CN1301327C - 人肿瘤细胞的分离纯化方法 - Google Patents
人肿瘤细胞的分离纯化方法 Download PDFInfo
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- CN1301327C CN1301327C CNB031469337A CN03146933A CN1301327C CN 1301327 C CN1301327 C CN 1301327C CN B031469337 A CNB031469337 A CN B031469337A CN 03146933 A CN03146933 A CN 03146933A CN 1301327 C CN1301327 C CN 1301327C
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- 210000004881 tumor cell Anatomy 0.000 title claims abstract description 67
- 238000000034 method Methods 0.000 title claims abstract description 16
- 210000004027 cell Anatomy 0.000 claims abstract description 102
- 210000002966 serum Anatomy 0.000 claims abstract description 64
- 206010028980 Neoplasm Diseases 0.000 claims abstract description 55
- 238000000746 purification Methods 0.000 claims abstract description 31
- 238000000926 separation method Methods 0.000 claims abstract description 30
- 239000000725 suspension Substances 0.000 claims abstract description 12
- 239000012980 RPMI-1640 medium Substances 0.000 claims description 38
- 239000000243 solution Substances 0.000 claims description 37
- 239000006285 cell suspension Substances 0.000 claims description 26
- 235000015097 nutrients Nutrition 0.000 claims description 24
- 239000006228 supernatant Substances 0.000 claims description 23
- 206010006187 Breast cancer Diseases 0.000 claims description 14
- 208000026310 Breast neoplasm Diseases 0.000 claims description 14
- 206010038389 Renal cancer Diseases 0.000 claims description 14
- 210000001638 cerebellum Anatomy 0.000 claims description 12
- 206010058467 Lung neoplasm malignant Diseases 0.000 claims description 11
- 201000005296 lung carcinoma Diseases 0.000 claims description 11
- 230000009849 deactivation Effects 0.000 claims description 10
- 238000002360 preparation method Methods 0.000 claims description 10
- 208000000172 Medulloblastoma Diseases 0.000 claims description 9
- 101001011890 Xenopus laevis Matrix metalloproteinase-18 Proteins 0.000 claims description 9
- 238000001914 filtration Methods 0.000 claims description 9
- 102000029816 Collagenase Human genes 0.000 claims description 8
- 108060005980 Collagenase Proteins 0.000 claims description 8
- 229960002424 collagenase Drugs 0.000 claims description 8
- 238000010790 dilution Methods 0.000 claims description 8
- 239000012895 dilution Substances 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000009736 wetting Methods 0.000 claims description 8
- 208000006265 Renal cell carcinoma Diseases 0.000 claims description 7
- 201000010174 renal carcinoma Diseases 0.000 claims description 7
- 230000002611 ovarian Effects 0.000 claims description 4
- 230000035755 proliferation Effects 0.000 claims description 4
- 208000000461 Esophageal Neoplasms Diseases 0.000 claims 1
- 206010030155 Oesophageal carcinoma Diseases 0.000 claims 1
- 230000029087 digestion Effects 0.000 claims 1
- 210000002919 epithelial cell Anatomy 0.000 claims 1
- 201000004101 esophageal cancer Diseases 0.000 claims 1
- 208000005017 glioblastoma Diseases 0.000 claims 1
- 239000002054 inoculum Substances 0.000 claims 1
- 201000011061 large intestine cancer Diseases 0.000 claims 1
- 230000003211 malignant effect Effects 0.000 claims 1
- 238000011160 research Methods 0.000 abstract description 7
- 239000002246 antineoplastic agent Substances 0.000 abstract description 4
- 229940041181 antineoplastic drug Drugs 0.000 abstract description 4
- 239000000463 material Substances 0.000 abstract description 2
- 238000002156 mixing Methods 0.000 description 18
- 230000001954 sterilising effect Effects 0.000 description 14
- 230000001464 adherent effect Effects 0.000 description 12
- 208000002454 Nasopharyngeal Carcinoma Diseases 0.000 description 11
- 206010061306 Nasopharyngeal cancer Diseases 0.000 description 11
- 201000011216 nasopharynx carcinoma Diseases 0.000 description 11
- 206010033128 Ovarian cancer Diseases 0.000 description 10
- 206010061535 Ovarian neoplasm Diseases 0.000 description 10
- 208000035269 cancer or benign tumor Diseases 0.000 description 9
- 230000017074 necrotic cell death Effects 0.000 description 9
- 210000004882 non-tumor cell Anatomy 0.000 description 9
- 208000008839 Kidney Neoplasms Diseases 0.000 description 7
- 238000012258 culturing Methods 0.000 description 7
- 239000000645 desinfectant Substances 0.000 description 7
- 201000010982 kidney cancer Diseases 0.000 description 7
- 238000004659 sterilization and disinfection Methods 0.000 description 7
- 210000004072 lung Anatomy 0.000 description 5
- 201000011510 cancer Diseases 0.000 description 3
- 238000004113 cell culture Methods 0.000 description 3
- 210000000130 stem cell Anatomy 0.000 description 3
- 210000004698 lymphocyte Anatomy 0.000 description 2
- 244000309466 calf Species 0.000 description 1
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Abstract
Description
Claims (7)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031469337A CN1301327C (zh) | 2003-09-25 | 2003-09-25 | 人肿瘤细胞的分离纯化方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031469337A CN1301327C (zh) | 2003-09-25 | 2003-09-25 | 人肿瘤细胞的分离纯化方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1526814A CN1526814A (zh) | 2004-09-08 |
| CN1301327C true CN1301327C (zh) | 2007-02-21 |
Family
ID=34286684
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB031469337A Expired - Fee Related CN1301327C (zh) | 2003-09-25 | 2003-09-25 | 人肿瘤细胞的分离纯化方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1301327C (zh) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW200829269A (en) * | 2007-01-12 | 2008-07-16 | Koyo Internat Holding Corp | Methods for the cultivation of DC/D-CIK cells and applications |
| CN101245337B (zh) * | 2008-02-25 | 2011-07-06 | 浙江大学医学院附属邵逸夫医院 | 维持大肠癌肿瘤细胞短期生长的原代培养方法 |
| CN108070560A (zh) * | 2016-11-15 | 2018-05-25 | 江苏齐氏生物科技有限公司 | 一种人原代胃癌细胞的分离及培养方法 |
| CN111117965A (zh) * | 2020-01-08 | 2020-05-08 | 宁波市医疗中心李惠利医院 | 一种高纯原代肿瘤细胞的快速纯化方法 |
| CN112852729A (zh) * | 2021-04-16 | 2021-05-28 | 天津市环湖医院 | 一种从骨髓、外周血以及淋巴瘤组织中提取高质量b细胞的方法 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1249000A (zh) * | 1997-01-24 | 2000-03-29 | 旭医学株式会社 | 细胞分离方法 |
-
2003
- 2003-09-25 CN CNB031469337A patent/CN1301327C/zh not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1249000A (zh) * | 1997-01-24 | 2000-03-29 | 旭医学株式会社 | 细胞分离方法 |
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| Publication number | Publication date |
|---|---|
| CN1526814A (zh) | 2004-09-08 |
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