CN105942061A - Method of preparing water chestnut juice through composite enzymolysis - Google Patents
Method of preparing water chestnut juice through composite enzymolysis Download PDFInfo
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- CN105942061A CN105942061A CN201610347596.1A CN201610347596A CN105942061A CN 105942061 A CN105942061 A CN 105942061A CN 201610347596 A CN201610347596 A CN 201610347596A CN 105942061 A CN105942061 A CN 105942061A
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- 239000002131 composite material Substances 0.000 title claims abstract description 6
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- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 12
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/84—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Polymers & Plastics (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Non-Alcoholic Beverages (AREA)
- Enzymes And Modification Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a method of preparing water chestnut juice through composite enzymolysis and belongs to the field of food technology and bioengineering. The method includes: pulping water chestnut; adopting a twice-enzymolysis mode. A composite enzyme preparation composed of cellulase, pectinase, amylase, glucoamylase and protease is used to decompose water chestnut tissue, synergistic effect of a composite enzyme is brought into full play, soluble micromolecular substances in the water chestnut tissue are released to greatest extent, and juicing rate and nutritional substance content of the water chestnut juice are increased.
Description
Technical field
The present invention relates to a kind of method that complex enzyme hydrolysis prepares Horse hoof fruit juice, belong to food technology and bioengineering field.
Background technology
Horse hoof (Chines water chestnut), formal name used at school Corm Eleocharitis (Eleochaurus toberosa), for the underground ball of Cyperaceae Eleocharis
Stem, rich in carbohydrate, polysaccharide, various vitamin, carotene, fat, protein, trace element, Huang in fruit
The nutrient substance such as ketone and Polyphenols.Research shows, Horse hoof has regulation blood fat, regulates the multiple lifes such as immune, antibacterial antioxidation
Reason function, has good medical value, is excellent health food.
In carrying out Horse hoof fruit juice preparation process, owing to Horse hoof contains abundant starchy material, and traditional crushing juice rate of squeezing the juice
Relatively low, therefore direct expression should not use in extracting Horse hoof fruit juice.Also glucoamylase enzyme solution is utilized to replace tradition to squeeze the juice method enzyme
Solving Horse hoof, discovery can improve its crushing juice rate, but single enzyme solution effect is the most undesirable.
Enzyme preparation, as a class green safety food additive, has been got in the application improved on food quality and food making process
Come the most universal.Along with biotechnology is in progress, the enzyme preparation that constantly research, exploitation make new advances, it has also become the newest raw-food material is opened
Send out, the important step of quality-improving, technological transformation.
First, Food enzyme is that the source bacterial strain meeting the requirement of food additive GB2760 is according to food additive microlitre mark
The biological enzyme formulation as food processing aid that alignment request and enzyme preparation production environment, equipment requirements produce.Enzyme comes from nature
In life entity, such as microorganism, plant, animal, itself has safety；Secondly, Food enzyme is only in the course of processing
Playing catalytic chemistry response function, enzyme will not remain in food；3rd, the reaction condition of enzyme is gentle, has specificity height, urges
Change speed fast, low to nutrient substance damage, the feature of no coupling product generation, it is ensured that the nutrition of food.As can be seen here, exist
It is the safest for applying enzyme preparation in food processing process, can substitute those harmful materials, or harmful
Material is converted into harmless, reduces insecure factors of food, it addition, enzyme preparation also have shortening production process, consumption few, green,
The advantage such as safe efficient.
According to retrieval, existing Horse hoof converted products, the product etc. of brewageing including fruit juice and correspondence is studied less, mainly due to horse
Hoof is rich in much starch class material, and the factors such as Horse hoof crushing juice rate is relatively low, product is unstable limit follow-up Horse hoof deep processing application
Research.In consideration of it, develop a kind of complex enzyme hydrolysis to prepare the technical study method of Horse hoof fruit juice, it is used for increasing substantially Horse hoof juice
Rate, and avoid precipitation or the muddiness caused due to pectin, starch etc., final acquisition has good stability, the fruit juice that mouthfeel is suitable
Product is necessary.
