CN1312272C - Biodegradation method for preventing and eliminating Eupatorium adenophorum spreng - Google Patents
Biodegradation method for preventing and eliminating Eupatorium adenophorum spreng Download PDFInfo
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- CN1312272C CN1312272C CNB2005100210833A CN200510021083A CN1312272C CN 1312272 C CN1312272 C CN 1312272C CN B2005100210833 A CNB2005100210833 A CN B2005100210833A CN 200510021083 A CN200510021083 A CN 200510021083A CN 1312272 C CN1312272 C CN 1312272C
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Abstract
The present invention relates to a biodegradation method for preventing and eliminating eupatorium adenophorum. Cap fungus kingcup 2-1 is used as a degradation strain; crushed eupatorium adenophorum or a mixture of the crushed eupatorium adenophorum and rice straw or/and wheat bran or/and corn cobs are added for culture; the biodegradation of eupatorium adenophorum is carried out in the process of the mycelial growth and the formation of a fruiting body; then, a cap fungus fruiting body is obtained, and residues are completely converted into soil organic matter. The biodegradation method for preventing and eliminating eupatorium adenophorum has two specific operation modes: a paving inoculation mode and a bacterial bag inoculation mode, wherein in the paving inoculation mode, the eupatorium adenophorum particles or a mixture of the eupatorium adenophorum particles and other auxiliary materials is paved on farmland to carry out inoculation, mycelial culture, earth covering and the collection of the fruiting body; in the bacterial bag inoculation mode, the eupatorium adenophorum particles or a mixture of the eupatorium adenophorum particles and other auxiliary materials is filled into a bacterial bag for sterilization and then carry out inoculation, mycelial culture, fruiting at both ends, the collection of a fruiting body 1-2 tide, bag shedding for earth covering, culture and the collection of the fruiting body in an inoculating chamber according to the requirements of bacteria free operation.
Description
Technical field
The invention belongs to biological process degraded environmental pollutant field, particularly a kind of noxious plant---biological degradation method of Hemp Eupatorium of, contaminate environment toxic to containing.
Background technology
Hemp Eupatorium (or cercis Herba Lycopi) belongs to composite family Eupatorium per nnial herb, extremely strong vitality and natural adaptation ability are arranged, reproductivity is strong, velocity of propagation is fast, annual surprising speed with 30 kms outwards spreads from its edge, range of distribution in China southwest, make meadow, forest land, arable land serious degradation, the husbandry underproduction reduction of income, forfeiture of environmental ecology service function or decline, financial loss is serious, is put into one of four big malignant weeds in " control of external harmful organism and international biological and ecological methods to prevent plant disease, pests, and erosion pact ".At present, Hemp Eupatorium has been spreaded all over Yunnan Province of China, Guizhou, Sichuan, Tibet, Guangxi etc. and has economized (district), and only Yunnan just has within the border the Hemp Eupatorium growth on more than 10 ground (state), the about 2,470 ten thousand hectares of soils, more than 90 counties and cities, and potential danger is very big.This plant has toxicity, gives out special smell, can bring out livestocks such as ox, horse and suffer from asthmas and cause death, and cattle and sheep have eaten and can cause mashed lung and cause death; With its branches and leaves packing ring, can cause that the oxen and horses rot hoof.
Because Hemp Eupatorium hazardness is very big, control to Hemp Eupatorium causes extensive concern, since the seventies in 20th century, has carried out the test of preventing and kill off Hemp Eupatorium in a large number, comprise artificial and machinery is prevented and kill off, chemical control, natural enemy control, vegetation ecological are alternative etc., but actual effect is undesirable; Agriculturally also have and attempt utilizing Hemp Eupatorium to produce fertilizer or feed; In industrial aspect, utilize Hemp Eupatorium to produce reports such as Xylitol.But up to the present, these methods all fail to promote, and the harm of Hemp Eupatorium is not effectively controlled.
Summary of the invention
The present invention is directed to the deficiency that prior art exists, a kind of biological degradation method of preventing and kill off Hemp Eupatorium is provided, this kind method can not only be prevented and kill off the cercis Herba Lycopi, eliminate the toxicity of Hemp Eupatorium, and can produce a large amount of edible mushroomss, produce good economic benefit, be easy to promote, make the residue of degraded change the organic matter that strengthens soil fertility into simultaneously.
