CN1387855A - 甘草多糖粉针剂 - Google Patents
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Abstract
甘草多糖粉针剂由甘草的根、茎加水煮沸,提取清液,脱色;浓缩,醇沉析;离心沉析物;烘干、研磨、过筛、得粗多糖;再加水,过滤溶胀;再离心后取上清液;加入Sevage试剂除去蛋白,用无离子水透析;DEAE－Cellulose(DE－32)柱层析;洗脱浓缩,醇沉析,离心,醇洗,真空干燥得到甘草多糖粉针剂。本品能激活巨噬细胞,TB淋巴细胞,增强体液免疫及全身免疫功能和抑制肿瘤和爱滋病。
Description
本发明属于来源于植物材料中药的提取物，特别涉及从甘草，有效部位提纯精制而成的多糖物质。是一种涉及有关治疗肿瘤，增强人体免疫功能，对抑制爱滋病有较好疗效的甘草多糖粉针剂。
目前，肿瘤是常见的多发病，本病在国内外西药治疗中尚无特效药，而且药物价格昂贵，使多数患者生畏。近年来，我国中医治疗肿瘤研究十分活跃，从基础理论到临床治疗都取得了一定的进展。从全国各地报道来看，疗效不一，仁智各见。为此如何提高临床疗效，探取治疗规律，乃是当务之急。对爱滋病的治疗，国内外亦无发现特别有效的药物。
本发明的目的是，提供一种调节人体免疫功能抑制肿瘤及爱滋病的甘草多糖粉针剂。经大量科学实验证明，本品不仅能激活巨噬细胞，TB淋巴细胞，从而增强体液免疫和细胞免疫的双重功能，同时，甘草多糖对从肿瘤细胞端粒作用及对爱滋病的逆转录酶具有抑制作用，使基因组(DNA或RNA)，无法正常复制，从而起到对病毒或肿瘤的抑制或直接灭活，是改进全身免疫功能和更有效地抑制肿瘤和爱滋病的目的。
本发明是一种不同于合成药物的，以增强人体免疫功能抑制肿瘤及爱滋病为目的的天然绿色药物。
本发明是这样实现的：甘草多糖粉针剂的制备过程如下：一、原料处理取甘草根、茎切割，用水冲洗去杂。二、甘草多糖粉针剂制备1.原料加水，100℃煮沸7～8小时，滤除杂质，获取清液。2.经活性炭脱色。3.脱色液浓缩，用醇沉析。4.离心获取沉析物。5.沉析物烘干、研磨、过筛、获取粗多糖。6.粗多糖加水，4℃过滤溶胀。7.离心，取上清液。8.加Sevage试剂除蛋白。9.用无离子水透析。10.经DEAE-Cellulose(DE-32)柱层析。11.洗脱液浓缩。12.用醇沉析多糖。13.离心，获取沉析物。14.用醇洗涤2次。15.真空干燥。16.研磨成白色粉末状多糖。17.冷冻干燥法制备粉针剂。三、包装1.将白色粉末状多糖粉针剂装入安培瓶中，每瓶1mg，经物理方法灭菌。2.包装入盒，封戳。
本发明的特点是：甘草多糖的理化及生物学特性1.理化特性：
(1)不含氮，无结合蛋白，主要组分为葡萄糖的葡聚糖。
(2)[α]D28：+120°(c0.1，H2O)的比旋度。
(3)红外光谱分析，吸收峰(cm-1)855～833为α-D葡吡喃
糖，甘草多糖具850.6230吸收峰，表明其属α-D-吡喃
葡聚糖。
(4)经核磁共振测定，表明为2-(1.4)键连接的葡聚糖。
(5)经甲基化与质谱分析，其分子量为37000 Dalton
分子结构为
(6)无色、无味粉末状物质。
(7)易溶于水。2.生物学特性
(1)对实体痛S-180抑瘤率可达85％。
(2)巨噬细胞吞噬率可提高66％。
(3)对腺病毒III型感染细胞保护率提高74.5％，对柯窿奇病毒
感染细胞保护率提高59.1％。
(4)对于爱滋病毒抑制率达36.2％。
经大量科学实验证明，本品不仅能激活巨噬细胞，TB淋巴细胞，从而增强体液免疫和细胞免疫的双重功能，同时，甘草多糖对从肿瘤细胞端粒作用及对爱滋病的逆转录酶具有抑制作用，使基因组(DNA或RNA)，无法正常复制，从而起到对病毒或肿瘤的抑制或直接灭活，是改进全身免疫功能和更有效地抑制肿瘤和爱滋病的目的。
图1是甘草多糖粗多糖制备工艺流程图：图2是甘草多糖粉针剂制备工艺流程图
本产品经毒理学安全性评价检测报告确认无毒物质。
本产品经保健品的功效成分、稳定性测试确认品质稳定。
本产品经对抗病毒研究有抑制作用和直接灭活作用，对巨噬细胞吞噬功能研究有明显促进作用有免疫促进作用，对抑瘤作用试验可见大片变性坏死细胞及造性分布的核固缩。
本产品经对抗AIDS药物检测报告显示：对病毒复制有一定抑制作用，对病毒抑制率为36.2％。
Claims (2)
1、一种甘草多糖粉针剂，其特征在于：由以下制备方法过程得到的，取甘草的根、茎，切割成小块，用水冲洗去杂质；加入水，加热100℃煮沸7-8小时，滤除杂质，提取清液；再经活性炭脱色；脱色后的液体进行浓缩，再用醇沉析；采用离心方法获取沉析物；再将沉析物烘干、研磨、过筛、获得粗多糖；再由粗多糖加水，在温度4℃下过滤，溶胀；再经离心后，取上清液；再加入Sevage试剂除去蛋白；再用无离子水透析；再经DEAE-Cellulose(DE-32)柱层析；再经洗脱液浓缩，用醇沉析多糖，再经离心获得沉析物；再用醇洗涤2次；再经真空干燥，研磨，得到白色粉末状的物质再采用干燥法制备粉针剂即为甘草多糖粉针剂。
2、根据权利要求1所述的甘草多糖粉针剂，其制备方法为：取甘草的根、茎，切割成小块，用水冲洗去杂质；加入水，加热100℃煮沸7～8小时，滤除杂质，提取清液；再经活性炭脱色；脱色后的液体进行浓缩，再用醇沉析；采用离心方法获取沉析物；再将沉析物烘干、研磨、过筛、获得粗多糖；再由粗多糖加水，在温度4℃下过滤，溶胀；再经离心后，取上清液；再加入Sevage试剂除去蛋白；再用无离子水透析；再经DEAE-Cellulose(DE-32)柱层析；再经洗脱液浓缩，用醇沉析多糖，再经离心获得沉析物；再用醇洗涤2次；再经真空干燥，研磨，得到白色粉末状甘草多糖，再采用干燥法制备粉针剂。
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|---|---|---|---|---|
| CN108191988A (zh) * | 2017-12-26 | 2018-06-22 | 浦江县美泽生物科技有限公司 | 甘草多糖提取工艺 |
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| CN108191988A (zh) * | 2017-12-26 | 2018-06-22 | 浦江县美泽生物科技有限公司 | 甘草多糖提取工艺 |
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