CN105942084A - Method for producing probiotic functional food through buckwheat fermentation - Google Patents
Method for producing probiotic functional food through buckwheat fermentation Download PDFInfo
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- CN105942084A CN105942084A CN201610283290.4A CN201610283290A CN105942084A CN 105942084 A CN105942084 A CN 105942084A CN 201610283290 A CN201610283290 A CN 201610283290A CN 105942084 A CN105942084 A CN 105942084A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/84—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
Abstract
The invention belongs to the field of functional foods, in particular relates to a method for producing a probiotic functional beverage and probiotic functional food through buckwheat fermentation and discloses a method for preparing a probiotic liquid state fermentation beverage by using a lot of probiotic mixed strains liquid state fermentation buckwheat and a method for preparing probiotic food by using a lot of probiotic mixed strains solid state fermentation buckwheat. The preparation process is simple; through lactobacillus fementation or non-fermentation, buckwheat, as cereal foods, has antioxidant, antihypertensive and hypoglycemic effects and preventive effects on cardiovascular and cerebrovascular diseases to some extent, and can coordinate intestinal tracts at the same time, thus the probiotic fermentation beverage and the solid state fermentation food can satisfy the needs of different consumer groups.
Description
Technical field:
The present invention relates to field of functional food, relate to the use of probiotics fermention Semen Fagopyri Esculenti and produce the side of functional food
Method.
Background technology:
Diabetes are incretion metabolism diseases the most common and the most occurred frequently.China's diabetes at present
People's quantity ranks first in the world, and making a definite diagnosis diabetes patient has nearly 100,000,000.Diabetes have a strong impact on patient daily life,
Long-term health, and make quality of life decline.At present, although the probiotic bacteria functional product on market is the abundantest,
But the product category being aimed at useful diabetes patient is little.This patent is intended to production is of value to diabetes patient
Edible functional drinks and food, and provide Research Thinking for Related product.
Probiotic bacteria is can to enter in digestive tract the sour environment being resistant in stomach and intestinal with viable bacteria form
Cholate and field planting is got off thus to health play beneficial effect active beneficial microorganism general name.Its benefit
Raw effect mainly by directly or indirectly adjusting the composition of host intestine microorganism, activates host's endogenous
Micropopulation or immune activity realize.Confirm that probiotic bacteria can effectively be treated and alleviate just at present
The diseases such as secret, diarrhoea, inflammatory bowel.Probiotic products enjoys consumer to pay close attention to the most in recent years.
Semen Fagopyri Esculenti is the crop of medicine-food two-purpose in nature, is the edible staple food enjoying diabetes patient to favor, has four
Individual kind is respectively sweet buckwheat, Radix Et Rhizoma Fagopyri Tatarici, wing buckwheat and rice buckwheat, based on sweet buckwheat and Radix Et Rhizoma Fagopyri Tatarici.Buckwheat protein contains
Abundant lysine composition, the trace element such as its ferrum, manganese, zinc abundanter than general corn and also containing abundant meals
Food fiber, so Semen Fagopyri Esculenti has good nutrition health-care functions.Modern pharmacological research shows that Semen Fagopyri Esculenti has blood sugar lowering fall
The effect of fat, its functional materials is Flavonoid substances and polyphenol, can effectively suppress amylase and α-glucoside
The activity of enzyme, but also insulin sensitivity can be improved, alleviate insulin resistant, protect beta Cell of islet, be
Natural plants insulin.The absorption of fat, peroxide activator body multiplication agent activated form receptor can be reduced simultaneously
Activity, improve lipid metabolism and reduce blood fat, can be used for treating diabetes and hyperlipemia.In Semen Fagopyri Esculenti possibly together with
Nicotinic acid composition, it is possible to promote the metabolism of body, strengthens function of detoxification, also has expansion thin vessels and reduction
The effect of Blood Cholesterol.
It is processed functional drinks and the food developed by probiotics fermention Semen Fagopyri Esculenti, battalion can not only be retained
Support, improve local flavor, moreover it is possible to increase functional components, absorb and be easier to.
The Chinese invention patent of application number 201310742433.X discloses a kind of mixing lactic acid bacteria fermentation and prepares buckwheat
The method of wheat beverage, step is as follows: weigh Fagopyrum esculentum Moench, adds clear water, anhydrous calcium chloride, α-amylase,
First liquefaction adds saccharifying enzyme saccharifying, then filters to obtain Semen Fagopyri Esculenti saccharified liquid, and Semen Fagopyri Esculenti saccharified liquid is accessed plant breast bar
Bacterium lyophilized powder, Lactobacillus bulgaricus lyophilized powder, streptococcus thermophilus lyophilized powder, anaerobism under the conditions of 34 DEG C-38 DEG C
Ferment 15-18 hour, then fermentation liquor allotment, filtration, fill, sterilizing, thus obtaining the product finished product.The buckwheat of the present invention
Wheat beverage effectively overcomes Semen Fagopyri Esculenti coarse mouthfeel, stodgy shortcoming, is a kind of nutritive value and health care valency
It is worth higher beverage, the also deep processing for Semen Fagopyri Esculenti and opens new way.
The Chinese invention patent of Application No. 201510604991.9 discloses the preparation of a kind of Radix Et Rhizoma Fagopyri Tatarici fermented beverage
Method, Radix Et Rhizoma Fagopyri Tatarici decortication is shelled and is ground into Radix Et Rhizoma Fagopyri Tatarici powder, carrying out enzymolysis and be filled into after Radix Et Rhizoma Fagopyri Tatarici powder gelatinizing by the method
Saccharifying filtrate；Carrying out lactate fermentation after saccharifying filtrate sterilizing, fermentation liquid fine straining obtains fermenation raw liquid；Fermenation raw liquid
Modulation obtains Radix Et Rhizoma Fagopyri Tatarici fermented beverage.The preparation method of the Radix Et Rhizoma Fagopyri Tatarici fermented beverage that the present invention provides, is carried out Radix Et Rhizoma Fagopyri Tatarici powder
Extraction, can improve Radix Et Rhizoma Fagopyri Tatarici flavone and the dissolution rate of other Radix Et Rhizoma Fagopyri Tatarici inclusions by enzymolysis, saccharifying, and products obtained therefrom has
There is the pleasant ferment local-flavor of uniqueness.
But the buckwheat foods production and processing method provided in prior art is complicated, and nutrition content is low, this
Invention will select suitably combination bacterial strain mixed fungus fermentation, utilize the synergic fermentation effect between bacterial strain, can improve
Productivity, promote growth and breeding each other, the producing enzyme, produce acid and produce nutrient substance and substrate is divided of combination bacterial strain
Solve Utilization ability etc. and to be significantly better than single bacterium fermentation.Therefore, it can by liquid and two kinds of techniques pair of solid fermentation
Semen Fagopyri Esculenti is combined the mixed fungus fermentation of bacterial strain, produces Semen Fagopyri Esculenti probiotic bacteria functional food.
Summary of the invention:
It is an object of the invention to utilize multiple probiotic bacteria to mix bacterium and Semen Fagopyri Esculenti is carried out a kind of probiotic bacteria merit of fermentation acquisition
Can property food.The most all as a example by Lactobacillus plantarum and animal bifidobacteria two strain bacterium, carry out the concrete of the present invention
Introduce.
To achieve these goals, the present invention provides a kind of Semen Fagopyri Esculenti liquid fermentation beverage: a kind of prebiotic bacterium solution of Semen Fagopyri Esculenti
State fermented beverage, is as fermentation substrate using Fagopyrum esculentum Moench powder and grain dust serosity raw material, uses Lactobacillus plantarum and dynamic
Thing bacillus bifidus is after mixed culture liquid state fermentation, after emulsifying, adding stabilizer, acid adjustment, perfumery, homogenizing, sterilization
It is prepared.
