CN1480023A

CN1480023A - Technique for preparing and planting edible fungus by using biotechnology to restraint bacterium

Info

Publication number: CN1480023A
Application number: CNA031337724A
Authority: CN
Inventors: 王显亮
Original assignee: Individual
Current assignee: Individual
Priority date: 2003-07-21
Filing date: 2003-07-21
Publication date: 2004-03-10

Abstract

A biological technology for seeding and culturing edible fungus (domestomycetes) features that its medium material is prepared from saw dust, dried thallus powder, gypsum powder and water. Said dried thallus powder is prepared through preparing culture medium from rice bran, gypsum powder, calcium perphosphate, and water, loading in bag or bottle, high-pressure sterilizing, cooling, inoculating ligninolytic enzyme, culturing at 45 deg.c for 10-12 days to obtain thallus, baking and pulverizing. Its advantages are less investment and high successful rate.

Description

Edible fungus living being bacterium restraining seed breeding, culture technique

Technical field

The invention belongs to the production and the application of biotechnology dielectric material, particularly a kind of edible fungus living being bacterium restraining seed breeding, culture technique.

Background technology

At present, in the domestic production of hybrid seeds cultivation edible mushroom, all adopt the asepticize operation, requirement will have higher technical merit, perfect infrastructure and good condition of culture.Wherein requiring has: culturing room, transfer room, sterile board, chemical sterilization medicament, the whole production program is all finished under aseptic condition.As Chinese patent 97108197.2 disclosed a kind of edible mashroom cultivating and cultivation methods; its production of hybrid seeds operation comprises system medium, sterilization, inoculation and training pipe; the cultivation operation comprises the system cultivate material, forces fermentation, sows and the training pipe; the raw material of medium comprises rice and millet or wheat or jowar, glucose, gypsum, ash, dried earthworms muck powder, rice washing water, boll hull, lime and dry vegetalbe garden mould powder, and the raw material of system cultivate material comprises straw or wheat straw or corn stalk or weeds, dry vegetalbe garden mould powder, lime, gypsum, salt, ash, potash fertilizer, wood chip or rice washing water.In case the production of hybrid seeds culture technique of this edible mushroom has operating personnel because careless manipulation or facility imperfection will cause pollution in wide area, and the lighter's success rate is low, and weight person all scraps, make to produce to suffer heavy losses.

Summary of the invention

The objective of the invention is for can under the situation that does not have sterile facilities and chemical bactericide, turning out pure edible fungus species and cultivation bag, make kind of mushroom devoid of risk and reduce kind of a mushroom investment, a kind of edible fungus living being bacterium restraining seed breeding, culture technique are provided.

Edible fungus living being bacterium restraining seed breeding of the present invention, culture technique content Description:

Edible fungus living being bacterium restraining seed breeding of the present invention, culture technique is characterized in that: dielectric material: adopt sawdust 68～79%, thalline dry powder 20～30%, land plaster 1%; Add water during use, dielectric material with the water ratio is: 1: 1.2.

Cultivate edible mushroom suitable bacterium is arranged, the culture medium prescription that suitable bacterium is arranged: rice bran 98%, land plaster 1%, superphosphate 1%, water 85%, pack or bottle, through autoclaving, the cooling back is inserted lignin and is decomposed mould under aseptic condition, under 45 ℃ of conditions of temperature, cultivate and made thalline in 10～12 days, with thalline oven dry, pulverizing, make thalline dry powder again.

Invention edible fungus living being bacterium restraining seed breeding, culture technique, be applicable to the cultivation wood decay fungi, the production of hybrid seeds and cultivation as the wood decay fungi of mushroom, Asparagus, woodear, white fungus, Hericium erinaceus, flat mushroom, Xingbao mushroom, Pleuotus nebrodensis Quel, HUAZIGU, coprinus comatus, glossy ganoderma etc., it is a kind of opening inoculation that does not need chemical agent, do not need infrastructure such as dressing cubicle, cooling chamber, desinfection chamber, have little, the devoid of risk of investment, characteristics that success rate is high, and alleviated operator's labour intensity widely.

Embodiment

Flat mushroom, mushroom Growing Technique

Batching: sawdust 78%, wheat bran 20%, land plaster 2%, dielectric material and water;

With above-mentioned sawdust, wheat bran, land plaster, in pack or the bottle, compacting, the dielectric material 1～2cm that spreads material-water ratio in the above and be 1: 1.2 is thick, flatten autoclaving 2 hours, cooling, inoculation;

Inoculation method: will expect below the temperature drop to 30 ℃, open inoculation, indoor, outdoor all can, be applicable to make two, three-class strain and fruiting bag; Insert bacterial classification in the bag below 30 ℃, encapsulate, cultivate in the material temperature; Condition of culture requires between 5～28 ℃ of no direct light, air humidity below 80%, the temperature.

Claims

1, a kind of edible fungus living being bacterium restraining seed breeding, culture technique is characterized in that: dielectric material: adopt sawdust 68～79%, thalline dry powder 20～30%, land plaster 1%; Add water during use, dielectric material with the water ratio is: 1: 1.2.

2, edible fungus living being bacterium restraining seed breeding as claimed in claim 1, culture technique, it is characterized in that said dielectric material is: cultivating edible mushroom has suitable bacterium, the culture medium prescription that suitable bacterium is arranged: rice bran 98%, land plaster 1%, superphosphate 1%, water 85%, pack or bottle, through autoclaving, the lignin decomposition is inserted in the cooling back under aseptic condition mould, cultivates under 45 ℃ of conditions of temperature and made thalline in 10～12 days, with thalline oven dry, pulverizing, make thalline dry powder again.

3, the production of hybrid seeds of a kind of flat mushroom, mushroom, culture technique is characterized in that: batching: sawdust 78%, wheat bran 20%, land plaster 2%, dielectric material and water;