Summary of the invention
In place of it is an object of the invention to overcome the deficiencies in the prior art, it is provided that a kind of enzymolysis is complete, juice yield is high, product
Stable, the compound enzymic preparation that mouthfeel is suitable prepares the technical study method of Horse hoof fruit juice.
The complex enzyme hydrolysis of the present invention prepares the method for Horse hoof fruit juice, includes the following:
(A) Horse hoof is cleaned (peeling), steaming and decocting, pulverize, add water and size mixing；
(B) enzymolysis: add water and adjust the concentration of serosity, regulation pH value, to 4.0-6.0, adds cellulase, pectase, starch
Enzyme, saccharifying enzyme, protease, cellulase dosage is 8000-48000U/kg (for Horse hoof raw material, lower with), really
Glue enzyme dosage is 8000-48000U/kg, amylase dosage is 8000-48000U/kg, saccharifying enzyme dosage is 8000-48000U/kg,
Protease dosage is 5000-40000U/kg, hydrolysis temperature 30-60 DEG C, and enzymolysis time is 0.5-4h；
(C) enzyme denaturing is boiled after enzymolysis, cooling, obtain turbid juice, or add the adsorbent suction that enzymolysis solution percentage by weight is 0.5%-1.5%
Attached, filter, make clear juice.
In one embodiment of the invention, in described enzymolysis process, cellulase, pectase, amylase, saccharifying enzyme,
The enzyme of protease lives ratio for (4～6): (4～6): (2～6): (2～4): 1.
In one embodiment of the invention, the enzymolysis of described step (B) is carried out at twice；Wherein
Enzymolysis for the first time: add water and adjust the concentration of serosity so that Horse hoof raw material is 1:2-1:6 with the ratio of water, regulation pH value is extremely
4.0-6.0, adds compound enzyme I, enzymolysis time 30-60min, hydrolysis temperature 30-40 DEG C；Compound enzyme I is lived ratio for (4.5～5.5) by enzyme:
(4.5～5.5): (2～4): the cellulase of (2.5～3.5), pectase, amylase, saccharifying enzyme form, cellulase dosage
For 8000-48000U/kg, pectase dosage be 8000-48000U/kg, amylase dosage be 8000-48000U/kg, saccharifying
Enzyme dosage is 8000-48000U/kg.
Enzymolysis for the second time: add water and adjust the concentration of enzymolysis solution for the first time so that the enzymolysis obtained after Horse hoof raw material and for the first time enzymolysis
Liquor ratio value is 1:4-1:6, regulates pH, makes pH value at pH 4.0-6.0, adds compound enzyme II, under the conditions of 50-60 DEG C, enzymolysis
0.5-3 hour, obtain second time enzymolysis solution；Described compound enzyme II is made up of amylase, saccharifying enzyme, protease；Wherein amylase,
Saccharifying enzyme, the enzyme of protease are lived than for (1～2): (1～2): (1～2), wherein amylase dosage be 8000-48000U/kg,
Saccharifying enzyme dosage is 8000-48000U/kg, protease dosage is 5000-40000U/kg.
In one embodiment of the invention, described compound enzyme I is the cellulase of 5:5:3:2, pectase, shallow lake by enzyme ratio alive
Powder enzyme, saccharifying enzyme form.
In one embodiment of the invention, described compound enzyme II is the amylase of 1:1:1, saccharifying enzyme, egg by enzyme ratio alive
White enzyme composition.
In one embodiment of the invention, described first time enzymolysis is to carry out under pH 4.0-5.0.
