CN1310767A - 基于重组杆状病毒的杀虫剂 - Google Patents
基于重组杆状病毒的杀虫剂 Download PDFInfo
- Publication number
- CN1310767A CN1310767A CN99807162A CN99807162A CN1310767A CN 1310767 A CN1310767 A CN 1310767A CN 99807162 A CN99807162 A CN 99807162A CN 99807162 A CN99807162 A CN 99807162A CN 1310767 A CN1310767 A CN 1310767A
- Authority
- CN
- China
- Prior art keywords
- insect
- sequence
- baculovirus
- coding
- promotor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000701447 unidentified baculovirus Species 0.000 title claims abstract description 73
- 239000002917 insecticide Substances 0.000 title abstract description 6
- 241000238631 Hexapoda Species 0.000 claims abstract description 118
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 71
- 231100000765 toxin Toxicity 0.000 claims abstract description 68
- 239000003053 toxin Substances 0.000 claims abstract description 67
- 238000000034 method Methods 0.000 claims abstract description 42
- 241000500437 Plutella xylostella Species 0.000 claims abstract description 33
- 239000000203 mixture Substances 0.000 claims abstract description 31
- 230000002147 killing effect Effects 0.000 claims abstract description 22
- 230000000749 insecticidal effect Effects 0.000 claims abstract description 19
- 108010076504 Protein Sorting Signals Proteins 0.000 claims description 79
- 108700012359 toxins Proteins 0.000 claims description 67
- 230000008521 reorganization Effects 0.000 claims description 60
- 241000700605 Viruses Species 0.000 claims description 55
- 108020004414 DNA Proteins 0.000 claims description 53
- 101800002326 Adipokinetic hormone Proteins 0.000 claims description 45
- 101000634158 Androctonus australis Beta-insect excitatory toxin 1 Proteins 0.000 claims description 34
- 102000004169 proteins and genes Human genes 0.000 claims description 25
- 101150031823 HSP70 gene Proteins 0.000 claims description 24
- 101100125027 Dictyostelium discoideum mhsp70 gene Proteins 0.000 claims description 23
- 101150052825 dnaK gene Proteins 0.000 claims description 23
- 108091026890 Coding region Proteins 0.000 claims description 20
- 230000004048 modification Effects 0.000 claims description 20
- 238000012986 modification Methods 0.000 claims description 20
- 239000002773 nucleotide Substances 0.000 claims description 20
- 125000003729 nucleotide group Chemical group 0.000 claims description 20
- 241000255789 Bombyx mori Species 0.000 claims description 19
- 239000000463 material Substances 0.000 claims description 19
- 230000008034 disappearance Effects 0.000 claims description 18
- 235000013399 edible fruits Nutrition 0.000 claims description 18
- 230000008859 change Effects 0.000 claims description 16
- 238000010367 cloning Methods 0.000 claims description 16
- 230000000361 pesticidal effect Effects 0.000 claims description 11
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 9
- 101710182846 Polyhedrin Proteins 0.000 claims description 9
- -1 gricultural Species 0.000 claims description 9
- 150000007523 nucleic acids Chemical class 0.000 claims description 9
- 108010080576 juvenile hormone esterase Proteins 0.000 claims description 8
- 108091058549 μ-conotoxin Proteins 0.000 claims description 8
- KNJNGVKTAFTUFL-OCMUWRIYSA-N ω-conotoxin Chemical compound N([C@@H](CO)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@H]1C(N[C@@H](CSSC1)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H]1C(N[C@@H](CCCN=C(N)N)C(=O)N[C@H](CO)C(=O)NCC(=O)N[C@H](CCCCN)C(=O)N[C@H](CSSC1)C(N)=O)=O)=O)C(=O)[C@@H]1CSSC[C@@H](N)C(=O)N[C@H](CCCCN)C(=O)NCC(=O)N[C@H](CCCCN)C(=O)NCC(=O)N[C@H](C)C(=O)N[C@@H](CCCCN)C(=O)N1 KNJNGVKTAFTUFL-OCMUWRIYSA-N 0.000 claims description 8
- 108091058550 ω-conotoxin Proteins 0.000 claims description 8
- 101000634177 Androctonus australis Beta-insect excitatory toxin 2 Proteins 0.000 claims description 7
- 101710164760 Chlorotoxin Proteins 0.000 claims description 7
