CN104814502A - Probiotics cereal beverage and powder as well as preparation method thereof - Google Patents
Probiotics cereal beverage and powder as well as preparation method thereof Download PDFInfo
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- CN104814502A CN104814502A CN201510284945.5A CN201510284945A CN104814502A CN 104814502 A CN104814502 A CN 104814502A CN 201510284945 A CN201510284945 A CN 201510284945A CN 104814502 A CN104814502 A CN 104814502A
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- probio
- cfu
- cereal
- cereal beverage
- lactobacillus plantarum
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- 235000013339 cereals Nutrition 0.000 title claims abstract description 120
- 235000013361 beverage Nutrition 0.000 title claims abstract description 80
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 239000006041 probiotic Substances 0.000 title abstract description 14
- 235000018291 probiotics Nutrition 0.000 title abstract description 14
- 239000000843 powder Substances 0.000 title abstract description 5
- 241000894006 Bacteria Species 0.000 claims abstract description 53
- 239000007788 liquid Substances 0.000 claims abstract description 24
- 239000004382 Amylase Substances 0.000 claims abstract description 20
- 102000013142 Amylases Human genes 0.000 claims abstract description 20
- 108010065511 Amylases Proteins 0.000 claims abstract description 20
- 108091005804 Peptidases Proteins 0.000 claims abstract description 20
- 239000004365 Protease Substances 0.000 claims abstract description 20
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 20
- 235000019418 amylase Nutrition 0.000 claims abstract description 20
- 239000002994 raw material Substances 0.000 claims abstract description 15
- 239000006228 supernatant Substances 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 241001465754 Metazoa Species 0.000 claims description 42
- 241000186000 Bifidobacterium Species 0.000 claims description 40
- 244000068988 Glycine max Species 0.000 claims description 40
- 235000010469 Glycine max Nutrition 0.000 claims description 40
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 39
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 39
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 39
- 230000001954 sterilising effect Effects 0.000 claims description 27
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 24
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 24
- 235000007319 Avena orientalis Nutrition 0.000 claims description 20
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 16
- 102000004190 Enzymes Human genes 0.000 claims description 16
- 108090000790 Enzymes Proteins 0.000 claims description 16
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 14
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 10
- 238000011534 incubation Methods 0.000 claims description 10
- 239000002244 precipitate Substances 0.000 claims description 9
- 239000003223 protective agent Substances 0.000 claims description 9
- 239000011159 matrix material Substances 0.000 claims description 8
- 229910052757 nitrogen Inorganic materials 0.000 claims description 8
- 238000011081 inoculation Methods 0.000 claims description 7
- 240000007594 Oryza sativa Species 0.000 claims description 6
- 235000007164 Oryza sativa Nutrition 0.000 claims description 6
- 241000209140 Triticum Species 0.000 claims description 6
- 235000021307 Triticum Nutrition 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 6
- 235000009566 rice Nutrition 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 244000056139 Brassica cretica Species 0.000 claims description 3
- 235000003351 Brassica cretica Nutrition 0.000 claims description 3
- 235000003343 Brassica rupestris Nutrition 0.000 claims description 3
- 241000371652 Curvularia clavata Species 0.000 claims description 3
- 244000062793 Sorghum vulgare Species 0.000 claims description 3
- 244000046109 Sorghum vulgare var. nervosum Species 0.000 claims description 3
- 240000004922 Vigna radiata Species 0.000 claims description 3
- 235000010721 Vigna radiata var radiata Nutrition 0.000 claims description 3
- 235000011469 Vigna radiata var sublobata Nutrition 0.000 claims description 3
- 240000001417 Vigna umbellata Species 0.000 claims description 3
- 235000011453 Vigna umbellata Nutrition 0.000 claims description 3
- 240000008042 Zea mays Species 0.000 claims description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 3
- QKSKPIVNLNLAAV-UHFFFAOYSA-N bis(2-chloroethyl) sulfide Chemical compound ClCCSCCCl QKSKPIVNLNLAAV-UHFFFAOYSA-N 0.000 claims description 3
- 235000021329 brown rice Nutrition 0.000 claims description 3
- 235000005822 corn Nutrition 0.000 claims description 3
- 235000019713 millet Nutrition 0.000 claims description 3
- 235000010460 mustard Nutrition 0.000 claims description 3
- 241000209763 Avena sativa Species 0.000 claims description 2
- 235000007558 Avena sp Nutrition 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 17
- 230000008569 process Effects 0.000 abstract description 11
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- 230000000529 probiotic effect Effects 0.000 abstract description 3
- 239000004480 active ingredient Substances 0.000 abstract 1
- 238000000855 fermentation Methods 0.000 description 41
- 230000004151 fermentation Effects 0.000 description 41
- 235000013325 dietary fiber Nutrition 0.000 description 15
- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 description 9
- 229920002498 Beta-glucan Polymers 0.000 description 9
- OAKJQQAXSVQMHS-UHFFFAOYSA-N Hydrazine Chemical compound NN OAKJQQAXSVQMHS-UHFFFAOYSA-N 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 7
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 6
- 238000010521 absorption reaction Methods 0.000 description 5