In one embodiment of the invention, described method is specific as follows:
(1) Horse hoof is cleaned, steaming and decocting, pulverize, add water and size mixing；
(2) enzymolysis: add water and adjust the concentration of serosity so that Horse hoof raw material is 1:2-1:6 with the ratio of water, regulates pH value, makes
PH refers at 4.0-6.0, adds compound enzyme I, cellulase preparation: pectase preparation: diastase: saccharidase preparation enzyme is lived
Ratio is 5:5:3:2, enzymolysis time 30-60min, hydrolysis temperature 30-40 DEG C, enzymolysis pH4.0-5.0, and cellulase dosage is
8000-48000U/kg (for Horse hoof raw material, lower same), pectase dosage are 8000-48000U/kg, amylase adds
Amount is 8000-48000U/kg for 8000-48000U/kg, saccharifying enzyme dosage；
(3) add water and adjust the concentration of for the first time enzymolysis solution so that Horse hoof raw material and enzymolysis solution ratio are 1:4-1:6, regulate pH,
Making pH value at pH 4.0-6.0, add compound enzyme II (amylase, saccharifying enzyme, protease), amylase dosage is 8000-48000
U/kg, saccharifying enzyme dosage are 8000-48000U/kg, protease dosage is 5000-40000U/kg, under the conditions of 50-60 DEG C,
Enzymolysis 0.5-3 hour, obtain second time enzymolysis solution, the total crushing juice rate of Horse hoof be 80-90% (gross weight of crushing juice rate=juice of squeezing out/
Obtain weight * 100% that juice is processed)；
(4) secondary enzymolysis liquid is boiled enzyme denaturing, cooling, obtain turbid juice, or to add enzymolysis solution percentage by weight be 0.5%-1.5% absorption
Agent is adsorbed, and filters, makes clear juice.
In one embodiment of the invention, the pH adjusting agent used during described regulation pH value is food stage citric acid or dilute
Hydrochloric acid.
In one embodiment of the invention, described adsorbent is gauze (4-8 layer) or activated carbon or silica gel or molecular sieve etc..
Beneficial effects of the present invention:
1, the present invention uses acidity and neutral enzymatic hydrolysis Horse hoof, without adding alkali in whole course of reaction, retains the original spy of fruit juice
Property.
2, the present invention utilizes compound enzymic preparation by Horse hoof tissue breakdown under the conditions of relatively mild, adds the release of effective ingredient, from
And improve the crushing juice rate of Horse hoof fruit juice.
3, cellulase, pectase, amylase, saccharifying enzyme, the compound enzymic preparation of protease composition are used for decomposing horse by the present invention
Hoof tissue, has removed the compositions such as the pectin on cell wall and cell membrane, cellulose and protein so that cell crushes smoothly, point
Solution becomes solubility small-molecule substance, reaches to improve crushing juice rate, reduce energy consumption and effectively protect the purpose of fruit juice primary pulp nutritional labeling.
4, the present invention utilizes composite biological enzyme preparation to prepare Horse hoof fruit juice, can give full play to the synergy of compound enzyme, to greatest extent
The solubility small-molecule substance in Horse hoof tissue is discharged, improve Horse hoof juice yield and nutritional labeling thereof.
Accompanying drawing explanation
Fig. 1 is the process chart of the embodiment of the present invention.
Detailed description of the invention
Being described further the technology contents of the present invention below in conjunction with embodiment, following embodiment is illustrative, is not to limit
Property, it is impossible to limit protection scope of the present invention with following embodiment.
Cellulase described in following example, pectase, amylase, saccharifying enzyme, protease are commercially available prod.
Embodiment 1
A kind of complex enzyme hydrolysis Horse hoof fruit juice technical study method, step is as follows:
(1) 1kg Horse hoof is cleaned, steaming and decocting 15-20 minute, after softening, pulverize, according to the part by weight 1:2 of Horse hoof Yu water
Add water and size mixing.
(2) enzymolysis for the first time: adding water and adjust the concentration of serosity, making the weight of Horse hoof and the ratio of water is 1:2, then uses food stage
Citric acid or dilute hydrochloric acid regulate its pH value, make pH at 4.0-5.0, are subsequently adding compound enzyme I, enzymolysis time 30-45min, enzyme
Solving temperature 35-40 DEG C, temperature regulation can use water-bath heater, and enzymolysis is carried out under low rate mixing state, obtains enzymolysis solution.
Wherein, compound enzyme I is made up of cellulase, pectase, amylase, saccharifying enzyme, and wherein enzyme lives ratio respectively
2.5:2.5:1.5:1, cellulase enzyme concentration is 25000U/kg Horse hoof raw material), pectase 25000U/kg (Horse hoof raw material),
Amylase 15000U/kg (Horse hoof raw material), saccharifying enzyme 10000U/kg (Horse hoof raw material).