- QPAKKWCQMHUHNI-GQIQPHNSSA-N chlorotoxin Chemical compound C([C@H]1C(=O)NCC(=O)N2CCC[C@H]2C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@H]2CSSC[C@H]3C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@H]4CSSC[C@@H](C(N[C@@H](CCSC)C(=O)N5CCC[C@H]5C(=O)N[C@@H](CSSC[C@@H](C(=O)N1)NC(=O)[C@H](CCCCN)NC(=O)CNC(=O)[C@H](CCCNC(N)=N)NC(=O)CNC(=O)[C@H](CCCCN)NC(=O)CNC(=O)CNC(=O)[C@H](CSSC[C@H](NC(=O)[C@H](CC(C)C)NC2=O)C(=O)N[C@@H](CCCNC(N)=N)C(N)=O)NC4=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC=1N=CNC=1)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCCN)C(=O)N3)=O)NC(=O)[C@@H](N)CCSC)C1=CC=C(O)C=C1 QPAKKWCQMHUHNI-GQIQPHNSSA-N 0.000 claims description 7
- 229960005534 chlorotoxin Drugs 0.000 claims description 7
- 108091058551 α-conotoxin Proteins 0.000 claims description 7
- 101710166052 Apolipophorin Proteins 0.000 claims description 6
- 101710134987 Esterase 6 Proteins 0.000 claims description 6
- 210000001136 chorion Anatomy 0.000 claims description 6
- 230000002779 inactivation Effects 0.000 claims description 6
- 210000000434 stratum corneum Anatomy 0.000 claims description 6
- 108010085238 Actins Proteins 0.000 claims description 4
- 102000007469 Actins Human genes 0.000 claims description 4
- 108700005077 Viral Genes Proteins 0.000 claims description 3
- 238000013518 transcription Methods 0.000 claims 6
- 230000035897 transcription Effects 0.000 claims 6
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 claims 2
- 241000607479 Yersinia pestis Species 0.000 abstract description 6
- 206010061217 Infestation Diseases 0.000 abstract description 3
- 238000009472 formulation Methods 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 43
- 239000012634 fragment Substances 0.000 description 28
- 235000018102 proteins Nutrition 0.000 description 22
- 239000013604 expression vector Substances 0.000 description 21
- 230000003612 virological effect Effects 0.000 description 19
- 108020004705 Codon Proteins 0.000 description 18
- 208000015181 infectious disease Diseases 0.000 description 17
- 108091034117 Oligonucleotide Proteins 0.000 description 16
- 230000029087 digestion Effects 0.000 description 15
- 238000003752 polymerase chain reaction Methods 0.000 description 15
- 239000000047 product Substances 0.000 description 15
- 238000012360 testing method Methods 0.000 description 15
- 239000013598 vector Substances 0.000 description 14
- 238000006243 chemical reaction Methods 0.000 description 13
- 210000003000 inclusion body Anatomy 0.000 description 12
- 101150042515 DA26 gene Proteins 0.000 description 11
- 230000003321 amplification Effects 0.000 description 11
- 238000003199 nucleic acid amplification method Methods 0.000 description 11
- 108091008146 restriction endonucleases Proteins 0.000 description 11
- 101000798956 Hadronyche versuta Omega-hexatoxin-Hv1a Proteins 0.000 description 10
- 108010005774 beta-Galactosidase Proteins 0.000 description 10
- 235000013305 food Nutrition 0.000 description 10
- 238000003780 insertion Methods 0.000 description 10
- 230000037431 insertion Effects 0.000 description 10
- 230000034994 death Effects 0.000 description 9
- 239000013612 plasmid Substances 0.000 description 9
- 241000201370 Autographa californica nucleopolyhedrovirus Species 0.000 description 8
- 102000004190 Enzymes Human genes 0.000 description 8
- 108090000790 Enzymes Proteins 0.000 description 8
- 239000000969 carrier Substances 0.000 description 8
- 230000004087 circulation Effects 0.000 description 8
- 238000010276 construction Methods 0.000 description 8
- 238000005520 cutting process Methods 0.000 description 8
- 108020004707 nucleic acids Proteins 0.000 description 7
- 102000039446 nucleic acids Human genes 0.000 description 7
- 238000002360 preparation method Methods 0.000 description 7
- 238000013461 design Methods 0.000 description 6
- 238000010586 diagram Methods 0.000 description 6
- 230000006870 function Effects 0.000 description 6
- 230000006801 homologous recombination Effects 0.000 description 6