Technical scheme of the present invention: 2-1 is a degradation bacteria strains with flat mushroom kind gold phoenix, with the Hemp Eupatorium after pulverizing or the Hemp Eupatorium after pulverizing and straw or/and wheat bran or/and the mixture of corn cob as culture material, insert golden phoenix 2-1 flat mushroom strain and cultivate, in the process of mycelial growth and formation sporophore, carried out the biological degradation of Hemp Eupatorium.Gold phoenix 2-1 flat mushroom strain is that Inst. of Soil Fertilizer, Sichuan Academy of Agriculture Science is the parent with needle mushroom and Pleurotus sajor-caju, merge new variety between the first in the world edible Cordycepps that the application cell integration technology selects, obtained the kind certification of fitness that Sichuan Province variety of crops validation board in 1998 issues, this flat mushroom strain is wide warm type annually cultivating high-yield variety, use at large-scale promotion of whole nation rapidly with its good characteristic, can buy (for example, can buy) by commodity to Sichuan Academy of Agricultural Sciences.
The biological degradation method of preventing and kill off Hemp Eupatorium of the present invention, concrete operations have dual mode, and a kind of is floor file inoculation formula, and another kind of is that the bacterium bag is inoculated formula.
One, floor file inoculation formula
Floor file inoculation formula may further comprise the steps:
1, collection of Hemp Eupatorium and pulverizing;
2, the Hemp Eupatorium after adjust pulverizing or pulverize after Hemp Eupatorium and straw or/and wheat bran or/and the water content of the mixture of Mi Xin, making its aqueous weight percentage is 62～75%;
3, will meet the Hemp Eupatorium particle of water content or Hemp Eupatorium and straw or/and wheat bran or/and the mixture of corn cob be laid on the arable land;
4, insert golden phoenix 2-1 flat mushroom strain, in 10 ℃～30 ℃ following shadings cultivation of preserving moisture, when golden phoenix 2-1 mycelia grow at least institute's lay Hemp Eupatorium thickness 2/3rds the time, cover the fine soil grain of 2～4 centimetres of thickness, continue shading and preserve moisture, under 0 ℃～35 ℃, be cultured to and grow sporophore;
5, pluck golden phoenix 2-1 sporophore.
According to test; adopt the floor file vaccination ways to the Hemp Eupatorium biodegrade; institute's lay Hemp Eupatorium particle or Hemp Eupatorium and straw or/and wheat bran or/and the thickness of the mixture of corn cob is 25～35 centimetres of the bests, Hemp Eupatorium that the golden phoenix 2-1 flat mushroom strain that is inserted is institute's lay or Hemp Eupatorium and straw or/and wheat bran or/and 1/5～1/4 the best of the mixture weight of corn cob.
Two, bacterium bag inoculation formula
Bacterium bag inoculation formula may further comprise the steps:
1, collection of Hemp Eupatorium and pulverizing;
2, the Hemp Eupatorium after adjust pulverizing or pulverize after Hemp Eupatorium and straw or/and wheat bran or/and the water content of the mixture of corn cob, making its aqueous weight percentage is 62～75%;
3, will meet the Hemp Eupatorium particle of water content or Hemp Eupatorium and straw or/and wheat bran or/and the mixture of corn cob is packed into the bacterium bag, put into the sterilising plant sterilization;
4, the bacterium bag after will sterilizing requires to insert golden phoenix 2-1 flat mushroom strain at transfer room according to aseptic technique;
5, postvaccinal bacterium bag is moved into culturing room, at room temperature is cultured to the mycelia purseful, treat that mycelia is covered with after, change the bacterium bag over to shade and manage, allow its two fruiting;
6, wait to gather behind sporophore 1～2 tide, remove the outer bag of culture bag, imbed in the soil, 2～4 centimetres of fine soil grains of cladding thickness continue shading and preserve moisture, and cultivate down at 0 °～35 ℃, until growing sporophore;
7, pluck golden phoenix 2-1 sporophore.
Adopt bacterium bag vaccination ways to the Hemp Eupatorium biodegrade, the Hemp Eupatorium of the bacterium bag of packing into both can be sterilized under normal pressure, also can under high pressure sterilize, but generally sterilize under normal pressure.If sterilize under normal pressure, temperature is controlled at 100 ℃, and the time is 6～8 hours, and sterilization postcooling to room temperature is transported to the transfer room inoculation.
The present invention has following beneficial effect:
1,, prevents and kill off the cercis Herba Lycopi by biological degradation.
2, Hemp Eupatorium no longer has toxicity and original special odor after golden phoenix 2-1 flat mushroom degraded, can not produce secondary pollutant to environment, and the product after its metabolism is converted into the soil organism fully, and the structure of can improving the soil strengthens soil fertility.
3, the golden phoenix 2-1 flat mushroom that is grown is through the edible nontoxicity of animal and human, delicious flavour.