Described Fagopyrum esculentum Moench powder and grain dust serosity are particularly as follows: add 5～10% (mass volume ratio) in every 1000ml water
Fagopyrum esculentum Moench powder, the grain dust of 0-5% (mass volume ratio), stir；
The preparation method of above-mentioned Semen Fagopyri Esculenti probiotic bacteria liquid fermentation functional drinks, specifically comprises the following steps that
(1) after buckwheat shelling, Semen Fagopyri Esculenti and corn being cleaned, pulverizing is crossed 100 mesh sieves and is obtained Fagopyrum esculentum Moench powder and grain dust respectively；
(2) preparation activation medium: every 100ml water adds 5g Fagopyrum esculentum Moench powder (Fagopyrum esculentum Moench powder in activation medium with
Fagopyrum esculentum Moench powder kind in fermentation medium is consistent)；Activation medium is carried out sterilizing, and condition is, 121 DEG C,
20min；
(3) preparation fermentation medium: interpolation 5-10g Fagopyrum esculentum Moench powder in every 100ml water, the grain dust of 0-5g, 121 DEG C
Sterilizing 20min；
(4) actication of culture, Lactobacillus plantarum and animal bifidobacteria glycerol pipe are inoculated in activation medium by 1% respectively
Activating, pH7.2-7.4, controlling temperature is 37 DEG C；Respectively after activation 20h, by connecing of 10% (v/v)
Bacterium amount activation medium of again transferring carries out re-activation, after reactivation 24h secondary seed solution；
(5) probiotics fermention: be 1 × 10 by connecing bacterium amount by Lactobacillus plantarum6-5×107CFU/mL is inoculated in fermentation training
Support in base, inoculate animal bifidobacteria, Lactobacillus plantarum and animal bifidobacteria simultaneously and connect bacterium amount volume ratio and be
1-10:1-10, mixed-strains liquid fermentation 4～40h, temperature is 37 DEG C, and pH maintains 7.2～7.4；
(6) fermentation ends after fermentation liquid through emulsifying, add stabilizer, acid adjustment, perfumery, homogenizing, sterilization after i.e. obtain buckwheat
Wheat probiotic bacteria liquid fermentation beverage；
Described activation and fermentation are quiescent culture；
Described Semen Fagopyri Esculenti is sweet cherry roots or Radix Et Rhizoma Fagopyri Tatarici；
Described corn is at least one in Herba bromi japonici, Semen Tritici aestivi, corn and soybean, Semen sojae atricolor；
Preferably, consisting of of fermentation medium: add 5g sweet cherry roots powder, the Semen sojae atricolor of 2g in every 100ml water
Powder；
Preferably, consisting of of fermentation medium: add 5g Radix Et Rhizoma Fagopyri Tatarici powder, the Semen sojae atricolor of 5g in every 100ml water
Powder；
Preferably, Lactobacillus plantarum and animal bifidobacteria inoculum concentration are 5 × 107CFU/mL；
Preferably, the mixed-strains liquid fermentation time is 28h；
Preferably, described Lactobacillus plantarum is Lactobacillus plantarum (Lactobacillus plantarum) TK9, this bacterium
Strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms in December in 2015 on the 17th
The heart, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica, postcode
100101, deposit number is CGMCC No.11891；
Preferably, described animal bifidobacteria is animal bifidobacteria (Bifidobacterium animalis) 937,
This bacterial strain is preserved in the common micro-life of China Committee for Culture Collection of Microorganisms on the 17th in December in 2015
Thing center, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica, postal
Compiling 100101, deposit number is CGMCC No.11892.
To achieve these goals, a kind of solid-state fermentation technology scheme that the present invention provides: a kind of Semen Fagopyri Esculenti probiotic bacteria
Solid fermentation food, is as fermentation substrate using Semen Fagopyri Esculenti, uses Lactobacillus plantarum and animal bifidobacteria through solid-state
It is prepared after mixed fungus fermentation；
Described Semen Fagopyri Esculenti is sweet cherry roots or Radix Et Rhizoma Fagopyri Tatarici；
The preparation method of above-mentioned Semen Fagopyri Esculenti probiotic bacteria solid fermentation functional food, specifically comprises the following steps that
(1), after buckwheat shelling, by Semen Fagopyri Esculenti and water by mass volume ratio it is: 1:0.75-2 prepares solid-state fermentation culture medium,；
(2) solid-state fermentation culture medium being carried out sterilizing, condition is, 121 DEG C, 20min；
(3) actication of culture: mode is with above-mentioned liquid fermentation；
(4) secondary seed solution is accessed in solid-state fermentation culture medium so that Lactobacillus plantarum in every gram of solid medium
Connecing bacterium amount is 1 × 107-5×108CFU/g, inoculates B. animais, Lactobacillus plantarum and animal bifid simultaneously
Bacillus connects bacterium ratio and keeps 1-10:1-10；
(5) mixed culture solid state fermentation 12～48h, 37 DEG C, incubation time 56-92h altogether, pH maintain 7.2～7.4；
After fermentation ends, after being positioned over-80 DEG C of pre-freezes 24 hours, put into vacuum freeze drier 24 hours, system
Obtain Semen Fagopyri Esculenti probiotic bacteria solid fermentation food；
Described Semen Fagopyri Esculenti is sweet cherry roots or Radix Et Rhizoma Fagopyri Tatarici；
Preferably, in solid fermentation, it is 1 × 10 that Lactobacillus plantarum and animal bifidobacteria connect bacterium amount8CFU/g；
Preferably, in solid-state fermentation culture medium, the mass volume ratio of Semen Fagopyri Esculenti and water is 1:1.5；
Preferably, the mixed fungus fermentation time is 30h；
Preferably, described Lactobacillus plantarum is Lactobacillus plantarum (Lactobacillus plantarum) TK9, preservation
Numbered CGMCC No.11891；
Preferably, described animal bifidobacteria is animal bifidobacteria (Bifidobacterium animalis) 937,
Deposit number is CGMCC No.11892.
Beneficial effects of the present invention:
1, Semen Fagopyri Esculenti fermented beverage of the present invention and fermented food, had both remained the nutritive value of Semen Fagopyri Esculenti, again
There is the nutrition health-care functions of probiotics fermention goods.Semen Fagopyri Esculenti is made to obtain unique local flavor by fermentation, and yellow
Letones, total aldehydes matter, reducing sugar, amino-acid nitrogen content etc. are significantly improved, non-oxidizability, ACEI,
DPPIV and alpha-glucosaccharase enzyme inhibition rate isoreactivity are significantly improved so that the reducing blood sugar and blood lipid blood of Semen Fagopyri Esculenti
Effect of pressure is more significantly.This probiotics fermention buckwheat foods enriches the kind of Semen Fagopyri Esculenti product on market.Fermentation
Technique is simple, it is easy to accomplish produce on a large scale.The buckwheat foods of probiotics fermention, compared to Semen Fagopyri Esculenti vinegar, buckwheat
Ale and its nutritional solution, not only low cost technique is simple, it is easy to produce, and target group is bigger.
2, the preparation method of Semen Fagopyri Esculenti fermented beverage of the present invention, according to different age group or different crowd
Human nutrition demand, by Semen Fagopyri Esculenti and different corn collocation fermentation, it is thus achieved that different local flavors and effect.
The Semen Fagopyri Esculenti fermented beverage obtained by this preparation method and food are except containing substantial amounts of nutritive effect material
Outward, in sweet cherry roots fermented product, Lactobacillus plantarum TK9 and the highest viable count of bacillus bifidus 937 can reach respectively
To 5.39 × 109CFU/mL、2.57×109CFU/g (Lactobacillus plantarum TK9) and 1.31 × 109CFU/mL、
1.01×109CFU/g (animal bifidobacteria 937), Lactobacillus plantarum TK9 and bifid in Radix Et Rhizoma Fagopyri Tatarici fermented product
The highest viable count of bacillus 937 can reach 7.25 × 10 respectively9CFU/mL、4.2×109CFU/g (plant breast bar
Bacterium TK9) and 2.12 × 109CFU/mL、1.47×109CFU/g (animal bifidobacteria 937).
Accompanying drawing illustrates:
The impact on probiotic bacteria mixed-strains liquid fermentation sweet cherry roots beverage viable count of Fig. 1: the mixed fungus fermentation incubation time
The impact on probiotic bacteria mixed-strains liquid fermentation Radix Et Rhizoma Fagopyri Tatarici food viable count of Fig. 2: the mixed fungus fermentation incubation time
The impact on probiotic bacteria mixed culture solid state fermentation sweet cherry roots beverage viable count of Fig. 3: the mixed fungus fermentation incubation time
The impact on probiotic bacteria mixed culture solid state fermentation Radix Et Rhizoma Fagopyri Tatarici food viable count of Fig. 4: the mixed fungus fermentation incubation time
Detailed description of the invention:
Below in conjunction with form and specific embodiment, the invention will be further described, but the present invention does not limit
In specific examples below.
Following all actication of culture and liquid fermentation are quiescent culture.
Embodiment 1, probiotic bacteria liquid fermentation researchonthe technology-Semen Fagopyri Esculenti and the determination of corn proportioning
(1) after buckwheat shelling, Semen Fagopyri Esculenti and corn being cleaned, pulverizing crosses 100 mesh sieves；
(2) preparation activation medium: every 100ml water adds 5g Fagopyrum esculentum Moench powder (Fagopyrum esculentum Moench powder in activation medium with
Fagopyrum esculentum Moench powder kind in fermentation medium is consistent)；Activation medium is carried out sterilizing, and condition is, 121 DEG C,
20min；
(3) preparation fermentation medium: add 5g sweet cherry roots powder/Radix Et Rhizoma Fagopyri Tatarici powder, the Semen sojae atricolor of 0-5g in every 100ml water
Powder/oatmeal/wheat flour/Semen Maydis powder/black bean powder, 121 DEG C of sterilizing 20min；
(4) actication of culture, Lactobacillus plantarum and animal bifidobacteria glycerol pipe are inoculated in activation medium by 1% respectively
Activating, pH7.2-7.4, controlling temperature is 37 DEG C；Respectively after activation 20h, by 10% (v/v's)
Connect bacterium amount activation medium of again transferring and carry out re-activation, after reactivation 24h secondary seed solution；
(5) probiotics fermention: be 1 × 10 by connecing bacterium amount by Lactobacillus plantarum7CFU/mL is inoculated in fermentation medium
In, inoculating animal bifidobacteria, Lactobacillus plantarum and animal bifidobacteria simultaneously and connecing bacterium amount volume ratio is 1:1,
Mixed-strains liquid fermentation 24h, temperature is 37 DEG C, and pH maintains 7.2～7.4；Respectively to the probiotic bacteria in fermentation liquid
Viable count detects, result such as table 1-10:
Table 1 sweet cherry roots powder: during Semen sojae atricolor powder=5:0-5, the viable count (CFU/mL) of probiotic bacteria
| Ratio | 5:0 | 5:1 | 5:2 | 5:3 | 5:4 | 5:5 |
| The mono-bacterium of TK9 | 1.05×108 | 5.8×108 | 6.3×108 | 5.4×108 | 5.67×108 | 5.56×108 |
| 937 single bacterium | 4.5×107 | 1.33×108 | 1.8×108 | 1.78×108 | 1.57×108 | 1.64×108 |
| Total viable count | 1.5×108 | 7.13×108 | 8.1×108 | 7.18×108 | 7.24×108 | 7.2×108 |
Table 2 sweet cherry roots powder: during oatmeal=5:0-5, the viable count (CFU/mL) of probiotic bacteria
| Ratio | 5:0 | 5:1 | 5:2 | 5:3 | 5:4 | 5:5 |
| The mono-bacterium of TK9 | 1.05×108 | 1.77×108 | 2.03×108 | 3.0×108 | 2.63×108 | 2.5×108 |
| 937 single bacterium | 4.5×107 | 8.5×107 | 1.4×108 | 1.68×108 | 1.47×108 | 1.46×108 |
| Total viable count | 1.5×108 | 2.62×108 | 3.43×108 | 4.68×108 | 4.1×108 | 3.96×108 |
Table 3 sweet cherry roots powder: during wheat flour=5:0-5, number of live bacteria of probiotics (CFU/mL)
| Ratio | 5:0 | 5:1 | 5:2 | 5:3 | 5:4 | 5:5 |
| The mono-bacterium of TK9 | 1.05×108 | 2.04×108 | 2.93×108 | 2.57×108 | 2.36×108 | 2.51×108 |
| 937 single bacterium | 4.5×107 | 7.5×107 | 1.23×108 | 1.36×108 | 1.46×108 | 1.46×108 |
| Total viable count | 1.5×108 | 2.79×108 | 4.16×108 | 3.93×108 | 3.82×108 | 3.96×108 |
Table 4 sweet cherry roots powder: during Semen Maydis powder=5:0-5, number of live bacteria of probiotics (CFU/mL)
| Ratio | 5:0 | 5:1 | 5:2 | 5:3 | 5:4 | 5:5 |
| The mono-bacterium of TK9 | 1.05×108 | 1.47×108 | 1.84×108 | 2.21×108 | 2.05×108 | 1.92×108 |
| 937 single bacterium | 4.5×107 | 7.45×107 | 9.4×107 | 1.56×108 | 1.57×108 | 1.36×108 |
| Total viable count | 1.5×108 | 2.21×108 | 2.78×108 | 3.77×108 | 3.62×108 | 3.28×108 |
Table 5 sweet cherry roots powder: during black bean powder=5:0-5, number of live bacteria of probiotics (CFU/mL)
Table 6 Radix Et Rhizoma Fagopyri Tatarici powder: Semen sojae atricolor powder joins=5:0-5 time, the viable count (CFU/mL) of probiotic bacteria
| Ratio | 5:0 | 5:1 | 5:2 | 5:3 | 5:4 | 5:5 |
| The mono-bacterium of TK9 | 2.0×108 | 2.75×108 | 4.56×108 | 4.78×108 | 4.34×108 | 3.23×108 |
| 937 single bacterium | 1.5×108 | 2×108 | 2.02×108 | 2.32×108 | 2.06×108 | 2.64×108 |
| Total viable count | 3.5×108 | 4.75×108 | 6.6×108 | 7.1×108 | 6.4×108 | 5.87×108 |
Table 7 Radix Et Rhizoma Fagopyri Tatarici powder: during oatmeal=5:0-5, the viable count (CFU/mL) of probiotic bacteria
| Ratio | 5:0 | 5:1 | 5:2 | 5:3 | 5:4 | 5:5 |
| The mono-bacterium of TK9 | 2.0×108 | 2.64×108 | 3.0×108 | 3.98×108 | 4.23×108 | 4.00×108 |
| 937 single bacterium | 1.5×108 | 2.01×108 | 2.0×108 | 2.02×108 | 2.12×108 | 1.87×108 |
| Total viable count | 3.5×108 | 4.65×108 | 5.0×108 | 6.00×108 | 6.38×108 | 5.87×108 |
Table 8 Radix Et Rhizoma Fagopyri Tatarici powder: during Semen sojae atricolor powder=5:0-5, number of live bacteria of probiotics (CFU/mL)
| Ratio | 5:0 | 5:1 | 5:2 | 5:3 | 5:4 | 5:5 |
| The mono-bacterium of TK9 | 2.0×108 | 2.25×108 | 3.48×108 | 3.78×108 | 3.24×108 | 2.56×108 |
| 937 single bacterium | 1.5×108 | 2.1×108 | 2.02×108 | 2.57×108 | 2.65×108 | 2.87×108 |
| Total viable count | 3.5×108 | 4.35×108 | 5.50×108 | 6.35×108 | 5.89×108 | 5.43×108 |
Table 9 Radix Et Rhizoma Fagopyri Tatarici powder: during Semen Maydis powder=5:0-5, the viable count (CFU/mL) of probiotic bacteria
| Ratio | 5:0 | 5:1 | 5:2 | 5:3 | 5:4 | 5:5 |
| The mono-bacterium of TK9 | 2.0×108 | 2.25×108 | 2.45×108 | 2.45×108 | 2.30×108 | 2.1×108 |
| 937 single bacterium | 1.5×108 | 1.73×108 | 1.76×108 | 2.05×108 | 1.7×108 | 1.76×108 |
| Total viable count | 3.5×108 | 3.98×108 | 4.21×108 | 4.5×108 | 4.0×108 | 3.86×108 |
Table 10 Radix Et Rhizoma Fagopyri Tatarici powder: during black bean powder=5:0-5, the viable count (CFU/mL) of probiotic bacteria
| Ratio | 5:0 | 5:1 | 5:2 | 5:3 | 5:4 | 5:5 |
| The mono-bacterium of TK9 | 2.0×108 | 3.54×108 | 3.98×108 | 4.34×108 | 5.0×108 | 5.3×108 |
| 937 single bacterium | 1.5×108 | 1.58×108 | 2.37×108 | 2.66×108 | 3.00×108 | 3.4×108 |
| Total viable count | 3.5×108 | 5.12×108 | 6.35×108 | 7.0×108 | 8.0×108 | 8.7×108 |
From table 1-10, it is as follows that optimal Fagopyrum esculentum Moench powder and grain dust cultivate formula:
1. sweet cherry roots powder: the ratio of Semen sojae atricolor powder is 5:2, the total viable count recorded is 8.1 × 108CFU/mL, its
Middle Lactobacillus plantarum TK9 viable count is 6.3 × 108CFU/mL, animal bifidobacteria 937 viable count are
1.8×108CFU/mL；
2. sweet cherry roots powder: oatmeal ratio is 5:4, the total viable count recorded is 6.38 × 108CFU/mL, wherein
Lactobacillus plantarum TK9 viable count is 4.23 × 108CFU/mL, animal bifidobacteria 937 viable count are
2.12×108CFU/mL。
3. sweet cherry roots powder: wheat flour ratio is 5:2, the total viable count recorded is 4.16 × 108CFU/mL, wherein
Lactobacillus plantarum TK9 viable count is 2.93 × 108CFU/mL, animal bifidobacteria 937 viable count are 1.23 × 109
CFU/mL。
4. sweet cherry roots powder: Semen Maydis powder ratio is 5:3, the total viable count recorded is 3.77 × 108CFU/mL, wherein
Lactobacillus plantarum TK9 viable count is 2.21 × 108CFU/mL, animal bifidobacteria 937 viable count are
1.56×108CFU/mL。
5. sweet cherry roots powder: black bean powder ratio is 5:5, the total viable count recorded is 7.82 × 108CFU/mL, its
Middle Lactobacillus plantarum TK9 viable count is 5.1 × 108CFU/mL, animal bifidobacteria 937 viable count are
2.62×108CFU/mL。
6. Radix Et Rhizoma Fagopyri Tatarici powder: Semen sojae atricolor powder ratio is 5:3, the total viable count recorded is 7.1 × 108CFU/mL, wherein
Lactobacillus plantarum TK9 viable count is 4.78 × 108CFU/mL, animal bifidobacteria 937 viable count are
2.32×108CFU/mL。
7. Radix Et Rhizoma Fagopyri Tatarici powder: oatmeal ratio is 5:4.The total viable count recorded is 6.38 × 108CFU/mL, wherein
Lactobacillus plantarum TK9 viable count is 4.23 × 108CFU/mL, animal bifidobacteria 937 viable count are
2.12×108CFU/mL。
8. Radix Et Rhizoma Fagopyri Tatarici powder: wheat flour ratio is 5:3, the total viable count recorded is 6.35 × 108CFU/mL, wherein
Lactobacillus plantarum TK9 viable count is 3.78 × 108CFU/mL, animal bifidobacteria 937 viable count are
2.57×108CFU/mL。
9. Radix Et Rhizoma Fagopyri Tatarici powder: Semen Maydis powder ratio is 5:3.The total viable count recorded is 4.5 × 108CFU/mL, wherein
Lactobacillus plantarum TK9 viable count is 2.45 × 108CFU/mL, animal bifidobacteria 937 viable count are
2.05×108CFU/mL。
10. Radix Et Rhizoma Fagopyri Tatarici powder: black bean powder ratio is 5:5, the total viable count recorded is 8.7 × 108CFU/mL, wherein
Lactobacillus plantarum TK9 viable count is 5.3 × 108CFU/mL, animal bifidobacteria 937 viable count are
3.4×108CFU/mL。
Embodiment 2: the determination of probiotic bacteria liquid fermentation researchonthe technology-probiotic bacteria inoculum concentration
Other conditions are with embodiment 1, in sweet cherry roots powder: the ratio of Semen sojae atricolor powder is that 5g:2g configures fermentation medium,
Lactobacillus plantarum is connect bacterium amount 1 × 10 by following6、5×106、1×107、5×107CFU/mL is inoculated in respectively and sends out
In ferment culture medium, inoculate animal bifidobacteria, Lactobacillus plantarum and animal bifidobacteria simultaneously and connect bacterium amount ratio and be
1:1, ferments, and fermented the number of live bacteria of probiotics measuring in fermentation liquid afterwards.
Result is as shown in table 11: optimum inoculation amount Lactobacillus plantarum TK9 and animal bifidobacteria 937 are
5×107CFU/mL.The total viable count recorded is 3.5 × 109CFU/mL, wherein Lactobacillus plantarum TK9 viable bacteria
Number is 2.7 × 109CFU/mL, animal bifidobacteria 937 viable count are 8.01 × 108CFU/mL。
Viable count (CFU/mL) under table 11 different vaccination amount
Other conditions are with embodiment 1, in Radix Et Rhizoma Fagopyri Tatarici powder: Semen sojae atricolor powder ratio is that 5g:3g configures fermentation medium,
Inoculate Lactobacillus plantarum TK9 by different inoculum concentrations simultaneously and animal bifidobacteria 937 ferments, fermentation
Complete the number of live bacteria of probiotics measuring in fermentation liquid afterwards.
Result is as shown in the table: optimum inoculation amount Lactobacillus plantarum TK9 and animal bifidobacteria 937 are
5×107CFU/mL.The total viable count recorded is 3.93 × 109CFU/mL, wherein Lactobacillus plantarum TK9 viable bacteria
Number is 3.04 × 109CFU/mL, animal bifidobacteria 937 viable count are 8.94 × 108CFU/mL。
Viable count (CFU/mL) under table 12 different vaccination amount
Embodiment 3: the determination of probiotic bacteria liquid fermentation researchonthe technology-incubation time
(1) under conditions of inoculum concentration optimizes, liquid fermentation culture medium selects to add in every 100ml water 5g
Sweet cherry roots powder and the Semen sojae atricolor powder of 2g.
Lactobacillus plantarum TK9 and animal bifidobacteria 937 glycerol pipe are inoculated in sweet cherry roots activation medium by 1%
Once activate, all activate 20h, the most all transfer by the inoculum concentration of 10% (v/v) and cultivate into re-activation
In base, re-activation 24h.Inoculate Lactobacillus plantarum TK9 and animal bifid in liquid fermentation culture medium simultaneously
Bacillus 937 seed liquor mixed fungus fermentation.
Make to be linked into Lactobacillus plantarum TK9 and animal bifidobacteria 937 in sweet cherry roots liquid fermentation culture medium,
It is 5 × 107CFU/mL；PH7.2-7.4, cultivation temperature 37 DEG C, mixed fungus fermentation 4,10,16,22,28,
34,40h, detects the number of live bacteria of probiotics in fermentation liquid respectively.
Result: the optimal incubation time of mixed-strains liquid fermentation is 28h, total incubation time is 72h (see accompanying drawing 1).
(2) under conditions of inoculum concentration optimizes, liquid fermentation culture medium selects to add in every 100ml water 5g
Radix Et Rhizoma Fagopyri Tatarici powder and the Semen sojae atricolor powder of 3g.
Lactobacillus plantarum TK9 and animal bifidobacteria 937 glycerol pipe are inoculated in Radix Et Rhizoma Fagopyri Tatarici activation medium by 1%
Once activate, all activate 20h, the most all transfer by the inoculum concentration of 10% (v/v) and cultivate into re-activation
In base, re-activation 24h.Inoculate Lactobacillus plantarum TK9 and animal bifid in liquid fermentation culture medium simultaneously
Bacillus 937 seed liquor mixed fungus fermentation.
Make to be linked into Lactobacillus plantarum TK9 and animal bifidobacteria 937 in Radix Et Rhizoma Fagopyri Tatarici liquid fermentation culture medium,
It is 5 × 107CFU/mL；PH7.2-7.4, cultivation temperature 37 DEG C, mixed fungus fermentation 4,10,16,22,28,
34,40h, detects the number of live bacteria of probiotics in fermentation liquid respectively.
Result: the optimal incubation time of mixed-strains liquid fermentation is 28h, total incubation time is 72h (see accompanying drawing 2).
Embodiment 4: the preparation method of a kind of Semen Fagopyri Esculenti liquid fermentation beverage
(1) after buckwheat shelling, Semen Fagopyri Esculenti and corn being cleaned, pulverizing is crossed 100 mesh sieves and is obtained Fagopyrum esculentum Moench powder and grain dust respectively；
(2) preparation activation medium: add 5g sweet cherry roots powder in every 100ml water；Activation medium is carried out sterilizing,
Condition is, 121 DEG C, 20min；
(3) preparation fermentation medium:: interpolation 5g sweet cherry roots powder in every 100ml water, the Semen sojae atricolor powder of 2g, 121 DEG C
Sterilizing 20min；
(4) actication of culture, Lactobacillus plantarum and animal bifidobacteria glycerol pipe are inoculated in activation medium by 1% respectively
Activating, pH7.2-7.4, controlling temperature is 37 DEG C；Respectively after activation 20h, by 10% (v/v's)
Connect bacterium amount activation medium of again transferring and carry out re-activation, after reactivation 24h secondary seed solution；
(5) probiotics fermention: be 5 × 10 by connecing bacterium amount by Lactobacillus plantarum7CFU/mL is inoculated in fermentation medium
In, inoculating animal bifidobacteria, Lactobacillus plantarum and animal bifidobacteria simultaneously and connecing bacterium amount volume ratio is 1:1,
Mixed-strains liquid fermentation 28h, temperature is 37 DEG C, and pH maintains 7.2～7.4；
(6) fermentation ends after fermentation liquid adds stabilizer after emulsifying, and stabilizer is carried out by with fermentation liquid mass volume ratio
Adding, addition is sodium carboxymethyl cellulose 0.9%, xanthan gum 0.4%, emulsifying agent 0.9%；Add and fermentation
Liquid mass volume ratio be 8% sucrose carry out acid adjustment perfumery；At 65 DEG C, under conditions of 30MPa, homogenizing is once,
Through 90 DEG C of 15min that sterilize, obtain Semen Fagopyri Esculenti fermented beverage.
Embodiment 5: culture medium active component change (flavone before and after probiotic bacteria liquid fermentation researchonthe technology-fermentation
Class material, total phenols, ACEI, GABA, polyphenoils content, reducing sugar, amino nitrogen, DPPIV
Inhibitory activity (DIR), alpha-Glucosidase inhibitory activity (α-GIR))
Following test with sweet cherry roots and Radix Et Rhizoma Fagopyri Tatarici for fermentation substrate respectively::
(1) after buckwheat shelling, being cleaned by Semen Fagopyri Esculenti, pulverizing crosses 100 mesh sieves；
(2) preparation activation medium: add 5g sweet cherry roots powder/Radix Et Rhizoma Fagopyri Tatarici powder in every 100ml water (in activation medium
Fagopyrum esculentum Moench powder consistent with the Fagopyrum esculentum Moench powder kind in fermentation medium)；Activation medium is carried out sterilizing, and condition is,
121 DEG C, 20min；
(3) preparation fermentation medium: add 5g sweet cherry roots powder/Radix Et Rhizoma Fagopyri Tatarici powder, 121 DEG C of sterilizings in every 100ml water
20min；
(4) actication of culture, Lactobacillus plantarum and animal bifidobacteria glycerol pipe are inoculated in activation medium by 1% respectively
Activating, pH7.2-7.4, controlling temperature is 37 DEG C；Respectively after activation 20h, by 10% (v/v's)
Connect bacterium amount activation medium of again transferring and carry out re-activation, after reactivation 24h secondary seed solution；
(5) probiotics fermention: be 1 × 10 by connecing bacterium amount by Lactobacillus plantarum7CFU/mL is inoculated in fermentation medium
In, inoculating animal bifidobacteria, Lactobacillus plantarum and animal bifidobacteria simultaneously and connecing bacterium amount volume ratio is 1:1,
Mixed-strains liquid fermentation 24h, temperature is 37 DEG C, and pH maintains 7.2～7.4, and during mixed fungus fermentation, strain is respectively as follows:
TK9,937 mixed fungus fermentations and Lactobacillus plantarum CGMCCNo.1.6971, animal bifidobacteria CGMCC
No.1.3003 mixed fungus fermentation, compares with single bacterium fermentation, and during single bacterium fermentation, inoculum concentration is 2 × 107CFU/mL。
Utilize Syrups by HPLC GABA and Flavonoid substances content；Utilize forint phenol reaction assay total phenols
Class content；Oxygen index method (ORAC) of living is utilized to measure polyphenoils content；With reference to Hyun and Shin[2]Deng
Assay method in people's article measures ACEI content (Utilization of bovine blood plasma proteins
for the production of angotensin I converting enzyme inhibitory peptides.)；With reference to Li Meng
Cover and Wu Wei et al. article " different yeast solid fermentation are on Semen Fagopyri Esculenti antioxidant content and the impact of antioxidation "
In assay method measure antioxidant activity；DNS method is utilized to measure content of reducing sugar；Utilize triketohydrindene hydrate
Method measures amino-acid nitrogen content；With reference to Zhu zeng and Junyi et al. article " Screening for potential
novel probiotic Lactobacillus strains based on high dipeptidyl peptidase IV and
α-glucosidase inhibitory activity " in assay method measure DPPIV and alpha-Glucosidase suppression live
Property.
Table 13: active component change before and after sweet cherry roots liquid fermentation
Table 14: active component change before and after Radix Et Rhizoma Fagopyri Tatarici liquid fermentation
From two above data, Semen Fagopyri Esculenti is all improved at active component after liquid fermentation, wherein Radix Et Rhizoma Fagopyri Tatarici
Become apparent from.GABA content increased after fermented, and after sweet cherry roots mixed fungus fermentation, GABA content reaches
To for 1.69mg/ml, after Radix Et Rhizoma Fagopyri Tatarici mixed fungus fermentation, GABA content is reached for 2.2mg/ml, is above single bacterium
Fermentation, hypertension is had by this composition to be alleviated and effect for the treatment of；After mixed fungus fermentation, during sweet cherry roots beverage converges
Total phenols content reaches 1.85mg GAE/ml, and the total phenols content during Radix Et Rhizoma Fagopyri Tatarici beverage converges reaches 9.78mg
GAE/ml, is above single bacterium fermentation, and total aldehydes matter all has anti-oxidation efficacy；Antioxygen after mixed bacterium after fermentation
Change activity to significantly improve, the twice before being wherein its fermentation with sweet cherry roots after probiotics fermention for fermentation substrate,
Before reaching its fermentation with Radix Et Rhizoma Fagopyri Tatarici after probiotics fermention for fermentation substrate three times, send out apparently higher than single bacterium simultaneously
Ferment；In Radix Et Rhizoma Fagopyri Tatarici, Flavone content is apparently higher than in sweet cherry roots, about 5 times, after mixed fungus fermentation, and Radix Et Rhizoma Fagopyri Tatarici
Middle Flavonoid substances content substantially increases, and content reaches 0.85mg/ml, and in sweet cherry roots, content also increased,
Reaching 0.34mg/ml, Flavonoid substances has reducing blood sugar and blood lipid and anti-oxidation efficacy, the flavonoid thing in Radix Et Rhizoma Fagopyri Tatarici
Matter be mainly composed of rutin (70%-90%), its in other corn almost without, be mainly used in clinically
Diabetes, the auxiliary treatment of hyperglycemia；In Semen Fagopyri Esculenti mixed fungus fermentation beverage, ACEI content increases relative to single bacterium fermentation
Add notable, wherein after probiotics fermention, reach 12.7% with sweet cherry roots for fermentation substrate, with Radix Et Rhizoma Fagopyri Tatarici for fermentation base
Matter reaches 22% after probiotics fermention, has effect of good blood pressure lowering.In Semen Fagopyri Esculenti mixed fungus fermentation beverage also
Raw sugar and amino-acid nitrogen content are relative to not fermenting Semen Fagopyri Esculenti and single bacterium fermentation increases notable, wherein content of reducing sugar
Relatively all increasing by more than six times before fermentation, amino-acid nitrogen content all increases more than octuple before relatively fermenting.Semen Fagopyri Esculenti mixes bacterium and sends out
Ferment beverage is relative to Semen Fagopyri Esculenti and use single bacterium fermentation, its DPPIV and the alpha-Glucosidase inhibitory activity of not fermenting
High.DPPIV has hydrolysis to gut incretin hormones, it is suppressed that the effect that these hormones are brought into normal play,
DPPIV inhibitor can suppress DPPIV activity, reduces it and gut incretin hormones is had hydrolysis, right
Blood sugar lowering plays positive role.Alpha-glucosidase can be from non-reducing end hydrolysis oligosaccharide and polysaccharide
α-Isosorbide-5-Nitrae-glucoside bond, is possible to prevent blood glucose to raise its suppression.Result shows to utilize mixed fungus fermentation to open
Send the Semen Fagopyri Esculenti functional drinks that nutrition is more rich.
Embodiment 6: the determination of solid-state fermentation process-strain inoculum concentration
(1) viable count under sweet cherry roots solid medium different vaccination amount
Lactobacillus plantarum TK9 and animal bifidobacteria 937 glycerol pipe are inoculated in sweet cherry roots activation training by 1% respectively
(adding 5g sweet cherry roots powder in every 100ml water, sterilising conditions is, 121 DEG C, 20min to support base；) carry out once
Activation；All activate 20h, the most all transfer by the bacterium amount that connects of 10% (v/w) (same into re-activation culture medium
Activation medium) in, re-activation 24h, sweet cherry roots solid-state fermentation culture medium (after buckwheat shelling,
Being that 1:1 (mass volume ratio) prepares solid-state fermentation culture medium by Semen Fagopyri Esculenti and water material-water ratio, sterilising conditions is,
121 DEG C, 20min) in；Inoculation Lactobacillus plantarum TK9 and animal bifidobacteria 937 seed liquor are carried out simultaneously
Mixed fungus fermentation, mixed culture solid state fermentation 24h, 37 DEG C, incubation time 68h altogether, pH maintain 7.2～7.4.
Number of live bacteria of probiotics in fermentation wild Oryza species is detected.
Inoculum concentration: Lactobacillus plantarum (L.plantarum TK9) and animal bifidobacteria 937 (B.animalis
937) inoculum concentration of two strain bacterium is respectively: 1 × 107CFU/mL；5×107CFU/mL；1×108CFU/mL；
5×108CFU/mL。
Result is as shown in the table: Lactobacillus plantarum (L.plantarum TK9) and animal bifidobacteria 937 (B.
Animalis 937) optimum inoculation amount of two strain bacterium is 1 × 108CFU/g, i.e. records gained sweet cherry roots probiotic bacteria and sends out
Total viable count in ferment functional food is 1.82 × 109CFU/g.Wherein, Lactobacillus plantarum viable count is
1.28×109CFU/g, the viable count of animal bifidobacteria reaches 5.37 × 108CFU/g。
Viable count (CFU/g) under table 15 sweet cherry roots solid fermentation different vaccination amount
(2) viable count under Radix Et Rhizoma Fagopyri Tatarici solid medium different vaccination amount
Lactobacillus plantarum TK9 and animal bifidobacteria 937 glycerol pipe are inoculated in Radix Et Rhizoma Fagopyri Tatarici activation training by 1% respectively
Support base (adding 5g Radix Et Rhizoma Fagopyri Tatarici powder in every 100ml water, sterilising conditions is, 121 DEG C, 20min), carry out once
Activation, all activates 20h, the most all transfers by the bacterium amount that connects of 10% (v/w) (same into re-activation culture medium
Activation medium) in, re-activation 24h, in Radix Et Rhizoma Fagopyri Tatarici solid-state fermentation culture medium, (material-water ratio is: 1:1)
Middle inoculation Lactobacillus plantarum TK9 and animal bifidobacteria 937 seed liquor simultaneously carry out mixed fungus fermentation.
Inoculum concentration and condition of culture: Lactobacillus plantarum (L.plantarum TK9) and animal bifidobacteria 937
The inoculum concentration of (B.animalis 937) two strain bacterium is respectively: 1 × 107CFU/g；5×107CFU/g；
1×108CFU/g；5×108CFU/g.Mixed culture solid state fermentation 24h, 37 DEG C, incubation time 68h altogether, pH
Maintain 7.2～7.4.Number of live bacteria of probiotics in fermentation wild Oryza species is detected.
Result is as shown in the table: optimum inoculation amount is 1 × 108CFU/g, i.e. records gained Radix Et Rhizoma Fagopyri Tatarici probiotic bacteria and sends out
Total viable count in ferment functional food is 2.26 × 109CFU/g.Wherein, Lactobacillus plantarum viable count is
1.56×109CFU/g, the viable count of animal bifidobacteria reaches 6.95 × 108CFU/g。
Table 16: the viable count (CFU/g) under sweet cherry roots solid fermentation different vaccination amount
Embodiment 7: the determination of solid-state fermentation process-amount of water
(1) sweet cherry roots solid-state fermentation culture medium: the ratio (mass volume ratio) of sweet cherry roots and water is respectively
1:0.75,1:1,1:1.25,1:1.5,1:1.75,1:2.I.e. sweet cherry roots 40g correspondence 30,40,50,60,
70,80ml water.
Lactobacillus plantarum TK9 and animal bifidobacteria 937 glycerol pipe are inoculated in sweet cherry roots activation training by 1% respectively
Support base (adding 5g sweet cherry roots powder in every 100ml water, sterilising conditions is, 121 DEG C, 20min), carry out once
Activation, all activates 20h, the most all transfers by the bacterium amount that connects of 10% (v/w) (same into re-activation culture medium
Activation medium) in, re-activation 24h, inoculates plant in sweet cherry roots solid-state fermentation culture medium simultaneously
Lactobacillus TK9 and animal bifidobacteria 937 seed liquor, inoculum concentration is 1 × 10 respectively8CFU/mL, is carried out
Mixed fungus fermentation.Condition of culture: 24h, 37 DEG C, incubation time 68h altogether, pH maintain 7.2～7.4.Right
Number of live bacteria of probiotics in fermentation wild Oryza species detects.
Result is as shown in the table: optimal material-water ratio is 1:1.5, i.e. records gained sweet cherry roots probiotics fermention function
Total viable count in property food is 3.49 × 109CFU/g.Wherein, Lactobacillus plantarum viable count is
2.34×109CFU/g, the viable count of animal bifidobacteria reaches 1.15 × 109CFU/g。
When the proportioning of table 17 sweet cherry roots and water is 1:0.75-2, number of live bacteria of probiotics (CFU/g)
| Ratio | 1:0.75 | 1:1 | 1:1.25 | 1:1.5 | 1:1.75 | 1:2 |
| The mono-bacterium of TK9 | 5.83×108 | 1.28×109 | 1.87×109 | 2.34×109 | 2.04×109 | 1.79×109 |
| 937 single bacterium | 1.58×108 | 5.37×108 | 1.04×109 | 1.25×109 | 1.15×109 | 9.3×108 |
| Total viable count | 7.41×108 | 1.82×109 | 2.91×109 | 3.59×109 | 3.19×109 | 2.72×109 |
(2) Radix Et Rhizoma Fagopyri Tatarici solid-state fermentation culture medium: the ratio of Radix Et Rhizoma Fagopyri Tatarici and water is respectively 1:0.75,1:1,1:1.25,
1:1.5,1:1.75,1:2.I.e. Radix Et Rhizoma Fagopyri Tatarici 40g correspondence 30,40,50,60,70,80ml water.
Lactobacillus plantarum TK9 and animal bifidobacteria 937 glycerol pipe are inoculated in Radix Et Rhizoma Fagopyri Tatarici activation training by 1% respectively
Support base (adding 5g Radix Et Rhizoma Fagopyri Tatarici powder in every 100ml water, sterilising conditions is, 121 DEG C, 20min) to carry out once
Activation, all activates 20h, the most all transfers by the bacterium amount that connects of 10% (v/w) (same into re-activation culture medium
Activation medium) in, re-activation 24h, inoculates plant in Radix Et Rhizoma Fagopyri Tatarici solid-state fermentation culture medium simultaneously
Lactobacillus TK9 and animal bifidobacteria 937 seed liquor, inoculum concentration is 1 × 10 respectively8CFU/mL, is carried out
Mixed fungus fermentation.Condition of culture: 24h, 37 DEG C, incubation time 68h altogether, pH maintain 7.2～7.4.Right
Number of live bacteria of probiotics in fermentation wild Oryza species detects.
Result is as shown in the table: optimal material-water ratio is 1:1.5, i.e. records gained Radix Et Rhizoma Fagopyri Tatarici probiotics fermention function
Total viable count in property food is 3.49 × 109CFU/g.Wherein, Lactobacillus plantarum viable count is
2.34×109CFU/mL, the viable count of animal bifidobacteria reaches 1.15 × 109CFU/mL。
When the proportioning of table 18 Radix Et Rhizoma Fagopyri Tatarici and water is 1:0.75-2, number of live bacteria of probiotics (CFU/g)
| Ratio | 1:0.75 | 1:1 | 1:1.25 | 1:1.5 | 1:1.75 | 1:2 |
| The mono-bacterium of TK9 | 7.41×108 | 1.56×109 | 2.01×109 | 2.34×109 | 1.89×109 | 1.88×109 |
| 937 single bacterium | 2.48×108 | 6.95×108 | 1.04×109 | 1.15×109 | 1.06×109 | 9.8×108 |
| Total viable count | 9.89×108 | 2.26×109 | 3.05×109 | 3.49×109 | 2.95×109 | 2.86×109 |
Embodiment 8: the determination of solid-state fermentation process-incubation time
(1) Lactobacillus plantarum TK9 and animal bifidobacteria 937 glycerol pipe are inoculated in sweet cherry roots activation by 1% respectively
Culture medium (adding 5g sweet cherry roots powder in every 100ml water, sterilising conditions is, 121 DEG C, 20min) carries out one
Secondary activation, all activates 20h, the most all transfers by the bacterium amount that connects of 10% (v/w) (same into re-activation culture medium
Activation medium) in, re-activation 24h, at the sweet cherry roots solid state fermentation culture that material-water ratio is the most optimized
Inoculating Lactobacillus plantarum TK9 and animal bifidobacteria 937 seed liquor in base, inoculum concentration is respectively simultaneously
1×108CFU/g, carries out mixed fungus fermentation.Respectively mixed fungus fermentation 12,18,24,30,36,42,48h,
37 DEG C, incubation time 56-92h altogether, pH maintain 7.2～7.4.Probiotic bacteria in fermentation wild Oryza species is lived
Bacterium number detects.
Shown in result as accompanying drawing 3: optimal incubation time is mixed fungus fermentation 30h, i.e. optimal total fermentation again after inoculation
Time is 74h.
(2) Lactobacillus plantarum TK9 and animal bifidobacteria 937 glycerol pipe are inoculated in Radix Et Rhizoma Fagopyri Tatarici by 1% respectively
Activation medium (adding 5g Radix Et Rhizoma Fagopyri Tatarici powder in every 100ml water, sterilising conditions is, 121 DEG C, 20min) enters
Row once activates, and all activates 20h, the most all transfers by the bacterium amount that connects of 10% (v/w) and cultivate into re-activation
In base (with an activation medium), re-activation 24h, at the Radix Et Rhizoma Fagopyri Tatarici solid fermentation of material-water ratio 1:1.5
Inoculating Lactobacillus plantarum TK9 and animal bifidobacteria 937 seed liquor in culture medium, inoculum concentration is the most simultaneously
It is 1 × 108CFU/mL, carries out mixed fungus fermentation.Respectively mixed fungus fermentation 12,18,24,30,36,42,
48h, 37 DEG C, incubation time 56-92h altogether, pH maintain 7.2～7.4.To the benefit in fermentation wild Oryza species
Raw bacterium viable count detects.
Shown in result as accompanying drawing 4: optimal incubation time is mixed fungus fermentation 30h, i.e. optimal total fermentation again after inoculation
Time is 74h.
Embodiment 9: culture medium active component change before and after solid-state fermentation process-fermentation is (Flavonoid substances, total
Phenols, ACEI, GABA, polyphenoils content, reducing sugar, amino nitrogen)
Following test with sweet cherry roots and Radix Et Rhizoma Fagopyri Tatarici for fermentation substrate respectively:
Lactobacillus plantarum TK9 and animal bifidobacteria 937 glycerol pipe are inoculated in Semen Fagopyri Esculenti activation culture by 1% respectively
Base once activates, and all activates 20h, the most all transfers into re-activation by the bacterium amount that connects of 10% (v/w)
In culture medium (with an activation medium), re-activation 24h, in the Semen Fagopyri Esculenti solid-state that material-water ratio is 1:1.5
Inoculating Lactobacillus plantarum TK9 and animal bifidobacteria 937 seed liquor in fermentation medium, inoculum concentration is divided simultaneously
It is not 1 × 108CFU/g, carries out mixed fungus fermentation, 30h, and 37 DEG C, incubation time 74h altogether, pH maintain
7.2～7.4, during single bacterium fermentation, inoculum concentration is 2 × 108CFU/g, other conditions are constant.
Sweet cherry roots activation medium: sweet cherry roots shelling, cleaning, pulverizing obtain sweet cherry roots powder after crossing 100 mesh sieves, often
Adding 5g sweet cherry roots powder in 100ml water, sterilising conditions is, 121 DEG C, 20min
Sweet cherry roots solid-state fermentation culture medium: after sweet cherry roots shelling by sweet cherry roots and water by mass volume ratio be: 1:1.5
Prepare solid-state fermentation culture medium；Sterilising conditions is, 121 DEG C, 20min；
Radix Et Rhizoma Fagopyri Tatarici activation medium: Fagopyrum tataricum hulling, cleaning, pulverizing obtain Radix Et Rhizoma Fagopyri Tatarici powder after crossing 100 mesh sieves, often
Adding 5g Radix Et Rhizoma Fagopyri Tatarici powder in 100ml water, sterilising conditions is, 121 DEG C, 20min
Radix Et Rhizoma Fagopyri Tatarici solid-state fermentation culture medium: after Fagopyrum tataricum hulling, by Radix Et Rhizoma Fagopyri Tatarici and water by mass volume ratio be: 1:1.5
Prepare solid-state fermentation culture medium；Sterilising conditions is, 121 DEG C, 20min；
Lactobacillus plantarum TK9 and animal bifidobacteria 937 are replaced with CGMCCNo.1.6971 and
CGMCC No.1.3003 repeats the experiment of above-mentioned mixed fungus fermentation.
Utilize Syrups by HPLC GABA and Flavonoid substances content；Utilize forint phenol reaction assay total phenols
Class content；Oxygen index method (ORAC) of living is utilized to measure polyphenoils content；With reference to Hyun and Shin[2]Deng
Assay method in people's article measures ACEI content；With reference to the mensuration side in Li Mengmeng and Wu Wei et al. article
Method measures antioxidant activity；DNS method is utilized to measure content of reducing sugar；Triketohydrindene hydrate method is utilized to measure amino
State nitrogen content；With reference to Zhu zeng and Junyi[3]Et al. assay method in article measure DPPIV and α-glucose
Glycosides enzyme inhibition activity.
Active component change before and after table 19 sweet cherry roots solid fermentation
As shown in Table 19, sweet cherry roots is after solid fermentation, and its GABA content increases, after mixed fungus fermentation
Reach 4.213mg/g, individually ferment higher than two kinds of bacterial strains；Total phenols and polyphenoils after mixed fungus fermentation contain
Amount respectively reaches 11.47mg GAE/g and 0.177mmol TE/g, is above single bacterium fermentation；Mixed bacterium after fermentation
Rear antioxidant activity significantly improves, and reaches four times before fermentation, simultaneously apparently higher than single bacterium fermentation；After fermentation,
Flavonoid substances content also dramatically increases, and wherein mixed fungus fermentation effect is higher than single bacterium fermentation, and content reaches 3.94
mg/g；After mixed fungus fermentation, ACEI content is 45.6%, the result individually fermented higher than two kinds of probiotic bacterias.Semen Fagopyri Esculenti
In mixed fungus fermentation food, reducing sugar and amino-acid nitrogen content are relative to not fermenting Semen Fagopyri Esculenti and single bacterium fermentation increases aobvious
Write, be more beneficial for absorbing of nutrient substance.Sweet cherry roots after solid fermentation, DPPIV and α-glucoside
Enzyme inhibition activity improves, and wherein mixed fungus fermentation effect is better than single bacterium.
Active component change before and after table 20 Radix Et Rhizoma Fagopyri Tatarici solid fermentation
As shown in Table 20, in Radix Et Rhizoma Fagopyri Tatarici solid fermentation food, GABA content increases notable, up to 8.130mg/g,
Higher than single bacterium fermentation；Total phenols and polyphenoils content after mixed fungus fermentation respectively reach 59.7mg GAE/g
With 0.683mmol TE/g, it is above single bacterium fermentation；After mixed bacterium after fermentation, antioxidant activity significantly improves, and reaches
Before fermentation three times, simultaneously apparently higher than single bacterium fermentation；After mixed fungus fermentation, Flavonoid substances is relative to list
Bacterium fermentation increases notable, and content reaches 14.1mg/g；In Semen Fagopyri Esculenti fermented food, ACEI content is relative to single bacterium
Fermentation increases notable, reaches 97.9%.In Radix Et Rhizoma Fagopyri Tatarici mixed fungus fermentation beverage reducing sugar and amino-acid nitrogen content relative to
Do not ferment Semen Fagopyri Esculenti and the fermentation of single bacterium increases notable.Sweet cherry roots after solid fermentation, DPPIV and α-glucoside
Enzyme inhibition activity improves, and wherein mixed fungus fermentation effect is better than single bacterium.Thus, mixed fungus fermentation compares single bacterium fermentation
Can preferably develop the health care of buckwheat foods.
Claims (10)
1. a Semen Fagopyri Esculenti fermented food, it is characterised in that described Semen Fagopyri Esculenti fermented food be with Semen Fagopyri Esculenti or Semen Fagopyri Esculenti with corn as fermentation substrate,
Lactobacillus plantarum and animal bifidobacteria is used to be prepared after liquid or solid-state mixed fungus fermentation；Described Semen Fagopyri Esculenti is sweet cherry roots or hardship
Semen Fagopyri Esculenti.
2. a kind of Semen Fagopyri Esculenti fermented food as claimed in claim 1, it is characterised in that described Lactobacillus plantarum is Lactobacillus plantarum
(Lactobacillus plantarum) TK9, deposit number is CGMCC No.11891；Described animal bifidobacteria is animal
Bacillus bifidus (Bifidobacterium animalis) 937, deposit number is CGMCC No.11892.
3. a kind of Semen Fagopyri Esculenti fermented food as claimed in claim 1 or 2, it is characterised in that described Semen Fagopyri Esculenti fermented food is with liquid fermentation
Prepared by method, specific as follows:
(1) after buckwheat shelling, Semen Fagopyri Esculenti and corn being cleaned, pulverizing is crossed 100 mesh sieves and is obtained Fagopyrum esculentum Moench powder and grain dust respectively；
(2) preparation activation medium: add 5g Fagopyrum esculentum Moench powder in every 100ml water；Activation medium is carried out sterilizing, and condition is, 121 DEG C,
20min；
(3) preparation fermentation medium: add 5-10g Fagopyrum esculentum Moench powder, the grain dust of 0-5g in every 100ml water；Described corn be Herba bromi japonici,
At least one in Semen Tritici aestivi, corn and soybean, Semen sojae atricolor, 121 DEG C of sterilizing 20min；
(4) actication of culture, Lactobacillus plantarum and animal bifidobacteria glycerol pipe are inoculated in activation medium by 1% respectively and activate,
PH7.2-7.4, controlling temperature is 37 DEG C；Respectively after activation 20h, again transfer activation culture by the bacterium amount that connects of 10% (v/v)
Base carries out re-activation, obtains secondary seed solution after reactivation 24h；
(5) probiotics fermention: be 1 × 10 by connecing bacterium amount by Lactobacillus plantarum6-5×107CFU/mL is inoculated in fermentation medium, with
Time inoculation animal bifidobacteria, it is 1-10:1-10 that Lactobacillus plantarum and animal bifidobacteria connect bacterium amount volume ratio, and mixed bacterium solution state stands
Fermentation 4～40h, temperature is 37 DEG C, and pH maintains 7.2～7.4；
(6) fermentation ends after fermentation liquid through emulsifying, add stabilizer, acid adjustment, perfumery, homogenizing, sterilization after i.e. obtain Semen Fagopyri Esculenti probiotic bacteria liquid
Fermented beverage.
4. a kind of Semen Fagopyri Esculenti fermented food as claimed in claim 3, it is characterised in that consisting of of described fermentation medium: every 100ml
Water adds 5g sweet cherry roots powder, the Semen sojae atricolor powder of 2g.
5. a kind of Semen Fagopyri Esculenti fermented food as claimed in claim 3, it is characterised in that described Lactobacillus plantarum and animal bifidobacteria connect
The amount of kind is 5 × 107CFU/mL。
6. a kind of Semen Fagopyri Esculenti fermented food as claimed in claim 3, it is characterised in that the described mixed-strains liquid fermentation time is 28h.
7. a kind of Semen Fagopyri Esculenti fermented food as claimed in claim 1 or 2, it is characterised in that described Semen Fagopyri Esculenti fermented food is with solid fermentation
Prepared by method, specific as follows:
(1), after buckwheat shelling, by Semen Fagopyri Esculenti and water by mass volume ratio it is: 1:0.75-2 prepares solid-state fermentation culture medium；
(2) solid-state fermentation culture medium being carried out sterilizing, condition is, 121 DEG C, 20min；
(3) actication of culture: Lactobacillus plantarum and animal bifidobacteria glycerol pipe are inoculated in activation medium by 1% respectively and activate,
PH7.2-7.4, controlling temperature is 37 DEG C；Respectively after activation 20h, again transfer activation culture by the bacterium amount that connects of 10% (v/v)
Base carries out re-activation, obtains secondary seed solution after reactivation 24h；Activation medium: add 5g Fagopyrum esculentum Moench powder in every 100ml water；
Activation medium is carried out sterilizing, and condition is, 121 DEG C, 20min；Semen Fagopyri Esculenti pulverizing is crossed 100 mesh sieves and obtains Fagopyrum esculentum Moench powder；
(4) secondary seed solution is accessed in solid-state fermentation culture medium so that in every gram of solid medium, Lactobacillus plantarum connects bacterium amount and is
1×107-5×108CFU/g, inoculates B. animais, Lactobacillus plantarum and animal bifidobacteria simultaneously and connects the holding of bacterium ratio
1-10:1-10；
(5) mixed culture solid state fermentation 12～48h, 37 DEG C, pH maintains 7.2～7.4；After fermentation ends, it is positioned over-80 DEG C of pre-freezes 24
After hour, put into vacuum freeze drier 24 hours, prepare Semen Fagopyri Esculenti probiotic bacteria solid fermentation food.
8. a kind of Semen Fagopyri Esculenti fermented food as claimed in claim 7, it is characterised in that described Lactobacillus plantarum and animal bifidobacteria connect
Bacterium amount is 1 × 108CFU/g。
9. a kind of Semen Fagopyri Esculenti fermented food as claimed in claim 7, it is characterised in that Semen Fagopyri Esculenti and water in described solid-state fermentation culture medium
Mass volume ratio is 1:1.5.
10. a kind of Semen Fagopyri Esculenti fermented food as claimed in claim 7, it is characterised in that the described mixed fungus fermentation time is 30h.
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