- 238000002744 homologous recombination Methods 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 108090000765 processed proteins & peptides Proteins 0.000 description 6
- 238000000746 purification Methods 0.000 description 6
- 239000000376 reactant Substances 0.000 description 6
- 210000001519 tissue Anatomy 0.000 description 6
- 238000012546 transfer Methods 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
- 101100425557 Rattus norvegicus Tle3 gene Proteins 0.000 description 5
- 108020005202 Viral DNA Proteins 0.000 description 5
- WQZGKKKJIJFFOK-FPRJBGLDSA-N beta-D-galactose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-FPRJBGLDSA-N 0.000 description 5
- 238000005457 optimization Methods 0.000 description 5
- 239000002245 particle Substances 0.000 description 5
- 239000000575 pesticide Substances 0.000 description 5
- 230000028327 secretion Effects 0.000 description 5
- 238000011144 upstream manufacturing Methods 0.000 description 5
- 208000003322 Coinfection Diseases 0.000 description 4
- 108010017826 DNA Polymerase I Proteins 0.000 description 4
- 102000004594 DNA Polymerase I Human genes 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 108091036066 Three prime untranslated region Proteins 0.000 description 4
- 235000001014 amino acid Nutrition 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 150000002061 ecdysteroids Chemical class 0.000 description 4
- 238000013467 fragmentation Methods 0.000 description 4
- 238000006062 fragmentation reaction Methods 0.000 description 4
- 235000013311 vegetables Nutrition 0.000 description 4
- 241001367049 Autographa Species 0.000 description 3
- 108091026898 Leader sequence (mRNA) Proteins 0.000 description 3
- 241000238709 Pyemotes tritici Species 0.000 description 3
- 101000658304 Pyemotes tritici Toxin TxP-I Proteins 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 238000000137 annealing Methods 0.000 description 3
- 244000309466 calf Species 0.000 description 3
- 239000000499 gel Substances 0.000 description 3
- 239000005556 hormone Substances 0.000 description 3
- 229940088597 hormone Drugs 0.000 description 3
- 238000001727 in vivo Methods 0.000 description 3
- 230000000670 limiting effect Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 230000014616 translation Effects 0.000 description 3
- 241000701366 unidentified nuclear polyhedrosis viruses Species 0.000 description 3
- 239000002435 venom Substances 0.000 description 3
- 231100000611 venom Toxicity 0.000 description 3
- 210000001048 venom Anatomy 0.000 description 3
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
- OPIFSICVWOWJMJ-AEOCFKNESA-N 5-bromo-4-chloro-3-indolyl beta-D-galactoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC=C(Br)C(Cl)=C12 OPIFSICVWOWJMJ-AEOCFKNESA-N 0.000 description 2
- 241000238895 Atrax robustus Species 0.000 description 2
- 241001203868 Autographa californica Species 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 241000193388 Bacillus thuringiensis Species 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 238000001712 DNA sequencing Methods 0.000 description 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 108091036060 Linker DNA Proteins 0.000 description 2
- 241000702625 Mink enteritis virus Species 0.000 description 2
- 241000629719 Oat dwarf virus Species 0.000 description 2
- 108700026244 Open Reading Frames Proteins 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 206010033799 Paralysis Diseases 0.000 description 2
- 108010021757 Polynucleotide 5'-Hydroxyl-Kinase Proteins 0.000 description 2
- 102000008422 Polynucleotide 5'-hydroxyl-kinase Human genes 0.000 description 2
- 229920002684 Sepharose Polymers 0.000 description 2
- 108020005038 Terminator Codon Proteins 0.000 description 2
- 108700009124 Transcription Initiation Site Proteins 0.000 description 2
- 125000003275 alpha amino acid group Chemical group 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 229940097012 bacillus thuringiensis Drugs 0.000 description 2
- 102000005936 beta-Galactosidase Human genes 0.000 description 2