(3) enzymolysis for the second time: adding water and adjust the concentration of enzymolysis solution for the first time, making the weight of Horse hoof and the ratio of enzymolysis solution is 1:5,
Use food stage citric acid or dilute hydrochloric acid to regulate its pH value again, make pH 4.5, be subsequently adding compound enzyme II, 55 DEG C of conditions
Under, enzymolysis 1h, temperature regulation can use water-bath heater, and enzymolysis is carried out under low rate mixing state, obtains enzymolysis solution.
Wherein, compound enzyme II is made up of amylase, saccharifying enzyme, protease, and wherein ratio is respectively 1:1:1, amylase enzyme concentration
For 8000U/kg Horse hoof raw material), saccharifying enzyme 8000U/kg (Horse hoof raw material), protease 5000U/kg (Horse hoof raw material).
(4) second time enzymolysis solution is boiled 18 minutes enzyme denaturing, cooling, obtain turbid juice, or to add percentage by weight be that 1% adsorbent is inhaled
Attached, filter, make clear juice.
When preparing clear juice, according to weight * 100% that the gross weight/acquisition juice of crushing juice rate=juice of squeezing out is processed, record enzymolysis
Rear crushing juice rate is up to 89%, and compared with the result of contrast test 1, crushing juice rate significantly improves.
Embodiment 2
A kind of method of complex enzyme hydrolysis Horse hoof fruit juice, step is as follows:
(1) 2kg Horse hoof is cleaned, steaming and decocting 30 minutes, after softening, pulverize, add according to the part by weight 1:2 of Horse hoof with water
Water is sized mixing.
(2) enzymolysis for the first time: add water and adjust the concentration of serosity so that Horse hoof raw material is 1:2 with the ratio of water, regulates pH value
To 4.0, add compound enzyme I, enzymolysis time 60min, hydrolysis temperature 30 DEG C；Compound enzyme I is 4.5:5.5:2 by enzyme ratio alive:
The cellulase of 3.5, pectase, amylase, saccharifying enzyme composition, amylase enzyme concentration is 8000U/kg (Horse hoof raw material).
(3) enzymolysis for the second time: add water and adjust the concentration of enzymolysis solution for the first time so that obtain after Horse hoof raw material and enzymolysis for the first time
Enzymolysis solution ratio is 1:4, regulates pH, makes pH value at pH 6.0, adds compound enzyme II, and under the conditions of 50 DEG C, enzymolysis 1 is little
Time, obtain second time enzymolysis solution；Described compound enzyme II is made up of amylase, saccharifying enzyme, protease；Wherein amylase, saccharifying
Enzyme, the enzyme ratio alive of protease are 1:1.5:2, and amylase enzyme concentration is 8000U/kg (Horse hoof raw material).
(4) second time enzymolysis solution is boiled 15-20 minute enzyme denaturing, cooling, obtain turbid juice, or to add percentage by weight be 0.5%-1.5%
Adsorbent, filters, makes clear juice.
When preparing clear juice, according to weight * 100% that the gross weight/acquisition juice of crushing juice rate=juice of squeezing out is processed, record enzymolysis
Rear crushing juice rate is up to 85%.
Embodiment 3
A kind of method of complex enzyme hydrolysis Horse hoof fruit juice, step is as follows:
(1) Horse hoof is cleaned, steaming and decocting 30 minutes, after softening, pulverize, add water and size mixing.
(2) enzymolysis for the first time: add water and adjust the concentration of serosity so that Horse hoof raw material is 1:2 with the ratio of water, regulates pH value
To 6.0, add compound enzyme I, enzymolysis time 30min, hydrolysis temperature 40 DEG C；Compound enzyme I is 5.5:4.5:4 by enzyme ratio alive:
The cellulase of 2.5, pectase, amylase, saccharifying enzyme composition, cellulase enzyme concentration is 8000-48000U/kg (Horse hoof
Raw material), pectase enzyme concentration be 8000-48000U/kg (Horse hoof raw material), amylase enzyme concentration be 8000-48000U/kg
(Horse hoof raw material), saccharifying enzyme enzyme concentration are 8000-48000U/kg (Horse hoof raw material).