4, to the Hemp Eupatorium biodegrade, 1 kilogram of Hemp Eupatorium siccative can be produced 1～4 kilogram of golden phoenix 2-1 mushroom carpophore, has good economic benefits.
5, degradation method is simple, is easy to grasp, and required equipment mainly is the wood chip pulverizer, thereby invests for a short time, promotes easily.
6, as long as this method obtains promoting, Hemp Eupatorium will be excavated utilization in a large number, and its harm to environmental ecology will be effectively controlled.
Embodiment
Embodiment 1
The biological degradation of preventing and kill off Hemp Eupatorium may further comprise the steps:
(1) excavates or purchases the cercis Herba Lycopi;
(2) be ground into particle with the wood chip pulverizer after the Hemp Eupatorium drying that will excavate or purchase;
(3) water content of the Hemp Eupatorium after the above-mentioned pulverizing of adjustment, making its aqueous weight percentage is 75%;
(4) the Hemp Eupatorium particle that will meet water content is laid on the arable land, and the thickness of lay is 25 centimetres;
(5) insert golden phoenix 2-1 flat mushroom strain, 1/5 of the Hemp Eupatorium weight that the golden phoenix 2-1 flat mushroom strain that is inserted is institute's lay, inoculation back is in 10 ℃ of following shadings cultivation of preserving moisture, when golden phoenix 2-1 mycelial growth to the Hemp Eupatorium thickness of institute's lay 2/3rds the time, cover the fine soil grain of 2 centimetres of thickness, continue shading and preserve moisture, under 0 ℃, be cultured to and grow sporophore;
(6) pluck the sporophore of to gather 3～4 times after the sporophore maturation.Can produce 2 kilograms of fresh flat mushroom sporophores with 1 kilogram of siccative calculating.After sporophore was gathered and finished, the Hemp Eupatorium residue after the degraded was converted into the organic matter of soil naturally, has improved soil.
Embodiment 2
The biological degradation of preventing and kill off Hemp Eupatorium may further comprise the steps:
(1) excavates or purchases the cercis Herba Lycopi;
(2) Hemp Eupatorium that will excavate or purchase directly is ground into particle with the wood chip pulverizer;
(3) water content of the Hemp Eupatorium after the above-mentioned pulverizing of adjustment, making its aqueous weight percentage is 65%;
(4) the Hemp Eupatorium particle that will meet water content is laid on the arable land, and the thickness of lay is 35 centimetres;
(5) insert golden phoenix 2-1 flat mushroom strain, 1/4 of the Hemp Eupatorium weight that the golden phoenix 2-1 flat mushroom strain that is inserted is institute's lay, inoculation back is in 20 ℃ of following shadings cultivation of preserving moisture, when golden phoenix 2-1 mycelial growth to the Hemp Eupatorium thickness of institute's lay 4/5ths the time, cover the fine soil grain of 4 centimetres of thickness, continue shading and preserve moisture, under 20 ℃, be cultured to and grow sporophore;
(6) pluck the sporophore of to gather 3～4 times after the sporophore maturation.Can produce 1.8 kilograms of fresh flat mushroom sporophores with 1 kilogram of siccative calculating.After sporophore was gathered and finished, the Hemp Eupatorium residue after the degraded was converted into the organic matter of soil naturally, has improved soil.
Embodiment 3
The biological degradation of preventing and kill off Hemp Eupatorium may further comprise the steps:
(1) excavates or purchases the cercis Herba Lycopi;
(2) Hemp Eupatorium that will excavate or purchase directly is ground into particle with the wood chip pulverizer;
(3) compound of preparation Hemp Eupatorium and dry rice straw joint, the weight ratio of Hemp Eupatorium and dry rice straw joint is 3: 1, adjusts the water content of above-mentioned compound, making its aqueous weight percentage is 70%;
(4) the above-mentioned compound that meets water content is laid on the arable land, the thickness of lay is 30 centimetres;
(5) insert golden phoenix 2-1 flat mushroom strain, 1/4 of the compound weight that the golden phoenix 2-1 flat mushroom strain that is inserted is institute's lay, inoculation back is in 30 ℃ of following shadings cultivation of preserving moisture, when golden phoenix 2-1 mycelial growth when identical with the thickness of the compound of lay, cover the fine soil grain of 3 centimetres of thickness, continue shading and preserve moisture, under 30 ℃, be cultured to and grow sporophore;
(6) pluck the sporophore of to gather 3～4 times after the sporophore maturation.Can produce 1 kilogram of fresh flat mushroom sporophore with 1 kilogram of siccative calculating.After sporophore was gathered and finished, the Hemp Eupatorium residue after the degraded was converted into the organic matter of soil naturally, has improved soil.