- 239000012681 biocontrol agent Substances 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000004927 clay Substances 0.000 description 2
- 210000001072 colon Anatomy 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000001186 cumulative effect Effects 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000009849 deactivation Effects 0.000 description 2
- 230000030609 dephosphorylation Effects 0.000 description 2
- 238000006209 dephosphorylation reaction Methods 0.000 description 2
- 235000005911 diet Nutrition 0.000 description 2
- 230000037213 diet Effects 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 230000001079 digestive effect Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000001976 enzyme digestion Methods 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 239000012628 flowing agent Substances 0.000 description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 238000001502 gel electrophoresis Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000002458 infectious effect Effects 0.000 description 2
- 230000001524 infective effect Effects 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000005304 joining Methods 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 230000008018 melting Effects 0.000 description 2
- 238000010369 molecular cloning Methods 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 2
- 230000035479 physiological effects, processes and functions Effects 0.000 description 2
- 239000013600 plasmid vector Substances 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000001742 protein purification Methods 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 230000002194 synthesizing effect Effects 0.000 description 2
- 238000001890 transfection Methods 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- 230000029812 viral genome replication Effects 0.000 description 2
- 230000009385 viral infection Effects 0.000 description 2
- 239000004563 wettable powder Substances 0.000 description 2
- OJHZNMVJJKMFGX-RNWHKREASA-N (4r,4ar,7ar,12bs)-9-methoxy-3-methyl-1,2,4,4a,5,6,7a,13-octahydro-4,12-methanobenzofuro[3,2-e]isoquinoline-7-one;2,3-dihydroxybutanedioic acid Chemical compound OC(=O)C(O)C(O)C(O)=O.O=C([C@@H]1O2)CC[C@H]3[C@]4([H])N(C)CC[C@]13C1=C2C(OC)=CC=C1C4 OJHZNMVJJKMFGX-RNWHKREASA-N 0.000 description 1
- 241000142468 Bracon Species 0.000 description 1
- 125000001433 C-terminal amino-acid group Chemical group 0.000 description 1
- 108090000312 Calcium Channels Proteins 0.000 description 1
- 102000003922 Calcium Channels Human genes 0.000 description 1
- KXDHJXZQYSOELW-UHFFFAOYSA-N Carbamic acid Chemical compound NC(O)=O KXDHJXZQYSOELW-UHFFFAOYSA-N 0.000 description 1
- 239000004215 Carbon black (E152) Substances 0.000 description 1
- 206010057248 Cell death Diseases 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 230000004543 DNA replication Effects 0.000 description 1
- 241000537219 Deltabaculovirus Species 0.000 description 1
- 240000007790 Donax canniformis Species 0.000 description 1
- 241000255601 Drosophila melanogaster Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- VEPBEGNDJYANCF-QWRGUYRKSA-N Gly-Lys-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)CN)CCCCN VEPBEGNDJYANCF-QWRGUYRKSA-N 0.000 description 1
- 241000238894 Hadronyche versuta Species 0.000 description 1
- 241001147381 Helicoverpa armigera Species 0.000 description 1
- 102100024319 Intestinal-type alkaline phosphatase Human genes 0.000 description 1
- 101710184243 Intestinal-type alkaline phosphatase Proteins 0.000 description 1
- 241000239268 Leiurus quinquestriatus Species 0.000 description 1
- 241000255777 Lepidoptera Species 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 241000255908 Manduca sexta Species 0.000 description 1
- 206010027336 Menstruation delayed Diseases 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 241000428199 Mustelinae Species 0.000 description 1
- 241000244206 Nematoda Species 0.000 description 1