(3) enzymolysis for the second time: add water and adjust the concentration of enzymolysis solution for the first time so that obtain after Horse hoof raw material and enzymolysis for the first time
Enzymolysis solution ratio is 1:6, regulates pH, makes pH value at pH 4.0, adds compound enzyme II, and under the conditions of 60 DEG C, enzymolysis 1 is little
Time, obtain second time enzymolysis solution；Described compound enzyme II is made up of amylase, saccharifying enzyme, protease；Wherein amylase, saccharifying
Enzyme, the enzyme ratio alive of protease are 2:1:1.5, and amylase enzyme concentration is 8000-48000U/kg (Horse hoof raw material), saccharifying enzyme
Enzyme concentration is 8000-48000U/kg (Horse hoof raw material), and protease enzyme concentration is 5000-40000U/kg (Horse hoof raw material).
(4) second time enzymolysis solution is boiled 15-20 minute enzyme denaturing, cooling, obtain turbid juice, or to add percentage by weight be 0.5%-1.5%
Adsorbent, filters, makes clear juice.
When preparing clear juice, according to weight * 100% that the gross weight/acquisition juice of crushing juice rate=juice of squeezing out is processed, record enzymolysis
Rear crushing juice rate is up to 86%, and crushing juice rate significantly improves.
The Horse hoof fruit juice obtained according to embodiment 1-3 and contrast test embodiment 1-8, carry out subjective appreciation analysis (method is shown in Table 1,
The results are shown in Table 2) and Analysis of Nutritive Composition, result is as follows: from color and luster, structural state, abnormal smells from the patient, flavour and comprehensive acceptance level
It can be seen that in terms of the subjective appreciations such as flavor taste, add complex enzyme zymohydrolysis method relatively single enzyme solution or compound enzyme I enzymolysis
Method or compound enzyme II enzymatic isolation method or the fruit juice that directly mode of squeezing the juice obtains are best；Horse hoof juice nutrient ingredient analysis shows, Horse hoof fruit
Juice nutrient composition content enriches, and interpolation complex enzyme zymohydrolysis method can make a lot of little molecular nutrition material of material dissociate out,
Nutrient composition content is higher than single enzyme solution, compound enzyme I enzymatic isolation method or compound enzyme II enzymatic isolation method.
The method of table 1 subjective appreciation
Table 2 results of sensory evaluation
| Scoring | Color and luster | Abnormal smells from the patient | Flavour | Comprehensive acceptance level |
| Embodiment 1 | 9.3 | 9.1 | 8.6 | 8.8 |
| Embodiment 2 | 8.9 | 8.9 | 7.2 | 9.1 |
| Embodiment 3 | 8.7 | 7.8 | 7.6 | 8.4 |
| Contrast test 1 | 6.5 | 7.3 | 6.8 | 6.4 |
| Contrast test 2 | 8.0 | 7.6 | 7.0 | 7.3 |
| Contrast test 3 | 7.9 | 7.7 | 7.0 | 7.3 |
| Contrast test 4 | 8.1 | 7.8 | 7.1 | 7.5 |
| Contrast test 5 | 8.2 | 7.8 | 7.2 | 7.5 |
| Contrast test 6 | 7.9 | 7.7 | 6.9 | 7.3 |
| Contrast test 7 | 8.5 | 7.9 | 7.5 | 8.1 |
| Contrast test 8 | 8.5 | 7.8 | 7.3 | 8.0 |
Table 3 Horse hoof juice nutrient ingredient table
| Crude protein (g/dL) | Crude fat (g/dL) | Ca(mg/dL) | P(mg/dL) | Fe(mg/dL) | |
| Embodiment 1 | 1.32 | 0.083 | 4.2 | 55.1 | 0.43 |
| Embodiment 2 | 1.13 | 0.090 | 4.3 | 59.2 | 0.45 |
| Embodiment 3 | 0.91 | 0.087 | 3.9 | 56.5 | 0.42 |
| Contrast test 1 | 0.54 | 0.051 | 1.6 | 32.8 | 0.22 |
| Contrast test 2 | 0.82 | 0.064 | 2.2 | 35.7 | 0.27 |
| Contrast test 3 | 0.74 | 0.062 | 2.0 | 38.8 | 0.29 |
| Contrast test 4 | 0.76 | 0.070 | 2.6 | 42.5 | 0.32 |
| Contrast test 5 | 0.81 | 0.071 | 2.8 | 41.9 | 0.33 |
| Contrast test 6 | 0.75 | 0.065 | 2.1 | 43.1 | 0.28 |
| Contrast test 7 | 0.89 | 0.085 | 3.7 | 54.3 | 0.42 |
| Contrast test 8 | 0.86 | 0.084 | 3.6 | 53.8 | 0.39 |
Contrast test 1:
1kg Horse hoof is cleaned, steaming and decocting 20-30min, after softening, pulverize, add water tune according to the part by weight 1:2 of Horse hoof Yu water
Slurry, boils 30min sterilizing, obtains serosity.
When preparing clear juice, according to weight * 100% that the gross weight/acquisition juice of crushing juice rate=juice of squeezing out is processed, record juice
Rate is 50%.
Contrast test 2:
1kg Horse hoof is cleaned, steaming and decocting 20-30min, after softening, pulverize, add water tune according to the part by weight 1:2 of Horse hoof Yu water
Slurry, regulation pH value, to 6.0, adds 10000U (cellulase)/kg (Horse hoof raw material) and carries out enzymolysis, enzymolysis 30min,
Hydrolysis temperature 50 DEG C.Enzymolysis solution boils 15min enzyme denaturing, cooling, obtains turbid juice, or to add percentage by weight be 0.5%-1.5% absorption
Agent is adsorbed, and filters, makes clear juice.
When preparing clear juice, according to weight * 100% that the gross weight/acquisition juice of crushing juice rate=juice of squeezing out is processed, record juice
Rate is 56%.
Contrast test 3:
1kg Horse hoof is cleaned, steaming and decocting 20-30min, after softening, pulverize, add water tune according to the part by weight 1:2 of Horse hoof Yu water
Slurry, regulation pH value, to 6.0, adds 10000U (pectase)/kg (Horse hoof raw material) and carries out enzymolysis, enzymolysis 30min, enzyme
Solve temperature 50 C.Enzymolysis solution boils 15min enzyme denaturing, cooling, obtains turbid juice, or to add percentage by weight be 0.5%-1.5% adsorbent
Absorption, filters, makes clear juice.
When preparing clear juice, according to weight * 100% that the gross weight/acquisition juice of crushing juice rate=juice of squeezing out is processed, record juice
Rate is 55%.
Contrast test 4:
1kg Horse hoof is cleaned, steaming and decocting 20-30min, after softening, pulverize, add water tune according to the part by weight 1:2 of Horse hoof Yu water
Slurry, regulation pH value, to 6.0, adds 10000U (amylase)/kg (Horse hoof raw material) and carries out enzymolysis, enzymolysis 30min, enzyme
Solve temperature 50 C.Enzymolysis solution boils 15min enzyme denaturing, cooling, obtains turbid juice, or to add percentage by weight be 0.5%-1.5% adsorbent
Absorption, filters, makes clear juice.
When preparing clear juice, according to weight * 100% that the gross weight/acquisition juice of crushing juice rate=juice of squeezing out is processed, record juice
Rate is 57%.
Contrast test 5:
1kg Horse hoof is cleaned, steaming and decocting 20-30min, after softening, pulverize, add water tune according to the part by weight 1:2 of Horse hoof Yu water
Slurry, regulation pH value, to 6.0, adds 10000U (saccharifying enzyme)/kg (Horse hoof raw material) and carries out enzymolysis, enzymolysis time 1h,
Hydrolysis temperature 50 DEG C.Enzymolysis solution boils 15min enzyme denaturing, cooling, obtains turbid juice, or to add percentage by weight be 0.5%-1.5% absorption
Agent is adsorbed, and filters, makes clear juice.
When preparing clear juice, according to weight * 100% that the gross weight/acquisition juice of crushing juice rate=juice of squeezing out is processed, record juice
Rate is 58%.
Contrast test 6:
1kg Horse hoof is cleaned, steaming and decocting 20-30min, after softening, pulverize, add water tune according to the part by weight 1:2 of Horse hoof Yu water
Slurry, regulation pH value, to 6.0, adds 10000U (protease)/kg (Horse hoof raw material) and carries out enzymolysis, enzymolysis 30min, enzyme
Solve temperature 50 C.Enzymolysis solution boils 15min enzyme denaturing, cooling, obtains turbid juice, or to add percentage by weight be 0.5%-1.5% adsorbent
Absorption, filters, makes clear juice.
When preparing clear juice, according to weight * 100% that the gross weight/acquisition juice of crushing juice rate=juice of squeezing out is processed, record juice
Rate is 55%.
Contrast test 7:
1kg Horse hoof is cleaned, steaming and decocting 20-30min, after softening, pulverize, add water tune according to the part by weight 1:2 of Horse hoof Yu water
Slurry, regulation pH value, to 6.0, adds compound enzyme I, enzymolysis 30min, hydrolysis temperature 40 DEG C；Compound enzyme I is 1 by enzyme ratio alive:
The cellulase of 1:1:1, pectase, amylase, saccharifying enzyme form.Its cellulase enzyme concentration is 8000-48000U/kg
(Horse hoof raw material).
Prepared enzymolysis solution boils 15min enzyme denaturing, cooling, obtains turbid juice, or to add percentage by weight be 0.5%-1.5% adsorbent,
Filter, make clear juice.When preparing clear juice, according to weight * 100% that the gross weight/acquisition juice of crushing juice rate=juice of squeezing out is processed,
Recording crushing juice rate is 78%.
Contrast test 8:
1kg Horse hoof is cleaned, steaming and decocting 20-30min, after softening, pulverize, add water tune according to the part by weight 1:2 of Horse hoof Yu water
Slurry, regulation pH value, to 6.0, adds compound enzyme II, under the conditions of 60 DEG C, enzymolysis 60min, obtains enzymolysis solution；Described compound
Enzyme II is made up of amylase, saccharifying enzyme, protease；Wherein amylase, saccharifying enzyme, the enzyme ratio alive of protease are 2:1:1.5,
Amylase enzyme concentration is 24000U/kg (Horse hoof raw material).
Prepared enzymolysis solution boils 15min enzyme denaturing, cooling, obtains turbid juice, or to add percentage by weight be 0.5%-1.5% adsorbent,
Filter, make clear juice.When preparing clear juice, according to weight * 100% that the gross weight/acquisition juice of crushing juice rate=juice of squeezing out is processed,
Recording crushing juice rate is 75%.
Above by embodiment, present disclosure is described in detail, but described content has been only presently preferred embodiments of the present invention,
It is not to be regarded as the practical range for limiting the present invention.Any person skilled in the art, without departing from the spirit of the present invention and model
In enclosing, all can do various changes and modification, therefore protection scope of the present invention should be with being as the criterion that claims are defined.
Claims (9)
1. the method that a complex enzyme hydrolysis prepares Horse hoof fruit juice, it is characterised in that described method includes the following:
(A) Horse hoof is cleaned, steaming and decocting, pulverize, size mixing, boiling sterilization, obtain serosity；
(B) enzymolysis: add water and adjust the concentration of serosity, regulation pH value, to 4.0-6.0, adds cellulase, pectase, starch
Enzyme, saccharifying enzyme, protease, hydrolysis temperature 30-60 DEG C, enzymolysis time is 0.5-4h；
(C) enzyme denaturing is boiled after enzymolysis, cooling, obtain turbid juice, or to add percentage by weight be 0.5%-1.5% adsorbent, filter,
Make clear juice.
Method the most according to claim 1, it is characterised in that in described enzymolysis process, cellulase, pectase, amylase,
Saccharifying enzyme, the enzyme of protease live ratio for (4～6): (4～6): (2～6): (2～4): 1.
Method the most according to claim 1, it is characterised in that the enzymolysis of described step (B) is carried out at twice；Wherein
Enzymolysis for the first time: add water and adjust the concentration of serosity so that Horse hoof raw material is 1:2-1:6 with the ratio of water, regulation pH value is extremely
4.0-6.0, add compound enzyme I, enzymolysis time 30-60min, hydrolysis temperature 30-40 DEG C；Compound enzyme I is lived ratio for (4.5～5.5) by enzyme:
(4.5～5.5): (2～4): the cellulase of (2.5～3.5), pectase, amylase, saccharifying enzyme form, cellulase dosage
For 8000-48000U/kg, pectase dosage be 8000-48000U/kg, amylase dosage be 8000-48000U/kg, saccharifying
Enzyme dosage is 8000-48000U/kg.
Enzymolysis for the second time: add water and adjust the concentration of enzymolysis solution for the first time so that the enzymolysis obtained after Horse hoof raw material and for the first time enzymolysis
Liquor ratio value is 1:4-6, regulates pH, makes pH value at pH 4.0-6.0, adds compound enzyme II, under the conditions of 50-60 DEG C, enzymolysis
0.5-3 hour, obtain second time enzymolysis solution；Described composite membrane II is made up of amylase, saccharifying enzyme, protease；Wherein amylase,
Saccharifying enzyme, the enzyme of protease are lived than for (1～2): (1～2): (1～2), amylase dosage be 8000-48000U/kg,
Saccharifying enzyme dosage is 8000-48000U/kg, protease dosage is 5000-40000U/kg.
Method the most according to claim 3, it is characterised in that described compound enzyme I by enzyme live than be 5:5:3:2 cellulase,
Pectase, amylase, saccharifying enzyme form.
Method the most according to claim 3, it is characterised in that described compound enzyme II by enzyme live than be 1:1:1 amylase,
Saccharifying enzyme, protease form.
Method the most according to claim 3, it is characterised in that described first time enzymolysis is to carry out under pH 4.0-5.0.
Method the most according to claim 3, it is characterised in that described method specifically includes:
(1) Horse hoof is cleaned, steaming and decocting, pulverize, add water and size mixing；
(2) enzymolysis for the first time: add water and adjust the concentration of serosity so that Horse hoof raw material is 1:2-1:6 with the ratio of water, regulates pH
Value, makes pH refer at 4.0-6.0, adds compound enzyme I, cellulase preparation: pectase preparation: diastase: saccharifying enzyme system
Agent enzyme lives ratio for 5:5:3:2, enzymolysis time 30-60min, hydrolysis temperature 30-40 DEG C, enzymolysis pH 4.0-5.0, cellulase dosage
It is 8000-48000U/kg, starch for 8000-48000U/kg (for Horse hoof raw material, lower same), pectase dosage
Enzyme dosage is 8000-48000U/kg, saccharifying enzyme dosage is 8000-48000U/kg；
(3) enzymolysis for the second time: add water and adjust the concentration of enzymolysis solution for the first time so that Horse hoof raw material and enzymolysis solution ratio are 1:4-1:6,
Regulation pH, makes pH value at pH 4.0-6.0, adds compound enzyme II, and amylase dosage is 8000-48000U/kg, saccharifying enzyme adds
Amount is 5000-40000U/kg for 8000-48000U/kg, protease dosage, under the conditions of 50-60 DEG C, and enzymolysis 0.5-3 hour,
Obtain second time enzymolysis solution；
(4) secondary enzymolysis liquid is boiled enzyme denaturing, cooling, obtain turbid juice, or to add enzymolysis solution percentage by weight be 0.5%-1.5% absorption
Agent is adsorbed, and filters, makes clear juice.
8. according to the arbitrary described method of claim 1-7, it is characterised in that described adsorbent is gauze or activated carbon or silica gel or divides
Son sieve.
9. according to the arbitrary described method of claim 1-7, it is characterised in that the pH adjusting agent used during described regulation pH is
Food stage citric acid or dilute hydrochloric acid.
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| CN108094778B (en) * | 2018-02-27 | 2021-05-11 | 江南大学 | Preparation method of functional water chestnut peel composite fruit juice beverage and product thereof |
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