Embodiment 4
The biological degradation of preventing and kill off Hemp Eupatorium may further comprise the steps:
(1) excavates or purchases the cercis Herba Lycopi;
(2) be ground into particle with the wood chip pulverizer after the Hemp Eupatorium drying that will excavate or purchase;
(3) water content of the Hemp Eupatorium after adjustment is pulverized, making its aqueous weight percentage is 75%;
(4) the Hemp Eupatorium particle that will the meet water content bacterium bag of packing into is put into the sterilising plant sterilization, and sterilization is carried out under normal pressure, and temperature is controlled at 100 ℃, and the time is 8 hours, and the sterilization postcooling is transported to transfer room then to room temperature;
(5) the bacterium bag after will sterilizing requires to insert golden phoenix 2-1 flat mushroom strain at transfer room according to aseptic technique;
(6) postvaccinal bacterium bag is moved into culturing room, under room temperature (natural temperature), be cultured to the mycelia purseful, after treating that mycelia is covered with, changing the bacterium bag over to shade manages, allow its two fruiting, at this moment the sporophore of gathering 1～2 tide, its output calculate with 1 kilogram of siccative can produce 1.8 kilograms of fresh flat mushroom sporophores;
(7) wait to gather behind sporophore 1～2 tide, remove the outer bag of culture bag, imbed in the soil, 2 centimetres of fine soil grains of cladding thickness continue shading and preserve moisture, and cultivate down at 5 ℃, until growing sporophore;
(8) pluck the sporophore of to gather 3～4 times after the sporophore maturation.At this moment calculate with 1 kilogram of siccative and also can produce 2 kilograms of fresh flat mushroom sporophores.After sporophore was gathered and finished, the Hemp Eupatorium residue after the degraded was converted into the organic matter of soil naturally, has improved soil.
Embodiment 5
The biological degradation of preventing and kill off Hemp Eupatorium may further comprise the steps:
(1) excavates or purchases the cercis Herba Lycopi;
(2) Hemp Eupatorium that will excavate or purchase directly is ground into particle with the wood chip pulverizer;
(3) compound of preparation Hemp Eupatorium and wheat bran, the weight ratio of Hemp Eupatorium and wheat bran is 9: 1, adjusts the water content of compound, making its aqueous weight percentage is 62%;
(4) mixture particle that will the meet water content bacterium bag of packing into is put into the sterilising plant sterilization, and sterilization is carried out under normal pressure, and temperature is controlled at 100 ℃, and the time is 7 hours, and the sterilization postcooling is to room temperature;
(5) the bacterium bag after will sterilizing requires to insert golden phoenix 2-1 flat mushroom strain at transfer room according to aseptic technique;
(6) postvaccinal bacterium bag is moved into culturing room, under room temperature (natural temperature), be cultured to the mycelia purseful, after treating that mycelia is covered with, changing the bacterium bag over to shade manages, allow its two fruiting, at this moment the sporophore of gathering 1～2 tide, its output calculate with 1 kilogram of siccative can produce 2 kilograms of fresh flat mushroom sporophores;
(7) wait to gather behind sporophore 1～2 tide, remove the outer bag of culture bag, imbed in the soil, 4 centimetres of fine soil grains of cladding thickness continue shading and preserve moisture, and cultivate down at 25 ℃, until growing sporophore;
(8) pluck the sporophore of to gather 3～4 times after the sporophore maturation.At this moment calculate with 1 kilogram of siccative and also can produce 2 kilograms of fresh flat mushroom sporophores.After sporophore was gathered and finished, the Hemp Eupatorium residue after the degraded was converted into the organic matter of soil naturally, has improved soil.
Embodiment 6
The biological degradation of preventing and kill off Hemp Eupatorium may further comprise the steps:
(1) excavates or purchases the cercis Herba Lycopi;
(2) Hemp Eupatorium that will excavate or purchase directly is ground into particle with the wood chip pulverizer;
(3) compound of preparation Hemp Eupatorium and corn cob, straw powder, in the compound, the weight percentage of Hemp Eupatorium is 80%, the weight percentage of corn cob is 10%, the weight percentage of straw powder is 10%, adjusts the water content of compound, and making its aqueous weight percentage is 65%;
(4) mixture particle that will the meet water content bacterium bag of packing into is put into the sterilising plant sterilization, and sterilization is carried out under normal pressure, and temperature is controlled at 100 ℃, and the time is 6 hours, and the sterilization postcooling is to room temperature;
(5) the bacterium bag after will sterilizing requires to insert golden phoenix 2-1 flat mushroom strain at transfer room according to aseptic technique;
(6) postvaccinal bacterium bag is moved into culturing room, under room temperature (natural temperature), be cultured to the mycelia purseful, after treating that mycelia is covered with, changing the bacterium bag over to shade manages, allow its two fruiting, at this moment the sporophore of gathering 1～2 tide, its output calculate with 1 kilogram of siccative can produce 1.5 kilograms of fresh flat mushroom sporophores;
(7) wait to gather behind sporophore 1～2 tide, remove the outer bag of culture bag, imbed in the soil, 3 centimetres of fine soil grains of cladding thickness continue shading and preserve moisture, and cultivate down at 35 ℃, until growing sporophore;
(8) pluck the sporophore of to gather 2～3 times after the sporophore maturation.At this moment calculate with 1 kilogram of siccative and also can produce 1.6 kilograms of fresh flat mushroom sporophores.After sporophore was gathered and finished, the Hemp Eupatorium residue after the degraded was converted into the organic matter of soil naturally, has improved soil.
Claims (4)
1, a kind of biological degradation method of preventing and kill off Hemp Eupatorium, it is characterized in that with flat mushroom kind gold phoenix 2-1 be degradation bacteria strains, with the Hemp Eupatorium after pulverizing as culture material, adopt the floor file vaccination ways to insert golden phoenix 2-1 bacterial classification and cultivate, in the process of mycelial growth and formation sporophore, carried out the biological degradation of Hemp Eupatorium.
2, the biological degradation method of Hemp Eupatorium according to claim 1 is characterized in that may further comprise the steps:
(1) collection of Hemp Eupatorium and pulverizing;
(2) water content of the Hemp Eupatorium after adjustment is pulverized, making its aqueous weight percentage is 62～75%;
(3) the Hemp Eupatorium particle that will meet water content is laid on the arable land;
(4) insert golden phoenix 2-1 flat mushroom strain, in 10 ℃～30 ℃ following shadings cultivation of preserving moisture, when golden phoenix 2-1 mycelia grow at least institute's lay Hemp Eupatorium thickness 2/3rds the time, cover the fine soil grain of 2～4 centimetres of thickness, continue shading and preserve moisture, under 0 ℃～35 ℃, be cultured to and grow sporophore;
(5) pluck golden phoenix 2-1 sporophore.
3, the biological degradation method of Hemp Eupatorium according to claim 2 is characterized in that the thickness of Hemp Eupatorium particle lay on ploughing is 25～35 centimetres.
4, according to the biological degradation method of claim 2 or 3 described Hemp Eupatoriums, 1/5～1/4 of the Hemp Eupatorium weight that the golden phoenix 2-1 flat mushroom strain that it is characterized in that being inserted is institute's lay.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100210833A CN1312272C (en) | 2005-06-15 | 2005-06-15 | Biodegradation method for preventing and eliminating Eupatorium adenophorum spreng |
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2005100210833A CN1312272C (en) | 2005-06-15 | 2005-06-15 | Biodegradation method for preventing and eliminating Eupatorium adenophorum spreng |
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| CN1312272C true CN1312272C (en) | 2007-04-25 |
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| CNB2005100210833A Expired - Fee Related CN1312272C (en) | 2005-06-15 | 2005-06-15 | Biodegradation method for preventing and eliminating Eupatorium adenophorum spreng |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106011044A (en) * | 2016-06-27 | 2016-10-12 | 合肥福泉现代农业科技有限公司 | Method for preparing pleurotus citrinopileatus liquid strain through pleurotus citrinopileatus culture medium based on eupatorium adenophorum |
| CN114711087B (en) * | 2022-03-17 | 2023-06-06 | 新疆理工学院 | Method for cultivating edible fungi by using crop straw and application |
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2005
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Patent Citations (4)
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|---|---|---|---|---|
| CN1037440A (en) * | 1988-05-05 | 1989-11-29 | 刘学系 | Method with tobacco or Eupatorium adenophorum cultivating flammulina velutipes or Hericium erinaceus |
| CN1068699A (en) * | 1991-07-20 | 1993-02-10 | 昆明食用菌研究开发中心 | The method of cultivating mushroom with waste edible fungus cultivation compost |
| CN1486613A (en) * | 2003-08-14 | 2004-04-07 | 南京农业大学 | Batch production process of biological herbicide |
| CN1582655A (en) * | 2004-06-15 | 2005-02-23 | 中国科学院植物研究所 | Method for preventing invasion and spread of herba licopi |
Non-Patent Citations (5)
| Title |
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| 利用紫茎兰可栽培食用菌 班秀文，贵州科技报，第003版期 2003 * |
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