- 108090000189 Neuropeptides Proteins 0.000 description 1
- 102000003797 Neuropeptides Human genes 0.000 description 1
- 101100065105 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) egt-1 gene Proteins 0.000 description 1
- 101710138657 Neurotoxin Proteins 0.000 description 1
- 241000256259 Noctuidae Species 0.000 description 1
- 108010036939 Occlusion Body Matrix Proteins Proteins 0.000 description 1
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 description 1
- 208000033952 Paralysis flaccid Diseases 0.000 description 1
- 108010002747 Pfu DNA polymerase Proteins 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
- 241001674048 Phthiraptera Species 0.000 description 1
- 101710093543 Probable non-specific lipid-transfer protein Proteins 0.000 description 1
- 244000294611 Punica granatum Species 0.000 description 1
- 101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 241000239226 Scorpiones Species 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 108010052164 Sodium Channels Proteins 0.000 description 1
- 102000018674 Sodium Channels Human genes 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
- 239000004141 Sodium laurylsulphate Substances 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 241000219793 Trifolium Species 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- HMNZFMSWFCAGGW-XPWSMXQVSA-N [3-[hydroxy(2-hydroxyethoxy)phosphoryl]oxy-2-[(e)-octadec-9-enoyl]oxypropyl] (e)-octadec-9-enoate Chemical compound CCCCCCCC\C=C\CCCCCCCC(=O)OCC(COP(O)(=O)OCCO)OC(=O)CCCCCCC\C=C\CCCCCCCC HMNZFMSWFCAGGW-XPWSMXQVSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 101150099105 alien gene Proteins 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 239000010775 animal oil Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 230000035584 blastogenesis Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000034303 cell budding Effects 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- AIMMVWOEOZMVMS-UHFFFAOYSA-N cyclopropanecarboxamide Chemical compound NC(=O)C1CC1 AIMMVWOEOZMVMS-UHFFFAOYSA-N 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 235000013681 dietary sucrose Nutrition 0.000 description 1
- FPAFDBFIGPHWGO-UHFFFAOYSA-N dioxosilane;oxomagnesium;hydrate Chemical compound O.[Mg]=O.[Mg]=O.[Mg]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O FPAFDBFIGPHWGO-UHFFFAOYSA-N 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000010410 dusting Methods 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 230000012202 endocytosis Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000000967 entomopathogenic effect Effects 0.000 description 1
- HQPMKSGTIOYHJT-UHFFFAOYSA-N ethane-1,2-diol;propane-1,2-diol Chemical compound OCCO.CC(O)CO HQPMKSGTIOYHJT-UHFFFAOYSA-N 0.000 description 1
- 239000004060 excitotoxin Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 230000004634 feeding behavior Effects 0.000 description 1
- 208000028331 flaccid paralysis Diseases 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 210000000087 hemolymph Anatomy 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- PHTQWCKDNZKARW-UHFFFAOYSA-N isoamylol Chemical compound CC(C)CCO PHTQWCKDNZKARW-UHFFFAOYSA-N 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 231100000225 lethality Toxicity 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 239000002480 mineral oil Substances 0.000 description 1
- 235000010446 mineral oil Nutrition 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000002581 neurotoxin Substances 0.000 description 1
- 231100000618 neurotoxin Toxicity 0.000 description 1
- 238000001807 normal pulse voltammetry Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 238000002515 oligonucleotide synthesis Methods 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 208000021090 palsy Diseases 0.000 description 1
