml, incubation time 22h, 37 ± 1 DEG C of cultivation temperature;
(5) after fermentation, tunning is centrifuged, centrifugal condition 4000r/min, 45min, obtaining supernatant is Probiotics cereal beverage;The oligofructose of gained thalline and insoluble precipitate addition 15% will be centrifuged as protective agent, -60 DEG C After pre-freeze 4h, vacuum freeze drying is carried out, probiotics powder agent is made in vacuum degree 4pa, vacuum freeze drying 20h.
Total viable count in gained probiotics cereal beverage is 6.51 × 108CFU/ml.Wherein, lactobacillus plantarum viable count 6.02×108CFU/ml, the reachable viable count of animal bifidobacteria is 4.90 × 108CFU/ml.Total viable bacteria in probiotics powder agent Number is 6.35 × 1010CFU/g.Wherein lactobacillus plantarum viable count 5.87 × 1010The viable count of CFU/g and animal bifidobacteria Reachable is 4.80 × 109CFU/g。
In above-described embodiment 1-4, gained supernatant (probiotics cereal beverage) can add in sterile according to needs of production Food flavor, stabilizer, pectin and virgin rubber, wherein, by supernatant volume percentage food flavor additive amount be 0.05- 0.1%;Stabilizer additive amount is CMC 0.15-0.4%, pectin 0.15-0.3%, xanthans 0.15-0.3%.
Embodiment 5 screens example
Screen determining for 1 cereal materials of example proportioning
After raw material is handled as stated above, it is 90% to match water content in liquid state fermentation matrix, oat:Soybean: Black soya bean ratio is respectively (%):2:3:5;3:3:4;3:4:3;4:3:3;4:4:2;115 DEG C of sterilizing 30min.
Connect bacterium amount and condition of culture:Access lactobacillus plantarum (CGMCC 1.6971) and animal bifidobacteria (CGMCC simultaneously 1.3003), the inoculum concentration of two strains of lactic acid bacteria is 5 × 106CFU/ml, pH cultivate 20h naturally, 37 ± 1 DEG C of cultivation temperature.It surveys The total viable count of probiotics after fixed fermentation in gained probiotics cereal beverage and probiotics powder agent carries out.
The results are shown in Figure 1:Best culture formula is (%):Oat:Soybean:Black soya bean ratio is 3:4:3.Measure gained Total viable count in probiotics cereal beverage is 3.21 × 108CFU/ml.Wherein, lactobacillus plantarum viable count 2.72 × 108CFU/ Ml, the reachable viable count of animal bifidobacteria is 4.9 × 107CFU/ml.Total viable count in probiotics powder agent for 4.70 × 1010CFU/g.Wherein lactobacillus plantarum viable count 4.42 × 1010The viable count of CFU/g and animal bifidobacteria up to for 4.8 × 109CFU/g。
Screen determining for 2 inoculum concentration of example
It is inoculated with simultaneously by the different bacterium amounts that connects in cereal matches the oat optimized, soybean, black soya bean mixes liquid matrix Lactobacillus plantarum (CGMCC 1.6971) and animal bifidobacteria (CGMCC 1.3003) ferment.
Connect bacterium amount and condition of culture:The bacterium amount that connects of two strains of lactic acid bacteria is respectively:2×106CFU/ml;4×106CFU/ml; 6×106CFU/ml;8×106CFU/ml.PH cultivates 20h altogether naturally, 37 ± 1 DEG C of cultivation temperature, prebiotic to gained after fermentation The total viable count of probiotics in bacterium cereal beverage and probiotics powder agent is detected.
The results are shown in Figure 2:The best bacterium amount that connects is 6 × 106CFU/ml is measured total in gained probiotics cereal beverage Viable count is 5.61 × 108CFU/ml.Wherein, lactobacillus plantarum viable count 4.92 × 108CFU/ml, the work of animal bifidobacteria Reachable bacterium number is 6.9 × 107CFU/ml.Total viable count in probiotics powder agent is 7.1 × 1010CFU/g.Wherein lactobacillus plantarum Viable count is 6.70 × 1010CFU/g, animal bifidobacteria viable count are 4.14 × 109CFU/g。
Screen determining for 3 incubation time of example
The oat that has optimized in cereal proportioning, soybean in black soya bean liquid state fermentation matrix while access inoculated plant breast bar (CGMCC 1.6971) and animal bifidobacteria (CGMCC 1.3003).
Connect bacterium amount and incubation time:The bacterium amount that connects of two strains of lactic acid bacteria is 6 × 10 respectively6CFU/ml, pH are naturally, culture temperature 37 ± 1 DEG C are spent, respectively fermentation 14,18,22,26h, to prebiotic in gained probiotics cereal beverage and probiotics powder agent after fermentation Bacterium viable count is detected.
The results are shown in Figure 3:Best incubation time is 18h.Measure total viable count in gained probiotics cereal beverage It is 7.85 × 108CFU/ml.Wherein, lactobacillus plantarum viable count 7.02 × 108The viable count of CFU/ml and animal bifidobacteria can Up to being 8.3 × 107CFU/ml.Total viable count in gained probiotics powder agent is 7.67 × 1010CFU/g.Wherein lactobacillus plantarum Viable count is 6.81 × 1010CFU/g, animal bifidobacteria viable count are 8.61 × 109CFU/g。
Embodiment 6 measures test example
Measure test example 1
It is total dietary fiber in probiotics cereal beverage before and after single bacterium, mixed bacterium enzymatic hydrolysis and fermentation, soluble dietary fiber, insoluble Dietary fiber, beta glucan, isoflavones and amino-acid nitrogen content measure
Using AACC32-07 and AOAC995.16 methods to before single bacterium or mixed bacterium enzymatic hydrolysis and fermentation and fermentation after gained probiotics paddy Total dietary fiber, soluble dietary fiber, insoluble dietary fiber content and beta glucan content in object beverage are surveyed Surely it the results are shown in Table 1.Using GB/T 23788-2009 methods, to gained probiotics paddy before single bacterium or mixed bacterium enzymatic hydrolysis and fermentation and after fermentation Isoflavone content in object beverage is measured, and the results are shown in Table 2.Using GB/T12143.2-89, single bacterium or mixed bacterium are measured The content of amino nitrogen before enzymatic hydrolysis and fermentation and after fermentation in gained probiotics cereal beverage, the results are shown in Table 3.Wherein, as weight Content is not much different in gained probiotics cereal beverage after wanting the beta glucan content of prebiotics before enzymolysis and fermenting, and slightly drops It is low.Isoflavones exists in the form of two kinds of glucosides and aglycon.In probiotics cereal beverage, it is easier to the glycosides being absorbed by the body First content increases.Under the action of enzyme and probiotics, amino nitrogen level increased protein.
Measure test example 2
Total dietary fiber, soluble dietary fiber, insoluble diet in probiotics powder agent before and after single bacterium, mixed bacterium enzymatic hydrolysis and fermentation Fiber, beta glucan, isoflavones and amino-acid nitrogen content measure
Using AACC32-07 and AOAC995.16 methods to total meals before enzymatic hydrolysis and fermentation and after fermentation in gained probiotics powder agent Food fiber, soluble dietary fiber, insoluble dietary fiber content and beta glucan content are measured and the results are shown in Table 4.Profit With GB/T 23788-2009 methods, the isoflavones before single bacterium or mixed fungus fermentation and after fermentation in gained probiotics powder agent is contained Amount is measured, and the results are shown in Table 5.Using GB/T12143.2-89, gained is prebiotic before measure single bacterium or mixed fungus fermentation and after fermentation The content of amino nitrogen in bacteria powder, the results are shown in Table 6.It is wherein, more as the reduction of the beta glucan content of important prebiotics, Mainly since in the gained precipitation after centrifugation or filtering, containing more insoluble diedairy fiber, soluble dietary fiber contains Amount is seldom.In probiotics powder agent, it is easier to which the Aglycones content being absorbed by the body increases.Protein is in the effect of enzyme and probiotics Under, amino nitrogen level increased.
Simultaneously containing high activity in probiotics cereal beverage obtained by specific example described in invention and probiotics powder agent Two kinds of probiotics.Wherein in probiotics cereal beverage, lactobacillus plantarum viable count is no less than 6.93 × 108CFU/ml, animal The viable count of Bifidobacterium is no less than 8.10 × 107CFU/ml;In probiotics powder agent, lactobacillus plantarum viable count is no less than 6.03×1010CFU/g, the viable count of animal bifidobacteria are no less than 5.65 × 109CFU/g.It is removed in product of the present invention containing rich Outside rich nutriment, also containing a large amount of probiotics.Lactobacillus plantarum mainly acts on the small intestine of human body, animal bifidobacteria master The large intestine of human body is acted on, so as to preferably improve intestine microenvironment, and then promote health.
Dietary fiber and β-Portugal gather before 1 single bacterium of table or mixed bacterium enzymatic hydrolysis and fermentation and after fermentation in gained probiotics cereal beverage The changes of contents of sugar
Glucosides and aglycon contain quantitative change in gained probiotics cereal beverage before 2 single bacterium of table or mixed bacterium enzymatic hydrolysis and fermentation and after fermentation Change
Before 3 single bacterium of table or mixed bacterium enzymatic hydrolysis and fermentation and fermentation after gained probiotics cereal beverage in amino nitrogen changes of contents
Dietary fiber and beta glucan in gained probiotics powder agent before 4 single bacterium of table or mixed bacterium enzymatic hydrolysis and fermentation and after fermentation Changes of contents
Before 5 single bacterium of table or mixed bacterium enzymatic hydrolysis and fermentation and fermentation after gained probiotics powder agent in the changes of contents of glucosides and aglycon
Before 6 single bacterium of table or mixed bacterium enzymatic hydrolysis and fermentation and fermentation after gained probiotics powder agent in amino nitrogen changes of contents
It in summary it can be seen:
1st, total viable count is higher than without digesting in gained probiotics cereal beverage and probiotics powder agent after enzymolysis process The viable count in sample obtained by journey.And by contained in the probiotics cereal beverage obtained by enzymolysis and two processes of mixed fungus fermentation The content of the nutriments such as some beta glucans and amino nitrogen is above obtained by only enzymolysis or the single process of mixed fungus fermentation Beverage.
2nd, freeze drying protectant can make the active material damage of biological products in freeze-drying process or after freeze-drying process, So as to improve survival rate and preservation of survival rate and freeze-dried products of the bioactive substance in freeze-drying process during preservation Phase.Screening the condition of vacuum freeze drying described in example is obtained by optimization, and 10% oligofructose of addition is protective agent, institute Total viable count highest, storage life longest in the probiotics powder agent obtained.
The above-mentioned detailed description carried out with reference to embodiment to a kind of probiotics cereal beverage and preparation method thereof, is explanation Property rather than it is limited, several embodiments can be enumerated according to limited range, therefore of the invention overall not departing from Change and modification under design, should belong within protection scope of the present invention.

Claims (7)

1. a kind of probiotics cereal beverage, it is characterised in that:The raw material composition of active constituent is in per 1000ml water:Cereal 8- 12%, amylase 40-50u/ml, protease 100-120u/ml, inoculating two kinds probiotics, and each probiotics 106-107CFU/ Ml, the cereal are made of oat, soybean and black soya bean, count 10-15 parts of oat, 10-15 parts of soybean, black soya bean 10- in parts by weight 15 parts, the probiotics is made of lactobacillus plantarum and animal bifidobacteria, wherein connecing bacterium amount and being:Lactobacillus plantarum 106- 107CFU/ml, animal bifidobacteria 106-107CFU/ml。
2. probiotics cereal beverage according to claim 1, it is characterised in that:Water content is 90%.
3. probiotics cereal beverage according to claim 1, it is characterised in that:The cereal is by oat, soybean and black soya bean Composition counts 10.5 parts of oat, 14 parts of soybean, 10.5 parts of black soya bean in parts by weight.
4. probiotics cereal beverage according to claim 1, it is characterised in that:The probiotics is by lactobacillus plantarum and moves Object Bifidobacterium forms, wherein connecing bacterium amount and being:Lactobacillus plantarum 5-6 × 106CFU/ml, animal bifidobacteria 5-6 × 106CFU/ ml。
5. the preparation method of probiotics cereal beverage described in claim 1, it is characterised in that:It is as follows:
(1) it is crushed after cereal is cleaned through beater;
(2) it is separately added into amylase after being heated in feed liquid after being pulverized and protease is digested, wherein, amylase additive amount It is 60 DEG C -65 DEG C for 40-50u/ml, enzymatic hydrolysis condition, 60-80min, protease additive amount 100-120u/ml, enzymatic hydrolysis condition 55 DEG C -60 DEG C, 100-120min, after enzymolysis, the control of DE values is in 30-50%, and alpha-amido Control of Nitrogen Content is in 1.0-2.0g/100ml;
(3) feed liquid after enzymolysis is sterilized and enzyme deactivation, wherein, the sterilising conditions are that 115 DEG C of sterilizing 30min go out simultaneously Enzyme;
(4) after feed liquid cooling, probiotics is accessed in matrix after sterilization, is fermented, wherein, each probiotics connects bacterium amount It is 106-107CFU/ml;
(5) after fermentation, tunning is centrifuged, centrifugal condition 3000-5000r/min, 30-60min obtain supernatant Liquid is probiotics cereal beverage;The oligofructose of gained thalline and insoluble precipitate addition 5%-15% will be centrifuged as protection Agent after -70~-50 DEG C of pre-freeze 1-5h, carries out vacuum freeze drying, vacuum degree 1-8pa, vacuum freeze drying 18-22h, system Into probiotics powder agent.
6. the preparation method of probiotics cereal beverage according to claim 5, it is characterised in that:It is every in the step (4) Kind probiotics connects bacterium amount as 5-6 × 106CFU/ml, incubation time 18h, 37 ± 1 DEG C of cultivation temperature.
7. the preparation method of probiotics cereal beverage according to claim 5, it is characterised in that:Described in step (5) from Thalline obtained by the heart and the oligofructose of insoluble precipitate addition 10% are as protective agent.
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