- CJWQYWQDLBZGPD-UHFFFAOYSA-N isoflavone Natural products C1=C(OC)C(OC)=CC(OC)=C1C1=COC2=C(C=CC(C)(C)O3)C3=C(OC)C=C2C1=O CJWQYWQDLBZGPD-UHFFFAOYSA-N 0.000 description 5
- 235000008696 isoflavones Nutrition 0.000 description 5
- 235000016709 nutrition Nutrition 0.000 description 5
- 241000233866 Fungi Species 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000004108 freeze drying Methods 0.000 description 4
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- 235000010987 pectin Nutrition 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 239000003381 stabilizer Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
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- 229930182478 glucoside Natural products 0.000 description 3
- 150000008131 glucosides Chemical class 0.000 description 3
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- 239000002054 inoculum Substances 0.000 description 3
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- 235000014655 lactic acid Nutrition 0.000 description 3
- 239000004310 lactic acid Substances 0.000 description 3
- 230000035764 nutrition Effects 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 238000010563 solid-state fermentation Methods 0.000 description 3
- DKVBOUDTNWVDEP-NJCHZNEYSA-N teicoplanin aglycone Chemical compound N([C@H](C(N[C@@H](C1=CC(O)=CC(O)=C1C=1C(O)=CC=C2C=1)C(O)=O)=O)[C@H](O)C1=CC=C(C(=C1)Cl)OC=1C=C3C=C(C=1O)OC1=CC=C(C=C1Cl)C[C@H](C(=O)N1)NC([C@H](N)C=4C=C(O5)C(O)=CC=4)=O)C(=O)[C@@H]2NC(=O)[C@@H]3NC(=O)[C@@H]1C1=CC5=CC(O)=C1 DKVBOUDTNWVDEP-NJCHZNEYSA-N 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 244000046052 Phaseolus vulgaris Species 0.000 description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
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- 235000005911 diet Nutrition 0.000 description 2
- 239000000686 essence Substances 0.000 description 2
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- GOMNOOKGLZYEJT-UHFFFAOYSA-N isoflavone Chemical compound C=1OC2=CC=CC=C2C(=O)C=1C1=CC=CC=C1 GOMNOOKGLZYEJT-UHFFFAOYSA-N 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 235000013406 prebiotics Nutrition 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
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- 239000008107 starch Substances 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 230000001360 synchronised effect Effects 0.000 description 2
- 229920001285 xanthan gum Polymers 0.000 description 2
- 102000040350 B family Human genes 0.000 description 1
- 108091072128 B family Proteins 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 235000020510 functional beverage Nutrition 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 210000002429 large intestine Anatomy 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
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- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000002417 nutraceutical Substances 0.000 description 1
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- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/84—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
The invention provides a probiotics cereal beverage and powder as well as a preparation method thereof. The probiotics cereal beverage consists of the following raw materials for active ingredients in every 1000ml of water: 8 to 12 percent of cereals, 40 to 50u/ml of amylase and 100 to 120u/ml of protease, at least one probiotic is inoculated, the amount of each probiotic is 10<6> to 10<7>CFU/ml, the raw materials such as the cereals are mixed in a given ratio and then washed and crushed, the mixture is subjected to enzymolysis and then sterilized, then the probiotics are added into the mixture to be fermented, a fermented product is centrifuged, liquid supernatant is mixed in a sterile manner and canned to obtain the product, precipitation substances such as the probiotics obtained in the centrifugal process can be composited, frozen and dried in vacuum to prepare probiotics powder, the total active bacteria number in the probiotics cereal beverage can reach 10<8> to 10<9>CFU/ml, and the total bacteria number in the probiotics powder can reach 10<10> to 10<11>CFU/G.
Description
Technical field
The present invention relates to microbe application field, relate to a kind of probio cereal beverage and bacterium powder and preparation method thereof.
Background technology
Along with economic development, the improvement of living, people tend to edible more animal food, the animal food growth ratios such as the meat taken in resident's dietary structure, egg, newborn class are too high, and the absorption of the crops such as grain cereal reduces gradually, the dietary fiber that this diet structure provides is too low, energy and fat too high, cause rich man's disease and significantly rise to.Along with the progress of scientific and technological level, the use of antibiotic, hormone, various chemicals, make human body metabolism's system, enzyme system, immune system all receive impact in various degree, the microecological balance in human body is seriously damaged, thus causes modern many places in sub-health state.
In cereal preparation, carbohydrate content is generally 75%-80%, protein content 8%-10%, fat content about 1%, also containing mineral matter, B family vitamin and dietary fiber etc.Adhere to that cereal is main, effectively can keep the good tradition of China's meals, avoid the drawback of high-energy, higher fatty acid and LCD.
Probio is a class by improving intestine microenvironment thus host being produced to the microorganism of beneficial effect.It suppresses growth of pathogenic bacteria by participating in intestinal microecology balance, and avoid drug resistance and the suprainfection of the generation of Long-Time Service antibiotic, therefore, probiotic products enjoys the concern of people in recent years.
By crops deep processings such as cereal by lactobacillus-fermented exploitation probio cereal foods, can not only retain or increase nutritional labeling useful to health in these crops, and can improve mouthfeel, edible more convenient, it is easier to absorb.Application number be 201210273454.7 patent of invention disclose the production technology of a kind of synchronous solid fermentation and enzyme-squash techniqued fermented bean dregs, mainly utilize dregs of beans enzymolysis and the effect of microbiological anaerobic solid state fermentation synchronous synergetic.Although have certain requirement to the uniformity in this technique, due to when solid state fermentation, there is gradient in nutrient concentrations, ferments uneven, makes the growth of thalline, there is uneven problem to the secretion etc. of nutraceutical absorption and metabolite.And solid state fermentation production mechanization degree is lower, lack online sensor apparatus, process control is more difficult.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of probio cereal beverage and probio pulvis.
Another technical problem to be solved by this invention is the preparation method providing above-mentioned probio cereal beverage and pulvis.
For solving the problems of the technologies described above, technical scheme of the present invention is:
A kind of probio cereal beverage, in every 1000ml water, the raw material of active component consists of: cereal 8-12%, amylase 40-50u/ml, protease 100-120u/ml, inoculation at least one probio, and often kind of probio connects bacterium amount 10
6-10
7cFU/ml.
Preferably, above-mentioned probio cereal beverage, water content is 90%.
Preferably, above-mentioned probio cereal beverage, described cereal is the wherein a kind of or any combination of oat, wheat, rice, mustard wheat, brown rice, Chinese sorghum, millet, corn, glutinous rice, black rice, red bean, mung bean, soybean and black soya bean.
Preferably, above-mentioned probio cereal beverage, described cereal is made up of oat, soybean and black soya bean, counts oat 10-15 part, soybean 10-15 part, black soya bean 10-15 part by weight.
Preferably, above-mentioned probio cereal beverage, described cereal is made up of oat, soybean and black soya bean, counts oat 10.5 parts, soybean 14 parts, 10.5 parts, black soya bean by weight.
Preferably, above-mentioned probio cereal beverage, described probio is made up of Lactobacillus plantarum and animal bifidobacteria, and wherein, Lactobacillus plantarum connects bacterium amount and is: 10
6-10
7cFU/ml, animal bifidobacteria connect bacterium amount: 10
6-10
7cFU/ml.
Preferably, above-mentioned probio cereal beverage, described probio is made up of Lactobacillus plantarum and animal bifidobacteria, and wherein Lactobacillus plantarum connects bacterium amount 5-6 × 10
6cFU/ml, animal bifidobacteria connect bacterium amount 5-6 × 10
6cFU/ml.
A preparation method for probio cereal beverage, concrete steps are as follows:
(1) pulverize through beater after cereal being cleaned;
(2) amylase is added respectively after heating in feed liquid after being pulverized and protease carries out enzymolysis, wherein, amylase addition is 40-50u/ml, enzymatic hydrolysis condition is 60 DEG C-65 DEG C, 60-80min, protease addition 100-120u/ml, enzymatic hydrolysis condition are 55 DEG C-60 DEG C, 100-120min, after enzymolysis, DE value controls at 30-50%, and alpha-amido Control of Nitrogen Content is at 1.0-2.0g/100ml;
(3) feed liquid after enzymolysis is carried out sterilizing and the enzyme that goes out, wherein, described sterilising conditions is, 115 DEG C of sterilizing 30min, and go out enzyme simultaneously;
(4) after feed liquid cooling, access probio in matrix after sterilization, ferment, wherein, often kind of probio connects bacterium amount and is 10
6-10
7cFU/ml;
(5) after having fermented, undertaken centrifugal by tunning, centrifugal condition is 3000-5000r/min, 30-60min, and obtaining supernatant is probio cereal beverage; The FOS of 5%-15% (with the percentage by weight of centrifugal gained thalline and insoluble precipitate gross weight) is added as protective agent in centrifugal gained thalline and insoluble precipitate; after-70 ~-50 DEG C of pre-freeze 1-5h; carry out vacuum freeze drying; vacuum is 1-8pa; vacuum freeze drying 18-22h, makes probio pulvis.
Preferably, the preparation method of above-mentioned probio cereal beverage, in described step (4), often kind of probio connects bacterium amount is 5-6 × 10
6cFU/ml, incubation time 18h, cultivation temperature 37 ± 1 DEG C.
Preferably, the preparation method of above-mentioned probio cereal beverage, centrifugal gained supernatant can be made into probio cereal beverage, and centrifugal gained precipitation can be made into probio pulvis, achieves the maximization of raw material availability, and produces without waste liquid.
Preferably, the preparation method of above-mentioned probio cereal beverage, the obtained total viable count of probio cereal beverage can reach 10
8-10
9cFU/ml, in pulvis, total viable count can reach 10
10-10
11cFU/g.
Preferably, the preparation method of above-mentioned probio cereal beverage, centrifugal gained thalline described in step (5) and insoluble precipitate add the FOS of 10% as protective agent; Adding aseptic flavoring essence, stabilizing agent, pectin and virgin rubber in described supernatant, wherein, is 0.05-0.1% by the volume percentage flavoring essence addition of supernatant; Stabilizing agent addition is CMC 0.15-0.4%, pectin 0.15-0.3%, xanthans 0.15-0.3%.
The invention has the beneficial effects as follows:
Probio cereal beverage of the present invention, both saves the nutritive value of the crops such as cereal, has again the nutrition health-care functions of probiotics fermention goods, and mouthfeel is unique; This probio cereal beverage is prepared by specific process and obtains, the two kind large molecular nutrition composition starch maximum for content in cereal and the digestion of protein are target, amylase and protease is utilized to become to be easy to the Small molecular nutritional labeling etc. of probio and absorption of human body respectively by the starch in cereal and proteolysis, and then prepare probio cereal beverage and probio pulvis by probiotics fermention, ensureing that fermentation substrate is uniform while, be easy to realize large-scale production.
The preparation method of probio cereal beverage of the present invention, according to human nutrition absorption characteristics, the taste and particular/special requirement etc. of all ages and classes stage or different crowd, select the crops such as specific foodstuff grain, clean and crush after the mixing according to a certain percentage such as cereal, sterilizing is carried out after enzymolysis, access probio after sterilizing to ferment, product after fermentation is carried out centrifugal, supernatant through aseptic allotment, cannedly namely obtain product, and the deposits such as centrifugal obtained probio can make probio pulvis through composite, vacuum freeze drying.This have very large market development potential by insoluble substances such as the bacterial sediment of centrifugal for fermentation collected acquisition and strings through being processed into the technique being rich in probio pulvis, and concrete outstanding effect is as follows:
(1) raw material range of choice is extensive, can according to the trophic function feature of the crops such as various foodstuff grains, make full use of the characteristic of enzyme and probio, the macromolecular substances in raw material is resolved into the small-molecule substance being easier to absorption of human body, be also more conducive to growth of microorganism;
(2) general probio pulvis raw materials for production cost is higher, can produce a large amount of waste liquid in process of production, cause wastage of material and environmental pollution; With crops such as foodstuff grains for raw material, greatly reduce production cost; By manufacture craft of the present invention, functional beverage made by supernatant, and solids of sedimentation then makes probio pulvis, achieves the maximization of raw material availability, and produces without waste liquid;
(3) except containing except a large number of nutrients in the probio cereal beverage obtained by this preparation method, the total viable count of the probio contained reaches 10
8-10
9cFU/ml, in gained pulvis, the total viable count of probio reaches 10
10-10
11cFU/g.
Accompanying drawing explanation
Fig. 1: cereal adding proportion is on the impact of viable count total in gained probio cereal beverage after fermentation and probio pulvis;
Fig. 2: inoculum concentration is on the impact of viable count total in gained probio cereal beverage after fermentation and probio pulvis;
Fig. 3: incubation time is on the impact of viable count total in gained probio cereal beverage after fermentation and probio pulvis.
Detailed description of the invention
Below in conjunction with specific embodiment, technical scheme of the present invention is further described.
Embodiment 1
A kind of probio cereal beverage and pulvis, in every 1000ml water, the raw material of active component consists of: cereal 10%, amylase 45u/ml, protease 110u/ml, inoculation probio: Lactobacillus plantarum (CGMCC1.6971, CGMCC: China General Microbiological culture presevation administrative center) and animal bifidobacteria (CGMCC 1.3003), wherein Lactobacillus plantarum 6 × 10
6cFU/ml, animal bifidobacteria 6 × 10
6cFU/ml, described cereal is made up of oat, soybean and black soya bean, counts oat 10.5 parts, soybean 14 parts, 10.5 parts, black soya bean by weight.
The preparation method of above-mentioned probio cereal beverage and pulvis, concrete steps are as follows:
(1) pulverize through beater after cereal being cleaned;
(2) amylase is added respectively after heating in feed liquid after being pulverized and protease carries out enzymolysis, wherein, amylase addition is 45u/ml, enzymatic hydrolysis condition is 63 DEG C, 70min, protease addition 110u/ml, enzymatic hydrolysis condition are 57 DEG C, 110min, after enzymolysis, DE value controls 32%, and alpha-amido Control of Nitrogen Content is at 1.2g/100ml;
(3) feed liquid after enzymolysis is carried out sterilizing and the enzyme that goes out, wherein, described sterilising conditions is, 115 DEG C of sterilizing 30min, and go out enzyme simultaneously;
(4) after feed liquid cooling, access probio Lactobacillus plantarum in matrix after sterilization and animal bifidobacteria ferments, wherein, it is 6 × 10 that often kind of probio connects bacterium amount
6cFU/ml, incubation time 18h, cultivation temperature 37 ± 1 DEG C;
(5) after having fermented, undertaken centrifugal by tunning, centrifugal condition is 4000r/min, 45min, and obtaining supernatant is probio cereal beverage; Centrifugal gained thalline and insoluble precipitate are added the FOS of 10% as protective agent, and after-60 DEG C of pre-freeze 3h, carry out vacuum freeze drying, vacuum is 5pa, vacuum freeze drying 20h, makes probio pulvis.
Total viable count in gained probio cereal beverage is 7.35 × 10
8cFU/ml.Wherein, Lactobacillus plantarum viable count 6.38 × 10
8cFU/ml, it is 9.70 × 10 that the viable count of animal bifidobacteria can reach
7cFU/ml.Total viable count in probio pulvis is 7.21 × 10
10cFU/g.Wherein Lactobacillus plantarum viable count 6.47 × 10
10it is 7.40 × 10 that the viable count of CFU/g and animal bifidobacteria can reach
9cFU/g.
Embodiment 2
A kind of probio cereal beverage and pulvis, in every 1000ml water, the raw material of active component consists of: cereal 8%, amylase 50u/ml, protease 100u/ml, inoculation probio: Lactobacillus plantarum (CGMCC1.3921) and animal bifidobacteria (CGMCC 1.1852), wherein Lactobacillus plantarum 10
6cFU/ml, animal bifidobacteria 10
6cFU/ml, described cereal is made up of oat, soybean and black soya bean, counts oat 15 parts, soybean 10 parts, 15 parts, black soya bean by weight.
The preparation method of above-mentioned probio cereal beverage and pulvis, concrete steps are as follows:
(1) pulverize through beater after cereal being cleaned;
(2) amylase is added respectively after heating in feed liquid after being pulverized and protease carries out enzymolysis, wherein, amylase addition is 50u/ml, enzymatic hydrolysis condition is 60 DEG C, 80min, protease addition 100u/ml, enzymatic hydrolysis condition are 60 DEG C, 100min, after enzymolysis, DE value controls 30%, and alpha-amido Control of Nitrogen Content is at 2.0g/100ml;
(3) feed liquid after enzymolysis is carried out sterilizing and the enzyme that goes out, wherein, described sterilising conditions is, 115 DEG C of sterilizing 30min, and go out enzyme simultaneously;
(4) after feed liquid cooling, access probio Lactobacillus plantarum in matrix after sterilization and animal bifidobacteria ferments, wherein, it is 10 that often kind of probio connects bacterium amount
6cFU/ml, incubation time 14h, cultivation temperature 37 ± 1 DEG C;
(5) after having fermented, undertaken centrifugal by tunning, centrifugal condition is 3000r/min, 60min, and obtaining supernatant is probio cereal beverage; Centrifugal gained thalline and insoluble precipitate are added the FOS of 5% as protective agent, and after-70 DEG C of pre-freeze 1h, carry out vacuum freeze drying, vacuum is 8pa, vacuum freeze drying 18h, makes probio pulvis.
Total viable count in gained probio cereal beverage is 6.69 × 10
8cFU/ml.Wherein, Lactobacillus plantarum viable count 6.10 × 10
8cFU/ml, it is 5.90 × 10 that the viable count of animal bifidobacteria can reach
7cFU/ml.Total viable count in probio pulvis is 6.20 × 10
10cFU/g.Wherein Lactobacillus plantarum viable count 5.61 × 10
10it is 5.50 × 10 that the viable count of CFU/g and animal bifidobacteria can reach
9cFU/g.
Embodiment 3
A kind of probio cereal beverage and pulvis, in every 1000ml water, the raw material of active component consists of: cereal 12%, amylase 40u/ml, protease 120u/ml, inoculation probio: Lactobacillus plantarum (CGMCC1.3915) and animal bifidobacteria (CGMCC 1.1852), wherein Lactobacillus plantarum 10
7cFU/ml, animal bifidobacteria 10
7cFU/ml, described cereal is made up of oat, soybean and black soya bean, counts oat 12 parts, soybean 13 parts, 11 parts, black soya bean by weight.
The preparation method of above-mentioned probio cereal beverage and pulvis, concrete steps are as follows:
(1) pulverize through beater after cereal being cleaned;
(2) amylase is added respectively after heating in feed liquid after being pulverized and protease carries out enzymolysis, wherein, amylase addition is 40u/ml, enzymatic hydrolysis condition is 65 DEG C, 60min, protease addition 120u/ml, enzymatic hydrolysis condition are 55 DEG C, 120min, after enzymolysis, DE value controls 50%, and alpha-amido Control of Nitrogen Content is at 1.0g/100ml;
(3) feed liquid after enzymolysis is carried out sterilizing and the enzyme that goes out, wherein, described sterilising conditions is, 115 DEG C of sterilizing 30min, and go out enzyme simultaneously;
(4) after feed liquid cooling, access probio Lactobacillus plantarum in matrix after sterilization and animal bifidobacteria ferments, wherein, it is 10 that often kind of probio connects bacterium amount
7cFU/ml, incubation time 26h, cultivation temperature 37 ± 1 DEG C;
(5) after having fermented, undertaken centrifugal by tunning, centrifugal condition is 5000r/min, 30min, and obtaining supernatant is probio cereal beverage; Centrifugal gained thalline and insoluble precipitate are added the FOS of 15% as protective agent, and after-50 DEG C of pre-freeze 5h, carry out vacuum freeze drying, vacuum is 1pa, vacuum freeze drying 22h, makes probio pulvis.
Total viable count in gained probio cereal beverage is 6.53 × 10
8cFU/ml.Wherein, Lactobacillus plantarum viable count 6.01 × 10
8cFU/ml, it is 4.20 × 10 that the viable count of animal bifidobacteria can reach
7cFU/ml.Total viable count in probio pulvis is 6.73 × 10
10cFU/g.Wherein Lactobacillus plantarum viable count 6.08 × 10
10it is 6.50 × 10 that the viable count of CFU/g and animal bifidobacteria can reach
9cFU/g.
Embodiment 4
A kind of probio cereal beverage and pulvis, in every 1000ml water, the raw material of active component consists of: cereal 11%, amylase 47u/ml, protease 115u/ml, inoculation probio: Lactobacillus plantarum (CGMCC1.3915) and animal bifidobacteria (CGMCC 1.2226), wherein Lactobacillus plantarum 5 × 10
6cFU/ml, animal bifidobacteria 5 × 10
6cFU/ml, described cereal is mixed by weight ratios such as wheat, rice, mustard wheat, brown rice, Chinese sorghum, millet, corn, glutinous rice, black rice, red bean and mung beans and forms.
The preparation method of above-mentioned probio cereal beverage and pulvis, concrete steps are as follows:
(1) pulverize through beater after cereal being cleaned;
(2) amylase is added respectively after heating in feed liquid after being pulverized and protease carries out enzymolysis, wherein, amylase addition is 47u/ml, enzymatic hydrolysis condition is 62 DEG C, 75min, protease addition 115u/ml, enzymatic hydrolysis condition are 58 DEG C, 115min, after enzymolysis, DE value controls 35%, and alpha-amido Control of Nitrogen Content is at 1.5g/100ml;
(3) feed liquid after enzymolysis is carried out sterilizing and the enzyme that goes out, wherein, described sterilising conditions is, 115 DEG C of sterilizing 30min, and go out enzyme simultaneously;
(4) after feed liquid cooling, access probio Lactobacillus plantarum in matrix after sterilization and animal bifidobacteria ferments, wherein, it is 5 × 10 that often kind of probio connects bacterium amount
6cFU/ml, incubation time 22h, cultivation temperature 37 ± 1 DEG C;
(5) after having fermented, undertaken centrifugal by tunning, centrifugal condition is 4000r/min, 45min, and obtaining supernatant is probio cereal beverage; Centrifugal gained thalline and insoluble precipitate are added the FOS of 15% as protective agent, and after-60 DEG C of pre-freeze 4h, carry out vacuum freeze drying, vacuum is 4pa, vacuum freeze drying 20h, makes probio pulvis.
Total viable count in gained probio cereal beverage is 6.51 × 10
8cFU/ml.Wherein, Lactobacillus plantarum viable count 6.02 × 10
8cFU/ml, it is 4.90 × 10 that the viable count of animal bifidobacteria can reach
8cFU/ml.Total viable count in probio pulvis is 6.35 × 10
10cFU/g.Wherein Lactobacillus plantarum viable count 5.87 × 10
10it is 4.80 × 10 that the viable count of CFU/g and animal bifidobacteria can reach
9cFU/g.
In above-described embodiment 1-4, gained supernatant (probio cereal beverage) can add aseptic flavoring essence, stabilizing agent, pectin and virgin rubber according to needs of production, wherein, be 0.05-0.1% by the volume percentage flavoring essence addition of supernatant; Stabilizing agent addition is CMC 0.15-0.4%, pectin 0.15-0.3%, xanthans 0.15-0.3%.
Embodiment 5 screens example
The determination of screening example 1 cereal materials proportioning
After being processed by raw material as stated above, in institute's dosing state fermentation substrate, water content is 90%, oat: soybean: black soya bean ratio is respectively (%): 2:3:5; 3:3:4; 3:4:3; 4:3:3; 4:4:2; 115 DEG C of sterilizing 30min.
Connect bacterium amount and condition of culture: access Lactobacillus plantarum (CGMCC 1.6971) and animal bifidobacteria (CGMCC 1.3003), the inoculum concentration of two strains of lactic acid bacteria is 5 × 10 simultaneously
6cFU/ml, pH nature, cultivation temperature 37 ± 1 DEG C, cultivates 20h.The total viable count of probio measured in the rear gained probio cereal beverage of fermentation and probio pulvis carries out.
Result is as shown in Figure 1: best formula of cultivating is for (%): oat: soybean: black soya bean ratio is 3:4:3.Namely the total viable count recorded in gained probio cereal beverage is 3.21 × 10
8cFU/ml.Wherein, Lactobacillus plantarum viable count 2.72 × 10
8cFU/ml, it is 4.9 × 10 that the viable count of animal bifidobacteria can reach
7cFU/ml.Total viable count in probio pulvis is 4.70 × 10
10cFU/g.Wherein Lactobacillus plantarum viable count 4.42 × 10
10it is 4.8 × 10 that the viable count of CFU/g and animal bifidobacteria can reach
9cFU/g.
The determination of screening example 2 inoculum concentration
Ferment by the different bacterium amount that connects Simultaneous vaccination Lactobacillus plantarum (CGMCC 1.6971) and animal bifidobacteria (CGMCC 1.3003) in the oat optimized in cereal proportioning, soybean, black soya bean mixes liquid matrix.
Connect bacterium amount and condition of culture: the bacterium amount that connects of two strains of lactic acid bacteria is respectively: 2 × 10
6cFU/ml; 4 × 10
6cFU/ml; 6 × 10
6cFU/ml; 8 × 10
6cFU/ml.PH nature, cultivation temperature 37 ± 1 DEG C, cultivates 20h altogether, detects the total viable count of probio in gained probio cereal beverage after fermentation and probio pulvis.
Result is as shown in Figure 2: it is 6 × 10 that the best connects bacterium amount
6cFU/ml, the total viable count namely recorded in gained probio cereal beverage is 5.61 × 10
8cFU/ml.Wherein, Lactobacillus plantarum viable count 4.92 × 10
8cFU/ml, it is 6.9 × 10 that the viable count of animal bifidobacteria can reach
7cFU/ml.Total viable count in probio pulvis is 7.1 × 10
10cFU/g.Wherein Lactobacillus plantarum viable count is 6.70 × 10
10cFU/g, animal bifidobacteria viable count is 4.14 × 10
9cFU/g.
The determination of screening example 3 incubation time
Access inoculation plant breast bar (CGMCC 1.6971) and animal bifidobacteria (CGMCC 1.3003) simultaneously in the oat optimized in cereal proportioning, soybean, black soya bean liquid state fermentation matrix.
Connect bacterium amount and incubation time: the bacterium amount that connects of two strains of lactic acid bacteria is 6 × 10 respectively
6cFU/ml, pH nature, cultivation temperature 37 ± 1 DEG C, ferment 14 respectively, 18,22,26h, the number of live bacteria of probiotics in gained probio cereal beverage after fermentation and probio pulvis is detected.
Result is as shown in Figure 3: best incubation time is 18h.Namely the total viable count recorded in gained probio cereal beverage is 7.85 × 10
8cFU/ml.Wherein, Lactobacillus plantarum viable count 7.02 × 10
8it is 8.3 × 10 that the viable count of CFU/ml and animal bifidobacteria can reach
7cFU/ml.Total viable count in gained probio pulvis is 7.67 × 10
10cFU/g.Wherein Lactobacillus plantarum viable count is 6.81 × 10
10cFU/g, animal bifidobacteria viable count are 8.61 × 10
9cFU/g.
Embodiment 6 determination test example
Determination test example 1
The mensuration of total dietary fiber, soluble dietary fiber, insoluble diedairy fiber, beta glucan, isoflavones and amino-acid nitrogen content in probio cereal beverage before and after single bacterium, mixed bacterium enzymatic hydrolysis and fermentation
AACC32-07 and AOAC995.16 method is utilized to carry out measurement result in table 1 to total dietary fiber, soluble dietary fiber, insoluble dietary fiber content and the beta glucan content before single bacterium or mixed bacterium enzymatic hydrolysis and fermentation and after fermentation in gained probio cereal beverage.Utilize GB/T 23788-2009 method, the isoflavone content before single bacterium or mixed bacterium enzymatic hydrolysis and fermentation and after fermentation in gained probio cereal beverage is measured, the results are shown in Table 2.Utilize GB/T12143.2-89, the content of the amino nitrogen before measuring single bacterium or mixed bacterium enzymatic hydrolysis and fermentation and after fermentation in gained probio cereal beverage, the results are shown in Table 3.Wherein, as the beta glucan content of important prebiotics before enzymolysis and after fermentation in gained probio cereal beverage content be more or less the same, in a slight decrease.Isoflavones exists with glucosides and aglycon two kinds of forms.In probio cereal beverage, the Aglycones content be more easily absorbed by the body increases.Protein is under the effect of enzyme and probio, and amino nitrogen level increases to some extent.
Determination test example 2
The mensuration of total dietary fiber, soluble dietary fiber, insoluble diedairy fiber, beta glucan, isoflavones and amino-acid nitrogen content in probio pulvis before and after single bacterium, mixed bacterium enzymatic hydrolysis and fermentation
AACC32-07 and AOAC995.16 method is utilized to carry out measurement result in table 4 to total dietary fiber, soluble dietary fiber, insoluble dietary fiber content and the beta glucan content before enzymatic hydrolysis and fermentation and after fermentation in gained probio pulvis.Utilize GB/T 23788-2009 method, the isoflavone content before single bacterium or mixed fungus fermentation and after fermentation in gained probio pulvis is measured, the results are shown in Table 5.Utilize GB/T12143.2-89, the content of the amino nitrogen before measuring single bacterium or mixed fungus fermentation and after fermentation in gained probio pulvis, the results are shown in Table 6.Wherein, as important prebiotics beta glucan content reduce more, mainly due to centrifugal or filter after gained precipitation in, containing more insoluble diedairy fiber, soluble dietary fibre content is little.In probio pulvis, the Aglycones content be more easily absorbed by the body increases.Protein is under the effect of enzyme and probio, and amino nitrogen level increases to some extent.
Contain highly active two kinds of probios in the gained of instantiation described in invention probio cereal beverage and probio pulvis all simultaneously.Wherein in probio cereal beverage, Lactobacillus plantarum viable count is no less than 6.93 × 10
8cFU/ml, the viable count of animal bifidobacteria is no less than 8.10 × 10
7cFU/ml; In probio pulvis, Lactobacillus plantarum viable count is no less than 6.03 × 10
10cFU/g, the viable count of animal bifidobacteria is no less than 5.65 × 10
9cFU/g.Except containing except abundant nutriment in product of the present invention, also containing a large amount of probio.Lactobacillus plantarum mainly acts on the small intestine of human body, and animal bifidobacteria mainly acts on the large intestine of human body, thus better nurses one's health intestine microenvironment, and then promotes health.
The changes of contents of dietary fiber and beta glucan in gained probio cereal beverage before the single bacterium of table 1 or mixed bacterium enzymatic hydrolysis and fermentation and after fermentation
The changes of contents of glucosides and aglycon in gained probio cereal beverage before the single bacterium of table 2 or mixed bacterium enzymatic hydrolysis and fermentation and after fermentation
The changes of contents of amino nitrogen in gained probio cereal beverage before the single bacterium of table 3 or mixed bacterium enzymatic hydrolysis and fermentation and after fermentation
The changes of contents of dietary fiber and beta glucan in gained probio pulvis before the single bacterium of table 4 or mixed bacterium enzymatic hydrolysis and fermentation and after fermentation
The changes of contents of glucosides and aglycon in gained probio pulvis before the single bacterium of table 5 or mixed bacterium enzymatic hydrolysis and fermentation and after fermentation
The changes of contents of amino nitrogen in gained probio pulvis before the single bacterium of table 6 or mixed bacterium enzymatic hydrolysis and fermentation and after fermentation
In summary it can be seen:
1, after enzymolysis process in gained probio cereal beverage and probio pulvis total viable count higher than the viable count in the sample without enzymolysis process gained.And the content of the nutriment such as beta glucan contained in the probio cereal beverage of enzymolysis and mixed fungus fermentation two process gained and amino nitrogen is all higher than the beverage only having enzymolysis or the single process gained of mixed fungus fermentation.
2, freeze drying protectant can make the active material of biological products from destruction in freeze-drying process or after freeze-drying process, thus improves survival rate in preservation process of the survival rate of bioactivator in freeze-drying process and freeze-dried products and storage life.The condition of vacuum freeze drying described in screening example is through optimization and draws, adding 10% FOS is protective agent, and in the probio pulvis of gained, total viable count is the highest, and storage life is the longest.
Above-mentioned detailed description of this kind of probio cereal beverage and preparation method thereof being carried out with reference to embodiment; illustrative instead of determinate; several embodiments can be listed according to institute's limited range; therefore in the change do not departed under general plotting of the present invention and amendment, should belong within protection scope of the present invention.
Claims (10)
1. a probio cereal beverage, is characterized in that: in every 1000ml water, the raw material of active component consists of: cereal 8-12%, amylase 40-50u/ml, protease 100-120u/ml, inoculation at least one probio, and often kind of probio 10
6-10
7cFU/ml.
2. probio cereal beverage according to claim 1, is characterized in that: water content is 90%.
3. probio cereal beverage according to claim 1, is characterized in that: described cereal is the wherein a kind of or any combination of oat, wheat, rice, mustard wheat, brown rice, Chinese sorghum, millet, corn, glutinous rice, black rice, red bean, mung bean, soybean and black soya bean.
4. the probio cereal beverage according to claim 1 or 3, is characterized in that: described cereal is made up of oat, soybean and black soya bean, counts oat 10-15 part, soybean 10-15 part, black soya bean 10-15 part by weight.
5. probio cereal beverage according to claim 4, is characterized in that: described cereal is made up of oat, soybean and black soya bean, counts oat 10.5 parts, soybean 14 parts, 10.5 parts, black soya bean by weight.
6. probio cereal beverage according to claim 1, is characterized in that: described probio is made up of Lactobacillus plantarum and animal bifidobacteria, wherein connects bacterium amount and is: Lactobacillus plantarum 10
6-10
7cFU/ml, animal bifidobacteria 10
6-10
7cFU/ml.
7. probio cereal beverage according to claim 6, is characterized in that: described probio is made up of Lactobacillus plantarum and animal bifidobacteria, wherein connects bacterium amount and is: Lactobacillus plantarum 5-6 × 10
6cFU/ml, animal bifidobacteria 5-6 × 10
6cFU/ml.
8. the preparation method of probio cereal beverage described in claim 1, is characterized in that: concrete steps are as follows:
(1) pulverize through beater after cereal being cleaned;
(2) amylase is added respectively after heating in feed liquid after being pulverized and protease carries out enzymolysis, wherein, amylase addition is 40-50u/ml, enzymatic hydrolysis condition is 60 DEG C-65 DEG C, 60-80min, protease addition 100-120u/ml, enzymatic hydrolysis condition are 55 DEG C-60 DEG C, 100-120min, after enzymolysis, DE value controls at 30-50%, and alpha-amido Control of Nitrogen Content is at 1.0-2.0g/100ml;
(3) feed liquid after enzymolysis is carried out sterilizing and the enzyme that goes out, wherein, described sterilising conditions is, 115 DEG C of sterilizing 30min, and go out enzyme simultaneously;
(4) after feed liquid cooling, access probio in matrix after sterilization, ferment, wherein, it is 10 that often kind of probio connects bacterium amount
6-10
7cFU/ml;
(5) after having fermented, undertaken centrifugal by tunning, centrifugal condition is 3000-5000r/min, 30-60min, and obtaining supernatant is probio cereal beverage; Centrifugal gained thalline and insoluble precipitate are added the FOS of 5%-15% as protective agent, and after-70 ~-50 DEG C of pre-freeze 1-5h, carry out vacuum freeze drying, vacuum is 1-8pa, vacuum freeze drying 18-22h, makes probio pulvis.
9. the preparation method of probio cereal beverage according to claim 8, is characterized in that: in described step (4), often kind of probio connects bacterium amount is 5-6 × 10
6cFU/ml, incubation time 18h, cultivation temperature 37 ± 1 DEG C.
10. the preparation method of probio cereal beverage according to claim 8, is characterized in that: centrifugal gained thalline described in step (5) and insoluble precipitate add the FOS of 10% as protective agent.
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