- 230000001769 paralizing effect Effects 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 239000000813 peptide hormone Substances 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 229920001993 poloxamer 188 Polymers 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000003223 protective agent Substances 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- 150000003219 pyrazolines Chemical class 0.000 description 1
- 239000002728 pyrethroid Substances 0.000 description 1
- 239000011535 reaction buffer Substances 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 108010025960 scorpion toxin AaH IT2 Proteins 0.000 description 1
- 239000002795 scorpion venom Substances 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 238000001542 size-exclusion chromatography Methods 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 229960004793 sucrose Drugs 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- 210000002845 virion Anatomy 0.000 description 1
- 239000000277 virosome Substances 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
- 230000005727 virus proliferation Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
- C07K14/43531—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from mites
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/40—Viruses, e.g. bacteriophages
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/14011—Baculoviridae
- C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
- C12N2710/14141—Use of virus, viral particle or viral elements as a vector
- C12N2710/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Insects & Arthropods (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Toxicology (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US8470598P | 1998-05-08 | 1998-05-08 | |
| US60/084,705 | 1998-05-08 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1310767A true CN1310767A (zh) | 2001-08-29 |
Family
ID=22186700
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN99807162A Pending CN1310767A (zh) | 1998-05-08 | 1999-05-07 | 基于重组杆状病毒的杀虫剂 |
Country Status (16)
| Country | Link |
|---|---|
| EP (1) | EP1076717A1 (sk) |
| JP (1) | JP2002514435A (sk) |
| KR (1) | KR20010043627A (sk) |
| CN (1) | CN1310767A (sk) |
| AU (1) | AU753930B2 (sk) |
| BR (1) | BR9910515A (sk) |
| CA (1) | CA2331853A1 (sk) |
| HU (1) | HUP0101684A3 (sk) |
| ID (1) | ID28101A (sk) |
| IL (1) | IL139401A0 (sk) |
| NZ (1) | NZ507918A (sk) |
| PL (1) | PL345242A1 (sk) |
| SK (1) | SK16852000A3 (sk) |
| TR (1) | TR200003245T2 (sk) |
| WO (1) | WO1999058705A1 (sk) |
| ZA (1) | ZA200006301B (sk) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1313604C (zh) * | 2004-09-22 | 2007-05-02 | 山西大学 | 一种含有双价抗虫基因的重组杆状病毒 |
| CN105441469A (zh) * | 2016-01-15 | 2016-03-30 | 西南大学 | 重组昆虫蜕皮激素失活基因Bbsp::egt及其杀虫真菌剂 |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7279547B2 (en) | 2000-02-10 | 2007-10-09 | University Of Connecticut | Insecticidal compounds and methods for selection thereof |
| US6583264B2 (en) | 2000-02-10 | 2003-06-24 | University Of Connecticut | Insecticidal compounds and methods for selection thereof |
| KR100594353B1 (ko) * | 2002-05-28 | 2006-07-03 | 주식회사 엠디바이오알파 | 신규한 인삼잎 및 줄기 다당체, 그 제조방법 및 그를활성성분으로 함유하는 항암제 및 항암보조제 조성물 |
| CA2622436A1 (en) * | 2005-09-16 | 2007-03-29 | University Of Connecticut | Acaricidal compositions and methods of use thereof |
| US20110201086A1 (en) | 2010-02-12 | 2011-08-18 | Otsuka Pharmaceutical Co., Ltd. | Method for producing recombinant virus |
| GB201105418D0 (en) * | 2011-03-31 | 2011-05-18 | Univ Durham | Pesticide |
| CN103409430B (zh) * | 2013-08-19 | 2014-08-20 | 南京师范大学 | 小菜蛾泛素基因ubl40及在治理小菜蛾的溴氰菊酯抗性中的应用 |
| GB201321938D0 (en) | 2013-12-11 | 2014-01-22 | Univ Durham | Pesticidal fusion protein improvements |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5674485A (en) * | 1988-11-01 | 1997-10-07 | The Regents Of The University Of California | Insect diagnostic and control compositions with truncated JHE |
| US5461032A (en) * | 1991-03-01 | 1995-10-24 | Fmc Corporation | Insecticidally effective peptides |
| IL104419A0 (en) * | 1992-01-24 | 1993-05-13 | Fmc Corp | Insecticidally effective peptides |
| US5457178A (en) * | 1993-07-07 | 1995-10-10 | Fmc Corporation | Insecticidally effective spider toxin |
-
1999
- 1999-05-07 PL PL99345242A patent/PL345242A1/xx unknown
- 1999-05-07 CA CA002331853A patent/CA2331853A1/en not_active Abandoned
- 1999-05-07 CN CN99807162A patent/CN1310767A/zh active Pending
- 1999-05-07 NZ NZ507918A patent/NZ507918A/en unknown
- 1999-05-07 SK SK1685-2000A patent/SK16852000A3/sk unknown
- 1999-05-07 BR BR9910515-2A patent/BR9910515A/pt not_active IP Right Cessation
- 1999-05-07 TR TR2000/03245T patent/TR200003245T2/xx unknown
- 1999-05-07 EP EP99921724A patent/EP1076717A1/en not_active Withdrawn
- 1999-05-07 IL IL13940199A patent/IL139401A0/xx unknown
- 1999-05-07 AU AU38856/99A patent/AU753930B2/en not_active Ceased
- 1999-05-07 JP JP2000548496A patent/JP2002514435A/ja active Pending
- 1999-05-07 ID IDW20002404A patent/ID28101A/id unknown
- 1999-05-07 WO PCT/US1999/009914 patent/WO1999058705A1/en not_active Application Discontinuation
- 1999-05-07 KR KR1020007012790A patent/KR20010043627A/ko not_active Application Discontinuation
- 1999-05-07 HU HU0101684A patent/HUP0101684A3/hu unknown
-
2000
- 2000-11-03 ZA ZA200006301A patent/ZA200006301B/xx unknown
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1313604C (zh) * | 2004-09-22 | 2007-05-02 | 山西大学 | 一种含有双价抗虫基因的重组杆状病毒 |
| CN105441469A (zh) * | 2016-01-15 | 2016-03-30 | 西南大学 | 重组昆虫蜕皮激素失活基因Bbsp::egt及其杀虫真菌剂 |
| CN105441469B (zh) * | 2016-01-15 | 2018-08-07 | 西南大学 | 重组昆虫蜕皮激素失活基因Bbsp::egt及其杀虫真菌剂 |
Also Published As
| Publication number | Publication date |
|---|---|
| TR200003245T2 (tr) | 2001-03-21 |
| IL139401A0 (en) | 2001-11-25 |
| WO1999058705A1 (en) | 1999-11-18 |
| HUP0101684A2 (hu) | 2001-08-28 |
| NZ507918A (en) | 2002-07-26 |
| BR9910515A (pt) | 2003-04-15 |
| KR20010043627A (ko) | 2001-05-25 |
| ID28101A (id) | 2001-05-03 |
| AU3885699A (en) | 1999-11-29 |
| SK16852000A3 (sk) | 2001-10-08 |
| CA2331853A1 (en) | 1999-11-18 |
| JP2002514435A (ja) | 2002-05-21 |
| AU753930B2 (en) | 2002-10-31 |
| PL345242A1 (en) | 2001-12-03 |
| HUP0101684A3 (en) | 2003-08-28 |
| EP1076717A1 (en) | 2001-02-21 |
| ZA200006301B (en) | 2001-06-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US5266317A (en) | Insect-specific paralytic neurotoxin genes for use in biological insect control: methods and compositions | |
| Szewczyk et al. | Baculoviruses—re-emerging biopesticides | |
| CN1102659C (zh) | 重组杆状病毒杀虫剂 | |
| CN1161458C (zh) | 重组杆状病毒及其构建方法，以及含该病毒的杀虫组合物 | |
| AU692290B2 (en) | Insect viruses, sequences, insecticidal compositions and methods of use | |
| CN1112105C (zh) | 用复合毒素防治昆虫 | |
| TW397865B (en) | Heterologous signal sequences for secretion of insect controlling proteins | |
| CN1310767A (zh) | 基于重组杆状病毒的杀虫剂 | |
| Haase et al. | Genetic engineering of baculoviruses | |
| Mishra | Baculoviruses as biopesticides | |
| AU743526B2 (en) | Transgenic virus | |
| EP0621337A1 (en) | Codon optimized DNA sequence for insect toxin AaIT | |
| JP2000507089A (ja) | 組換えバキュロウイルスの生産 | |
| US6090379A (en) | Stable pre-occluded virus particle for use in recombinant protein production and pesticides | |
| US6087165A (en) | Recombinant baculovirus and its use as a biocontrol agent for crop pests | |
| AU675939B2 (en) | Recombinant insect virus with reduced capacity for host-to-host transmission in the environment and methods to produce said virus | |
| US6521454B1 (en) | Baculoviruses, insecticidal compositions, and methods for control of invertebrates | |
| MXPA00010981A (es) | Insecticidas con base en basculovirus recombinantes | |
| JP2002306167A (ja) | 形質転換カイコ作製用ベクター | |
| AU720082B2 (en) | Heterologous signal sequences for secretion of insect controlling toxins | |
| US5593669A (en) | Stable pre-occluded virus particle | |
| AU721364B2 (en) | Codon optimized DNA sequence for insect toxin AaIT | |
| CZ20004156A3 (cs) | Insekticidy na bázi rekombinantního bakuloviru |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |